Cd274 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd274
Entrez Gene ID	499342
Full Name	CD274 molecule
Synonyms	Pdcd1lg1,RGD1566211
General protein information	Preferred Names CD274 molecule Names programmed cell death 1 ligand 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q52

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191954.1	NP_001178883.1	programmed cell death 1 ligand 1 precursor
XM_006231248.2	XP_006231310.1	programmed cell death 1 ligand 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CD274	NP_054862.1
Canis lupus familiaris (dog)	CD274	XP_005615991.1
Bos taurus (cattle)	CD274	NP_001156884.1
Mus musculus (house mouse)	Cd274	NP_068693.1
Gallus gallus (chicken)	CD274	XP_424811.4
Pan troglodytes (chimpanzee)	CD274	XP_001140705.1
Rattus norvegicus (Norway rat)	Cd274	NP_001178883.1
Danio rerio (zebrafish)	si:ch211-241b2.5	XP_003200855.2
	CD274	NP_001077358.1
Xenopus tropicalis (tropical clawed frog)	cd274	XP_004911464.1

Gene Ontology
Bibliography

Related articles in PubMed

CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity.
Burr ML, Sparbier CE, Chan YC, Williamson JC, Woods K, Beavis PA, Lam EYN, Henderson MA, Bell CC, Stolzenburg S, Gilan O, Bloor S, Noori T, Morgens DW, Bassik MC, Neeson PJ, Behren A, Darcy PK, Dawson SJ, Voskoboinik I, Trapani JA, Cebon J, Lehner PJ, Dawson MA
Nature549(7670)101-105(2017 09)

Identification of CMTM6 and CMTM4 as PD-L1 protein regulators.
Mezzadra R, Sun C, Jae LT, Gomez-Eerland R, de Vries E, Wu W, Logtenberg MEW, Slagter M, Rozeman EA, Hofland I, Broeks A, Horlings HM, Wessels LFA, Blank CU, Xiao Y, Heck AJR, Borst J, Brummelkamp TR, Schumacher TNM
Nature549(7670)106-110(2017 09)

Role of programmed death ligand 1 and Kupffer cell in immune regulation after orthotopic liver transplantation in rats.
Gong J, Cao D, Chen Y, Li J, Gong J, Zeng Z
International immunopharmacology488-16(2017 Jul)

Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.
Wu J, Sun L, Li H, Shen H, Zhai W, Yu Z, Chen G
Journal of neuroinflammation14(1)36(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Programmed death-ligand 1 expression increases after intracerebral hemorrhage.
Title: Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.

Upregulation of PD-1 and PD-L1 was associated with marked increases in GADD153 during myocardial ischemic reperfusion injury.
Title: Upregulation of Programmed Death-1 and Its Ligand in Cardiac Injury Models: Interaction with GADD153.

Findings suggested that DC/Ad-PD-L1 could induce CD8+ T cell tolerance and lead to kidney allograft tolerance, which provided a promising finding for clinical application.
Title: Dendritic cells transfected with PD-L1 recombinant adenovirus induces T cell suppression and long-term acceptance of allograft transplantation.

The following Cd274 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd274 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08116	NM_001191954.1 Latest version!	Rattus norvegicus CD274 molecule (Cd274), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa08116	XM_006231248.2 Latest version!	Rattus norvegicus CD274 molecule (Cd274), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd274 ( NM_001191954.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178883.1 Rattus norvegicus CD274 molecule (Cd274), mRNA.

Price & Availability↑

CloneID	ORa08116
Clone ID Related Accession (Same CDS sequence)	NM_001191954.1 , XM_006231248.2
Accession Version	NM_001191954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	programmed cell death 1 ligand 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC109391.5. On Jul 17, 2010 this sequence version replaced XM_574652.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGGATAT TTGCTGTCCT TATAGTCACA GCCTGCAGTC ACGTGCTAGC GGCATTTACC 
ATCACAGCTC CAAAGGACCT GTACGTGGTG GAGTATGGCA GCAATGTCAC GATGGAATGC 
AGATTCCCAG TAGAACAGAA ATTGGACCTG CTTGCCTTAG TGGTGTACTG GGAAAAGGAA 
GACAAGGAAG TTATTCAGTT TGTGGAGGGA GAGGAGGACC TGAAGCCTCA ACACAGCAGC 
TTCAGGGGGA GAGCCTTCTT GCCAAAGGAC CAGCTTTTGA AGGGGAACGC GGTGCTTCAG 
ATCACAGATG TCAAGCTGCA GGACGCAGGT GTCTACTGCT GCATGATCAG CTATGGTGGA 
GCGGACTACA AGCGAATCAC ATTGAAAGTC AACGCTCCAT ACCGCAAAAT CAACCAAAGA 
ATTTCCATGG ATCCAGCCAC TTCTGAGCAT GAACTAATGT GCCAGGCTGA GGGTTACCCA 
GAAGCCGAAG TGATCTGGAC AAACAGTGAC CACCAGTCCC TGAGTGGGGA AACAACTGTC 
ACCACTTCCC AGACTGAGGA GAAGCTTCTC AACGTGACCA GCGTTCTGAG GGTCAACGCA 
ACAGCTAATG ATGTTTTCCA CTGTACGTTC TGGAGAGTAC ACTCAGGGGA GAACCACACG 
GCTGAACTGA TCATCCCAGA ACTGCCTGTA CCACGTCTCC CACATAACAG GACACACTGG 
GTACTCCTGG GATCCGTCCT TTTGTTCCTC ATCGTGGGGT TCACCGTCTT CTTCTGCTTG 
AGAAAACAAG TGAGAATGCT AGATGTGGAA AAATGCGGCT TCGAAGATAG AAATTCAAAG 
AACCGAAATG ATACACAGTT CGAGGAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178883.1
CDS	1..873
Translation	MRIFAVLIVTACSHVLAAFTITAPKDLYVVEYGSNVTMECRFPVEQKLDLLALVVYWEKEDKEVIQFVEGEEDLKPQHSSFRGRAFLPKDQLLKGNAVLQITDVKLQDAGVYCCMISYGGADYKRITLKVNAPYRKINQRISMDPATSEHELMCQAEGYPEAEVIWTNSDHQSLSGETTVTTSQTEEKLLNVTSVLRVNATANDVFHCTFWRVHSGENHTAELIIPELPVPRLPHNRTHWVLLGSVLLFLIVGFTVFFCLRKQVRMLDVEKCGFEDRNSKNRNDTQFEET

