Lcmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lcmt1
Entrez Gene ID	361643
Full Name	leucine carboxyl methyltransferase 1
General protein information	Preferred Names leucine carboxyl methyltransferase 1 Names leucine carboxyl methyltransferase 1 [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q36
Summary	human homolog is a carboxyl methyltransferase; involved in the methylation of catalytic subunit of protein phosphatase 2A [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006230191.3	XP_006230253.1	leucine carboxyl methyltransferase 1 isoform X1
NM_199405.2	NP_955437.1	leucine carboxyl methyltransferase 1

Pathways from BioSystems

Homologies

	LCMT1	XP_001099127.1
Drosophila melanogaster (fruit fly)	CG3793	NP_609766.1
Caenorhabditis elegans (roundworm)	CELE_B0285.4	NP_497875.1
Oryza sativa (rice)	Os06g0140100	NP_001056749.1
Xenopus tropicalis (tropical clawed frog)	lcmt1	NP_001016033.1
Pan troglodytes (chimpanzee)	LCMT1	XP_001164891.1
Canis lupus familiaris (dog)	LCMT1	XP_005621453.1
Bos taurus (cattle)	LCMT1	NP_001069956.1
Mus musculus (house mouse)	Lcmt1	NP_079580.2
Gallus gallus (chicken)	LCMT1	NP_001006167.2
Arabidopsis thaliana (thale cress)	AT1G02100	NP_973741.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008768	XP_314892.4
Homo sapiens (human)	LCMT1	NP_057393.2
Rattus norvegicus (Norway rat)	Lcmt1	NP_955437.1
Danio rerio (zebrafish)	lcmt1	NP_001004645.1

Gene Ontology
Bibliography

Related articles in PubMed

Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling.
MacKay KB, Tu Y, Young SG, Clarke SG
PloS one8(6)e65967(2013)

The structural basis for tight control of PP2A methylation and function by LCMT-1.
Stanevich V, Jiang L, Satyshur KA, Li Y, Jeffrey PD, Li Z, Menden P, Semmelhack MF, Xing Y
Molecular cell41(3)331-42(2011 Feb)

Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells.
Lee JA, Pallas DC
The Journal of biological chemistry282(42)30974-84(2007 Oct)

Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue.
De Baere I, Derua R, Janssens V, Van Hoof C, Waelkens E, Merlevede W, Goris J
Biochemistry38(50)16539-47(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A dominant-negative LCMT-1 mutant attenuates the cell cycle without causing cell death, likely by inhibiting uncontrolled phosphatase activity
Title: The structural basis for tight control of PP2A methylation and function by LCMT-1.

The following Lcmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lcmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05340	NM_199405.2 Latest version!	Rattus norvegicus leucine carboxyl methyltransferase 1 (Lcmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa23391	XM_006230191.3 Latest version!	Rattus norvegicus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lcmt1 ( NM_199405.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_955437.1 Rattus norvegicus leucine carboxyl methyltransferase 1 (Lcmt1), mRNA.

Price & Availability↑

CloneID	ORa05340
Clone ID Related Accession (Same CDS sequence)	NM_199405.2
Accession Version	NM_199405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	leucine carboxyl methyltransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC063186.1. On Aug 30, 2012 this sequence version replaced NM_199405.1. ##Evidence-Data-START## Transcript exon combination :: BC063186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACCG GCTCGAGGGA GTCCTCGTTT TCTTCCTGCG CGTCGAGCTG TGACTTTGAC 
GACGAGGGAG TGCGCGGCAC CTGCGAAGAT GCTTCTCTCT GCAAGAGGTT TGCGGTGAGC 
ATTGGCCACT GGCATGATCC ATACATCGAA CATCTTGTGA GGCAGTCTAA AGAGAGGAAG 
GCCCCTGAAA TTAACAGAGG ATATTTTGCT CGTGTTCATG GTGTCAGTCA GCTTATTAAA 
GCTTTTCTCC GGAAGACAGA ATGCCGATGT CAAATTCTCA ACCTTGGGGC TGGGATGGAT 
ACCACCTTCT GGAAATTAAA GGATGAAGGT CTTCTCCCAA ATAAATATTT TGAAGTTGAC 
TTTCCAATGA TAGTCACAAG AAAACTGCAC ACCATCAAAA ACAAGCCACT TCTATTCAGA 
CCCATCATGG AGTTACACCC AGAGGATACT CTTCAAATAG ATAGTCACAT GCTGGATTCA 
AAAAGATATG CCATCATTGG AGCAGATCTC CGAGACCTTT CTGAGCTGGA AGAGAAACTA 
AAGAAATGTA ACATGAATAC ACAGTTGCCC ACACTCCTGA TAACCGAATG TGTGCTGGTG 
TACATGACTC CGGAGCAGTC AGCCAACCTC CTAAAGTGGG CAGCCCGCAG TTTTGAGACG 
GCCATGTTCA TAAATTATGA ACAGGTCAAC ATGGATGACC GGTTTGGGCA GATCATGATT 
GAAAACCTCC GGAGGCGACA GTGTGACCTG GCAGGAGTGG AAACCTGCAA ATCACTGGAG 
TCTCAGAAGG AACGACTGCT GTTGAATGGG TGGGAGACGG CGTCTGCAGT GAACATGATG 
GAGCTGTACA GTGGGCTTCC TCGGGCAGAG GTAAACAGAA TAGAATCCCT CGAGTTCCTG 
GATGAAATGG AGCTTCTAGA GCAGCTCATG CGGCATTACT GCCTGTGCTG GGCCACCCGA 
GGGGGCCAGG AGCTGGGTTT GAAGGAGATA ACCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955437.1
CDS	112..1110
Translation	MATGSRESSFSSCASSCDFDDEGVRGTCEDASLCKRFAVSIGHWHDPYIEHLVRQSKERKAPEINRGYFARVHGVSQLIKAFLRKTECRCQILNLGAGMDTTFWKLKDEGLLPNKYFEVDFPMIVTRKLHTIKNKPLLFRPIMELHPEDTLQIDSHMLDSKRYAIIGADLRDLSELEEKLKKCNMNTQLPTLLITECVLVYMTPEQSANLLKWAARSFETAMFINYEQVNMDDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLLNGWETASAVNMMELYSGLPRAEVNRIESLEFLDEMELLEQLMRHYCLCWATRGGQELGLKEITY

