Mid1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mid1
Entrez Gene ID	54252
Full Name	midline 1
Synonyms	Fxy,Midline1
General protein information	Preferred Names midline 1 Names E3 ubiquitin-protein ligase Midline-1 Finger on X and Y (in rat only on X) RING finger protein Midline-1 RING-type E3 ubiquitin transferase Midline-1 midline 1 (Opitz/BBB syndrome) tripartite motif-containing protein 18
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq13
Summary	human homolog forms homodimers which associate with microtubules in the cytoplasm and mutations in gene are associated with Opitz syndrome [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017602143.1	XP_017457632.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_006256825.3	XP_006256887.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_017602142.1	XP_017457631.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
NM_022927.1	NP_075216.1	E3 ubiquitin-protein ligase Midline-1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mid1	NP_034927.2
Homo sapiens (human)	MID1	NP_150632.1
Rattus norvegicus (Norway rat)	Mid1	NP_075216.1
Danio rerio (zebrafish)	mid1	XP_002663478.1
Xenopus tropicalis (tropical clawed frog)	mid1	NP_001006876.1
Bos taurus (cattle)	MID1	NP_001179751.1
Gallus gallus (chicken)	MID1	NP_989460.1
	MID1	XP_001093390.1
Canis lupus familiaris (dog)	MID1	XP_548857.2

Gene Ontology
Bibliography

Related articles in PubMed

Systematic analysis of dimeric E3-RING interactions reveals increased combinatorial complexity in human ubiquitination networks.
Woodsmith J, Jenn RC, Sanderson CM
Molecular & cellular proteomics : MCP11(7)M111.016162(2012 Jul)

TRIM27 negatively regulates NOD2 by ubiquitination and proteasomal degradation.
Zurek B, Schoultz I, Neerincx A, Napolitano LM, Birkner K, Bennek E, Sellge G, Lerm M, Meroni G, S?derholm JD, Kufer TA
PloS one7(7)e41255(2012)

Active transport of the ubiquitin ligase MID1 along the microtubules is regulated by protein phosphatase 2A.
Aranda-Orgill?s B, Aigner J, Kunath M, Lurz R, Schneider R, Schweiger S
PloS one3(10)e3507(2008)

Alternative polyadenylation signals and promoters act in concert to control tissue-specific expression of the Opitz Syndrome gene MID1.
Winter J, Kunath M, Roepcke S, Krause S, Schneider R, Schweiger S
BMC molecular biology8105(2007 Nov)

Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3.
Prickett TD, Brautigan DL
Molecular and cellular biology27(12)4217-27(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mid1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mid1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13887	NM_022927.1 Latest version!	Rattus norvegicus midline 1 (Mid1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa39661	XM_017602142.1 Latest version!	Rattus norvegicus midline 1 (Mid1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa39661	XM_017602143.1 Latest version!	Rattus norvegicus midline 1 (Mid1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa39661	XM_006256825.3 Latest version!	Rattus norvegicus midline 1 (Mid1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mid1 ( NM_022927.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_075216.1 Rattus norvegicus midline 1 (Mid1), mRNA.

