Racgap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Racgap1
Entrez Gene ID	315298
Full Name	Rac GTPase-activating protein 1
General protein information	Preferred Names Rac GTPase-activating protein 1 Names rac GTPase-activating protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594892.1	XP_017450381.1	rac GTPase-activating protein 1 isoform X1
XM_006257400.3	XP_006257462.1	rac GTPase-activating protein 1 isoform X1
NM_001108112.1	NP_001101582.1	rac GTPase-activating protein 1

Pathways from BioSystems

Homologies

	RACGAP1	XP_001110799.1
Rattus norvegicus (Norway rat)	Racgap1	NP_001101582.1
Homo sapiens (human)	RACGAP1	NP_001119575.1
Pan troglodytes (chimpanzee)	RACGAP1	XP_001156681.1
Bos taurus (cattle)	RACGAP1	XP_005206410.1
Danio rerio (zebrafish)	racgap1	NP_955925.1
Xenopus tropicalis (tropical clawed frog)	racgap1	NP_001001236.1
Mus musculus (house mouse)	Racgap1	NP_001240737.1
Rattus norvegicus (Norway rat)	LOC100909901	XP_003752175.1
Gallus gallus (chicken)	RACGAP1	XP_004949789.1
Drosophila melanogaster (fruit fly)	tum	NP_610912.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008912	XP_319660.4
Canis lupus familiaris (dog)	RACGAP1	XP_543675.2

Gene Ontology
Bibliography

Related articles in PubMed

Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis.
Lekomtsev S, Su KC, Pye VE, Blight K, Sundaramoorthy S, Takaki T, Collinson LM, Cherepanov P, Divecha N, Petronczki M
Nature492(7428)276-9(2012 Dec)

Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells.
Burkard ME, Maciejowski J, Rodriguez-Bravo V, Repka M, Lowery DM, Clauser KR, Zhang C, Shokat KM, Carr SA, Yaffe MB, Jallepalli PV
PLoS biology7(5)e1000111(2009 May)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis.
Simon GC, Schonteich E, Wu CC, Piekny A, Ekiert D, Yu X, Gould GW, Glotzer M, Prekeris R
The EMBO journal27(13)1791-803(2008 Jul)

Dissecting the role of Rho-mediated signaling in contractile ring formation.
Kamijo K, Ohara N, Abe M, Uchimura T, Hosoya H, Lee JS, Miki T
Molecular biology of the cell17(1)43-55(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Racgap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Racgap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06699	NM_001108112.1 Latest version!	Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa06699	XM_017594892.1 Latest version!	Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa06699	XM_006257400.3 Latest version!	Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Racgap1 ( NM_001108112.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101582.1 Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), mRNA.

