Smcr8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smcr8
Entrez Gene ID	497918
Full Name	Smith-Magenis syndrome chromosome region, candidate 8
Synonyms	RGD1564621
General protein information	Preferred Names Smith-Magenis syndrome chromosome region, candidate 8 Names Smith-Magenis syndrome chromosomal region candidate gene 8 protein Smith-Magenis syndrome chromosome region, candidate 8 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191919.1	NP_001178848.1	Smith-Magenis syndrome chromosomal region candidate gene 8 protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMCR8	XP_851653.1
Rattus norvegicus (Norway rat)	Smcr8	NP_001178848.1
Homo sapiens (human)	SMCR8	NP_658988.2
Bos taurus (cattle)	SMCR8	XP_003587459.2
Danio rerio (zebrafish)	smcr8a	XP_687034.3
	SMCR8	XP_001095753.1
Xenopus tropicalis (tropical clawed frog)	smcr8	XP_002935890.1
Gallus gallus (chicken)	SMCR8	XP_004945263.1
Pan troglodytes (chimpanzee)	SMCR8	XP_001157693.1
Mus musculus (house mouse)	Smcr8	NP_001078909.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.
Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch AK, M?ller S, Dikic I, Mittelbronn M, Behrends C
eLife6(2017 02)

C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling.
Amick J, Roczniak-Ferguson A, Ferguson SM
Molecular biology of the cell27(20)3040-3051(2016 Oct)

A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy.
Yang M, Liang C, Swaminathan K, Herrlinger S, Lai F, Shiekhattar R, Chen JF
Science advances2(9)e1601167(2016 09)

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
Sellier C, Campanari ML, Julie Corbier C, Gaucherot A, Kolb-Cheynel I, Oulad-Abdelghani M, Ruffenach F, Page A, Ciura S, Kabashi E, Charlet-Berguerand N
The EMBO journal35(12)1276-97(2016 06)

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.
Sullivan PM, Zhou X, Robins AM, Paushter DH, Kim D, Smolka MB, Hu F
Acta neuropathologica communications4(1)51(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Smcr8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smcr8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08028	NM_001191919.1 Latest version!	Rattus norvegicus SMCR8-C9orf72 complex subunit (Smcr8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smcr8 ( NM_001191919.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178848.1 Rattus norvegicus SMCR8-C9orf72 complex subunit (Smcr8), mRNA.

