Egln1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Egln1
Entrez Gene ID	308913
Full Name	egl-9 family hypoxia-inducible factor 1
Synonyms	HIF-PH2,HPH-2,PHD-2,PHD2
General protein information	Preferred Names egl-9 family hypoxia-inducible factor 1 Names egl nine homolog 1 LOW QUALITY PROTEIN: egl nine homolog 1 HIF-prolyl hydroxylase 2 hypoxia-inducible factor prolyl hydroxylase 2 prolyl hydroxylase domain-containing protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_002725436.3	XP_002725482.2	egl nine homolog 1 isoform X2
XM_008772679.1	XP_008770901.1	LOW QUALITY PROTEIN: egl nine homolog 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EGLN1	NP_071334.1
Pan troglodytes (chimpanzee)	EGLN1	XP_525092.2
Canis lupus familiaris (dog)	EGLN1	XP_546089.3
Rattus norvegicus (Norway rat)	Egln1	XP_002728657.3
Danio rerio (zebrafish)	si:ch1073-423n4.1	XP_002664281.2
Mus musculus (house mouse)	Egln1	NP_444437.2
	EGLN1	XP_001104870.1
Danio rerio (zebrafish)	egln1	NP_001002595.2
Gallus gallus (chicken)	LOC768374	XP_001231254.3
Xenopus tropicalis (tropical clawed frog)	egln1	NP_001015960.1

