Srd5a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srd5a2
Entrez Gene ID	64677
Full Name	steroid 5 alpha-reductase 2
Synonyms	S5AR 2
General protein information	Preferred Names steroid 5 alpha-reductase 2 Names 3-oxo-5-alpha-steroid 4-dehydrogenase 2 5 alpha-SR2 SR type 2 steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	enzyme which converts testosterone to dihydrotestosterone (DHT); also acts on progesterone and corticosterone; plays a central role in sexual differentiation and androgen physiology [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022711.4	NP_073202.1	3-oxo-5-alpha-steroid 4-dehydrogenase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SRD5A2	NP_000339.2
Rattus norvegicus (Norway rat)	Srd5a2	NP_073202.1
Mus musculus (house mouse)	Srd5a2	NP_444418.1
Gallus gallus (chicken)	SRD5A2	XP_001235447.1
Caenorhabditis elegans (roundworm)	CELE_ZK1251.3	NP_501719.1
	SRD5A2	XP_001105329.1
Canis lupus familiaris (dog)	SRD5A2	XP_532922.2
Bos taurus (cattle)	SRD5A2	NP_001192866.1
Danio rerio (zebrafish)	srd5a2a	NP_001017703.1
Caenorhabditis elegans (roundworm)	F19H6.4	NP_510077.1
Caenorhabditis elegans (roundworm)	CELE_F42F12.3	NP_510071.1
Xenopus tropicalis (tropical clawed frog)	srd5a2	NP_001017113.1
Pan troglodytes (chimpanzee)	SRD5A2	XP_003309033.1
Danio rerio (zebrafish)	srd5a2b	NP_001017693.1

Gene Ontology
Bibliography

Related articles in PubMed

Di-(2-ethylhexyl) phthalate inhibits testosterone level through disturbed hypothalamic-pituitary-testis axis and ERK-mediated 5?-Reductase 2.
Ha M, Guan X, Wei L, Li P, Yang M, Liu C
The Science of the total environment563-564566-75(2016 Sep)

Temporal changes in the expression of the translocator protein TSPO and the steroidogenic enzyme 5?-reductase in the dorsal spinal cord of animals with neuropathic pain: Effects of progesterone administration.
Coronel MF, S?nchez Granel ML, Raggio MC, Adler NS, De Nicola AF, Labombarda F, Gonz?lez SL
Neuroscience letters62423-8(2016 Jun)

Regional distribution of 5?-reductase type 2 in the adult rat brain: an immunohistochemical analysis.
Castelli MP, Casti A, Casu A, Frau R, Bortolato M, Spiga S, Ennas MG
Psychoneuroendocrinology38(2)281-93(2013 Feb)

Expression of steroid 5?-reductase isozymes in prostate of adult rats after environmental stress.
S?nchez P, Torres JM, Castro B, Olmo A, del Moral RG, Ortega E
The FEBS journal280(1)93-101(2013 Jan)

Bisphenol A exposure during adulthood alters expression of aromatase and 5?-reductase isozymes in rat prostate.
Castro B, S?nchez P, Torres JM, Preda O, del Moral RG, Ortega E
PloS one8(2)e55905(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that in the acute phase after spinal cord injury, the increased expression of 18kDa translocator protein and 5alpha-reductase may represent a protective endogenous response against tissue injury.
Title: Temporal changes in the expression of the translocator protein TSPO and the steroidogenic enzyme 5?-reductase in the dorsal spinal cord of animals with neuropathic pain: Effects of progesterone administration.

findings show that, in the adult brain, 5alphaR2 is distributed in critical regions for behavioral regulation, suggesting that the functional role of this isoform is present throughout the entire lifespan of the individual
Title: Regional distribution of 5?-reductase type 2 in the adult rat brain: an immunohistochemical analysis.

Changes in the expression of steroid 5-alpha reductase isoenzymes in the prostate in response to environmental stress.
Title: Expression of steroid 5?-reductase isozymes in prostate of adult rats after environmental stress.

Low Srd5a2 expression is associated with benign prostatic hypertrophy.
Title: Bisphenol A exposure during adulthood alters expression of aromatase and 5?-reductase isozymes in rat prostate.

Tesosterone concentrations during postnatal sexual differentiation of the rat central nervous system, among other sex-dependent factors, influence brain levels of 5alpha-Reductase isozymes in adulthood
Title: Steroid 5?-reductase in adult rat brain after neonatal testosterone administration.

The following Srd5a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srd5a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12642	NM_022711.4 Latest version!	Rattus norvegicus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srd5a2 ( NM_022711.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073202.1 Rattus norvegicus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.

Price & Availability↑

CloneID	ORa12642
Clone ID Related Accession (Same CDS sequence)	NM_022711.4
Accession Version	NM_022711.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M95058.1. On or before Jun 26, 2012 this sequence version replaced XM_003750114.1, NM_022711.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95058.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMEA2689595 [ECO:0000350] ##Evidence-Data-END##

ATGCAGATTG TCTGCCATCA GGTCCCGGTG CTGGCAGGTA GCGCCACATT GGCCACTATG 
GGGACCCTGA TCCTGTGCTT AGGGAAACCC GCCAGTTACG GGAAACACAC AGAGAGTGTG 
TCGTCGGGAG TTCCCTTCCT GCCGGCACGC ATCGCCTGGT TCCTGCAGGA GTTGCCTTCC 
TTTGTGGTGT CGGTAGGGAT GCTCGCTTGG CAGCCGCGCT CCCTCTTCGG ACCGCCCGGG 
AATGTCCTGC TGGCTCTCTT CTCTGCACAT TACTTCCACA GGACATTTAT TTACTCGTTG 
CTCACAAGAG GGAGGCCTTT CCCAGCGGTG CTGTTTTTGA GAGCCACTGC CTTCTGCATA 
GGGAACGGAC TCCTTCAAGC CTACTACCTG GTTTACTGCG CAGAATACCC CGAGGAGTGG 
TACACAGATG TGCGGTTTAG CTTTGGTGTC TTCCTGTTTA TTCTGGGGAT GGGAATCAAC 
ATCCACAGTG ACTACACCCT GCGCCAGCTC AGGAAGCCTG GAGAAGTCAT CTATAGGATT 
CCTCGAGGTG GCTTGTTTAC GTATGTCTCT GGAGCCAATT TCCTGGGCGA GATTATTGAA 
TGGATTGGCT ACGCCTTGGC CACGTGGTCC GTCCCAGCCT TCGCTTTCGC CTTTTTCACA 
CTTTGTTTCC TGGGGATGCA AGCCTTTTAC CACCACAGGT TCTACCTTAA GATGTTTAAG 
GATTACCCCA AATCTAGGAA AGCTCTCATT CCATTCATCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073202.1
CDS	48..812
Translation	MQIVCHQVPVLAGSATLATMGTLILCLGKPASYGKHTESVSSGVPFLPARIAWFLQELPSFVVSVGMLAWQPRSLFGPPGNVLLALFSAHYFHRTFIYSLLTRGRPFPAVLFLRATAFCIGNGLLQAYYLVYCAEYPEEWYTDVRFSFGVFLFILGMGINIHSDYTLRQLRKPGEVIYRIPRGGLFTYVSGANFLGEIIEWIGYALATWSVPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF

Target ORF information:

RefSeq Version	NM_022711.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022711.4

ORF Insert Sequence:

ATGCAGATTG TCTGCCATCA GGTCCCGGTG CTGGCAGGTA GCGCCACATT GGCCACTATG 
GGGACCCTGA TCCTGTGCTT AGGGAAACCC GCCAGTTACG GGAAACACAC AGAGAGTGTG 
TCGTCGGGAG TTCCCTTCCT GCCGGCACGC ATCGCCTGGT TCCTGCAGGA GTTGCCTTCC 
TTTGTGGTGT CGGTAGGGAT GCTCGCTTGG CAGCCGCGCT CCCTCTTCGG ACCGCCCGGG 
AATGTCCTGC TGGCTCTCTT CTCTGCACAT TACTTCCACA GGACATTTAT TTACTCGTTG 
CTCACAAGAG GGAGGCCTTT CCCAGCGGTG CTGTTTTTGA GAGCCACTGC CTTCTGCATA 
GGGAACGGAC TCCTTCAAGC CTACTACCTG GTTTACTGCG CAGAATACCC CGAGGAGTGG 
TACACAGATG TGCGGTTTAG CTTTGGTGTC TTCCTGTTTA TTCTGGGGAT GGGAATCAAC 
ATCCACAGTG ACTACACCCT GCGCCAGCTC AGGAAGCCTG GAGAAGTCAT CTATAGGATT 
CCTCGAGGTG GCTTGTTTAC GTATGTCTCT GGAGCCAATT TCCTGGGCGA GATTATTGAA 
TGGATTGGCT ACGCCTTGGC CACGTGGTCC GTCCCAGCCT TCGCTTTCGC CTTTTTCACA 
CTTTGTTTCC TGGGGATGCA AGCCTTTTAC CACCACAGGT TCTACCTTAA GATGTTTAAG 
GATTACCCCA AATCTAGGAA AGCTCTCATT CCATTCATCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Di-(2-ethylhexyl) phthalate inhibits testosterone level through disturbed hypothalamic-pituitary-testis axis and ERK-mediated 5?-Reductase 2.
The Science of the total environment563-564566-75(2016 Sep)
Ha M,Guan X,Wei L,Li P,Yang M,Liu C

Temporal changes in the expression of the translocator protein TSPO and the steroidogenic enzyme 5?-reductase in the dorsal spinal cord of animals with neuropathic pain: Effects of progesterone administration.
Neuroscience letters62423-8(2016 Jun)
Coronel MF,S?nchez Granel ML,Raggio MC,Adler NS,De Nicola AF,Labombarda F,Gonz?lez SL

Regional distribution of 5?-reductase type 2 in the adult rat brain: an immunohistochemical analysis.
Psychoneuroendocrinology38(2)281-93(2013 Feb)
Castelli MP,Casti A,Casu A,Frau R,Bortolato M,Spiga S,Ennas MG

Expression of steroid 5?-reductase isozymes in prostate of adult rats after environmental stress.
The FEBS journal280(1)93-101(2013 Jan)
S?nchez P,Torres JM,Castro B,Olmo A,del Moral RG,Ortega E

Bisphenol A exposure during adulthood alters expression of aromatase and 5?-reductase isozymes in rat prostate.
PloS one8(2)e55905(2013)
Castro B,S?nchez P,Torres JM,Preda O,del Moral RG,Ortega E