Target ORF information:

RefSeq Version	NM_199405.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine carboxyl methyltransferase 1 (Lcmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199405.2

ORF Insert Sequence:

ATGGCCACCG GCTCGAGGGA GTCCTCGTTT TCTTCCTGCG CGTCGAGCTG TGACTTTGAC 
GACGAGGGAG TGCGCGGCAC CTGCGAAGAT GCTTCTCTCT GCAAGAGGTT TGCGGTGAGC 
ATTGGCCACT GGCATGATCC ATACATCGAA CATCTTGTGA GGCAGTCTAA AGAGAGGAAG 
GCCCCTGAAA TTAACAGAGG ATATTTTGCT CGTGTTCATG GTGTCAGTCA GCTTATTAAA 
GCTTTTCTCC GGAAGACAGA ATGCCGATGT CAAATTCTCA ACCTTGGGGC TGGGATGGAT 
ACCACCTTCT GGAAATTAAA GGATGAAGGT CTTCTCCCAA ATAAATATTT TGAAGTTGAC 
TTTCCAATGA TAGTCACAAG AAAACTGCAC ACCATCAAAA ACAAGCCACT TCTATTCAGA 
CCCATCATGG AGTTACACCC AGAGGATACT CTTCAAATAG ATAGTCACAT GCTGGATTCA 
AAAAGATATG CCATCATTGG AGCAGATCTC CGAGACCTTT CTGAGCTGGA AGAGAAACTA 
AAGAAATGTA ACATGAATAC ACAGTTGCCC ACACTCCTGA TAACCGAATG TGTGCTGGTG 
TACATGACTC CGGAGCAGTC AGCCAACCTC CTAAAGTGGG CAGCCCGCAG TTTTGAGACG 
GCCATGTTCA TAAATTATGA ACAGGTCAAC ATGGATGACC GGTTTGGGCA GATCATGATT 
GAAAACCTCC GGAGGCGACA GTGTGACCTG GCAGGAGTGG AAACCTGCAA ATCACTGGAG 
TCTCAGAAGG AACGACTGCT GTTGAATGGG TGGGAGACGG CGTCTGCAGT GAACATGATG 
GAGCTGTACA GTGGGCTTCC TCGGGCAGAG GTAAACAGAA TAGAATCCCT CGAGTTCCTG 
GATGAAATGG AGCTTCTAGA GCAGCTCATG CGGCATTACT GCCTGTGCTG GGCCACCCGA 
GGGGGCCAGG AGCTGGGTTT GAAGGAGATA ACCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lcmt1 ( XM_006230191.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230253.1 Rattus norvegicus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23391
Clone ID Related Accession (Same CDS sequence)	XM_006230191.3
Accession Version	XM_006230191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	leucine carboxyl methyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG GCTCGAGGGA GTCCTCGTTT TCTTCCTGCG CGTCGAGCTG TGACTTTGAC 
GACGAGGGAG TGCGCGGCAC CTGCGAAGAT GCTTCTCTCT GCAAGAGGTT TGCGGTGAGC 
ATTGGCTACT GGCATGATCC ATACATCGAA CATCTTGTGA GGCAGTCTAA AGAGAGGAAG 
GCCCCTGAAA TTAACAGAGG ATATTTTGCT CGTGTTCATG GTGTCAGTCA GCTTATTAAA 
GCTTTTCTCC GGAAGACAGA ATGCCGTTGT CAAATTCTCA ACCTTGGGGC TGGGATGGAT 
ACCACCTTCT GGAAATTAAA GGATGAAGGT CTTCTCCCAA ATAAATATTT TGAAGTTGAC 
TTTCCAATGA TAGTCACAAG AAAACTGCAC ACCATCAAAA ACAAGCCACT TCTATTCAGA 
CCCATCATGG AGTTACACCC AGAGGATACT CTTCAAATAG ATAGTCACAT GCTGGATTCA 
AAAAGATATG CCATCATTGG AGCAGATCTC CGAGACCTTT CTGAGCTGGA AGAGAAACTA 
AAGAAATGTA ACATGAATAC ACAGTTGCCC ACACTCCTGA TAACCGAATG TGTGCTGGTG 
TACATGACTC CGGAGCAGTC AGCCAACCTC CTAAAGTGGG CAGCCCGCAG TTTTGAGACG 
GCCATGTTCA TAAATTATGA ACAGGTCAAC ATGGATGACC GGTTTGGGCA GATCATGATT 
GAAAACCTCC GGAGGCGACA GTGTGACCTG GCAGGAGTGG AAACCTGCAA ATCACTGGAG 
TCTCAGAAGG AACGACTGCT GTTGAATGGG TGGGAGACGG CGTCTGCAGT GAACATGATG 
GAGCTGTACA GTGGGCTTCC TCGGGCAGAG GTAAACAGGT TTGAAGGAGA TAACCTATTG 
ATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230253.1
CDS	152..1060
Translation	MATGSRESSFSSCASSCDFDDEGVRGTCEDASLCKRFAVSIGYWHDPYIEHLVRQSKERKAPEINRGYFARVHGVSQLIKAFLRKTECRCQILNLGAGMDTTFWKLKDEGLLPNKYFEVDFPMIVTRKLHTIKNKPLLFRPIMELHPEDTLQIDSHMLDSKRYAIIGADLRDLSELEEKLKKCNMNTQLPTLLITECVLVYMTPEQSANLLKWAARSFETAMFINYEQVNMDDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLLNGWETASAVNMMELYSGLPRAEVNRFEGDNLLIC