Price & Availability↑

CloneID	ORa08028
Clone ID Related Accession (Same CDS sequence)	NM_001191919.1
Accession Version	NM_001191919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Rattus norvegicus(Norway rat)
Product	Smith-Magenis syndrome chromosomal region candidate gene 8 protein
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AABR07072125.1. On Jul 17, 2010 this sequence version replaced XM_573105.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATCAGCG CCCCTGATGT GGTGGCCTTT ACCAAGGAAG ACGAATACGA GGAAGAACCT 
TACAATGAAC CCGCTTTGCC TGAAGAATAC TCAGTTCCTC TCTTTCCTTA TGCCAGCCAG 
GGCGCTAACC CCTGGTCTAA ACTGTCTGGG GCCAAGTTCT CCAGGGACTT TATCCTTATT 
TCAGAGTTCT CTGAGCAGGT GGGACCACAG CCCTTGCTAA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT CAATTACTTC TCCTTGCGGA TCATGTCAGT GGATTATCAG 
GCCTCTTTCG TAGGGCATCC TCCTGGTTCT GCCTACCCCA AGTTGAACTT TGTGGAGGAC 
TCTAAAGTGG TACTTGGAGA CTCTAAGGAA GGGGCATTTG CATATGTGCA CCACCTTACC 
TTGTATGACC TGGAGGCCAG GGGCTTCGTG CGGCCCTTTT GTATGGCTTA TATCTCTGCA 
GACCAGCATA AAATCATGCA GCAATTCCAA GAGCTCTCAG CCGAATTTTC CAAAGCATCT 
GAGTGCTTGA AGACGGGTAA CAGGAAGGCA TTCGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACGAGGAC AGTGCTACAC ACAGAAACCG AGATCCAGAA GAAAGCCAAT 
GACAAGGGTT TTTATTCTTC TCAGGCAATT GAGAAAGCCA ATGAACTGGC CAATGTGGAG 
AAGTCCATCA TTGAACATCA AGATTTGCTG AGGCAGATCC GCTCATACCC TCGTCAGAAG 
ATGAAGAAGC CTGATTTGCA TCCAGGTGAT ACAGAGCATA CCCAGGATCA GGCTGACCAG 
GTATGCCCTA CCTCTAATCC TGAGGAGTCA GCTAATGCAG ACCTTTATAC CTGCAGGCCG 
GCTTACACCC CCAAACTCAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AACTCTGTGA CACTGAGTAT TTCACTGAGA CCCTGGCCCA GCTTAGCCAC 
ATTGAACACA TGTTCAGAGG GGACCTGTGC TACCTCCTGA CCAGTCAGAT TGACAGAGCA 
CTTCGAAAAC AACAGCCCAT AACAAATTTC CTCTTTGAAG ATTTTGTAGA GGTTGATGAC 
AGGATGGAGA AGCAAGAGAA TGTGCCCTCT CAACCCAGTC ACGGCAGGCT GCCTTCCAAG 
CCTGTAGAAG AATGCCCCAT TCCTAAAGTG TTAATTAGTG TTGGCTCTTA CAAGTCCAGT 
GTGGAGTCTG TATTGATCAA GATGGAGCAG GAACTTGGAG ACGAAGAATA CAAGGAAGTA 
GAGGCAACAG AGTCAAGCAG TTTTGACCCC CAAGAAAACC TGGACTACAT GGGTATGGAT 
ATGAAAGGGA GCATCAGTAG TGGGGAAAGC ATTGAGGTTC TGGGCACTGA GAAGTCAGCT 
TCTGTGTTGT CGAAATCTGA CAGCCAGGCT AGCCTCACCG TGCCATTGAG CCCCCATGTA 
GTCCGTAGCA AAGCGGTCAG CCACAGGACA ATCAGTGAGG ACAGCATTGA AGTCTTAAGC 
ACCTGCCCTT CTGAGGCCCT CATTCCTGAT GACTTTAAGG CCAGCTACCC AAGTGCCATT 
