Gen1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gen1
Entrez Gene ID	298884
Full Name	GEN1 Holliday junction 5' flap endonuclease
Synonyms	RGD1559792
General protein information	Preferred Names GEN1 Holliday junction 5' flap endonuclease Names flap endonuclease GEN homolog 1 Gen endonuclease homolog 1 Gen homolog 1, endonuclease
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q15

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106717.1	NP_001100187.1	flap endonuclease GEN homolog 1
XM_006239896.2	XP_006239958.1	flap endonuclease GEN homolog 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GEN1	NP_872431.3
Bos taurus (cattle)	GEN1	NP_001192379.1
Gallus gallus (chicken)	GEN1	XP_004935834.1
Pan troglodytes (chimpanzee)	GEN1	XP_003949810.1
	GEN1	XP_001092423.1
Mus musculus (house mouse)	Gen1	NP_796305.3
Canis lupus familiaris (dog)	GEN1	XP_540093.2
Rattus norvegicus (Norway rat)	Gen1	NP_001100187.1
Arabidopsis thaliana (thale cress)	AT1G01880	NP_171691.2

Gene Ontology
Bibliography

Related articles in PubMed

Resolution of single and double Holliday junction recombination intermediates by GEN1.
Shah Punatar R, Martin MJ, Wyatt HD, Chan YW, West SC
Proceedings of the National Academy of Sciences of the United States of America114(3)443-450(2017 Jan)

GEN1 promotes Holliday junction resolution by a coordinated nick and counter-nick mechanism.
Chan YW, West S
Nucleic acids research43(22)10882-92(2015 Dec)

Human Holliday junction resolvase GEN1 uses a chromodomain for efficient DNA recognition and cleavage.
Lee SH, Princz LN, Kl?gel MF, Habermann B, Pfander B, Biert?mpfel C
eLife4(2015 Dec)

Human GEN1 and the SLX4-associated nucleases MUS81 and SLX1 are essential for the resolution of replication-induced Holliday junctions.
Garner E, Kim Y, Lach FP, Kottemann MC, Smogorzewska A
Cell reports5(1)207-15(2013 Oct)

A novel role of human holliday junction resolvase GEN1 in the maintenance of centrosome integrity.
Gao M, Rendtlew Danielsen J, Wei LZ, Zhou DP, Xu Q, Li MM, Wang ZQ, Tong WM, Yang YG
PloS one7(11)e49687(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gen1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gen1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02047	NM_001106717.1 Latest version!	Rattus norvegicus GEN1 Holliday junction 5' flap endonuclease (Gen1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa29211	XM_006239896.2 Latest version!	Rattus norvegicus GEN1 Holliday junction 5' flap endonuclease (Gen1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gen1 ( NM_001106717.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100187.1 Rattus norvegicus GEN1 Holliday junction 5' flap endonuclease (Gen1), mRNA.

