Cyb5r1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyb5r1
Entrez Gene ID	304805
Full Name	cytochrome b5 reductase 1
Synonyms	Nqo3a2
General protein information	Preferred Names cytochrome b5 reductase 1 Names NADH-cytochrome b5 reductase 1 NAD(P)H:quinone oxidoreductase type 3, polypeptide A2 b5R.1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013126.1	NP_001013144.1	NADH-cytochrome b5 reductase 1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100488016	XP_002939683.2
Homo sapiens (human)	CYB5R1	NP_057327.2
Bos taurus (cattle)	CYB5R1	NP_001029690.1
Mus musculus (house mouse)	Cyb5r1	NP_082333.1
	CYB5R1	XP_001105252.1
Rattus norvegicus (Norway rat)	Cyb5r1	NP_001013144.1
Magnaporthe oryzae (rice blast fungus)	MGG_14956	XP_003720314.1
Pan troglodytes (chimpanzee)	LOC740001	XP_001141761.2
Danio rerio (zebrafish)	cyb5r1	NP_956483.1
Neurospora crassa	NCU08060	XP_965191.1

Gene Ontology
Bibliography

Related articles in PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.
Samhan-Arias AK, Marques-da-Silva D, Yanamala N, Gutierrez-Merino C
Journal of proteomics75(10)2934-49(2012 Jun)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis.
Title: Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.

The following Cyb5r1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyb5r1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03466	NM_001013126.1 Latest version!	Rattus norvegicus cytochrome b5 reductase 1 (Cyb5r1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyb5r1 ( NM_001013126.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013144.1 Rattus norvegicus cytochrome b5 reductase 1 (Cyb5r1), mRNA.

Price & Availability↑

CloneID	ORa03466
Clone ID Related Accession (Same CDS sequence)	NM_001013126.1
Accession Version	NM_001013126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	NADH-cytochrome b5 reductase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089945.1. On Apr 20, 2005 this sequence version replaced XM_222644.3. ##Evidence-Data-START## Transcript exon combination :: BC089945.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGATCC AGCCGAGCCC GGTCTTGCTA GCCTCTCTGG GGGTGGGGCT GTTTACCCTG 
TTCGGCCTGG CTCTGGGTAC CTACTTGGTT CGAAGATCCC GCCGGCCACA GGTCACTCTC 
CAGGACCCGG ATGAGAAGTA CCTGCTGCGA TTGCTGGACA AGACGACAGT GAGCCACAAC 
ACCAGGAGGT TCCGCTTTGC CCTGCCCACC GCCCACCACA TTCTGGGGCT GCCTGTGGGT 
AAGCACGTCT ACCTCTCTGC CCGAATTGAT GGCAGTCAGG TCATCAGGCC TTACACTCCT 
GTCACCAGCG ATGAAGACCA GGGCTATGTA GATCTTGTCA TCAAGGTTTA TCTGAAAGGT 
GTGCACCCCA AATTTTCTGA AGGAGGGAAG ATGTCTCAGT ACCTGGATAG CCTGAAGATT 
GGGGATGTGG TGGAGTTCCG GGGGCCGAGT GGGTTGCTCA GTTATGCTGG GAAAGGGAAT 
TTTAACATTC AGCCCAACAA AAAATCCCCA CCTGAACTGC GAGTGGCTAA GAAATTGGGA 
ATGATTGCTG GCGGGACAGG AATCACCCCA ATGCTGCAGC TGATCCGGGC CATCTTGAAA 
GTCCCTGAAG ATCCAACCCA GTGCTTTCTG CTTTTTGCCA ACCAGACTGA AAAGGACATA 
ATCCTGCGGG AGGACCTAGA GGAGCTGCAG GCCCAATATC CTATCCGCTT TAAGCTCTGG 
TTCACCCTGG ACTACCCTCC AGAAGACTGG ACCTACAGCA AGGGCTTTGT TACCGCCGAC 
ATGATCCAGG AGCACCTGCC TGCCCCAGCA GAGGATGTGC TGCTGCTGCT TTGTGGGCCA 
CCACCTATGG TACAGCTGGC CTGCCACCCG AACCTGGATA AGCTGGGCTA TTCACAGAAG 
ATGCGATTCA CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013144.1
CDS	45..962
Translation	MGIQPSPVLLASLGVGLFTLFGLALGTYLVRRSRRPQVTLQDPDEKYLLRLLDKTTVSHNTRRFRFALPTAHHILGLPVGKHVYLSARIDGSQVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFSEGGKMSQYLDSLKIGDVVEFRGPSGLLSYAGKGNFNIQPNKKSPPELRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFLLFANQTEKDIILREDLEELQAQYPIRFKLWFTLDYPPEDWTYSKGFVTADMIQEHLPAPAEDVLLLLCGPPPMVQLACHPNLDKLGYSQKMRFTY

Target ORF information:

RefSeq Version	NM_001013126.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome b5 reductase 1 (Cyb5r1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013126.1

ORF Insert Sequence:

ATGGGGATCC AGCCGAGCCC GGTCTTGCTA GCCTCTCTGG GGGTGGGGCT GTTTACCCTG 
TTCGGCCTGG CTCTGGGTAC CTACTTGGTT CGAAGATCCC GCCGGCCACA GGTCACTCTC 
CAGGACCCGG ATGAGAAGTA CCTGCTGCGA TTGCTGGACA AGACGACAGT GAGCCACAAC 
ACCAGGAGGT TCCGCTTTGC CCTGCCCACC GCCCACCACA TTCTGGGGCT GCCTGTGGGT 
AAGCACGTCT ACCTCTCTGC CCGAATTGAT GGCAGTCAGG TCATCAGGCC TTACACTCCT 
GTCACCAGCG ATGAAGACCA GGGCTATGTA GATCTTGTCA TCAAGGTTTA TCTGAAAGGT 
GTGCACCCCA AATTTTCTGA AGGAGGGAAG ATGTCTCAGT ACCTGGATAG CCTGAAGATT 
GGGGATGTGG TGGAGTTCCG GGGGCCGAGT GGGTTGCTCA GTTATGCTGG GAAAGGGAAT 
TTTAACATTC AGCCCAACAA AAAATCCCCA CCTGAACTGC GAGTGGCTAA GAAATTGGGA 
ATGATTGCTG GCGGGACAGG AATCACCCCA ATGCTGCAGC TGATCCGGGC CATCTTGAAA 
GTCCCTGAAG ATCCAACCCA GTGCTTTCTG CTTTTTGCCA ACCAGACTGA AAAGGACATA 
ATCCTGCGGG AGGACCTAGA GGAGCTGCAG GCCCAATATC CTATCCGCTT TAAGCTCTGG 
TTCACCCTGG ACTACCCTCC AGAAGACTGG ACCTACAGCA AGGGCTTTGT TACCGCCGAC 
ATGATCCAGG AGCACCTGCC TGCCCCAGCA GAGGATGTGC TGCTGCTGCT TTGTGGGCCA 
CCACCTATGG TACAGCTGGC CTGCCACCCG AACCTGGATA AGCTGGGCTA TTCACAGAAG 
ATGCGATTCA CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.
Journal of proteomics75(10)2934-49(2012 Jun)
Samhan-Arias AK,Marques-da-Silva D,Yanamala N,Gutierrez-Merino C

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR