Pmpcb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pmpcb
Entrez Gene ID	64198
Full Name	peptidase, mitochondrial processing beta subunit
Synonyms	Mppb
General protein information	Preferred Names peptidase, mitochondrial processing beta subunit Names mitochondrial-processing peptidase subunit beta P-52 beta-MPP mitochondrial processing peptidase beta subunit peptidase (mitochondrial processing) beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q11
Summary	beta subunit of mitochondrial processing peptidase, which catalyzes cleavage of the amino terminal leader peptide of nuclear encoded mitochondrial proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022395.2	NP_071790.1	mitochondrial-processing peptidase subunit beta precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pmpcb	NP_071790.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000935	XP_309120.4
Neurospora crassa	NCU02549	XP_965680.1
Arabidopsis thaliana (thale cress)	MPPBETA	NP_186858.1
Caenorhabditis elegans (roundworm)	mppb-1	NP_501576.2
Homo sapiens (human)	PMPCB	NP_004270.2
Pan troglodytes (chimpanzee)	PMPCB	XP_001160333.2
Mus musculus (house mouse)	Pmpcb	NP_082707.1
Gallus gallus (chicken)	PMPCB	NP_001186401.1
Danio rerio (zebrafish)	pmpcb	NP_001012514.1
Kluyveromyces lactis	KLLA0D18095g	XP_453861.1
Eremothecium gossypii	AGOS_AGL138C	NP_986529.1
Schizosaccharomyces pombe (fission yeast)	qcr1	NP_595827.1
Magnaporthe oryzae (rice blast fungus)	MGG_03600	XP_003716305.1
Canis lupus familiaris (dog)	PMPCB	XP_005631033.1
Saccharomyces cerevisiae (baker's yeast)	MAS1	NP_013264.1
Oryza sativa (rice)	Os03g0212700	NP_001049357.1
Xenopus tropicalis (tropical clawed frog)	pmpcb	NP_001039103.1
Bos taurus (cattle)	PMPCB	NP_001029785.1
Drosophila melanogaster (fruit fly)	CG3731	NP_731954.1

Gene Ontology
Bibliography

Related articles in PubMed

Processing of mitochondrial presequences.
Mossmann D, Meisinger C, V?gtle FN
Biochimica et biophysica acta1819(9-10)1098-106(2012 Sep-Oct)

Mitochondrial processing peptidase regulates PINK1 processing, import and Parkin recruitment.
Greene AW, Grenier K, Aguileta MA, Muise S, Farazifard R, Haque ME, McBride HM, Park DS, Fon EA
EMBO reports13(4)378-85(2012 Apr)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Proteomic analysis of the mouse liver mitochondrial inner membrane.
Da Cruz S, Xenarios I, Langridge J, Vilbois F, Parone PA, Martinou JC
The Journal of biological chemistry278(42)41566-71(2003 Oct)

Determination of the cleavage site of the presequence by mitochondrial processing peptidase on the substrate binding scaffold and the multiple subsites inside a molecular cavity.
Kitada S, Yamasaki E, Kojima K, Ito A
The Journal of biological chemistry278(3)1879-85(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pmpcb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pmpcb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12315	NM_022395.2 Latest version!	Rattus norvegicus peptidase, mitochondrial processing subunit beta (Pmpcb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pmpcb ( NM_022395.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071790.1 Rattus norvegicus peptidase, mitochondrial processing subunit beta (Pmpcb), mRNA.

