Spast cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spast
Entrez Gene ID	362700
Full Name	spastin
Synonyms	Spg4
General protein information	Preferred Names spastin Names spastin spastic paraplegia 4 (autosomal dominant spastic paraplegia 4 (autosomal dominant; spastin)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q13

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764486.2	XP_008762708.1	spastin isoform X1
XM_008764487.2	XP_008762709.1	spastin isoform X2
XM_008764488.2	XP_008762710.1	spastin isoform X3
NM_001108702.2	NP_001102172.2	spastin

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPAST	NP_055761.2
Oryza sativa (rice)	Os06g0130000	NP_001056686.1
Xenopus tropicalis (tropical clawed frog)	spast	NP_001016453.1
	SPAST	XP_002799248.1
Canis lupus familiaris (dog)	SPAST	XP_850973.1
Drosophila melanogaster (fruit fly)	spas	NP_651206.3
Pan troglodytes (chimpanzee)	SPAST	XP_001164367.1
Mus musculus (house mouse)	Spast	NP_001156342.1
Rattus norvegicus (Norway rat)	Spast	NP_001102172.2
Danio rerio (zebrafish)	spast	NP_998080.1
Arabidopsis thaliana (thale cress)	AT2G45500	NP_182074.4
Bos taurus (cattle)	SPAST	NP_001075060.1
Gallus gallus (chicken)	SPAST	NP_001026232.1
Anopheles gambiae (African malaria mosquito)	SPAST_ANOGA	XP_312634.5
Caenorhabditis elegans (roundworm)	spas-1	NP_741586.1

Gene Ontology
Bibliography

Related articles in PubMed

Graded Control of Microtubule Severing by Tubulin Glutamylation.
Valenstein ML, Roll-Mecak A
Cell164(5)911-21(2016 Feb)

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
Vietri M, Schink KO, Campsteijn C, Wegner CS, Schultz SW, Christ L, Thoresen SB, Brech A, Raiborg C, Stenmark H
Nature522(7555)231-5(2015 Jun)

Spastin-interacting protein NA14/SSNA1 functions in cytokinesis and axon development.
Goyal U, Renvois? B, Chang J, Blackstone C
PloS one9(11)e112428(2014)

Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Chang J, Lee S, Blackstone C
Proceedings of the National Academy of Sciences of the United States of America110(37)14954-9(2013 Sep)

The nucleotide cycle of spastin correlates with its microtubule-binding properties.
Wen M, Wang C
The FEBS journal280(16)3868-77(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NA14 may act as an adaptor protein regulating SPG4 localization to centrosomes, temporally and spatially regulating the microtubule-severing activity of SPG4 that is particularly critical during the cell cycle and neuronal development.
Title: Spastin-interacting protein NA14/SSNA1 functions in cytokinesis and axon development.

The following Spast gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spast cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa39833	NM_001108702.2 Latest version!	Rattus norvegicus spastin (Spast), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
ORa48315	XM_008764486.2 Latest version!	Rattus norvegicus spastin (Spast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa48316	XM_008764487.2 Latest version!	Rattus norvegicus spastin (Spast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa48317	XM_008764488.2 Latest version!	Rattus norvegicus spastin (Spast), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spast ( NM_001108702.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102172.2 Rattus norvegicus spastin (Spast), mRNA.