AATGAAGAAG AAGCCTATGC AGACAATGAG GGGGCCATCC ATTTCCAGGC AAGTGCCAGC 
TCACCAGAAC TAGATGAGAC TCAGGAGGGC AACTTAGAAA ATATCCCATC CCAAATAGAC 
TCCAGCTGCT GTATTGGAAA GGAGAGTGAA GGTCAGTTGG TGCCTCTCCC CACCCCAGCT 
TACACCCTTT CTGATGAGGA TGGTGTGGTG AGCATTCCCC CACAGCGCTA CATACAGAAG 
GACCAGGGGC TCCACATGGA CTTCGGGATG GAGAACACTG ACCCTTCTCC CCGAGACAAC 
AGTTGTGAAA TGTTCCCAGC TTATGATCTG GATCCAAGCT GCCTTCTGGC TAGCCGAGAT 
ATTAGTAAGA TGAGCCTGGA TAACTACTCG GATAGCACTA GCTACATGGG CAGTGTGGCC 
TCTACCAGCT CGGACAGAAT CCCCTCTGCT CCTCCTGCTG GCCTATCCTC TGAGAGGCAC 
AAAAAGAGAG CTGGCCAGAA TGCCTTAAAA TTCATCCGCC AGTACCCTTT TGCCCACCCA 
GCCATCTACT CCCTGCTCAG TGGGAGGACA CTTGTGGTCC TGGGCGAAGA TGAAACCATA 
GTCAGGAAGC TGGTAACTGC ACTGTCCATC TTCGTTCCCA ACTATGGCTG CTATGCCAAG 
CCCGTGAAGC ACTGGATTTC CTCCCCTTTG CATATTATGG ATTTTCAGAA GTGGAAGCTT 
ATTGGCCTAC AAAGAGTGGC CTCTCCTGCC AATGTGGGTA CCCTTCATAC CCTGAGCCGT 
TACAGCCGCT ATACAAGCAT CCTGGACCTG GACAGCAAGA CTCTACGTTG TCCCCTGTAC 
AGGGGTACAC TGGTACCCCG CCTGGCTGAC CACCGCACTC AGATCAAGCG GGGCAGCACA 
TACTACCTTC ATGTCCAGAG CATGCTCACC CAGCTCTGCT CCAAGGCATT CCTCTATACC 
TTTTGCCACC ACTTGCATTT GCCTGCTCAC AGTGAGGAGA CACAAGAAGC AGTGGCCAGC 
AGACAAACCA GCTTCCTAAA GCTAAACCTG GGACTAGTGA ATGAGGACAT CAGAGTGGTA 
CAGTACCTGG CCGAGCTGTT GAAATTGCAC TATATGCAGG AGTCACCTGG GACCACCCAC 
CCACTGCTCA GGTTTGACTA TGTCCCCAGC TTTTTATACA AAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178848.1
CDS	123..2930
Translation	MISAPDVVAFTKEDEYEEEPYNEPALPEEYSVPLFPYASQGANPWSKLSGAKFSRDFILISEFSEQVGPQPLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSKASECLKTGNRKAFAGELEKKLKDLDYTRTVLHTETEIQKKANDKGFYSSQAIEKANELANVEKSIIEHQDLLRQIRSYPRQKMKKPDLHPGDTEHTQDQADQVCPTSNPEESANADLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTETLAQLSHIEHMFRGDLCYLLTSQIDRALRKQQPITNFLFEDFVEVDDRMEKQENVPSQPSHGRLPSKPVEECPIPKVLISVGSYKSSVESVLIKMEQELGDEEYKEVEATESSSFDPQENLDYMGMDMKGSISSGESIEVLGTEKSASVLSKSDSQASLTVPLSPHVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEEAYADNEGAIHFQASASSPELDETQEGNLENIPSQIDSSCCIGKESEGQLVPLPTPAYTLSDEDGVVSIPPQRYIQKDQGLHMDFGMENTDPSPRDNSCEMFPAYDLDPSCLLASRDISKMSLDNYSDSTSYMGSVASTSSDRIPSAPPAGLSSERHKKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDETIVRKLVTALSIFVPNYGCYAKPVKHWISSPLHIMDFQKWKLIGLQRVASPANVGTLHTLSRYSRYTSILDLDSKTLRCPLYRGTLVPRLADHRTQIKRGSTYYLHVQSMLTQLCSKAFLYTFCHHLHLPAHSEETQEAVASRQTSFLKLNLGLVNEDIRVVQYLAELLKLHYMQESPGTTHPLLRFDYVPSFLYKI

