Ebag9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ebag9
Entrez Gene ID	299864
Full Name	estrogen receptor binding site associated, antigen, 9
General protein information	Preferred Names estrogen receptor binding site associated, antigen, 9 Names receptor-binding cancer antigen expressed on SiSo cells estrogen receptor-binding fragment-associated gene 9 protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_008765451.2	XP_008763673.1	receptor-binding cancer antigen expressed on SiSo cells isoform X1
NM_001009665.1	NP_001009665.1	receptor-binding cancer antigen expressed on SiSo cells precursor
XM_006241613.3	XP_006241675.1	receptor-binding cancer antigen expressed on SiSo cells isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ebag9	NP_001017088.1
	EBAG9	XP_002805507.1
Bos taurus (cattle)	EBAG9	NP_001071483.1
Homo sapiens (human)	EBAG9	NP_004206.1
Pan troglodytes (chimpanzee)	EBAG9	XP_519909.1
Canis lupus familiaris (dog)	EBAG9	NP_001002996.1
Mus musculus (house mouse)	Ebag9	NP_062353.3
Rattus norvegicus (Norway rat)	Ebag9	NP_001009665.1
Gallus gallus (chicken)	EBAG9	NP_001026120.2
Danio rerio (zebrafish)	ebag9	NP_957388.1

Gene Ontology
Bibliography

Related articles in PubMed

RCAS1 decidual immunoreactivity in severe pre-eclampsia: immune cell presence and activity.
Wicherek L, Basta P, Sikora J, Galazka K, Rytlewski K, Grabiec M, Lazar A, Kalinka J
American journal of reproductive immunology (New York, N.Y. : 1989)58(4)358-66(2007 Oct)

The metallothionein and RCAS1 expression analysis in breast cancer and adjacent tissue regarding the immune cells presence and their activity.
Popiela TJ, Rudnicka-Sosin L, Dutsch-Wicherek M, Klimek M, Basta P, Galazka K, Wicherek L
Neuro endocrinology letters27(6)786-94(2006 Dec)

Clinical significance of RCAS1 as a biomarker of uterine cancer.
Sonoda K, Miyamoto S, Hirakawa T, Yagi H, Yotsumoto F, Nakashima M, Watanabe T, Nakano H
Gynecologic oncology103(3)924-31(2006 Dec)

Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.
Moser K, White FM
Journal of proteome research5(1)98-104(2006 Jan)

Metallothionein and RCAS1 expression in comparison to immunological cells activity in endometriosis, endometrial adenocarcinoma and endometrium according to menstrual cycle changes.
Wicherek L, Popiela TJ, Galazka K, Dutsch-Wicherek M, Op?awski M, Basta A, Klimek M
Gynecologic oncology99(3)622-30(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EBAG9 and Snapin have roles in controlling exocytosis processes
Title: EBAG9 adds a new layer of control on large dense-core vesicle exocytosis via interaction with Snapin.

The following Ebag9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ebag9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00766	NM_001009665.1 Latest version!	Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa00766	XM_006241613.3 Latest version!	Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa45641	XM_008765451.2 Latest version!	Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ebag9 ( NM_001009665.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009665.1 Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), mRNA.

Price & Availability↑

CloneID	ORa00766
Clone ID Related Accession (Same CDS sequence)	NM_001009665.1 , XM_006241613.3
Accession Version	NM_001009665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	receptor-binding cancer antigen expressed on SiSo cells precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC087093.1. On Jan 13, 2005 this sequence version replaced XM_216915.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC087093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCATCA CTCAGTTTCG GTTATTTAAA GTTTGTACCT GCCTAGCAAC AGTGTTGTCG 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGAAGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCCA CCACAGTTGA TTATTCGTCT GTCCCTAAGC AGACAGACGT GGAGGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACAAGT GTGAAGATTG AAGGAGGGAC TGGGAATGCA 
GCAGCGCAGC AGAACTCTTT GGAACAACTG GAGCCTGACT ATTTCAAGGA CATGACACCA 
ACTATACGGA AAACGCAGAA AATTGTCATT AAGAAGAGAG AACCATTGAG TTTTGGTGTC 
CCAGATGGTA GCACAGGTTT TTCCAGTAGA TTGGCAGCTA CACAAGATAT GCCTTTTATT 
CATCAGTCTT CAGAATTAGG TGACTTAGAT ACATGGCAAG AAAATAGCAA TGCATGGGAA 
GAGGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GGCAGCAGAA AATTGCAGAC 
AGAGAGAAGA GAGCAGCAGA ACAACAAAGG AAGAAAATGG AGAAGGAAGC ACAGCGTCTG 
CTGAAGAAGG AACAAAACAA AATGGGTGTG AAGCTTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009665.1
CDS	211..852
Translation	MAITQFRLFKVCTCLATVLSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGTGNAAAQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLSFGVPDGSTGFSSRLAATQDMPFIHQSSELGDLDTWQENSNAWEEEEDAAWQAEEVLRQQKIADREKRAAEQQRKKMEKEAQRLLKKEQNKMGVKLS