Price & Availability↑

CloneID	ORa02047
Clone ID Related Accession (Same CDS sequence)	NM_001106717.1
Accession Version	NM_001106717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	flap endonuclease GEN homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473947.1. On or before Oct 4, 2007 this sequence version replaced XM_233966.4, XM_001072749.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGAGTGA ATGACCTGTG GCAAATTTTG GAGCCTGTAA AGCAACATAT CCACTTGCAG 
GATCTTTGTG GGAAGACCAT TGCCGTTGAT TTGAGTCTCT GGGTATGCGA GGCACAGACA 
GTGAAGAAAA TGATTGGAAC TGTCATGAAG CCCCACCTCA GGAACCTATT TTTTCGTATT 
TCATATTTAA CTCAAATGAA TGTAAAACTG GTGTTTGTGA TGGAAGGGGA GCCACCAAAG 
CTGAAAGCTG ATGTCATGAA CAAGAGGACT CAGACTCGAT ACGGGCCTTC TGGAAAATCG 
AGGTCTCAGA AAACAGGGAG ATCGCATTTT AAGTCAGTCT TACGAGAGTG CCTTGAAATG 
CTAGAGTGCC TAGGAATGCC CTGGGTCCAG GCTGCGGGGG AAGCTGAGGC CATGTGTGCT 
TACCTTAATG CCAGCGGCCA TGTGGACGGC TGCCTCACCA ACGACGGCGA TGCTTTCCTT 
TATGGAGCCC AGACTGTGTA CAGGAATTTC ACCATGAACA CAAAGGATCC ACATGTTGAC 
TGTTACACGG TATCATCTAT CAAGAGTAAG CTAGGCTTGG ATCGAGATGC TCTGGTGGGA 
CTGGCGGTAC TTCTTGGTTG TGATTACCTT CCGAAGGGAG TGCCTGGAGT TGGAAAAGAA 
CAAGCGTTAA AACTTTTGCA GATTTTGAAA GGTCAAAGTT TGCTTCAGAG GTTTAACCAG 
TGGACTGAAG AACCTGGCTA CTCTGTTCCA CAATCAGCAG CAAAAAAAGT GGTTCACTGC 
TCGGTGTGTT CCCATCCAGG TTCTCCTAAG GATCATGAAC GCAATGGGTG CACACTATGT 
AAAAGTGATA GATACTGTGA ACCACATGAT TATGAATACC TCTGTCCTTG TGAATGGCAC 
CAGACAGACC ATAATAGGCA ACTAAATGAA ATAGAAAACA ATATTAAAAA GAAAGCTTGC 
AGTTGCGAAG GATTTCCATT CCATGAGGTC ATCCAAGAAT TCCTTTCGAA TAAGAATAAA 
CTGTTAAAAC CGATCAGGTA CCAAAGACCT GATTTATTAT TGTTTCAGAG ATTTACTGTT 
CAAAAAATGG AGTGGCCCAG TCACTATGCG TGTGAAAAAT TGTTGGTGCT TTTGACCCGC 
TATGACATGA TAGAAAGAAA ACTTGGTAGA AAGACTTCCA ATCAACTACA GCCAATTAGA 
ATTGTTAAAC CTCGAATCAG AAATGGAGTC TGTTGTCTTG AAATAGAATG GGAAAAGCCT 
GAACATTATT CGGTAGAAGA TACACAACCT GGAGGACTGG ATCTCCTTAC AATCGAAGAA 
GCATCACTGT TTGAAGCAGC CTATCCTGAA GTTGTAGCTA TCTACCAGAA ACAACAGTCA 
GAAACTAAAG GGAAGAAACA GAAAAATATA AAAATTAAGC CCAAAGGAAG CCATCTACCA 
GAATCAGATG ATGTGATCAG TTCCCAGTCA CTTATTACTT TAGAACCTAT ATCAAAAGCC 
TTCCCCAAGC AGAATCCCAA ATTTGATTTG GAAATTTCTC CAGATCCTAT TTTAGCACAG 
GAGTCTACTT CTCCCTCATT GAGTAGTTTT GTTTCCCCTG AAAATGCTCT TGGTTTGAAT 