Price & Availability↑

CloneID	ORa39833
Clone ID Related Accession (Same CDS sequence)	NM_001108702.2
Accession Version	NM_001108702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	spastin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC166846.1 and AC139392.4. On Dec 23, 2009 this sequence version replaced NM_001108702.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166846.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCAG GCGGCGCGAG CCCCGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGCCC 
GGCTCGCCGC ATAAGCGGAA CCTGTATTAC TTCTCGTATC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGACTCCAG CCCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTTGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGCATC GAAGAACTAG AGAAAGGAAT CGCGGTTATA 
GTTACGGGCC AAGGTGAACA GTACGAAAGA GCTAGACGCC TTCAAGCCAA AATGATGACG 
AATTTGGTTA TGGCTAAGGA CCGTTTACAA CTCCTAGAAA GTGGAGCAGT TCCGAAGAAG 
AAAGACCCCT TAACACATGC TAGTAATTCA TTGCCTCGAT CAAAAACTGT CATGAAAAGT 
GGATCCACAG GGCTCTCCGG TCACCACAGG GCGCCTAGTT GCAGTGGTTT ATCCATGGTT 
TCTGGAGCAA GACCGGGATC TGGTCCTGCA GCTACCACAC ATAAGGGTAC TTCAAAACCA 
AATAGAACCA ACAAACCTTC TACACCCACA ACTGCAGTTC GGAAAAAGAA AGACTTGAAA 
AATTTTAGGA ATGTGGACAG CAATCTTGCT AACCTTATAA TGAATGAAAT TGTTGACAAT 
GGGACAGCTG TTAAATTTGA TGATATAGCT GGTCAGGAAC TGGCAAAACA AGCATTGCAA 
GAGATTGTCA TTCTCCCTTC TCTGCGGCCT GAGTTGTTCA CAGGGCTCAG AGCTCCCGCC 
AGAGGCTTGT TACTCTTCGG TCCACCAGGA AACGGAAAAA CCATGCTGGC TAAAGCAGTA 
GCTGCAGAAT CTAATGCGAC TTTTTTCAAT ATAAGTGCTG CAAGTTTAAC TTCAAAATAT 
GTAGGAGAGG GAGAGAAATT GGTGAGAGCT CTTTTTGCTG TGGCTCGAGA ACTTCAACCC 
TCTATAATTT TTATAGATGA AGTTGATAGT CTTTTGTGTG AGAGAAGAGA AGGGGAGCAC 
GATGCTAGTA GACGACTAAA AACTGAATTT TTAATAGAAT TTGATGGTGT GCAGTCTGCT 
GGAGATGACA GAGTCCTCGT AATGGGTGCA ACCAACAGGC CCCAAGAGCT GGATGAAGCT 
GTTCTCAGGC GTTTCATTAA ACGGGTGTAT GTGTCATTAC CCAATGAGGA GACACGACTA 
CTTCTACTTA AAAACCTGTT ATGTAAACAA GGAAGTCCAC TGACCCAAAA AGAACTTGCT 
CAACTGGCGA GAATGACTGA TGGATACTCT GGAAGTGATC TGACTGCTTT GGCAAAGGAT 
GCAGCACTGG GTCCTATCCG AGAACTGAAA CCAGAGCAGG TGAAGAACAT GTCTGCCAGT 
GAGATGAGAA ATATTCGATT ATCTGACTTC ACAGAATCCT TAAAAAAGAT CAAACGCAGT 
GTGAGTCCTC AGACTTTAGA AGCATACATA CGCTGGAACA AGGACTTTGG AGACACCACT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102172.2
CDS	5..1750
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAPGSPHKRNLYYFSYPLVVGFALLRLLACHLGLLFVWLCQRFSRALMAAKRSSGTAPAPASPSTPAPGPGGEAESVRVFHKQAFEYISIALRIDEEEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLESGAVPKKKDPLTHASNSLPRSKTVMKSGSTGLSGHHRAPSCSGLSMVSGARPGSGPAATTHKGTSKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_001108702.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spastin (Spast), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108702.2

