Dagla cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dagla
Entrez Gene ID	309207
Full Name	diacylglycerol lipase, alpha
Synonyms	Nsddr
General protein information	Preferred Names diacylglycerol lipase, alpha Names sn1-specific diacylglycerol lipase alpha DGL-alpha neural stem cell-derived dendrite regulator
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005886.1	NP_001005886.1	sn1-specific diacylglycerol lipase alpha
XM_017589261.1	XP_017444750.1	sn1-specific diacylglycerol lipase alpha isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dagla	XP_697873.5
Homo sapiens (human)	DAGLA	NP_006124.1
Canis lupus familiaris (dog)	DAGLA	XP_005631813.1
Mus musculus (house mouse)	Dagla	NP_932782.2
Gallus gallus (chicken)	DAGLA	XP_423696.3
Xenopus tropicalis (tropical clawed frog)	dagla	XP_004913466.1
Rattus norvegicus (Norway rat)	Dagla	NP_001005886.1
Pan troglodytes (chimpanzee)	DAGLA	XP_508479.4
Bos taurus (cattle)	DAGLA	NP_001179512.1

Gene Ontology
Bibliography

Related articles in PubMed

Uncoupling of the endocannabinoid signalling complex in a mouse model of fragile X syndrome.
Jung KM, Sepers M, Henstridge CM, Lassalle O, Neuhofer D, Martin H, Ginger M, Frick A, DiPatrizio NV, Mackie K, Katona I, Piomelli D, Manzoni OJ
Nature communications31080(2012)

Distribution of diacylglycerol lipase alpha, an endocannabinoid synthesizing enzyme, in the rat forebrain.
Su?rez J, Ort?z O, Puente N, Berm?dez-Silva FJ, Blanco E, Fern?ndez-Llebrez P, Grandes P, de Fonseca FR, Moratalla R
Neuroscience192112-31(2011 Sep)

Endocannabinoids generated by Ca2+ or by metabotropic glutamate receptors appear to arise from different pools of diacylglycerol lipase.
Zhang L, Wang M, Bisogno T, Di Marzo V, Alger BE
PloS one6(1)e16305(2011 Jan)

Differential subcellular recruitment of monoacylglycerol lipase generates spatial specificity of 2-arachidonoyl glycerol signaling during axonal pathfinding.
Keimpema E, Barabas K, Morozov YM, Tortoriello G, Torii M, Cameron G, Yanagawa Y, Watanabe M, Mackie K, Harkany T
The Journal of neuroscience : the official journal of the Society for Neuroscience30(42)13992-4007(2010 Oct)

Early maternal deprivation induces changes on the expression of 2-AG biosynthesis and degradation enzymes in neonatal rat hippocampus.
Su?rez J, Rivera P, Llorente R, Romero-Zerbo SY, Berm?dez-Silva FJ, de Fonseca FR, Viveros MP
Brain research1349162-73(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we showed here an integrated description of the distribution of 1,2-diacylglycerol lipase alpha in the rat forebrain
Title: Distribution of diacylglycerol lipase alpha, an endocannabinoid synthesizing enzyme, in the rat forebrain.

pharmacological inhibitors of DGLalpha have quantitatively different effects on eCB-mediated responses triggered by different stimuli, suggesting that functional, and perhaps physical, distinctions among pools of DAGLalpha exist.
Title: Endocannabinoids generated by Ca2+ or by metabotropic glutamate receptors appear to arise from different pools of diacylglycerol lipase.

Maternal deprivation induced a significant increase in DAGLalpha immunoreactivity in the rat both males and females.
Title: Early maternal deprivation induces changes on the expression of 2-AG biosynthesis and degradation enzymes in neonatal rat hippocampus.

Data show that L- and N-type calcium channel current, but not M-current inhibition by M1 muscarinic receptors requires DAG lipase activity.
Title: L- and N-current but not M-current inhibition by M1 muscarinic receptors requires DAG lipase activity.

The following Dagla gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dagla cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10787	NM_001005886.1 Latest version!	Rattus norvegicus diacylglycerol lipase, alpha (Dagla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa10787	XM_017589261.1 Latest version!	Rattus norvegicus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dagla ( NM_001005886.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001005886.1 Rattus norvegicus diacylglycerol lipase, alpha (Dagla), mRNA.

