Aldh1l1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aldh1l1
Entrez Gene ID	64392
Full Name	aldehyde dehydrogenase 1 family, member L1
Synonyms	FDH,Fthfd
General protein information	Preferred Names aldehyde dehydrogenase 1 family, member L1 Names cytosolic 10-formyltetrahydrofolate dehydrogenase 10-FTHFDH 10-formyltetrahydrofolate dehydrogenase FBP-CI aldehyde dehydrogenase family 1 member L1 formyltetrahydrofolate dehydrogenase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	catalyzes the NADP dependent oxidation of 10-HCO-H4PteGlu to H4PteGlu and Co2 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022547.1	NP_071992.1	cytosolic 10-formyltetrahydrofolate dehydrogenase

Pathways from BioSystems

Homologies

	ALDH1L1	XP_002802729.1
Mus musculus (house mouse)	Aldh1l1	NP_081682.1
Homo sapiens (human)	ALDH1L1	NP_001257293.1
Bos taurus (cattle)	ALDH1L1	NP_001096557.1
Pan troglodytes (chimpanzee)	ALDH1L1	XP_516714.4
Rattus norvegicus (Norway rat)	Aldh1l1	NP_071992.1
Danio rerio (zebrafish)	aldh1l1	NP_001185701.1
Caenorhabditis elegans (roundworm)	alh-3	NP_502054.2
Xenopus tropicalis (tropical clawed frog)	aldh1l1	NP_001011027.1

Gene Ontology
Bibliography

Related articles in PubMed

Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase.
Strickland KC, Krupenko NI, Dubard ME, Hu CJ, Tsybovsky Y, Krupenko SA
Chemico-biological interactions191(1-3)129-36(2011 May)

Polymorphisms in 1-carbon metabolism, epigenetics and folate-related pathologies.
Stover PJ
Journal of nutrigenetics and nutrigenomics4(5)293-305(2011)

ALDH1L2 is the mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase.
Krupenko NI, Dubard ME, Strickland KC, Moxley KM, Oleinik NV, Krupenko SA
The Journal of biological chemistry285(30)23056-63(2010 Jul)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Rat liver 10-formyltetrahydrofolate dehydrogenase, carbamoyl phosphate synthetase 1 and betaine homocysteine S-methytransferase were co-purified on Kunitz-type soybean trypsin inhibitor-coupled sepharose CL-4B.
Kim HS, Kim JM, Roh KB, Lee HH, Kim SJ, Shin YH, Lee BL
Journal of biochemistry and molecular biology40(4)604-9(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Upon binding of NADP+, side chain of Glu673 is displaced from the active site by the nicotinamide ring & contacts a chain of highly ordered water molecules that may represent a pathway for translocation of the abstracted proton from Glu673 to the solvent.
Title: Crystal structures of the carboxyl terminal domain of rat 10-formyltetrahydrofolate dehydrogenase: implications for the catalytic mechanism of aldehyde dehydrogenases.

catalytic mechanism for the hydrolase reaction in which Asp-142 polarizes the catalytic water molecule and His-106 orients the carbonyl group of formyl.
Title: The crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase: mechanism of hydrolysis and its interplay with the dehydrogenase domain.

The following Aldh1l1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aldh1l1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42418	NM_022547.1 Latest version!	Rattus norvegicus aldehyde dehydrogenase 1 family, member L1 (Aldh1l1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aldh1l1 ( NM_022547.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071992.1 Rattus norvegicus aldehyde dehydrogenase 1 family, member L1 (Aldh1l1), mRNA.

