Nipa1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nipa1
Entrez Gene ID	308668
Full Name	non imprinted in Prader-Willi/Angelman syndrome 1
Synonyms	SLC56A1,Spg6
General protein information	Preferred Names non imprinted in Prader-Willi/Angelman syndrome 1 Names magnesium transporter NIPA1 non imprinted in Prader-Willi/Angelman syndrome 1 homolog solute carrier family 56 member 1 spastic paraplegia 6 (autosomal dominant)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107519.1	NP_001100989.1	magnesium transporter NIPA1
XM_017589155.1	XP_017444644.1	magnesium transporter NIPA1 isoform X1

Pathways from BioSystems

Homologies

	LOC710236	XP_001106326.1
Danio rerio (zebrafish)	nipa1	NP_001006062.1
Homo sapiens (human)	NIPA1	NP_653200.2
Mus musculus (house mouse)	Nipa1	NP_705806.1
Rattus norvegicus (Norway rat)	Nipa1	NP_001100989.1
Gallus gallus (chicken)	NIPA1	XP_003640574.2
Xenopus tropicalis (tropical clawed frog)	nipa1	XP_002937031.1
Canis lupus familiaris (dog)	NIPA1	XP_545798.2
Bos taurus (cattle)	NIPA1	XP_002685238.3

Gene Ontology
Bibliography

Related articles in PubMed

The effect of HSP-causing mutations in SPG3A and NIPA1 on the assembly, trafficking, and interaction between atlastin-1 and NIPA1.
Botzolakis EJ, Zhao J, Gurba KN, Macdonald RL, Hedera P
Molecular and cellular neurosciences46(1)122-35(2011 Jan)

NIPA1(SPG6), the basis for autosomal dominant form of hereditary spastic paraplegia, encodes a functional Mg2+ transporter.
Goytain A, Hines RM, El-Husseini A, Quamme GA
The Journal of biological chemistry282(11)8060-8(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that NIPA1 and atlastin-1 are members of a common biochemical pathway that supports axonal maintenance, which may explain in part the characteristic degeneration of long spinal pathways observed in patients with HSP.
Title: The effect of HSP-causing mutations in SPG3A and NIPA1 on the assembly, trafficking, and interaction between atlastin-1 and NIPA1.

The following Nipa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nipa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa56585	XM_017589155.1 Latest version!	Rattus norvegicus non imprinted in Prader-Willi/Angelman syndrome 1 (Nipa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa03807	NM_001107519.1 Latest version!	Rattus norvegicus NIPA magnesium transporter 1 (Nipa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nipa1 ( XM_017589155.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444644.1 Rattus norvegicus non imprinted in Prader-Willi/Angelman syndrome 1 (Nipa1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56585
Clone ID Related Accession (Same CDS sequence)	XM_017589155.1
Accession Version	XM_017589155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	magnesium transporter NIPA1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTG GCCAGATTGG AAACTTCCTA GCTTACACTG CGGTCCCTAC GGTCCTGGTG 
ACTCCTTTAG GTGCCCTTGG AGTGCCATTT GGGTCCATTT TAGCTTCTTA CCTTCTGAAG 
GAGAAGCTCA ACATCTTGGG CAAGTTGGGG TGTCTGCTAA GCTGCGCAGG GTCTGTCGTG 
CTGATCATCC ATTCCCCCAA GTCCGAGAGC GTGACCAGTC AGGCTGAGCT GGAGGAGAAG 
CTGACCAATC CAGTGTTTGT GGGCTACCTG TGCATTGTGC TGCTCATGCT GCTGCTGTTG 
ATCTTCTGGA TTGCACCAGC CCACGGGCCC ACCAACATCA TGGTCTACAT CAGCATCTGC 
TCCTTGCTGG GCAGTTTCAC TGTGCCTTCC ACCAAGGGCA TTGGGTTGGC AGCCCAAGAC 
ATCTTGCACA ACAATCCGTC CAGTCAGAGA GCCCTCTGCC TGTGCCTGGT GCTCCTGGCT 
GTCCTGGGCT GCAGCATCAT TGTCCAATTC AGGTACATCA ACAAGGCCCT GGAGTGCTTC 
GACTCCTCTG TGTTTGGGGC CATCTACTAC GTTGTGTTTA CCACGCTGGT CCTGCTGGCT 
TCAGCCATCC TCTTCCGGGA GTGGAGCAAT GTGGGCCTGG TGGACTTCTT GGGCATGGCC 
TGTGGATTTA CAACTGTCTC CGTGGGAATT GTCCTTATAC AAGTGTTCAA AGAATTCAAC 
TTCAACCTTG GGGAGATGAA CAAATCCAAT ATGAAAACAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444644.1
CDS	84..848
Translation	MAVGQIGNFLAYTAVPTVLVTPLGALGVPFGSILASYLLKEKLNILGKLGCLLSCAGSVVLIIHSPKSESVTSQAELEEKLTNPVFVGYLCIVLLMLLLLIFWIAPAHGPTNIMVYISICSLLGSFTVPSTKGIGLAAQDILHNNPSSQRALCLCLVLLAVLGCSIIVQFRYINKALECFDSSVFGAIYYVVFTTLVLLASAILFREWSNVGLVDFLGMACGFTTVSVGIVLIQVFKEFNFNLGEMNKSNMKTD

