Lamtor3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lamtor3
Entrez Gene ID	362045
Full Name	late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
Synonyms	MP1,Map2k1ip1,Mapksp1
General protein information	Preferred Names late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 Names ragulator complex protein LAMTOR3 MEK partner 1 late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 mitogen-activated protein kinase kinase 1-interacting protein 1 mitogen-activated protein kinase scaffold protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233385.3	XP_006233447.1	ragulator complex protein LAMTOR3 isoform X1
NM_001008375.1	NP_001008376.1	ragulator complex protein LAMTOR3
XM_006233383.3	XP_006233445.1	ragulator complex protein LAMTOR3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LAMTOR3	NP_068805.1
Canis lupus familiaris (dog)	LAMTOR3	XP_862361.1
Rattus norvegicus (Norway rat)	Lamtor3	NP_001008376.1
Xenopus tropicalis (tropical clawed frog)	lamtor3	NP_001135566.1
Pan troglodytes (chimpanzee)	LAMTOR3	XP_517362.2
Bos taurus (cattle)	LAMTOR3	NP_001069450.1
Mus musculus (house mouse)	Lamtor3	NP_064304.1
Danio rerio (zebrafish)	lamtor3	NP_001018410.1
	MAPKSP1	XP_001107957.1
Gallus gallus (chicken)	LAMTOR3	NP_001006559.1
Drosophila melanogaster (fruit fly)	CG5110	NP_001246062.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000997	XP_309192.2

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of mTORC1 by amino acids.
Bar-Peled L, Sabatini DM
Trends in cell biology24(7)400-6(2014 Jul)

Ragulator is a GEF for the rag GTPases that signal amino acid levels to mTORC1.
Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
Cell150(6)1196-208(2012 Sep)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids.
Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM
Cell141(2)290-303(2010 Apr)

The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK-ERK pathway to late endosomes.
Nada S, Hondo A, Kasai A, Koike M, Saito K, Uchiyama Y, Okada M
The EMBO journal28(5)477-89(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The MP1/p14 complex regulates PAK1-dependent ERK activation during adhesion and cell spreading but is not required for ERK activation by platelet-derived growth factor.
Title: The MEK1 scaffolding protein MP1 regulates cell spreading by integrating PAK1 and Rho signals.

The following Lamtor3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lamtor3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11945	NM_001008375.1 Latest version!	Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa11945	XM_006233385.3 Latest version!	Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa11945	XM_006233383.3 Latest version!	Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lamtor3 ( NM_001008375.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008376.1 Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), mRNA.

Price & Availability↑

CloneID	ORa11945
Clone ID Related Accession (Same CDS sequence)	XM_006233385.3 , NM_001008375.1 , XM_006233383.3
Accession Version	NM_001008375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	ragulator complex protein LAMTOR3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086353.1. On Dec 16, 2004 this sequence version replaced XM_342347.1. ##Evidence-Data-START## Transcript exon combination :: BC086353.1, EV764509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATG ATCTAAAGCG ATTCCTATAC AAAAAGTTGC CAAGCGTTGA AGGGCTCCAT 
GCAATCGTTG TGTCAGATAG AGATGGGGTG CCTGTTATTA AAGTGGCTAA CGACAGTGCT 
CCAGAACATG CCCTGAGGCC TGGCTTCTTG TCCACTTTCG CCCTCGCAAC AGACCAAGGC 
AGCAAGCTCG GACTTTCAAA AAATAAAAGC ATCATCTGCT ACTATAATAC CTACCAGGTG 
GTTCAATTCA ATCGTTTGCC TCTGGTGGTG AGTTTTATAG CCAGCAGCAA TGCCAACACA 
GGACTAATTG TCAGCCTAGA AAAGGAGCTC GCTCCCTTAT TTGAAGAACT GATAAAAGTT 
GTGGAAGTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008376.1
CDS	119..493
Translation	MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDSAPEHALRPGFLSTFALATDQGSKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSNANTGLIVSLEKELAPLFEELIKVVEVS

Target ORF information:

RefSeq Version	NM_001008375.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008375.1

ORF Insert Sequence:

