Vpreb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vpreb1
Entrez Gene ID	303782
Full Name	V-set pre-B cell surrogate light chain 1
General protein information	Preferred Names V-set pre-B cell surrogate light chain 1 Names immunoglobulin iota chain Immunoglobulin lambda Vpreb1 chain immunoglobulin omega chain-like pre-B lymphocyte 1 pre-B lymphocyte gene 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	mouse homolog forms a surrogate light chain in the pre-B cell receptor and plays a role in B-cell proliferation and expansion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108845.1	NP_001102315.1	immunoglobulin iota chain precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vpreb2	NP_001128260.1
Mus musculus (house mouse)	Vpreb2	NP_058679.1
Canis lupus familiaris (dog)	VPREB1	XP_543537.2
Mus musculus (house mouse)	Vpreb1	NP_058678.1
Rattus norvegicus (Norway rat)	Vpreb1	NP_001102315.1
Homo sapiens (human)	VPREB1	NP_009059.1
Pan troglodytes (chimpanzee)	VPREB1	XP_515010.2
	VPREB1	XP_001089831.1

Gene Ontology
Bibliography

Related articles in PubMed

Both the pre-BCR and the IL-7Ralpha are essential for expansion at the pre-BII cell stage in vivo.
Erlandsson L, Licence S, Gaspal F, Lane P, Corcoran AE, M?rtensson IL
European journal of immunology35(6)1969-76(2005 Jun)

Gene and alternative splicing annotation with AIR.
Florea L, Di Francesco V, Miller J, Turner R, Yao A, Harris M, Walenz B, Mobarry C, Merkulov GV, Charlab R, Dew I, Deng Z, Istrail S, Li P, Sutton G
Genome research15(1)54-66(2005 Jan)

VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus.
Shimizu T, Mundt C, Licence S, Melchers F, M?rtensson IL
Journal of immunology (Baltimore, Md. : 1950)168(12)6286-93(2002 Jun)

Partial block in B lymphocyte development at the transition into the pre-B cell receptor stage in Vpre-B1-deficient mice.
M?rtensson A, Argon Y, Melchers F, Dul JL, M?rtensson IL
International immunology11(3)453-60(1999 Mar)

The murine VpreB1 and VpreB2 genes both encode a protein of the surrogate light chain and are co-expressed during B cell development.
Dul JL, Argon Y, Winkler T, ten Boekel E, Melchers F, M?rtensson IL
European journal of immunology26(4)906-13(1996 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vpreb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vpreb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08486	NM_001108845.1 Latest version!	Rattus norvegicus V-set pre-B cell surrogate light chain 1 (Vpreb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vpreb1 ( NM_001108845.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102315.1 Rattus norvegicus V-set pre-B cell surrogate light chain 1 (Vpreb1), mRNA.

Price & Availability↑

CloneID	ORa08486
Clone ID Related Accession (Same CDS sequence)	NM_001108845.1
Accession Version	NM_001108845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin iota chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473999.2. On or before Oct 4, 2007 this sequence version replaced XM_577195.2, XM_001062818.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689605, SAMN00632259 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTGGA CGTCTGCCCT GCTCATACTG CTGGCCCATC TCACAGGTTG TGGCCCTCAG 
CCCGTGCTGC ATCAGCCACC ATCGGCCTCT TCCTTCCTTG GAACCTCCAT CCGCCTCACC 
TGTGCCCTGA GCAGCAACCA TAACATTGGC ATTTACAGCA TTTACTGGTA CCAGCAGAGG 
CCGGGCCACC CTCCCACGTT CCTGCTGAGA TTCTTCTCAC ACTCAGACAA GCTCCAGGGT 
CCCAAGATCC CCCCTCGCTT CTCCGGATCC AAAGATACAG CCAGGAACCT GGGGTACCTG 
AGCATCTCTG ACCTGCAGCC AGAGGACGAG GCTGTGTATT ACTGCGCCGT GGGGCTTCGG 
AGCTGGGAAA AGGAGAAGAG GATGGAGAGG GAGTGGGAAG AAGAAAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102315.1
CDS	125..535
Translation	MAWTSALLILLAHLTGCGPQPVLHQPPSASSFLGTSIRLTCALSSNHNIGIYSIYWYQQRPGHPPTFLLRFFSHSDKLQGPKIPPRFSGSKDTARNLGYLSISDLQPEDEAVYYCAVGLRSWEKEKRMEREWEEEK

Target ORF information:

RefSeq Version	NM_001108845.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus V-set pre-B cell surrogate light chain 1 (Vpreb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108845.1

ORF Insert Sequence:

ATGGCCTGGA CGTCTGCCCT GCTCATACTG CTGGCCCATC TCACAGGTTG TGGCCCTCAG 
CCCGTGCTGC ATCAGCCACC ATCGGCCTCT TCCTTCCTTG GAACCTCCAT CCGCCTCACC 
TGTGCCCTGA GCAGCAACCA TAACATTGGC ATTTACAGCA TTTACTGGTA CCAGCAGAGG 
CCGGGCCACC CTCCCACGTT CCTGCTGAGA TTCTTCTCAC ACTCAGACAA GCTCCAGGGT 
CCCAAGATCC CCCCTCGCTT CTCCGGATCC AAAGATACAG CCAGGAACCT GGGGTACCTG 
AGCATCTCTG ACCTGCAGCC AGAGGACGAG GCTGTGTATT ACTGCGCCGT GGGGCTTCGG 
AGCTGGGAAA AGGAGAAGAG GATGGAGAGG GAGTGGGAAG AAGAAAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Both the pre-BCR and the IL-7Ralpha are essential for expansion at the pre-BII cell stage in vivo.
European journal of immunology35(6)1969-76(2005 Jun)
Erlandsson L,Licence S,Gaspal F,Lane P,Corcoran AE,M?rtensson IL

Gene and alternative splicing annotation with AIR.
Genome research15(1)54-66(2005 Jan)
Florea L,Di Francesco V,Miller J,Turner R,Yao A,Harris M,Walenz B,Mobarry C,Merkulov GV,Charlab R,Dew I,Deng Z,Istrail S,Li P,Sutton G

VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus.
Journal of immunology (Baltimore, Md. : 1950)168(12)6286-93(2002 Jun)
Shimizu T,Mundt C,Licence S,Melchers F,M?rtensson IL

Partial block in B lymphocyte development at the transition into the pre-B cell receptor stage in Vpre-B1-deficient mice.
International immunology11(3)453-60(1999 Mar)
M?rtensson A,Argon Y,Melchers F,Dul JL,M?rtensson IL

The murine VpreB1 and VpreB2 genes both encode a protein of the surrogate light chain and are co-expressed during B cell development.
European journal of immunology26(4)906-13(1996 Apr)
Dul JL,Argon Y,Winkler T,ten Boekel E,Melchers F,M?rtensson IL