TTGCAAGAAC AGTTAATGCC TTCTCCAAGA ACTTTGGCTG TAAAGCAAAC TAAAGATGTC 
AGTAATTTTC TAGTTTCAGA GTGTAGTCAG CCCAGTTCTT CATCCCATAA TATTTCTGTG 
ATTACTGATC TACACTTGAG TACCATTGAC TGGGCAGGTA CTTCTTTTAG TAATTCTCCA 
GCAGTTCAAA GAAACACTTT CTCTCAAGAT TTACAATCAG AACCTGAATC ATCAGCCATC 
CTTCCTGCCT TTGAACAGTT GTCATATGAA TCAGAACAAT GTACCTTAAG CAACAAAGTG 
TCCAATAGAG GTCTTCAGAA GAGTGGTCCT GAAGAGCATC TCCTTTCCAG CATCACTGGT 
TTACATCTTC ACGATTTGCC TCTAAAGGAA AGAATACATA TAAAATCTTC ATGCCCTCAG 
TATAATGTAG GAGCAGATGC TGACCTGGAA AGTTTACCCC TAACAATGAA TCACTCTTGC 
ATTGCTTACA GTAGCTCTGG TGGTTCATCC CATTTTTCGA AGGATCTTAC AGGGGTATAT 
CTTCAGAAAG AGCTCAGAAA CTCTAAAGTT CTAGACAGCC ACTTCCTTCA AGAAAACTTC 
AGGGTGAATA CTTCCTTACC TTATTCTTTC AATGACGAAG CAGTAAAGAC TTCCAGTCTT 
CAAGTTGGGC TGCCAACTGC TGCTGTACCT CATAATCCAA AAGTTGATGT GAAAACTAAG 
AACCTTGTCA AGAAGAGTAG TGTTTGCCTC AAGAGAGATT CCTCAGATGA AGAGAATGCT 
GCAGTGTCCT GGAAAGCTAA GTATACTGCT CCTAAATTGA AACACAGCTC TCATAATCAT 
AGCCTAGTCC ATGACAGAGA CAGTACTCAC AACAAACACA GAAATCCGAA AGATGAATCT 
AACGAAACCA AACTGTGTAC TGAGTCTTTT AAAATAGCTG AAGATGAAGA AAACAAGTTC 
TCAGATCCAG CAAGAAATCC CCAGAGTTTT CTACAATGTC ATAACAAGGA TGAGGACTCT 
GCTGACTGCT GGGAAAGTCC TCTCCCTTTA CGCCAGAGGT TAAAGCTCAG GTTCCAGAAC 
ACTCAAAGTA GACTTTATAA TACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100187.1
CDS	244..2970
Translation	MGVNDLWQILEPVKQHIHLQDLCGKTIAVDLSLWVCEAQTVKKMIGTVMKPHLRNLFFRISYLTQMNVKLVFVMEGEPPKLKADVMNKRTQTRYGPSGKSRSQKTGRSHFKSVLRECLEMLECLGMPWVQAAGEAEAMCAYLNASGHVDGCLTNDGDAFLYGAQTVYRNFTMNTKDPHVDCYTVSSIKSKLGLDRDALVGLAVLLGCDYLPKGVPGVGKEQALKLLQILKGQSLLQRFNQWTEEPGYSVPQSAAKKVVHCSVCSHPGSPKDHERNGCTLCKSDRYCEPHDYEYLCPCEWHQTDHNRQLNEIENNIKKKACSCEGFPFHEVIQEFLSNKNKLLKPIRYQRPDLLLFQRFTVQKMEWPSHYACEKLLVLLTRYDMIERKLGRKTSNQLQPIRIVKPRIRNGVCCLEIEWEKPEHYSVEDTQPGGLDLLTIEEASLFEAAYPEVVAIYQKQQSETKGKKQKNIKIKPKGSHLPESDDVISSQSLITLEPISKAFPKQNPKFDLEISPDPILAQESTSPSLSSFVSPENALGLNLQEQLMPSPRTLAVKQTKDVSNFLVSECSQPSSSSHNISVITDLHLSTIDWAGTSFSNSPAVQRNTFSQDLQSEPESSAILPAFEQLSYESEQCTLSNKVSNRGLQKSGPEEHLLSSITGLHLHDLPLKERIHIKSSCPQYNVGADADLESLPLTMNHSCIAYSSSGGSSHFSKDLTGVYLQKELRNSKVLDSHFLQENFRVNTSLPYSFNDEAVKTSSLQVGLPTAAVPHNPKVDVKTKNLVKKSSVCLKRDSSDEENAAVSWKAKYTAPKLKHSSHNHSLVHDRDSTHNKHRNPKDESNETKLCTESFKIAEDEENKFSDPARNPQSFLQCHNKDEDSADCWESPLPLRQRLKLRFQNTQSRLYNT