ATGGCGGATG ATCTAAAGCG ATTCCTATAC AAAAAGTTGC CAAGCGTTGA AGGGCTCCAT 
GCAATCGTTG TGTCAGATAG AGATGGGGTG CCTGTTATTA AAGTGGCTAA CGACAGTGCT 
CCAGAACATG CCCTGAGGCC TGGCTTCTTG TCCACTTTCG CCCTCGCAAC AGACCAAGGC 
AGCAAGCTCG GACTTTCAAA AAATAAAAGC ATCATCTGCT ACTATAATAC CTACCAGGTG 
GTTCAATTCA ATCGTTTGCC TCTGGTGGTG AGTTTTATAG CCAGCAGCAA TGCCAACACA 
GGACTAATTG TCAGCCTAGA AAAGGAGCTC GCTCCCTTAT TTGAAGAACT GATAAAAGTT 
GTGGAAGTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lamtor3 ( XM_006233385.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233447.1 Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa11945
Clone ID Related Accession (Same CDS sequence)	XM_006233385.3 , NM_001008375.1 , XM_006233383.3
Accession Version	XM_006233385.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ragulator complex protein LAMTOR3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG ATCTAAAGCG ATTCCTATAC AAAAAGTTGC CAAGCGTTGA AGGGCTCCAT 
GCAATCGTTG TGTCAGATAG AGATGGGGTG CCTGTTATTA AAGTGGCTAA CGACAGTGCT 
CCAGAACATG CCCTGAGGCC TGGCTTCTTG TCCACTTTCG CCCTCGCAAC AGACCAAGGC 
AGCAAGCTCG GACTTTCAAA AAATAAAAGC ATCATCTGCT ACTATAATAC CTACCAGGTG 
GTTCAATTCA ATCGTTTGCC TCTGGTGGTG AGTTTTATAG CCAGCAGCAA TGCCAACACA 
GGACTAATTG TCAGCCTAGA AAAGGAGCTC GCTCCCTTAT TTGAAGAACT GATAAAAGTT 
GTGGAAGTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233447.1
CDS	189..563
Translation	MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDSAPEHALRPGFLSTFALATDQGSKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSNANTGLIVSLEKELAPLFEELIKVVEVS

Target ORF information:

RefSeq Version	XM_006233385.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233385.3

ORF Insert Sequence:

ATGGCGGATG ATCTAAAGCG ATTCCTATAC AAAAAGTTGC CAAGCGTTGA AGGGCTCCAT 
GCAATCGTTG TGTCAGATAG AGATGGGGTG CCTGTTATTA AAGTGGCTAA CGACAGTGCT 
CCAGAACATG CCCTGAGGCC TGGCTTCTTG TCCACTTTCG CCCTCGCAAC AGACCAAGGC 
AGCAAGCTCG GACTTTCAAA AAATAAAAGC ATCATCTGCT ACTATAATAC CTACCAGGTG 
GTTCAATTCA ATCGTTTGCC TCTGGTGGTG AGTTTTATAG CCAGCAGCAA TGCCAACACA 
GGACTAATTG TCAGCCTAGA AAAGGAGCTC GCTCCCTTAT TTGAAGAACT GATAAAAGTT 
GTGGAAGTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lamtor3 ( XM_006233383.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233445.1 Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11945
Clone ID Related Accession (Same CDS sequence)	XM_006233385.3 , NM_001008375.1 , XM_006233383.3
Accession Version	XM_006233383.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ragulator complex protein LAMTOR3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233383.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG ATCTAAAGCG ATTCCTATAC AAAAAGTTGC CAAGCGTTGA AGGGCTCCAT 
GCAATCGTTG TGTCAGATAG AGATGGGGTG CCTGTTATTA AAGTGGCTAA CGACAGTGCT 
CCAGAACATG CCCTGAGGCC TGGCTTCTTG TCCACTTTCG CCCTCGCAAC AGACCAAGGC 
AGCAAGCTCG GACTTTCAAA AAATAAAAGC ATCATCTGCT ACTATAATAC CTACCAGGTG 
GTTCAATTCA ATCGTTTGCC TCTGGTGGTG AGTTTTATAG CCAGCAGCAA TGCCAACACA 
GGACTAATTG TCAGCCTAGA AAAGGAGCTC GCTCCCTTAT TTGAAGAACT GATAAAAGTT 
GTGGAAGTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233445.1
CDS	244..618
Translation	MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDSAPEHALRPGFLSTFALATDQGSKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSNANTGLIVSLEKELAPLFEELIKVVEVS

Target ORF information:

RefSeq Version	XM_006233383.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233383.3

ORF Insert Sequence:

ATGGCGGATG ATCTAAAGCG ATTCCTATAC AAAAAGTTGC CAAGCGTTGA AGGGCTCCAT 
GCAATCGTTG TGTCAGATAG AGATGGGGTG CCTGTTATTA AAGTGGCTAA CGACAGTGCT 
CCAGAACATG CCCTGAGGCC TGGCTTCTTG TCCACTTTCG CCCTCGCAAC AGACCAAGGC 
AGCAAGCTCG GACTTTCAAA AAATAAAAGC ATCATCTGCT ACTATAATAC CTACCAGGTG 
GTTCAATTCA ATCGTTTGCC TCTGGTGGTG AGTTTTATAG CCAGCAGCAA TGCCAACACA 
GGACTAATTG TCAGCCTAGA AAAGGAGCTC GCTCCCTTAT TTGAAGAACT GATAAAAGTT 
GTGGAAGTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of mTORC1 by amino acids.
Trends in cell biology24(7)400-6(2014 Jul)
Bar-Peled L,Sabatini DM

Ragulator is a GEF for the rag GTPases that signal amino acid levels to mTORC1.
Cell150(6)1196-208(2012 Sep)
Bar-Peled L,Schweitzer LD,Zoncu R,Sabatini DM

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids.
Cell141(2)290-303(2010 Apr)
Sancak Y,Bar-Peled L,Zoncu R,Markhard AL,Nada S,Sabatini DM

The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK-ERK pathway to late endosomes.
The EMBO journal28(5)477-89(2009 Mar)
Nada S,Hondo A,Kasai A,Koike M,Saito K,Uchiyama Y,Okada M