Target ORF information:

RefSeq Version	NM_001191954.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CD274 molecule (Cd274), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191954.1

ORF Insert Sequence:

ATGAGGATAT TTGCTGTCCT TATAGTCACA GCCTGCAGTC ACGTGCTAGC GGCATTTACC 
ATCACAGCTC CAAAGGACCT GTACGTGGTG GAGTATGGCA GCAATGTCAC GATGGAATGC 
AGATTCCCAG TAGAACAGAA ATTGGACCTG CTTGCCTTAG TGGTGTACTG GGAAAAGGAA 
GACAAGGAAG TTATTCAGTT TGTGGAGGGA GAGGAGGACC TGAAGCCTCA ACACAGCAGC 
TTCAGGGGGA GAGCCTTCTT GCCAAAGGAC CAGCTTTTGA AGGGGAACGC GGTGCTTCAG 
ATCACAGATG TCAAGCTGCA GGACGCAGGT GTCTACTGCT GCATGATCAG CTATGGTGGA 
GCGGACTACA AGCGAATCAC ATTGAAAGTC AACGCTCCAT ACCGCAAAAT CAACCAAAGA 
ATTTCCATGG ATCCAGCCAC TTCTGAGCAT GAACTAATGT GCCAGGCTGA GGGTTACCCA 
GAAGCCGAAG TGATCTGGAC AAACAGTGAC CACCAGTCCC TGAGTGGGGA AACAACTGTC 
ACCACTTCCC AGACTGAGGA GAAGCTTCTC AACGTGACCA GCGTTCTGAG GGTCAACGCA 
ACAGCTAATG ATGTTTTCCA CTGTACGTTC TGGAGAGTAC ACTCAGGGGA GAACCACACG 
GCTGAACTGA TCATCCCAGA ACTGCCTGTA CCACGTCTCC CACATAACAG GACACACTGG 
GTACTCCTGG GATCCGTCCT TTTGTTCCTC ATCGTGGGGT TCACCGTCTT CTTCTGCTTG 
AGAAAACAAG TGAGAATGCT AGATGTGGAA AAATGCGGCT TCGAAGATAG AAATTCAAAG 
AACCGAAATG ATACACAGTT CGAGGAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd274 ( XM_006231248.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231310.1 Rattus norvegicus CD274 molecule (Cd274), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa08116
Clone ID Related Accession (Same CDS sequence)	NM_001191954.1 , XM_006231248.2
Accession Version	XM_006231248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	programmed cell death 1 ligand 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT TTGCTGTCCT TATAGTCACA GCCTGCAGTC ACGTGCTAGC GGCATTTACC 
ATCACAGCTC CAAAGGACCT GTACGTGGTG GAGTATGGCA GCAATGTCAC GATGGAATGC 
AGATTCCCAG TAGAACAGAA ATTGGACCTG CTTGCCTTAG TGGTGTACTG GGAAAAGGAA 
GACAAGGAAG TTATTCAGTT TGTGGAGGGA GAGGAGGACC TGAAGCCTCA ACACAGCAGC 
TTCAGGGGGA GAGCCTTCTT GCCAAAGGAC CAGCTTTTGA AGGGGAACGC GGTGCTTCAG 
ATCACAGATG TCAAGCTGCA GGACGCAGGT GTCTACTGCT GCATGATCAG CTATGGTGGA 
GCGGACTACA AGCGAATCAC ATTGAAAGTC AACGCTCCAT ACCGCAAAAT CAACCAAAGA 
ATTTCCATGG ATCCAGCCAC TTCTGAGCAT GAACTAATGT GCCAGGCTGA GGGTTACCCA 
GAAGCCGAAG TGATCTGGAC AAACAGTGAC CACCAGTCCC TGAGTGGGGA AACAACTGTC 
ACCACTTCCC AGACTGAGGA GAAGCTTCTC AACGTGACCA GCGTTCTGAG GGTCAACGCA 
ACAGCTAATG ATGTTTTCCA CTGTACGTTC TGGAGAGTAC ACTCAGGGGA GAACCACACG 
GCTGAACTGA TCATCCCAGA ACTGCCTGTA CCACGTCTCC CACATAACAG GACACACTGG 
GTACTCCTGG GATCCGTCCT TTTGTTCCTC ATCGTGGGGT TCACCGTCTT CTTCTGCTTG 
AGAAAACAAG TGAGAATGCT AGATGTGGAA AAATGCGGCT TCGAAGATAG AAATTCAAAG 
AACCGAAATG ATACACAGTT CGAGGAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231310.1
CDS	103..975
Translation	MRIFAVLIVTACSHVLAAFTITAPKDLYVVEYGSNVTMECRFPVEQKLDLLALVVYWEKEDKEVIQFVEGEEDLKPQHSSFRGRAFLPKDQLLKGNAVLQITDVKLQDAGVYCCMISYGGADYKRITLKVNAPYRKINQRISMDPATSEHELMCQAEGYPEAEVIWTNSDHQSLSGETTVTTSQTEEKLLNVTSVLRVNATANDVFHCTFWRVHSGENHTAELIIPELPVPRLPHNRTHWVLLGSVLLFLIVGFTVFFCLRKQVRMLDVEKCGFEDRNSKNRNDTQFEET

