Nhlrc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nhlrc1
Entrez Gene ID	364682
Full Name	NHL repeat containing E3 ubiquitin protein ligase 1
Synonyms	Epm2b
General protein information	Preferred Names NHL repeat containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase NHLRC1 NHL repeat containing 1 NHL repeat-containing protein 1 RING-type E3 ubiquitin transferase NHLRC1 malin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	human homolog is a putative E3 ubiquitin ligase associated with Lafora progressive myoclonus epilepsy and possibly involved in clearance of polyglucosans from dendrites [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199236.1	NP_954706.1	E3 ubiquitin-protein ligase NHLRC1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NHLRC1	NP_940988.2
Bos taurus (cattle)	NHLRC1	XP_002697596.1
Gallus gallus (chicken)	NHLRC1	XP_426034.2
Xenopus tropicalis (tropical clawed frog)	nhlrc1	XP_002932735.1
Pan troglodytes (chimpanzee)	NHLRC1	XP_001170828.1
Canis lupus familiaris (dog)	NHLRC1	NP_001006650.1
Mus musculus (house mouse)	Nhlrc1	NP_780549.1
	NHLRC1	XP_001097330.1
Rattus norvegicus (Norway rat)	Nhlrc1	NP_954706.1

Gene Ontology
Bibliography

Related articles in PubMed

Abnormal glycogen chain length pattern, not hyperphosphorylation, is critical in Lafora disease.
Nitschke F, Sullivan MA, Wang P, Zhao X, Chown EE, Perri AM, Israelian L, Juana-L?pez L, Bovolenta P, Rodr?guez de C?rdoba S, Steup M, Minassian BA
EMBO molecular medicine9(7)906-917(2017 Jul)

Homeostasis of the astrocytic glutamate transporter GLT-1 is altered in mouse models of Lafora disease.
Mu?oz-Ballester C, Berthier A, Viana R, Sanz P
Biochimica et biophysica acta1862(6)1074-83(2016 Jun)

Glycogen phosphomonoester distribution in mouse models of the progressive myoclonic epilepsy, Lafora disease.
DePaoli-Roach AA, Contreras CJ, Segvich DM, Heiss C, Ishihara M, Azadi P, Roach PJ
The Journal of biological chemistry290(2)841-50(2015 Jan)

Protein degradation and quality control in cells from laforin and malin knockout mice.
Garyali P, Segvich DM, DePaoli-Roach AA, Roach PJ
The Journal of biological chemistry289(30)20606-14(2014 Jul)

Hyperphosphorylation of glucosyl C6 carbons and altered structure of glycogen in the neurodegenerative epilepsy Lafora disease.
Nitschke F, Wang P, Schmieder P, Girard JM, Awrey DE, Wang T, Israelian J, Zhao X, Turnbull J, Heydenreich M, Kleinpeter E, Steup M, Minassian BA
Cell metabolism17(5)756-67(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nhlrc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nhlrc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13751	NM_199236.1 Latest version!	Rattus norvegicus NHL repeat containing E3 ubiquitin protein ligase 1 (Nhlrc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nhlrc1 ( NM_199236.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954706.1 Rattus norvegicus NHL repeat containing E3 ubiquitin protein ligase 1 (Nhlrc1), mRNA.