Target ORF information:

RefSeq Version	NM_001106717.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GEN1 Holliday junction 5' flap endonuclease (Gen1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106717.1

ORF Insert Sequence:

ATGGGAGTGA ATGACCTGTG GCAAATTTTG GAGCCTGTAA AGCAACATAT CCACTTGCAG 
GATCTTTGTG GGAAGACCAT TGCCGTTGAT TTGAGTCTCT GGGTATGCGA GGCACAGACA 
GTGAAGAAAA TGATTGGAAC TGTCATGAAG CCCCACCTCA GGAACCTATT TTTTCGTATT 
TCATATTTAA CTCAAATGAA TGTAAAACTG GTGTTTGTGA TGGAAGGGGA GCCACCAAAG 
CTGAAAGCTG ATGTCATGAA CAAGAGGACT CAGACTCGAT ACGGGCCTTC TGGAAAATCG 
AGGTCTCAGA AAACAGGGAG ATCGCATTTT AAGTCAGTCT TACGAGAGTG CCTTGAAATG 
CTAGAGTGCC TAGGAATGCC CTGGGTCCAG GCTGCGGGGG AAGCTGAGGC CATGTGTGCT 
TACCTTAATG CCAGCGGCCA TGTGGACGGC TGCCTCACCA ACGACGGCGA TGCTTTCCTT 
TATGGAGCCC AGACTGTGTA CAGGAATTTC ACCATGAACA CAAAGGATCC ACATGTTGAC 
TGTTACACGG TATCATCTAT CAAGAGTAAG CTAGGCTTGG ATCGAGATGC TCTGGTGGGA 
CTGGCGGTAC TTCTTGGTTG TGATTACCTT CCGAAGGGAG TGCCTGGAGT TGGAAAAGAA 
CAAGCGTTAA AACTTTTGCA GATTTTGAAA GGTCAAAGTT TGCTTCAGAG GTTTAACCAG 
TGGACTGAAG AACCTGGCTA CTCTGTTCCA CAATCAGCAG CAAAAAAAGT GGTTCACTGC 
TCGGTGTGTT CCCATCCAGG TTCTCCTAAG GATCATGAAC GCAATGGGTG CACACTATGT 
AAAAGTGATA GATACTGTGA ACCACATGAT TATGAATACC TCTGTCCTTG TGAATGGCAC 
CAGACAGACC ATAATAGGCA ACTAAATGAA ATAGAAAACA ATATTAAAAA GAAAGCTTGC 
AGTTGCGAAG GATTTCCATT CCATGAGGTC ATCCAAGAAT TCCTTTCGAA TAAGAATAAA 
CTGTTAAAAC CGATCAGGTA CCAAAGACCT GATTTATTAT TGTTTCAGAG ATTTACTGTT 
CAAAAAATGG AGTGGCCCAG TCACTATGCG TGTGAAAAAT TGTTGGTGCT TTTGACCCGC 
TATGACATGA TAGAAAGAAA ACTTGGTAGA AAGACTTCCA ATCAACTACA GCCAATTAGA 
ATTGTTAAAC CTCGAATCAG AAATGGAGTC TGTTGTCTTG AAATAGAATG GGAAAAGCCT 
GAACATTATT CGGTAGAAGA TACACAACCT GGAGGACTGG ATCTCCTTAC AATCGAAGAA 
GCATCACTGT TTGAAGCAGC CTATCCTGAA GTTGTAGCTA TCTACCAGAA ACAACAGTCA 
GAAACTAAAG GGAAGAAACA GAAAAATATA AAAATTAAGC CCAAAGGAAG CCATCTACCA 
GAATCAGATG ATGTGATCAG TTCCCAGTCA CTTATTACTT TAGAACCTAT ATCAAAAGCC 
TTCCCCAAGC AGAATCCCAA ATTTGATTTG GAAATTTCTC CAGATCCTAT TTTAGCACAG 
GAGTCTACTT CTCCCTCATT GAGTAGTTTT GTTTCCCCTG AAAATGCTCT TGGTTTGAAT 
TTGCAAGAAC AGTTAATGCC TTCTCCAAGA ACTTTGGCTG TAAAGCAAAC TAAAGATGTC 
AGTAATTTTC TAGTTTCAGA GTGTAGTCAG CCCAGTTCTT CATCCCATAA TATTTCTGTG 
ATTACTGATC TACACTTGAG TACCATTGAC TGGGCAGGTA CTTCTTTTAG TAATTCTCCA 
GCAGTTCAAA GAAACACTTT CTCTCAAGAT TTACAATCAG AACCTGAATC ATCAGCCATC 
CTTCCTGCCT TTGAACAGTT GTCATATGAA TCAGAACAAT GTACCTTAAG CAACAAAGTG 
TCCAATAGAG GTCTTCAGAA GAGTGGTCCT GAAGAGCATC TCCTTTCCAG CATCACTGGT 
TTACATCTTC ACGATTTGCC TCTAAAGGAA AGAATACATA TAAAATCTTC ATGCCCTCAG 
TATAATGTAG GAGCAGATGC TGACCTGGAA AGTTTACCCC TAACAATGAA TCACTCTTGC 
