Tyrobp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tyrobp
Entrez Gene ID	361537
Full Name	Tyro protein tyrosine kinase binding protein
Synonyms	Karap
General protein information	Preferred Names Tyro protein tyrosine kinase binding protein Names TYRO protein tyrosine kinase-binding protein DAP12 DNAX-activation protein 12 killer cell activating receptor-associated protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_212525.1	NP_997690.1	TYRO protein tyrosine kinase-binding protein precursor
XM_008759166.2	XP_008757388.1	TYRO protein tyrosine kinase-binding protein isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYROBP	NP_003323.1
	TYROBP	NP_001028039.1
Canis lupus familiaris (dog)	TYROBP	NP_001184046.1
Bos taurus (cattle)	TYROBP	NP_777052.1
Mus musculus (house mouse)	Tyrobp	NP_035792.1
Pan troglodytes (chimpanzee)	TYROBP	NP_001077092.1
Rattus norvegicus (Norway rat)	Tyrobp	NP_997690.1

Gene Ontology
Bibliography

Related articles in PubMed

A DAP12-dependent signal promotes pro-inflammatory polarization in microglia following nerve injury and exacerbates degeneration of injured neurons.
Kobayashi M, Konishi H, Takai T, Kiyama H
Glia63(6)1073-82(2015 Jun)

DAP12 Stabilizes the C-terminal Fragment of the Triggering Receptor Expressed on Myeloid Cells-2 (TREM2) and Protects against LPS-induced Pro-inflammatory Response.
Zhong L, Chen XF, Zhang ZL, Wang Z, Shi XZ, Xu K, Zhang YW, Xu H, Bu G
The Journal of biological chemistry290(25)15866-77(2015 Jun)

DAP12 impacts trafficking and surface stability of killer immunoglobulin-like receptors on natural killer cells.
Mulrooney TJ, Posch PE, Hurley CK
Journal of leukocyte biology94(2)301-13(2013 Aug)

Siglec-15 protein regulates formation of functional osteoclasts in concert with DNAX-activating protein of 12 kDa (DAP12).
Ishida-Kitagawa N, Tanaka K, Bao X, Kimura T, Miura T, Kitaoka Y, Hayashi K, Sato M, Maruoka M, Ogawa T, Miyoshi J, Takeya T
The Journal of biological chemistry287(21)17493-502(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both DAP12 and DAP10 can associate with rat CD94/NKG2C. Surprisingly, DAP12 and DAP10 do not associate with NKG2C but instead with CD94.
Title: Rat and mouse CD94 associate directly with the activating transmembrane adaptor proteins DAP12 and DAP10 and activate NK cell cytotoxicity.

The following Tyrobp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tyrobp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05806	NM_212525.1 Latest version!	Rattus norvegicus transmembrane immune signaling adaptor Tyrobp (Tyrobp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa47941	XM_008759166.2 Latest version!	Rattus norvegicus Tyro protein tyrosine kinase binding protein (Tyrobp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tyrobp ( NM_212525.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997690.1 Rattus norvegicus transmembrane immune signaling adaptor Tyrobp (Tyrobp), mRNA.

Price & Availability↑

CloneID	ORa05806
Clone ID Related Accession (Same CDS sequence)	NM_212525.1
Accession Version	NM_212525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Rattus norvegicus(Norway rat)
Product	TYRO protein tyrosine kinase-binding protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY247021.1. On Aug 30, 2004 this sequence version replaced XM_341823.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY247021.1, FQ211365.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGCCC CAGAGCCCTC CTGGTGCTTT CTGTTCCTTC CTGTCCTCCT GACTGTGGGA 
GGATTAAGTC CCGTACAGGC CCAGAGTGAC AATTACCCAG GATGCGAATG TTCCTCTGTG 
AGCCCGGGTG TACTGGCTGG GATTGTGCTG GGCGACTTGG TGCTGACTCT GCTCATCGCC 
CTGGCGGTGT ACTCTCTGGG CCGCCTGGTC TCTCGAGGCC GAGGGACTGC AGACGGGACC 
CGGAAACAGC ACATGGCTGA GACTGAGTCA CCTTATCAGG AGCTTCAGGG TCAGAGGCCA 
GAAGTATACA GTGACCTCAA CACACAGAGG CAGTATTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997690.1
CDS	10..354
Translation	MGAPEPSWCFLFLPVLLTVGGLSPVQAQSDNYPGCECSSVSPGVLAGIVLGDLVLTLLIALAVYSLGRLVSRGRGTADGTRKQHMAETESPYQELQGQRPEVYSDLNTQRQYYR

Target ORF information:

RefSeq Version	NM_212525.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transmembrane immune signaling adaptor Tyrobp (Tyrobp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212525.1

ORF Insert Sequence:

ATGGGGGCCC CAGAGCCCTC CTGGTGCTTT CTGTTCCTTC CTGTCCTCCT GACTGTGGGA 
GGATTAAGTC CCGTACAGGC CCAGAGTGAC AATTACCCAG GATGCGAATG TTCCTCTGTG 
AGCCCGGGTG TACTGGCTGG GATTGTGCTG GGCGACTTGG TGCTGACTCT GCTCATCGCC 
CTGGCGGTGT ACTCTCTGGG CCGCCTGGTC TCTCGAGGCC GAGGGACTGC AGACGGGACC 
CGGAAACAGC ACATGGCTGA GACTGAGTCA CCTTATCAGG AGCTTCAGGG TCAGAGGCCA 
GAAGTATACA GTGACCTCAA CACACAGAGG CAGTATTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyrobp ( XM_008759166.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757388.1 Rattus norvegicus Tyro protein tyrosine kinase binding protein (Tyrobp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47941
Clone ID Related Accession (Same CDS sequence)	XM_008759166.2
Accession Version	XM_008759166.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	TYRO protein tyrosine kinase-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCC CAGAGCCCTC CTGGTGCTTT CTGTTCCTTC CTGTCCTCCT GACTGTGGGA 
GGATTAAGTC CCGTACAGGC CCAGAGTGGA TGCGAATGTT CCTCTGTGAG CCCGGGTGTA 
CTGGCTGGGA TTGTGCTGGG CGACTTGGTG CTGACTCTGC TCATCGCCCT GGCGGTGTAC 
TCTCTGGGCC GCCTGGTCTC TCGAGGCCGA GGGACTGCAG ACGGGACCCG GAAACAGCAC 
ATGGCTGAGA CTGAGTCACC TTATCAGGAG CTTCAGGGTC AGAGGCCAGA AGTATACAGT 
GACCTCAACA CACAGAGGCA GTATTACAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757388.1
CDS	89..421
Translation	MGAPEPSWCFLFLPVLLTVGGLSPVQAQSGCECSSVSPGVLAGIVLGDLVLTLLIALAVYSLGRLVSRGRGTADGTRKQHMAETESPYQELQGQRPEVYSDLNTQRQYYR

Target ORF information:

RefSeq Version	XM_008759166.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Tyro protein tyrosine kinase binding protein (Tyrobp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759166.2

ORF Insert Sequence:

ATGGGGGCCC CAGAGCCCTC CTGGTGCTTT CTGTTCCTTC CTGTCCTCCT GACTGTGGGA 
GGATTAAGTC CCGTACAGGC CCAGAGTGGA TGCGAATGTT CCTCTGTGAG CCCGGGTGTA 
CTGGCTGGGA TTGTGCTGGG CGACTTGGTG CTGACTCTGC TCATCGCCCT GGCGGTGTAC 
TCTCTGGGCC GCCTGGTCTC TCGAGGCCGA GGGACTGCAG ACGGGACCCG GAAACAGCAC 
ATGGCTGAGA CTGAGTCACC TTATCAGGAG CTTCAGGGTC AGAGGCCAGA AGTATACAGT 
GACCTCAACA CACAGAGGCA GTATTACAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A DAP12-dependent signal promotes pro-inflammatory polarization in microglia following nerve injury and exacerbates degeneration of injured neurons.
Glia63(6)1073-82(2015 Jun)
Kobayashi M,Konishi H,Takai T,Kiyama H

DAP12 Stabilizes the C-terminal Fragment of the Triggering Receptor Expressed on Myeloid Cells-2 (TREM2) and Protects against LPS-induced Pro-inflammatory Response.
The Journal of biological chemistry290(25)15866-77(2015 Jun)
Zhong L,Chen XF,Zhang ZL,Wang Z,Shi XZ,Xu K,Zhang YW,Xu H,Bu G

DAP12 impacts trafficking and surface stability of killer immunoglobulin-like receptors on natural killer cells.
Journal of leukocyte biology94(2)301-13(2013 Aug)
Mulrooney TJ,Posch PE,Hurley CK

Siglec-15 protein regulates formation of functional osteoclasts in concert with DNAX-activating protein of 12 kDa (DAP12).
The Journal of biological chemistry287(21)17493-502(2012 May)
Ishida-Kitagawa N,Tanaka K,Bao X,Kimura T,Miura T,Kitaoka Y,Hayashi K,Sato M,Maruoka M,Ogawa T,Miyoshi J,Takeya T