Ect2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ect2
Entrez Gene ID	361921
Full Name	epithelial cell transforming 2
General protein information	Preferred Names epithelial cell transforming 2 Names protein ECT2 ect2 oncogene epithelial cell transforming sequence 2 oncogene
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108547.2	NP_001102017.2	protein ECT2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ECT2	XP_003950278.1
	ECT2	XP_001083830.1
Gallus gallus (chicken)	ECT2	XP_004943527.1
Danio rerio (zebrafish)	ect2	NP_001003883.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005796	XP_556459.3
Homo sapiens (human)	ECT2	NP_001245244.1
Bos taurus (cattle)	ECT2	NP_001091042.1
Rattus norvegicus (Norway rat)	Ect2	NP_001102017.2
Drosophila melanogaster (fruit fly)	pbl	NP_523965.2
Mus musculus (house mouse)	Ect2	NP_031926.2
Canis lupus familiaris (dog)	ECT2	XP_005639979.1
Xenopus tropicalis (tropical clawed frog)	ect2	NP_001106617.1

Gene Ontology
Bibliography

Related articles in PubMed

Annexin V-induced rat Leydig cell proliferation involves Ect2 via RhoA/ROCK signaling pathway.
Jing J, Chen L, Fu HY, Fan K, Yao Q, Ge YF, Lu JC, Yao B
Scientific reports59437(2015 Mar)

Ect2, an ortholog of Drosophila's pebble, negatively regulates neurite outgrowth in neuroblastoma ? glioma hybrid NG108-15 cells.
Tsuji T, Higashida C, Yoshida Y, Islam MS, Dohmoto M, Koizumi K, Higashida H
Cellular and molecular neurobiology31(5)663-8(2011 Jul)

The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage.
Srougi MC, Burridge K
PloS one6(2)e17108(2011 Feb)

Downregulation of cough by exercise and voluntary hyperpnea.
Fontana GA
Lung188 Suppl 1S95-8(2010 Jan)

Ect2 links the PKCiota-Par6alpha complex to Rac1 activation and cellular transformation.
Justilien V, Fields AP
Oncogene28(41)3597-607(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We thus conclude that Ect2 is involved in annexin V-induced rat Leydig cell proliferation through the RhoA/ROCK pathway
Title: Annexin V-induced rat Leydig cell proliferation involves Ect2 via RhoA/ROCK signaling pathway.

The following Ect2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ect2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13799	NM_001108547.2 Latest version!	Rattus norvegicus epithelial cell transforming 2 (Ect2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ect2 ( NM_001108547.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102017.2 Rattus norvegicus epithelial cell transforming 2 (Ect2), mRNA.