Price & Availability↑

CloneID	ORa13751
Clone ID Related Accession (Same CDS sequence)	NM_199236.1
Accession Version	NM_199236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase NHLRC1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK001524.1. On Jan 2, 2004 this sequence version replaced XM_344577.1. ##Evidence-Data-START## Transcript exon combination :: BK001524.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGGGAGG AGGCAGCGGG GGTGCGGCCC GAGCTGGTGC GCGAGGCGGA GGTCAGCCTG 
CTGGAGTGCA AGGTGTGCTT CGAGAGGTTC GGCCACCGGC AGCAGCGGCG CCCGCGCAAC 
CTGCCCTGCG GCCACGTGGT CTGCTTGGCC TGCGTCGCCG CCCTCGCGCA CCCGCGGACG 
CTGGCCCTCG AGTGTCCCTT CTGCCGGCGG GCCTGCCGAG CTTGTGACAC CAGCGATTGC 
CTGCCGGTGC TGCACCTTCT GGAGCTCCTG GGCTCCACCC TCCACGCGTC CCCGGCTGCC 
CTCAGCGCCG CCTCCTGTGC GCCCGGGGCT CTCACCTGCT ACCATGCCTT TGGCGGGTGG 
GGGACCCTAG TGAACCCCAC GGGGCTTGCA CTGTGCCCCA AGACCGGACG GGTAGTAGTC 
GTGCACGACG GTAAGAGACG GGTCAAGATC TTCGATTCCG GAGGAGGAGG TGCACACCAG 
TTTGGAGAGA AGGGGGACGC AGCGCATGAC GTGAAGTACC CACTGGATGT CGCGGTCACC 
AACGACTGCC ATGTGGTTGT CACCGACGCC GGCGACTGCT CCCTCAAAGT GTTTGATTTC 
TTTGGCCAGA TCAAGCTGGT TGTGGGAAAG CAGTTTTCCC TGCCTTGGGG TGTAGAGATA 
ACCCCTCACA ACGGGGTCCT GGTGACTGAC GCAGAGGCAG GGACTTTGCA CCTGCTGGAA 
GCGGATTTCC CTGAAGGGGT CCTTCGGAGA ATTGAGAGGT TGCAAGCTCA CCTGTGTAAT 
CCCCGTGGGG TGGCAGTGTC ATGGCTCACC GGGGCCATCG CGGTCCTAGA GCATCCTTGT 
GCCTTGGGGA CGTCAAGCGG TAACAACACA AGGGTGAAGG TGTTCAACTC CAGTATGCAG 
CTGATTGGCC AGGTGGACAG CTTCGGACTG AACCTCCTCT TCCCCTCCAA AATAACCGCC 
TCAGCTGTGA CCTTCGATCA CCAAGGAAAC GTGATTGTTG CTGATACCTC TGGGCCAGCC 
ATCGTATGCT TGGGGAAACC TGAGGAATTT CCAGCCCTGA AGCCTATGGT TACTCATGGC 
CTTTCCCGTC CTGTGGCACT GGTCTTCACC AAGGAGAATT CTCTTCTTGT GCTGGATTCT 
GCATCCCATT CTATAAAAGT CTTTAAAGTG ATGGAGGGTA ATGGAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954706.1
CDS	1..1191
Translation	MGEEAAGVRPELVREAEVSLLECKVCFERFGHRQQRRPRNLPCGHVVCLACVAALAHPRTLALECPFCRRACRACDTSDCLPVLHLLELLGSTLHASPAALSAASCAPGALTCYHAFGGWGTLVNPTGLALCPKTGRVVVVHDGKRRVKIFDSGGGGAHQFGEKGDAAHDVKYPLDVAVTNDCHVVVTDAGDCSLKVFDFFGQIKLVVGKQFSLPWGVEITPHNGVLVTDAEAGTLHLLEADFPEGVLRRIERLQAHLCNPRGVAVSWLTGAIAVLEHPCALGTSSGNNTRVKVFNSSMQLIGQVDSFGLNLLFPSKITASAVTFDHQGNVIVADTSGPAIVCLGKPEEFPALKPMVTHGLSRPVALVFTKENSLLVLDSASHSIKVFKVMEGNGG

Target ORF information:

RefSeq Version	NM_199236.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHL repeat containing E3 ubiquitin protein ligase 1 (Nhlrc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199236.1

ORF Insert Sequence:

