Pias4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pias4
Entrez Gene ID	362827
Full Name	protein inhibitor of activated STAT, 4
General protein information	Preferred Names protein inhibitor of activated STAT, 4 Names E3 SUMO-protein ligase PIAS4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100757.1	NP_001094227.1	E3 SUMO-protein ligase PIAS4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PIAS4	NP_001076951.1
Gallus gallus (chicken)	PIAS4	XP_003642892.2
Homo sapiens (human)	PIAS4	NP_056981.2
Mus musculus (house mouse)	Pias4	NP_067476.2
Rattus norvegicus (Norway rat)	Pias4	NP_001094227.1
Danio rerio (zebrafish)	pias4a	NP_998568.2
Canis lupus familiaris (dog)	PIAS4	XP_542167.4
Xenopus tropicalis (tropical clawed frog)	pias4	NP_001011455.1

Gene Ontology
Bibliography

Related articles in PubMed

Push back to respond better: regulatory inhibition of the DNA double-strand break response.
Panier S, Durocher D
Nature reviews. Molecular cell biology14(10)661-72(2013 Oct)

DNA damage-inducible SUMOylation of HERC2 promotes RNF8 binding via a novel SUMO-binding Zinc finger.
Danielsen JR, Povlsen LK, Villumsen BH, Streicher W, Nilsson J, Wikstr?m M, Bekker-Jensen S, Mailand N
The Journal of cell biology197(2)179-87(2012 Apr)

SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function.
Cong L, Pakala SB, Ohshiro K, Li DQ, Kumar R
The Journal of biological chemistry286(51)43793-808(2011 Dec)

SYT-SSX1 (synovial sarcoma translocated) regulates PIASy ligase activity to cause overexpression of NCOA3 protein.
Sun Y, Perera J, Rubin BP, Huang J
The Journal of biological chemistry286(21)18623-32(2011 May)

PIASy interacts with p73alpha and regulates cell cycle in HEK293 cells.
Zhang C, Yuan X, Yue L, Fu J, Luo L, Yin Z
Cellular immunology263(2)235-40(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The increase of NCOA3 is essential for SYT-SSX1-mediated synovial sarcoma formation. SYT-SSX1 does so by increasing the sumoylation of NCOA3 through interaction with a SUMO E3 ligase, PIASy, as well as the sumoylation of NEMO.
Title: SYT-SSX1 (synovial sarcoma translocated) regulates PIASy ligase activity to cause overexpression of NCOA3 protein.

The following Pias4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pias4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08223	NM_001100757.1 Latest version!	Rattus norvegicus protein inhibitor of activated STAT, 4 (Pias4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pias4 ( NM_001100757.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094227.1 Rattus norvegicus protein inhibitor of activated STAT, 4 (Pias4), mRNA.

Price & Availability↑

CloneID	ORa08223
Clone ID Related Accession (Same CDS sequence)	NM_001100757.1
Accession Version	NM_001100757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	E3 SUMO-protein ligase PIAS4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC169045.1. On or before Sep 7, 2008 this sequence version replaced XM_343155.3, XM_001075850.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC169045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCAG AGCTGGTGGA GGCCAAAAAC ATGGTGATGA GCTTCCGAGT GTCAGACCTG 
CAGATGCTGC TGGGCTTTGT AGGTCGTAGC AAGAGCGGGC TGAAGCATGA GCTGGTGACC 
AGGGCCTTGC AGCTGGTGCA GTTTGACTGT AGCCCGGAGC TTTTCAAGAA GATCAAAGAG 
CTGTATGAGA CTCGCTATGC CAAGAAGAGT GCAGAGCCCG GCCCACAGGC GCCACGGCCC 
CTGGATCCCC TGGCACTACA CTCCATGCCC AGGACTCCCC TGTCAGGACC CACCGTGGAC 
TACCCCGTGC TGTACGGGAA GTACTTGAAT GGACTGGGGC GCCTGCCCAC CAAGGCCCTT 
AAGCCGGAGG TACGCCTGGT AAAGCTGCCT TTCTTCAACA TGCTGGACGA GTTGCTGAAG 
CCCACTGAGC TGGTCCCACA GAGTGCTGAG AAGCTGCAAG AGAGCCCGTG CATCTTCGCG 
CTGACCCCTC GGCAGGTGGA GATGATCCGC AACTCCAGGG AGCTGCAGCC TGGAGTGAAA 
GCAGTGCAGG TGGTACTGAG GATCTGTTAC TCTGACACCA GCTGTCCACA GGAGGACCAG 
TATCCACCCA ACATCGCTGT GAAAGTCAAC CACAGCTACT GTTCAGTGCC GGGCTACTAT 
CCCTCCAACA AGCCTGGTGT GGAACCCAAG AGGCCCTGCC GCCCCATCAA CCTTACCCAC 
CTCATGTATC TGTCCTCGGC CACCAACCGC ATTACTGTTA CCTGGGGCAA CTATGGCAAG 
AGCTACTCTG TGGCTTTGTA CCTGGTGCGA CAGCTGACCT CCTCAGACCT GCTACAGAGG 
TTGAAGACGA TTGGGGTGAA GCATCCTGAG CTCTGCAAGG CTCTGGTCAA GGAGAAGCTT 
CGCCTAGACC CTGACAGCGA GATCGCTACT ACCGGAGTGC GAGTGTCCCT CATCTGCCCA 
CTGGTGAAGA TGCGGCTCTC GGTGCCCTGC CGTGCAGAGA CCTGCGCACA CCTGCAGTGC 
TTTGATGCCG TGTTCTACCT ACAAATGAAT GAGAAGAAGC CCACGTGGAT GTGCCCTGTG 
TGTGACAAGC CCGCAGCTTA TGACCAGCTC ATCATAGATG GGCTGCTGTC GAAGATCCTG 
AGTGAGTGTG AGGACGCAGA TGAGATTGAG TTCCTGGCGG AAGGTTCATG GCGCCCAATA 
CGTGCAGAGA AGGAACCAAG CTGCAGCCCA CAGGGCCCCA TCCTGGTGCT AGGCACCTCG 
GACGCCAACG GGCTCGCACC AGCCTCCAGC ACCCCGGGCA TGGGAAGTGG CCTGAGTGGC 
CCAGGCGGCG CCGGCGGCGG GGCGGGGGCG GCCGGTGGCC TGGAGAACGG CAAGACAGGC 
GCAGACGTGG TGGACCTCAC ACTGGACAGC TCGTCGTCCT CAGAAGATGA GGATGAGGAC 
GAGGACGACG ATGAGGACGA TGACGAGGGC CCCCGGCCGA AGCGCCGCTG CCCGTTCCAG 
AAGGGACTGG TGCCCGCTTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094227.1
CDS	56..1579
Translation	MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKSAEPGPQAPRPLDPLALHSMPRTPLSGPTVDYPVLYGKYLNGLGRLPTKALKPEVRLVKLPFFNMLDELLKPTELVPQSAEKLQESPCIFALTPRQVEMIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSDLLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAAYDQLIIDGLLSKILSECEDADEIEFLAEGSWRPIRAEKEPSCSPQGPILVLGTSDANGLAPASSTPGMGSGLSGPGGAGGGAGAAGGLENGKTGADVVDLTLDSSSSSEDEDEDEDDDEDDDEGPRPKRRCPFQKGLVPAC