Target ORF information:

RefSeq Version	XM_017589155.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus non imprinted in Prader-Willi/Angelman syndrome 1 (Nipa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589155.1

ORF Insert Sequence:

ATGGCTGTTG GCCAGATTGG AAACTTCCTA GCTTACACTG CGGTCCCTAC GGTCCTGGTG 
ACTCCTTTAG GTGCCCTTGG AGTGCCATTT GGGTCCATTT TAGCTTCTTA CCTTCTGAAG 
GAGAAGCTCA ACATCTTGGG CAAGTTGGGG TGTCTGCTAA GCTGCGCAGG GTCTGTCGTG 
CTGATCATCC ATTCCCCCAA GTCCGAGAGC GTGACCAGTC AGGCTGAGCT GGAGGAGAAG 
CTGACCAATC CAGTGTTTGT GGGCTACCTG TGCATTGTGC TGCTCATGCT GCTGCTGTTG 
ATCTTCTGGA TTGCACCAGC CCACGGGCCC ACCAACATCA TGGTCTACAT CAGCATCTGC 
TCCTTGCTGG GCAGTTTCAC TGTGCCTTCC ACCAAGGGCA TTGGGTTGGC AGCCCAAGAC 
ATCTTGCACA ACAATCCGTC CAGTCAGAGA GCCCTCTGCC TGTGCCTGGT GCTCCTGGCT 
GTCCTGGGCT GCAGCATCAT TGTCCAATTC AGGTACATCA ACAAGGCCCT GGAGTGCTTC 
GACTCCTCTG TGTTTGGGGC CATCTACTAC GTTGTGTTTA CCACGCTGGT CCTGCTGGCT 
TCAGCCATCC TCTTCCGGGA GTGGAGCAAT GTGGGCCTGG TGGACTTCTT GGGCATGGCC 
TGTGGATTTA CAACTGTCTC CGTGGGAATT GTCCTTATAC AAGTGTTCAA AGAATTCAAC 
TTCAACCTTG GGGAGATGAA CAAATCCAAT ATGAAAACAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa1 ( NM_001107519.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100989.1 Rattus norvegicus NIPA magnesium transporter 1 (Nipa1), mRNA.

