Tmem132a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmem132a
Entrez Gene ID	338474
Full Name	transmembrane protein 132A
Synonyms	Gbp,Hspa5bp1
General protein information	Preferred Names transmembrane protein 132A Names transmembrane protein 132A GRP78-binding protein HSPA5-binding protein 1 heat shock 70kDa protein 5 binding protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	ER/Golgi protein that negatively regulates serum starvation-induced cell death in culture; may be involved in brain development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178021.1	NP_821140.1	transmembrane protein 132A precursor
XM_006231041.3	XP_006231103.1	transmembrane protein 132A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMEM132A	NP_060340.2
Bos taurus (cattle)	TMEM132A	NP_001095707.1
Mus musculus (house mouse)	Tmem132a	NP_598565.2
Xenopus tropicalis (tropical clawed frog)	tmem132a	XP_002942160.1
Pan troglodytes (chimpanzee)	TMEM132A	XP_522023.3
Rattus norvegicus (Norway rat)	Tmem132a	NP_821140.1
Canis lupus familiaris (dog)	TMEM132A	XP_855179.2

Gene Ontology
Bibliography

Related articles in PubMed

Chelation of GRP78 with lead and its localization changes in the astroglia of rats exposed to lead.
Zhang Y, Ye L, Wang B, Li Y, Sun L
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban29(4)492-7(2009 Aug)

A 78-kDa glucose-regulated protein is involved in the decrease of interleukin-6 secretion by lead treatment from astrocytes.
Qian Y, Zheng Y, Weber D, Tiffany-Castiglioni E
American journal of physiology. Cell physiology293(3)C897-905(2007 Sep)

Albumin induces endoplasmic reticulum stress and apoptosis in renal proximal tubular cells.
Ohse T, Inagi R, Tanaka T, Ota T, Miyata T, Kojima I, Ingelfinger JR, Ogawa S, Fujita T, Nangaku M
Kidney international70(8)1447-55(2006 Oct)

GRP78-binding protein regulates cAMP-induced glial fibrillary acidic protein expression in rat C6 glioblastoma cells.
Oh-hashi K, Hirata Y, Koga H, Kiuchi K
FEBS letters580(16)3943-7(2006 Jul)

Cloning and characterization of a novel GRP78-binding protein in the rat brain.
Oh-hashi K, Naruse Y, Amaya F, Shimosato G, Tanaka M
The Journal of biological chemistry278(12)10531-7(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GRP78 in astroglia could strongly chelate with Pb ions.
Title: Chelation of GRP78 with lead and its localization changes in the astroglia of rats exposed to lead.

GRP78 is involved in IL-6 secretion from astrocytes, and lead can block IL-6 secretion from astrocytes via targeting GRP78
Title: A 78-kDa glucose-regulated protein is involved in the decrease of interleukin-6 secretion by lead treatment from astrocytes.

ER stress proteins (GRP78 and ORP150), respectively) were upregulated at PTCs in experimental proteinuric rats.
Title: Albumin induces endoplasmic reticulum stress and apoptosis in renal proximal tubular cells.

These results suggest a novel function of GBP in regulating GFAP gene expression via STAT3 phosphorylation.
Title: GRP78-binding protein regulates cAMP-induced glial fibrillary acidic protein expression in rat C6 glioblastoma cells.

cloning and characterization of GRP78-binding protein that plays a role in embryonic and postnatal development of the brain
Title: Cloning and characterization of a novel GRP78-binding protein in the rat brain.

The following Tmem132a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem132a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04121	NM_178021.1 Latest version!	Rattus norvegicus transmembrane protein 132A (Tmem132a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa23905	XM_006231041.3 Latest version!	Rattus norvegicus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmem132a ( NM_178021.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_821140.1 Rattus norvegicus transmembrane protein 132A (Tmem132a), mRNA.

Price & Availability↑

CloneID	ORa04121
Clone ID Related Accession (Same CDS sequence)	NM_178021.1
Accession Version	NM_178021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	transmembrane protein 132A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY216677.1. On Jan 12, 2005 this sequence version replaced XM_346562.1. ##Evidence-Data-START## Transcript exon combination :: AY216677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGACGGAAC GTGCGGCAGC GGCCCCTCGG GGGCCCTACG GTGCCTGGCT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT GGCAGTAACC AAAATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTGGAT GCCCCTGAAC ATTTTCGGGT GCAGCAGGTG 
GGCCACTACC CACCTGCCAA CTCTTCTCTG GGCTCCCGCT CTGAGACCTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCACTTCTC CGGGCCTCCT ACCCACCTTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACTGAGCCC CACCGGCGAC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAGCCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC TCACCAAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTAGTGGAAC TGCAGTTCCC CTCTCAATGG TTCTCCCAAA GTGCCACCAC AAGGGCAGAA 
CTGGCCTACA CTCTGGAGCC TGCAGGTGAG GGCCCTGGGG GCTGTGGCCC TGGCACGGAG 
GAGGAACCCA AGGAGCAGGC TCTTCCAGTG GGTAGTGTGG AGCTGCACCC TGAAGACCCC 
CCACAGCACC AGGAGGTCCC GCTGGATGAA GTAGTCACTT TACGTGCACC CGATGTGCCA 
GTGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAATTT CACAGCCAGC 
CTTCTTACTC TGCGGATCAA GGTGAAGAAA GGGCTACAGG TGACAGCTGC CCGCCCAGCC 
CAGCCCACCT TCTGGACCGC TAAGCTGGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGT 
CTGATCACCT GTCACCGCAC TGGGCCTGCA GGGCCGGATT CCAGGCCCCT GGAACTGCCC 
GAGTTCCTGT GGGTAGACTT TGCGGTGGAG AATAGCACCG GTGGGGGTGT GGCAGTCACT 
CGACCTGTCA CGTGGCAGCT GGAGTACCCA GGTCAGGCCC CTGAAGCAGA GAAGGACAAA 
ATGGTGTGGG AGATCCTGTT TTCTGAGCGG GACATCAGAG CCCTCATCCC TCTGGCCAAG 
GCTGAGGAGC TGGTGAACAC GGCTCCGTTG ACTGGAGTGC CACAGCGTAT CCCTGTGCGT 
CTTGTCACTG TGGACAGTGG GGGAGCCCTG GAGGAGGTGA CAGAGCACAT TGGCTGTGAA 
TCAGCCAATA CCCAGGTCCT GCAGGTATCT GAGGCCTGTG ATGCTGTGTT CGTGGCTGGT 
CAGGAGAGTC GGGGAGCCAA GGGGGTTCGG GTGGACTTCT GGTGGCGCCG CCTCCGGGCC 
TCACTGAAGC TGACTGTATG GGCCCCACTC CTACCCTTGC GCATTGAGCT GACTGACACC 
ACTCTTGAAC AAATCCGAGG CTGGCGGGTC CCAGGCCCAG CAGAAGGGCA GTTAGAGCCG 
GAGGCTGCTG CAGAGGAGGT GGAGCGGCGC TCCCGCGGCT GCCGCCTGCA GTACCAGAGA 
GCTGGAGTAC GCTTCTTAGT GCCCTTTGCG GCTCACCCGC TGGACGGTGG CCGCCGCCTC 
ACCCACCTAC TCGGTCCTGA CTGGTTGCTG GATGTGAGTC ACCTCGTGGC AGCCCATGCC 
CACGTGCAGG ATCCTCGCAT AGCCACCTTG GAAGGTGGCC GCATCCTAGT GGGACGAGAG 