Price & Availability↑

CloneID	ORa10787
Clone ID Related Accession (Same CDS sequence)	NM_001005886.1 , XM_017589261.1
Accession Version	NM_001005886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY275375.1. On Oct 22, 2004 this sequence version replaced XM_219575.2. ##Evidence-Data-START## Transcript exon combination :: AY275375.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTGCTG 
CCTGCTATCT TCCTCTTCCT GCTGCACACC ACCTGGTTTG TGATCCTGTC TGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCTGAGA TGGCTATCAT TTGGCTGAGC 
ATGCGTGGGG GTATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TTTTGGTGAT CGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTTACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACTCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT CAGCGTCTGC ATCACTGTCC TCTGTGTCTT TGACCCCACT 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAACCT GCGGACTTAC 
AACCTGCGGC ATCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTATTCAGA AATTGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATAGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GGCAGCGGCA GCGAGCCAAG CGCAATGCGG TGCTGGATGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTC 
GCTGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGCCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGTTG TCTCTGCCCT GCACGCCCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAT GCAATTGCCA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA CATCGTCTAT ACCTCCTGTC ATGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGAGATG CTGAGCGTCT CCCTGTGGAG 
GGACACCGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATT 
CTCTCCTTCC TCCTGCGCCC TCAGTACCCG ACCCTCAAGT GCTTCGCCTA CTCCCCTCCA 
GGAGGCCTGC TGAGTGAGGA TGCCATGGAG TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATCGGC CTTTCCCAGC TCGAAGGTTT CCGCCGGCAG 
CTTCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCTGAG GATCAGGTGG AAGTGACTGC CTTGGCCAGC 
ACACGGCTCT GGACACACCC CAGTGATCTG ACCATCGCCC TTTCAGCCAG CACCCCACTC 
TACCCACCTG GCCGTATCAT CCATGTGGTC CATAACCACC CTGCTGAACA GTGCTGCTGC 
TGTGAACAGG AAGAGCCCAC ATACTTTGCC ATCTGGGGTG ACAACAAAGC CTTTAATGAG 
GTGATCATCT CACCAGCCAT GCTCCATGAG CACCTGCCCT ACGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAGGGA AAGACAGCGC TGCTCTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACTGAGGT AGACCTCACT CCTGAGCTCA TCTTCCAGCA ACAGCCGCTG 
CCCACGGGGC CACCTCTGCC CACTGGGCTG GCTCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TAGAGGGCTT CTCTGAAGGA 
CGGCTGCTGT CCCCAGTGGC TGCTGCCTCA GCAGCCCGGC AAGACCCTGT AGAGCTGTTG 
CTGCTGTCCA CCCAGGAACG ACTGGCCGCG GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACTATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TTGACTCACG CCGTTCCTCA 
GGCTTCCGCA GCATCAGAGG CTCACCCAGC CTCCATGCAG TACTGGAGCG TGATGAGGGC 
CACCTGTTTT ACATCGACCC AGCCATTCCA GAGGAGAACC CATCCCTCAG CTCCCGCACA 
GAGCTGCTGG CAGCTGACAG TCTGTCCAAG CACTCGCAGG ACACCCAACC CCTGGAGGCA 
GCACTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAGCCAA TGACGAGGAG 
GAGGCAGCCG GTGGCAGTGA GGGTGGTGGG GTGGCCCCAC GAGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGATTC GCCCAGCCCT CAGGTGCTAG AATTCGCTGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTGTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACAGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAATCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCCGAAC CCTCCTCTGG CATCTCACTC TCACCCTCCT TCCCACTCAG CTCCTCAGGG 
GAGCTCATGG ACCTGACACC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCATGGG GCCAGCCCTA CCAAGCAGGA TGACCTGGTC 
ATCTCAGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005886.1
CDS	94..3228
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTALASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAANDEEEAAGGSEGGGVAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	NM_001005886.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol lipase, alpha (Dagla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005886.1

