Laptm4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Laptm4a
Entrez Gene ID	298875
Full Name	lysosomal protein transmembrane 4 alpha
General protein information	Preferred Names lysosomal protein transmembrane 4 alpha Names lysosomal-associated transmembrane protein 4A lysosomal-associated protein transmembrane 4 alpha lysosomal-associated protein transmembrane 4A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	mouse homolog regulates intracellular distribution of amphipathic solutes; may regulate drug sensitivity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199384.2	NP_955416.1	lysosomal-associated transmembrane protein 4A

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LAPTM4A	NP_001026263.1
Drosophila melanogaster (fruit fly)	CG14767	NP_652400.3
Pan troglodytes (chimpanzee)	LAPTM4A	XP_003949812.1
	LAPTM4A	XP_001095082.1
Mus musculus (house mouse)	Laptm4a	NP_032666.2
Bos taurus (cattle)	LAPTM4A	NP_991368.1
Homo sapiens (human)	LAPTM4A	NP_055528.1
Canis lupus familiaris (dog)	LAPTM4A	XP_003639626.1
Rattus norvegicus (Norway rat)	Laptm4a	NP_955416.1
Danio rerio (zebrafish)	laptm4a	NP_999850.2
Xenopus tropicalis (tropical clawed frog)	laptm4a	NP_001004797.1

Gene Ontology
Bibliography

Related articles in PubMed

A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.
Milkereit R, Rotin D
PloS one6(11)e27478(2011)

Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.
Mortensen M, Ferguson DJ, Edelmann M, Kessler B, Morten KJ, Komatsu M, Simon AK
Proceedings of the National Academy of Sciences of the United States of America107(2)832-7(2010 Jan)

Lysosome-associated protein transmembrane 4 alpha (LAPTM4 alpha) requires two tandemly arranged tyrosine-based signals for sorting to lysosomes.
Hogue DL, Nash C, Ling V, Hobman TC
The Biochemical journal365(Pt 3)721-30(2002 Aug)

A visual intracellular classification strategy for uncharacterized human proteins.
Hoja MR, Wahlestedt C, H??g C
Experimental cell research259(1)239-46(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Laptm4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Laptm4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05275	NM_199384.2 Latest version!	Rattus norvegicus lysosomal protein transmembrane 4 alpha (Laptm4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Laptm4a ( NM_199384.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_955416.1 Rattus norvegicus lysosomal protein transmembrane 4 alpha (Laptm4a), mRNA.

Price & Availability↑

CloneID	ORa05275
Clone ID Related Accession (Same CDS sequence)	NM_199384.2
Accession Version	NM_199384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal-associated transmembrane protein 4A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC063179.1. On Aug 30, 2012 this sequence version replaced NM_199384.1. ##Evidence-Data-START## Transcript exon combination :: BC063179.1, FQ212732.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGTCCA TGACTTTCAA ACGGAGCCGC AGCGACCGGT TCTACAGCAC CCGGTGCTGC 
GGCTGTTGCC ATGTCCGCAC CGGGACGATC ATCCTGGGGA CCTGGTACAT GGTGGTCAAC 
CTATTGATGG CAATTTTGCT GACTGTGGAG GTGACTCATC CAAATTCCAT GCCAGCTGTC 
AACATTCAGT ATGAAGTCAT TGGTAATTAC TATTCATCTG AGAGAATGGC TGATAATGCC 
TGCGTTCTTT TTGCCGTCTC TGTTCTTATG TTTATAATCA GTTCAATGCT GGTTTATGGA 
GCCATTTCTT ATCAAGTGGG CTGGCTGATT CCTTTCTTCT GCTACCGACT TTTTGACTTC 
GTTCTCAGTT GTCTGGTAGC CATCAGTTCT CTCACTTACT TGCCAAGAAT CAAAGAATAT 
CTGGATCAGT TGCCTGATTT CCCCTACAAA GATGACCTCC TGGCATTGGA CTCCAGCTGC 
CTTCTGTTCA TTGTACTTGT GTTCTTTGTG GTATTCATCA TTTTTAAGGC TTACCTAATC 
AATTGTGTTT GGAACTGCTA CAAATACATC AACAATAGAA ATGTGCCAGA GATTGCCGTG 
TATCCTGCCT TTGAAGCACC TCCACAGTAC GTTTTGCCAA CCTATGAAAT GGCTGTGAAG 
ATTCCTGAGA AAGAGCCACC ACCTCCTTAC CTGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955416.1
CDS	79..780
Translation	MVSMTFKRSRSDRFYSTRCCGCCHVRTGTIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNYYSSERMADNACVLFAVSVLMFIISSMLVYGAISYQVGWLIPFFCYRLFDFVLSCLVAISSLTYLPRIKEYLDQLPDFPYKDDLLALDSSCLLFIVLVFFVVFIIFKAYLINCVWNCYKYINNRNVPEIAVYPAFEAPPQYVLPTYEMAVKIPEKEPPPPYLPA

Target ORF information:

RefSeq Version	NM_199384.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lysosomal protein transmembrane 4 alpha (Laptm4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199384.2

ORF Insert Sequence:

ATGGTGTCCA TGACTTTCAA ACGGAGCCGC AGCGACCGGT TCTACAGCAC CCGGTGCTGC 
GGCTGTTGCC ATGTCCGCAC CGGGACGATC ATCCTGGGGA CCTGGTACAT GGTGGTCAAC 
CTATTGATGG CAATTTTGCT GACTGTGGAG GTGACTCATC CAAATTCCAT GCCAGCTGTC 
AACATTCAGT ATGAAGTCAT TGGTAATTAC TATTCATCTG AGAGAATGGC TGATAATGCC 
TGCGTTCTTT TTGCCGTCTC TGTTCTTATG TTTATAATCA GTTCAATGCT GGTTTATGGA 
GCCATTTCTT ATCAAGTGGG CTGGCTGATT CCTTTCTTCT GCTACCGACT TTTTGACTTC 
GTTCTCAGTT GTCTGGTAGC CATCAGTTCT CTCACTTACT TGCCAAGAAT CAAAGAATAT 
CTGGATCAGT TGCCTGATTT CCCCTACAAA GATGACCTCC TGGCATTGGA CTCCAGCTGC 
CTTCTGTTCA TTGTACTTGT GTTCTTTGTG GTATTCATCA TTTTTAAGGC TTACCTAATC 
AATTGTGTTT GGAACTGCTA CAAATACATC AACAATAGAA ATGTGCCAGA GATTGCCGTG 
TATCCTGCCT TTGAAGCACC TCCACAGTAC GTTTTGCCAA CCTATGAAAT GGCTGTGAAG 
ATTCCTGAGA AAGAGCCACC ACCTCCTTAC CTGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.
PloS one6(11)e27478(2011)
Milkereit R,Rotin D

Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.
Proceedings of the National Academy of Sciences of the United States of America107(2)832-7(2010 Jan)
Mortensen M,Ferguson DJ,Edelmann M,Kessler B,Morten KJ,Komatsu M,Simon AK

Lysosome-associated protein transmembrane 4 alpha (LAPTM4 alpha) requires two tandemly arranged tyrosine-based signals for sorting to lysosomes.
The Biochemical journal365(Pt 3)721-30(2002 Aug)
Hogue DL,Nash C,Ling V,Hobman TC

A visual intracellular classification strategy for uncharacterized human proteins.
Experimental cell research259(1)239-46(2000 Aug)
Hoja MR,Wahlestedt C,H??g C