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCAG GCGGCGCGAG CCCCGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGCCC 
GGCTCGCCGC ATAAGCGGAA CCTGTATTAC TTCTCGTATC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGACTCCAG CCCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTTGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGCATC GAAGAACTAG AGAAAGGAAT CGCGGTTATA 
GTTACGGGCC AAGGTGAACA GTACGAAAGA GCTAGACGCC TTCAAGCCAA AATGATGACG 
AATTTGGTTA TGGCTAAGGA CCGTTTACAA CTCCTAGAAA GTGGAGCAGT TCCGAAGAAG 
AAAGACCCCT TAACACATGC TAGTAATTCA TTGCCTCGAT CAAAAACTGT CATGAAAAGT 
GGATCCACAG GGCTCTCCGG TCACCACAGG GCGCCTAGTT GCAGTGGTTT ATCCATGGTT 
TCTGGAGCAA GACCGGGATC TGGTCCTGCA GCTACCACAC ATAAGGGTAC TTCAAAACCA 
AATAGAACCA ACAAACCTTC TACACCCACA ACTGCAGTTC GGAAAAAGAA AGACTTGAAA 
AATTTTAGGA ATGTGGACAG CAATCTTGCT AACCTTATAA TGAATGAAAT TGTTGACAAT 
GGGACAGCTG TTAAATTTGA TGATATAGCT GGTCAGGAAC TGGCAAAACA AGCATTGCAA 
GAGATTGTCA TTCTCCCTTC TCTGCGGCCT GAGTTGTTCA CAGGGCTCAG AGCTCCCGCC 
AGAGGCTTGT TACTCTTCGG TCCACCAGGA AACGGAAAAA CCATGCTGGC TAAAGCAGTA 
GCTGCAGAAT CTAATGCGAC TTTTTTCAAT ATAAGTGCTG CAAGTTTAAC TTCAAAATAT 
GTAGGAGAGG GAGAGAAATT GGTGAGAGCT CTTTTTGCTG TGGCTCGAGA ACTTCAACCC 
TCTATAATTT TTATAGATGA AGTTGATAGT CTTTTGTGTG AGAGAAGAGA AGGGGAGCAC 
GATGCTAGTA GACGACTAAA AACTGAATTT TTAATAGAAT TTGATGGTGT GCAGTCTGCT 
GGAGATGACA GAGTCCTCGT AATGGGTGCA ACCAACAGGC CCCAAGAGCT GGATGAAGCT 
GTTCTCAGGC GTTTCATTAA ACGGGTGTAT GTGTCATTAC CCAATGAGGA GACACGACTA 
CTTCTACTTA AAAACCTGTT ATGTAAACAA GGAAGTCCAC TGACCCAAAA AGAACTTGCT 
CAACTGGCGA GAATGACTGA TGGATACTCT GGAAGTGATC TGACTGCTTT GGCAAAGGAT 
GCAGCACTGG GTCCTATCCG AGAACTGAAA CCAGAGCAGG TGAAGAACAT GTCTGCCAGT 
GAGATGAGAA ATATTCGATT ATCTGACTTC ACAGAATCCT TAAAAAAGAT CAAACGCAGT 
GTGAGTCCTC AGACTTTAGA AGCATACATA CGCTGGAACA AGGACTTTGG AGACACCACT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( XM_008764486.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762708.1 Rattus norvegicus spastin (Spast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa48315
Clone ID Related Accession (Same CDS sequence)	XM_008764486.2
Accession Version	XM_008764486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCAG GCGGCGCGAG CCCCGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGCCC 
GGCTCGCCGC ATAAGCGGAA CCTGTATTAC TTCTCGTATC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGACTCCAG CCCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTTGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGC ATCGAAGAAC TAGAGAAAGG AATCGCGGTT 
ATAGTTACGG GCCAAGGTGA ACAGTACGAA AGAGCTAGAC GCCTTCAAGC CAAAATGATG 
ACGAATTTGG TTATGGCTAA GGACCGTTTA CAACTCCTAG AGAAGCTGCA ACCAGTTTTG 
CAATTTTCCA AGTCACAAAC GGACGTCTAT AATGAGAGCA CTAACCTGAC ATGCCGCAAT 
GGACATCTCC AGTCAGAAAG TGGAGCAGTT CCGAAGAAGA AAGACCCCTT AACACATGCT 
AGTAATTCAT TGCCTCGATC AAAAACTGTC ATGAAAAGTG GATCCACAGG GCTCTCCGGT 
CACCACAGGG CGCCTAGTTG CAGTGGTTTA TCCATGGTTT CTGGAGCAAG ACCGGGATCT 
GGTCCTGCAG CTACCACACA TAAGGGTACT TCAAAACCAA ATAGAACCAA CAAACCTTCT 
ACACCCACAA CTGCAGTTCG GAAAAAGAAA GACTTGAAAA ATTTTAGGAA TGTGGACAGC 
AATCTTGCTA ACCTTATAAT GAATGAAATT GTTGACAATG GGACAGCTGT TAAATTTGAT 
GATATAGCTG GTCAGGAACT GGCAAAACAA GCATTGCAAG AGATTGTCAT TCTCCCTTCT 
CTGCGGCCTG AGTTGTTCAC AGGGCTCAGA GCTCCCGCCA GAGGCTTGTT ACTCTTCGGT 
CCACCAGGAA ACGGAAAAAC CATGCTGGCT AAAGCAGTAG CTGCAGAATC TAATGCGACT 
TTTTTCAATA TAAGTGCTGC AAGTTTAACT TCAAAATATG TAGGAGAGGG AGAGAAATTG 
GTGAGAGCTC TTTTTGCTGT GGCTCGAGAA CTTCAACCCT CTATAATTTT TATAGATGAA 
GTTGATAGTC TTTTGTGTGA GAGAAGAGAA GGGGAGCACG ATGCTAGTAG ACGACTAAAA 
ACTGAATTTT TAATAGAATT TGATGGTGTG CAGTCTGCTG GAGATGACAG AGTCCTCGTA 
ATGGGTGCAA CCAACAGGCC CCAAGAGCTG GATGAAGCTG TTCTCAGGCG TTTCATTAAA 
CGGGTGTATG TGTCATTACC CAATGAGGAG ACACGACTAC TTCTACTTAA AAACCTGTTA 
TGTAAACAAG GAAGTCCACT GACCCAAAAA GAACTTGCTC AACTGGCGAG AATGACTGAT 
GGATACTCTG GAAGTGATCT GACTGCTTTG GCAAAGGATG CAGCACTGGG TCCTATCCGA 
GAACTGAAAC CAGAGCAGGT GAAGAACATG TCTGCCAGTG AGATGAGAAA TATTCGATTA 
TCTGACTTCA CAGAATCCTT AAAAAAGATC AAACGCAGTG TGAGTCCTCA GACTTTAGAA 
GCATACATAC GCTGGAACAA GGACTTTGGA GACACCACTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762708.1
CDS	284..2128
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAPGSPHKRNLYYFSYPLVVGFALLRLLACHLGLLFVWLCQRFSRALMAAKRSSGTAPAPASPSTPAPGPGGEAESVRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFSKSQTDVYNESTNLTCRNGHLQSESGAVPKKKDPLTHASNSLPRSKTVMKSGSTGLSGHHRAPSCSGLSMVSGARPGSGPAATTHKGTSKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_008764486.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spastin (Spast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764486.2