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTGCTG 
CCTGCTATCT TCCTCTTCCT GCTGCACACC ACCTGGTTTG TGATCCTGTC TGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCTGAGA TGGCTATCAT TTGGCTGAGC 
ATGCGTGGGG GTATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TTTTGGTGAT CGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTTACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACTCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT CAGCGTCTGC ATCACTGTCC TCTGTGTCTT TGACCCCACT 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAACCT GCGGACTTAC 
AACCTGCGGC ATCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTATTCAGA AATTGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATAGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GGCAGCGGCA GCGAGCCAAG CGCAATGCGG TGCTGGATGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTC 
GCTGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGCCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGTTG TCTCTGCCCT GCACGCCCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAT GCAATTGCCA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA CATCGTCTAT ACCTCCTGTC ATGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGAGATG CTGAGCGTCT CCCTGTGGAG 
GGACACCGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATT 
CTCTCCTTCC TCCTGCGCCC TCAGTACCCG ACCCTCAAGT GCTTCGCCTA CTCCCCTCCA 
GGAGGCCTGC TGAGTGAGGA TGCCATGGAG TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATCGGC CTTTCCCAGC TCGAAGGTTT CCGCCGGCAG 
CTTCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCTGAG GATCAGGTGG AAGTGACTGC CTTGGCCAGC 
ACACGGCTCT GGACACACCC CAGTGATCTG ACCATCGCCC TTTCAGCCAG CACCCCACTC 
TACCCACCTG GCCGTATCAT CCATGTGGTC CATAACCACC CTGCTGAACA GTGCTGCTGC 
TGTGAACAGG AAGAGCCCAC ATACTTTGCC ATCTGGGGTG ACAACAAAGC CTTTAATGAG 
GTGATCATCT CACCAGCCAT GCTCCATGAG CACCTGCCCT ACGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAGGGA AAGACAGCGC TGCTCTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACTGAGGT AGACCTCACT CCTGAGCTCA TCTTCCAGCA ACAGCCGCTG 
CCCACGGGGC CACCTCTGCC CACTGGGCTG GCTCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TAGAGGGCTT CTCTGAAGGA 
CGGCTGCTGT CCCCAGTGGC TGCTGCCTCA GCAGCCCGGC AAGACCCTGT AGAGCTGTTG 
CTGCTGTCCA CCCAGGAACG ACTGGCCGCG GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACTATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TTGACTCACG CCGTTCCTCA 
GGCTTCCGCA GCATCAGAGG CTCACCCAGC CTCCATGCAG TACTGGAGCG TGATGAGGGC 
CACCTGTTTT ACATCGACCC AGCCATTCCA GAGGAGAACC CATCCCTCAG CTCCCGCACA 
GAGCTGCTGG CAGCTGACAG TCTGTCCAAG CACTCGCAGG ACACCCAACC CCTGGAGGCA 
GCACTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAGCCAA TGACGAGGAG 
GAGGCAGCCG GTGGCAGTGA GGGTGGTGGG GTGGCCCCAC GAGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGATTC GCCCAGCCCT CAGGTGCTAG AATTCGCTGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTGTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACAGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAATCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCCGAAC CCTCCTCTGG CATCTCACTC TCACCCTCCT TCCCACTCAG CTCCTCAGGG 
GAGCTCATGG ACCTGACACC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCATGGG GCCAGCCCTA CCAAGCAGGA TGACCTGGTC 
ATCTCAGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dagla ( XM_017589261.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444750.1 Rattus norvegicus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10787
Clone ID Related Accession (Same CDS sequence)	NM_001005886.1 , XM_017589261.1
Accession Version	XM_017589261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTGCTG 
CCTGCTATCT TCCTCTTCCT GCTGCACACC ACCTGGTTTG TGATCCTGTC TGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCTGAGA TGGCTATCAT TTGGCTGAGC 
ATGCGTGGGG GTATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TTTTGGTGAT CGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTTACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACTCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT CAGCGTCTGC ATCACTGTCC TCTGTGTCTT TGACCCCACT 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAACCT GCGGACTTAC 
AACCTGCGGC ATCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTATTCAGA AATTGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATAGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GGCAGCGGCA GCGAGCCAAG CGCAATGCGG TGCTGGATGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTC 
GCTGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGCCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGTTG TCTCTGCCCT GCACGCCCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAT GCAATTGCCA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA CATCGTCTAT ACCTCCTGTC ATGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGAGATG CTGAGCGTCT CCCTGTGGAG 
GGACACCGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATT 
CTCTCCTTCC TCCTGCGCCC TCAGTACCCG ACCCTCAAGT GCTTCGCCTA CTCCCCTCCA 
GGAGGCCTGC TGAGTGAGGA TGCCATGGAG TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATCGGC CTTTCCCAGC TCGAAGGTTT CCGCCGGCAG 
CTTCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCTGAG GATCAGGTGG AAGTGACTGC CTTGGCCAGC 
ACACGGCTCT GGACACACCC CAGTGATCTG ACCATCGCCC TTTCAGCCAG CACCCCACTC 
TACCCACCTG GCCGTATCAT CCATGTGGTC CATAACCACC CTGCTGAACA GTGCTGCTGC 
TGTGAACAGG AAGAGCCCAC ATACTTTGCC ATCTGGGGTG ACAACAAAGC CTTTAATGAG 
GTGATCATCT CACCAGCCAT GCTCCATGAG CACCTGCCCT ACGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAGGGA AAGACAGCGC TGCTCTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACTGAGGT AGACCTCACT CCTGAGCTCA TCTTCCAGCA ACAGCCGCTG 
CCCACGGGGC CACCTCTGCC CACTGGGCTG GCTCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TAGAGGGCTT CTCTGAAGGA 
CGGCTGCTGT CCCCAGTGGC TGCTGCCTCA GCAGCCCGGC AAGACCCTGT AGAGCTGTTG 
CTGCTGTCCA CCCAGGAACG ACTGGCCGCG GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACTATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TTGACTCACG CCGTTCCTCA 
GGCTTCCGCA GCATCAGAGG CTCACCCAGC CTCCATGCAG TACTGGAGCG TGATGAGGGC 
CACCTGTTTT ACATCGACCC AGCCATTCCA GAGGAGAACC CATCCCTCAG CTCCCGCACA 
GAGCTGCTGG CAGCTGACAG TCTGTCCAAG CACTCGCAGG ACACCCAACC CCTGGAGGCA 
GCACTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAGCCAA TGACGAGGAG 
GAGGCAGCCG GTGGCAGTGA GGGTGGTGGG GTGGCCCCAC GAGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGATTC GCCCAGCCCT CAGGTGCTAG AATTCGCTGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTGTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACAGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAATCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCCGAAC CCTCCTCTGG CATCTCACTC TCACCCTCCT TCCCACTCAG CTCCTCAGGG 
GAGCTCATGG ACCTGACACC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCATGGG GCCAGCCCTA CCAAGCAGGA TGACCTGGTC 
ATCTCAGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444750.1
CDS	266..3400
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTALASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAANDEEEAAGGSEGGGVAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_017589261.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589261.1

