Ogdh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ogdh
Entrez Gene ID	360975
Full Name	oxoglutarate dehydrogenase
General protein information	Preferred Names oxoglutarate dehydrogenase Names 2-oxoglutarate dehydrogenase, mitochondrial 2-oxoglutarate dehydrogenase E1 component, mitochondrial 2-oxoglutarate dehydrogenase complex component E1 OGDC-E1 alpha-ketoglutarate dehydrogenase oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) oxoglutarate dehydrogenase (lipoamide)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251448.3	XP_006251510.1	2-oxoglutarate dehydrogenase, mitochondrial isoform X2
XM_006251446.3	XP_006251508.1	2-oxoglutarate dehydrogenase, mitochondrial isoform X1
XM_006251447.2	XP_006251509.1	2-oxoglutarate dehydrogenase, mitochondrial isoform X1
NM_001017461.1	NP_001017461.1	2-oxoglutarate dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	OGDH	XP_005642239.1
Bos taurus (cattle)	OGDH	NP_001069498.1
Danio rerio (zebrafish)	ogdhb	XP_001338181.2
Danio rerio (zebrafish)	ogdha	NP_957073.2
Kluyveromyces lactis	KLLA0F04477g	XP_455282.1
Eremothecium gossypii	AGOS_AER374C	NP_985230.2
Arabidopsis thaliana (thale cress)	AT5G65750	NP_201376.1
Oryza sativa (rice)	Os04g0390000	NP_001052638.2
Homo sapiens (human)	OGDH	NP_002532.2
	OGDH	XP_001089063.1
Drosophila melanogaster (fruit fly)	Nc73EF	NP_788520.2
Saccharomyces cerevisiae (baker's yeast)	KGD1	NP_012141.1
Xenopus tropicalis (tropical clawed frog)	ogdh	NP_001135687.1
Rattus norvegicus (Norway rat)	Ogdh	NP_001017461.1
Neurospora crassa	NCU05425	XP_963248.2
Oryza sativa (rice)	Os07g0695800	NP_001060747.1
Pan troglodytes (chimpanzee)	OGDH	XP_001146811.2
Mus musculus (house mouse)	Ogdh	NP_001239211.1
Schizosaccharomyces pombe (fission yeast)	SPBC3H7.03c	NP_595772.1
Arabidopsis thaliana (thale cress)	AT3G55410	NP_191101.2
Gallus gallus (chicken)	OGDH	NP_001026553.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006366	XP_001688858.1
Caenorhabditis elegans (roundworm)	ogdh-1	NP_500617.1
Magnaporthe oryzae (rice blast fungus)	MGG_12767	XP_003716844.1

Gene Ontology
Bibliography

Related articles in PubMed

KAT2A coupled with the ?-KGDH complex acts as a histone H3 succinyltransferase.
Wang Y, Guo YR, Liu K, Yin Z, Liu R, Xia Y, Tan L, Yang P, Lee JH, Li XJ, Hawke D, Zheng Y, Qian X, Lyu J, He J, Xing D, Tao YJ, Lu Z
Nature552(7684)273-277(2017 Dec)

Thiamine Induces Long-Term Changes in Amino Acid Profiles and Activities of 2-Oxoglutarate and 2-Oxoadipate Dehydrogenases in Rat Brain.
Tsepkova PM, Artiukhov AV, Boyko AI, Aleshin VA, Mkrtchyan GV, Zvyagintseva MA, Ryabov SI, Ksenofontov AL, Baratova LA, Graf AV, Bunik VI
Biochemistry. Biokhimiia82(6)723-736(2017 Jun)

Calcium-insensitive splice variants of mammalian E1 subunit of 2-oxoglutarate dehydrogenase complex with tissue-specific patterns of expression.
Denton RM, Pullen TJ, Armstrong CT, Heesom KJ, Rutter GA
The Biochemical journal473(9)1165-78(2016 05)

Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium-binding site.
Armstrong CT, Anderson JL, Denton RM
The Biochemical journal459(2)369-81(2014 Apr)

The 2-oxoacid dehydrogenase complexes in mitochondria can produce superoxide/hydrogen peroxide at much higher rates than complex I.
Quinlan CL, Goncalves RL, Hey-Mogensen M, Yadava N, Bunik VI, Brand MD
The Journal of biological chemistry289(12)8312-25(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thiamine Induces Long-Term Changes in Amino Acid Profiles and Activities of 2-Oxoglutarate and 2-Oxoadipate Dehydrogenases in Rat Brain
Title: Thiamine Induces Long-Term Changes in Amino Acid Profiles and Activities of 2-Oxoglutarate and 2-Oxoadipate Dehydrogenases in Rat Brain.

Tissue-specific expression of OGDH splice variants may thus provide a mechanism that tunes the control of the enzyme to the specialized metabolic and signalling needs of individual cell types
Title: Calcium-insensitive splice variants of mammalian E1 subunit of 2-oxoglutarate dehydrogenase complex with tissue-specific patterns of expression.

H2O2 production from Ogdh can be considerable but may previously have been misattributed to complex I.
Title: The 2-oxoacid dehydrogenase complexes in mitochondria can produce superoxide/hydrogen peroxide at much higher rates than complex I.

Data support the assumption that neuroprotection by phosphonates involves their interaction with cellular OGDH.
Title: Phosphono analogues of 2-oxoglutarate protect cerebellar granule neurons upon glutamate excitotoxicity.

In contrast to the heart complex, where only the known OGDH was determined, the band corresponding to the brain OGDH component was found to also include the novel 2-oxoglutarate dehydrogenase-like (OGDHL) protein.
Title: Novel isoenzyme of 2-oxoglutarate dehydrogenase is identified in brain, but not in heart.

The following Ogdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ogdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12412	NM_001017461.1 Latest version!	Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa12412	XM_006251446.3 Latest version!	Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa12412	XM_006251447.2 Latest version!	Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa36377	XM_006251448.3 Latest version!	Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ogdh ( NM_001017461.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001017461.1 Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), mRNA.

