Kcnmb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnmb1
Entrez Gene ID	29747
Full Name	potassium calcium-activated channel subfamily M regulatory beta subunit 1
Synonyms	BKbeta,BKbeta1,k(VCA)beta-1,slo-beta,slo-beta-1
General protein information	Preferred Names potassium calcium-activated channel subfamily M regulatory beta subunit 1 Names calcium-activated potassium channel subunit beta-1 BK channel subunit beta-1 calcium-activated potassium channel beta subunit calcium-activated potassium channel subunit beta calcium-activated potassium channel, subfamily M subunit beta-1 charybdotoxin receptor subunit beta-1 maxi K channel subunit beta-1 potassium channel subfamily M regulatory beta subunit 1 potassium large conductance calcium-activated channel, subfamily M, beta member 1 slowpoke-beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	modulatory subunit of the MaxiK large conductance potassium channel; voltage and calcium-sensitive potassium channel [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019273.1	NP_062146.1	calcium-activated potassium channel subunit beta-1
XM_006246089.3	XP_006246151.1	calcium-activated potassium channel subunit beta-1 isoform X1
XM_008767564.2	XP_008765786.1	calcium-activated potassium channel subunit beta-1 isoform X2
XM_008767563.2	XP_008765785.1	calcium-activated potassium channel subunit beta-1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KCNMB1	NP_001001141.1
Mus musculus (house mouse)	Kcnmb1	NP_112446.2
Pan troglodytes (chimpanzee)	KCNMB1	XP_518094.1
Gallus gallus (chicken)	KCNMB1	NP_989933.1
Canis lupus familiaris (dog)	KCNMB1	NP_001003299.1
Homo sapiens (human)	KCNMB1	NP_004128.1
	KCNMB1	XP_001092823.1
Rattus norvegicus (Norway rat)	Kcnmb1	NP_062146.1

Gene Ontology
Bibliography

Related articles in PubMed

BK
Zhang Y, Liao J, Zhang L, Li S, Wu Y, Shi L
Hypertension research : official journal of the Japanese Society of Hypertension(2017 Nov)

Membrane depolarization activates BK channels through ROCK-mediated ?1 subunit surface trafficking to limit vasoconstriction.
Leo MD, Zhai X, Muralidharan P, Kuruvilla KP, Bulley S, Boop FA, Jaggar JH
Science signaling10(478)(2017 May)

Regulation of Coronary Arterial Large Conductance Ca2+-Activated K+ Channel Protein Expression and Function by n-3 Polyunsaturated Fatty Acids in Diabetic Rats.
Tang X, Qian LL, Wang RX, Yao Y, Dang SP, Wu Y, Wang W, Ji Y, Sun MQ, Xia DY, Liu XY, Zhang DM, Chai Q, Lu T
Journal of vascular research54(6)329-343(2017)

Activation of calcium- and voltage-gated potassium channels of large conductance by leukotriene B4.
Bukiya AN, McMillan J, Liu J, Shivakumar B, Parrill AL, Dopico AM
The Journal of biological chemistry289(51)35314-25(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

n-3 PUFAs protect the coronary BK channel function and coronary vasoreactivity in diabetic rats as a result of not only increasing BK-beta1 protein expressions, but also decreasing coronary artery tension and coronary smooth muscle cytosolic Ca2+ concentrations.
Title: Regulation of Coronary Arterial Large Conductance Ca2+-Activated K+ Channel Protein Expression and Function by n-3 Polyunsaturated Fatty Acids in Diabetic Rats.

Data show that leukotriene B4, but not other leukotrienes of the LT4 family, activates recombinant (cbv1-beta1) Lrge-conductance calcium-activated potassium (BK) channels.
Title: Activation of calcium- and voltage-gated potassium channels of large conductance by leukotriene B4.

aging decreases capability of MaxiK channel in regulating vascular tone in the MA, which may be partially mediated by unparallel downregulation of alpha- and beta1-subunit expression
Title: Aging decreases the contribution of MaxiK channel in regulating vascular tone in mesenteric artery by unparallel downregulation of ?- and ?1-subunit expression.

The expression of BK(Ca) beta1- but not alpha-subunit was significantly enhanced after exercise training
Title: Aerobic exercise increases BK(Ca) channel contribution to regulation of mesenteric arterial tone by upregulating ?1-subunit.

BKbeta1 transcripts and protein are detected in several astrocytic populations and cultured cells, to a lesser degree than BKbeta4 channels.
Title: Expression of the voltage- and Ca2+-dependent BK potassium channel subunits BK?1 and BK?4 in rodent astrocytes.

The following Kcnmb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnmb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04228	NM_019273.1 Latest version!	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa32938	XM_008767563.2 Latest version!	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa32938	XM_006246089.3 Latest version!	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa32939	XM_008767564.2 Latest version!	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnmb1 ( NM_019273.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062146.1 Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), mRNA.

Price & Availability↑

CloneID	ORa04228
Clone ID Related Accession (Same CDS sequence)	NM_019273.1
Accession Version	NM_019273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	calcium-activated potassium channel subunit beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF020712.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF020712.1, FM030431.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689601, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAAGA AGCTGGTGAT GGCCCAGAAG CGCGGAGAGA CTCGAGCCCT CTGCCTGGGG 
GTGGCGATGG TCGTGTGTGC CGCCATCACT TACTACATCC TGGGTACCAC TGTGCTGCCC 
CTCTACCAGA AAAGTGTGTG GACCCAGGAA TCCACCTGTC ACTTGGTTGA AACCAACATC 
AAGGACCAGG AAGAGCTGGA GGGCAGGAAG GTGCCCCAGT ACCCATGCCT TTGGGTCAAT 
GTATCAGCTG TGGGCAGATG GGCCATGCTG TATCACACAG AAGACACTCG GGATCAAAAC 
CAACAGTGCT CCTATATCCC CAGGAACCTG GACAACTACC AGACAGCCTT GGTTGATGTG 
AAGAAGGTCA GAGCCAATTT CTACAAACAC CATAATTTCT ATTGCTTTTC TGCACCTCAA 
GTCAATGAGA CCAGTGTTGT GTACCAGCGC CTCTATGGGC CCCAAATCCT CCTCTTCTCC 
TTCTTCTGGC CCACCTTCCT GCTGACTGGT GGCCTTCTTA TCATTGCCAT GGTAAAGCTC 
AACAGGTCCC TCTCTGTCTT GGCAGCTCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062146.1
CDS	314..889
Translation	MGKKLVMAQKRGETRALCLGVAMVVCAAITYYILGTTVLPLYQKSVWTQESTCHLVETNIKDQEELEGRKVPQYPCLWVNVSAVGRWAMLYHTEDTRDQNQQCSYIPRNLDNYQTALVDVKKVRANFYKHHNFYCFSAPQVNETSVVYQRLYGPQILLFSFFWPTFLLTGGLLIIAMVKLNRSLSVLAAQK

Target ORF information:

RefSeq Version	NM_019273.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019273.1

ORF Insert Sequence:

ATGGGGAAGA AGCTGGTGAT GGCCCAGAAG CGCGGAGAGA CTCGAGCCCT CTGCCTGGGG 
GTGGCGATGG TCGTGTGTGC CGCCATCACT TACTACATCC TGGGTACCAC TGTGCTGCCC 
CTCTACCAGA AAAGTGTGTG GACCCAGGAA TCCACCTGTC ACTTGGTTGA AACCAACATC 
AAGGACCAGG AAGAGCTGGA GGGCAGGAAG GTGCCCCAGT ACCCATGCCT TTGGGTCAAT 
GTATCAGCTG TGGGCAGATG GGCCATGCTG TATCACACAG AAGACACTCG GGATCAAAAC 
CAACAGTGCT CCTATATCCC CAGGAACCTG GACAACTACC AGACAGCCTT GGTTGATGTG 
AAGAAGGTCA GAGCCAATTT CTACAAACAC CATAATTTCT ATTGCTTTTC TGCACCTCAA 
GTCAATGAGA CCAGTGTTGT GTACCAGCGC CTCTATGGGC CCCAAATCCT CCTCTTCTCC 
TTCTTCTGGC CCACCTTCCT GCTGACTGGT GGCCTTCTTA TCATTGCCAT GGTAAAGCTC 
AACAGGTCCC TCTCTGTCTT GGCAGCTCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnmb1 ( XM_006246089.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246151.1 Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa32938
Clone ID Related Accession (Same CDS sequence)	XM_006246089.3 , XM_008767563.2
Accession Version	XM_006246089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calcium-activated potassium channel subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCTGGTGAT GGCCCAGAAG CGCGGAGAGA CGCGAGCCCT CTGCCTGGGG 
GTGGCGATGG TCGTGTGTGC CGCCATCACT TACTACATCC TGGGTACCAC TGTGCTGCCC 
CTCTACCAGA AAAGTGTGTG GACCCAGGAA TCCACCTGTC ACTTGGTTGA AACCAACATC 
AAGGACCAGG AAGAGCTGGA GGGCAGGAAG GTGCCCCAGT ACCCATGCCT TTGGGTCAAT 
GTATCAGCTG TGGGCAGATG GGCCATGCTG TATCACACAG AAGACACTCG GGATCAAAAC 
CAACAGTGCT CCTATATCCC CAGGAACCTG GACAACTACC AGACAGCCTT GGTTGATGTG 
AAGAAGGTCA GAGCCAATTT CTACAAACAC CATAATTTCT ATTGCTTTTC TGCACCTCAA 
GTCAATGAGA CCAGTGTTGT GTACCAGCGC CTCTATGGGC CCCAAATCCT CCTCTTCTCC 
TTCTTCTGGC CCACCTTCCT GCTGACTGGT GGCCTTCTTA TCATTGCCAT GGTAAAGCTC 
AACAGGTCCC TCTCTGTCTT GGCAGCTCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246151.1
CDS	2045..2620
Translation	MGKKLVMAQKRGETRALCLGVAMVVCAAITYYILGTTVLPLYQKSVWTQESTCHLVETNIKDQEELEGRKVPQYPCLWVNVSAVGRWAMLYHTEDTRDQNQQCSYIPRNLDNYQTALVDVKKVRANFYKHHNFYCFSAPQVNETSVVYQRLYGPQILLFSFFWPTFLLTGGLLIIAMVKLNRSLSVLAAQK