Target ORF information:

RefSeq Version	NM_001191919.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMCR8-C9orf72 complex subunit (Smcr8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191919.1

ORF Insert Sequence:

ATGATCAGCG CCCCTGATGT GGTGGCCTTT ACCAAGGAAG ACGAATACGA GGAAGAACCT 
TACAATGAAC CCGCTTTGCC TGAAGAATAC TCAGTTCCTC TCTTTCCTTA TGCCAGCCAG 
GGCGCTAACC CCTGGTCTAA ACTGTCTGGG GCCAAGTTCT CCAGGGACTT TATCCTTATT 
TCAGAGTTCT CTGAGCAGGT GGGACCACAG CCCTTGCTAA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT CAATTACTTC TCCTTGCGGA TCATGTCAGT GGATTATCAG 
GCCTCTTTCG TAGGGCATCC TCCTGGTTCT GCCTACCCCA AGTTGAACTT TGTGGAGGAC 
TCTAAAGTGG TACTTGGAGA CTCTAAGGAA GGGGCATTTG CATATGTGCA CCACCTTACC 
TTGTATGACC TGGAGGCCAG GGGCTTCGTG CGGCCCTTTT GTATGGCTTA TATCTCTGCA 
GACCAGCATA AAATCATGCA GCAATTCCAA GAGCTCTCAG CCGAATTTTC CAAAGCATCT 
GAGTGCTTGA AGACGGGTAA CAGGAAGGCA TTCGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACGAGGAC AGTGCTACAC ACAGAAACCG AGATCCAGAA GAAAGCCAAT 
GACAAGGGTT TTTATTCTTC TCAGGCAATT GAGAAAGCCA ATGAACTGGC CAATGTGGAG 
AAGTCCATCA TTGAACATCA AGATTTGCTG AGGCAGATCC GCTCATACCC TCGTCAGAAG 
ATGAAGAAGC CTGATTTGCA TCCAGGTGAT ACAGAGCATA CCCAGGATCA GGCTGACCAG 
GTATGCCCTA CCTCTAATCC TGAGGAGTCA GCTAATGCAG ACCTTTATAC CTGCAGGCCG 
GCTTACACCC CCAAACTCAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AACTCTGTGA CACTGAGTAT TTCACTGAGA CCCTGGCCCA GCTTAGCCAC 
ATTGAACACA TGTTCAGAGG GGACCTGTGC TACCTCCTGA CCAGTCAGAT TGACAGAGCA 
CTTCGAAAAC AACAGCCCAT AACAAATTTC CTCTTTGAAG ATTTTGTAGA GGTTGATGAC 
AGGATGGAGA AGCAAGAGAA TGTGCCCTCT CAACCCAGTC ACGGCAGGCT GCCTTCCAAG 
CCTGTAGAAG AATGCCCCAT TCCTAAAGTG TTAATTAGTG TTGGCTCTTA CAAGTCCAGT 
GTGGAGTCTG TATTGATCAA GATGGAGCAG GAACTTGGAG ACGAAGAATA CAAGGAAGTA 
GAGGCAACAG AGTCAAGCAG TTTTGACCCC CAAGAAAACC TGGACTACAT GGGTATGGAT 
ATGAAAGGGA GCATCAGTAG TGGGGAAAGC ATTGAGGTTC TGGGCACTGA GAAGTCAGCT 
TCTGTGTTGT CGAAATCTGA CAGCCAGGCT AGCCTCACCG TGCCATTGAG CCCCCATGTA 
GTCCGTAGCA AAGCGGTCAG CCACAGGACA ATCAGTGAGG ACAGCATTGA AGTCTTAAGC 
ACCTGCCCTT CTGAGGCCCT CATTCCTGAT GACTTTAAGG CCAGCTACCC AAGTGCCATT 
AATGAAGAAG AAGCCTATGC AGACAATGAG GGGGCCATCC ATTTCCAGGC AAGTGCCAGC 
TCACCAGAAC TAGATGAGAC TCAGGAGGGC AACTTAGAAA ATATCCCATC CCAAATAGAC 
TCCAGCTGCT GTATTGGAAA GGAGAGTGAA GGTCAGTTGG TGCCTCTCCC CACCCCAGCT 
TACACCCTTT CTGATGAGGA TGGTGTGGTG AGCATTCCCC CACAGCGCTA CATACAGAAG 
GACCAGGGGC TCCACATGGA CTTCGGGATG GAGAACACTG ACCCTTCTCC CCGAGACAAC 
AGTTGTGAAA TGTTCCCAGC TTATGATCTG GATCCAAGCT GCCTTCTGGC TAGCCGAGAT 
ATTAGTAAGA TGAGCCTGGA TAACTACTCG GATAGCACTA GCTACATGGG CAGTGTGGCC 
TCTACCAGCT CGGACAGAAT CCCCTCTGCT CCTCCTGCTG GCCTATCCTC TGAGAGGCAC 
AAAAAGAGAG CTGGCCAGAA TGCCTTAAAA TTCATCCGCC AGTACCCTTT TGCCCACCCA 
GCCATCTACT CCCTGCTCAG TGGGAGGACA CTTGTGGTCC TGGGCGAAGA TGAAACCATA 
GTCAGGAAGC TGGTAACTGC ACTGTCCATC TTCGTTCCCA ACTATGGCTG CTATGCCAAG 
CCCGTGAAGC ACTGGATTTC CTCCCCTTTG CATATTATGG ATTTTCAGAA GTGGAAGCTT 
ATTGGCCTAC AAAGAGTGGC CTCTCCTGCC AATGTGGGTA CCCTTCATAC CCTGAGCCGT 
TACAGCCGCT ATACAAGCAT CCTGGACCTG GACAGCAAGA CTCTACGTTG TCCCCTGTAC 
AGGGGTACAC TGGTACCCCG CCTGGCTGAC CACCGCACTC AGATCAAGCG GGGCAGCACA 
TACTACCTTC ATGTCCAGAG CATGCTCACC CAGCTCTGCT CCAAGGCATT CCTCTATACC 
TTTTGCCACC ACTTGCATTT GCCTGCTCAC AGTGAGGAGA CACAAGAAGC AGTGGCCAGC 
AGACAAACCA GCTTCCTAAA GCTAAACCTG GGACTAGTGA ATGAGGACAT CAGAGTGGTA 
CAGTACCTGG CCGAGCTGTT GAAATTGCAC TATATGCAGG AGTCACCTGG GACCACCCAC 
CCACTGCTCA GGTTTGACTA TGTCCCCAGC TTTTTATACA AAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.
eLife6(2017 02)
Jung J,Nayak A,Schaeffer V,Starzetz T,Kirsch AK,M?ller S,Dikic I,Mittelbronn M,Behrends C

C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling.
Molecular biology of the cell27(20)3040-3051(2016 Oct)
Amick J,Roczniak-Ferguson A,Ferguson SM

A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy.
Science advances2(9)e1601167(2016 09)
Yang M,Liang C,Swaminathan K,Herrlinger S,Lai F,Shiekhattar R,Chen JF

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
The EMBO journal35(12)1276-97(2016 06)
Sellier C,Campanari ML,Julie Corbier C,Gaucherot A,Kolb-Cheynel I,Oulad-Abdelghani M,Ruffenach F,Page A,Ciura S,Kabashi E,Charlet-Berguerand N

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.
Acta neuropathologica communications4(1)51(2016 May)
Sullivan PM,Zhou X,Robins AM,Paushter DH,Kim D,Smolka MB,Hu F