Price & Availability↑

CloneID	ORa13887
Clone ID Related Accession (Same CDS sequence)	NM_022927.1
Accession Version	NM_022927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF186461.1. ##Evidence-Data-START## Transcript exon combination :: AF186461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMEA2689600 [ECO:0000348] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
CTCCTGCTTC CCTGTGCACA TAGCCTCTGT TTCAACTGTG CCCACCGCAT CCTGGTCTCT 
CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCTTACAA 
AACATCATTG ACAGGTTTCA GAAAGCATCC GTGAGTGGGC CCAATTCTCC CAGTGAGACC 
CGCAGGGAGC GGGCCTTTGA CGCCAACACC ATGTCTTCTG CCGAGAAAGT TCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACCT GTGTTACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTAA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAATTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAGGTCA ATGCATCCCG TCAAGAAGCC 
AAGTTGACAG AGGAATGTGA TCTTCTCATT GAAATTATTC AGGAAAGAAG ACAAATTATT 
GGAACAAAGA TTAAAGAAGG CAAGGTGATG AGGCTCCGCA AATTAGCTCA GCAGATTGCA 
AACTGTAAAC AATGCATTGA AAGGTCTGCA TCACTCATTT CCCAAGCAGA ACACTCACTG 
AAGGAGAATG ACCATGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTTTGGA TTACCTAACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACT 
GTCCACTGGA CTTCGGATGA TGAGTTCAGT GTGGTCTCCT ATGAACTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT CGTTAGTCTT TGTAACTCGG CAGATAGCTG GATGATTGTG 
CCCAACATCA AGCAGAACCA CTATACTGTG CATGGCCTAC AAAGTGGCAC CAAGTATATC 
TTCATGGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGCTGAAG 
ACAAACAGTC AGCCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT CAAGGTGTCC 
CATGACAACC TGACTGTTGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACTA GCCAAGGGAG CTATGGCGTG GCTGGAAATG TGTTTATTGA CAGTGGCCGT 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCGTACAAA 
TCAGCTCCAA AACATGAATG GATTGGGAAG AATTCTGCTT CCTGGGCCCT TTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACATAACAGC AAGGAAATAC CCATTGAACC AGCCCCCCAC 
CCCCGGCGTG TAGGCATCCT GCTGGACTAT GATAATGGCT CAATCGCTTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA TACCTTCGAT GTGGCGCTTG CGCAGCCTGT GTGCCCCACC 
TTTACTGTGT GGAACAAGTG TTTGACGATT ATAACTGGTC TTCCCATCCC AGACCATTTG 
GACTGTACAG AACAGCTACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075216.1
CDS	233..2236
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNEPVESINAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQERRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHPRRVGILLDYDNGSIAFYDALNSIHLYTFDVALAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_022927.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus midline 1 (Mid1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022927.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
CTCCTGCTTC CCTGTGCACA TAGCCTCTGT TTCAACTGTG CCCACCGCAT CCTGGTCTCT 
CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCTTACAA 
AACATCATTG ACAGGTTTCA GAAAGCATCC GTGAGTGGGC CCAATTCTCC CAGTGAGACC 
CGCAGGGAGC GGGCCTTTGA CGCCAACACC ATGTCTTCTG CCGAGAAAGT TCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACCT GTGTTACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTAA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAATTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAGGTCA ATGCATCCCG TCAAGAAGCC 
AAGTTGACAG AGGAATGTGA TCTTCTCATT GAAATTATTC AGGAAAGAAG ACAAATTATT 
GGAACAAAGA TTAAAGAAGG CAAGGTGATG AGGCTCCGCA AATTAGCTCA GCAGATTGCA 
AACTGTAAAC AATGCATTGA AAGGTCTGCA TCACTCATTT CCCAAGCAGA ACACTCACTG 
AAGGAGAATG ACCATGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTTTGGA TTACCTAACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACT 
