Hif1an cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hif1an
Entrez Gene ID	309434
Full Name	hypoxia-inducible factor 1, alpha subunit inhibitor
General protein information	Preferred Names hypoxia-inducible factor 1, alpha subunit inhibitor Names hypoxia-inducible factor 1-alpha inhibitor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113749.1	NP_001107221.1	hypoxia-inducible factor 1-alpha inhibitor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hif1an	NP_795932.2
Xenopus tropicalis (tropical clawed frog)	hif1an	NP_988915.1
Pan troglodytes (chimpanzee)	HIF1AN	XP_521585.2
Rattus norvegicus (Norway rat)	Hif1an	NP_001107221.1
Gallus gallus (chicken)	HIF1AN	XP_426507.2
Danio rerio (zebrafish)	hif1an	NP_958904.1
Homo sapiens (human)	HIF1AN	NP_060372.2
	HIF1AN	XP_001108283.2
Canis lupus familiaris (dog)	HIF1AN	XP_851531.2
Bos taurus (cattle)	HIF1AN	NP_001076912.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of the miR-31/FIH1 pathway in TGF-?-induced liver fibrosis.
Hu J, Chen C, Liu Q, Liu B, Song C, Zhu S, Wu C, Liu S, Yu H, Yao D, Kang J, Zhu L
Clinical science (London, England : 1979)129(4)305-17(2015 Aug)

Cardioprotective effect of miR-214 in myocardial ischemic postconditioning by down-regulation of hypoxia inducible factor 1, alpha subunit inhibitor.
Wan DY, Zhang Z, Yang HH
Cellular and molecular biology (Noisy-le-Grand, France)61(2)1-6(2015 May)

FIH-1-Mint3 axis does not control HIF-1 transcriptional activity in nucleus pulposus cells.
Hirose Y, Johnson ZI, Schoepflin ZR, Markova DZ, Chiba K, Toyama Y, Shapiro IM, Risbud MV
The Journal of biological chemistry289(30)20594-605(2014 Jul)

HIF1? and HIF2?: sibling rivalry in hypoxic tumour growth and progression.
Keith B, Johnson RS, Simon MC
Nature reviews. Cancer12(1)9-22(2011 Dec)

Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains.
Yang M, Chowdhury R, Ge W, Hamed RB, McDonough MA, Claridge TD, Kessler BM, Cockman ME, Ratcliffe PJ, Schofield CJ
The FEBS journal278(7)1086-97(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-31/FIH1 pathway associates with liver fibrosis, perhaps by participation in the TGF-beta/Smad3 signalling of hepatic stellate cells.
Title: The role of the miR-31/FIH1 pathway in TGF-?-induced liver fibrosis.

The expression of HIF1AN remarkably decreased in the ischemia postconditioning group. This indicated that the downregulation of HIF1AN could protect cardiac cells.
Title: Cardioprotective effect of miR-214 in myocardial ischemic postconditioning by down-regulation of hypoxia inducible factor 1, alpha subunit inhibitor.

FIH-1 activity does not represent a major mechanism by which NP cells control HIF-1-dependent transcription, a testament to their adaptation to a unique hypoxic niche.
Title: FIH-1-Mint3 axis does not control HIF-1 transcriptional activity in nucleus pulposus cells.

FIH1 appears to be a suppressor of oxygen-dependent genes in the kidney, operating through HIF-dependent and -independent mechanisms.
Title: Factor inhibiting HIF limits the expression of hypoxia-inducible genes in podocytes and distal tubular cells.

The following Hif1an gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hif1an cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01019	NM_001113749.1 Latest version!	Rattus norvegicus hypoxia inducible factor 1 subunit alpha inhibitor (Hif1an), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hif1an ( NM_001113749.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001107221.1 Rattus norvegicus hypoxia inducible factor 1 subunit alpha inhibitor (Hif1an), mRNA.

Price & Availability↑

CloneID	ORa01019
Clone ID Related Accession (Same CDS sequence)	NM_001113749.1
Accession Version	NM_001113749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-02
Organism	Rattus norvegicus(Norway rat)
Product	hypoxia-inducible factor 1-alpha inhibitor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC158810.1. On or before Sep 7, 2008 this sequence version replaced XM_001062399.1, XM_219961.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158810.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGA CGGCAGCCGA GGTTGCGGCC TCTGCCTCTG GAGAGGCCCG GGAAGAAGCT 
GAAGCTCCGG GCCCGGCCTG GGATGAGTCC CAGCTACGAA GTTACAGCTT TCCGACCAGG 
CCCATCCCAC GTTTGAGCCA GAGCGACCCT CGGGCGGAAG AGCTCATCGA AAATGAGGAG 
CCCGTGGTGC TGACCGACAC AAACCTTGTG TATCCTGCTC TGAAGTGGGA CCTCGAATAC 
CTGCAAGAGA ATATTGGCAA CGGAGATTTC TCTGTGTACA GTGCCAGCAC CCATAAGTTC 
TTATACTATG ATGAGAAGAA GATGGCCAAT TTCCAGAACT TTAAGCCAAG GTCCAACAGG 
GAGGAAATTA AATTTCATGA GTTCGTTGAG AAACTACAAG CTATCCAGCA GCGCGGGGGA 
GAAGAGCGGT TGTATCTGCA GCAAACACTC AATGACACCG TGGGTAGAAA GATTGTCATG 
GACTTCTTGG GTTTTAACTG GAACTGGATT AATAAACAAC AGGGGAAACG TGGCTGGGGG 
CAGCTGACCT CTAACCTGTT GCTCATCGGC ATGGAAGGGA ATGTGACACC TGCTCACTAT 
GATGAGCAAC AGAACTTTTT TGCCCAGATA AAAGGCCATA AGAGATGCAT CTTATTTCCT 
CCGGATCAGT TCGAGTGCCT CTACCCATAC CCTGTCCATC ACCCCTGTGA CAGGCAGAGC 
CAGGTGGACT TTGACAATCC TGACTATGAG CGCTTCCCCA ACTTCCGAAA TGTGGTTGGC 
TATGAAACAG TGGTTGGCCC TGGTGACGTT CTTTACATCC CAATGTACTG GTGGCATCAC 
ATAGAGTCGT TACTAAATGG GGGAATTACC ATCACTGTGA ACTTCTGGTA TAAGGGGGCT 
CCCACCCCTA AGAGAATTGA ATATCCTCTC AAAGCTCATC AGAAAGTAGC CATCATGAGA 
AACATTGAGA AGATGCTTGG AGAGGCCTTG GGGAACCCAC AAGAGGTGGG GCCTTTGTTG 
AACACCATGA TTAAAGGCCG CTACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107221.1
CDS	12..1061
Translation	MAATAAEVAASASGEAREEAEAPGPAWDESQLRSYSFPTRPIPRLSQSDPRAEELIENEEPVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKFLYYDEKKMANFQNFKPRSNREEIKFHEFVEKLQAIQQRGGEERLYLQQTLNDTVGRKIVMDFLGFNWNWINKQQGKRGWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQIKGHKRCILFPPDQFECLYPYPVHHPCDRQSQVDFDNPDYERFPNFRNVVGYETVVGPGDVLYIPMYWWHHIESLLNGGITITVNFWYKGAPTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQEVGPLLNTMIKGRYN

Target ORF information:

RefSeq Version	NM_001113749.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hypoxia inducible factor 1 subunit alpha inhibitor (Hif1an), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113749.1

ORF Insert Sequence:

ATGGCGGCGA CGGCAGCCGA GGTTGCGGCC TCTGCCTCTG GAGAGGCCCG GGAAGAAGCT 
GAAGCTCCGG GCCCGGCCTG GGATGAGTCC CAGCTACGAA GTTACAGCTT TCCGACCAGG 
CCCATCCCAC GTTTGAGCCA GAGCGACCCT CGGGCGGAAG AGCTCATCGA AAATGAGGAG 
CCCGTGGTGC TGACCGACAC AAACCTTGTG TATCCTGCTC TGAAGTGGGA CCTCGAATAC 
CTGCAAGAGA ATATTGGCAA CGGAGATTTC TCTGTGTACA GTGCCAGCAC CCATAAGTTC 
TTATACTATG ATGAGAAGAA GATGGCCAAT TTCCAGAACT TTAAGCCAAG GTCCAACAGG 
GAGGAAATTA AATTTCATGA GTTCGTTGAG AAACTACAAG CTATCCAGCA GCGCGGGGGA 
GAAGAGCGGT TGTATCTGCA GCAAACACTC AATGACACCG TGGGTAGAAA GATTGTCATG 
GACTTCTTGG GTTTTAACTG GAACTGGATT AATAAACAAC AGGGGAAACG TGGCTGGGGG 
CAGCTGACCT CTAACCTGTT GCTCATCGGC ATGGAAGGGA ATGTGACACC TGCTCACTAT 
GATGAGCAAC AGAACTTTTT TGCCCAGATA AAAGGCCATA AGAGATGCAT CTTATTTCCT 
CCGGATCAGT TCGAGTGCCT CTACCCATAC CCTGTCCATC ACCCCTGTGA CAGGCAGAGC 
CAGGTGGACT TTGACAATCC TGACTATGAG CGCTTCCCCA ACTTCCGAAA TGTGGTTGGC 
TATGAAACAG TGGTTGGCCC TGGTGACGTT CTTTACATCC CAATGTACTG GTGGCATCAC 
ATAGAGTCGT TACTAAATGG GGGAATTACC ATCACTGTGA ACTTCTGGTA TAAGGGGGCT 
CCCACCCCTA AGAGAATTGA ATATCCTCTC AAAGCTCATC AGAAAGTAGC CATCATGAGA 
AACATTGAGA AGATGCTTGG AGAGGCCTTG GGGAACCCAC AAGAGGTGGG GCCTTTGTTG 
AACACCATGA TTAAAGGCCG CTACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The role of the miR-31/FIH1 pathway in TGF-?-induced liver fibrosis.
Clinical science (London, England : 1979)129(4)305-17(2015 Aug)
Hu J,Chen C,Liu Q,Liu B,Song C,Zhu S,Wu C,Liu S,Yu H,Yao D,Kang J,Zhu L

Cardioprotective effect of miR-214 in myocardial ischemic postconditioning by down-regulation of hypoxia inducible factor 1, alpha subunit inhibitor.
Cellular and molecular biology (Noisy-le-Grand, France)61(2)1-6(2015 May)
Wan DY,Zhang Z,Yang HH

FIH-1-Mint3 axis does not control HIF-1 transcriptional activity in nucleus pulposus cells.
The Journal of biological chemistry289(30)20594-605(2014 Jul)
Hirose Y,Johnson ZI,Schoepflin ZR,Markova DZ,Chiba K,Toyama Y,Shapiro IM,Risbud MV

HIF1? and HIF2?: sibling rivalry in hypoxic tumour growth and progression.
Nature reviews. Cancer12(1)9-22(2011 Dec)
Keith B,Johnson RS,Simon MC

Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains.
The FEBS journal278(7)1086-97(2011 Apr)
Yang M,Chowdhury R,Ge W,Hamed RB,McDonough MA,Claridge TD,Kessler BM,Cockman ME,Ratcliffe PJ,Schofield CJ