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTGCTG 
CCTGCTATCT TCCTCTTCCT GCTGCACACC ACCTGGTTTG TGATCCTGTC TGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCTGAGA TGGCTATCAT TTGGCTGAGC 
ATGCGTGGGG GTATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TTTTGGTGAT CGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTTACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACTCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT CAGCGTCTGC ATCACTGTCC TCTGTGTCTT TGACCCCACT 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAACCT GCGGACTTAC 
AACCTGCGGC ATCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTATTCAGA AATTGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATAGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GGCAGCGGCA GCGAGCCAAG CGCAATGCGG TGCTGGATGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTC 
GCTGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGCCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGTTG TCTCTGCCCT GCACGCCCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAT GCAATTGCCA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA CATCGTCTAT ACCTCCTGTC ATGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGAGATG CTGAGCGTCT CCCTGTGGAG 
GGACACCGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATT 
CTCTCCTTCC TCCTGCGCCC TCAGTACCCG ACCCTCAAGT GCTTCGCCTA CTCCCCTCCA 
GGAGGCCTGC TGAGTGAGGA TGCCATGGAG TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATCGGC CTTTCCCAGC TCGAAGGTTT CCGCCGGCAG 
CTTCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCTGAG GATCAGGTGG AAGTGACTGC CTTGGCCAGC 
ACACGGCTCT GGACACACCC CAGTGATCTG ACCATCGCCC TTTCAGCCAG CACCCCACTC 
TACCCACCTG GCCGTATCAT CCATGTGGTC CATAACCACC CTGCTGAACA GTGCTGCTGC 
TGTGAACAGG AAGAGCCCAC ATACTTTGCC ATCTGGGGTG ACAACAAAGC CTTTAATGAG 
GTGATCATCT CACCAGCCAT GCTCCATGAG CACCTGCCCT ACGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAGGGA AAGACAGCGC TGCTCTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACTGAGGT AGACCTCACT CCTGAGCTCA TCTTCCAGCA ACAGCCGCTG 
CCCACGGGGC CACCTCTGCC CACTGGGCTG GCTCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TAGAGGGCTT CTCTGAAGGA 
CGGCTGCTGT CCCCAGTGGC TGCTGCCTCA GCAGCCCGGC AAGACCCTGT AGAGCTGTTG 
CTGCTGTCCA CCCAGGAACG ACTGGCCGCG GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACTATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TTGACTCACG CCGTTCCTCA 
GGCTTCCGCA GCATCAGAGG CTCACCCAGC CTCCATGCAG TACTGGAGCG TGATGAGGGC 
CACCTGTTTT ACATCGACCC AGCCATTCCA GAGGAGAACC CATCCCTCAG CTCCCGCACA 
GAGCTGCTGG CAGCTGACAG TCTGTCCAAG CACTCGCAGG ACACCCAACC CCTGGAGGCA 
GCACTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAGCCAA TGACGAGGAG 
GAGGCAGCCG GTGGCAGTGA GGGTGGTGGG GTGGCCCCAC GAGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGATTC GCCCAGCCCT CAGGTGCTAG AATTCGCTGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTGTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACAGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAATCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCCGAAC CCTCCTCTGG CATCTCACTC TCACCCTCCT TCCCACTCAG CTCCTCAGGG 
GAGCTCATGG ACCTGACACC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCATGGG GCCAGCCCTA CCAAGCAGGA TGACCTGGTC 
ATCTCAGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Uncoupling of the endocannabinoid signalling complex in a mouse model of fragile X syndrome.
Nature communications31080(2012)
Jung KM,Sepers M,Henstridge CM,Lassalle O,Neuhofer D,Martin H,Ginger M,Frick A,DiPatrizio NV,Mackie K,Katona I,Piomelli D,Manzoni OJ