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCAG GCGGCGCGAG CCCCGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGCCC 
GGCTCGCCGC ATAAGCGGAA CCTGTATTAC TTCTCGTATC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGACTCCAG CCCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTTGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGC ATCGAAGAAC TAGAGAAAGG AATCGCGGTT 
ATAGTTACGG GCCAAGGTGA ACAGTACGAA AGAGCTAGAC GCCTTCAAGC CAAAATGATG 
ACGAATTTGG TTATGGCTAA GGACCGTTTA CAACTCCTAG AGAAGCTGCA ACCAGTTTTG 
CAATTTTCCA AGTCACAAAC GGACGTCTAT AATGAGAGCA CTAACCTGAC ATGCCGCAAT 
GGACATCTCC AGTCAGAAAG TGGAGCAGTT CCGAAGAAGA AAGACCCCTT AACACATGCT 
AGTAATTCAT TGCCTCGATC AAAAACTGTC ATGAAAAGTG GATCCACAGG GCTCTCCGGT 
CACCACAGGG CGCCTAGTTG CAGTGGTTTA TCCATGGTTT CTGGAGCAAG ACCGGGATCT 
GGTCCTGCAG CTACCACACA TAAGGGTACT TCAAAACCAA ATAGAACCAA CAAACCTTCT 
ACACCCACAA CTGCAGTTCG GAAAAAGAAA GACTTGAAAA ATTTTAGGAA TGTGGACAGC 
AATCTTGCTA ACCTTATAAT GAATGAAATT GTTGACAATG GGACAGCTGT TAAATTTGAT 
GATATAGCTG GTCAGGAACT GGCAAAACAA GCATTGCAAG AGATTGTCAT TCTCCCTTCT 
CTGCGGCCTG AGTTGTTCAC AGGGCTCAGA GCTCCCGCCA GAGGCTTGTT ACTCTTCGGT 
CCACCAGGAA ACGGAAAAAC CATGCTGGCT AAAGCAGTAG CTGCAGAATC TAATGCGACT 
TTTTTCAATA TAAGTGCTGC AAGTTTAACT TCAAAATATG TAGGAGAGGG AGAGAAATTG 
GTGAGAGCTC TTTTTGCTGT GGCTCGAGAA CTTCAACCCT CTATAATTTT TATAGATGAA 
GTTGATAGTC TTTTGTGTGA GAGAAGAGAA GGGGAGCACG ATGCTAGTAG ACGACTAAAA 
ACTGAATTTT TAATAGAATT TGATGGTGTG CAGTCTGCTG GAGATGACAG AGTCCTCGTA 
ATGGGTGCAA CCAACAGGCC CCAAGAGCTG GATGAAGCTG TTCTCAGGCG TTTCATTAAA 
CGGGTGTATG TGTCATTACC CAATGAGGAG ACACGACTAC TTCTACTTAA AAACCTGTTA 
TGTAAACAAG GAAGTCCACT GACCCAAAAA GAACTTGCTC AACTGGCGAG AATGACTGAT 
GGATACTCTG GAAGTGATCT GACTGCTTTG GCAAAGGATG CAGCACTGGG TCCTATCCGA 
GAACTGAAAC CAGAGCAGGT GAAGAACATG TCTGCCAGTG AGATGAGAAA TATTCGATTA 
TCTGACTTCA CAGAATCCTT AAAAAAGATC AAACGCAGTG TGAGTCCTCA GACTTTAGAA 
GCATACATAC GCTGGAACAA GGACTTTGGA GACACCACTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( XM_008764487.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762709.1 Rattus norvegicus spastin (Spast), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa48316
Clone ID Related Accession (Same CDS sequence)	XM_008764487.2
Accession Version	XM_008764487.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spastin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCAG GCGGCGCGAG CCCCGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGCCC 