CCTGGAGTCA CCTCTATCGA GGTTCGATCC CCGCTATCTG ACTCCATCCT GGGGGAGCAG 
GCACTGGCTG TGACAGATGA CAAGGTCTCG GTGCTGGATC TGCGAGTGCA ACCGGTGATG 
GGCATCTCAC TGTCCCTGAG CCGGGGCGTG TCCCACCCTG GAGAGGTCAC AGCGACTTGC 
TGGGCCCAGT CGGCTCTTCC AGCCCCAAAG CAGGAGGTGG CCCTCTCCCT GTGGCTGTCC 
TTTTCTGACC ACACTTTGGC TCCAGCCGAG CTCTACGACC GCAATGACCT GGGACTGTCG 
GTCTCAGCTG AGGAACCCAG TGCTGTTGTG CCAGCTGAAG AGCAGAGAGC CCAACTTGGT 
GTGGTGGTGA GTGGCGTGGG TGCCAAAGGG CTGCCCCTAC ACGTGGCTCT GCACCCACCG 
GAACCCTGCC GCCGGGGCCG CCATCGTGTT CCCCTAGCCT CAGGCACAGC CTGGCTGGGG 
CTGCCCCCTT TGCCCACACC AGCTCCTGCA CTCCCATCCA GCCCTGCCCG GACCCCACCA 
TTCACAGAAG CCTCTGTGGA AGGAAAGCGG CAGGTAGCAG GTGATATGGG GAGCCATGTG 
GGTCCGGGCA TTAGGGGCAA GTTTGAGCGG GCAGAGGAGG AAGCTGGGAA GGAAGAGAAT 
GAGGCCAAAG AAGAGGAGGA AGATGAGGAA GAGATGGTGC CTGCCCCTCA GCGTGTCACC 
GACCTAGAGC TGGGAATGTA TGCTCTGCTG GGCATCTTCT GTTTGGCCTT CCTCATCTTC 
CTGGTCAATG GTGTGGTCTT TGTGTTGCGC TACCAGCGCA AAGAGCCTCC CGACAGCGTT 
ACAGACCCTG CCTCCCCTCA GCCACATAAC TGGGTCTGGC TGGGCACCAA TCAGGAGGAA 
CTGAGTCGGC AGCTGGACCG ATGCTCTTCC TCGAGCCCAC CCAAGGGGGA GGGGGGCTGC 
CCCTGTGAAA GTGGGGCAGG AGGGGATACT TCAACCGTGG CTCCCAGTGC TTCAGAGAGC 
CCTGCTGGCT CCACGAGCAC CCTGGCCCGC AAAGAAGCTG GGGGACGTCG GAAGCGAGTC 
GAATTTGTGA CATTTGCACC AGCACCCCCA GCCCAGGAGG CGCCTGAGGA GCCTGTAGGG 
GCCCCGGCTG TACAGTCCAT CCTGGTAGCA GGTGAGGAGG ACATCCGCTG GGTGTGCGAG 
GACATGGGGC TGAAGGACCC AGAGGAGCTT CGGAACTACA TGGAGAGGAT CCGGGGCAGC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_821140.1
CDS	1..3066
Translation	MTERAAAAPRGPYGAWLCLLVALALEVVRVGSNQNTLDPIYLPVALELLDAPEHFRVQQVGHYPPANSSLGSRSETFLLMQPWPRAQPLLRASYPPFATQQVVPPRVTEPHRRPVPWDVRAVSVEAAVTPAEPYARVLFHLKGQDWPPGPGSLPCARLHATHPAGTAHQACRFQPSLGACVVELQFPSQWFSQSATTRAELAYTLEPAGEGPGGCGPGTEEEPKEQALPVGSVELHPEDPPQHQEVPLDEVVTLRAPDVPVRPGQLFTATLLLRHNFTASLLTLRIKVKKGLQVTAARPAQPTFWTAKLDRFKGSKHHTSLITCHRTGPAGPDSRPLELPEFLWVDFAVENSTGGGVAVTRPVTWQLEYPGQAPEAEKDKMVWEILFSERDIRALIPLAKAEELVNTAPLTGVPQRIPVRLVTVDSGGALEEVTEHIGCESANTQVLQVSEACDAVFVAGQESRGAKGVRVDFWWRRLRASLKLTVWAPLLPLRIELTDTTLEQIRGWRVPGPAEGQLEPEAAAEEVERRSRGCRLQYQRAGVRFLVPFAAHPLDGGRRLTHLLGPDWLLDVSHLVAAHAHVQDPRIATLEGGRILVGREPGVTSIEVRSPLSDSILGEQALAVTDDKVSVLDLRVQPVMGISLSLSRGVSHPGEVTATCWAQSALPAPKQEVALSLWLSFSDHTLAPAELYDRNDLGLSVSAEEPSAVVPAEEQRAQLGVVVSGVGAKGLPLHVALHPPEPCRRGRHRVPLASGTAWLGLPPLPTPAPALPSSPARTPPFTEASVEGKRQVAGDMGSHVGPGIRGKFERAEEEAGKEENEAKEEEEDEEEMVPAPQRVTDLELGMYALLGIFCLAFLIFLVNGVVFVLRYQRKEPPDSVTDPASPQPHNWVWLGTNQEELSRQLDRCSSSSPPKGEGGCPCESGAGGDTSTVAPSASESPAGSTSTLARKEAGGRRKRVEFVTFAPAPPAQEAPEEPVGAPAVQSILVAGEEDIRWVCEDMGLKDPEELRNYMERIRGSS