Distribution of diacylglycerol lipase alpha, an endocannabinoid synthesizing enzyme, in the rat forebrain.
Neuroscience192112-31(2011 Sep)
Su?rez J,Ort?z O,Puente N,Berm?dez-Silva FJ,Blanco E,Fern?ndez-Llebrez P,Grandes P,de Fonseca FR,Moratalla R

Endocannabinoids generated by Ca2+ or by metabotropic glutamate receptors appear to arise from different pools of diacylglycerol lipase.
PloS one6(1)e16305(2011 Jan)
Zhang L,Wang M,Bisogno T,Di Marzo V,Alger BE

Differential subcellular recruitment of monoacylglycerol lipase generates spatial specificity of 2-arachidonoyl glycerol signaling during axonal pathfinding.
The Journal of neuroscience : the official journal of the Society for Neuroscience30(42)13992-4007(2010 Oct)
Keimpema E,Barabas K,Morozov YM,Tortoriello G,Torii M,Cameron G,Yanagawa Y,Watanabe M,Mackie K,Harkany T

Early maternal deprivation induces changes on the expression of 2-AG biosynthesis and degradation enzymes in neonatal rat hippocampus.
Brain research1349162-73(2010 Aug)
Su?rez J,Rivera P,Llorente R,Romero-Zerbo SY,Berm?dez-Silva FJ,de Fonseca FR,Viveros MP