Pea15 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pea15
Entrez Gene ID	364052
Full Name	proliferation and apoptosis adaptor protein 15
Synonyms	Pea15a
General protein information	Preferred Names proliferation and apoptosis adaptor protein 15 Names astrocytic phosphoprotein PEA-15 15 kDa phosphoprotein enriched in astrocytes phosphoprotein enriched in astrocytes 15 phosphoprotein enriched in astrocytes 15A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013231.1	NP_001013249.1	astrocytic phosphoprotein PEA-15

Pathways from BioSystems

Homologies

Homo sapiens (human)	PEA15	NP_003759.1
Bos taurus (cattle)	PEA15	NP_001068924.1
Mus musculus (house mouse)	Pea15a	NP_035193.1
Rattus norvegicus (Norway rat)	Pea15	NP_001013249.1
Pan troglodytes (chimpanzee)	PEA15	XP_003308618.1
Canis lupus familiaris (dog)	PEA15	XP_005640955.1
Danio rerio (zebrafish)	LOC557745	XP_683349.2
Xenopus tropicalis (tropical clawed frog)	pea15	NP_001120300.1

Gene Ontology
Bibliography

Related articles in PubMed

Hyperglycemia aggravates decreases of PEA-15 and its two phosphorylated forms in cerebral ischemia.
Sung JH, Koh PO
The Journal of veterinary medical science79(3)654-660(2017 Mar)

Biomechanical insult switches PEA-15 activity to uncouple its anti-apoptotic function and promote erk mediated tissue remodeling.
Exler RE, Guo X, Chan D, Livne-Bar I, Vicic N, Flanagan JG, Sivak JM
Experimental cell research340(2)283-94(2016 Jan)

Melatonin ameliorates ischemic-like injury-evoked nitrosative stress: Involvement of HtrA2/PED pathways in endothelial cells.
Han F, Tao RR, Zhang GS, Lu YM, Liu LL, Chen YX, Lou YJ, Fukunaga K, Hong ZH
Journal of pineal research50(3)281-91(2011 Apr)

The time course of unconditioned morphine-induced psychomotor sensitization mirrors the phosphorylation of FADD and MEK/ERK in rat striatum: role of PEA-15 as a FADD-ERK binding partner in striatal plasticity.
Ramos-Miguel A, Esteban S, Garc?a-Sevilla JA
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology20(1)49-64(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a diabetic condition may aggravate brain damage through decreasing expression of PEA-15 and phospho-PEA-15 in ischemic brain injury
Title: Hyperglycemia aggravates decreases of PEA-15 and its two phosphorylated forms in cerebral ischemia.

biomechanical insult dramatically switches PEA-15 phosphorylation and function to uncouple its anti-apoptotic activity
Title: Biomechanical insult switches PEA-15 activity to uncouple its anti-apoptotic function and promote erk mediated tissue remodeling.

The protective effect of melatonin is likely mediated, in part, by inhibition of peroxynitrate-mediated nitrosative stress, which in turn relieves imbalance of mitochondrial HtrA2-PED signaling and endothelial cell death.
Title: Melatonin ameliorates ischemic-like injury-evoked nitrosative stress: Involvement of HtrA2/PED pathways in endothelial cells.

disruption of the PED/PEA-15-PLD1 molecular interaction enhances insulin sensitivity in skeletal muscle cells
Title: Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells.

The following Pea15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pea15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03888	NM_001013231.1 Latest version!	Rattus norvegicus proliferation and apoptosis adaptor protein 15 (Pea15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pea15 ( NM_001013231.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013249.1 Rattus norvegicus proliferation and apoptosis adaptor protein 15 (Pea15), mRNA.

Price & Availability↑

CloneID	ORa03888
Clone ID Related Accession (Same CDS sequence)	NM_001013231.1
Accession Version	NM_001013231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	astrocytic phosphoprotein PEA-15
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085766.1. On Apr 20, 2005 this sequence version replaced XM_344175.2. ##Evidence-Data-START## Transcript exon combination :: BC085766.1, EV763356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGT ACGGGACTCT CCTTCAGGAC CTGACCAACA ACATCACCCT TGAAGATCTG 
GAACAGCTCA AGTCAGCCTG CAAGGAGGAC ATCCCCAGTG AGAAGAGTGA AGAGATCACT 
ACAGGCAGTG CCTGGTTTAG CTTCCTGGAG AGCCACAACA AGCTGGACAA AGACAACCTC 
TCCTACATTG AGCACATCTT TGAGATCTCC CGCCGTCCTG ACCTACTCAC GATGGTGGTT 
GACTACAGAA CCCGTGTGCT GAAGATCTCT GAGGAGGATG AGCTGGACAC CAAGCTAACC 
CGTATCCCCA GCGCCAAGAA GTACAAAGAC ATTATCCGGC AGCCCTCTGA GGAAGAAATC 
ATCAAATTGG CTCCCCCACC AAAGAAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013249.1
CDS	89..481
Translation	MAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFSFLESHNKLDKDNLSYIEHIFEISRRPDLLTMVVDYRTRVLKISEEDELDTKLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA

Target ORF information:

RefSeq Version	NM_001013231.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proliferation and apoptosis adaptor protein 15 (Pea15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013231.1

ORF Insert Sequence:

ATGGCAGAGT ACGGGACTCT CCTTCAGGAC CTGACCAACA ACATCACCCT TGAAGATCTG 
GAACAGCTCA AGTCAGCCTG CAAGGAGGAC ATCCCCAGTG AGAAGAGTGA AGAGATCACT 
ACAGGCAGTG CCTGGTTTAG CTTCCTGGAG AGCCACAACA AGCTGGACAA AGACAACCTC 
TCCTACATTG AGCACATCTT TGAGATCTCC CGCCGTCCTG ACCTACTCAC GATGGTGGTT 
GACTACAGAA CCCGTGTGCT GAAGATCTCT GAGGAGGATG AGCTGGACAC CAAGCTAACC 
CGTATCCCCA GCGCCAAGAA GTACAAAGAC ATTATCCGGC AGCCCTCTGA GGAAGAAATC 
ATCAAATTGG CTCCCCCACC AAAGAAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hyperglycemia aggravates decreases of PEA-15 and its two phosphorylated forms in cerebral ischemia.
The Journal of veterinary medical science79(3)654-660(2017 Mar)
Sung JH,Koh PO

Biomechanical insult switches PEA-15 activity to uncouple its anti-apoptotic function and promote erk mediated tissue remodeling.
Experimental cell research340(2)283-94(2016 Jan)
Exler RE,Guo X,Chan D,Livne-Bar I,Vicic N,Flanagan JG,Sivak JM

Melatonin ameliorates ischemic-like injury-evoked nitrosative stress: Involvement of HtrA2/PED pathways in endothelial cells.
Journal of pineal research50(3)281-91(2011 Apr)
Han F,Tao RR,Zhang GS,Lu YM,Liu LL,Chen YX,Lou YJ,Fukunaga K,Hong ZH

The time course of unconditioned morphine-induced psychomotor sensitization mirrors the phosphorylation of FADD and MEK/ERK in rat striatum: role of PEA-15 as a FADD-ERK binding partner in striatal plasticity.
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology20(1)49-64(2010 Jan)
Ramos-Miguel A,Esteban S,Garc?a-Sevilla JA