Target ORF information:

RefSeq Version	NM_001100757.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein inhibitor of activated STAT, 4 (Pias4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100757.1

ORF Insert Sequence:

ATGGCGGCAG AGCTGGTGGA GGCCAAAAAC ATGGTGATGA GCTTCCGAGT GTCAGACCTG 
CAGATGCTGC TGGGCTTTGT AGGTCGTAGC AAGAGCGGGC TGAAGCATGA GCTGGTGACC 
AGGGCCTTGC AGCTGGTGCA GTTTGACTGT AGCCCGGAGC TTTTCAAGAA GATCAAAGAG 
CTGTATGAGA CTCGCTATGC CAAGAAGAGT GCAGAGCCCG GCCCACAGGC GCCACGGCCC 
CTGGATCCCC TGGCACTACA CTCCATGCCC AGGACTCCCC TGTCAGGACC CACCGTGGAC 
TACCCCGTGC TGTACGGGAA GTACTTGAAT GGACTGGGGC GCCTGCCCAC CAAGGCCCTT 
AAGCCGGAGG TACGCCTGGT AAAGCTGCCT TTCTTCAACA TGCTGGACGA GTTGCTGAAG 
CCCACTGAGC TGGTCCCACA GAGTGCTGAG AAGCTGCAAG AGAGCCCGTG CATCTTCGCG 
CTGACCCCTC GGCAGGTGGA GATGATCCGC AACTCCAGGG AGCTGCAGCC TGGAGTGAAA 
GCAGTGCAGG TGGTACTGAG GATCTGTTAC TCTGACACCA GCTGTCCACA GGAGGACCAG 
TATCCACCCA ACATCGCTGT GAAAGTCAAC CACAGCTACT GTTCAGTGCC GGGCTACTAT 
CCCTCCAACA AGCCTGGTGT GGAACCCAAG AGGCCCTGCC GCCCCATCAA CCTTACCCAC 
CTCATGTATC TGTCCTCGGC CACCAACCGC ATTACTGTTA CCTGGGGCAA CTATGGCAAG 
AGCTACTCTG TGGCTTTGTA CCTGGTGCGA CAGCTGACCT CCTCAGACCT GCTACAGAGG 
TTGAAGACGA TTGGGGTGAA GCATCCTGAG CTCTGCAAGG CTCTGGTCAA GGAGAAGCTT 
CGCCTAGACC CTGACAGCGA GATCGCTACT ACCGGAGTGC GAGTGTCCCT CATCTGCCCA 
CTGGTGAAGA TGCGGCTCTC GGTGCCCTGC CGTGCAGAGA CCTGCGCACA CCTGCAGTGC 
TTTGATGCCG TGTTCTACCT ACAAATGAAT GAGAAGAAGC CCACGTGGAT GTGCCCTGTG 
TGTGACAAGC CCGCAGCTTA TGACCAGCTC ATCATAGATG GGCTGCTGTC GAAGATCCTG 
AGTGAGTGTG AGGACGCAGA TGAGATTGAG TTCCTGGCGG AAGGTTCATG GCGCCCAATA 
CGTGCAGAGA AGGAACCAAG CTGCAGCCCA CAGGGCCCCA TCCTGGTGCT AGGCACCTCG 
GACGCCAACG GGCTCGCACC AGCCTCCAGC ACCCCGGGCA TGGGAAGTGG CCTGAGTGGC 
CCAGGCGGCG CCGGCGGCGG GGCGGGGGCG GCCGGTGGCC TGGAGAACGG CAAGACAGGC 
GCAGACGTGG TGGACCTCAC ACTGGACAGC TCGTCGTCCT CAGAAGATGA GGATGAGGAC 
GAGGACGACG ATGAGGACGA TGACGAGGGC CCCCGGCCGA AGCGCCGCTG CCCGTTCCAG 
AAGGGACTGG TGCCCGCTTG CTGA