GTCCACTGGA CTTCGGATGA TGAGTTCAGT GTGGTCTCCT ATGAACTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT CGTTAGTCTT TGTAACTCGG CAGATAGCTG GATGATTGTG 
CCCAACATCA AGCAGAACCA CTATACTGTG CATGGCCTAC AAAGTGGCAC CAAGTATATC 
TTCATGGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGCTGAAG 
ACAAACAGTC AGCCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT CAAGGTGTCC 
CATGACAACC TGACTGTTGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACTA GCCAAGGGAG CTATGGCGTG GCTGGAAATG TGTTTATTGA CAGTGGCCGT 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCGTACAAA 
TCAGCTCCAA AACATGAATG GATTGGGAAG AATTCTGCTT CCTGGGCCCT TTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACATAACAGC AAGGAAATAC CCATTGAACC AGCCCCCCAC 
CCCCGGCGTG TAGGCATCCT GCTGGACTAT GATAATGGCT CAATCGCTTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA TACCTTCGAT GTGGCGCTTG CGCAGCCTGT GTGCCCCACC 
TTTACTGTGT GGAACAAGTG TTTGACGATT ATAACTGGTC TTCCCATCCC AGACCATTTG 
GACTGTACAG AACAGCTACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid1 ( XM_017602143.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457632.1 Rattus norvegicus midline 1 (Mid1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa39661
Clone ID Related Accession (Same CDS sequence)	XM_017602143.1 , XM_006256825.3 , XM_017602142.1
Accession Version	XM_017602143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
CTCCTGCTTC CCTGTGCACA TAGCCTCTGT TTCAACTGTG CCCACCGCAT CCTGGTCTCT 
CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCTTACAA 
AACATCATTG ACAGGTTTCA GAAAGCATCC GTGAGTGGGC CCAATTCTCC CAGTGAGACC 
CGCAGGGAGC GGGCCTTTGA CGCCAACACC ATGTCTTCTG CCGAGAAAGT TCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACCT GTGTTACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATCCCGGA CTCGCATATC CGGGGGCTAA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAATTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAGGTCA ATGCATCCCG TCAAGAAGCC 
AAGTTGACAG AGGAATGTGA TCTTCTCATT GAAATTATTC AGGAAAGAAG ACAAATTATT 
GGAACAAAGA TTAAAGAAGG CAAGGTGATG AGGCTCCGCA AATTAGCTCA GCAGATTGCA 
AACTGTAAAC AATGCATTGA AAGGTCTGCA TCACTCATTT CCCAAGCAGA ACACTCACTG 
AAGGAGAATG ACCATGCCCG TTTTCTACAG ACAGCAAAGA ACATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTTTGGA TTACCTAACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACT 
GTCCACTGGA CTTCGGATGA TGAGTTCAGT GTGGTCTCCT ATGAACTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT CGTTAGTCTT TGTAACTCGG CAGATAGCTG GATGATTGTG 
CCCAACATCA AGCAGAACCA CTATACTGTG CATGGCCTAC AAAGTGGCAC CAAGTATATC 
TTCATGGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGCTGAAG 
ACAAACAGTC AGCCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT CAAGGTGTCC 
CATGACAACC TGACTGTTGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACTA GCCAAGGGAG CTATGGCGTG GCTGGAAATG TGTTTATTGA CAGTGGCCGT 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCGTACAAA 
TCAGCTCCAA AACATGAATG GATTGGGAAG AATTCTGCTT CCTGGGCCCT TTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACATAACAGC AAGGAAATAC CCATTGAACC AGCCCCCCAC 
CCCCGGCGTG TAGGCATCCT GCTGGACTAT GATAATGGCT CAATCGCTTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA TACCTTCGAT GTGGCGCTTG CGCAGCCTGT GTGCCCCACC 
TTTACTGTGT GGAACAAGTG TTTGACGATT ATAACTGGTC TTCCCATCCC AGACCATTTG 
GACTGTACAG AACAGCTACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457632.1
CDS	765..2768