Price & Availability↑

CloneID	ORa06699
Clone ID Related Accession (Same CDS sequence)	XM_017594892.1 , XM_006257400.3 , NM_001108112.1
Accession Version	NM_001108112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	rac GTPase-activating protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474035.2. On or before Oct 4, 2007 this sequence version replaced XM_235650.4, XM_001062413.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGATACTA CAATGGTGAA TCTGTGGACT CTGTTTGAGC AGCTTGTGCG CCGGCTGGAG 
ATTATCAATG AAGGAAATGA AAGCATTGAA TTCATCCAGG TTGTAAAGGA CTTCGAGGAC 
TTCCGAAAGA AATGTCAAAG AACCAACCAG GAGCTGGAGA AATTCAAAGA TCTGTTGTTG 
AAGGCAGAGA CTGGGCGGAG TGCCCTGGAC GTCAAGCTGA AGCACGCCCG TAACCAAGTG 
GATGTGGAGA TCAAACGGAG GCAACGAGCT GAGGCCGAGT GTGCGAAGCT GGAACAACAG 
ATTCAGCTGA TTCGAGATAT ACTCATGTGT GACACAACTG GCAGCATCCA GCTAAGCGAG 
GAACAAAAAT CAGCTCTGGC TTTTCTCAAC CGAGGCCAAG CATCCAGTGG CAACGCTGGG 
AACAATAGAC TGTCAACGAT TGATGAATCT GGCTCCATTT TATCAGATAT CAGCTTTGAC 
AAGACTGATG AATCACTGGA CTGGGATTCT TCTTTGAAGA ATTTCAAAAT GAAGAAACGA 
GAAAAGAGGC GCTCCAGCAG TAGACAGTTC ATCGATGGCC CTCCTGGGCC TGTGAAGAAA 
ACGTGTTCCA TTGGCTCTAC AATAGACCAG GCAAATGAAT CAATAGTTGC TAAAACAACG 
GTGACTGTCC CCAGTGACGG TGGGCCCATT GAAGCCGTGT CCACTATCGA GACATTGCCG 
TGTTGGACCA GGAGTCGAGG GAAATCAGGA CCTTTACAAC CTGTGAACAG CGACTCTGCT 
CTCAATAGCA GGCCACTGGA GCCAAGAACT GAGACAGACG CTGTGGGCAC ACCCCAGAAT 
AATGGCATGC GCTTACACGA CTTTGTCTCA AAGACGGTTA TTAAGCCTGA ATCTTGTGTC 
CCGTGTGGGA AGCGGATCAA GTTTGGCAAG CTGTCTCTGA AGTGTCGGGA CTGTCGTTTG 
GTCTCTCATC CAGAATGCCG GGACCGATGT CCCCTTCCCT GCATTCCTCC CCTGGTGGGA 
ACCCCCGTGA AGATTGGAGA GGGAATGCTG GCAGATTTTG TGTCGCAGAC TTCCCCAATG 
ATTCCTGCTA TTGTTGTCAG CTGTGTGAAT GAGATTGAGC AGCGAGGCCT GACTGAGGCA 