Target ORF information:

RefSeq Version	NM_001009665.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009665.1

ORF Insert Sequence:

ATGGCCATCA CTCAGTTTCG GTTATTTAAA GTTTGTACCT GCCTAGCAAC AGTGTTGTCG 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGAAGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCCA CCACAGTTGA TTATTCGTCT GTCCCTAAGC AGACAGACGT GGAGGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACAAGT GTGAAGATTG AAGGAGGGAC TGGGAATGCA 
GCAGCGCAGC AGAACTCTTT GGAACAACTG GAGCCTGACT ATTTCAAGGA CATGACACCA 
ACTATACGGA AAACGCAGAA AATTGTCATT AAGAAGAGAG AACCATTGAG TTTTGGTGTC 
CCAGATGGTA GCACAGGTTT TTCCAGTAGA TTGGCAGCTA CACAAGATAT GCCTTTTATT 
CATCAGTCTT CAGAATTAGG TGACTTAGAT ACATGGCAAG AAAATAGCAA TGCATGGGAA 
GAGGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GGCAGCAGAA AATTGCAGAC 
AGAGAGAAGA GAGCAGCAGA ACAACAAAGG AAGAAAATGG AGAAGGAAGC ACAGCGTCTG 
CTGAAGAAGG AACAAAACAA AATGGGTGTG AAGCTTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ebag9 ( XM_006241613.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241675.1 Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa00766
Clone ID Related Accession (Same CDS sequence)	NM_001009665.1 , XM_006241613.3
Accession Version	XM_006241613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	receptor-binding cancer antigen expressed on SiSo cells isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241613.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATCA CTCAGTTTCG GTTATTTAAA GTTTGTACCT GCCTAGCAAC AGTGTTGTCG 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGAAGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCCA CCACAGTTGA TTATTCGTCT GTCCCTAAGC AGACAGACGT GGAGGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACAAGT GTGAAGATTG AAGGAGGGAC TGGGAATGCA 
GCAGCGCAGC AGAACTCTTT GGAACAACTG GAGCCTGACT ATTTCAAGGA CATGACACCA 
ACTATACGGA AAACGCAGAA AATTGTCATT AAGAAGAGAG AACCATTGAG TTTTGGTGTC 
CCAGATGGTA GCACAGGTTT TTCCAGTAGA TTGGCAGCTA CACAAGATAT GCCTTTTATT 
CATCAGTCTT CAGAATTAGG TGACTTAGAT ACATGGCAAG AAAATAGCAA TGCATGGGAA 
GAGGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GGCAGCAGAA AATTGCAGAC 
AGAGAGAAGA GAGCAGCAGA ACAACAAAGG AAGAAAATGG AGAAGGAAGC ACAGCGTCTG 
CTGAAGAAGG AACAAAACAA AATGGGTGTG AAGCTTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241675.1
CDS	565..1206
Translation	MAITQFRLFKVCTCLATVLSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGTGNAAAQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLSFGVPDGSTGFSSRLAATQDMPFIHQSSELGDLDTWQENSNAWEEEEDAAWQAEEVLRQQKIADREKRAAEQQRKKMEKEAQRLLKKEQNKMGVKLS

Target ORF information:

RefSeq Version	XM_006241613.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241613.3

ORF Insert Sequence:

ATGGCCATCA CTCAGTTTCG GTTATTTAAA GTTTGTACCT GCCTAGCAAC AGTGTTGTCG 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGAAGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCCA CCACAGTTGA TTATTCGTCT GTCCCTAAGC AGACAGACGT GGAGGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACAAGT GTGAAGATTG AAGGAGGGAC TGGGAATGCA 
GCAGCGCAGC AGAACTCTTT GGAACAACTG GAGCCTGACT ATTTCAAGGA CATGACACCA 
ACTATACGGA AAACGCAGAA AATTGTCATT AAGAAGAGAG AACCATTGAG TTTTGGTGTC 
CCAGATGGTA GCACAGGTTT TTCCAGTAGA TTGGCAGCTA CACAAGATAT GCCTTTTATT 
CATCAGTCTT CAGAATTAGG TGACTTAGAT ACATGGCAAG AAAATAGCAA TGCATGGGAA 
GAGGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GGCAGCAGAA AATTGCAGAC 
AGAGAGAAGA GAGCAGCAGA ACAACAAAGG AAGAAAATGG AGAAGGAAGC ACAGCGTCTG 
CTGAAGAAGG AACAAAACAA AATGGGTGTG AAGCTTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ebag9 ( XM_008765451.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763673.1 Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45641
Clone ID Related Accession (Same CDS sequence)	XM_008765451.2
Accession Version	XM_008765451.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	receptor-binding cancer antigen expressed on SiSo cells isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTAA TACATTGTTT CTGTGTGCGT GTGCCTTCCC CACAGGTTCT GGTTTTCACC 
ATGGCCATCA CTCAGTTTCG GTTATTTAAA GTTTGTACCT GCCTAGCAAC AGTGTTGTCG 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGAAGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCCA CCACAGTTGA TTATTCGTCT GTCCCTAAGC AGACAGACGT GGAGGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACAAGT GTGAAGATTG AAGGAGGGAC TGGGAATGCA 
GCAGCGCAGC AGAACTCTTT GGAACAACTG GAGCCTGACT ATTTCAAGGA CATGACACCA 
ACTATACGGA AAACGCAGAA AATTGTCATT AAGAAGAGAG AACCATTGAG TTTTGGTGTC 
CCAGATGGTA GCACAGGTTT TTCCAGTAGA TTGGCAGCTA CACAAGATAT GCCTTTTATT 
CATCAGTCTT CAGAATTAGG TGACTTAGAT ACATGGCAAG AAAATAGCAA TGCATGGGAA 
GAGGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GGCAGCAGAA AATTGCAGAC 
AGAGAGAAGA GAGCAGCAGA ACAACAAAGG AAGAAAATGG AGAAGGAAGC ACAGCGTCTG 
CTGAAGAAGG AACAAAACAA AATGGGTGTG AAGCTTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763673.1
CDS	232..933
Translation	MCLIHCFCVRVPSPQVLVFTMAITQFRLFKVCTCLATVLSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGTGNAAAQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLSFGVPDGSTGFSSRLAATQDMPFIHQSSELGDLDTWQENSNAWEEEEDAAWQAEEVLRQQKIADREKRAAEQQRKKMEKEAQRLLKKEQNKMGVKLS

Target ORF information:

RefSeq Version	XM_008765451.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus estrogen receptor binding site associated, antigen, 9 (Ebag9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765451.2

ORF Insert Sequence:

ATGTGTCTAA TACATTGTTT CTGTGTGCGT GTGCCTTCCC CACAGGTTCT GGTTTTCACC 
ATGGCCATCA CTCAGTTTCG GTTATTTAAA GTTTGTACCT GCCTAGCAAC AGTGTTGTCG 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGAAGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCCA CCACAGTTGA TTATTCGTCT GTCCCTAAGC AGACAGACGT GGAGGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACAAGT GTGAAGATTG AAGGAGGGAC TGGGAATGCA 
GCAGCGCAGC AGAACTCTTT GGAACAACTG GAGCCTGACT ATTTCAAGGA CATGACACCA 
ACTATACGGA AAACGCAGAA AATTGTCATT AAGAAGAGAG AACCATTGAG TTTTGGTGTC 
CCAGATGGTA GCACAGGTTT TTCCAGTAGA TTGGCAGCTA CACAAGATAT GCCTTTTATT 
CATCAGTCTT CAGAATTAGG TGACTTAGAT ACATGGCAAG AAAATAGCAA TGCATGGGAA 
GAGGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GGCAGCAGAA AATTGCAGAC 
AGAGAGAAGA GAGCAGCAGA ACAACAAAGG AAGAAAATGG AGAAGGAAGC ACAGCGTCTG 
CTGAAGAAGG AACAAAACAA AATGGGTGTG AAGCTTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RCAS1 decidual immunoreactivity in severe pre-eclampsia: immune cell presence and activity.
American journal of reproductive immunology (New York, N.Y. : 1989)58(4)358-66(2007 Oct)
Wicherek L,Basta P,Sikora J,Galazka K,Rytlewski K,Grabiec M,Lazar A,Kalinka J

The metallothionein and RCAS1 expression analysis in breast cancer and adjacent tissue regarding the immune cells presence and their activity.
Neuro endocrinology letters27(6)786-94(2006 Dec)
Popiela TJ,Rudnicka-Sosin L,Dutsch-Wicherek M,Klimek M,Basta P,Galazka K,Wicherek L

Clinical significance of RCAS1 as a biomarker of uterine cancer.
Gynecologic oncology103(3)924-31(2006 Dec)
Sonoda K,Miyamoto S,Hirakawa T,Yagi H,Yotsumoto F,Nakashima M,Watanabe T,Nakano H

Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.
Journal of proteome research5(1)98-104(2006 Jan)
Moser K,White FM

Metallothionein and RCAS1 expression in comparison to immunological cells activity in endometriosis, endometrial adenocarcinoma and endometrium according to menstrual cycle changes.
Gynecologic oncology99(3)622-30(2005 Dec)
Wicherek L,Popiela TJ,Galazka K,Dutsch-Wicherek M,Op?awski M,Basta A,Klimek M