Adi1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adi1
Entrez Gene ID	298934
Full Name	acireductone dioxygenase 1
Synonyms	Alp1,Fe-ARD,MTCBP-1,Ni-ARD
General protein information	Preferred Names acireductone dioxygenase 1 Names 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase ARD aci-reductone dioxygenase (ARD)-like protein 1 acireductone dioxygenase (Fe(2+)-requiring) acireductone dioxygenase (Ni(2+)-requiring) androgen-responsive ARD-like protein 1 androgen-responsive gene encoding an ARD-like protein membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q16
Summary	may be involved in the methionine salvage pathway and prostate homeostasis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199097.1	NP_954528.1	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	ARD1	NP_567443.1
Oryza sativa (rice)	Os10g0419400	NP_001064618.1
Homo sapiens (human)	ADI1	NP_060739.2
Canis lupus familiaris (dog)	ADI1	XP_005630647.1
Bos taurus (cattle)	ADI1	NP_001030545.1
Mus musculus (house mouse)	Adi1	NP_598813.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005618	XP_315627.3
Canis lupus familiaris (dog)	LOC100856577	XP_003640360.1
Rattus norvegicus (Norway rat)	Adi1	NP_954528.1
Danio rerio (zebrafish)	adi1	NP_955962.1
Drosophila melanogaster (fruit fly)	CG32068	NP_001097577.1
Xenopus tropicalis (tropical clawed frog)	adi1	NP_001004933.1
	LOC722050	XP_001118248.1
Gallus gallus (chicken)	ADI1	NP_001026260.1
Pan troglodytes (chimpanzee)	ADI1	XP_001153347.1
Arabidopsis thaliana (thale cress)	ATARD2	NP_001031640.2
Arabidopsis thaliana (thale cress)	ARD3	NP_180208.1
Oryza sativa (rice)	Os03g0161800	NP_001049046.1

Gene Ontology
Bibliography

Related articles in PubMed

Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway.
Hirano W, Gotoh I, Uekita T, Seiki M
Genes to cells : devoted to molecular & cellular mechanisms10(6)565-74(2005 Jun)

Identification and characterization of an androgen-responsive gene encoding an aci-reductone dioxygenase-like protein in the rat prostate.
Oram S, Jiang F, Cai X, Haleem R, Dincer Z, Wang Z
Endocrinology145(4)1933-42(2004 Apr)

Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors.
Uekita T, Gotoh I, Kinoshita T, Itoh Y, Sato H, Shiomi T, Okada Y, Seiki M
The Journal of biological chemistry279(13)12734-43(2004 Mar)

Identification of a hepatic factor capable of supporting hepatitis C virus replication in a nonpermissive cell line.
Yeh CT, Lai HY, Chen TC, Chu CM, Liaw YF
Journal of virology75(22)11017-24(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

May have an important role in androgen regulated prostate homeostasis as well as in prostate cancer progression by regulating cell death of prostate cancer cells.
Title: Identification and characterization of an androgen-responsive gene encoding an aci-reductone dioxygenase-like protein in the rat prostate.

The following Adi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04678	NM_199097.1 Latest version!	Rattus norvegicus acireductone dioxygenase 1 (Adi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adi1 ( NM_199097.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954528.1 Rattus norvegicus acireductone dioxygenase 1 (Adi1), mRNA.

Price & Availability↑

CloneID	ORa04678
Clone ID Related Accession (Same CDS sequence)	NM_199097.1
Accession Version	NM_199097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-08
Organism	Rattus norvegicus(Norway rat)
Product	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY346335.1. On Jan 1, 2004 this sequence version replaced XM_216669.2. ##Evidence-Data-START## Transcript exon combination :: AY346335.1, FQ219675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCAGG CCTGGTATAT GGACGAGTCC ACGGCCGACC CGCGGATGCC CCACCGCGCA 
CAGCCCGACC GCCCTGTGGG ACTGGAGCAG CTGCGCACGC TCGGAGTGCT CTATTGGAAG 
CTAGATGCGG ACAAGTATGA GAATGATCCA GAACTGGAAC AGATTCGGAA AACAAGGAAC 
TACTCATGGA TGGATATCAT CACCATATGC AAAGATTCAC TTCCCAATTA TGAGGAGAAG 
ATCAAGATGT TCTTTGAGGA ACATCTGCAT CTGGATGAGG AGATCCGCTA CATCCTGGAG 
GGCAGCGGGT ACTTCGATGT CAGGGACAAG GAGGACAAGT GGATCCGGAT TTCTATGGAG 
AAGGGGGACA TGATCACGCT TCCTGCCGGC ATTTATCACC GCTTCACGCT GGACGAGAAG 
AACTACGTGA AGGCCATGCG GCTGTTTGTT GGAGAACCTG TATGGACACC ATACAACCGG 
GCAGCTGAVC ATTTTGATGC CCGGGTACAG TACGTGAAGT TTTTGGAAGG AACAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954528.1
CDS	17..556
Translation	MVQAWYMDESTADPRMPHRAQPDRPVGLEQLRTLGVLYWKLDADKYENDPELEQIRKTRNYSWMDIITICKDSLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDVRDKEDKWIRISMEKGDMITLPAGIYHRFTLDEKNYVKAMRLFVGEPVWTPYNRAAnullHFDARVQYVKFLEGTA

Target ORF information:

RefSeq Version	NM_199097.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acireductone dioxygenase 1 (Adi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199097.1

ORF Insert Sequence:

ATGGTGCAGG CCTGGTATAT GGACGAGTCC ACGGCCGACC CGCGGATGCC CCACCGCGCA 
CAGCCCGACC GCCCTGTGGG ACTGGAGCAG CTGCGCACGC TCGGAGTGCT CTATTGGAAG 
CTAGATGCGG ACAAGTATGA GAATGATCCA GAACTGGAAC AGATTCGGAA AACAAGGAAC 
TACTCATGGA TGGATATCAT CACCATATGC AAAGATTCAC TTCCCAATTA TGAGGAGAAG 
ATCAAGATGT TCTTTGAGGA ACATCTGCAT CTGGATGAGG AGATCCGCTA CATCCTGGAG 
GGCAGCGGGT ACTTCGATGT CAGGGACAAG GAGGACAAGT GGATCCGGAT TTCTATGGAG 
AAGGGGGACA TGATCACGCT TCCTGCCGGC ATTTATCACC GCTTCACGCT GGACGAGAAG 
AACTACGTGA AGGCCATGCG GCTGTTTGTT GGAGAACCTG TATGGACACC ATACAACCGG 
GCAGCTGAVC ATTTTGATGC CCGGGTACAG TACGTGAAGT TTTTGGAAGG AACAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway.
Genes to cells : devoted to molecular & cellular mechanisms10(6)565-74(2005 Jun)
Hirano W,Gotoh I,Uekita T,Seiki M

Identification and characterization of an androgen-responsive gene encoding an aci-reductone dioxygenase-like protein in the rat prostate.
Endocrinology145(4)1933-42(2004 Apr)
Oram S,Jiang F,Cai X,Haleem R,Dincer Z,Wang Z

Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors.
The Journal of biological chemistry279(13)12734-43(2004 Mar)
Uekita T,Gotoh I,Kinoshita T,Itoh Y,Sato H,Shiomi T,Okada Y,Seiki M

Identification of a hepatic factor capable of supporting hepatitis C virus replication in a nonpermissive cell line.
Journal of virology75(22)11017-24(2001 Nov)
Yeh CT,Lai HY,Chen TC,Chu CM,Liaw YF