GGCTTATACA GGATCTCAGG CTGTGACCGG ACAGTAAAGG AGCTGAAAGA GAAATTCCTT 
AAAGTGAAAA CCGTGCCCCT CCTCAGCAAA GTGGACGACA TCCATGCCAT CTGCAGCCTC 
CTGAAAGACT TCCTTCGAAA CCTCAAGGAA CCCCTTCTGA CCTTTTGGCT AAGCAAAGCG 
TTCATGGAGG CAGCAGAGAT AACGGATGAA GACAACAGCA CAGCCGCCAT GTACCAGGCT 
GTCAGTGAGC TGCCCCAGGC CAACAGGGAC ACGCTAGCCT TCCTCATGAT CCACCTCCAG 
AGAGTGGCTC AGAGTCCGAG CACTAAGATG GACGTTGTCA ATCTGGCTAA AATCTTCGGC 
CCTACGATAG TTGCCCACAC TGTGCCCAAT CCAGATCCAG TGACGATGTT CCAGGACATC 
AAACGCCAGC TCAAGGTGGT GGAGCGCCTG CTCTCTCTGC CCCTGGAGTA CTGGAATCAG 
TTCATGATGG TGGAACAAGA GAACATGGAC TGCCACCGCG GCAATGGGAG CTCAACACCA 
CGGACACCAG ACATTAAAGT GAGCTTATTG GGGCCTGTGA CCACTCCTGA ATTCCAGCTT 
GTCAAGACTC CTTCATCAAA TTCCCTGTCA CAGAGATTGT ACAACCTCTC CAAGAGCACA 
CCCAGATTTG GGAACAAGAG CAAGTCTGCC ACCAACTTAG GTCAACAGGG CAAATTTTTC 
CCTGCTCCGT ACCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101582.1
CDS	182..2062
Translation	MDTTMVNLWTLFEQLVRRLEIINEGNESIEFIQVVKDFEDFRKKCQRTNQELEKFKDLLLKAETGRSALDVKLKHARNQVDVEIKRRQRAEAECAKLEQQIQLIRDILMCDTTGSIQLSEEQKSALAFLNRGQASSGNAGNNRLSTIDESGSILSDISFDKTDESLDWDSSLKNFKMKKREKRRSSSRQFIDGPPGPVKKTCSIGSTIDQANESIVAKTTVTVPSDGGPIEAVSTIETLPCWTRSRGKSGPLQPVNSDSALNSRPLEPRTETDAVGTPQNNGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRLVSHPECRDRCPLPCIPPLVGTPVKIGEGMLADFVSQTSPMIPAIVVSCVNEIEQRGLTEAGLYRISGCDRTVKELKEKFLKVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFWLSKAFMEAAEITDEDNSTAAMYQAVSELPQANRDTLAFLMIHLQRVAQSPSTKMDVVNLAKIFGPTIVAHTVPNPDPVTMFQDIKRQLKVVERLLSLPLEYWNQFMMVEQENMDCHRGNGSSTPRTPDIKVSLLGPVTTPEFQLVKTPSSNSLSQRLYNLSKSTPRFGNKSKSATNLGQQGKFFPAPYLK

Target ORF information:

RefSeq Version	NM_001108112.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108112.1

ORF Insert Sequence:

ATGGATACTA CAATGGTGAA TCTGTGGACT CTGTTTGAGC AGCTTGTGCG CCGGCTGGAG 
ATTATCAATG AAGGAAATGA AAGCATTGAA TTCATCCAGG TTGTAAAGGA CTTCGAGGAC 
TTCCGAAAGA AATGTCAAAG AACCAACCAG GAGCTGGAGA AATTCAAAGA TCTGTTGTTG 
AAGGCAGAGA CTGGGCGGAG TGCCCTGGAC GTCAAGCTGA AGCACGCCCG TAACCAAGTG 
GATGTGGAGA TCAAACGGAG GCAACGAGCT GAGGCCGAGT GTGCGAAGCT GGAACAACAG 
ATTCAGCTGA TTCGAGATAT ACTCATGTGT GACACAACTG GCAGCATCCA GCTAAGCGAG 
GAACAAAAAT CAGCTCTGGC TTTTCTCAAC CGAGGCCAAG CATCCAGTGG CAACGCTGGG 
AACAATAGAC TGTCAACGAT TGATGAATCT GGCTCCATTT TATCAGATAT CAGCTTTGAC 
AAGACTGATG AATCACTGGA CTGGGATTCT TCTTTGAAGA ATTTCAAAAT GAAGAAACGA 
GAAAAGAGGC GCTCCAGCAG TAGACAGTTC ATCGATGGCC CTCCTGGGCC TGTGAAGAAA 
ACGTGTTCCA TTGGCTCTAC AATAGACCAG GCAAATGAAT CAATAGTTGC TAAAACAACG 
GTGACTGTCC CCAGTGACGG TGGGCCCATT GAAGCCGTGT CCACTATCGA GACATTGCCG 
TGTTGGACCA GGAGTCGAGG GAAATCAGGA CCTTTACAAC CTGTGAACAG CGACTCTGCT 
CTCAATAGCA GGCCACTGGA GCCAAGAACT GAGACAGACG CTGTGGGCAC ACCCCAGAAT 
AATGGCATGC GCTTACACGA CTTTGTCTCA AAGACGGTTA TTAAGCCTGA ATCTTGTGTC 
CCGTGTGGGA AGCGGATCAA GTTTGGCAAG CTGTCTCTGA AGTGTCGGGA CTGTCGTTTG 
GTCTCTCATC CAGAATGCCG GGACCGATGT CCCCTTCCCT GCATTCCTCC CCTGGTGGGA 
ACCCCCGTGA AGATTGGAGA GGGAATGCTG GCAGATTTTG TGTCGCAGAC TTCCCCAATG 
ATTCCTGCTA TTGTTGTCAG CTGTGTGAAT GAGATTGAGC AGCGAGGCCT GACTGAGGCA 
GGCTTATACA GGATCTCAGG CTGTGACCGG ACAGTAAAGG AGCTGAAAGA GAAATTCCTT 
AAAGTGAAAA CCGTGCCCCT CCTCAGCAAA GTGGACGACA TCCATGCCAT CTGCAGCCTC 
CTGAAAGACT TCCTTCGAAA CCTCAAGGAA CCCCTTCTGA CCTTTTGGCT AAGCAAAGCG 
TTCATGGAGG CAGCAGAGAT AACGGATGAA GACAACAGCA CAGCCGCCAT GTACCAGGCT 
GTCAGTGAGC TGCCCCAGGC CAACAGGGAC ACGCTAGCCT TCCTCATGAT CCACCTCCAG 
AGAGTGGCTC AGAGTCCGAG CACTAAGATG GACGTTGTCA ATCTGGCTAA AATCTTCGGC 
CCTACGATAG TTGCCCACAC TGTGCCCAAT CCAGATCCAG TGACGATGTT CCAGGACATC 
AAACGCCAGC TCAAGGTGGT GGAGCGCCTG CTCTCTCTGC CCCTGGAGTA CTGGAATCAG 
TTCATGATGG TGGAACAAGA GAACATGGAC TGCCACCGCG GCAATGGGAG CTCAACACCA 
CGGACACCAG ACATTAAAGT GAGCTTATTG GGGCCTGTGA CCACTCCTGA ATTCCAGCTT 
GTCAAGACTC CTTCATCAAA TTCCCTGTCA CAGAGATTGT ACAACCTCTC CAAGAGCACA 
CCCAGATTTG GGAACAAGAG CAAGTCTGCC ACCAACTTAG GTCAACAGGG CAAATTTTTC 
CCTGCTCCGT ACCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Racgap1 ( XM_017594892.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450381.1 Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa06699
Clone ID Related Accession (Same CDS sequence)	XM_017594892.1 , XM_006257400.3 , NM_001108112.1
Accession Version	XM_017594892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rac GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CAATGGTGAA TCTGTGGACT CTGTTTGAGC AGCTTGTGCG CCGGCTGGAG 
ATTATCAATG AAGGAAATGA AAGCATTGAA TTCATCCAGG TTGTAAAGGA CTTCGAGGAC 
