Bscl2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bscl2
Entrez Gene ID	361722
Full Name	BSCL2, seipin lipid droplet biogenesis associated
General protein information	Preferred Names BSCL2, seipin lipid droplet biogenesis associated Names seipin Berardinelli-Seip congenital lipodystrophy 2 (seipin) bernardinelli-Seip congenital lipodystrophy type 2 protein homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012171.1	NP_001012171.1	seipin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BSCL2	XP_853538.1
Mus musculus (house mouse)	Bscl2	NP_001129536.1
	BSCL2	XP_002799560.1
Danio rerio (zebrafish)	bscl2	NP_001032473.1
Homo sapiens (human)	BSCL2	NP_001116427.1
Pan troglodytes (chimpanzee)	LOC100609080	XP_003313147.1
Rattus norvegicus (Norway rat)	Bscl2	NP_001012171.1
Bos taurus (cattle)	BSCL2	NP_001015589.1

Gene Ontology
Bibliography

Related articles in PubMed

Seipin deficiency alters brown adipose tissue thermogenesis and insulin sensitivity in a non-cell autonomous mode.
Dollet L, Magr? J, Joubert M, Le May C, Ayer A, Arnaud L, Pecqueur C, Blouin V, Cariou B, Prieur X
Scientific reports635487(2016 Oct)

Seipin is necessary for normal brain development and spermatogenesis in addition to adipogenesis.
Ebihara C, Ebihara K, Aizawa-Abe M, Mashimo T, Tomita T, Zhao M, Gumbilai V, Kusakabe T, Yamamoto Y, Aotani D, Yamamoto-Kataoka S, Sakai T, Hosoda K, Serikawa T, Nakao K
Human molecular genetics24(15)4238-49(2015 Aug)

Adipose-specific knockout of SEIPIN/BSCL2 results in progressive lipodystrophy.
Liu L, Jiang Q, Wang X, Zhang Y, Lin RC, Lam SM, Shui G, Zhou L, Li P, Wang Y, Cui X, Gao M, Zhang L, Lv Y, Xu G, Liu G, Zhao D, Yang H
Diabetes63(7)2320-31(2014 Jul)

Lack of testicular seipin causes teratozoospermia syndrome in men.
Jiang M, Gao M, Wu C, He H, Guo X, Zhou Z, Yang H, Xiao X, Liu G, Sha J
Proceedings of the National Academy of Sciences of the United States of America111(19)7054-9(2014 May)

Seipin differentially regulates lipogenesis and adipogenesis through a conserved core sequence and an evolutionarily acquired C-terminus.
Yang W, Thein S, Guo X, Xu F, Venkatesh B, Sugii S, Radda GK, Han W
The Biochemical journal452(1)37-44(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we generated a Bscl2/seipin knockout (SKO) rat using the method with ENU (N-ethyl-N-nitrosourea) mutagenesis. SKO rats showed total lack of white adipose tissues including mechanical fat such as bone marrow and retro-orbital fats
Title: Seipin is necessary for normal brain development and spermatogenesis in addition to adipogenesis.

Localization, cDNA sequence and genomic organization of the rat and sequence analysis in inbred rat models of Type 2 diabetes mellitus.
Title: Localization, cDNA sequence and genomic organization of the rat seipin gene (Bscl2) and sequence analysis in inbred rat models of Type 2 diabetes mellitus.

The following Bscl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bscl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02555	NM_001012171.1 Latest version!	Rattus norvegicus BSCL2 lipid droplet biogenesis associated, seipin (Bscl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bscl2 ( NM_001012171.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012171.1 Rattus norvegicus BSCL2 lipid droplet biogenesis associated, seipin (Bscl2), mRNA.

