Nts cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nts
Entrez Gene ID	299757
Full Name	neurotensin
General protein information	Preferred Names neurotensin Names neurotensin/neuromedin N neuromedin N
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q21
Summary	gene encodes a precursor protein for both peptides; neurotensin may be involved in smooth muscle contraction and possibly fat metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102381.1	NP_001095851.1	neurotensin/neuromedin N precursor

Pathways from BioSystems

Homologies

	NTS	XP_001090068.1
Mus musculus (house mouse)	Nts	NP_077755.1
Homo sapiens (human)	NTS	NP_006174.1
Pan troglodytes (chimpanzee)	NTS	XP_001165130.1
Canis lupus familiaris (dog)	NTS	XP_854478.1
Gallus gallus (chicken)	NTS	NP_001264289.1
Rattus norvegicus (Norway rat)	Nts	NP_001095851.1
Bos taurus (cattle)	NTS	NP_776370.2
Xenopus tropicalis (tropical clawed frog)	nts	XP_002937110.1

Gene Ontology
Bibliography

Related articles in PubMed

Neurotensin in the nucleus accumbens reverses dopamine supersensitivity evoked by antipsychotic treatment.
Servonnet A, Minogianis EA, Bouchard C, B?dard AM, L?vesque D, Rompr? PP, Samaha AN
Neuropharmacology12310-21(2017 Sep)

Repeated ventral midbrain neurotensin injections sensitize to amphetamine-induced locomotion and ERK activation: A role for NMDA receptors.
Voyer D, L?vesque D, Rompr? PP
Neuropharmacology112(Pt A)150-163(2017 Jan)

Gene-environment interactions in a rat model of depression. Maternal separation affects neurotensin in selected brain regions.
Ellenbroek BA, Angelucci F, Husum H, Math? AA
Neuropeptides5983-88(2016 Oct)

Role of context in neurotensin-induced sensitization to the locomotor stimulant effect of amphetamine.
Rouibi K, Rompr? PP
Peptides58103-7(2014 Aug)

Characterization of neurotensin receptors.
Kitabgi P, P?laprat D
Current protocols in pharmacologyChapter 1Unit 1.29(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results support the hypothesis that neurotensin is stimulating glutamate neurotransmission to initiate neural changes that sub-serve amphetamine sensitization and that glutamate is acting on NMDA receptors that are mostly likely composed of GluN2A, but not GluN2B, subunits.
Title: Repeated ventral midbrain neurotensin injections sensitize to amphetamine-induced locomotion and ERK activation: A role for NMDA receptors.

The present study revealed baseline regional differences in neurotensin-like immunoreactivity concentrations between the FSL and FRL rat strains and demonstrated that early maternal deprivation differentially affects the two strains.
Title: Gene-environment interactions in a rat model of depression. Maternal separation affects neurotensin in selected brain regions.

This context-dependency was not found however for amphetamine-induced non-ambulatory and vertical activity suggesting that neurotensin can induce both a context-dependent and context-independent sensitization.
Title: Role of context in neurotensin-induced sensitization to the locomotor stimulant effect of amphetamine.

neurotensin plays an important role in the regulation of spinal cardiovascular function, affecting both tone and adaptive reflexes
Title: Intrathecal neurotensin is hypotensive, sympathoinhibitory and enhances the baroreflex in anaesthetized rat.

PREP is not significantly responsible for the in vivo extracellular space cleavage of substance P or neurotensin.
Title: Inhibition of prolyl oligopeptidase by KYP-2047 fails to increase the extracellular neurotensin and substance P levels in rat striatum.

The following Nts gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nts cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02203	NM_001102381.1 Latest version!	Rattus norvegicus neurotensin (Nts), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nts ( NM_001102381.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001095851.1 Rattus norvegicus neurotensin (Nts), mRNA.