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNEPVESINAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQERRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHPRRVGILLDYDNGSIAFYDALNSIHLYTFDVALAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_017602143.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus midline 1 (Mid1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017602143.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
CTCCTGCTTC CCTGTGCACA TAGCCTCTGT TTCAACTGTG CCCACCGCAT CCTGGTCTCT 
CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCTTACAA 
AACATCATTG ACAGGTTTCA GAAAGCATCC GTGAGTGGGC CCAATTCTCC CAGTGAGACC 
CGCAGGGAGC GGGCCTTTGA CGCCAACACC ATGTCTTCTG CCGAGAAAGT TCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACCT GTGTTACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATCCCGGA CTCGCATATC CGGGGGCTAA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAATTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAGGTCA ATGCATCCCG TCAAGAAGCC 
AAGTTGACAG AGGAATGTGA TCTTCTCATT GAAATTATTC AGGAAAGAAG ACAAATTATT 
GGAACAAAGA TTAAAGAAGG CAAGGTGATG AGGCTCCGCA AATTAGCTCA GCAGATTGCA 
AACTGTAAAC AATGCATTGA AAGGTCTGCA TCACTCATTT CCCAAGCAGA ACACTCACTG 
AAGGAGAATG ACCATGCCCG TTTTCTACAG ACAGCAAAGA ACATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTTTGGA TTACCTAACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACT 
GTCCACTGGA CTTCGGATGA TGAGTTCAGT GTGGTCTCCT ATGAACTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT CGTTAGTCTT TGTAACTCGG CAGATAGCTG GATGATTGTG 
CCCAACATCA AGCAGAACCA CTATACTGTG CATGGCCTAC AAAGTGGCAC CAAGTATATC 
TTCATGGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGCTGAAG 
ACAAACAGTC AGCCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT CAAGGTGTCC 
CATGACAACC TGACTGTTGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACTA GCCAAGGGAG CTATGGCGTG GCTGGAAATG TGTTTATTGA CAGTGGCCGT 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCGTACAAA 
TCAGCTCCAA AACATGAATG GATTGGGAAG AATTCTGCTT CCTGGGCCCT TTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACATAACAGC AAGGAAATAC CCATTGAACC AGCCCCCCAC 
CCCCGGCGTG TAGGCATCCT GCTGGACTAT GATAATGGCT CAATCGCTTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA TACCTTCGAT GTGGCGCTTG CGCAGCCTGT GTGCCCCACC 
TTTACTGTGT GGAACAAGTG TTTGACGATT ATAACTGGTC TTCCCATCCC AGACCATTTG 
GACTGTACAG AACAGCTACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid1 ( XM_006256825.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256887.1 Rattus norvegicus midline 1 (Mid1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39661
Clone ID Related Accession (Same CDS sequence)	XM_017602143.1 , XM_006256825.3 , XM_017602142.1
Accession Version	XM_006256825.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
CTCCTGCTTC CCTGTGCACA TAGCCTCTGT TTCAACTGTG CCCACCGCAT CCTGGTCTCT 
CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCTTACAA 
AACATCATTG ACAGGTTTCA GAAAGCATCC GTGAGTGGGC CCAATTCTCC CAGTGAGACC 
CGCAGGGAGC GGGCCTTTGA CGCCAACACC ATGTCTTCTG CCGAGAAAGT TCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACCT GTGTTACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATCCCGGA CTCGCATATC CGGGGGCTAA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAATTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAGGTCA ATGCATCCCG TCAAGAAGCC 
AAGTTGACAG AGGAATGTGA TCTTCTCATT GAAATTATTC AGGAAAGAAG ACAAATTATT 
GGAACAAAGA TTAAAGAAGG CAAGGTGATG AGGCTCCGCA AATTAGCTCA GCAGATTGCA 
AACTGTAAAC AATGCATTGA AAGGTCTGCA TCACTCATTT CCCAAGCAGA ACACTCACTG 
AAGGAGAATG ACCATGCCCG TTTTCTACAG ACAGCAAAGA ACATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTTTGGA TTACCTAACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACT 