Price & Availability↑

CloneID	ORa12412
Clone ID Related Accession (Same CDS sequence)	XM_006251446.3 , XM_006251447.2 , NM_001017461.1
Accession Version	NM_001017461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	2-oxoglutarate dehydrogenase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083811.1. On Apr 29, 2005 this sequence version replaced XM_573650.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083811.1, FQ222842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTTCATT TAAGGACTTG TGCTGCTAAG TTAAGGCCAT TGACAGCCTC CCAGACTGTT 
AAGACATTTT CACAAAACAA ACCAGCAGCA ATTAGGACGT TTCAACAGAT TCGGTGCTAT 
TCTGCACCTG TAGCTGCTGA ACCATTTCTT AGTGGGACTA GTTCGAACTA TGTGGAGGAA 
ATGTACTGTG CCTGGTTGGA GAATCCCAAA AGTGTACATA AGTCATGGGA CATTTTTTTC 
CGAAACACCA ATGCTGGAGC CCCACCGGGC ACTGCCTACC AGAGCCCCCT TTCCCTGAGT 
CGAAGCTCCC TGGCTACCAT GGCTCATGCA CAGTCCCTGG TGGAAGCACA GCCTAACGTC 
GACAAGCTCG TGGAGGACCA CTTGGCGGTG CAGTCTCTCA TCAGGGCGTA TCAGATACGA 
GGGCACCATG TAGCACAGCT GGACCCCCTG GGGATTTTGG ATGCTGATCT GGACTCCTCC 
GTGCCCGCTG ACATTATCTC ATCCACAGAC AAACTTGGGT TCTATGGCCT ACACGAGTCT 
GACCTTGACA AGGTCTTCCA CTTACCCACC ACCACTTTCA TCGGGGGACA GGAGCCAGCA 
CTTCCTCTTC GGGAGATCAT CCGTCGGCTG GAGATGGCCT ACTGCCAGCA CATTGGTGTG 
GAGTTCATGT TCATTAACGA TTTGGAGCAA TGCCAGTGGA TCCGGCAGAA GTTTGAGACC 
CCTGGGATCA TGCAGTTCAC CAATGAGGAG AAGCGGACCC TGCTGGCCAG GCTTGTACGG 
TCCACCAGGT TTGAGGAGTT CCTGCAGCGA AAGTGGTCCT CTGAGAAGCG TTTTGGTCTG 
GAAGGCTGTG AGGTGCTGAT CCCTGCCCTC AAGACAATCA TTGACATGTC AAGTGCAAAT 
GGAGTGGACT ATGTGATCAT GGGGATGCCA CACAGAGGAC GACTGAACGT GCTAGCCAAT 
GTCATCAGGA AGGAGCTGGA GCAGATATTC TGTCAGTTTG ACTCGAAGCT GGAGGCAGCT 
GATGAGGGTT CCGGGGACAT GAAGTACCAC CTGGGCATGT ATCACCGAAG GATCAACCGT 
GTGACCGACA GAAACATCAC TCTGTCCTTG GTGGCTAACC CTTCCCACTT AGAGGCTGCT 
GACCCTGTAG TGATGGGAAA GACCAAAGCT GAACAGTTCT ACTGTGGAGA CACTGAAGGG 
AAAAAGGTGA TGTCTATCCT GCTGCATGGG GATGCTGCCT TTGCTGGCCA GGGTATCGTG 
TATGAGACCT TCCATCTCAG CGACCTGCCA TCCTATACAA CCCACGGCAC CGTTCACGTG 
GTTGTCAACA ACCAGATTGG CTTCACCACA GACCCTCGGA TGGCCCGCTC TTCTCCCTAC 
CCCACTGATG TGGCCCGAGT GGTGAACGCC CCCATTTTCC ATGTCAACTC AGATGACCCT 
GAAGCTGTCA TGTATGTATG CAAGGTGGCA GCTGAGTGGA GAAACACCTT CCACAAGGAT 
GTTGTTGTTG ATCTGGTGTG TTATCGACGA AATGGCCACA ACGAGATGGA TGAACCTATG 
TTCACACAGC CACTCATGTA CAAGCAGATC CGTAAGCAGA AGCCGGTACT GCAGAAGTAT 
GCAGAATTGC TGGTATCCCA GGGGGTCGTC AACCAGCCCG AATATGAGGA GGAAATCTCC 
AAGTACGATA AGATCTGTGA GGAAGCATTT ACCAGATCCA AAGATGAGAA GATCTTGCAC 
ATCAAGCACT GGCTGGATTC CCCCTGGCCT GGCTTTTTCA CCCTGGATGG ACAGCCCAGG 
AGCATGACCT GCCCCTCCAC TGGCCTGGAG GAGGACATCT TGACCCACAT TGGGAATGTG 
GCCAGTTCTG TACCTGTGGA GAACTTTACC ATCCATGGAG GGCTGAGCCG GATCTTGAAG 
ACTCGCAGAG AGCTTGTGAC AAACCGGACT GTGGACTGGG CCCTGGCAGA GTACATGGCG 
TTCGGATCAC TCCTGAAGGA AGGCATCCAT GTCCGGCTGA GTGGCCAGGA CGTGGAGCGG 
GGTACCTTCA GCCATCGCCA CCATGTGCTC CATGACCAGA ATGTGGACAA AAGAACCTGC 
ATCCCCATGA ACCACCTTTG GCCTAACCAG GCCCCTTACA CCGTGTGCAA CAGCTCGTTG 
TCTGAGTATG GTGTCCTGGG CTTTGAACTG GGCTTTGCCA TGGCTAGCCC TAATGCTCTG 
GTTCTCTGGG AGGCCCAGTT TGGTGATTTC AACAACATGG CACAGTGCAT CATTGACCAG 
TTCATCTGCC CAGGACAGGC AAAGTGGGTG CGGCAGAATG GCATCGTGCT CCTGCTACCT 
CACGGCATGG AAGGCATGGG TCCCGAGCAT TCCTCTGCCC GTCCAGAGAG GTTTCTGCAG 
ATGTGCAATG ATGACCCAGA TGTCCTGCCT AACCTGCAGG AGGAAAACTT TGACATCAGT 
CAGCTCTACG ACTGCAACTG GATTGTCGTC AACTGCTCCA CCCCTGGCAA CTTCTTCCAC 
GTGCTGCGAC GACAGATCTT GCTGCCCTTC CGGAAGCCGT TAATCGTCTT CACTCCCAAA 
TCCCTCCTGC GCCACCCTGA GGCAAGAACT AGCTTTGATG AGATGCTGCC AGGAACCCAC 
TTCCAGCGTG TGATCCCAGA AGATGGACCT GCAGCTCAGA ACCCAGACAA AGTCAAGAGG 
CTTCTCTTCT GCACTGGCAA GGTGTACTAT GACCTCACCC GAGAGCGCAA AGCCAGGGAC 
ATGGCAGAGG AGGTGGCTAT TACAAGGATT GAGCAGCTGT CACCATTCCC CTTCGACCTC 
CTGCTGAAAG AGGCACAGAA GTATCCCAAT GCTGAGCTGG CCTGGTGCCA GGAAGAGCAC 
AAGAATCAAG GCTACTATGA CTATGTCAAG CCAAGACTTC GGACCACCAT TGACCGTGCT 
AAGCCTGTCT GGTATGCTGG CCGAGACCCA GCAGCCGCTC CAGCCACTGG CAACAAGAAG 
ACCCACCTGA CAGAGCTGCA GCGCTTTCTG GACACAGCCT TTGACCTGGA CGCCTTCAAG 
AAATTCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017461.1
CDS	79..3150
Translation	MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRSSLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDMKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKICEEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDILTHIGNVASSVPVENFTIHGGLSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPNLQEENFDISQLYDCNWIVVNCSTPGNFFHVLRRQILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPEDGPAAQNPDKVKRLLFCTGKVYYDLTRERKARDMAEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRAKPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS

Target ORF information:

RefSeq Version	NM_001017461.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017461.1

ORF Insert Sequence:

ATGTTTCATT TAAGGACTTG TGCTGCTAAG TTAAGGCCAT TGACAGCCTC CCAGACTGTT 
AAGACATTTT CACAAAACAA ACCAGCAGCA ATTAGGACGT TTCAACAGAT TCGGTGCTAT 
TCTGCACCTG TAGCTGCTGA ACCATTTCTT AGTGGGACTA GTTCGAACTA TGTGGAGGAA 
ATGTACTGTG CCTGGTTGGA GAATCCCAAA AGTGTACATA AGTCATGGGA CATTTTTTTC 
CGAAACACCA ATGCTGGAGC CCCACCGGGC ACTGCCTACC AGAGCCCCCT TTCCCTGAGT 
CGAAGCTCCC TGGCTACCAT GGCTCATGCA CAGTCCCTGG TGGAAGCACA GCCTAACGTC 
GACAAGCTCG TGGAGGACCA CTTGGCGGTG CAGTCTCTCA TCAGGGCGTA TCAGATACGA 
GGGCACCATG TAGCACAGCT GGACCCCCTG GGGATTTTGG ATGCTGATCT GGACTCCTCC 
GTGCCCGCTG ACATTATCTC ATCCACAGAC AAACTTGGGT TCTATGGCCT ACACGAGTCT 
GACCTTGACA AGGTCTTCCA CTTACCCACC ACCACTTTCA TCGGGGGACA GGAGCCAGCA 
CTTCCTCTTC GGGAGATCAT CCGTCGGCTG GAGATGGCCT ACTGCCAGCA CATTGGTGTG 
GAGTTCATGT TCATTAACGA TTTGGAGCAA TGCCAGTGGA TCCGGCAGAA GTTTGAGACC 
CCTGGGATCA TGCAGTTCAC CAATGAGGAG AAGCGGACCC TGCTGGCCAG GCTTGTACGG 
TCCACCAGGT TTGAGGAGTT CCTGCAGCGA AAGTGGTCCT CTGAGAAGCG TTTTGGTCTG 
GAAGGCTGTG AGGTGCTGAT CCCTGCCCTC AAGACAATCA TTGACATGTC AAGTGCAAAT 
GGAGTGGACT ATGTGATCAT GGGGATGCCA CACAGAGGAC GACTGAACGT GCTAGCCAAT 
GTCATCAGGA AGGAGCTGGA GCAGATATTC TGTCAGTTTG ACTCGAAGCT GGAGGCAGCT 
GATGAGGGTT CCGGGGACAT GAAGTACCAC CTGGGCATGT ATCACCGAAG GATCAACCGT 
GTGACCGACA GAAACATCAC TCTGTCCTTG GTGGCTAACC CTTCCCACTT AGAGGCTGCT 
GACCCTGTAG TGATGGGAAA GACCAAAGCT GAACAGTTCT ACTGTGGAGA CACTGAAGGG 
AAAAAGGTGA TGTCTATCCT GCTGCATGGG GATGCTGCCT TTGCTGGCCA GGGTATCGTG 
TATGAGACCT TCCATCTCAG CGACCTGCCA TCCTATACAA CCCACGGCAC CGTTCACGTG 
GTTGTCAACA ACCAGATTGG CTTCACCACA GACCCTCGGA TGGCCCGCTC TTCTCCCTAC 
CCCACTGATG TGGCCCGAGT GGTGAACGCC CCCATTTTCC ATGTCAACTC AGATGACCCT 
GAAGCTGTCA TGTATGTATG CAAGGTGGCA GCTGAGTGGA GAAACACCTT CCACAAGGAT 
GTTGTTGTTG ATCTGGTGTG TTATCGACGA AATGGCCACA ACGAGATGGA TGAACCTATG 
TTCACACAGC CACTCATGTA CAAGCAGATC CGTAAGCAGA AGCCGGTACT GCAGAAGTAT 
GCAGAATTGC TGGTATCCCA GGGGGTCGTC AACCAGCCCG AATATGAGGA GGAAATCTCC 
AAGTACGATA AGATCTGTGA GGAAGCATTT ACCAGATCCA AAGATGAGAA GATCTTGCAC 
ATCAAGCACT GGCTGGATTC CCCCTGGCCT GGCTTTTTCA CCCTGGATGG ACAGCCCAGG 
AGCATGACCT GCCCCTCCAC TGGCCTGGAG GAGGACATCT TGACCCACAT TGGGAATGTG 
GCCAGTTCTG TACCTGTGGA GAACTTTACC ATCCATGGAG GGCTGAGCCG GATCTTGAAG 
ACTCGCAGAG AGCTTGTGAC AAACCGGACT GTGGACTGGG CCCTGGCAGA GTACATGGCG 
TTCGGATCAC TCCTGAAGGA AGGCATCCAT GTCCGGCTGA GTGGCCAGGA CGTGGAGCGG 
GGTACCTTCA GCCATCGCCA CCATGTGCTC CATGACCAGA ATGTGGACAA AAGAACCTGC 
ATCCCCATGA ACCACCTTTG GCCTAACCAG GCCCCTTACA CCGTGTGCAA CAGCTCGTTG 
TCTGAGTATG GTGTCCTGGG CTTTGAACTG GGCTTTGCCA TGGCTAGCCC TAATGCTCTG 
GTTCTCTGGG AGGCCCAGTT TGGTGATTTC AACAACATGG CACAGTGCAT CATTGACCAG 
TTCATCTGCC CAGGACAGGC AAAGTGGGTG CGGCAGAATG GCATCGTGCT CCTGCTACCT 
CACGGCATGG AAGGCATGGG TCCCGAGCAT TCCTCTGCCC GTCCAGAGAG GTTTCTGCAG 
ATGTGCAATG ATGACCCAGA TGTCCTGCCT AACCTGCAGG AGGAAAACTT TGACATCAGT 
CAGCTCTACG ACTGCAACTG GATTGTCGTC AACTGCTCCA CCCCTGGCAA CTTCTTCCAC 
GTGCTGCGAC GACAGATCTT GCTGCCCTTC CGGAAGCCGT TAATCGTCTT CACTCCCAAA 
TCCCTCCTGC GCCACCCTGA GGCAAGAACT AGCTTTGATG AGATGCTGCC AGGAACCCAC 
TTCCAGCGTG TGATCCCAGA AGATGGACCT GCAGCTCAGA ACCCAGACAA AGTCAAGAGG 
CTTCTCTTCT GCACTGGCAA GGTGTACTAT GACCTCACCC GAGAGCGCAA AGCCAGGGAC 
ATGGCAGAGG AGGTGGCTAT TACAAGGATT GAGCAGCTGT CACCATTCCC CTTCGACCTC 
CTGCTGAAAG AGGCACAGAA GTATCCCAAT GCTGAGCTGG CCTGGTGCCA GGAAGAGCAC 
AAGAATCAAG GCTACTATGA CTATGTCAAG CCAAGACTTC GGACCACCAT TGACCGTGCT 
AAGCCTGTCT GGTATGCTGG CCGAGACCCA GCAGCCGCTC CAGCCACTGG CAACAAGAAG 
ACCCACCTGA CAGAGCTGCA GCGCTTTCTG GACACAGCCT TTGACCTGGA CGCCTTCAAG 
AAATTCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ogdh ( XM_006251446.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251508.1 Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12412
Clone ID Related Accession (Same CDS sequence)	XM_006251446.3 , XM_006251447.2 , NM_001017461.1
Accession Version	XM_006251446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	2-oxoglutarate dehydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCATT TAAGGACTTG TGCTGCTAAG TTAAGGCCAT TGACAGCCTC CCAGACTGTT 
AAGACATTTT CACAAAACAA ACCAGCAGCA ATTAGGACGT TTCAACAGAT TCGGTGCTAT 
TCTGCACCTG TAGCTGCTGA ACCATTTCTT AGTGGGACTA GTTCGAACTA TGTGGAGGAA 
ATGTACTGTG CCTGGTTGGA GAATCCCAAA AGTGTACATA AGTCATGGGA CATTTTTTTC 
CGAAACACCA ATGCTGGAGC CCCACCGGGC ACTGCCTACC AGAGCCCCCT TTCCCTGAGT 
CGAAGCTCCC TGGCTACCAT GGCTCATGCA CAGTCCCTGG TGGAAGCACA GCCTAACGTC 
GACAAGCTCG TGGAGGACCA CTTGGCGGTG CAGTCTCTCA TCAGGGCGTA TCAGATACGA 
GGGCACCATG TAGCACAGCT GGACCCCCTG GGGATTTTGG ATGCTGATCT GGACTCCTCC 
GTGCCCGCTG ACATTATCTC ATCCACAGAC AAACTTGGGT TCTATGGCCT ACACGAGTCT 
GACCTTGACA AGGTCTTCCA CTTACCCACC ACCACTTTCA TCGGGGGACA GGAGCCAGCA 
CTTCCTCTTC GGGAGATCAT CCGTCGGCTG GAGATGGCCT ACTGCCAGCA CATTGGTGTG 
GAGTTCATGT TCATTAACGA TTTGGAGCAA TGCCAGTGGA TCCGGCAGAA GTTTGAGACC 
CCTGGGATCA TGCAGTTCAC CAATGAGGAG AAGCGGACCC TGCTGGCCAG GCTTGTACGG 
TCCACCAGGT TTGAGGAGTT CCTGCAGCGA AAGTGGTCCT CTGAGAAGCG TTTTGGTCTG 
GAAGGCTGTG AGGTGCTGAT CCCTGCCCTC AAGACAATCA TTGACATGTC AAGTGCAAAT 
GGAGTGGACT ATGTGATCAT GGGGATGCCA CACAGAGGAC GACTGAACGT GCTAGCCAAT 
GTCATCAGGA AGGAGCTGGA GCAGATATTC TGTCAGTTTG ACTCGAAGCT GGAGGCAGCT 
GATGAGGGTT CCGGGGACAT GAAGTACCAC CTGGGCATGT ATCACCGAAG GATCAACCGT 
GTGACCGACA GAAACATCAC TCTGTCCTTG GTGGCTAACC CTTCCCACTT AGAGGCTGCT 
GACCCTGTAG TGATGGGAAA GACCAAAGCT GAACAGTTCT ACTGTGGAGA CACTGAAGGG 
AAAAAGGTGA TGTCTATCCT GCTGCATGGG GATGCTGCCT TTGCTGGCCA GGGTATCGTG 
TATGAGACCT TCCATCTCAG CGACCTGCCA TCCTATACAA CCCACGGCAC CGTTCACGTG 
GTTGTCAACA ACCAGATTGG CTTCACCACA GACCCTCGGA TGGCCCGCTC TTCTCCCTAC 
CCCACTGATG TGGCCCGAGT GGTGAACGCC CCCATTTTCC ATGTCAACTC AGATGACCCT 
GAAGCTGTCA TGTATGTATG CAAGGTGGCA GCTGAGTGGA GAAACACCTT CCACAAGGAT 
GTTGTTGTTG ATCTGGTGTG TTATCGACGA AATGGCCACA ACGAGATGGA TGAACCTATG 
TTCACACAGC CACTCATGTA CAAGCAGATC CGTAAGCAGA AGCCGGTACT GCAGAAGTAT 
GCAGAATTGC TGGTATCCCA GGGGGTCGTC AACCAGCCCG AATATGAGGA GGAAATCTCC 
AAGTACGATA AGATCTGTGA GGAAGCATTT ACCAGATCCA AAGATGAGAA GATCTTGCAC 
ATCAAGCACT GGCTGGATTC CCCCTGGCCT GGCTTTTTCA CCCTGGATGG ACAGCCCAGG 
AGCATGACCT GCCCCTCCAC TGGCCTGGAG GAGGACATCT TGACCCACAT TGGGAATGTG 
GCCAGTTCTG TACCTGTGGA GAACTTTACC ATCCATGGAG GGCTGAGCCG GATCTTGAAG 
ACTCGCAGAG AGCTTGTGAC AAACCGGACT GTGGACTGGG CCCTGGCAGA GTACATGGCG 
TTCGGATCAC TCCTGAAGGA AGGCATCCAT GTCCGGCTGA GTGGCCAGGA CGTGGAGCGG 
GGTACCTTCA GCCATCGCCA CCATGTGCTC CATGACCAGA ATGTGGACAA AAGAACCTGC 
ATCCCCATGA ACCACCTTTG GCCTAACCAG GCCCCTTACA CCGTGTGCAA CAGCTCGTTG 
TCTGAGTATG GTGTCCTGGG CTTTGAACTG GGCTTTGCCA TGGCTAGCCC TAATGCTCTG 
GTTCTCTGGG AGGCCCAGTT TGGTGATTTC AACAACATGG CACAGTGCAT CATTGACCAG 
TTCATCTGCC CAGGACAGGC AAAGTGGGTG CGGCAGAATG GCATCGTGCT CCTGCTACCT 
CACGGCATGG AAGGCATGGG TCCCGAGCAT TCCTCTGCCC GTCCAGAGAG GTTTCTGCAG 
ATGTGCAATG ATGACCCAGA TGTCCTGCCT AACCTGCAGG AGGAAAACTT TGACATCAGT 
CAGCTCTACG ACTGCAACTG GATTGTCGTC AACTGCTCCA CCCCTGGCAA CTTCTTCCAC 
GTGCTGCGAC GACAGATCTT GCTGCCCTTC CGGAAGCCGT TAATCGTCTT CACTCCCAAA 
TCCCTCCTGC GCCACCCTGA GGCAAGAACT AGCTTTGATG AGATGCTGCC AGGAACCCAC 
TTCCAGCGTG TGATCCCAGA AGATGGACCT GCAGCTCAGA ACCCAGACAA AGTCAAGAGG 
CTTCTCTTCT GCACTGGCAA GGTGTACTAT GACCTCACCC GAGAGCGCAA AGCCAGGGAC 
ATGGCAGAGG AGGTGGCTAT TACAAGGATT GAGCAGCTGT CACCATTCCC CTTCGACCTC 
CTGCTGAAAG AGGCACAGAA GTATCCCAAT GCTGAGCTGG CCTGGTGCCA GGAAGAGCAC 
AAGAATCAAG GCTACTATGA CTATGTCAAG CCAAGACTTC GGACCACCAT TGACCGTGCT 
AAGCCTGTCT GGTATGCTGG CCGAGACCCA GCAGCCGCTC CAGCCACTGG CAACAAGAAG 
ACCCACCTGA CAGAGCTGCA GCGCTTTCTG GACACAGCCT TTGACCTGGA CGCCTTCAAG 
AAATTCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251508.1
CDS	194..3265
Translation	MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRSSLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDMKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKICEEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDILTHIGNVASSVPVENFTIHGGLSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPNLQEENFDISQLYDCNWIVVNCSTPGNFFHVLRRQILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPEDGPAAQNPDKVKRLLFCTGKVYYDLTRERKARDMAEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRAKPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS

Target ORF information:

RefSeq Version	XM_006251446.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251446.3

ORF Insert Sequence:

ATGTTTCATT TAAGGACTTG TGCTGCTAAG TTAAGGCCAT TGACAGCCTC CCAGACTGTT 
AAGACATTTT CACAAAACAA ACCAGCAGCA ATTAGGACGT TTCAACAGAT TCGGTGCTAT 
TCTGCACCTG TAGCTGCTGA ACCATTTCTT AGTGGGACTA GTTCGAACTA TGTGGAGGAA 
ATGTACTGTG CCTGGTTGGA GAATCCCAAA AGTGTACATA AGTCATGGGA CATTTTTTTC 
CGAAACACCA ATGCTGGAGC CCCACCGGGC ACTGCCTACC AGAGCCCCCT TTCCCTGAGT 
CGAAGCTCCC TGGCTACCAT GGCTCATGCA CAGTCCCTGG TGGAAGCACA GCCTAACGTC 
GACAAGCTCG TGGAGGACCA CTTGGCGGTG CAGTCTCTCA TCAGGGCGTA TCAGATACGA 
GGGCACCATG TAGCACAGCT GGACCCCCTG GGGATTTTGG ATGCTGATCT GGACTCCTCC 
GTGCCCGCTG ACATTATCTC ATCCACAGAC AAACTTGGGT TCTATGGCCT ACACGAGTCT 
GACCTTGACA AGGTCTTCCA CTTACCCACC ACCACTTTCA TCGGGGGACA GGAGCCAGCA 
CTTCCTCTTC GGGAGATCAT CCGTCGGCTG GAGATGGCCT ACTGCCAGCA CATTGGTGTG 
GAGTTCATGT TCATTAACGA TTTGGAGCAA TGCCAGTGGA TCCGGCAGAA GTTTGAGACC 
CCTGGGATCA TGCAGTTCAC CAATGAGGAG AAGCGGACCC TGCTGGCCAG GCTTGTACGG 
TCCACCAGGT TTGAGGAGTT CCTGCAGCGA AAGTGGTCCT CTGAGAAGCG TTTTGGTCTG 
GAAGGCTGTG AGGTGCTGAT CCCTGCCCTC AAGACAATCA TTGACATGTC AAGTGCAAAT 
GGAGTGGACT ATGTGATCAT GGGGATGCCA CACAGAGGAC GACTGAACGT GCTAGCCAAT 
GTCATCAGGA AGGAGCTGGA GCAGATATTC TGTCAGTTTG ACTCGAAGCT GGAGGCAGCT 
GATGAGGGTT CCGGGGACAT GAAGTACCAC CTGGGCATGT ATCACCGAAG GATCAACCGT 
GTGACCGACA GAAACATCAC TCTGTCCTTG GTGGCTAACC CTTCCCACTT AGAGGCTGCT 
GACCCTGTAG TGATGGGAAA GACCAAAGCT GAACAGTTCT ACTGTGGAGA CACTGAAGGG 
AAAAAGGTGA TGTCTATCCT GCTGCATGGG GATGCTGCCT TTGCTGGCCA GGGTATCGTG 
TATGAGACCT TCCATCTCAG CGACCTGCCA TCCTATACAA CCCACGGCAC CGTTCACGTG 
GTTGTCAACA ACCAGATTGG CTTCACCACA GACCCTCGGA TGGCCCGCTC TTCTCCCTAC 
CCCACTGATG TGGCCCGAGT GGTGAACGCC CCCATTTTCC ATGTCAACTC AGATGACCCT 
GAAGCTGTCA TGTATGTATG CAAGGTGGCA GCTGAGTGGA GAAACACCTT CCACAAGGAT 
GTTGTTGTTG ATCTGGTGTG TTATCGACGA AATGGCCACA ACGAGATGGA TGAACCTATG 
TTCACACAGC CACTCATGTA CAAGCAGATC CGTAAGCAGA AGCCGGTACT GCAGAAGTAT 
GCAGAATTGC TGGTATCCCA GGGGGTCGTC AACCAGCCCG AATATGAGGA GGAAATCTCC 
AAGTACGATA AGATCTGTGA GGAAGCATTT ACCAGATCCA AAGATGAGAA GATCTTGCAC 
ATCAAGCACT GGCTGGATTC CCCCTGGCCT GGCTTTTTCA CCCTGGATGG ACAGCCCAGG 
AGCATGACCT GCCCCTCCAC TGGCCTGGAG GAGGACATCT TGACCCACAT TGGGAATGTG 
GCCAGTTCTG TACCTGTGGA GAACTTTACC ATCCATGGAG GGCTGAGCCG GATCTTGAAG 
ACTCGCAGAG AGCTTGTGAC AAACCGGACT GTGGACTGGG CCCTGGCAGA GTACATGGCG 
TTCGGATCAC TCCTGAAGGA AGGCATCCAT GTCCGGCTGA GTGGCCAGGA CGTGGAGCGG 
GGTACCTTCA GCCATCGCCA CCATGTGCTC CATGACCAGA ATGTGGACAA AAGAACCTGC 
ATCCCCATGA ACCACCTTTG GCCTAACCAG GCCCCTTACA CCGTGTGCAA CAGCTCGTTG 
TCTGAGTATG GTGTCCTGGG CTTTGAACTG GGCTTTGCCA TGGCTAGCCC TAATGCTCTG 
GTTCTCTGGG AGGCCCAGTT TGGTGATTTC AACAACATGG CACAGTGCAT CATTGACCAG 
TTCATCTGCC CAGGACAGGC AAAGTGGGTG CGGCAGAATG GCATCGTGCT CCTGCTACCT 
CACGGCATGG AAGGCATGGG TCCCGAGCAT TCCTCTGCCC GTCCAGAGAG GTTTCTGCAG 
ATGTGCAATG ATGACCCAGA TGTCCTGCCT AACCTGCAGG AGGAAAACTT TGACATCAGT 
CAGCTCTACG ACTGCAACTG GATTGTCGTC AACTGCTCCA CCCCTGGCAA CTTCTTCCAC 
GTGCTGCGAC GACAGATCTT GCTGCCCTTC CGGAAGCCGT TAATCGTCTT CACTCCCAAA 
TCCCTCCTGC GCCACCCTGA GGCAAGAACT AGCTTTGATG AGATGCTGCC AGGAACCCAC 
TTCCAGCGTG TGATCCCAGA AGATGGACCT GCAGCTCAGA ACCCAGACAA AGTCAAGAGG 
CTTCTCTTCT GCACTGGCAA GGTGTACTAT GACCTCACCC GAGAGCGCAA AGCCAGGGAC 
ATGGCAGAGG AGGTGGCTAT TACAAGGATT GAGCAGCTGT CACCATTCCC CTTCGACCTC 
CTGCTGAAAG AGGCACAGAA GTATCCCAAT GCTGAGCTGG CCTGGTGCCA GGAAGAGCAC 
AAGAATCAAG GCTACTATGA CTATGTCAAG CCAAGACTTC GGACCACCAT TGACCGTGCT 
AAGCCTGTCT GGTATGCTGG CCGAGACCCA GCAGCCGCTC CAGCCACTGG CAACAAGAAG 
ACCCACCTGA CAGAGCTGCA GCGCTTTCTG GACACAGCCT TTGACCTGGA CGCCTTCAAG 
AAATTCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ogdh ( XM_006251447.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251509.1 Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12412
Clone ID Related Accession (Same CDS sequence)	XM_006251446.3 , XM_006251447.2 , NM_001017461.1
Accession Version	XM_006251447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	2-oxoglutarate dehydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006251447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCATT TAAGGACTTG TGCTGCTAAG TTAAGGCCAT TGACAGCCTC CCAGACTGTT 