Target ORF information:

RefSeq Version	NM_178021.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transmembrane protein 132A (Tmem132a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178021.1

ORF Insert Sequence:

ATGACGGAAC GTGCGGCAGC GGCCCCTCGG GGGCCCTACG GTGCCTGGCT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT GGCAGTAACC AAAATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTGGAT GCCCCTGAAC ATTTTCGGGT GCAGCAGGTG 
GGCCACTACC CACCTGCCAA CTCTTCTCTG GGCTCCCGCT CTGAGACCTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCACTTCTC CGGGCCTCCT ACCCACCTTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACTGAGCCC CACCGGCGAC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAGCCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC TCACCAAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTAGTGGAAC TGCAGTTCCC CTCTCAATGG TTCTCCCAAA GTGCCACCAC AAGGGCAGAA 
CTGGCCTACA CTCTGGAGCC TGCAGGTGAG GGCCCTGGGG GCTGTGGCCC TGGCACGGAG 
GAGGAACCCA AGGAGCAGGC TCTTCCAGTG GGTAGTGTGG AGCTGCACCC TGAAGACCCC 
CCACAGCACC AGGAGGTCCC GCTGGATGAA GTAGTCACTT TACGTGCACC CGATGTGCCA 
GTGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAATTT CACAGCCAGC 
CTTCTTACTC TGCGGATCAA GGTGAAGAAA GGGCTACAGG TGACAGCTGC CCGCCCAGCC 
CAGCCCACCT TCTGGACCGC TAAGCTGGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGT 
CTGATCACCT GTCACCGCAC TGGGCCTGCA GGGCCGGATT CCAGGCCCCT GGAACTGCCC 
GAGTTCCTGT GGGTAGACTT TGCGGTGGAG AATAGCACCG GTGGGGGTGT GGCAGTCACT 
CGACCTGTCA CGTGGCAGCT GGAGTACCCA GGTCAGGCCC CTGAAGCAGA GAAGGACAAA 
ATGGTGTGGG AGATCCTGTT TTCTGAGCGG GACATCAGAG CCCTCATCCC TCTGGCCAAG 
GCTGAGGAGC TGGTGAACAC GGCTCCGTTG ACTGGAGTGC CACAGCGTAT CCCTGTGCGT 
CTTGTCACTG TGGACAGTGG GGGAGCCCTG GAGGAGGTGA CAGAGCACAT TGGCTGTGAA 
TCAGCCAATA CCCAGGTCCT GCAGGTATCT GAGGCCTGTG ATGCTGTGTT CGTGGCTGGT 
CAGGAGAGTC GGGGAGCCAA GGGGGTTCGG GTGGACTTCT GGTGGCGCCG CCTCCGGGCC 
TCACTGAAGC TGACTGTATG GGCCCCACTC CTACCCTTGC GCATTGAGCT GACTGACACC 
ACTCTTGAAC AAATCCGAGG CTGGCGGGTC CCAGGCCCAG CAGAAGGGCA GTTAGAGCCG 
GAGGCTGCTG CAGAGGAGGT GGAGCGGCGC TCCCGCGGCT GCCGCCTGCA GTACCAGAGA 
GCTGGAGTAC GCTTCTTAGT GCCCTTTGCG GCTCACCCGC TGGACGGTGG CCGCCGCCTC 
ACCCACCTAC TCGGTCCTGA CTGGTTGCTG GATGTGAGTC ACCTCGTGGC AGCCCATGCC 
CACGTGCAGG ATCCTCGCAT AGCCACCTTG GAAGGTGGCC GCATCCTAGT GGGACGAGAG 
CCTGGAGTCA CCTCTATCGA GGTTCGATCC CCGCTATCTG ACTCCATCCT GGGGGAGCAG 
GCACTGGCTG TGACAGATGA CAAGGTCTCG GTGCTGGATC TGCGAGTGCA ACCGGTGATG 
GGCATCTCAC TGTCCCTGAG CCGGGGCGTG TCCCACCCTG GAGAGGTCAC AGCGACTTGC 
TGGGCCCAGT CGGCTCTTCC AGCCCCAAAG CAGGAGGTGG CCCTCTCCCT GTGGCTGTCC 
TTTTCTGACC ACACTTTGGC TCCAGCCGAG CTCTACGACC GCAATGACCT GGGACTGTCG 
GTCTCAGCTG AGGAACCCAG TGCTGTTGTG CCAGCTGAAG AGCAGAGAGC CCAACTTGGT 
GTGGTGGTGA GTGGCGTGGG TGCCAAAGGG CTGCCCCTAC ACGTGGCTCT GCACCCACCG 
GAACCCTGCC GCCGGGGCCG CCATCGTGTT CCCCTAGCCT CAGGCACAGC CTGGCTGGGG 
CTGCCCCCTT TGCCCACACC AGCTCCTGCA CTCCCATCCA GCCCTGCCCG GACCCCACCA 
TTCACAGAAG CCTCTGTGGA AGGAAAGCGG CAGGTAGCAG GTGATATGGG GAGCCATGTG 