GGCTCGCCGC ATAAGCGGAA CCTGTATTAC TTCTCGTATC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGACTCCAG CCCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTTGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGCATC GAAGAACTAG AGAAAGGAAT CGCGGTTATA 
GTTACGGGCC AAGGTGAACA GTACGAAAGA GCTAGACGCC TTCAAGCCAA AATGATGACG 
AATTTGGTTA TGGCTAAGGA CCGTTTACAA CTCCTAGAGA AGCTGCAACC AGTTTTGCAA 
TTTTCCAAGT CACAAACGGA CGTCTATAAT GAGAGCACTA ACCTGACATG CCGCAATGGA 
CATCTCCAGT CAGAAAGTGG AGCAGTTCCG AAGAAGAAAG ACCCCTTAAC ACATGCTAGT 
AATTCATTGC CTCGATCAAA AACTGTCATG AAAAGTGGAT CCACAGGGCT CTCCGGTCAC 
CACAGGGCGC CTAGTTGCAG TGGTTTATCC ATGGTTTCTG GAGCAAGACC GGGATCTGGT 
CCTGCAGCTA CCACACATAA GGGTACTTCA AAACCAAATA GAACCAACAA ACCTTCTACA 
CCCACAACTG CAGTTCGGAA AAAGAAAGAC TTGAAAAATT TTAGGAATGT GGACAGCAAT 
CTTGCTAACC TTATAATGAA TGAAATTGTT GACAATGGGA CAGCTGTTAA ATTTGATGAT 
ATAGCTGGTC AGGAACTGGC AAAACAAGCA TTGCAAGAGA TTGTCATTCT CCCTTCTCTG 
CGGCCTGAGT TGTTCACAGG GCTCAGAGCT CCCGCCAGAG GCTTGTTACT CTTCGGTCCA 
CCAGGAAACG GAAAAACCAT GCTGGCTAAA GCAGTAGCTG CAGAATCTAA TGCGACTTTT 
TTCAATATAA GTGCTGCAAG TTTAACTTCA AAATATGTAG GAGAGGGAGA GAAATTGGTG 
AGAGCTCTTT TTGCTGTGGC TCGAGAACTT CAACCCTCTA TAATTTTTAT AGATGAAGTT 
GATAGTCTTT TGTGTGAGAG AAGAGAAGGG GAGCACGATG CTAGTAGACG ACTAAAAACT 
GAATTTTTAA TAGAATTTGA TGGTGTGCAG TCTGCTGGAG ATGACAGAGT CCTCGTAATG 
GGTGCAACCA ACAGGCCCCA AGAGCTGGAT GAAGCTGTTC TCAGGCGTTT CATTAAACGG 
GTGTATGTGT CATTACCCAA TGAGGAGACA CGACTACTTC TACTTAAAAA CCTGTTATGT 
AAACAAGGAA GTCCACTGAC CCAAAAAGAA CTTGCTCAAC TGGCGAGAAT GACTGATGGA 
TACTCTGGAA GTGATCTGAC TGCTTTGGCA AAGGATGCAG CACTGGGTCC TATCCGAGAA 
CTGAAACCAG AGCAGGTGAA GAACATGTCT GCCAGTGAGA TGAGAAATAT TCGATTATCT 
GACTTCACAG AATCCTTAAA AAAGATCAAA CGCAGTGTGA GTCCTCAGAC TTTAGAAGCA 
TACATACGCT GGAACAAGGA CTTTGGAGAC ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762709.1
CDS	284..2125
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAPGSPHKRNLYYFSYPLVVGFALLRLLACHLGLLFVWLCQRFSRALMAAKRSSGTAPAPASPSTPAPGPGGEAESVRVFHKQAFEYISIALRIDEEEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFSKSQTDVYNESTNLTCRNGHLQSESGAVPKKKDPLTHASNSLPRSKTVMKSGSTGLSGHHRAPSCSGLSMVSGARPGSGPAATTHKGTSKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_008764487.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spastin (Spast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764487.2