TTCCGAAAGA AATGTCAAAG AACCAACCAG GAGCTGGAGA AATTCAAAGA TCTGTTGTTG 
AAGGCAGAGA CTGGGCGGAG TGCCCTGGAC GTCAAGCTGA AGCACGCCCG TAACCAAGTG 
GATGTGGAGA TCAAACGGAG GCAACGAGCT GAGGCCGAGT GTGCGAAGCT GGAACAACAG 
ATTCAGCTGA TTCGAGATAT ACTCATGTGT GACACAACTG GCAGCATCCA GCTAAGCGAG 
GAACAAAAAT CAGCTCTGGC TTTTCTCAAC CGAGGCCAAG CATCCAGTGG CAACGCTGGG 
AACAATAGAC TGTCAACGAT TGATGAATCT GGCTCCATTT TATCAGATAT CAGCTTTGAC 
AAGACTGATG AATCACTGGA CTGGGATTCT TCTTTGAAGA ATTTCAAAAT GAAGAAACGA 
GAAAAGAGGC GCTCCAGCAG TAGACAGTTC ATCGATGGCC CTCCTGGGCC TGTGAAGAAA 
ACGTGTTCCA TTGGCTCTAC AATAGACCAG GCAAATGAAT CAATAGTTGC TAAAACAACG 
GTGACTGTCC CCAGTGACGG TGGGCCCATT GAAGCCGTGT CCACTATCGA GACATTGCCG 
TGTTGGACCA GGAGTCGAGG GAAATCAGGA CCTTTACAAC CTGTGAACAG CGACTCTGCT 
CTCAATAGCA GGCCACTGGA GCCAAGAACT GAGACAGACG CTGTGGGCAC ACCCCAGAAT 
AATGGCATGC GCTTACACGA CTTTGTCTCA AAGACGGTTA TTAAGCCTGA ATCTTGTGTC 
CCGTGTGGGA AGCGGATCAA GTTTGGCAAG CTGTCTCTGA AGTGTCGGGA CTGTCGTTTG 
GTCTCTCATC CAGAATGCCG GGACCGATGT CCCCTTCCCT GCATTCCTCC CCTGGTGGGA 
ACCCCCGTGA AGATTGGAGA GGGAATGCTG GCAGATTTTG TGTCGCAGAC TTCCCCAATG 
ATTCCTGCTA TTGTTGTCAG CTGTGTGAAT GAGATTGAGC AGCGAGGCCT GACTGAGGCA 
GGCTTATACA GGATCTCAGG CTGTGACCGG ACAGTAAAGG AGCTGAAAGA GAAATTCCTT 
AAAGTGAAAA CCGTGCCCCT CCTCAGCAAA GTGGACGACA TCCATGCCAT CTGCAGCCTC 
CTGAAAGACT TCCTTCGAAA CCTCAAGGAA CCCCTTCTGA CCTTTTGGCT AAGCAAAGCG 
TTCATGGAGG CAGCAGAGAT AACGGATGAA GACAACAGCA CAGCCGCCAT GTACCAGGCT 
GTCAGTGAGC TGCCCCAGGC CAACAGGGAC ACGCTAGCCT TCCTCATGAT CCACCTCCAG 
AGAGTGGCTC AGAGTCCGAG CACTAAGATG GACGTTGTCA ATCTGGCTAA AATCTTCGGC 
CCTACGATAG TTGCCCACAC TGTGCCCAAT CCAGATCCAG TGACGATGTT CCAGGACATC 
AAACGCCAGC TCAAGGTGGT GGAGCGCCTG CTCTCTCTGC CCCTGGAGTA CTGGAATCAG 
TTCATGATGG TGGAACAAGA GAACATGGAC TGCCACCGCG GCAATGGGAG CTCAACACCA 
CGGACACCAG ACATTAAAGT GAGCTTATTG GGGCCTGTGA CCACTCCTGA ATTCCAGCTT 
GTCAAGACTC CTTCATCAAA TTCCCTGTCA CAGAGATTGT ACAACCTCTC CAAGAGCACA 
CCCAGATTTG GGAACAAGAG CAAGTCTGCC ACCAACTTAG GTCAACAGGG CAAATTTTTC 
CCTGCTCCGT ACCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450381.1
CDS	78..1958
Translation	MDTTMVNLWTLFEQLVRRLEIINEGNESIEFIQVVKDFEDFRKKCQRTNQELEKFKDLLLKAETGRSALDVKLKHARNQVDVEIKRRQRAEAECAKLEQQIQLIRDILMCDTTGSIQLSEEQKSALAFLNRGQASSGNAGNNRLSTIDESGSILSDISFDKTDESLDWDSSLKNFKMKKREKRRSSSRQFIDGPPGPVKKTCSIGSTIDQANESIVAKTTVTVPSDGGPIEAVSTIETLPCWTRSRGKSGPLQPVNSDSALNSRPLEPRTETDAVGTPQNNGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRLVSHPECRDRCPLPCIPPLVGTPVKIGEGMLADFVSQTSPMIPAIVVSCVNEIEQRGLTEAGLYRISGCDRTVKELKEKFLKVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFWLSKAFMEAAEITDEDNSTAAMYQAVSELPQANRDTLAFLMIHLQRVAQSPSTKMDVVNLAKIFGPTIVAHTVPNPDPVTMFQDIKRQLKVVERLLSLPLEYWNQFMMVEQENMDCHRGNGSSTPRTPDIKVSLLGPVTTPEFQLVKTPSSNSLSQRLYNLSKSTPRFGNKSKSATNLGQQGKFFPAPYLK

