Npvf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npvf
Entrez Gene ID	60570
Full Name	neuropeptide VF precursor
Synonyms	Rfrp
General protein information	Preferred Names neuropeptide VF precursor Names pro-FMRFamide-related neuropeptide VF FMRFamide-related peptides RFamide-related peptide
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q24
Summary	binds neuropeptide receptor FF1; blocks morphine induced analgesia in acute and inflammatory pain models; may play a role in nociception and anti-opiod response [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_023952.1	NP_076442.1	pro-FMRFamide-related neuropeptide VF precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	NPVF	NP_071433.3
	NPVF	NP_001028287.1
Canis lupus familiaris (dog)	NPVF	XP_003431970.1
Mus musculus (house mouse)	Npvf	NP_068692.1
Pan troglodytes (chimpanzee)	NPVF	XP_001160762.1
Bos taurus (cattle)	NPVF	NP_776593.1
Gallus gallus (chicken)	NPVF	NP_989694.1
Rattus norvegicus (Norway rat)	Npvf	NP_076442.1

Gene Ontology
Bibliography

Related articles in PubMed

Physiological roles of gonadotropin-inhibitory hormone signaling in the control of mammalian reproductive axis: studies in the NPFF1 receptor null mouse.
Le?n S, Garc?a-Galiano D, Ruiz-Pino F, Barroso A, Manfredi-Lozano M, Romero-Ruiz A, Roa J, V?zquez MJ, Gaytan F, Blomenrohr M, van Duin M, Pinilla L, Tena-Sempere M
Endocrinology155(8)2953-65(2014 Aug)

Glucocorticoid receptor-mediated regulation of Rfrp (GnIH) and Gpr147 (GnIH-R) synthesis in immortalized hypothalamic neurons.
Gojska NM, Belsham DD
Molecular and cellular endocrinology384(1-2)23-31(2014 Mar)

Developmental changes in the mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide-related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus.
Iwasa T, Matsuzaki T, Murakami M, Kinouchi R, Osugi T, Gereltsetseg G, Yoshida S, Irahara M, Tsutsui K
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience30(1)31-7(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hypothalamic expression of Rfrp and Rfrp1r receptor in rats peaks during the pubertal period in males (postnatal day (PND)-35 to PND-42) but not in females
Title: Physiological roles of gonadotropin-inhibitory hormone signaling in the control of mammalian reproductive axis: studies in the NPFF1 receptor null mouse.

Rfrp (GnIH)-expressing neurons serve as potential upstream mediators of stress-induced effects through Gpr147 (GnIH-R)-dependent mechanisms.
Title: Glucocorticoid receptor-mediated regulation of Rfrp (GnIH) and Gpr147 (GnIH-R) synthesis in immortalized hypothalamic neurons.

The hypothalamic RFRP system changes during development. Ovarian sex steroid estradiol-17beta (E2) may stimulate mRNA expression of RFRP in the prepubertal period when the basal E2 concentration is low.
Title: Developmental changes in the mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide-related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus.

RFRP-3 inhibits secretion of LH and FSH inmammals.
Title: Characterization of the inhibitory roles of RFRP3, the mammalian ortholog of GnIH, in the control of gonadotropin secretion in the rat: in vivo and in vitro studies.

Developmental and steroidogenic effects on the gene expression of RFamide related peptides and their receptor in the rat brain and pituitary gland.
Title: Developmental and steroidogenic effects on the gene expression of RFamide related peptides and their receptor in the rat brain and pituitary gland.

The following Npvf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npvf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06626	NM_023952.1 Latest version!	Rattus norvegicus neuropeptide VF precursor (Npvf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npvf ( NM_023952.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_076442.1 Rattus norvegicus neuropeptide VF precursor (Npvf), mRNA.