GGTCCGGGCA TTAGGGGCAA GTTTGAGCGG GCAGAGGAGG AAGCTGGGAA GGAAGAGAAT 
GAGGCCAAAG AAGAGGAGGA AGATGAGGAA GAGATGGTGC CTGCCCCTCA GCGTGTCACC 
GACCTAGAGC TGGGAATGTA TGCTCTGCTG GGCATCTTCT GTTTGGCCTT CCTCATCTTC 
CTGGTCAATG GTGTGGTCTT TGTGTTGCGC TACCAGCGCA AAGAGCCTCC CGACAGCGTT 
ACAGACCCTG CCTCCCCTCA GCCACATAAC TGGGTCTGGC TGGGCACCAA TCAGGAGGAA 
CTGAGTCGGC AGCTGGACCG ATGCTCTTCC TCGAGCCCAC CCAAGGGGGA GGGGGGCTGC 
CCCTGTGAAA GTGGGGCAGG AGGGGATACT TCAACCGTGG CTCCCAGTGC TTCAGAGAGC 
CCTGCTGGCT CCACGAGCAC CCTGGCCCGC AAAGAAGCTG GGGGACGTCG GAAGCGAGTC 
GAATTTGTGA CATTTGCACC AGCACCCCCA GCCCAGGAGG CGCCTGAGGA GCCTGTAGGG 
GCCCCGGCTG TACAGTCCAT CCTGGTAGCA GGTGAGGAGG ACATCCGCTG GGTGTGCGAG 
GACATGGGGC TGAAGGACCC AGAGGAGCTT CGGAACTACA TGGAGAGGAT CCGGGGCAGC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem132a ( XM_006231041.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231103.1 Rattus norvegicus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23905
Clone ID Related Accession (Same CDS sequence)	XM_006231041.3
Accession Version	XM_006231041.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transmembrane protein 132A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231041.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAC GTGCGGCAGC GGCCCCTCGG GGGCCCTACG GTGCCTGGCT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT GGCAGTAACC AAGATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTGGAT GCCCCTGAAC ATTTTCGGGT GCAGCAGGTG 
GGCCACTACC CACCTGCCAA CTCTTCTCTG GGCTCCCGCT CTGAGACCTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCACTTCTC CGGGCCTCCT ACCCACCTTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACTGAGCCC CACCGGCGAC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAGCCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC TCACCAAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTAGTGGAAC TGGAGTTCCC CTCTCAATGG TTCTCCCAAA GTGCCACCAC AAGGGCAGAA 
CTGGCCTACA CTCTGGAGCC TGCAGGTGAG GGCCCTGGGG GCTGTGGCCC TGGCACGGAG 
GAGGAACCCA AGGAGCAGGC TCTTCCAGTG GGTAGTGTGG AGCTGCACCC TGAAGACCCC 
CCACAGTACC AGGAGGTCCC GCTGGATGAA GTAGTCACTT TACGTGCACC CGATGTGCCA 
GTGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAATTT CACAGCCAGC 
CTTCTTACTC TGCGGATCAA GGTGAAGAAA GGGCTACAGG TGACAGCTGC CCGCCCAGCC 
CAGCCCACCT TCTGGACCGC TAAGCTGGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGT 
CTGATCACCT GTCACCGCAC TGGGCCTGCA GGGCCGGATT CCAGTAGTCC CCTGGAGCTG 
TCCGAGTTCC TGTGGGTAGA CTTTGCGGTG GAGAATAGCA CCGGTGGGGG TGTGGCAGTC 
ACTCGACCTG TCACGTGGCA GCTGGAGTAC CCAGGTCAGG CCCCTGAAGC AGAGAAGGAC 
AAAATGGTGT GGGAGATCCT GGTGTCTGAG CGGGACATCA GAGCCCTCAT CCCTCTGGCC 
AAGGCTGAGG AGCTGGTGAA CACGGCTCCC TTGACTGGAG TGCCACAGCG TATCCCTGTG 
CGTCTTGTCA CTGTGGACAG TGGGGGAGCC CTGGAGGAGG TGACAGAGCA CATTGGCTGT 
GAATCAGCCA ATACCCAGGT CCTGCAGGTA TCTGAGGCCT GTGATGCTGT GTTCGTGGCT 
GGTCAGGAGA GTCGGGGAGC CAAGGGGGTG CGGGTGGACT TCTGGTGGCG CCGCCTCCGG 