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCAG GCGGCGCGAG CCCCGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGCCC 
GGCTCGCCGC ATAAGCGGAA CCTGTATTAC TTCTCGTATC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGACTCCAG CCCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTTGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGCATC GAAGAACTAG AGAAAGGAAT CGCGGTTATA 
GTTACGGGCC AAGGTGAACA GTACGAAAGA GCTAGACGCC TTCAAGCCAA AATGATGACG 
AATTTGGTTA TGGCTAAGGA CCGTTTACAA CTCCTAGAGA AGCTGCAACC AGTTTTGCAA 
TTTTCCAAGT CACAAACGGA CGTCTATAAT GAGAGCACTA ACCTGACATG CCGCAATGGA 
CATCTCCAGT CAGAAAGTGG AGCAGTTCCG AAGAAGAAAG ACCCCTTAAC ACATGCTAGT 
AATTCATTGC CTCGATCAAA AACTGTCATG AAAAGTGGAT CCACAGGGCT CTCCGGTCAC 
CACAGGGCGC CTAGTTGCAG TGGTTTATCC ATGGTTTCTG GAGCAAGACC GGGATCTGGT 
CCTGCAGCTA CCACACATAA GGGTACTTCA AAACCAAATA GAACCAACAA ACCTTCTACA 
CCCACAACTG CAGTTCGGAA AAAGAAAGAC TTGAAAAATT TTAGGAATGT GGACAGCAAT 
CTTGCTAACC TTATAATGAA TGAAATTGTT GACAATGGGA CAGCTGTTAA ATTTGATGAT 
ATAGCTGGTC AGGAACTGGC AAAACAAGCA TTGCAAGAGA TTGTCATTCT CCCTTCTCTG 
CGGCCTGAGT TGTTCACAGG GCTCAGAGCT CCCGCCAGAG GCTTGTTACT CTTCGGTCCA 
CCAGGAAACG GAAAAACCAT GCTGGCTAAA GCAGTAGCTG CAGAATCTAA TGCGACTTTT 
TTCAATATAA GTGCTGCAAG TTTAACTTCA AAATATGTAG GAGAGGGAGA GAAATTGGTG 
AGAGCTCTTT TTGCTGTGGC TCGAGAACTT CAACCCTCTA TAATTTTTAT AGATGAAGTT 
GATAGTCTTT TGTGTGAGAG AAGAGAAGGG GAGCACGATG CTAGTAGACG ACTAAAAACT 
GAATTTTTAA TAGAATTTGA TGGTGTGCAG TCTGCTGGAG ATGACAGAGT CCTCGTAATG 
GGTGCAACCA ACAGGCCCCA AGAGCTGGAT GAAGCTGTTC TCAGGCGTTT CATTAAACGG 
GTGTATGTGT CATTACCCAA TGAGGAGACA CGACTACTTC TACTTAAAAA CCTGTTATGT 
AAACAAGGAA GTCCACTGAC CCAAAAAGAA CTTGCTCAAC TGGCGAGAAT GACTGATGGA 
TACTCTGGAA GTGATCTGAC TGCTTTGGCA AAGGATGCAG CACTGGGTCC TATCCGAGAA 
CTGAAACCAG AGCAGGTGAA GAACATGTCT GCCAGTGAGA TGAGAAATAT TCGATTATCT 
GACTTCACAG AATCCTTAAA AAAGATCAAA CGCAGTGTGA GTCCTCAGAC TTTAGAAGCA 
TACATACGCT GGAACAAGGA CTTTGGAGAC ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( XM_008764488.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762710.1 Rattus norvegicus spastin (Spast), transcript variant X3, mRNA.