Price & Availability↑

CloneID	ORa42418
Clone ID Related Accession (Same CDS sequence)	NM_022547.1
Accession Version	NM_022547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	cytosolic 10-formyltetrahydrofolate dehydrogenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M59861.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M59861.1, BC089101.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052297, SAMEA2689595 [ECO:0000350] ##Evidence-Data-END##

ATGAAGATTG CAGTAATCGG ACAGAGCCTG TTTGGCCAGG AGGTTTACTG CCAGCTGAGG 
AAGGAGGGCC ACGAGGTGGT GGGTGTGTTC ACAATCCCAG ACAAGGATGG GAAAGCAGAC 
CCCCTGGGCC TGGAGGCTGA GAAGGATGGC CGTGCCGTGT TCAAGTTTCC TCGGTGGCGT 
GCTCGGGGAC AGGCTCTGCC CGAGGTGGTG GCCAAATACC AGGCTCTGGG TGCAGAGCTC 
AATGTGCTTC CCTTCTGCAG CCAGTTCATA CCCATGGAGG TCATTAATGC CCCCAGGCAC 
GGCTCCATCA TCTACCATCC ATCCCTGCTA CCCAGGCATC GAGGGGCTTC GGCCATCAAC 
TGGACCCTCA TTCATGGAGA TAAGAAAGGA GGCTTCACCA TCTTCTGGGC TGATGATGGC 
CTGGACACTG GTGACCTTCT GCTGCAGAAG GAGTGTGAGG TGCTTCCAGA TGACACCGTG 
AGCACGCTGT ACAATCGTTT CCTCTTCCCA GAGGGCATCA AAGGGATGGT TCAGGCTGTG 
CGACTGATCG CAGAGGGTAC AGCCCCCCGC TGCCCCCAGT CTGAGGAAGG AGCCACCTAT 
GAGGGCATTC AGAAGAAGGA GACAGCCAAG ATCAATTGGG ACCAGCCAGC AGAGGCCATT 
CACAACTGGA TCCGTGGGAA TGACAAGGTG CCAGGTGCCT GGACAGAGGC TTGTGGGCAG 
AAGCTGACAT TTTTCAACTC GACACTCAAC ACTTCAGGAC TGTCAACCCA GGGAGAAGCT 
TTACCCATCC CAGGAGCCCA TCGTCCAGGC GTGGTCACCA AAGCAGGACT CATCCTCTTT 
GGGAATGAGC ATAGAATGTT GCTGGTGAAG AACATCCAGC TGGAAGATGG CAAGATGATG 
CCAGCCTCCC AGTTCTTCAA GGGGTCCGCT AGCAGTGACC TGGAGCTGAC AGAGGCGGAG 
TTGGCCACGG CGGAGGCTGT GCGGAGCTCT TGGATGCGAA TCCTGCCGAA CGTCCCAGAG 
GTGGAAGACT CTACAGATTT CTTCAAGTCA GGAGCTGCTT CTGTAGATGT TGTGAGGCTG 
GTGGAGGAAG TGAAGGAGCT GTGTGACGGG CTGGAGTTAG AAAATGAAGA CGTTTACATG 
GCCACCACCT TTAGGGAGTT TATCCAGCTC CTAGTGAGGA AGCTGAGAGG GGAGGACGAC 
GAGAGCGAGT GTGTCATTAA CTACGTGGAA AGGGCAGTGA ACAAGCTGAC TCTCCAAATG 
CCCTACCAGC TCTTCATAGG CGGCGAGTTC GTGGACGCTG AGGGCTCTAA GACCTACAAT 
ACCATCAACC CAACGGATGG AAGTGTCATC TGCCAGGTGT CCCTGGCTCA GGTGAGCGAT 
GTTGACAAGG CAGTGGCAGC AGCAAAGGAA GCCTTCGAGA ACGGACTGTG GGGAAAGATA 
AATGCTCGTG ACCGGGGCCG GCTCCTGTAC AGGTTGGCGG ATGTCATGGA GCAGCACCAG 
GAAGAGCTAG CCACCATTGA GGCCCTGGAT CGAGGTGCCG TCTACACGCT GGCCCTGAAG 
ACGCACGTGG GCATGTCCAT CCAGACCTTC CGGTACTTTG CTGGCTGGTG TGATAAGATC 