Target ORF information:

RefSeq Version	XM_017594892.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594892.1

ORF Insert Sequence:

ATGGATACTA CAATGGTGAA TCTGTGGACT CTGTTTGAGC AGCTTGTGCG CCGGCTGGAG 
ATTATCAATG AAGGAAATGA AAGCATTGAA TTCATCCAGG TTGTAAAGGA CTTCGAGGAC 
TTCCGAAAGA AATGTCAAAG AACCAACCAG GAGCTGGAGA AATTCAAAGA TCTGTTGTTG 
AAGGCAGAGA CTGGGCGGAG TGCCCTGGAC GTCAAGCTGA AGCACGCCCG TAACCAAGTG 
GATGTGGAGA TCAAACGGAG GCAACGAGCT GAGGCCGAGT GTGCGAAGCT GGAACAACAG 
ATTCAGCTGA TTCGAGATAT ACTCATGTGT GACACAACTG GCAGCATCCA GCTAAGCGAG 
GAACAAAAAT CAGCTCTGGC TTTTCTCAAC CGAGGCCAAG CATCCAGTGG CAACGCTGGG 
AACAATAGAC TGTCAACGAT TGATGAATCT GGCTCCATTT TATCAGATAT CAGCTTTGAC 
AAGACTGATG AATCACTGGA CTGGGATTCT TCTTTGAAGA ATTTCAAAAT GAAGAAACGA 
GAAAAGAGGC GCTCCAGCAG TAGACAGTTC ATCGATGGCC CTCCTGGGCC TGTGAAGAAA 
ACGTGTTCCA TTGGCTCTAC AATAGACCAG GCAAATGAAT CAATAGTTGC TAAAACAACG 
GTGACTGTCC CCAGTGACGG TGGGCCCATT GAAGCCGTGT CCACTATCGA GACATTGCCG 
TGTTGGACCA GGAGTCGAGG GAAATCAGGA CCTTTACAAC CTGTGAACAG CGACTCTGCT 
CTCAATAGCA GGCCACTGGA GCCAAGAACT GAGACAGACG CTGTGGGCAC ACCCCAGAAT 
AATGGCATGC GCTTACACGA CTTTGTCTCA AAGACGGTTA TTAAGCCTGA ATCTTGTGTC 
CCGTGTGGGA AGCGGATCAA GTTTGGCAAG CTGTCTCTGA AGTGTCGGGA CTGTCGTTTG 
GTCTCTCATC CAGAATGCCG GGACCGATGT CCCCTTCCCT GCATTCCTCC CCTGGTGGGA 
ACCCCCGTGA AGATTGGAGA GGGAATGCTG GCAGATTTTG TGTCGCAGAC TTCCCCAATG 
ATTCCTGCTA TTGTTGTCAG CTGTGTGAAT GAGATTGAGC AGCGAGGCCT GACTGAGGCA 
GGCTTATACA GGATCTCAGG CTGTGACCGG ACAGTAAAGG AGCTGAAAGA GAAATTCCTT 
AAAGTGAAAA CCGTGCCCCT CCTCAGCAAA GTGGACGACA TCCATGCCAT CTGCAGCCTC 
CTGAAAGACT TCCTTCGAAA CCTCAAGGAA CCCCTTCTGA CCTTTTGGCT AAGCAAAGCG 
TTCATGGAGG CAGCAGAGAT AACGGATGAA GACAACAGCA CAGCCGCCAT GTACCAGGCT 
GTCAGTGAGC TGCCCCAGGC CAACAGGGAC ACGCTAGCCT TCCTCATGAT CCACCTCCAG 
AGAGTGGCTC AGAGTCCGAG CACTAAGATG GACGTTGTCA ATCTGGCTAA AATCTTCGGC 
CCTACGATAG TTGCCCACAC TGTGCCCAAT CCAGATCCAG TGACGATGTT CCAGGACATC 
AAACGCCAGC TCAAGGTGGT GGAGCGCCTG CTCTCTCTGC CCCTGGAGTA CTGGAATCAG 
TTCATGATGG TGGAACAAGA GAACATGGAC TGCCACCGCG GCAATGGGAG CTCAACACCA 
CGGACACCAG ACATTAAAGT GAGCTTATTG GGGCCTGTGA CCACTCCTGA ATTCCAGCTT 
GTCAAGACTC CTTCATCAAA TTCCCTGTCA CAGAGATTGT ACAACCTCTC CAAGAGCACA 
CCCAGATTTG GGAACAAGAG CAAGTCTGCC ACCAACTTAG GTCAACAGGG CAAATTTTTC 
CCTGCTCCGT ACCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Racgap1 ( XM_006257400.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257462.1 Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa06699
Clone ID Related Accession (Same CDS sequence)	XM_017594892.1 , XM_006257400.3 , NM_001108112.1
Accession Version	XM_006257400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rac GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CAATGGTGAA TCTGTGGACT CTGTTTGAGC AGCTTGTGCG CCGGCTGGAG 