Target ORF information:

RefSeq Version	XM_006246089.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246089.3

ORF Insert Sequence:

ATGGGGAAGA AGCTGGTGAT GGCCCAGAAG CGCGGAGAGA CGCGAGCCCT CTGCCTGGGG 
GTGGCGATGG TCGTGTGTGC CGCCATCACT TACTACATCC TGGGTACCAC TGTGCTGCCC 
CTCTACCAGA AAAGTGTGTG GACCCAGGAA TCCACCTGTC ACTTGGTTGA AACCAACATC 
AAGGACCAGG AAGAGCTGGA GGGCAGGAAG GTGCCCCAGT ACCCATGCCT TTGGGTCAAT 
GTATCAGCTG TGGGCAGATG GGCCATGCTG TATCACACAG AAGACACTCG GGATCAAAAC 
CAACAGTGCT CCTATATCCC CAGGAACCTG GACAACTACC AGACAGCCTT GGTTGATGTG 
AAGAAGGTCA GAGCCAATTT CTACAAACAC CATAATTTCT ATTGCTTTTC TGCACCTCAA 
GTCAATGAGA CCAGTGTTGT GTACCAGCGC CTCTATGGGC CCCAAATCCT CCTCTTCTCC 
TTCTTCTGGC CCACCTTCCT GCTGACTGGT GGCCTTCTTA TCATTGCCAT GGTAAAGCTC 
AACAGGTCCC TCTCTGTCTT GGCAGCTCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnmb1 ( XM_008767563.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765785.1 Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32938
Clone ID Related Accession (Same CDS sequence)	XM_006246089.3 , XM_008767563.2
Accession Version	XM_008767563.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calcium-activated potassium channel subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCTGGTGAT GGCCCAGAAG CGCGGAGAGA CGCGAGCCCT CTGCCTGGGG 
GTGGCGATGG TCGTGTGTGC CGCCATCACT TACTACATCC TGGGTACCAC TGTGCTGCCC 
CTCTACCAGA AAAGTGTGTG GACCCAGGAA TCCACCTGTC ACTTGGTTGA AACCAACATC 
AAGGACCAGG AAGAGCTGGA GGGCAGGAAG GTGCCCCAGT ACCCATGCCT TTGGGTCAAT 
GTATCAGCTG TGGGCAGATG GGCCATGCTG TATCACACAG AAGACACTCG GGATCAAAAC 
CAACAGTGCT CCTATATCCC CAGGAACCTG GACAACTACC AGACAGCCTT GGTTGATGTG 
AAGAAGGTCA GAGCCAATTT CTACAAACAC CATAATTTCT ATTGCTTTTC TGCACCTCAA 
GTCAATGAGA CCAGTGTTGT GTACCAGCGC CTCTATGGGC CCCAAATCCT CCTCTTCTCC 
TTCTTCTGGC CCACCTTCCT GCTGACTGGT GGCCTTCTTA TCATTGCCAT GGTAAAGCTC 
AACAGGTCCC TCTCTGTCTT GGCAGCTCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765785.1
CDS	2863..3438
Translation	MGKKLVMAQKRGETRALCLGVAMVVCAAITYYILGTTVLPLYQKSVWTQESTCHLVETNIKDQEELEGRKVPQYPCLWVNVSAVGRWAMLYHTEDTRDQNQQCSYIPRNLDNYQTALVDVKKVRANFYKHHNFYCFSAPQVNETSVVYQRLYGPQILLFSFFWPTFLLTGGLLIIAMVKLNRSLSVLAAQK