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CloneID	ORa48317
Clone ID Related Accession (Same CDS sequence)	XM_008764488.2
Accession Version	XM_008764488.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spastin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCAG GCGGCGCGAG CCCCGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGCCC 
GGCTCGCCGC ATAAGCGGAA CCTGTATTAC TTCTCGTATC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGACTCCAG CCCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTTGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGC ATCGAAGAAC TAGAGAAAGG AATCGCGGTT 
ATAGTTACGG GCCAAGGTGA ACAGTACGAA AGAGCTAGAC GCCTTCAAGC CAAAATGATG 
ACGAATTTGG TTATGGCTAA GGACCGTTTA CAACTCCTAG AAAGTGGAGC AGTTCCGAAG 
AAGAAAGACC CCTTAACACA TGCTAGTAAT TCATTGCCTC GATCAAAAAC TGTCATGAAA 
AGTGGATCCA CAGGGCTCTC CGGTCACCAC AGGGCGCCTA GTTGCAGTGG TTTATCCATG 
GTTTCTGGAG CAAGACCGGG ATCTGGTCCT GCAGCTACCA CACATAAGGG TACTTCAAAA 
CCAAATAGAA CCAACAAACC TTCTACACCC ACAACTGCAG TTCGGAAAAA GAAAGACTTG 
AAAAATTTTA GGAATGTGGA CAGCAATCTT GCTAACCTTA TAATGAATGA AATTGTTGAC 
AATGGGACAG CTGTTAAATT TGATGATATA GCTGGTCAGG AACTGGCAAA ACAAGCATTG 
CAAGAGATTG TCATTCTCCC TTCTCTGCGG CCTGAGTTGT TCACAGGGCT CAGAGCTCCC 
GCCAGAGGCT TGTTACTCTT CGGTCCACCA GGAAACGGAA AAACCATGCT GGCTAAAGCA 
GTAGCTGCAG AATCTAATGC GACTTTTTTC AATATAAGTG CTGCAAGTTT AACTTCAAAA 
TATGTAGGAG AGGGAGAGAA ATTGGTGAGA GCTCTTTTTG CTGTGGCTCG AGAACTTCAA 
CCCTCTATAA TTTTTATAGA TGAAGTTGAT AGTCTTTTGT GTGAGAGAAG AGAAGGGGAG 
CACGATGCTA GTAGACGACT AAAAACTGAA TTTTTAATAG AATTTGATGG TGTGCAGTCT 
GCTGGAGATG ACAGAGTCCT CGTAATGGGT GCAACCAACA GGCCCCAAGA GCTGGATGAA 
GCTGTTCTCA GGCGTTTCAT TAAACGGGTG TATGTGTCAT TACCCAATGA GGAGACACGA 
CTACTTCTAC TTAAAAACCT GTTATGTAAA CAAGGAAGTC CACTGACCCA AAAAGAACTT 
GCTCAACTGG CGAGAATGAC TGATGGATAC TCTGGAAGTG ATCTGACTGC TTTGGCAAAG 
GATGCAGCAC TGGGTCCTAT CCGAGAACTG AAACCAGAGC AGGTGAAGAA CATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACAGAAT CCTTAAAAAA GATCAAACGC 
AGTGTGAGTC CTCAGACTTT AGAAGCATAC ATACGCTGGA ACAAGGACTT TGGAGACACC 
ACTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762710.1
CDS	285..2033
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAPGSPHKRNLYYFSYPLVVGFALLRLLACHLGLLFVWLCQRFSRALMAAKRSSGTAPAPASPSTPAPGPGGEAESVRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLESGAVPKKKDPLTHASNSLPRSKTVMKSGSTGLSGHHRAPSCSGLSMVSGARPGSGPAATTHKGTSKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_008764488.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spastin (Spast), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764488.2