Price & Availability↑

CloneID	ORa13799
Clone ID Related Accession (Same CDS sequence)	NM_001108547.2
Accession Version	NM_001108547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Rattus norvegicus(Norway rat)
Product	protein ECT2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01015906.1. On Sep 8, 2012 this sequence version replaced NM_001108547.1. Sequence Note: Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGACG ACAGTGTGTT ACCATCTCCT ACTGAGATAA CTAGCTTGGC AGACACTTCA 
GTTTTTGATT CTAAAGTTAC AGAAACATCC AAGGAAAACT TGTGCATGGT GTCTACTTCA 
AATGTTGACG AAGAAATGCC TCAGGTGGAA GCAAGAGTGG TAGTGGTCCA GGAAGCTGGG 
AAACAAGAGG AGCTCCTAAA GGCCTTAAAG ACTGTTAAGA TAATGGAAGT CCCTGTTATA 
AAGATAAAAG AAAGTTGTCC TGGGAAATCG GATGAAAAGT TAATAAAAAG TATTATTAAT 
ATGGAAATTA AAGTGCCCTG TGTAAAGATG GATTCAATGG AAGAATTTGG AAGTTTGGAT 
TCCCCAGAAT TTGAGAATAT ATTTGTAGTT ACTGACTTCC AGACATCTGT CTTCAATGAC 
TTATACAAGG CTGATTGTAG GATTGTTGGA CCACCAGTTA TACTCAACTG TGCACAAATG 
GGAGAGCCCT TGCCGTTCTC CTGCCGGCCG CTGTACTGCA CGAGCATGCT GAATCTGGTG 
CTCTGCTTCA CTGGATTCAG GAAGAAGGAG GAGCTCGTCA AATTGGTGAC ATTGGTTCAT 
CATATGGGTG GAGTTATTCG AAAAGAATGC AATTCCAAAG TAACACATCT GGTGGCAAAT 
TGTACGCAAG GAGAAAAGTT TAGGGTTGCT GTGAGCCTGG GGACTCCAAT TATGAGGCCA 
GGATGGATTT ATGAAGCATG GGAAAGACGC AATGAACAGT GTTTCTGTGC AGCAGATGAT 
GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTCCAAGATT GCATTTTAAG TTTTCTGGGA 
TTTTCAGATG AAGAGAAACA CAGTATGGAA GAAATGACTG AAATGCAAGG AGGTACCTAT 
TTACCAGTTG GGGATGAAAG GTGCACTCAC CTTATTGTTG ATGAGAACAC AGTAAAGGAA 
CTTCCATTTG AACCTTCCAA GAAACTTTTT GTGGTCAAGC AAGAGTGGTT CTGGGGAAGC 
ATTCAGATGG ATGCCCGGGC TGGTGAAACT ATGTATTTGT ATGAAAAGGC TAATACTCCT 
GAGCTCAAGA AATCCGTGTC TTTGCTGTCT CTGAGTACTC CAAACAGCAA CCGCAAAAGA 
CGGAGGTTGA AAGAAAGCCT GGCTCAGCTC TCCAGAGAGA CCGACCTCTC TCCTTTCCCT 
CCGCGCAAGC GGCCCTCAGC AGAGCATTCC CTTTCTATCG GCTCGCTCCT GGATATCTCC 
AACACACCGG ACTCCAGCAT TCACTATGGA GAAACACCGA AGTCTTGTGC TAAGTCTTCC 
AGGAGTTCAA CTCCAGTTCC TCCAAAGCAG TCGGCCAGGT GGCAGGTGGC AAAAGAGCTC 
TACCAGACTG AGAGCAACTA TGTCAATATA CTGGCCACCA TCATTCAATT ATTTCAGGTA 
CCCTTAGAAG AAGAAGGACA ACGGGGTGGG CCTATTCTTG CACCCGAGGA AATCAAGACT 
ATTTTTGGGA GCATCCCAGA CATCTTTGAC GTACATATGA AGATAAAGGA TGATCTTGAA 
GACCTTATTG CTAACTGGGA TGAGAGCAGA AGTATTGGTG ACATCTTTCT TAAATATTCC 