ATTGCTTACA GTAGCTCTGG TGGTTCATCC CATTTTTCGA AGGATCTTAC AGGGGTATAT 
CTTCAGAAAG AGCTCAGAAA CTCTAAAGTT CTAGACAGCC ACTTCCTTCA AGAAAACTTC 
AGGGTGAATA CTTCCTTACC TTATTCTTTC AATGACGAAG CAGTAAAGAC TTCCAGTCTT 
CAAGTTGGGC TGCCAACTGC TGCTGTACCT CATAATCCAA AAGTTGATGT GAAAACTAAG 
AACCTTGTCA AGAAGAGTAG TGTTTGCCTC AAGAGAGATT CCTCAGATGA AGAGAATGCT 
GCAGTGTCCT GGAAAGCTAA GTATACTGCT CCTAAATTGA AACACAGCTC TCATAATCAT 
AGCCTAGTCC ATGACAGAGA CAGTACTCAC AACAAACACA GAAATCCGAA AGATGAATCT 
AACGAAACCA AACTGTGTAC TGAGTCTTTT AAAATAGCTG AAGATGAAGA AAACAAGTTC 
TCAGATCCAG CAAGAAATCC CCAGAGTTTT CTACAATGTC ATAACAAGGA TGAGGACTCT 
GCTGACTGCT GGGAAAGTCC TCTCCCTTTA CGCCAGAGGT TAAAGCTCAG GTTCCAGAAC 
ACTCAAAGTA GACTTTATAA TACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gen1 ( XM_006239896.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239958.1 Rattus norvegicus GEN1 Holliday junction 5' flap endonuclease (Gen1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29211
Clone ID Related Accession (Same CDS sequence)	XM_006239896.2
Accession Version	XM_006239896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	flap endonuclease GEN homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGA ATGACCTGTG GCAAATTTTG GAGCCTGTAA AGCAACATAT CCACTTGCAG 
GATCTTTGTG GGAAGACCAT TGCCGTTGAT TTGAGTCTCT GGGTATGCGA GGCACAGACA 
GTGAAGAAAA TGATTGGAAC TGTCATGAAG CCCCACCTCA GGAACCTATT TTTTCGTATT 
TCATATTTAA CTCAAATGAA TGTAAAACTG GTGTTTGTGA TGGAAGGGGA GCCACCAAAG 
CTGAAAGCTG ATGTCATGAA CAAGAGGACT CAGACTCGAT ACGGGCCTTC TGGAAAATCG 
AGGTCTCAGA AAACAGGGAG ATCGCATTTT AAGTCAGTCT TACGAGAGTG CCTTGAAATG 
CTAGAGTGCC TAGGAATGCC CTGGGTCCAG GCTGCGGGGG AAGCTGAGGC CATGTGTGCT 
TACCTTAATG CCAGCGGCCA TGTGGACGGC TGCCTCACCA ACGACGGCGA TGCTTTCCTT 
TATGGAGCCC AGACTGTGTA CAGGAATTTC ACCATGAACA CAAAGGATCC ACATGTTGAC 
TGTTACACGG TATCATCTAT CAAGAGTAAG CTAGGCTTGG ATCGAGATGC TCTGGTGGGA 
CTGGCGGTAC TTCTTGGTTG TGATTACCTT CCGAAGGGAG TGCCTGGAGT TGGAAAAGAA 
CAAGCGTTAA AACTTTTGCA GATTTTGAAA GGTCAAAGTT TGCTTCAGAG GTTTAACCAG 
TGGACTGAAG AACCTGGCTA CTCTGTTCCA CAATCAGCAG CAAAAAAAGT GGTTCACTGC 
TCGGTGTGTT CCCATCCAGG TTCTCCTAAG GATCATGAAC GCAATGGGTG CACACTATGT 
AAAAGTGATA GATACTGTGA ACCACATGAT TATGAATACC TCTGTCCTTG TGAATGGCAC 
CAGACAGACC ATAATAGGCA ACTAAATGAA ATAGAAAACA ATATTAAAAA GAAAGCTTGC 
AGTTGCGAAG GATTTCCATT CCATGAGGTC ATCCAAGAAT TCCTTTCGAA TAAGAATAAA 
CTGTTAAAAC CGATCAGGTA CCAAAGACCT GATTTATTAT TGTTTCAGAG ATTTACTGTT 
CAAAAAATGG AGTGGCCCAG TCACTATGCG TGTGAAAAAT TGTTGGTGCT TTTGACCCGC 
TATGACATGA TAGAAAGAAA ACTTGGTAGA AAGACTTCCA ATCAACTACA GCCAATTAGA 
ATTGTTAAAC CTCGAATCAG AAATGGAGTC TGTTGTCTTG AAATAGAATG GGAAAAGCCT 
GAACATTATT CGGTAGAAGA TACACAACCT GGAGGACTGG ATCTCCTTAC AATCGAAGAA 