Gene Ontology

The following Egln1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Egln1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa46672	XM_008772679.1 Latest version!	Rattus norvegicus egl-9 family hypoxia-inducible factor 1 (Egln1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Egln1 ( XM_008772679.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770901.1 Rattus norvegicus egl-9 family hypoxia-inducible factor 1 (Egln1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa46672
Clone ID Related Accession (Same CDS sequence)	XM_008772679.1
Accession Version	XM_008772679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: egl nine homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 33% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGAGGGCTA ACGCTAATCA CCTTACCATT TTGGCAGAAG AGGTAGGAGA GTCTATGTCC 
GAGGACTACC GGGCTGCGTC TCAAGACAAC AAAGAAAGCC CCAGGCCCGG AGCGCGCGAC 
CGGCCGCCCG GGAGCTCGGA GCGGCCCAGA GGCCGCCTGA GCTTCCGTTT CCTCCTCCGC 
GACGACCCGA GGTGGCCGAG GCACCCGGAG CGCGGCCCGT GGGCCCCGAC AGGCAGGTGG 
AGCGCGCGCC GGCGCGCGCG CGCGCCCAGG CCGGCGACGC GCGCCCCGGC GGAGCCGCGA 
GGCGGAGGGA GGCGGAGAAG CGGCGCGCCC GCCGCTAGGC CCGGCCCCGT GCCGCGTGCG 
CGCGGCCGCC CGCCTTCCGC CCGCGCCGGT CGCCGGGGCC GTGTGTACGT GCACCGCGGA 
GCGAGTGGCA GCCCGCAGCC CGCGCCCTGC CCGCGCCGCC CCGACCAGCC GGCTGGGCCG 
CCCGCCAGGG CCGTGCGCCG CGGCAGGCCA AGCGCTCTCA TCCGGGCGCG ATGCGGCGCT 
AGGCGGCCCG GGGCGAAGGC AGGCGAGGCA GGGCGCGCGC GGCCTCGCGC AGCGGGCGGC 
CCCCGGCGGC GCCCCGACGG CCGACCGCCG CCCCGCTCCG GCCGGCGGCC CGCCCTGCCG 
CGGCCATGGC CCAGTGACAG CGGCGGGCCC GCGGTGCTGA GCGCCAGCGA GCGCGAGCGG 
CAGTACTGCG AGCTGTGCGT GAAGATGCGA AACCTGTTGC GCCTGCGGCC GCGCCGCAGC 
TCCCAAAATA TTAAACTCTG CTGCAGGAGC ACCAGCGACC AGGACGGGAA GAAGCACAAG 
GCTGATGGTG CCCAGGGCGG CGAGGCCCCC CGCGCGCAGC CCGCGCCAGC GCAGCCCCGC 
GTCGCGCCCC CGCCCGGTGG GGCCCCCGGA GCCGCGCGCG CCGGCGGGGC GGCCCGGCGA 
GGGGACAGCA GCACGGCGGC CTCGCGCGTA CCGGGCCCGG AGGACGCGAC GCAGGCCGGG 
AGCGGCCCCG GCCCGGCAGA GCCCAGCTCC GAGGATCCTC CGCCTAGCAG GTCTCCGGGC 
CCCGAGCGCG CTAGCTTGTG CCCAGCGGGC GGCGGCCCCG GGGAGGCGCT GAGTCCCAGC 
GGAGGGCTGC GGCCCAACGG GCAGACCAAG CCGCTGCCCG CGCTGAAGCT GGCGCTGGAG 
TACATCGTGC CGTGCATGAA CAAGCACGGC ATCTGCGTGG TGGACGACTT CCTGGGCAGG 
GAGACCGGGC AGCAGATCGG CGACGAGGTG CGCGCCCTGC ACGACACCGG CAAGTTCACG 
GACGGGCAAC TGGTCAGCCA GAAGAGTGAC TCTTCCAAGG ACATCCGGGG GGACAAGATC 
ACCTGGATCG AGGGCAAAGA GCCCGGCTGC GAGACCATCG GCCTGCTCAT GAGCAGCATG 
GACGACCTGA TCCGCCACTG CAGCGGGAAG CTGGGCAACT ACAGGATAAA CGGCCGAACG 
AAAGCCATGG TCGCCTGTTA CCCAGGCAAC GGGACAGGCT ACGTCCGTCA CGTCGATAAC 
CCAAATGGAG ACGGAAGATG TGTGACATGT ATATATTATC TAAATAAAGA CTGGGACGCC 
AAGGTGAGCG GAGGTATTCT TCGAATTTTC CCAGAAGGCA AAGCCCAGTT TGCTGACATT 
GAACCCAAGT TTGATAGATT GCTGTTTTTC TGGTCTGACC GGCGTAACCC TCATGAAGTA 
CAGCCTGCAT ACGCCACAAG GTACGCAATA ACCGTTTGGT ATTTCGATGC AGACGAGCGA 
GCAAGAGCTA AAGTAAAATA TCTCACAGGT GAGAAAGGTG TGAGGGTTGA ACTCAAGCCC 
AATTCAGTCA GCAAAGATGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770901.1
CDS	1..1884
Translation	MRANANHLTILAEEVGESMSEDYRAASQDNKESPRPGARDRPPGSSERPRGRLSFRFLLRDDPRWPRHPERGPWAPTGRWSARRRARAPRPATRAPAEPRGGGRRRSGAPAARPGPVPRARGRPPSARAGRRGRVYVHRGASGSPQPAPCPRRPDQPAGPPARAVRRGRPSALIRARCGARRPGAKAGEAGRARPRAAGGPRRRPDGRPPPRSGRRPALPRPWPSDSGGPAVLSASERERQYCELCVKMRNLLRLRPRRSSQNIKLCCRSTSDQDGKKHKADGAQGGEAPRAQPAPAQPRVAPPPGGAPGAARAGGAARRGDSSTAASRVPGPEDATQAGSGPGPAEPSSEDPPPSRSPGPERASLCPAGGGPGEALSPSGGLRPNGQTKPLPALKLALEYIVPCMNKHGICVVDDFLGRETGQQIGDEVRALHDTGKFTDGQLVSQKSDSSKDIRGDKITWIEGKEPGCETIGLLMSSMDDLIRHCSGKLGNYRINGRTKAMVACYPGNGTGYVRHVDNPNGDGRCVTCIYYLNKDWDAKVSGGILRIFPEGKAQFADIEPKFDRLLFFWSDRRNPHEVQPAYATRYAITVWYFDADERARAKVKYLTGEKGVRVELKPNSVSKDV

Target ORF information:

RefSeq Version	XM_008772679.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus egl-9 family hypoxia-inducible factor 1 (Egln1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772679.1

ORF Insert Sequence:

ATGAGGGCTA ACGCTAATCA CCTTACCATT TTGGCAGAAG AGGTAGGAGA GTCTATGTCC 
GAGGACTACC GGGCTGCGTC TCAAGACAAC AAAGAAAGCC CCAGGCCCGG AGCGCGCGAC 
CGGCCGCCCG GGAGCTCGGA GCGGCCCAGA GGCCGCCTGA GCTTCCGTTT CCTCCTCCGC 
GACGACCCGA GGTGGCCGAG GCACCCGGAG CGCGGCCCGT GGGCCCCGAC AGGCAGGTGG 
AGCGCGCGCC GGCGCGCGCG CGCGCCCAGG CCGGCGACGC GCGCCCCGGC GGAGCCGCGA 
GGCGGAGGGA GGCGGAGAAG CGGCGCGCCC GCCGCTAGGC CCGGCCCCGT GCCGCGTGCG 
CGCGGCCGCC CGCCTTCCGC CCGCGCCGGT CGCCGGGGCC GTGTGTACGT GCACCGCGGA 
GCGAGTGGCA GCCCGCAGCC CGCGCCCTGC CCGCGCCGCC CCGACCAGCC GGCTGGGCCG 
CCCGCCAGGG CCGTGCGCCG CGGCAGGCCA AGCGCTCTCA TCCGGGCGCG ATGCGGCGCT 
AGGCGGCCCG GGGCGAAGGC AGGCGAGGCA GGGCGCGCGC GGCCTCGCGC AGCGGGCGGC 
CCCCGGCGGC GCCCCGACGG CCGACCGCCG CCCCGCTCCG GCCGGCGGCC CGCCCTGCCG 
CGGCCATGGC CCAGTGACAG CGGCGGGCCC GCGGTGCTGA GCGCCAGCGA GCGCGAGCGG 
CAGTACTGCG AGCTGTGCGT GAAGATGCGA AACCTGTTGC GCCTGCGGCC GCGCCGCAGC 
TCCCAAAATA TTAAACTCTG CTGCAGGAGC ACCAGCGACC AGGACGGGAA GAAGCACAAG 
GCTGATGGTG CCCAGGGCGG CGAGGCCCCC CGCGCGCAGC CCGCGCCAGC GCAGCCCCGC 
GTCGCGCCCC CGCCCGGTGG GGCCCCCGGA GCCGCGCGCG CCGGCGGGGC GGCCCGGCGA 
GGGGACAGCA GCACGGCGGC CTCGCGCGTA CCGGGCCCGG AGGACGCGAC GCAGGCCGGG 
AGCGGCCCCG GCCCGGCAGA GCCCAGCTCC GAGGATCCTC CGCCTAGCAG GTCTCCGGGC 
CCCGAGCGCG CTAGCTTGTG CCCAGCGGGC GGCGGCCCCG GGGAGGCGCT GAGTCCCAGC 
GGAGGGCTGC GGCCCAACGG GCAGACCAAG CCGCTGCCCG CGCTGAAGCT GGCGCTGGAG 
TACATCGTGC CGTGCATGAA CAAGCACGGC ATCTGCGTGG TGGACGACTT CCTGGGCAGG 
GAGACCGGGC AGCAGATCGG CGACGAGGTG CGCGCCCTGC ACGACACCGG CAAGTTCACG 
GACGGGCAAC TGGTCAGCCA GAAGAGTGAC TCTTCCAAGG ACATCCGGGG GGACAAGATC 
ACCTGGATCG AGGGCAAAGA GCCCGGCTGC GAGACCATCG GCCTGCTCAT GAGCAGCATG 
GACGACCTGA TCCGCCACTG CAGCGGGAAG CTGGGCAACT ACAGGATAAA CGGCCGAACG 
AAAGCCATGG TCGCCTGTTA CCCAGGCAAC GGGACAGGCT ACGTCCGTCA CGTCGATAAC 
CCAAATGGAG ACGGAAGATG TGTGACATGT ATATATTATC TAAATAAAGA CTGGGACGCC 
AAGGTGAGCG GAGGTATTCT TCGAATTTTC CCAGAAGGCA AAGCCCAGTT TGCTGACATT 
GAACCCAAGT TTGATAGATT GCTGTTTTTC TGGTCTGACC GGCGTAACCC TCATGAAGTA 
CAGCCTGCAT ACGCCACAAG GTACGCAATA ACCGTTTGGT ATTTCGATGC AGACGAGCGA 
GCAAGAGCTA AAGTAAAATA TCTCACAGGT GAGAAAGGTG TGAGGGTTGA ACTCAAGCCC 
AATTCAGTCA GCAAAGATGT CTAG