AAGACATTTT CACAAAACAA ACCAGCAGCA ATTAGGACGT TTCAACAGAT TCGGTGCTAT 
TCTGCACCTG TAGCTGCTGA ACCATTTCTT AGTGGGACTA GTTCGAACTA TGTGGAGGAA 
ATGTACTGTG CCTGGTTGGA GAATCCCAAA AGTGTACATA AGTCATGGGA CATTTTTTTC 
CGAAACACCA ATGCTGGAGC CCCACCGGGC ACTGCCTACC AGAGCCCCCT TTCCCTGAGT 
CGAAGCTCCC TGGCTACCAT GGCTCATGCA CAGTCCCTGG TGGAAGCACA GCCTAACGTC 
GACAAGCTCG TGGAGGACCA CTTGGCGGTG CAGTCTCTCA TCAGGGCGTA TCAGATACGA 
GGGCACCATG TAGCACAGCT GGACCCCCTG GGGATTTTGG ATGCTGATCT GGACTCCTCC 
GTGCCCGCTG ACATTATCTC ATCCACAGAC AAACTTGGGT TCTATGGCCT ACACGAGTCT 
GACCTTGACA AGGTCTTCCA CTTACCCACC ACCACTTTCA TCGGGGGACA GGAGCCAGCA 
CTTCCTCTTC GGGAGATCAT CCGTCGGCTG GAGATGGCCT ACTGCCAGCA CATTGGTGTG 
GAGTTCATGT TCATTAACGA TTTGGAGCAA TGCCAGTGGA TCCGGCAGAA GTTTGAGACC 
CCTGGGATCA TGCAGTTCAC CAATGAGGAG AAGCGGACCC TGCTGGCCAG GCTTGTACGG 
TCCACCAGGT TTGAGGAGTT CCTGCAGCGA AAGTGGTCCT CTGAGAAGCG TTTTGGTCTG 
GAAGGCTGTG AGGTGCTGAT CCCTGCCCTC AAGACAATCA TTGACATGTC AAGTGCAAAT 
GGAGTGGACT ATGTGATCAT GGGGATGCCA CACAGAGGAC GACTGAACGT GCTAGCCAAT 
GTCATCAGGA AGGAGCTGGA GCAGATATTC TGTCAGTTTG ACTCGAAGCT GGAGGCAGCT 
GATGAGGGTT CCGGGGACAT GAAGTACCAC CTGGGCATGT ATCACCGAAG GATCAACCGT 
GTGACCGACA GAAACATCAC TCTGTCCTTG GTGGCTAACC CTTCCCACTT AGAGGCTGCT 
GACCCTGTAG TGATGGGAAA GACCAAAGCT GAACAGTTCT ACTGTGGAGA CACTGAAGGG 
AAAAAGGTGA TGTCTATCCT GCTGCATGGG GATGCTGCCT TTGCTGGCCA GGGTATCGTG 
TATGAGACCT TCCATCTCAG CGACCTGCCA TCCTATACAA CCCACGGCAC CGTTCACGTG 
GTTGTCAACA ACCAGATTGG CTTCACCACA GACCCTCGGA TGGCCCGCTC TTCTCCCTAC 
CCCACTGATG TGGCCCGAGT GGTGAACGCC CCCATTTTCC ATGTCAACTC AGATGACCCT 
GAAGCTGTCA TGTATGTATG CAAGGTGGCA GCTGAGTGGA GAAACACCTT CCACAAGGAT 
GTTGTTGTTG ATCTGGTGTG TTATCGACGA AATGGCCACA ACGAGATGGA TGAACCTATG 
TTCACACAGC CACTCATGTA CAAGCAGATC CGTAAGCAGA AGCCGGTACT GCAGAAGTAT 
GCAGAATTGC TGGTATCCCA GGGGGTCGTC AACCAGCCCG AATATGAGGA GGAAATCTCC 
AAGTACGATA AGATCTGTGA GGAAGCATTT ACCAGATCCA AAGATGAGAA GATCTTGCAC 
ATCAAGCACT GGCTGGATTC CCCCTGGCCT GGCTTTTTCA CCCTGGATGG ACAGCCCAGG 
AGCATGACCT GCCCCTCCAC TGGCCTGGAG GAGGACATCT TGACCCACAT TGGGAATGTG 
GCCAGTTCTG TACCTGTGGA GAACTTTACC ATCCATGGAG GGCTGAGCCG GATCTTGAAG 
ACTCGCAGAG AGCTTGTGAC AAACCGGACT GTGGACTGGG CCCTGGCAGA GTACATGGCG 
TTCGGATCAC TCCTGAAGGA AGGCATCCAT GTCCGGCTGA GTGGCCAGGA CGTGGAGCGG 
GGTACCTTCA GCCATCGCCA CCATGTGCTC CATGACCAGA ATGTGGACAA AAGAACCTGC 
ATCCCCATGA ACCACCTTTG GCCTAACCAG GCCCCTTACA CCGTGTGCAA CAGCTCGTTG 
TCTGAGTATG GTGTCCTGGG CTTTGAACTG GGCTTTGCCA TGGCTAGCCC TAATGCTCTG 
GTTCTCTGGG AGGCCCAGTT TGGTGATTTC AACAACATGG CACAGTGCAT CATTGACCAG 
TTCATCTGCC CAGGACAGGC AAAGTGGGTG CGGCAGAATG GCATCGTGCT CCTGCTACCT 
CACGGCATGG AAGGCATGGG TCCCGAGCAT TCCTCTGCCC GTCCAGAGAG GTTTCTGCAG 
ATGTGCAATG ATGACCCAGA TGTCCTGCCT AACCTGCAGG AGGAAAACTT TGACATCAGT 
CAGCTCTACG ACTGCAACTG GATTGTCGTC AACTGCTCCA CCCCTGGCAA CTTCTTCCAC 
GTGCTGCGAC GACAGATCTT GCTGCCCTTC CGGAAGCCGT TAATCGTCTT CACTCCCAAA 
TCCCTCCTGC GCCACCCTGA GGCAAGAACT AGCTTTGATG AGATGCTGCC AGGAACCCAC 
TTCCAGCGTG TGATCCCAGA AGATGGACCT GCAGCTCAGA ACCCAGACAA AGTCAAGAGG 
CTTCTCTTCT GCACTGGCAA GGTGTACTAT GACCTCACCC GAGAGCGCAA AGCCAGGGAC 
ATGGCAGAGG AGGTGGCTAT TACAAGGATT GAGCAGCTGT CACCATTCCC CTTCGACCTC 
CTGCTGAAAG AGGCACAGAA GTATCCCAAT GCTGAGCTGG CCTGGTGCCA GGAAGAGCAC 
AAGAATCAAG GCTACTATGA CTATGTCAAG CCAAGACTTC GGACCACCAT TGACCGTGCT 
AAGCCTGTCT GGTATGCTGG CCGAGACCCA GCAGCCGCTC CAGCCACTGG CAACAAGAAG 
ACCCACCTGA CAGAGCTGCA GCGCTTTCTG GACACAGCCT TTGACCTGGA CGCCTTCAAG 
AAATTCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251509.1
CDS	341..3412
Translation	MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRSSLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDMKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKICEEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDILTHIGNVASSVPVENFTIHGGLSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPNLQEENFDISQLYDCNWIVVNCSTPGNFFHVLRRQILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPEDGPAAQNPDKVKRLLFCTGKVYYDLTRERKARDMAEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRAKPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS

Target ORF information:

RefSeq Version	XM_006251447.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251447.2

ORF Insert Sequence:

ATGTTTCATT TAAGGACTTG TGCTGCTAAG TTAAGGCCAT TGACAGCCTC CCAGACTGTT 
AAGACATTTT CACAAAACAA ACCAGCAGCA ATTAGGACGT TTCAACAGAT TCGGTGCTAT 
TCTGCACCTG TAGCTGCTGA ACCATTTCTT AGTGGGACTA GTTCGAACTA TGTGGAGGAA 
ATGTACTGTG CCTGGTTGGA GAATCCCAAA AGTGTACATA AGTCATGGGA CATTTTTTTC 
CGAAACACCA ATGCTGGAGC CCCACCGGGC ACTGCCTACC AGAGCCCCCT TTCCCTGAGT 
CGAAGCTCCC TGGCTACCAT GGCTCATGCA CAGTCCCTGG TGGAAGCACA GCCTAACGTC 
GACAAGCTCG TGGAGGACCA CTTGGCGGTG CAGTCTCTCA TCAGGGCGTA TCAGATACGA 
GGGCACCATG TAGCACAGCT GGACCCCCTG GGGATTTTGG ATGCTGATCT GGACTCCTCC 
GTGCCCGCTG ACATTATCTC ATCCACAGAC AAACTTGGGT TCTATGGCCT ACACGAGTCT 
GACCTTGACA AGGTCTTCCA CTTACCCACC ACCACTTTCA TCGGGGGACA GGAGCCAGCA 
CTTCCTCTTC GGGAGATCAT CCGTCGGCTG GAGATGGCCT ACTGCCAGCA CATTGGTGTG 
GAGTTCATGT TCATTAACGA TTTGGAGCAA TGCCAGTGGA TCCGGCAGAA GTTTGAGACC 
CCTGGGATCA TGCAGTTCAC CAATGAGGAG AAGCGGACCC TGCTGGCCAG GCTTGTACGG 
TCCACCAGGT TTGAGGAGTT CCTGCAGCGA AAGTGGTCCT CTGAGAAGCG TTTTGGTCTG 
GAAGGCTGTG AGGTGCTGAT CCCTGCCCTC AAGACAATCA TTGACATGTC AAGTGCAAAT 
GGAGTGGACT ATGTGATCAT GGGGATGCCA CACAGAGGAC GACTGAACGT GCTAGCCAAT 
GTCATCAGGA AGGAGCTGGA GCAGATATTC TGTCAGTTTG ACTCGAAGCT GGAGGCAGCT 
GATGAGGGTT CCGGGGACAT GAAGTACCAC CTGGGCATGT ATCACCGAAG GATCAACCGT 
GTGACCGACA GAAACATCAC TCTGTCCTTG GTGGCTAACC CTTCCCACTT AGAGGCTGCT 
GACCCTGTAG TGATGGGAAA GACCAAAGCT GAACAGTTCT ACTGTGGAGA CACTGAAGGG 
AAAAAGGTGA TGTCTATCCT GCTGCATGGG GATGCTGCCT TTGCTGGCCA GGGTATCGTG 
TATGAGACCT TCCATCTCAG CGACCTGCCA TCCTATACAA CCCACGGCAC CGTTCACGTG 
GTTGTCAACA ACCAGATTGG CTTCACCACA GACCCTCGGA TGGCCCGCTC TTCTCCCTAC 
CCCACTGATG TGGCCCGAGT GGTGAACGCC CCCATTTTCC ATGTCAACTC AGATGACCCT 
GAAGCTGTCA TGTATGTATG CAAGGTGGCA GCTGAGTGGA GAAACACCTT CCACAAGGAT 
GTTGTTGTTG ATCTGGTGTG TTATCGACGA AATGGCCACA ACGAGATGGA TGAACCTATG 
TTCACACAGC CACTCATGTA CAAGCAGATC CGTAAGCAGA AGCCGGTACT GCAGAAGTAT 
GCAGAATTGC TGGTATCCCA GGGGGTCGTC AACCAGCCCG AATATGAGGA GGAAATCTCC 
AAGTACGATA AGATCTGTGA GGAAGCATTT ACCAGATCCA AAGATGAGAA GATCTTGCAC 
ATCAAGCACT GGCTGGATTC CCCCTGGCCT GGCTTTTTCA CCCTGGATGG ACAGCCCAGG 
AGCATGACCT GCCCCTCCAC TGGCCTGGAG GAGGACATCT TGACCCACAT TGGGAATGTG 
GCCAGTTCTG TACCTGTGGA GAACTTTACC ATCCATGGAG GGCTGAGCCG GATCTTGAAG 
ACTCGCAGAG AGCTTGTGAC AAACCGGACT GTGGACTGGG CCCTGGCAGA GTACATGGCG 
TTCGGATCAC TCCTGAAGGA AGGCATCCAT GTCCGGCTGA GTGGCCAGGA CGTGGAGCGG 
GGTACCTTCA GCCATCGCCA CCATGTGCTC CATGACCAGA ATGTGGACAA AAGAACCTGC 
ATCCCCATGA ACCACCTTTG GCCTAACCAG GCCCCTTACA CCGTGTGCAA CAGCTCGTTG 
TCTGAGTATG GTGTCCTGGG CTTTGAACTG GGCTTTGCCA TGGCTAGCCC TAATGCTCTG 
GTTCTCTGGG AGGCCCAGTT TGGTGATTTC AACAACATGG CACAGTGCAT CATTGACCAG 
TTCATCTGCC CAGGACAGGC AAAGTGGGTG CGGCAGAATG GCATCGTGCT CCTGCTACCT 
CACGGCATGG AAGGCATGGG TCCCGAGCAT TCCTCTGCCC GTCCAGAGAG GTTTCTGCAG 
ATGTGCAATG ATGACCCAGA TGTCCTGCCT AACCTGCAGG AGGAAAACTT TGACATCAGT 
CAGCTCTACG ACTGCAACTG GATTGTCGTC AACTGCTCCA CCCCTGGCAA CTTCTTCCAC 
GTGCTGCGAC GACAGATCTT GCTGCCCTTC CGGAAGCCGT TAATCGTCTT CACTCCCAAA 
TCCCTCCTGC GCCACCCTGA GGCAAGAACT AGCTTTGATG AGATGCTGCC AGGAACCCAC 
TTCCAGCGTG TGATCCCAGA AGATGGACCT GCAGCTCAGA ACCCAGACAA AGTCAAGAGG 
CTTCTCTTCT GCACTGGCAA GGTGTACTAT GACCTCACCC GAGAGCGCAA AGCCAGGGAC 
ATGGCAGAGG AGGTGGCTAT TACAAGGATT GAGCAGCTGT CACCATTCCC CTTCGACCTC 
CTGCTGAAAG AGGCACAGAA GTATCCCAAT GCTGAGCTGG CCTGGTGCCA GGAAGAGCAC 
AAGAATCAAG GCTACTATGA CTATGTCAAG CCAAGACTTC GGACCACCAT TGACCGTGCT 
AAGCCTGTCT GGTATGCTGG CCGAGACCCA GCAGCCGCTC CAGCCACTGG CAACAAGAAG 
ACCCACCTGA CAGAGCTGCA GCGCTTTCTG GACACAGCCT TTGACCTGGA CGCCTTCAAG 
AAATTCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ogdh ( XM_006251448.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251510.1 Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa36377
Clone ID Related Accession (Same CDS sequence)	XM_006251448.3
Accession Version	XM_006251448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	2-oxoglutarate dehydrogenase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251448.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCATT TAAGGACTTG TGCTGCTAAG TTAAGGCCAT TGACAGCCTC CCAGACTGTT 
AAGACATTTT CACAAAACAA ACCAGCAGCA ATTAGGACGT TTCAACAGAT TCGGTGCTAT 