GCCTCACTGA AGCTGACTGT ATGGGCCCCA CTCCTACCCT TGCGCATTGA GCTGACTGAC 
ACCACTCTTG AACAAATCCG AGGCTGGCGG GTCCCAGGCC CAGCAGAAGG GCAGTTAGAG 
CCGGAGGCTG CTGCTGAGGA AGTGGAGCGG CGCTCCCGCG GCTGCCGCCT GCAGTACCAG 
AGAGCTGGTG TACGCTTCTT AGTGCCCTTT GCGGCTCACC CGCTGGACGG TGGCCGCCGC 
CTCACCCACC TACTCGGTCC TGACTGGTTG CTGGATGTGA GTCACCTCGT GGCAGCCCAT 
GCCCACGTGC AGGATCCTCG CATAGCCACC TTGGAAGGTG GCCGCATCCT AGTGGGACGA 
GAGCCTGGAG TCACCTCTAT CGAGGTTCGA TCCCCGCTAT CTGACTCCAT CCTGGGGGAG 
CAGGCACTGG CTGTGACAGA TGACAAGGTC TCGGTGCTGG ATCTGCGAGT GCAACCGGTG 
ATGGGCATCT CACTGTCCCT GAGCCGGGGC GTGTCCCACC CTGGAGAGGT CACAGCGACT 
TGCTGGGCCC AGTCAGCTCT TCCAGCCCCA AAGCAGGAGG TGGCCCTCTC CCTGTGGCTG 
TCCTTTTCTG ACCACACTTT GGCTCCAGCC GAGCTCTACG ACCGCAATGA CCTGGGACTG 
TCGGTCTCAG CTGAGGAACC CAGTGCTGTT GTGCCAGCTG AAGAGCAGAG AGCCCAACTT 
GGTGTGGTGG TGAGTGGCGT GGGTGCCGAA GGGCTGCCCC TACACGTGGC TCTGCACCCA 
CCGGAACCCT GCCGCCGGGG CCGCCATCGT GTTCCCCTAG CCTCAGGCAC AGCCTGGCTG 
GGGCTGCCCC CTTTGCCCAC ACCAGCTCCT GCACTCCCAT CCAGCCCTGC CCGGACCCCA 
CCATTCACAG AAGCCTCTGT GGAAGGAAAG CGGCAGGTAG CAGGTGATAT GGGGAGCCAT 
GTGGGTCCGG GCATTAGGGG CAAGTTTGAG CGGGCAGAGG AGGAAGCTGG GAAGGAAGAG 
AATGAGGCCA AAGAAGAGGA GGAAGATGAG GAAGAGATGG TGCCTGCCCC TCAGCGTGTC 
ACCGACCTAG AGCTGGGAAT GTATGCTCTG CTGGGCATCT TCTGTTTGGC CTTCCTCATC 
TTCCTGGTCA ATGGTGTGGT CTTTGTGTTG CGCTACCAGC GCAAAGAGCC TCCCGACAGC 
GCTACAGACC CTGCCTCCCC TCAGCCACAT AACTGGGTCT GGCTGGGCAC CAATCAGGAG 
GAACTGAGTC GGCAGCTGGA CCGATGCTCT TCCTCGAGCC CACCCAAGGG GGAGGGGGGC 
TGCCCCTGTG AAAGTGGGGC AGGAGGGGAT ACTTCAACCG TGGCTCCCAG TGCTTCAGAG 
AGCCCTGCTG GCTCCTCGAG CACCCTGGCC CGCAAAGAAG CTGGGGGACG TCGGAAGCGA 
GTCGAATTTG TGACATTTGC ACCAGCACCC CCAGCCCAGG AGGTGCCTGA GGAGCCTGTA 
GGGGCCCCGG CTGTACAGTC CATCCTGGTA GCAGGTGAGG AGGACATCCG CTGGGTGTGC 
GAGGACATGG GGCTGAAGGA CCCAGAGGAG CTTCGGAACT ACATGGAGAG GATCCGGGGC 
AGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231103.1
CDS	924..3992
Translation	MTERAAAAPRGPYGAWLCLLVALALEVVRVGSNQDTLDPIYLPVALELLDAPEHFRVQQVGHYPPANSSLGSRSETFLLMQPWPRAQPLLRASYPPFATQQVVPPRVTEPHRRPVPWDVRAVSVEAAVTPAEPYARVLFHLKGQDWPPGPGSLPCARLHATHPAGTAHQACRFQPSLGACVVELEFPSQWFSQSATTRAELAYTLEPAGEGPGGCGPGTEEEPKEQALPVGSVELHPEDPPQYQEVPLDEVVTLRAPDVPVRPGQLFTATLLLRHNFTASLLTLRIKVKKGLQVTAARPAQPTFWTAKLDRFKGSKHHTSLITCHRTGPAGPDSSSPLELSEFLWVDFAVENSTGGGVAVTRPVTWQLEYPGQAPEAEKDKMVWEILVSERDIRALIPLAKAEELVNTAPLTGVPQRIPVRLVTVDSGGALEEVTEHIGCESANTQVLQVSEACDAVFVAGQESRGAKGVRVDFWWRRLRASLKLTVWAPLLPLRIELTDTTLEQIRGWRVPGPAEGQLEPEAAAEEVERRSRGCRLQYQRAGVRFLVPFAAHPLDGGRRLTHLLGPDWLLDVSHLVAAHAHVQDPRIATLEGGRILVGREPGVTSIEVRSPLSDSILGEQALAVTDDKVSVLDLRVQPVMGISLSLSRGVSHPGEVTATCWAQSALPAPKQEVALSLWLSFSDHTLAPAELYDRNDLGLSVSAEEPSAVVPAEEQRAQLGVVVSGVGAEGLPLHVALHPPEPCRRGRHRVPLASGTAWLGLPPLPTPAPALPSSPARTPPFTEASVEGKRQVAGDMGSHVGPGIRGKFERAEEEAGKEENEAKEEEEDEEEMVPAPQRVTDLELGMYALLGIFCLAFLIFLVNGVVFVLRYQRKEPPDSATDPASPQPHNWVWLGTNQEELSRQLDRCSSSSPPKGEGGCPCESGAGGDTSTVAPSASESPAGSSSTLARKEAGGRRKRVEFVTFAPAPPAQEVPEEPVGAPAVQSILVAGEEDIRWVCEDMGLKDPEELRNYMERIRGSS