Price & Availability↑

CloneID	ORa03807
Clone ID Related Accession (Same CDS sequence)	NM_001107519.1
Accession Version	NM_001107519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	magnesium transporter NIPA1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474036.2. On or before Oct 4, 2007 this sequence version replaced XM_218719.4, XM_001054918.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGGGACTG CGGCGGCGGC TGCGGCGGCC GGGGAGGGGG CGCGCGGCCC GAGCCCCGCC 
GCCGTGTCGC TCGGCCTGGG CGTGGCTGTG GTGTCGAGCC TAGTGAACGG GTCCACGTTC 
GTGCTGCAGA AGAAGGGCAT TGTGCGCGCT AAAAGGCGAG GTACTTCCTA CTTAACAGAT 
ATCGTGTGGT GGGCTGGCAC CATTGCAATG GCTGTTGGCC AGATTGGAAA CTTCCTAGCT 
TACACTGCGG TCCCTACGGT CCTGGTGACT CCTTTAGGTG CCCTTGGAGT GCCATTTGGG 
TCCATTTTAG CTTCTTACCT TCTGAAGGAG AAGCTCAACA TCTTGGGCAA GTTGGGGTGT 
CTGCTAAGCT GCGCAGGGTC TGTCGTGCTG ATCATCCATT CCCCCAAGTC CGAGAGCGTG 
ACCAGTCAGG CTGAGCTGGA GGAGAAGCTG ACCAATCCAG TGTTTGTGGG CTACCTGTGC 
ATTGTGCTGC TCATGCTGCT GCTGTTGATC TTCTGGATTG CACCAGCCCA CGGGCCCACC 
AACATCATGG TCTACATCAG CATCTGCTCC TTGCTGGGCA GTTTCACTGT GCCTTCCACC 
AAGGGCATTG GGTTGGCAGC CCAAGACATC TTGCACAACA ATCCGTCCAG TCAGAGAGCC 
CTCTGCCTGT GCCTGGTGCT CCTGGCTGTC CTGGGCTGCA GCATCATTGT CCAATTCAGG 
TACATCAACA AGGCCCTGGA GTGCTTCGAC TCCTCTGTGT TTGGGGCCAT CTACTACGTT 
GTGTTTACCA CGCTGGTCCT GCTGGCTTCA GCCATCCTCT TCCGGGAGTG GAGCAATGTG 
GGCCTGGTGG ACTTCTTGGG CATGGCCTGT GGATTTACAA CTGTCTCCGT GGGAATTGTC 
CTTATACAAG TGTTCAAAGA ATTCAACTTC AACCTTGGGG AGATGAACAA ATCCAATATG 
AAAACAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100989.1
CDS	7..978
Translation	MGTAAAAAAAGEGARGPSPAAVSLGLGVAVVSSLVNGSTFVLQKKGIVRAKRRGTSYLTDIVWWAGTIAMAVGQIGNFLAYTAVPTVLVTPLGALGVPFGSILASYLLKEKLNILGKLGCLLSCAGSVVLIIHSPKSESVTSQAELEEKLTNPVFVGYLCIVLLMLLLLIFWIAPAHGPTNIMVYISICSLLGSFTVPSTKGIGLAAQDILHNNPSSQRALCLCLVLLAVLGCSIIVQFRYINKALECFDSSVFGAIYYVVFTTLVLLASAILFREWSNVGLVDFLGMACGFTTVSVGIVLIQVFKEFNFNLGEMNKSNMKTD

Target ORF information:

RefSeq Version	NM_001107519.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NIPA magnesium transporter 1 (Nipa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107519.1

ORF Insert Sequence:

ATGGGGACTG CGGCGGCGGC TGCGGCGGCC GGGGAGGGGG CGCGCGGCCC GAGCCCCGCC 
GCCGTGTCGC TCGGCCTGGG CGTGGCTGTG GTGTCGAGCC TAGTGAACGG GTCCACGTTC 
GTGCTGCAGA AGAAGGGCAT TGTGCGCGCT AAAAGGCGAG GTACTTCCTA CTTAACAGAT 
ATCGTGTGGT GGGCTGGCAC CATTGCAATG GCTGTTGGCC AGATTGGAAA CTTCCTAGCT 
TACACTGCGG TCCCTACGGT CCTGGTGACT CCTTTAGGTG CCCTTGGAGT GCCATTTGGG 
TCCATTTTAG CTTCTTACCT TCTGAAGGAG AAGCTCAACA TCTTGGGCAA GTTGGGGTGT 
CTGCTAAGCT GCGCAGGGTC TGTCGTGCTG ATCATCCATT CCCCCAAGTC CGAGAGCGTG 
ACCAGTCAGG CTGAGCTGGA GGAGAAGCTG ACCAATCCAG TGTTTGTGGG CTACCTGTGC 
ATTGTGCTGC TCATGCTGCT GCTGTTGATC TTCTGGATTG CACCAGCCCA CGGGCCCACC 
AACATCATGG TCTACATCAG CATCTGCTCC TTGCTGGGCA GTTTCACTGT GCCTTCCACC 
AAGGGCATTG GGTTGGCAGC CCAAGACATC TTGCACAACA ATCCGTCCAG TCAGAGAGCC 
CTCTGCCTGT GCCTGGTGCT CCTGGCTGTC CTGGGCTGCA GCATCATTGT CCAATTCAGG 
TACATCAACA AGGCCCTGGA GTGCTTCGAC TCCTCTGTGT TTGGGGCCAT CTACTACGTT 
GTGTTTACCA CGCTGGTCCT GCTGGCTTCA GCCATCCTCT TCCGGGAGTG GAGCAATGTG 
GGCCTGGTGG ACTTCTTGGG CATGGCCTGT GGATTTACAA CTGTCTCCGT GGGAATTGTC 
CTTATACAAG TGTTCAAAGA ATTCAACTTC AACCTTGGGG AGATGAACAA ATCCAATATG 
AAAACAGACT AA