TCTGCACCTG TAGCTGCTGA ACCATTTCTT AGTGGGACTA GTTCGAACTA TGTGGAGGAA 
ATGTACTGTG CCTGGTTGGA GAATCCCAAA AGTGTACATA AGTCATGGGA CATTTTTTTC 
CGAAACACCA ATGCTGGAGC CCCACCGGGC ACTGCCTACC AGAGCCCCCT TTCCCTGAGT 
CGAAGCTCCC TGGCTACCAT GGCTCATGCA CAGTCCCTGG TGGAAGCACA GCCTAACGTC 
GACAAGCTCG TGGAGGACCA CTTGGCGGTG CAGTCTCTCA TCAGGGCGTA TCAGGTCAGG 
GGTCACCAAA TTGCAAAACT TGATCCTCTC GGAATTAGTT GTGTAAATTT TGATGATGCT 
CCGGTAACTG TTTCTTCAAA CGTGGGGTTC TATGGCCTAC ACGAGTCTGA CCTTGACAAG 
GTCTTCCACT TACCCACCAC CACTTTCATC GGGGGACAGG AGCCAGCACT TCCTCTTCGG 
GAGATCATCC GTCGGCTGGA GATGGCCTAC TGCCAGCACA TTGGTGTGGA GTTCATGTTC 
ATTAACGATT TGGAGCAATG CCAGTGGATC CGGCAGAAGT TTGAGACCCC TGGGATCATG 
CAGTTCACCA ATGAGGAGAA GCGGACCCTG CTGGCCAGGC TTGTACGGTC CACCAGGTTT 
GAGGAGTTCC TGCAGCGAAA GTGGTCCTCT GAGAAGCGTT TTGGTCTGGA AGGCTGTGAG 
GTGCTGATCC CTGCCCTCAA GACAATCATT GACATGTCAA GTGCAAATGG AGTGGACTAT 
GTGATCATGG GGATGCCACA CAGAGGACGA CTGAACGTGC TAGCCAATGT CATCAGGAAG 
GAGCTGGAGC AGATATTCTG TCAGTTTGAC TCGAAGCTGG AGGCAGCTGA TGAGGGTTCC 
GGGGACATGA AGTACCACCT GGGCATGTAT CACCGAAGGA TCAACCGTGT GACCGACAGA 
AACATCACTC TGTCCTTGGT GGCTAACCCT TCCCACTTAG AGGCTGCTGA CCCTGTAGTG 
ATGGGAAAGA CCAAAGCTGA ACAGTTCTAC TGTGGAGACA CTGAAGGGAA AAAGGTGATG 
TCTATCCTGC TGCATGGGGA TGCTGCCTTT GCTGGCCAGG GTATCGTGTA TGAGACCTTC 
CATCTCAGCG ACCTGCCATC CTATACAACC CACGGCACCG TTCACGTGGT TGTCAACAAC 
CAGATTGGCT TCACCACAGA CCCTCGGATG GCCCGCTCTT CTCCCTACCC CACTGATGTG 
GCCCGAGTGG TGAACGCCCC CATTTTCCAT GTCAACTCAG ATGACCCTGA AGCTGTCATG 
TATGTATGCA AGGTGGCAGC TGAGTGGAGA AACACCTTCC ACAAGGATGT TGTTGTTGAT 
CTGGTGTGTT ATCGACGAAA TGGCCACAAC GAGATGGATG AACCTATGTT CACACAGCCA 
CTCATGTACA AGCAGATCCG TAAGCAGAAG CCGGTACTGC AGAAGTATGC AGAATTGCTG 
GTATCCCAGG GGGTCGTCAA CCAGCCCGAA TATGAGGAGG AAATCTCCAA GTACGATAAG 
ATCTGTGAGG AAGCATTTAC CAGATCCAAA GATGAGAAGA TCTTGCACAT CAAGCACTGG 
CTGGATTCCC CCTGGCCTGG CTTTTTCACC CTGGATGGAC AGCCCAGGAG CATGACCTGC 
CCCTCCACTG GCCTGGAGGA GGACATCTTG ACCCACATTG GGAATGTGGC CAGTTCTGTA 
CCTGTGGAGA ACTTTACCAT CCATGGAGGG CTGAGCCGGA TCTTGAAGAC TCGCAGAGAG 
CTTGTGACAA ACCGGACTGT GGACTGGGCC CTGGCAGAGT ACATGGCGTT CGGATCACTC 
CTGAAGGAAG GCATCCATGT CCGGCTGAGT GGCCAGGACG TGGAGCGGGG TACCTTCAGC 
CATCGCCACC ATGTGCTCCA TGACCAGAAT GTGGACAAAA GAACCTGCAT CCCCATGAAC 
CACCTTTGGC CTAACCAGGC CCCTTACACC GTGTGCAACA GCTCGTTGTC TGAGTATGGT 
GTCCTGGGCT TTGAACTGGG CTTTGCCATG GCTAGCCCTA ATGCTCTGGT TCTCTGGGAG 
GCCCAGTTTG GTGATTTCAA CAACATGGCA CAGTGCATCA TTGACCAGTT CATCTGCCCA 
GGACAGGCAA AGTGGGTGCG GCAGAATGGC ATCGTGCTCC TGCTACCTCA CGGCATGGAA 
GGCATGGGTC CCGAGCATTC CTCTGCCCGT CCAGAGAGGT TTCTGCAGAT GTGCAATGAT 
GACCCAGATG TCCTGCCTAA CCTGCAGGAG GAAAACTTTG ACATCAGTCA GCTCTACGAC 
TGCAACTGGA TTGTCGTCAA CTGCTCCACC CCTGGCAACT TCTTCCACGT GCTGCGACGA 
CAGATCTTGC TGCCCTTCCG GAAGCCGTTA ATCGTCTTCA CTCCCAAATC CCTCCTGCGC 
CACCCTGAGG CAAGAACTAG CTTTGATGAG ATGCTGCCAG GAACCCACTT CCAGCGTGTG 
ATCCCAGAAG ATGGACCTGC AGCTCAGAAC CCAGACAAAG TCAAGAGGCT TCTCTTCTGC 
ACTGGCAAGG TGTACTATGA CCTCACCCGA GAGCGCAAAG CCAGGGACAT GGCAGAGGAG 
GTGGCTATTA CAAGGATTGA GCAGCTGTCA CCATTCCCCT TCGACCTCCT GCTGAAAGAG 
GCACAGAAGT ATCCCAATGC TGAGCTGGCC TGGTGCCAGG AAGAGCACAA GAATCAAGGC 
TACTATGACT ATGTCAAGCC AAGACTTCGG ACCACCATTG ACCGTGCTAA GCCTGTCTGG 
TATGCTGGCC GAGACCCAGC AGCCGCTCCA GCCACTGGCA ACAAGAAGAC CCACCTGACA 
GAGCTGCAGC GCTTTCTGGA CACAGCCTTT GACCTGGACG CCTTCAAGAA ATTCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251510.1
CDS	131..3190
Translation	MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRSSLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQVRGHQIAKLDPLGISCVNFDDAPVTVSSNVGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDMKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKICEEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDILTHIGNVASSVPVENFTIHGGLSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPNLQEENFDISQLYDCNWIVVNCSTPGNFFHVLRRQILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPEDGPAAQNPDKVKRLLFCTGKVYYDLTRERKARDMAEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRAKPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS

Target ORF information:

RefSeq Version	XM_006251448.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus oxoglutarate dehydrogenase (Ogdh), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251448.3

ORF Insert Sequence:

ATGTTTCATT TAAGGACTTG TGCTGCTAAG TTAAGGCCAT TGACAGCCTC CCAGACTGTT 
AAGACATTTT CACAAAACAA ACCAGCAGCA ATTAGGACGT TTCAACAGAT TCGGTGCTAT 
TCTGCACCTG TAGCTGCTGA ACCATTTCTT AGTGGGACTA GTTCGAACTA TGTGGAGGAA 
ATGTACTGTG CCTGGTTGGA GAATCCCAAA AGTGTACATA AGTCATGGGA CATTTTTTTC 
CGAAACACCA ATGCTGGAGC CCCACCGGGC ACTGCCTACC AGAGCCCCCT TTCCCTGAGT 
CGAAGCTCCC TGGCTACCAT GGCTCATGCA CAGTCCCTGG TGGAAGCACA GCCTAACGTC 
GACAAGCTCG TGGAGGACCA CTTGGCGGTG CAGTCTCTCA TCAGGGCGTA TCAGGTCAGG 
GGTCACCAAA TTGCAAAACT TGATCCTCTC GGAATTAGTT GTGTAAATTT TGATGATGCT 
CCGGTAACTG TTTCTTCAAA CGTGGGGTTC TATGGCCTAC ACGAGTCTGA CCTTGACAAG 
GTCTTCCACT TACCCACCAC CACTTTCATC GGGGGACAGG AGCCAGCACT TCCTCTTCGG 
GAGATCATCC GTCGGCTGGA GATGGCCTAC TGCCAGCACA TTGGTGTGGA GTTCATGTTC 
ATTAACGATT TGGAGCAATG CCAGTGGATC CGGCAGAAGT TTGAGACCCC TGGGATCATG 
CAGTTCACCA ATGAGGAGAA GCGGACCCTG CTGGCCAGGC TTGTACGGTC CACCAGGTTT 
GAGGAGTTCC TGCAGCGAAA GTGGTCCTCT GAGAAGCGTT TTGGTCTGGA AGGCTGTGAG 
GTGCTGATCC CTGCCCTCAA GACAATCATT GACATGTCAA GTGCAAATGG AGTGGACTAT 
GTGATCATGG GGATGCCACA CAGAGGACGA CTGAACGTGC TAGCCAATGT CATCAGGAAG 
GAGCTGGAGC AGATATTCTG TCAGTTTGAC TCGAAGCTGG AGGCAGCTGA TGAGGGTTCC 
GGGGACATGA AGTACCACCT GGGCATGTAT CACCGAAGGA TCAACCGTGT GACCGACAGA 
AACATCACTC TGTCCTTGGT GGCTAACCCT TCCCACTTAG AGGCTGCTGA CCCTGTAGTG 
ATGGGAAAGA CCAAAGCTGA ACAGTTCTAC TGTGGAGACA CTGAAGGGAA AAAGGTGATG 
TCTATCCTGC TGCATGGGGA TGCTGCCTTT GCTGGCCAGG GTATCGTGTA TGAGACCTTC 
CATCTCAGCG ACCTGCCATC CTATACAACC CACGGCACCG TTCACGTGGT TGTCAACAAC 
CAGATTGGCT TCACCACAGA CCCTCGGATG GCCCGCTCTT CTCCCTACCC CACTGATGTG 
GCCCGAGTGG TGAACGCCCC CATTTTCCAT GTCAACTCAG ATGACCCTGA AGCTGTCATG 
TATGTATGCA AGGTGGCAGC TGAGTGGAGA AACACCTTCC ACAAGGATGT TGTTGTTGAT 
CTGGTGTGTT ATCGACGAAA TGGCCACAAC GAGATGGATG AACCTATGTT CACACAGCCA 
CTCATGTACA AGCAGATCCG TAAGCAGAAG CCGGTACTGC AGAAGTATGC AGAATTGCTG 
GTATCCCAGG GGGTCGTCAA CCAGCCCGAA TATGAGGAGG AAATCTCCAA GTACGATAAG 
ATCTGTGAGG AAGCATTTAC CAGATCCAAA GATGAGAAGA TCTTGCACAT CAAGCACTGG 
CTGGATTCCC CCTGGCCTGG CTTTTTCACC CTGGATGGAC AGCCCAGGAG CATGACCTGC 
CCCTCCACTG GCCTGGAGGA GGACATCTTG ACCCACATTG GGAATGTGGC CAGTTCTGTA 
CCTGTGGAGA ACTTTACCAT CCATGGAGGG CTGAGCCGGA TCTTGAAGAC TCGCAGAGAG 
CTTGTGACAA ACCGGACTGT GGACTGGGCC CTGGCAGAGT ACATGGCGTT CGGATCACTC 
CTGAAGGAAG GCATCCATGT CCGGCTGAGT GGCCAGGACG TGGAGCGGGG TACCTTCAGC 
CATCGCCACC ATGTGCTCCA TGACCAGAAT GTGGACAAAA GAACCTGCAT CCCCATGAAC 
CACCTTTGGC CTAACCAGGC CCCTTACACC GTGTGCAACA GCTCGTTGTC TGAGTATGGT 
GTCCTGGGCT TTGAACTGGG CTTTGCCATG GCTAGCCCTA ATGCTCTGGT TCTCTGGGAG 
GCCCAGTTTG GTGATTTCAA CAACATGGCA CAGTGCATCA TTGACCAGTT CATCTGCCCA 
GGACAGGCAA AGTGGGTGCG GCAGAATGGC ATCGTGCTCC TGCTACCTCA CGGCATGGAA 
GGCATGGGTC CCGAGCATTC CTCTGCCCGT CCAGAGAGGT TTCTGCAGAT GTGCAATGAT 
GACCCAGATG TCCTGCCTAA CCTGCAGGAG GAAAACTTTG ACATCAGTCA GCTCTACGAC 
TGCAACTGGA TTGTCGTCAA CTGCTCCACC CCTGGCAACT TCTTCCACGT GCTGCGACGA 
CAGATCTTGC TGCCCTTCCG GAAGCCGTTA ATCGTCTTCA CTCCCAAATC CCTCCTGCGC 
CACCCTGAGG CAAGAACTAG CTTTGATGAG ATGCTGCCAG GAACCCACTT CCAGCGTGTG 
ATCCCAGAAG ATGGACCTGC AGCTCAGAAC CCAGACAAAG TCAAGAGGCT TCTCTTCTGC 
ACTGGCAAGG TGTACTATGA CCTCACCCGA GAGCGCAAAG CCAGGGACAT GGCAGAGGAG 
GTGGCTATTA CAAGGATTGA GCAGCTGTCA CCATTCCCCT TCGACCTCCT GCTGAAAGAG 
GCACAGAAGT ATCCCAATGC TGAGCTGGCC TGGTGCCAGG AAGAGCACAA GAATCAAGGC 
TACTATGACT ATGTCAAGCC AAGACTTCGG ACCACCATTG ACCGTGCTAA GCCTGTCTGG 
TATGCTGGCC GAGACCCAGC AGCCGCTCCA GCCACTGGCA ACAAGAAGAC CCACCTGACA 
GAGCTGCAGC GCTTTCTGGA CACAGCCTTT GACCTGGACG CCTTCAAGAA ATTCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KAT2A coupled with the ?-KGDH complex acts as a histone H3 succinyltransferase.
Nature552(7684)273-277(2017 Dec)
Wang Y,Guo YR,Liu K,Yin Z,Liu R,Xia Y,Tan L,Yang P,Lee JH,Li XJ,Hawke D,Zheng Y,Qian X,Lyu J,He J,Xing D,Tao YJ,Lu Z

Thiamine Induces Long-Term Changes in Amino Acid Profiles and Activities of 2-Oxoglutarate and 2-Oxoadipate Dehydrogenases in Rat Brain.
Biochemistry. Biokhimiia82(6)723-736(2017 Jun)
Tsepkova PM,Artiukhov AV,Boyko AI,Aleshin VA,Mkrtchyan GV,Zvyagintseva MA,Ryabov SI,Ksenofontov AL,Baratova LA,Graf AV,Bunik VI

Calcium-insensitive splice variants of mammalian E1 subunit of 2-oxoglutarate dehydrogenase complex with tissue-specific patterns of expression.
The Biochemical journal473(9)1165-78(2016 05)
Denton RM,Pullen TJ,Armstrong CT,Heesom KJ,Rutter GA

Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium-binding site.
The Biochemical journal459(2)369-81(2014 Apr)
Armstrong CT,Anderson JL,Denton RM

The 2-oxoacid dehydrogenase complexes in mitochondria can produce superoxide/hydrogen peroxide at much higher rates than complex I.
The Journal of biological chemistry289(12)8312-25(2014 Mar)
Quinlan CL,Goncalves RL,Hey-Mogensen M,Yadava N,Bunik VI,Brand MD