Price & Availability↑

CloneID	ORa06626
Clone ID Related Accession (Same CDS sequence)	NM_023952.1
Accession Version	NM_023952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Rattus norvegicus(Norway rat)
Product	pro-FMRFamide-related neuropeptide VF precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB040288.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB040288.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAAATTA TTTCATCAAA GCGATTCATT TTATTGACTT TAGCAACTTC AAGCTTCTTA 
ACTTCAAACA CCCTTTGTTC AGATGAATTA ATGATGCCCC ATTTTCACAG CAAAGAAGGT 
TATGGAAAAT ATTACCAGCT GAGAGGAATC CCAAAAGGGG TAAAGGAAAG AAGTGTCACT 
TTTCAAGAAC TCAAAGATTG GGGGGCAAAG AAAGATATTA AGATGAGTCC AGCCCCTGCC 
AACAAAGTGC CCCACTCAGC AGCCAACCTT CCCCTGAGGT TTGGGAGGAA CATAGAAGAC 
AGAAGAAGCC CCAGGGCACG GGCCAACATG GAGGCAGGGA CCATGAGCCA TTTTCCCAGC 
CTGCCCCAAA GGTTTGGGAG AACAACAGCC AGACGCATCA CCAAGACACT GGCTGGTTTG 
CCCCAGAAAT CCCTGCACTC CCTGGCCTCC AGTGAATTGC TCTATGCCAT GACCCGCCAG 
CATCAAGAAA TTCAGAGTCC TGGTCAAGAG CAACCTAGGA AACGGGTGTT CACGGAAACA 
GATGATGCAG AAAGGAAACA AGAAAAAATA GGAAACCTCC AGCCAGTCCT TCAAGGGGCT 
ATGAAGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076442.1
CDS	1..612
Translation	MEIISSKRFILLTLATSSFLTSNTLCSDELMMPHFHSKEGYGKYYQLRGIPKGVKERSVTFQELKDWGAKKDIKMSPAPANKVPHSAANLPLRFGRNIEDRRSPRARANMEAGTMSHFPSLPQRFGRTTARRITKTLAGLPQKSLHSLASSELLYAMTRQHQEIQSPGQEQPRKRVFTETDDAERKQEKIGNLQPVLQGAMKL

Target ORF information:

RefSeq Version	NM_023952.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuropeptide VF precursor (Npvf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023952.1

ORF Insert Sequence:

ATGGAAATTA TTTCATCAAA GCGATTCATT TTATTGACTT TAGCAACTTC AAGCTTCTTA 
ACTTCAAACA CCCTTTGTTC AGATGAATTA ATGATGCCCC ATTTTCACAG CAAAGAAGGT 
TATGGAAAAT ATTACCAGCT GAGAGGAATC CCAAAAGGGG TAAAGGAAAG AAGTGTCACT 
TTTCAAGAAC TCAAAGATTG GGGGGCAAAG AAAGATATTA AGATGAGTCC AGCCCCTGCC 
AACAAAGTGC CCCACTCAGC AGCCAACCTT CCCCTGAGGT TTGGGAGGAA CATAGAAGAC 
AGAAGAAGCC CCAGGGCACG GGCCAACATG GAGGCAGGGA CCATGAGCCA TTTTCCCAGC 
CTGCCCCAAA GGTTTGGGAG AACAACAGCC AGACGCATCA CCAAGACACT GGCTGGTTTG 
CCCCAGAAAT CCCTGCACTC CCTGGCCTCC AGTGAATTGC TCTATGCCAT GACCCGCCAG 
CATCAAGAAA TTCAGAGTCC TGGTCAAGAG CAACCTAGGA AACGGGTGTT CACGGAAACA 
GATGATGCAG AAAGGAAACA AGAAAAAATA GGAAACCTCC AGCCAGTCCT TCAAGGGGCT 
ATGAAGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Physiological roles of gonadotropin-inhibitory hormone signaling in the control of mammalian reproductive axis: studies in the NPFF1 receptor null mouse.
Endocrinology155(8)2953-65(2014 Aug)
Le?n S,Garc?a-Galiano D,Ruiz-Pino F,Barroso A,Manfredi-Lozano M,Romero-Ruiz A,Roa J,V?zquez MJ,Gaytan F,Blomenrohr M,van Duin M,Pinilla L,Tena-Sempere M

Glucocorticoid receptor-mediated regulation of Rfrp (GnIH) and Gpr147 (GnIH-R) synthesis in immortalized hypothalamic neurons.
Molecular and cellular endocrinology384(1-2)23-31(2014 Mar)
Gojska NM,Belsham DD

Developmental changes in the mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide-related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience30(1)31-7(2012 Feb)
Iwasa T,Matsuzaki T,Murakami M,Kinouchi R,Osugi T,Gereltsetseg G,Yoshida S,Irahara M,Tsutsui K