GTCCACTGGA CTTCGGATGA TGAGTTCAGT GTGGTCTCCT ATGAACTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT CGTTAGTCTT TGTAACTCGG CAGATAGCTG GATGATTGTG 
CCCAACATCA AGCAGAACCA CTATACTGTG CATGGCCTAC AAAGTGGCAC CAAGTATATC 
TTCATGGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGCTGAAG 
ACAAACAGTC AGCCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT CAAGGTGTCC 
CATGACAACC TGACTGTTGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACTA GCCAAGGGAG CTATGGCGTG GCTGGAAATG TGTTTATTGA CAGTGGCCGT 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCGTACAAA 
TCAGCTCCAA AACATGAATG GATTGGGAAG AATTCTGCTT CCTGGGCCCT TTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACATAACAGC AAGGAAATAC CCATTGAACC AGCCCCCCAC 
CCCCGGCGTG TAGGCATCCT GCTGGACTAT GATAATGGCT CAATCGCTTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA TACCTTCGAT GTGGCGCTTG CGCAGCCTGT GTGCCCCACC 
TTTACTGTGT GGAACAAGTG TTTGACGATT ATAACTGGTC TTCCCATCCC AGACCATTTG 
GACTGTACAG AACAGCTACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256887.1
CDS	1510..3513
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNEPVESINAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQERRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHPRRVGILLDYDNGSIAFYDALNSIHLYTFDVALAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_006256825.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus midline 1 (Mid1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256825.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
CTCCTGCTTC CCTGTGCACA TAGCCTCTGT TTCAACTGTG CCCACCGCAT CCTGGTCTCT 
CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCTTACAA 
AACATCATTG ACAGGTTTCA GAAAGCATCC GTGAGTGGGC CCAATTCTCC CAGTGAGACC 
CGCAGGGAGC GGGCCTTTGA CGCCAACACC ATGTCTTCTG CCGAGAAAGT TCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACCT GTGTTACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATCCCGGA CTCGCATATC CGGGGGCTAA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAATTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAGGTCA ATGCATCCCG TCAAGAAGCC 
AAGTTGACAG AGGAATGTGA TCTTCTCATT GAAATTATTC AGGAAAGAAG ACAAATTATT 
GGAACAAAGA TTAAAGAAGG CAAGGTGATG AGGCTCCGCA AATTAGCTCA GCAGATTGCA 
AACTGTAAAC AATGCATTGA AAGGTCTGCA TCACTCATTT CCCAAGCAGA ACACTCACTG 
AAGGAGAATG ACCATGCCCG TTTTCTACAG ACAGCAAAGA ACATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTTTGGA TTACCTAACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACT 
GTCCACTGGA CTTCGGATGA TGAGTTCAGT GTGGTCTCCT ATGAACTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT CGTTAGTCTT TGTAACTCGG CAGATAGCTG GATGATTGTG 
CCCAACATCA AGCAGAACCA CTATACTGTG CATGGCCTAC AAAGTGGCAC CAAGTATATC 
TTCATGGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGCTGAAG 
ACAAACAGTC AGCCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT CAAGGTGTCC 
CATGACAACC TGACTGTTGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACTA GCCAAGGGAG CTATGGCGTG GCTGGAAATG TGTTTATTGA CAGTGGCCGT 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCGTACAAA 
TCAGCTCCAA AACATGAATG GATTGGGAAG AATTCTGCTT CCTGGGCCCT TTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACATAACAGC AAGGAAATAC CCATTGAACC AGCCCCCCAC 
CCCCGGCGTG TAGGCATCCT GCTGGACTAT GATAATGGCT CAATCGCTTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA TACCTTCGAT GTGGCGCTTG CGCAGCCTGT GTGCCCCACC 
TTTACTGTGT GGAACAAGTG TTTGACGATT ATAACTGGTC TTCCCATCCC AGACCATTTG 
GACTGTACAG AACAGCTACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid1 ( XM_017602142.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457631.1 Rattus norvegicus midline 1 (Mid1), transcript variant X2, mRNA.