CAGGGTGCCA CCATCCCCAT CAACCAGGCT AGACCCAACC GCAACCTGAC CTTGACCAAG 
AAGGAACCTG TTGGGGTCTG TGGCATTGTC ATCCCCTGGA ACTATCCCTT AATGATGCTA 
TCCTGGAAGA CTGCAGCCTG CCTGGCGGCT GGGAACACCG TGGTGATCAA GCCTGCCCAG 
GTGACCCCAC TCACAGCCTT GAAGTTTGCC GAGCTGACAC TGAAGGCTGG CATTCCCAAG 
GGTGTGGTCA ACATCCTCCC AGGATCTGGA TCCCTGGTTG GCCAGAGACT CTCAGACCAC 
CCTGATGTGA GGAAAATAGG GTTCACAGGC TCCACGGAGG TGGGGAAACA CATCATGAAA 
AGCTGTGCTC TGAGCAACGT GAAGAAAGTC TCCCTGGAGC TGGGTGGAAA GTCACCCCTT 
ATCATATTTG CTGACTGCGA CCTCAACAAG GCCGTGCAGA TGGGCATGAG CTCCGTTTTC 
TTCAACAAAG GGGAGAACTG CATTGCGGCA GGCCGGCTCT TTGTGGAGGA GTCCATCCAC 
AACCAGTTTG TGCAGAAAGT GGTGGAGGAG GTGGAGAAGA TGAAAATCGG CAATCCCCTG 
GAGAGGGACA CCAATCATGG CCCGCAGAAC CATGAGGCCC ACCTGAGGAA GCTAGTGGAG 
TATTGCCAAC GTGGTGTGAA GGAAGGGGCC ACACTGGTCT GTGGTGGGAA CCAAGTCCCA 
AGGCCAGGAT TCTTCTTTCA GCCAACCGTC TTCACAGACG TAGAGGACCA CATGTACATC 
GCTAAGGAGG AGTCCTTTGG GCCCATCATG ATCATCTCTC GGTTTGCTGA TGGGGATGTG 
GATGCTGTGC TGTCCCGGGC CAATGCCACA GAATTTGGCC TGGCCTCTGG GGTCTTCACC 
CGCGATATCA ACAAGGCCCT GTATGTCAGT GACAAACTGC AGGCAGGCAC TGTGTTCATC 
AACACGTATA ACAAGACTGA TGTGGCCGCA CCTTTTGGAG GATTCAAGCA ATCTGGATTT 
GGCAAAGACC TGGGAGAGGC AGCCCTGAAT GAGTACCTGC GGATCAAGAC TGTGACTTTT 
GAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071992.1
CDS	163..2871
Translation	MKIAVIGQSLFGQEVYCQLRKEGHEVVGVFTIPDKDGKADPLGLEAEKDGRAVFKFPRWRARGQALPEVVAKYQALGAELNVLPFCSQFIPMEVINAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFTIFWADDGLDTGDLLLQKECEVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGTAPRCPQSEEGATYEGIQKKETAKINWDQPAEAIHNWIRGNDKVPGAWTEACGQKLTFFNSTLNTSGLSTQGEALPIPGAHRPGVVTKAGLILFGNEHRMLLVKNIQLEDGKMMPASQFFKGSASSDLELTEAELATAEAVRSSWMRILPNVPEVEDSTDFFKSGAASVDVVRLVEEVKELCDGLELENEDVYMATTFREFIQLLVRKLRGEDDESECVINYVERAVNKLTLQMPYQLFIGGEFVDAEGSKTYNTINPTDGSVICQVSLAQVSDVDKAVAAAKEAFENGLWGKINARDRGRLLYRLADVMEQHQEELATIEALDRGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGATIPINQARPNRNLTLTKKEPVGVCGIVIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPKGVVNILPGSGSLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCALSNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVFFNKGENCIAAGRLFVEESIHNQFVQKVVEEVEKMKIGNPLERDTNHGPQNHEAHLRKLVEYCQRGVKEGATLVCGGNQVPRPGFFFQPTVFTDVEDHMYIAKEESFGPIMIISRFADGDVDAVLSRANATEFGLASGVFTRDINKALYVSDKLQAGTVFINTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRIKTVTFEY

Target ORF information:

RefSeq Version	NM_022547.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aldehyde dehydrogenase 1 family, member L1 (Aldh1l1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022547.1

ORF Insert Sequence:

ATGAAGATTG CAGTAATCGG ACAGAGCCTG TTTGGCCAGG AGGTTTACTG CCAGCTGAGG 
AAGGAGGGCC ACGAGGTGGT GGGTGTGTTC ACAATCCCAG ACAAGGATGG GAAAGCAGAC 
CCCCTGGGCC TGGAGGCTGA GAAGGATGGC CGTGCCGTGT TCAAGTTTCC TCGGTGGCGT 
GCTCGGGGAC AGGCTCTGCC CGAGGTGGTG GCCAAATACC AGGCTCTGGG TGCAGAGCTC 
AATGTGCTTC CCTTCTGCAG CCAGTTCATA CCCATGGAGG TCATTAATGC CCCCAGGCAC 
GGCTCCATCA TCTACCATCC ATCCCTGCTA CCCAGGCATC GAGGGGCTTC GGCCATCAAC 
TGGACCCTCA TTCATGGAGA TAAGAAAGGA GGCTTCACCA TCTTCTGGGC TGATGATGGC 
CTGGACACTG GTGACCTTCT GCTGCAGAAG GAGTGTGAGG TGCTTCCAGA TGACACCGTG 
AGCACGCTGT ACAATCGTTT CCTCTTCCCA GAGGGCATCA AAGGGATGGT TCAGGCTGTG 
CGACTGATCG CAGAGGGTAC AGCCCCCCGC TGCCCCCAGT CTGAGGAAGG AGCCACCTAT 
GAGGGCATTC AGAAGAAGGA GACAGCCAAG ATCAATTGGG ACCAGCCAGC AGAGGCCATT 
CACAACTGGA TCCGTGGGAA TGACAAGGTG CCAGGTGCCT GGACAGAGGC TTGTGGGCAG 
AAGCTGACAT TTTTCAACTC GACACTCAAC ACTTCAGGAC TGTCAACCCA GGGAGAAGCT 
TTACCCATCC CAGGAGCCCA TCGTCCAGGC GTGGTCACCA AAGCAGGACT CATCCTCTTT 
GGGAATGAGC ATAGAATGTT GCTGGTGAAG AACATCCAGC TGGAAGATGG CAAGATGATG 
CCAGCCTCCC AGTTCTTCAA GGGGTCCGCT AGCAGTGACC TGGAGCTGAC AGAGGCGGAG 
TTGGCCACGG CGGAGGCTGT GCGGAGCTCT TGGATGCGAA TCCTGCCGAA CGTCCCAGAG 
GTGGAAGACT CTACAGATTT CTTCAAGTCA GGAGCTGCTT CTGTAGATGT TGTGAGGCTG 
GTGGAGGAAG TGAAGGAGCT GTGTGACGGG CTGGAGTTAG AAAATGAAGA CGTTTACATG 
GCCACCACCT TTAGGGAGTT TATCCAGCTC CTAGTGAGGA AGCTGAGAGG GGAGGACGAC 
GAGAGCGAGT GTGTCATTAA CTACGTGGAA AGGGCAGTGA ACAAGCTGAC TCTCCAAATG 
CCCTACCAGC TCTTCATAGG CGGCGAGTTC GTGGACGCTG AGGGCTCTAA GACCTACAAT 
ACCATCAACC CAACGGATGG AAGTGTCATC TGCCAGGTGT CCCTGGCTCA GGTGAGCGAT 
GTTGACAAGG CAGTGGCAGC AGCAAAGGAA GCCTTCGAGA ACGGACTGTG GGGAAAGATA 
AATGCTCGTG ACCGGGGCCG GCTCCTGTAC AGGTTGGCGG ATGTCATGGA GCAGCACCAG 
GAAGAGCTAG CCACCATTGA GGCCCTGGAT CGAGGTGCCG TCTACACGCT GGCCCTGAAG 
ACGCACGTGG GCATGTCCAT CCAGACCTTC CGGTACTTTG CTGGCTGGTG TGATAAGATC 
CAGGGTGCCA CCATCCCCAT CAACCAGGCT AGACCCAACC GCAACCTGAC CTTGACCAAG 