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCAG GCGGCGCGAG CCCCGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGCCC 
GGCTCGCCGC ATAAGCGGAA CCTGTATTAC TTCTCGTATC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGACTCCAG CCCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTTGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGC ATCGAAGAAC TAGAGAAAGG AATCGCGGTT 
ATAGTTACGG GCCAAGGTGA ACAGTACGAA AGAGCTAGAC GCCTTCAAGC CAAAATGATG 
ACGAATTTGG TTATGGCTAA GGACCGTTTA CAACTCCTAG AAAGTGGAGC AGTTCCGAAG 
AAGAAAGACC CCTTAACACA TGCTAGTAAT TCATTGCCTC GATCAAAAAC TGTCATGAAA 
AGTGGATCCA CAGGGCTCTC CGGTCACCAC AGGGCGCCTA GTTGCAGTGG TTTATCCATG 
GTTTCTGGAG CAAGACCGGG ATCTGGTCCT GCAGCTACCA CACATAAGGG TACTTCAAAA 
CCAAATAGAA CCAACAAACC TTCTACACCC ACAACTGCAG TTCGGAAAAA GAAAGACTTG 
AAAAATTTTA GGAATGTGGA CAGCAATCTT GCTAACCTTA TAATGAATGA AATTGTTGAC 
AATGGGACAG CTGTTAAATT TGATGATATA GCTGGTCAGG AACTGGCAAA ACAAGCATTG 
CAAGAGATTG TCATTCTCCC TTCTCTGCGG CCTGAGTTGT TCACAGGGCT CAGAGCTCCC 
GCCAGAGGCT TGTTACTCTT CGGTCCACCA GGAAACGGAA AAACCATGCT GGCTAAAGCA 
GTAGCTGCAG AATCTAATGC GACTTTTTTC AATATAAGTG CTGCAAGTTT AACTTCAAAA 
TATGTAGGAG AGGGAGAGAA ATTGGTGAGA GCTCTTTTTG CTGTGGCTCG AGAACTTCAA 
CCCTCTATAA TTTTTATAGA TGAAGTTGAT AGTCTTTTGT GTGAGAGAAG AGAAGGGGAG 
CACGATGCTA GTAGACGACT AAAAACTGAA TTTTTAATAG AATTTGATGG TGTGCAGTCT 
GCTGGAGATG ACAGAGTCCT CGTAATGGGT GCAACCAACA GGCCCCAAGA GCTGGATGAA 
GCTGTTCTCA GGCGTTTCAT TAAACGGGTG TATGTGTCAT TACCCAATGA GGAGACACGA 
CTACTTCTAC TTAAAAACCT GTTATGTAAA CAAGGAAGTC CACTGACCCA AAAAGAACTT 
GCTCAACTGG CGAGAATGAC TGATGGATAC TCTGGAAGTG ATCTGACTGC TTTGGCAAAG 
GATGCAGCAC TGGGTCCTAT CCGAGAACTG AAACCAGAGC AGGTGAAGAA CATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACAGAAT CCTTAAAAAA GATCAAACGC 
AGTGTGAGTC CTCAGACTTT AGAAGCATAC ATACGCTGGA ACAAGGACTT TGGAGACACC 
ACTGTTTAA