ATGGGGGAGG AGGCAGCGGG GGTGCGGCCC GAGCTGGTGC GCGAGGCGGA GGTCAGCCTG 
CTGGAGTGCA AGGTGTGCTT CGAGAGGTTC GGCCACCGGC AGCAGCGGCG CCCGCGCAAC 
CTGCCCTGCG GCCACGTGGT CTGCTTGGCC TGCGTCGCCG CCCTCGCGCA CCCGCGGACG 
CTGGCCCTCG AGTGTCCCTT CTGCCGGCGG GCCTGCCGAG CTTGTGACAC CAGCGATTGC 
CTGCCGGTGC TGCACCTTCT GGAGCTCCTG GGCTCCACCC TCCACGCGTC CCCGGCTGCC 
CTCAGCGCCG CCTCCTGTGC GCCCGGGGCT CTCACCTGCT ACCATGCCTT TGGCGGGTGG 
GGGACCCTAG TGAACCCCAC GGGGCTTGCA CTGTGCCCCA AGACCGGACG GGTAGTAGTC 
GTGCACGACG GTAAGAGACG GGTCAAGATC TTCGATTCCG GAGGAGGAGG TGCACACCAG 
TTTGGAGAGA AGGGGGACGC AGCGCATGAC GTGAAGTACC CACTGGATGT CGCGGTCACC 
AACGACTGCC ATGTGGTTGT CACCGACGCC GGCGACTGCT CCCTCAAAGT GTTTGATTTC 
TTTGGCCAGA TCAAGCTGGT TGTGGGAAAG CAGTTTTCCC TGCCTTGGGG TGTAGAGATA 
ACCCCTCACA ACGGGGTCCT GGTGACTGAC GCAGAGGCAG GGACTTTGCA CCTGCTGGAA 
GCGGATTTCC CTGAAGGGGT CCTTCGGAGA ATTGAGAGGT TGCAAGCTCA CCTGTGTAAT 
CCCCGTGGGG TGGCAGTGTC ATGGCTCACC GGGGCCATCG CGGTCCTAGA GCATCCTTGT 
GCCTTGGGGA CGTCAAGCGG TAACAACACA AGGGTGAAGG TGTTCAACTC CAGTATGCAG 
CTGATTGGCC AGGTGGACAG CTTCGGACTG AACCTCCTCT TCCCCTCCAA AATAACCGCC 
TCAGCTGTGA CCTTCGATCA CCAAGGAAAC GTGATTGTTG CTGATACCTC TGGGCCAGCC 
ATCGTATGCT TGGGGAAACC TGAGGAATTT CCAGCCCTGA AGCCTATGGT TACTCATGGC 
CTTTCCCGTC CTGTGGCACT GGTCTTCACC AAGGAGAATT CTCTTCTTGT GCTGGATTCT 
GCATCCCATT CTATAAAAGT CTTTAAAGTG ATGGAGGGTA ATGGAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Abnormal glycogen chain length pattern, not hyperphosphorylation, is critical in Lafora disease.
EMBO molecular medicine9(7)906-917(2017 Jul)
Nitschke F,Sullivan MA,Wang P,Zhao X,Chown EE,Perri AM,Israelian L,Juana-L?pez L,Bovolenta P,Rodr?guez de C?rdoba S,Steup M,Minassian BA

Homeostasis of the astrocytic glutamate transporter GLT-1 is altered in mouse models of Lafora disease.
Biochimica et biophysica acta1862(6)1074-83(2016 Jun)
Mu?oz-Ballester C,Berthier A,Viana R,Sanz P

Glycogen phosphomonoester distribution in mouse models of the progressive myoclonic epilepsy, Lafora disease.
The Journal of biological chemistry290(2)841-50(2015 Jan)
DePaoli-Roach AA,Contreras CJ,Segvich DM,Heiss C,Ishihara M,Azadi P,Roach PJ

Protein degradation and quality control in cells from laforin and malin knockout mice.
The Journal of biological chemistry289(30)20606-14(2014 Jul)
Garyali P,Segvich DM,DePaoli-Roach AA,Roach PJ

Hyperphosphorylation of glucosyl C6 carbons and altered structure of glycogen in the neurodegenerative epilepsy Lafora disease.
Cell metabolism17(5)756-67(2013 May)
Nitschke F,Wang P,Schmieder P,Girard JM,Awrey DE,Wang T,Israelian J,Zhao X,Turnbull J,Heydenreich M,Kleinpeter E,Steup M,Minassian BA