AAGGAACCTG TTGGGGTCTG TGGCATTGTC ATCCCCTGGA ACTATCCCTT AATGATGCTA 
TCCTGGAAGA CTGCAGCCTG CCTGGCGGCT GGGAACACCG TGGTGATCAA GCCTGCCCAG 
GTGACCCCAC TCACAGCCTT GAAGTTTGCC GAGCTGACAC TGAAGGCTGG CATTCCCAAG 
GGTGTGGTCA ACATCCTCCC AGGATCTGGA TCCCTGGTTG GCCAGAGACT CTCAGACCAC 
CCTGATGTGA GGAAAATAGG GTTCACAGGC TCCACGGAGG TGGGGAAACA CATCATGAAA 
AGCTGTGCTC TGAGCAACGT GAAGAAAGTC TCCCTGGAGC TGGGTGGAAA GTCACCCCTT 
ATCATATTTG CTGACTGCGA CCTCAACAAG GCCGTGCAGA TGGGCATGAG CTCCGTTTTC 
TTCAACAAAG GGGAGAACTG CATTGCGGCA GGCCGGCTCT TTGTGGAGGA GTCCATCCAC 
AACCAGTTTG TGCAGAAAGT GGTGGAGGAG GTGGAGAAGA TGAAAATCGG CAATCCCCTG 
GAGAGGGACA CCAATCATGG CCCGCAGAAC CATGAGGCCC ACCTGAGGAA GCTAGTGGAG 
TATTGCCAAC GTGGTGTGAA GGAAGGGGCC ACACTGGTCT GTGGTGGGAA CCAAGTCCCA 
AGGCCAGGAT TCTTCTTTCA GCCAACCGTC TTCACAGACG TAGAGGACCA CATGTACATC 
GCTAAGGAGG AGTCCTTTGG GCCCATCATG ATCATCTCTC GGTTTGCTGA TGGGGATGTG 
GATGCTGTGC TGTCCCGGGC CAATGCCACA GAATTTGGCC TGGCCTCTGG GGTCTTCACC 
CGCGATATCA ACAAGGCCCT GTATGTCAGT GACAAACTGC AGGCAGGCAC TGTGTTCATC 
AACACGTATA ACAAGACTGA TGTGGCCGCA CCTTTTGGAG GATTCAAGCA ATCTGGATTT 
GGCAAAGACC TGGGAGAGGC AGCCCTGAAT GAGTACCTGC GGATCAAGAC TGTGACTTTT 
GAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase.
Chemico-biological interactions191(1-3)129-36(2011 May)
Strickland KC,Krupenko NI,Dubard ME,Hu CJ,Tsybovsky Y,Krupenko SA

Polymorphisms in 1-carbon metabolism, epigenetics and folate-related pathologies.
Journal of nutrigenetics and nutrigenomics4(5)293-305(2011)
Stover PJ

ALDH1L2 is the mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase.
The Journal of biological chemistry285(30)23056-63(2010 Jul)
Krupenko NI,Dubard ME,Strickland KC,Moxley KM,Oleinik NV,Krupenko SA

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Rat liver 10-formyltetrahydrofolate dehydrogenase, carbamoyl phosphate synthetase 1 and betaine homocysteine S-methytransferase were co-purified on Kunitz-type soybean trypsin inhibitor-coupled sepharose CL-4B.
Journal of biochemistry and molecular biology40(4)604-9(2007 Jul)
Kim HS,Kim JM,Roh KB,Lee HH,Kim SJ,Shin YH,Lee BL