Target ORF information:

RefSeq Version	XM_006231041.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231041.3

ORF Insert Sequence:

ATGACGGAAC GTGCGGCAGC GGCCCCTCGG GGGCCCTACG GTGCCTGGCT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT GGCAGTAACC AAGATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTGGAT GCCCCTGAAC ATTTTCGGGT GCAGCAGGTG 
GGCCACTACC CACCTGCCAA CTCTTCTCTG GGCTCCCGCT CTGAGACCTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCACTTCTC CGGGCCTCCT ACCCACCTTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACTGAGCCC CACCGGCGAC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAGCCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC TCACCAAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTAGTGGAAC TGGAGTTCCC CTCTCAATGG TTCTCCCAAA GTGCCACCAC AAGGGCAGAA 
CTGGCCTACA CTCTGGAGCC TGCAGGTGAG GGCCCTGGGG GCTGTGGCCC TGGCACGGAG 
GAGGAACCCA AGGAGCAGGC TCTTCCAGTG GGTAGTGTGG AGCTGCACCC TGAAGACCCC 
CCACAGTACC AGGAGGTCCC GCTGGATGAA GTAGTCACTT TACGTGCACC CGATGTGCCA 
GTGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAATTT CACAGCCAGC 
CTTCTTACTC TGCGGATCAA GGTGAAGAAA GGGCTACAGG TGACAGCTGC CCGCCCAGCC 
CAGCCCACCT TCTGGACCGC TAAGCTGGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGT 
CTGATCACCT GTCACCGCAC TGGGCCTGCA GGGCCGGATT CCAGTAGTCC CCTGGAGCTG 
TCCGAGTTCC TGTGGGTAGA CTTTGCGGTG GAGAATAGCA CCGGTGGGGG TGTGGCAGTC 
ACTCGACCTG TCACGTGGCA GCTGGAGTAC CCAGGTCAGG CCCCTGAAGC AGAGAAGGAC 
AAAATGGTGT GGGAGATCCT GGTGTCTGAG CGGGACATCA GAGCCCTCAT CCCTCTGGCC 
AAGGCTGAGG AGCTGGTGAA CACGGCTCCC TTGACTGGAG TGCCACAGCG TATCCCTGTG 
CGTCTTGTCA CTGTGGACAG TGGGGGAGCC CTGGAGGAGG TGACAGAGCA CATTGGCTGT 
GAATCAGCCA ATACCCAGGT CCTGCAGGTA TCTGAGGCCT GTGATGCTGT GTTCGTGGCT 
GGTCAGGAGA GTCGGGGAGC CAAGGGGGTG CGGGTGGACT TCTGGTGGCG CCGCCTCCGG 
GCCTCACTGA AGCTGACTGT ATGGGCCCCA CTCCTACCCT TGCGCATTGA GCTGACTGAC 
ACCACTCTTG AACAAATCCG AGGCTGGCGG GTCCCAGGCC CAGCAGAAGG GCAGTTAGAG 
CCGGAGGCTG CTGCTGAGGA AGTGGAGCGG CGCTCCCGCG GCTGCCGCCT GCAGTACCAG 
AGAGCTGGTG TACGCTTCTT AGTGCCCTTT GCGGCTCACC CGCTGGACGG TGGCCGCCGC 
CTCACCCACC TACTCGGTCC TGACTGGTTG CTGGATGTGA GTCACCTCGT GGCAGCCCAT 
GCCCACGTGC AGGATCCTCG CATAGCCACC TTGGAAGGTG GCCGCATCCT AGTGGGACGA 
GAGCCTGGAG TCACCTCTAT CGAGGTTCGA TCCCCGCTAT CTGACTCCAT CCTGGGGGAG 
CAGGCACTGG CTGTGACAGA TGACAAGGTC TCGGTGCTGG ATCTGCGAGT GCAACCGGTG 
ATGGGCATCT CACTGTCCCT GAGCCGGGGC GTGTCCCACC CTGGAGAGGT CACAGCGACT 