Price & Availability↑

CloneID	ORa12315
Clone ID Related Accession (Same CDS sequence)	NM_022395.2
Accession Version	NM_022395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-10
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial-processing peptidase subunit beta precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L12965.1. On Aug 31, 2012 this sequence version replaced NM_022395.1. ##Evidence-Data-START## Transcript exon combination :: L12965.1, BC078826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGG CGGCTGTATC CCGGACGTTG TTGCCGGTGG CCGGACGGCG CCTGTGGGGA 
TTTACGCGAA GGCTTCCGCT CCGCGCCGCC GCTGCTCAGC CATTGTACTT TGGAGGGGAC 
CGACTCAGAA GTACACAGGC TGCTCCACAA GTTGTTCTGA ATGTTCCTGA GACACAAGTC 
ACTTGTTTGG AAAATGGACT CAGGGTAGCT TCTGAAAACT CTGGGATCTC AACATGCACC 
GTTGGGCTGT GGATCGATGC TGGCAGTCGG TATGAGAATG AAAAGAACAA TGGCACCGCT 
CACTTTCTGG AGCACATGGC TTTCAAGGGC ACTAAAAAGA GGTCCCAGTT AGACCTTGAA 
CTTGAGATTG AGAACATGGG TGCCCATCTC AATGCCTATA CCTCCAGAGA GCAGACTGTC 
TACTACGCCA AAGCCTTCTC CAAAGATTTG CCAAGAGCTG TAGAAATTCT TGCTGACATA 
ATTCAGAACA GTACATTGGG AGAGGCAGAG ATTGAACGTG AGCGTGGAGT CATCCTCAGA 
GAAATGCAGG AAGTTGAGAC CAACTTGCAA GAAGTTGTCT TTGATTATCT GCACGCCACA 
GCCTATCAAA ACACCGCGCT CGGACGGACA ATTCTGGGAC CAACCGAGAA CATCAAATCT 
ATAAGCCGTA AGGACTTAGT GGACTACATA ACCACACATT ATAAGGGACC AAGAATCGTG 
CTGGCTGCTG CTGGAGGTGT TTGCCATAAC GAACTGCTGG AGTTAGCGAA GTTTCATTTT 
GGTGACTCTT TGTGCGCACA CAAAGGAGAT GTACCAGCTC TGCCTCCCTG TAAATTCACT 
GGAAGCGAGA TTCGGGTGAG GGATGACAAG ATGCCACTGG CGCACCTCGC AGTGGCTATT 
GAAGCAGTTG GTTGGACGCA CCCAGACACG ATCCGTCTCA TGGTCGCAAA CACACTGATA 
GGCAACTGGG ACCGCTCTTT CGGAGGAGGA ATGAATTTAT CGAGCAAGCT GGCCCAGCTC 
ACGTGCCATG GCAATCTCTG CCACAGCTTC CAGTCCTTCA ACACCTCCTA CACAGACACA 
GGATTGTGGG GGCTCTATAT GGTTTGTGAA CAAGCCACAG TTGCTGACAT GCTGCACGCT 
GTACAAAAGG AATGGATGCG TCTGTGCACA GCTGTTAGTG AGAGTGAGGT CGCACGCGCC 
AAAAACCTTC TCAAGACAAA CATGCTGCTG CAGCTTGATG GGTCAACTCC GATCTGTGAA 
GACATCGGTA GGCAGATGCT ATGCTACAAC AGGAGAATCC CCATCCCTGA GCTTGAGGCC 
AGGATCGATG CTGTGGACGC GGAGATGGTT CGAGAAGTGT GCACCAAGTA CATTTATGGC 
AAAAGCCCGG CCATTGCTGC TCTCGGTCCT ATTGAGCGCT TACCAGATTT TAACCAGATT 
TGTAGTAACA TGCGCTGGAC TCGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071790.1
CDS	17..1486
Translation	MAAAAVSRTLLPVAGRRLWGFTRRLPLRAAAAQPLYFGGDRLRSTQAAPQVVLNVPETQVTCLENGLRVASENSGISTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSISRKDLVDYITTHYKGPRIVLAAAGGVCHNELLELAKFHFGDSLCAHKGDVPALPPCKFTGSEIRVRDDKMPLAHLAVAIEAVGWTHPDTIRLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCHSFQSFNTSYTDTGLWGLYMVCEQATVADMLHAVQKEWMRLCTAVSESEVARAKNLLKTNMLLQLDGSTPICEDIGRQMLCYNRRIPIPELEARIDAVDAEMVREVCTKYIYGKSPAIAALGPIERLPDFNQICSNMRWTRD

Target ORF information:

RefSeq Version	NM_022395.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peptidase, mitochondrial processing subunit beta (Pmpcb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022395.2

ORF Insert Sequence:

ATGGCGGCGG CGGCTGTATC CCGGACGTTG TTGCCGGTGG CCGGACGGCG CCTGTGGGGA 
TTTACGCGAA GGCTTCCGCT CCGCGCCGCC GCTGCTCAGC CATTGTACTT TGGAGGGGAC 
CGACTCAGAA GTACACAGGC TGCTCCACAA GTTGTTCTGA ATGTTCCTGA GACACAAGTC 
ACTTGTTTGG AAAATGGACT CAGGGTAGCT TCTGAAAACT CTGGGATCTC AACATGCACC 
GTTGGGCTGT GGATCGATGC TGGCAGTCGG TATGAGAATG AAAAGAACAA TGGCACCGCT 
CACTTTCTGG AGCACATGGC TTTCAAGGGC ACTAAAAAGA GGTCCCAGTT AGACCTTGAA 
CTTGAGATTG AGAACATGGG TGCCCATCTC AATGCCTATA CCTCCAGAGA GCAGACTGTC 
TACTACGCCA AAGCCTTCTC CAAAGATTTG CCAAGAGCTG TAGAAATTCT TGCTGACATA 
ATTCAGAACA GTACATTGGG AGAGGCAGAG ATTGAACGTG AGCGTGGAGT CATCCTCAGA 
GAAATGCAGG AAGTTGAGAC CAACTTGCAA GAAGTTGTCT TTGATTATCT GCACGCCACA 
GCCTATCAAA ACACCGCGCT CGGACGGACA ATTCTGGGAC CAACCGAGAA CATCAAATCT 
ATAAGCCGTA AGGACTTAGT GGACTACATA ACCACACATT ATAAGGGACC AAGAATCGTG 
CTGGCTGCTG CTGGAGGTGT TTGCCATAAC GAACTGCTGG AGTTAGCGAA GTTTCATTTT 
GGTGACTCTT TGTGCGCACA CAAAGGAGAT GTACCAGCTC TGCCTCCCTG TAAATTCACT 
GGAAGCGAGA TTCGGGTGAG GGATGACAAG ATGCCACTGG CGCACCTCGC AGTGGCTATT 
GAAGCAGTTG GTTGGACGCA CCCAGACACG ATCCGTCTCA TGGTCGCAAA CACACTGATA 
GGCAACTGGG ACCGCTCTTT CGGAGGAGGA ATGAATTTAT CGAGCAAGCT GGCCCAGCTC 
ACGTGCCATG GCAATCTCTG CCACAGCTTC CAGTCCTTCA ACACCTCCTA CACAGACACA 
GGATTGTGGG GGCTCTATAT GGTTTGTGAA CAAGCCACAG TTGCTGACAT GCTGCACGCT 
GTACAAAAGG AATGGATGCG TCTGTGCACA GCTGTTAGTG AGAGTGAGGT CGCACGCGCC 
AAAAACCTTC TCAAGACAAA CATGCTGCTG CAGCTTGATG GGTCAACTCC GATCTGTGAA 
GACATCGGTA GGCAGATGCT ATGCTACAAC AGGAGAATCC CCATCCCTGA GCTTGAGGCC 
AGGATCGATG CTGTGGACGC GGAGATGGTT CGAGAAGTGT GCACCAAGTA CATTTATGGC 
AAAAGCCCGG CCATTGCTGC TCTCGGTCCT ATTGAGCGCT TACCAGATTT TAACCAGATT 
TGTAGTAACA TGCGCTGGAC TCGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Processing of mitochondrial presequences.
Biochimica et biophysica acta1819(9-10)1098-106(2012 Sep-Oct)
Mossmann D,Meisinger C,V?gtle FN

Mitochondrial processing peptidase regulates PINK1 processing, import and Parkin recruitment.
EMBO reports13(4)378-85(2012 Apr)
Greene AW,Grenier K,Aguileta MA,Muise S,Farazifard R,Haque ME,McBride HM,Park DS,Fon EA

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Proteomic analysis of the mouse liver mitochondrial inner membrane.
The Journal of biological chemistry278(42)41566-71(2003 Oct)
Da Cruz S,Xenarios I,Langridge J,Vilbois F,Parone PA,Martinou JC

Determination of the cleavage site of the presequence by mitochondrial processing peptidase on the substrate binding scaffold and the multiple subsites inside a molecular cavity.
The Journal of biological chemistry278(3)1879-85(2003 Jan)
Kitada S,Yamasaki E,Kojima K,Ito A