GCATCACTGT TTGAAGCAGC CTATCCTGAA GTTGTAGCTA TCTACCAGAA ACAACAGTCA 
GAAACTAAAG GGAAGAAACA GAAAAGTAAG GATATAAAAA TTAAGCCCAA AGGAAGCCAT 
CTACCAGAAT CAGATGATGT GATCAGTTCC CAGTCACTTA TTACTTTAGA ACCTATATCA 
AAAGCCTTCC CCAAGCAGAA TCCCAAATTT GATTTGGAAA TTTCTCCAGA TCCTATTTTA 
GCACAGGAGT CTACTTCTCC CTCATTGAGT AGTTTTGTTT CCCCTGAAAA TGCTCTTGGT 
TTGAATTTGC AAGAACAGTT AATGCCTTCT CCAAGAACTT TGGCTGTAAA GCAAACTAAA 
GATGTCAGTA ATTTTCTAGT TTCAGAGTGT AGTCAGCCCA GTTCTTCATC CCATAATATT 
TCTGTGATTA CTGATCTACA CTTGAGTACC ATTGACTGGG CAGGTACTTC TTTTAGTAAT 
TCTCCAGCAG TTCAAAGAAA CACTTTCTCT CAAGATTTAC AATCAGAACC TGAATCATCA 
GCCATCCTTC CTGCCTTTGA ACAGTTGTCA TATGAATCAG AACAATGTAC CTTAAGCAAC 
AAAGTGTCCA ATAGAGGTCT TCAGAAGAGT GGTCCTGAAG AGCATCTCCT TTCCAGCATC 
ACTGGTTTAC ATCTTCACGA TTTGCCTCTA AAGGAAAGAA TACATATAAA ATCTTCATGC 
CCTCAGTATA ATGTAGGAGC AGATGCTGAC CTGGAAAGTT TACCCCTAAC AATGAATCAC 
TCTTGCATTG CTTACAGTAG CTCTGGTGGT TCATCCCATT TTTCGAAGGA TCTTACAGGG 
GTATATCTTC AGAAAGAGCT CAGAAACTCT AAAGTTCTAG ACAGCCACTT CCTTCAAGAA 
AACTTCAGGG TGAATACTTC CTTACCTTAT TCTTTCAATG ACGAAGCAGT AAAGACTTCC 
AGTCTTCAAG TTGGGCTGCC AACTGCTGCT GTACCTCATA ATCCAAAAGT TGATGTGAAA 
ACTAAGAACC TTGTCAAGAA GAGTAGTGTT TGCCTCAAGA GAGATTCCTC AGATGAAGAG 
AATGCTGCAG TGTCCTGGAA AGCTAAGTAT ACTGCTCCTA AATTGAAACA CAGCTCTCAT 
AATCATAGCC TAGTCCATGA CAGAGACAGT ACTCACAACA AACACAGAAA TCCGAAAGAT 
GAATCTAACG AAACCAAACT GTGTACTGAG TCTTTTAAAA TAGCTGAAGA TGAAGAAAAC 
AAGTTCTCAG ATCCAGCAAG AAATCCCCAG AGTTTTCTAC AATGTCATAA CAAGGATGAG 
GACTCTGCTG ACTGCTGGGA AAGTCCTCTC CCTTTACGCC AGAGGTTAAA GCTCAGGTTC 
CAGAACACTC AAAGTAGACT TTATAATACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239958.1
CDS	157..2889
Translation	MGVNDLWQILEPVKQHIHLQDLCGKTIAVDLSLWVCEAQTVKKMIGTVMKPHLRNLFFRISYLTQMNVKLVFVMEGEPPKLKADVMNKRTQTRYGPSGKSRSQKTGRSHFKSVLRECLEMLECLGMPWVQAAGEAEAMCAYLNASGHVDGCLTNDGDAFLYGAQTVYRNFTMNTKDPHVDCYTVSSIKSKLGLDRDALVGLAVLLGCDYLPKGVPGVGKEQALKLLQILKGQSLLQRFNQWTEEPGYSVPQSAAKKVVHCSVCSHPGSPKDHERNGCTLCKSDRYCEPHDYEYLCPCEWHQTDHNRQLNEIENNIKKKACSCEGFPFHEVIQEFLSNKNKLLKPIRYQRPDLLLFQRFTVQKMEWPSHYACEKLLVLLTRYDMIERKLGRKTSNQLQPIRIVKPRIRNGVCCLEIEWEKPEHYSVEDTQPGGLDLLTIEEASLFEAAYPEVVAIYQKQQSETKGKKQKSKDIKIKPKGSHLPESDDVISSQSLITLEPISKAFPKQNPKFDLEISPDPILAQESTSPSLSSFVSPENALGLNLQEQLMPSPRTLAVKQTKDVSNFLVSECSQPSSSSHNISVITDLHLSTIDWAGTSFSNSPAVQRNTFSQDLQSEPESSAILPAFEQLSYESEQCTLSNKVSNRGLQKSGPEEHLLSSITGLHLHDLPLKERIHIKSSCPQYNVGADADLESLPLTMNHSCIAYSSSGGSSHFSKDLTGVYLQKELRNSKVLDSHFLQENFRVNTSLPYSFNDEAVKTSSLQVGLPTAAVPHNPKVDVKTKNLVKKSSVCLKRDSSDEENAAVSWKAKYTAPKLKHSSHNHSLVHDRDSTHNKHRNPKDESNETKLCTESFKIAEDEENKFSDPARNPQSFLQCHNKDEDSADCWESPLPLRQRLKLRFQNTQSRLYNT