Target ORF information:

RefSeq Version	XM_006231248.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CD274 molecule (Cd274), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231248.2

ORF Insert Sequence:

ATGAGGATAT TTGCTGTCCT TATAGTCACA GCCTGCAGTC ACGTGCTAGC GGCATTTACC 
ATCACAGCTC CAAAGGACCT GTACGTGGTG GAGTATGGCA GCAATGTCAC GATGGAATGC 
AGATTCCCAG TAGAACAGAA ATTGGACCTG CTTGCCTTAG TGGTGTACTG GGAAAAGGAA 
GACAAGGAAG TTATTCAGTT TGTGGAGGGA GAGGAGGACC TGAAGCCTCA ACACAGCAGC 
TTCAGGGGGA GAGCCTTCTT GCCAAAGGAC CAGCTTTTGA AGGGGAACGC GGTGCTTCAG 
ATCACAGATG TCAAGCTGCA GGACGCAGGT GTCTACTGCT GCATGATCAG CTATGGTGGA 
GCGGACTACA AGCGAATCAC ATTGAAAGTC AACGCTCCAT ACCGCAAAAT CAACCAAAGA 
ATTTCCATGG ATCCAGCCAC TTCTGAGCAT GAACTAATGT GCCAGGCTGA GGGTTACCCA 
GAAGCCGAAG TGATCTGGAC AAACAGTGAC CACCAGTCCC TGAGTGGGGA AACAACTGTC 
ACCACTTCCC AGACTGAGGA GAAGCTTCTC AACGTGACCA GCGTTCTGAG GGTCAACGCA 
ACAGCTAATG ATGTTTTCCA CTGTACGTTC TGGAGAGTAC ACTCAGGGGA GAACCACACG 
GCTGAACTGA TCATCCCAGA ACTGCCTGTA CCACGTCTCC CACATAACAG GACACACTGG 
GTACTCCTGG GATCCGTCCT TTTGTTCCTC ATCGTGGGGT TCACCGTCTT CTTCTGCTTG 
AGAAAACAAG TGAGAATGCT AGATGTGGAA AAATGCGGCT TCGAAGATAG AAATTCAAAG 
AACCGAAATG ATACACAGTT CGAGGAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity.
Nature549(7670)101-105(2017 09)
Burr ML,Sparbier CE,Chan YC,Williamson JC,Woods K,Beavis PA,Lam EYN,Henderson MA,Bell CC,Stolzenburg S,Gilan O,Bloor S,Noori T,Morgens DW,Bassik MC,Neeson PJ,Behren A,Darcy PK,Dawson SJ,Voskoboinik I,Trapani JA,Cebon J,Lehner PJ,Dawson MA

Identification of CMTM6 and CMTM4 as PD-L1 protein regulators.
Nature549(7670)106-110(2017 09)
Mezzadra R,Sun C,Jae LT,Gomez-Eerland R,de Vries E,Wu W,Logtenberg MEW,Slagter M,Rozeman EA,Hofland I,Broeks A,Horlings HM,Wessels LFA,Blank CU,Xiao Y,Heck AJR,Borst J,Brummelkamp TR,Schumacher TNM

Role of programmed death ligand 1 and Kupffer cell in immune regulation after orthotopic liver transplantation in rats.
International immunopharmacology488-16(2017 Jul)
Gong J,Cao D,Chen Y,Li J,Gong J,Zeng Z

Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.
Journal of neuroinflammation14(1)36(2017 Feb)
Wu J,Sun L,Li H,Shen H,Zhai W,Yu Z,Chen G