Target ORF information:

RefSeq Version	XM_006230191.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230191.3

ORF Insert Sequence:

ATGGCCACCG GCTCGAGGGA GTCCTCGTTT TCTTCCTGCG CGTCGAGCTG TGACTTTGAC 
GACGAGGGAG TGCGCGGCAC CTGCGAAGAT GCTTCTCTCT GCAAGAGGTT TGCGGTGAGC 
ATTGGCTACT GGCATGATCC ATACATCGAA CATCTTGTGA GGCAGTCTAA AGAGAGGAAG 
GCCCCTGAAA TTAACAGAGG ATATTTTGCT CGTGTTCATG GTGTCAGTCA GCTTATTAAA 
GCTTTTCTCC GGAAGACAGA ATGCCGTTGT CAAATTCTCA ACCTTGGGGC TGGGATGGAT 
ACCACCTTCT GGAAATTAAA GGATGAAGGT CTTCTCCCAA ATAAATATTT TGAAGTTGAC 
TTTCCAATGA TAGTCACAAG AAAACTGCAC ACCATCAAAA ACAAGCCACT TCTATTCAGA 
CCCATCATGG AGTTACACCC AGAGGATACT CTTCAAATAG ATAGTCACAT GCTGGATTCA 
AAAAGATATG CCATCATTGG AGCAGATCTC CGAGACCTTT CTGAGCTGGA AGAGAAACTA 
AAGAAATGTA ACATGAATAC ACAGTTGCCC ACACTCCTGA TAACCGAATG TGTGCTGGTG 
TACATGACTC CGGAGCAGTC AGCCAACCTC CTAAAGTGGG CAGCCCGCAG TTTTGAGACG 
GCCATGTTCA TAAATTATGA ACAGGTCAAC ATGGATGACC GGTTTGGGCA GATCATGATT 
GAAAACCTCC GGAGGCGACA GTGTGACCTG GCAGGAGTGG AAACCTGCAA ATCACTGGAG 
TCTCAGAAGG AACGACTGCT GTTGAATGGG TGGGAGACGG CGTCTGCAGT GAACATGATG 
GAGCTGTACA GTGGGCTTCC TCGGGCAGAG GTAAACAGGT TTGAAGGAGA TAACCTATTG 
ATCTGCTGA