Target ORF information:

RefSeq Version	XM_006239896.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GEN1 Holliday junction 5' flap endonuclease (Gen1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239896.2

ORF Insert Sequence:

ATGGGAGTGA ATGACCTGTG GCAAATTTTG GAGCCTGTAA AGCAACATAT CCACTTGCAG 
GATCTTTGTG GGAAGACCAT TGCCGTTGAT TTGAGTCTCT GGGTATGCGA GGCACAGACA 
GTGAAGAAAA TGATTGGAAC TGTCATGAAG CCCCACCTCA GGAACCTATT TTTTCGTATT 
TCATATTTAA CTCAAATGAA TGTAAAACTG GTGTTTGTGA TGGAAGGGGA GCCACCAAAG 
CTGAAAGCTG ATGTCATGAA CAAGAGGACT CAGACTCGAT ACGGGCCTTC TGGAAAATCG 
AGGTCTCAGA AAACAGGGAG ATCGCATTTT AAGTCAGTCT TACGAGAGTG CCTTGAAATG 
CTAGAGTGCC TAGGAATGCC CTGGGTCCAG GCTGCGGGGG AAGCTGAGGC CATGTGTGCT 
TACCTTAATG CCAGCGGCCA TGTGGACGGC TGCCTCACCA ACGACGGCGA TGCTTTCCTT 
TATGGAGCCC AGACTGTGTA CAGGAATTTC ACCATGAACA CAAAGGATCC ACATGTTGAC 
TGTTACACGG TATCATCTAT CAAGAGTAAG CTAGGCTTGG ATCGAGATGC TCTGGTGGGA 
CTGGCGGTAC TTCTTGGTTG TGATTACCTT CCGAAGGGAG TGCCTGGAGT TGGAAAAGAA 
CAAGCGTTAA AACTTTTGCA GATTTTGAAA GGTCAAAGTT TGCTTCAGAG GTTTAACCAG 
TGGACTGAAG AACCTGGCTA CTCTGTTCCA CAATCAGCAG CAAAAAAAGT GGTTCACTGC 
TCGGTGTGTT CCCATCCAGG TTCTCCTAAG GATCATGAAC GCAATGGGTG CACACTATGT 
AAAAGTGATA GATACTGTGA ACCACATGAT TATGAATACC TCTGTCCTTG TGAATGGCAC 
CAGACAGACC ATAATAGGCA ACTAAATGAA ATAGAAAACA ATATTAAAAA GAAAGCTTGC 
AGTTGCGAAG GATTTCCATT CCATGAGGTC ATCCAAGAAT TCCTTTCGAA TAAGAATAAA 
CTGTTAAAAC CGATCAGGTA CCAAAGACCT GATTTATTAT TGTTTCAGAG ATTTACTGTT 
CAAAAAATGG AGTGGCCCAG TCACTATGCG TGTGAAAAAT TGTTGGTGCT TTTGACCCGC 
TATGACATGA TAGAAAGAAA ACTTGGTAGA AAGACTTCCA ATCAACTACA GCCAATTAGA 
ATTGTTAAAC CTCGAATCAG AAATGGAGTC TGTTGTCTTG AAATAGAATG GGAAAAGCCT 
GAACATTATT CGGTAGAAGA TACACAACCT GGAGGACTGG ATCTCCTTAC AATCGAAGAA 
GCATCACTGT TTGAAGCAGC CTATCCTGAA GTTGTAGCTA TCTACCAGAA ACAACAGTCA 
GAAACTAAAG GGAAGAAACA GAAAAGTAAG GATATAAAAA TTAAGCCCAA AGGAAGCCAT 
CTACCAGAAT CAGATGATGT GATCAGTTCC CAGTCACTTA TTACTTTAGA ACCTATATCA 
AAAGCCTTCC CCAAGCAGAA TCCCAAATTT GATTTGGAAA TTTCTCCAGA TCCTATTTTA 
GCACAGGAGT CTACTTCTCC CTCATTGAGT AGTTTTGTTT CCCCTGAAAA TGCTCTTGGT 
TTGAATTTGC AAGAACAGTT AATGCCTTCT CCAAGAACTT TGGCTGTAAA GCAAACTAAA 
GATGTCAGTA ATTTTCTAGT TTCAGAGTGT AGTCAGCCCA GTTCTTCATC CCATAATATT 