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CloneID	ORa39661
Clone ID Related Accession (Same CDS sequence)	XM_017602143.1 , XM_006256825.3 , XM_017602142.1
Accession Version	XM_017602142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
CTCCTGCTTC CCTGTGCACA TAGCCTCTGT TTCAACTGTG CCCACCGCAT CCTGGTCTCT 
CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCTTACAA 
AACATCATTG ACAGGTTTCA GAAAGCATCC GTGAGTGGGC CCAATTCTCC CAGTGAGACC 
CGCAGGGAGC GGGCCTTTGA CGCCAACACC ATGTCTTCTG CCGAGAAAGT TCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACCT GTGTTACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATCCCGGA CTCGCATATC CGGGGGCTAA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAATTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAGGTCA ATGCATCCCG TCAAGAAGCC 
AAGTTGACAG AGGAATGTGA TCTTCTCATT GAAATTATTC AGGAAAGAAG ACAAATTATT 
GGAACAAAGA TTAAAGAAGG CAAGGTGATG AGGCTCCGCA AATTAGCTCA GCAGATTGCA 
AACTGTAAAC AATGCATTGA AAGGTCTGCA TCACTCATTT CCCAAGCAGA ACACTCACTG 
AAGGAGAATG ACCATGCCCG TTTTCTACAG ACAGCAAAGA ACATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTTTGGA TTACCTAACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACT 
GTCCACTGGA CTTCGGATGA TGAGTTCAGT GTGGTCTCCT ATGAACTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT CGTTAGTCTT TGTAACTCGG CAGATAGCTG GATGATTGTG 
CCCAACATCA AGCAGAACCA CTATACTGTG CATGGCCTAC AAAGTGGCAC CAAGTATATC 
TTCATGGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGCTGAAG 
ACAAACAGTC AGCCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT CAAGGTGTCC 
CATGACAACC TGACTGTTGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACTA GCCAAGGGAG CTATGGCGTG GCTGGAAATG TGTTTATTGA CAGTGGCCGT 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCGTACAAA 
TCAGCTCCAA AACATGAATG GATTGGGAAG AATTCTGCTT CCTGGGCCCT TTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACATAACAGC AAGGAAATAC CCATTGAACC AGCCCCCCAC 
CCCCGGCGTG TAGGCATCCT GCTGGACTAT GATAATGGCT CAATCGCTTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA TACCTTCGAT GTGGCGCTTG CGCAGCCTGT GTGCCCCACC 
TTTACTGTGT GGAACAAGTG TTTGACGATT ATAACTGGTC TTCCCATCCC AGACCATTTG 
GACTGTACAG AACAGCTACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457631.1
CDS	378..2381
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNEPVESINAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQERRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHPRRVGILLDYDNGSIAFYDALNSIHLYTFDVALAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_017602142.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus midline 1 (Mid1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017602142.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
CTCCTGCTTC CCTGTGCACA TAGCCTCTGT TTCAACTGTG CCCACCGCAT CCTGGTCTCT 
CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCTTACAA 
AACATCATTG ACAGGTTTCA GAAAGCATCC GTGAGTGGGC CCAATTCTCC CAGTGAGACC 
CGCAGGGAGC GGGCCTTTGA CGCCAACACC ATGTCTTCTG CCGAGAAAGT TCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACCT GTGTTACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATCCCGGA CTCGCATATC CGGGGGCTAA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAATTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAGGTCA ATGCATCCCG TCAAGAAGCC 
AAGTTGACAG AGGAATGTGA TCTTCTCATT GAAATTATTC AGGAAAGAAG ACAAATTATT 
GGAACAAAGA TTAAAGAAGG CAAGGTGATG AGGCTCCGCA AATTAGCTCA GCAGATTGCA 
AACTGTAAAC AATGCATTGA AAGGTCTGCA TCACTCATTT CCCAAGCAGA ACACTCACTG 
AAGGAGAATG ACCATGCCCG TTTTCTACAG ACAGCAAAGA ACATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTTTGGA TTACCTAACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACT 
GTCCACTGGA CTTCGGATGA TGAGTTCAGT GTGGTCTCCT ATGAACTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT CGTTAGTCTT TGTAACTCGG CAGATAGCTG GATGATTGTG 
CCCAACATCA AGCAGAACCA CTATACTGTG CATGGCCTAC AAAGTGGCAC CAAGTATATC 
TTCATGGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGCTGAAG 
ACAAACAGTC AGCCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT CAAGGTGTCC 
CATGACAACC TGACTGTTGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACTA GCCAAGGGAG CTATGGCGTG GCTGGAAATG TGTTTATTGA CAGTGGCCGT 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCGTACAAA 
TCAGCTCCAA AACATGAATG GATTGGGAAG AATTCTGCTT CCTGGGCCCT TTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACATAACAGC AAGGAAATAC CCATTGAACC AGCCCCCCAC 
CCCCGGCGTG TAGGCATCCT GCTGGACTAT GATAATGGCT CAATCGCTTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA TACCTTCGAT GTGGCGCTTG CGCAGCCTGT GTGCCCCACC 
TTTACTGTGT GGAACAAGTG TTTGACGATT ATAACTGGTC TTCCCATCCC AGACCATTTG 
GACTGTACAG AACAGCTACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic analysis of dimeric E3-RING interactions reveals increased combinatorial complexity in human ubiquitination networks.
Molecular & cellular proteomics : MCP11(7)M111.016162(2012 Jul)
Woodsmith J,Jenn RC,Sanderson CM

TRIM27 negatively regulates NOD2 by ubiquitination and proteasomal degradation.
PloS one7(7)e41255(2012)
Zurek B,Schoultz I,Neerincx A,Napolitano LM,Birkner K,Bennek E,Sellge G,Lerm M,Meroni G,S?derholm JD,Kufer TA

Active transport of the ubiquitin ligase MID1 along the microtubules is regulated by protein phosphatase 2A.
PloS one3(10)e3507(2008)
Aranda-Orgill?s B,Aigner J,Kunath M,Lurz R,Schneider R,Schweiger S

Alternative polyadenylation signals and promoters act in concert to control tissue-specific expression of the Opitz Syndrome gene MID1.
BMC molecular biology8105(2007 Nov)
Winter J,Kunath M,Roepcke S,Krause S,Schneider R,Schweiger S

Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3.
Molecular and cellular biology27(12)4217-27(2007 Jun)
Prickett TD,Brautigan DL