Price & Availability↑

CloneID	ORa02555
Clone ID Related Accession (Same CDS sequence)	NM_001012171.1
Accession Version	NM_001012171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	seipin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089942.1. On Feb 11, 2005 this sequence version replaced XM_342013.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089942.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTCAATG ACCCACCAGT CCCTGCCTTG CTGTGGGCCC AGGAAGTGGG CCATGTGTTG 
GCAGGCCGCG CCCGCAGGCT GATGCTGCAG TTTGGGGTGC TCTTTTGTAC CATCCTCCTT 
TTGCTTTGGG TGTCTGTGTT CCTCTACGGT TCTTTCTACT ACTCCTACAT GCCGACCGTC 
AGCCACCTCA GCCCTGTGCA TTTCTATTAC AGGACAGACT GTGATTCCTC TACTGCTTCG 
CTCTGCTCTT TCCCTGTTGC CAATGTCTCG CTGACTAAGA GTGGACGTGA CCGGGTGCTG 
ATGTATGGAC AGCCATATCG AGTCACCTTA GAGCTTGAGC TGCCAGAATC CCCTGTGAAT 
CAAGACTTGG GCATGTTCTT AGTCACCGTT TCGTGCTATA CCAGAGGTGG CCGAATCATC 
TCCACTTCTT CACGCTCGGT GATGCTTCAT TACCGCTCTC AGCTGCTACA GATGCTCGAC 
ACGCTGGTGT TCTCCAGCCT GCTGCTGTTT GGCTTTGCAG AACAGAAGCA GCTCCTGGAA 
GTAGAACTCT ACTCTGACTA TAGGGAGAAT TCGTATGTAC CAACAACTGG GGCAATTATC 
GAAGTCCACA GCAAGCGTGT CCAGATGTAT GGAGCTTACC TCCGCATCCA TGCCCACTTC 
ACTGGACTCA GGTACCTGCT ATACAACTTC CCCATGACCT GCGCCTTCGT GGGTGTGGCC 
AGCAACTTCA CATTCCTCAG TGTCATCGCG CTCTTCAGCT ATATGCAGTG GGTGTGGGGT 
GCTGTCTGGC CCCGACACCG CTTCTCTTTG CAGGTTAATA TCCGACAAAG GGATAATTCA 
GGACACGGGG CCCAGCGTCG GATCTCTCGC CATCAGCCAG GTCAGGCGTC TACCCAGCAG 
TCAGATGTGA CAGAGGATGG CGAGAGCCCT GAGGATCCCT CAGGAACAGA GGGTCAGCTG 
TCTGAGGAAG AGAAACCAGA GAAGCAGCCT CTGAATGGAG AAGGGGAGCA GGAGCCAGAG 
GCTAGTGACG GCTCCTGGGA AGATGCTGCT TTGCTGACCG AAGCCTCAAC GTCTGCCCTC 
GCCCCTGAGA CTCTGGGCAG CCTCAGGCAA CGCCAGACCT GCTCCAGTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012171.1
CDS	390..1523
Translation	MVNDPPVPALLWAQEVGHVLAGRARRLMLQFGVLFCTILLLLWVSVFLYGSFYYSYMPTVSHLSPVHFYYRTDCDSSTASLCSFPVANVSLTKSGRDRVLMYGQPYRVTLELELPESPVNQDLGMFLVTVSCYTRGGRIISTSSRSVMLHYRSQLLQMLDTLVFSSLLLFGFAEQKQLLEVELYSDYRENSYVPTTGAIIEVHSKRVQMYGAYLRIHAHFTGLRYLLYNFPMTCAFVGVASNFTFLSVIALFSYMQWVWGAVWPRHRFSLQVNIRQRDNSGHGAQRRISRHQPGQASTQQSDVTEDGESPEDPSGTEGQLSEEEKPEKQPLNGEGEQEPEASDGSWEDAALLTEASTSALAPETLGSLRQRQTCSSS

Target ORF information:

RefSeq Version	NM_001012171.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BSCL2 lipid droplet biogenesis associated, seipin (Bscl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012171.1

ORF Insert Sequence:

ATGGTCAATG ACCCACCAGT CCCTGCCTTG CTGTGGGCCC AGGAAGTGGG CCATGTGTTG 
GCAGGCCGCG CCCGCAGGCT GATGCTGCAG TTTGGGGTGC TCTTTTGTAC CATCCTCCTT 
TTGCTTTGGG TGTCTGTGTT CCTCTACGGT TCTTTCTACT ACTCCTACAT GCCGACCGTC 
AGCCACCTCA GCCCTGTGCA TTTCTATTAC AGGACAGACT GTGATTCCTC TACTGCTTCG 
CTCTGCTCTT TCCCTGTTGC CAATGTCTCG CTGACTAAGA GTGGACGTGA CCGGGTGCTG 
ATGTATGGAC AGCCATATCG AGTCACCTTA GAGCTTGAGC TGCCAGAATC CCCTGTGAAT 
CAAGACTTGG GCATGTTCTT AGTCACCGTT TCGTGCTATA CCAGAGGTGG CCGAATCATC 
TCCACTTCTT CACGCTCGGT GATGCTTCAT TACCGCTCTC AGCTGCTACA GATGCTCGAC 
ACGCTGGTGT TCTCCAGCCT GCTGCTGTTT GGCTTTGCAG AACAGAAGCA GCTCCTGGAA 
GTAGAACTCT ACTCTGACTA TAGGGAGAAT TCGTATGTAC CAACAACTGG GGCAATTATC 
GAAGTCCACA GCAAGCGTGT CCAGATGTAT GGAGCTTACC TCCGCATCCA TGCCCACTTC 
ACTGGACTCA GGTACCTGCT ATACAACTTC CCCATGACCT GCGCCTTCGT GGGTGTGGCC 
AGCAACTTCA CATTCCTCAG TGTCATCGCG CTCTTCAGCT ATATGCAGTG GGTGTGGGGT 
GCTGTCTGGC CCCGACACCG CTTCTCTTTG CAGGTTAATA TCCGACAAAG GGATAATTCA 
GGACACGGGG CCCAGCGTCG GATCTCTCGC CATCAGCCAG GTCAGGCGTC TACCCAGCAG 
TCAGATGTGA CAGAGGATGG CGAGAGCCCT GAGGATCCCT CAGGAACAGA GGGTCAGCTG 
TCTGAGGAAG AGAAACCAGA GAAGCAGCCT CTGAATGGAG AAGGGGAGCA GGAGCCAGAG 
GCTAGTGACG GCTCCTGGGA AGATGCTGCT TTGCTGACCG AAGCCTCAAC GTCTGCCCTC 
GCCCCTGAGA CTCTGGGCAG CCTCAGGCAA CGCCAGACCT GCTCCAGTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Seipin deficiency alters brown adipose tissue thermogenesis and insulin sensitivity in a non-cell autonomous mode.
Scientific reports635487(2016 Oct)
Dollet L,Magr? J,Joubert M,Le May C,Ayer A,Arnaud L,Pecqueur C,Blouin V,Cariou B,Prieur X

Seipin is necessary for normal brain development and spermatogenesis in addition to adipogenesis.
Human molecular genetics24(15)4238-49(2015 Aug)
Ebihara C,Ebihara K,Aizawa-Abe M,Mashimo T,Tomita T,Zhao M,Gumbilai V,Kusakabe T,Yamamoto Y,Aotani D,Yamamoto-Kataoka S,Sakai T,Hosoda K,Serikawa T,Nakao K

Adipose-specific knockout of SEIPIN/BSCL2 results in progressive lipodystrophy.
Diabetes63(7)2320-31(2014 Jul)
Liu L,Jiang Q,Wang X,Zhang Y,Lin RC,Lam SM,Shui G,Zhou L,Li P,Wang Y,Cui X,Gao M,Zhang L,Lv Y,Xu G,Liu G,Zhao D,Yang H

Lack of testicular seipin causes teratozoospermia syndrome in men.
Proceedings of the National Academy of Sciences of the United States of America111(19)7054-9(2014 May)
Jiang M,Gao M,Wu C,He H,Guo X,Zhou Z,Yang H,Xiao X,Liu G,Sha J

Seipin differentially regulates lipogenesis and adipogenesis through a conserved core sequence and an evolutionarily acquired C-terminus.
The Biochemical journal452(1)37-44(2013 May)
Yang W,Thein S,Guo X,Xu F,Venkatesh B,Sugii S,Radda GK,Han W