TCTGTGATTA CTGATCTACA CTTGAGTACC ATTGACTGGG CAGGTACTTC TTTTAGTAAT 
TCTCCAGCAG TTCAAAGAAA CACTTTCTCT CAAGATTTAC AATCAGAACC TGAATCATCA 
GCCATCCTTC CTGCCTTTGA ACAGTTGTCA TATGAATCAG AACAATGTAC CTTAAGCAAC 
AAAGTGTCCA ATAGAGGTCT TCAGAAGAGT GGTCCTGAAG AGCATCTCCT TTCCAGCATC 
ACTGGTTTAC ATCTTCACGA TTTGCCTCTA AAGGAAAGAA TACATATAAA ATCTTCATGC 
CCTCAGTATA ATGTAGGAGC AGATGCTGAC CTGGAAAGTT TACCCCTAAC AATGAATCAC 
TCTTGCATTG CTTACAGTAG CTCTGGTGGT TCATCCCATT TTTCGAAGGA TCTTACAGGG 
GTATATCTTC AGAAAGAGCT CAGAAACTCT AAAGTTCTAG ACAGCCACTT CCTTCAAGAA 
AACTTCAGGG TGAATACTTC CTTACCTTAT TCTTTCAATG ACGAAGCAGT AAAGACTTCC 
AGTCTTCAAG TTGGGCTGCC AACTGCTGCT GTACCTCATA ATCCAAAAGT TGATGTGAAA 
ACTAAGAACC TTGTCAAGAA GAGTAGTGTT TGCCTCAAGA GAGATTCCTC AGATGAAGAG 
AATGCTGCAG TGTCCTGGAA AGCTAAGTAT ACTGCTCCTA AATTGAAACA CAGCTCTCAT 
AATCATAGCC TAGTCCATGA CAGAGACAGT ACTCACAACA AACACAGAAA TCCGAAAGAT 
GAATCTAACG AAACCAAACT GTGTACTGAG TCTTTTAAAA TAGCTGAAGA TGAAGAAAAC 
AAGTTCTCAG ATCCAGCAAG AAATCCCCAG AGTTTTCTAC AATGTCATAA CAAGGATGAG 
GACTCTGCTG ACTGCTGGGA AAGTCCTCTC CCTTTACGCC AGAGGTTAAA GCTCAGGTTC 
CAGAACACTC AAAGTAGACT TTATAATACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Resolution of single and double Holliday junction recombination intermediates by GEN1.
Proceedings of the National Academy of Sciences of the United States of America114(3)443-450(2017 Jan)
Shah Punatar R,Martin MJ,Wyatt HD,Chan YW,West SC

GEN1 promotes Holliday junction resolution by a coordinated nick and counter-nick mechanism.
Nucleic acids research43(22)10882-92(2015 Dec)
Chan YW,West S

Human Holliday junction resolvase GEN1 uses a chromodomain for efficient DNA recognition and cleavage.
eLife4(2015 Dec)
Lee SH,Princz LN,Kl?gel MF,Habermann B,Pfander B,Biert?mpfel C

Human GEN1 and the SLX4-associated nucleases MUS81 and SLX1 are essential for the resolution of replication-induced Holliday junctions.
Cell reports5(1)207-15(2013 Oct)
Garner E,Kim Y,Lach FP,Kottemann MC,Smogorzewska A

A novel role of human holliday junction resolvase GEN1 in the maintenance of centrosome integrity.
PloS one7(11)e49687(2012)
Gao M,Rendtlew Danielsen J,Wei LZ,Zhou DP,Xu Q,Li MM,Wang ZQ,Tong WM,Yang YG