TGCTGGGCCC AGTCAGCTCT TCCAGCCCCA AAGCAGGAGG TGGCCCTCTC CCTGTGGCTG 
TCCTTTTCTG ACCACACTTT GGCTCCAGCC GAGCTCTACG ACCGCAATGA CCTGGGACTG 
TCGGTCTCAG CTGAGGAACC CAGTGCTGTT GTGCCAGCTG AAGAGCAGAG AGCCCAACTT 
GGTGTGGTGG TGAGTGGCGT GGGTGCCGAA GGGCTGCCCC TACACGTGGC TCTGCACCCA 
CCGGAACCCT GCCGCCGGGG CCGCCATCGT GTTCCCCTAG CCTCAGGCAC AGCCTGGCTG 
GGGCTGCCCC CTTTGCCCAC ACCAGCTCCT GCACTCCCAT CCAGCCCTGC CCGGACCCCA 
CCATTCACAG AAGCCTCTGT GGAAGGAAAG CGGCAGGTAG CAGGTGATAT GGGGAGCCAT 
GTGGGTCCGG GCATTAGGGG CAAGTTTGAG CGGGCAGAGG AGGAAGCTGG GAAGGAAGAG 
AATGAGGCCA AAGAAGAGGA GGAAGATGAG GAAGAGATGG TGCCTGCCCC TCAGCGTGTC 
ACCGACCTAG AGCTGGGAAT GTATGCTCTG CTGGGCATCT TCTGTTTGGC CTTCCTCATC 
TTCCTGGTCA ATGGTGTGGT CTTTGTGTTG CGCTACCAGC GCAAAGAGCC TCCCGACAGC 
GCTACAGACC CTGCCTCCCC TCAGCCACAT AACTGGGTCT GGCTGGGCAC CAATCAGGAG 
GAACTGAGTC GGCAGCTGGA CCGATGCTCT TCCTCGAGCC CACCCAAGGG GGAGGGGGGC 
TGCCCCTGTG AAAGTGGGGC AGGAGGGGAT ACTTCAACCG TGGCTCCCAG TGCTTCAGAG 
AGCCCTGCTG GCTCCTCGAG CACCCTGGCC CGCAAAGAAG CTGGGGGACG TCGGAAGCGA 
GTCGAATTTG TGACATTTGC ACCAGCACCC CCAGCCCAGG AGGTGCCTGA GGAGCCTGTA 
GGGGCCCCGG CTGTACAGTC CATCCTGGTA GCAGGTGAGG AGGACATCCG CTGGGTGTGC 
GAGGACATGG GGCTGAAGGA CCCAGAGGAG CTTCGGAACT ACATGGAGAG GATCCGGGGC 
AGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chelation of GRP78 with lead and its localization changes in the astroglia of rats exposed to lead.
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban29(4)492-7(2009 Aug)
Zhang Y,Ye L,Wang B,Li Y,Sun L

A 78-kDa glucose-regulated protein is involved in the decrease of interleukin-6 secretion by lead treatment from astrocytes.
American journal of physiology. Cell physiology293(3)C897-905(2007 Sep)
Qian Y,Zheng Y,Weber D,Tiffany-Castiglioni E

Albumin induces endoplasmic reticulum stress and apoptosis in renal proximal tubular cells.
Kidney international70(8)1447-55(2006 Oct)
Ohse T,Inagi R,Tanaka T,Ota T,Miyata T,Kojima I,Ingelfinger JR,Ogawa S,Fujita T,Nangaku M

GRP78-binding protein regulates cAMP-induced glial fibrillary acidic protein expression in rat C6 glioblastoma cells.
FEBS letters580(16)3943-7(2006 Jul)
Oh-hashi K,Hirata Y,Koga H,Kiuchi K

Cloning and characterization of a novel GRP78-binding protein in the rat brain.
The Journal of biological chemistry278(12)10531-7(2003 Mar)
Oh-hashi K,Naruse Y,Amaya F,Shimosato G,Tanaka M