Price & Availability↑

CloneID	ORa02203
Clone ID Related Accession (Same CDS sequence)	NM_001102381.1
Accession Version	NM_001102381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	neurotensin/neuromedin N precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473960.2. On or before Aug 22, 2007 this sequence version replaced XM_216884.4, XM_001080677.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ214250.1, FM072651.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATAGGAA TGAACCTTCA GCTGGTGTGC CTGACTCTCC TGGCTTTCAG CTCCTGGAGT 
CTGTGCTCAG ATTCAGAAGA AGATGTGAGA GTCCTGGAGG CAGATCTCCT GACAAATATG 
CATGCATCGA AGGTCAGCAA AGGAAGTCCT CCCTCTTGGA AAATGACCTT GCTCAACGTT 
TGCAGCCTGA TCAATAACCT GAACAGCGCA GCGGAGGAAG CGGGAGAAAT GCGTGATGAC 
GACCTTGTTG CGAAAAGGAA ACTTCCCCTT GTCCTGGATG ACTTTAGCTT GGAAGCGCTG 
CTGACCGTAT TCCAACTCCA GAAGATCTGC CGCAGCCGGG CCTTTCAACA CTGGGAGATA 
ATTCAGGAGG ATATCCTCGA TCATGGCAAT GAGAAAACTG AGAAGGAAGA AGTGATAAAG 
AGAAAAATCC CTTATATTCT GAAACGGCAG CTCTATGAGA ATAAACCCAG AAGGCCCTAC 
ATACTCAAGA GGGCCTCCTA CTACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095851.1
CDS	81..590
Translation	MIGMNLQLVCLTLLAFSSWSLCSDSEEDVRVLEADLLTNMHASKVSKGSPPSWKMTLLNVCSLINNLNSAAEEAGEMRDDDLVAKRKLPLVLDDFSLEALLTVFQLQKICRSRAFQHWEIIQEDILDHGNEKTEKEEVIKRKIPYILKRQLYENKPRRPYILKRASYYY

Target ORF information:

RefSeq Version	NM_001102381.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurotensin (Nts), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102381.1

ORF Insert Sequence:

ATGATAGGAA TGAACCTTCA GCTGGTGTGC CTGACTCTCC TGGCTTTCAG CTCCTGGAGT 
CTGTGCTCAG ATTCAGAAGA AGATGTGAGA GTCCTGGAGG CAGATCTCCT GACAAATATG 
CATGCATCGA AGGTCAGCAA AGGAAGTCCT CCCTCTTGGA AAATGACCTT GCTCAACGTT 
TGCAGCCTGA TCAATAACCT GAACAGCGCA GCGGAGGAAG CGGGAGAAAT GCGTGATGAC 
GACCTTGTTG CGAAAAGGAA ACTTCCCCTT GTCCTGGATG ACTTTAGCTT GGAAGCGCTG 
CTGACCGTAT TCCAACTCCA GAAGATCTGC CGCAGCCGGG CCTTTCAACA CTGGGAGATA 
ATTCAGGAGG ATATCCTCGA TCATGGCAAT GAGAAAACTG AGAAGGAAGA AGTGATAAAG 
AGAAAAATCC CTTATATTCT GAAACGGCAG CTCTATGAGA ATAAACCCAG AAGGCCCTAC 
ATACTCAAGA GGGCCTCCTA CTACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neurotensin in the nucleus accumbens reverses dopamine supersensitivity evoked by antipsychotic treatment.
Neuropharmacology12310-21(2017 Sep)
Servonnet A,Minogianis EA,Bouchard C,B?dard AM,L?vesque D,Rompr? PP,Samaha AN

Repeated ventral midbrain neurotensin injections sensitize to amphetamine-induced locomotion and ERK activation: A role for NMDA receptors.
Neuropharmacology112(Pt A)150-163(2017 Jan)
Voyer D,L?vesque D,Rompr? PP

Gene-environment interactions in a rat model of depression. Maternal separation affects neurotensin in selected brain regions.
Neuropeptides5983-88(2016 Oct)
Ellenbroek BA,Angelucci F,Husum H,Math? AA

Role of context in neurotensin-induced sensitization to the locomotor stimulant effect of amphetamine.
Peptides58103-7(2014 Aug)
Rouibi K,Rompr? PP

Characterization of neurotensin receptors.
Current protocols in pharmacologyChapter 1Unit 1.29(2004 May)
Kitabgi P,P?laprat D