ATTATCAATG AAGGAAATGA AAGCATTGAA TTCATCCAGG TTGTAAAGGA CTTCGAGGAC 
TTCCGAAAGA AATGTCAAAG AACCAACCAG GAGCTGGAGA AATTCAAAGA TCTGTTGTTG 
AAGGCAGAGA CTGGGCGGAG TGCCCTGGAC GTCAAGCTGA AGCACGCCCG TAACCAAGTG 
GATGTGGAGA TCAAACGGAG GCAACGAGCT GAGGCCGAGT GTGCGAAGCT GGAACAACAG 
ATTCAGCTGA TTCGAGATAT ACTCATGTGT GACACAACTG GCAGCATCCA GCTAAGCGAG 
GAACAAAAAT CAGCTCTGGC TTTTCTCAAC CGAGGCCAAG CATCCAGTGG CAACGCTGGG 
AACAATAGAC TGTCAACGAT TGATGAATCT GGCTCCATTT TATCAGATAT CAGCTTTGAC 
AAGACTGATG AATCACTGGA CTGGGATTCT TCTTTGAAGA ATTTCAAAAT GAAGAAACGA 
GAAAAGAGGC GCTCCAGCAG TAGACAGTTC ATCGATGGCC CTCCTGGGCC TGTGAAGAAA 
ACGTGTTCCA TTGGCTCTAC AATAGACCAG GCAAATGAAT CAATAGTTGC TAAAACAACG 
GTGACTGTCC CCAGTGACGG TGGGCCCATT GAAGCCGTGT CCACTATCGA GACATTGCCG 
TGTTGGACCA GGAGTCGAGG GAAATCAGGA CCTTTACAAC CTGTGAACAG CGACTCTGCT 
CTCAATAGCA GGCCACTGGA GCCAAGAACT GAGACAGACG CTGTGGGCAC ACCCCAGAAT 
AATGGCATGC GCTTACACGA CTTTGTCTCA AAGACGGTTA TTAAGCCTGA ATCTTGTGTC 
CCGTGTGGGA AGCGGATCAA GTTTGGCAAG CTGTCTCTGA AGTGTCGGGA CTGTCGTTTG 
GTCTCTCATC CAGAATGCCG GGACCGATGT CCCCTTCCCT GCATTCCTCC CCTGGTGGGA 
ACCCCCGTGA AGATTGGAGA GGGAATGCTG GCAGATTTTG TGTCGCAGAC TTCCCCAATG 
ATTCCTGCTA TTGTTGTCAG CTGTGTGAAT GAGATTGAGC AGCGAGGCCT GACTGAGGCA 
GGCTTATACA GGATCTCAGG CTGTGACCGG ACAGTAAAGG AGCTGAAAGA GAAATTCCTT 
AAAGTGAAAA CCGTGCCCCT CCTCAGCAAA GTGGACGACA TCCATGCCAT CTGCAGCCTC 
CTGAAAGACT TCCTTCGAAA CCTCAAGGAA CCCCTTCTGA CCTTTTGGCT AAGCAAAGCG 
TTCATGGAGG CAGCAGAGAT AACGGATGAA GACAACAGCA CAGCCGCCAT GTACCAGGCT 
GTCAGTGAGC TGCCCCAGGC CAACAGGGAC ACGCTAGCCT TCCTCATGAT CCACCTCCAG 
AGAGTGGCTC AGAGTCCGAG CACTAAGATG GACGTTGTCA ATCTGGCTAA AATCTTCGGC 
CCTACGATAG TTGCCCACAC TGTGCCCAAT CCAGATCCAG TGACGATGTT CCAGGACATC 
AAACGCCAGC TCAAGGTGGT GGAGCGCCTG CTCTCTCTGC CCCTGGAGTA CTGGAATCAG 
TTCATGATGG TGGAACAAGA GAACATGGAC TGCCACCGCG GCAATGGGAG CTCAACACCA 
CGGACACCAG ACATTAAAGT GAGCTTATTG GGGCCTGTGA CCACTCCTGA ATTCCAGCTT 
GTCAAGACTC CTTCATCAAA TTCCCTGTCA CAGAGATTGT ACAACCTCTC CAAGAGCACA 
CCCAGATTTG GGAACAAGAG CAAGTCTGCC ACCAACTTAG GTCAACAGGG CAAATTTTTC 
CCTGCTCCGT ACCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257462.1
CDS	130..2010
Translation	MDTTMVNLWTLFEQLVRRLEIINEGNESIEFIQVVKDFEDFRKKCQRTNQELEKFKDLLLKAETGRSALDVKLKHARNQVDVEIKRRQRAEAECAKLEQQIQLIRDILMCDTTGSIQLSEEQKSALAFLNRGQASSGNAGNNRLSTIDESGSILSDISFDKTDESLDWDSSLKNFKMKKREKRRSSSRQFIDGPPGPVKKTCSIGSTIDQANESIVAKTTVTVPSDGGPIEAVSTIETLPCWTRSRGKSGPLQPVNSDSALNSRPLEPRTETDAVGTPQNNGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRLVSHPECRDRCPLPCIPPLVGTPVKIGEGMLADFVSQTSPMIPAIVVSCVNEIEQRGLTEAGLYRISGCDRTVKELKEKFLKVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFWLSKAFMEAAEITDEDNSTAAMYQAVSELPQANRDTLAFLMIHLQRVAQSPSTKMDVVNLAKIFGPTIVAHTVPNPDPVTMFQDIKRQLKVVERLLSLPLEYWNQFMMVEQENMDCHRGNGSSTPRTPDIKVSLLGPVTTPEFQLVKTPSSNSLSQRLYNLSKSTPRFGNKSKSATNLGQQGKFFPAPYLK