AAAGATTTGG TAAAAACCTA TCCTCCATTT GTAAACTTCT TTGAAATGAG CAAGGAAATG 
ATTATTAAAT GTGAAAAACA GAAGCCCAGA TTTCATGCTT TTCTCAAGAT AAATCAAGCT 
AAACCAGAGT GTGGACGGCA AAGCCTTGTG GAACTTCTCA TTCGGCCAGT ACAGCGGTTA 
CCCAGTGTTG CCTTACTTTT AAACGATCTT AAAAAGCATA CAGCTGATGA AAATCCAGAC 
AAAAGCACGT TAGAAAAAGC TATTGGATCA CTAAAAGAAG TAATGACACA TATCAATGAG 
GATAAGAGAA AAACAGAAGC ACAGAAGCAA ATTTTTGATG TTGTTTATGA AGTAGATGGA 
TGCCCAGCTA ATCTATTATC TTCTCATCGA AGCCTGATAC AAAGAGTGGA AACAGTTTCT 
CTTGGTGAGC ACCCCTGTGA CCGAGGAGAA CAAGTCACTC TCTTCCTCTT CAATGACTGC 
CTTGAGATAG CAAGAAAACG GCACAAGGTT ATTGGCACTT TTAGAAGTCC TCATGACCGC 
ACCCGGCCAC CAGCTTCTCT GAAGCACATT CATCTAATGC CTCTTTCTCA GATTAAGAAG 
GTGCTGGACA TCAGAGAGAC AGAAGATTGT CATAATGCCT TTGCCTTGCT TGTGAGGCCA 
CCAACAGAGC AGGCCAACGT GCTGCTCAGC TTCCAGATGA CGTCAGAGGA GCTTCCAAAG 
GAGAGCTGGC TGAAGATGCT GTGTCGACAT GTTGCCAACA CCATTTGTAA AGCAGATGCT 
GAGAATCTTA TGTACGTGAC TGATCCAGAA TCTTTTGAAG TAAATACAAA AGATATGGAT 
AGTACACTTA GTCGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACAAGGGCA 
TTCTCTTTTT CCAAAACTCC CAAGAGAGCC CTGCGGATGG CTCTGTCATC ATCCCATAGC 
TCAGAGGGAA GGAGTCCTCC GAGTAGTGGC AAACTTGCTG TGAGCCGTCT GTCCAGCACA 
TCTTCCTTAG CAGGTATTCC ATCGCCTTCC CTTGTCAGCC TCCCTTCTTT CTTTGAACGG 
AGAAGTCATA CATTAAGTCG ATCTACAACT CACTTGATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102017.2
CDS	137..2878
Translation	MADDSVLPSPTEITSLADTSVFDSKVTETSKENLCMVSTSNVDEEMPQVEARVVVVQEAGKQEELLKALKTVKIMEVPVIKIKESCPGKSDEKLIKSIINMEIKVPCVKMDSMEEFGSLDSPEFENIFVVTDFQTSVFNDLYKADCRIVGPPVILNCAQMGEPLPFSCRPLYCTSMLNLVLCFTGFRKKEELVKLVTLVHHMGGVIRKECNSKVTHLVANCTQGEKFRVAVSLGTPIMRPGWIYEAWERRNEQCFCAADDDFRNEFKVPPFQDCILSFLGFSDEEKHSMEEMTEMQGGTYLPVGDERCTHLIVDENTVKELPFEPSKKLFVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSLLSLSTPNSNRKRRRLKESLAQLSRETDLSPFPPRKRPSAEHSLSIGSLLDISNTPDSSIHYGETPKSCAKSSRSSTPVPPKQSARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHMKIKDDLEDLIANWDESRSIGDIFLKYSKDLVKTYPPFVNFFEMSKEMIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLIQRVETVSLGEHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHDRTRPPASLKHIHLMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSEELPKESWLKMLCRHVANTICKADAENLMYVTDPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRMALSSSHSSEGRSPPSSGKLAVSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI

Target ORF information:

RefSeq Version	NM_001108547.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus epithelial cell transforming 2 (Ect2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108547.2

ORF Insert Sequence:

ATGGCGGACG ACAGTGTGTT ACCATCTCCT ACTGAGATAA CTAGCTTGGC AGACACTTCA 
GTTTTTGATT CTAAAGTTAC AGAAACATCC AAGGAAAACT TGTGCATGGT GTCTACTTCA 
AATGTTGACG AAGAAATGCC TCAGGTGGAA GCAAGAGTGG TAGTGGTCCA GGAAGCTGGG 
AAACAAGAGG AGCTCCTAAA GGCCTTAAAG ACTGTTAAGA TAATGGAAGT CCCTGTTATA 
AAGATAAAAG AAAGTTGTCC TGGGAAATCG GATGAAAAGT TAATAAAAAG TATTATTAAT 
ATGGAAATTA AAGTGCCCTG TGTAAAGATG GATTCAATGG AAGAATTTGG AAGTTTGGAT 
TCCCCAGAAT TTGAGAATAT ATTTGTAGTT ACTGACTTCC AGACATCTGT CTTCAATGAC 
TTATACAAGG CTGATTGTAG GATTGTTGGA CCACCAGTTA TACTCAACTG TGCACAAATG 
GGAGAGCCCT TGCCGTTCTC CTGCCGGCCG CTGTACTGCA CGAGCATGCT GAATCTGGTG 
CTCTGCTTCA CTGGATTCAG GAAGAAGGAG GAGCTCGTCA AATTGGTGAC ATTGGTTCAT 
CATATGGGTG GAGTTATTCG AAAAGAATGC AATTCCAAAG TAACACATCT GGTGGCAAAT 
TGTACGCAAG GAGAAAAGTT TAGGGTTGCT GTGAGCCTGG GGACTCCAAT TATGAGGCCA 
GGATGGATTT ATGAAGCATG GGAAAGACGC AATGAACAGT GTTTCTGTGC AGCAGATGAT 
GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTCCAAGATT GCATTTTAAG TTTTCTGGGA 
TTTTCAGATG AAGAGAAACA CAGTATGGAA GAAATGACTG AAATGCAAGG AGGTACCTAT 
TTACCAGTTG GGGATGAAAG GTGCACTCAC CTTATTGTTG ATGAGAACAC AGTAAAGGAA 
CTTCCATTTG AACCTTCCAA GAAACTTTTT GTGGTCAAGC AAGAGTGGTT CTGGGGAAGC 
ATTCAGATGG ATGCCCGGGC TGGTGAAACT ATGTATTTGT ATGAAAAGGC TAATACTCCT 
GAGCTCAAGA AATCCGTGTC TTTGCTGTCT CTGAGTACTC CAAACAGCAA CCGCAAAAGA 
CGGAGGTTGA AAGAAAGCCT GGCTCAGCTC TCCAGAGAGA CCGACCTCTC TCCTTTCCCT 
CCGCGCAAGC GGCCCTCAGC AGAGCATTCC CTTTCTATCG GCTCGCTCCT GGATATCTCC 
AACACACCGG ACTCCAGCAT TCACTATGGA GAAACACCGA AGTCTTGTGC TAAGTCTTCC 
AGGAGTTCAA CTCCAGTTCC TCCAAAGCAG TCGGCCAGGT GGCAGGTGGC AAAAGAGCTC 
TACCAGACTG AGAGCAACTA TGTCAATATA CTGGCCACCA TCATTCAATT ATTTCAGGTA 
CCCTTAGAAG AAGAAGGACA ACGGGGTGGG CCTATTCTTG CACCCGAGGA AATCAAGACT 
ATTTTTGGGA GCATCCCAGA CATCTTTGAC GTACATATGA AGATAAAGGA TGATCTTGAA 
GACCTTATTG CTAACTGGGA TGAGAGCAGA AGTATTGGTG ACATCTTTCT TAAATATTCC 
AAAGATTTGG TAAAAACCTA TCCTCCATTT GTAAACTTCT TTGAAATGAG CAAGGAAATG 
ATTATTAAAT GTGAAAAACA GAAGCCCAGA TTTCATGCTT TTCTCAAGAT AAATCAAGCT 
AAACCAGAGT GTGGACGGCA AAGCCTTGTG GAACTTCTCA TTCGGCCAGT ACAGCGGTTA 
CCCAGTGTTG CCTTACTTTT AAACGATCTT AAAAAGCATA CAGCTGATGA AAATCCAGAC 
AAAAGCACGT TAGAAAAAGC TATTGGATCA CTAAAAGAAG TAATGACACA TATCAATGAG 
GATAAGAGAA AAACAGAAGC ACAGAAGCAA ATTTTTGATG TTGTTTATGA AGTAGATGGA 
TGCCCAGCTA ATCTATTATC TTCTCATCGA AGCCTGATAC AAAGAGTGGA AACAGTTTCT 
CTTGGTGAGC ACCCCTGTGA CCGAGGAGAA CAAGTCACTC TCTTCCTCTT CAATGACTGC 
CTTGAGATAG CAAGAAAACG GCACAAGGTT ATTGGCACTT TTAGAAGTCC TCATGACCGC 
ACCCGGCCAC CAGCTTCTCT GAAGCACATT CATCTAATGC CTCTTTCTCA GATTAAGAAG 
GTGCTGGACA TCAGAGAGAC AGAAGATTGT CATAATGCCT TTGCCTTGCT TGTGAGGCCA 
CCAACAGAGC AGGCCAACGT GCTGCTCAGC TTCCAGATGA CGTCAGAGGA GCTTCCAAAG 
GAGAGCTGGC TGAAGATGCT GTGTCGACAT GTTGCCAACA CCATTTGTAA AGCAGATGCT 
GAGAATCTTA TGTACGTGAC TGATCCAGAA TCTTTTGAAG TAAATACAAA AGATATGGAT 
AGTACACTTA GTCGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACAAGGGCA 
TTCTCTTTTT CCAAAACTCC CAAGAGAGCC CTGCGGATGG CTCTGTCATC ATCCCATAGC 
TCAGAGGGAA GGAGTCCTCC GAGTAGTGGC AAACTTGCTG TGAGCCGTCT GTCCAGCACA 
TCTTCCTTAG CAGGTATTCC ATCGCCTTCC CTTGTCAGCC TCCCTTCTTT CTTTGAACGG 
AGAAGTCATA CATTAAGTCG ATCTACAACT CACTTGATAT GA