Target ORF information:

RefSeq Version	XM_006257400.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rac GTPase-activating protein 1 (Racgap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257400.3

ORF Insert Sequence:

ATGGATACTA CAATGGTGAA TCTGTGGACT CTGTTTGAGC AGCTTGTGCG CCGGCTGGAG 
ATTATCAATG AAGGAAATGA AAGCATTGAA TTCATCCAGG TTGTAAAGGA CTTCGAGGAC 
TTCCGAAAGA AATGTCAAAG AACCAACCAG GAGCTGGAGA AATTCAAAGA TCTGTTGTTG 
AAGGCAGAGA CTGGGCGGAG TGCCCTGGAC GTCAAGCTGA AGCACGCCCG TAACCAAGTG 
GATGTGGAGA TCAAACGGAG GCAACGAGCT GAGGCCGAGT GTGCGAAGCT GGAACAACAG 
ATTCAGCTGA TTCGAGATAT ACTCATGTGT GACACAACTG GCAGCATCCA GCTAAGCGAG 
GAACAAAAAT CAGCTCTGGC TTTTCTCAAC CGAGGCCAAG CATCCAGTGG CAACGCTGGG 
AACAATAGAC TGTCAACGAT TGATGAATCT GGCTCCATTT TATCAGATAT CAGCTTTGAC 
AAGACTGATG AATCACTGGA CTGGGATTCT TCTTTGAAGA ATTTCAAAAT GAAGAAACGA 
GAAAAGAGGC GCTCCAGCAG TAGACAGTTC ATCGATGGCC CTCCTGGGCC TGTGAAGAAA 
ACGTGTTCCA TTGGCTCTAC AATAGACCAG GCAAATGAAT CAATAGTTGC TAAAACAACG 
GTGACTGTCC CCAGTGACGG TGGGCCCATT GAAGCCGTGT CCACTATCGA GACATTGCCG 
TGTTGGACCA GGAGTCGAGG GAAATCAGGA CCTTTACAAC CTGTGAACAG CGACTCTGCT 
CTCAATAGCA GGCCACTGGA GCCAAGAACT GAGACAGACG CTGTGGGCAC ACCCCAGAAT 
AATGGCATGC GCTTACACGA CTTTGTCTCA AAGACGGTTA TTAAGCCTGA ATCTTGTGTC 
CCGTGTGGGA AGCGGATCAA GTTTGGCAAG CTGTCTCTGA AGTGTCGGGA CTGTCGTTTG 
GTCTCTCATC CAGAATGCCG GGACCGATGT CCCCTTCCCT GCATTCCTCC CCTGGTGGGA 
ACCCCCGTGA AGATTGGAGA GGGAATGCTG GCAGATTTTG TGTCGCAGAC TTCCCCAATG 
ATTCCTGCTA TTGTTGTCAG CTGTGTGAAT GAGATTGAGC AGCGAGGCCT GACTGAGGCA 
GGCTTATACA GGATCTCAGG CTGTGACCGG ACAGTAAAGG AGCTGAAAGA GAAATTCCTT 
AAAGTGAAAA CCGTGCCCCT CCTCAGCAAA GTGGACGACA TCCATGCCAT CTGCAGCCTC 
CTGAAAGACT TCCTTCGAAA CCTCAAGGAA CCCCTTCTGA CCTTTTGGCT AAGCAAAGCG 
TTCATGGAGG CAGCAGAGAT AACGGATGAA GACAACAGCA CAGCCGCCAT GTACCAGGCT 
GTCAGTGAGC TGCCCCAGGC CAACAGGGAC ACGCTAGCCT TCCTCATGAT CCACCTCCAG 
AGAGTGGCTC AGAGTCCGAG CACTAAGATG GACGTTGTCA ATCTGGCTAA AATCTTCGGC 
CCTACGATAG TTGCCCACAC TGTGCCCAAT CCAGATCCAG TGACGATGTT CCAGGACATC 
AAACGCCAGC TCAAGGTGGT GGAGCGCCTG CTCTCTCTGC CCCTGGAGTA CTGGAATCAG 
TTCATGATGG TGGAACAAGA GAACATGGAC TGCCACCGCG GCAATGGGAG CTCAACACCA 
CGGACACCAG ACATTAAAGT GAGCTTATTG GGGCCTGTGA CCACTCCTGA ATTCCAGCTT 
GTCAAGACTC CTTCATCAAA TTCCCTGTCA CAGAGATTGT ACAACCTCTC CAAGAGCACA 
CCCAGATTTG GGAACAAGAG CAAGTCTGCC ACCAACTTAG GTCAACAGGG CAAATTTTTC 
CCTGCTCCGT ACCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis.
Nature492(7428)276-9(2012 Dec)
Lekomtsev S,Su KC,Pye VE,Blight K,Sundaramoorthy S,Takaki T,Collinson LM,Cherepanov P,Divecha N,Petronczki M

Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells.
PLoS biology7(5)e1000111(2009 May)
Burkard ME,Maciejowski J,Rodriguez-Bravo V,Repka M,Lowery DM,Clauser KR,Zhang C,Shokat KM,Carr SA,Yaffe MB,Jallepalli PV

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis.
The EMBO journal27(13)1791-803(2008 Jul)
Simon GC,Schonteich E,Wu CC,Piekny A,Ekiert D,Yu X,Gould GW,Glotzer M,Prekeris R

Dissecting the role of Rho-mediated signaling in contractile ring formation.
Molecular biology of the cell17(1)43-55(2006 Jan)
Kamijo K,Ohara N,Abe M,Uchimura T,Hosoya H,Lee JS,Miki T

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Racgap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

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