Target ORF information:

RefSeq Version	XM_008767563.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767563.2

ORF Insert Sequence:

ATGGGGAAGA AGCTGGTGAT GGCCCAGAAG CGCGGAGAGA CGCGAGCCCT CTGCCTGGGG 
GTGGCGATGG TCGTGTGTGC CGCCATCACT TACTACATCC TGGGTACCAC TGTGCTGCCC 
CTCTACCAGA AAAGTGTGTG GACCCAGGAA TCCACCTGTC ACTTGGTTGA AACCAACATC 
AAGGACCAGG AAGAGCTGGA GGGCAGGAAG GTGCCCCAGT ACCCATGCCT TTGGGTCAAT 
GTATCAGCTG TGGGCAGATG GGCCATGCTG TATCACACAG AAGACACTCG GGATCAAAAC 
CAACAGTGCT CCTATATCCC CAGGAACCTG GACAACTACC AGACAGCCTT GGTTGATGTG 
AAGAAGGTCA GAGCCAATTT CTACAAACAC CATAATTTCT ATTGCTTTTC TGCACCTCAA 
GTCAATGAGA CCAGTGTTGT GTACCAGCGC CTCTATGGGC CCCAAATCCT CCTCTTCTCC 
TTCTTCTGGC CCACCTTCCT GCTGACTGGT GGCCTTCTTA TCATTGCCAT GGTAAAGCTC 
AACAGGTCCC TCTCTGTCTT GGCAGCTCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnmb1 ( XM_008767564.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765786.1 Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa32939
Clone ID Related Accession (Same CDS sequence)	XM_008767564.2
Accession Version	XM_008767564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calcium-activated potassium channel subunit beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCTGGTGAT GGCCCAGAAG CGCGGAGAGA CGCGAGCCCT CTGCCTGGGG 
GTGGCGATGG TCGTGTGTGC CGCCATCACT TACTACATCC TGGGTACCAC TGTGCTGCCC 
CTCTACCAGA AAAGTGTGTG GACCCAGGAA TCCACCTGTC ACTTGGTTGA AACCAACATC 
AAGGACCAGG AAGAGCTGGA GGGCAGGAAG GTGCCCCAGT ACCCATGCCT TTGGGTCAAT 
GTATCAGCTG TGGGCAGATG GGCCATGCTG TATCACACAG AAGACACTCG GGATCAAAAC 
CAACAGTGCT CCTATATCCC CAGGAACCTG GACAACTACC AGACAGCCTT GGTTGATGTG 
AAGAAGCTCA GAAGTAGACC AATCTCTTCT GCACAGCAGC ATGGAGTCAC CAGGAACGGC 
AGGGGTCCCG GGCAGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765786.1
CDS	2997..3437
Translation	MGKKLVMAQKRGETRALCLGVAMVVCAAITYYILGTTVLPLYQKSVWTQESTCHLVETNIKDQEELEGRKVPQYPCLWVNVSAVGRWAMLYHTEDTRDQNQQCSYIPRNLDNYQTALVDVKKLRSRPISSAQQHGVTRNGRGPGQA

Target ORF information:

RefSeq Version	XM_008767564.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 1 (Kcnmb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767564.2

ORF Insert Sequence:

ATGGGGAAGA AGCTGGTGAT GGCCCAGAAG CGCGGAGAGA CGCGAGCCCT CTGCCTGGGG 
GTGGCGATGG TCGTGTGTGC CGCCATCACT TACTACATCC TGGGTACCAC TGTGCTGCCC 
CTCTACCAGA AAAGTGTGTG GACCCAGGAA TCCACCTGTC ACTTGGTTGA AACCAACATC 
AAGGACCAGG AAGAGCTGGA GGGCAGGAAG GTGCCCCAGT ACCCATGCCT TTGGGTCAAT 
GTATCAGCTG TGGGCAGATG GGCCATGCTG TATCACACAG AAGACACTCG GGATCAAAAC 
CAACAGTGCT CCTATATCCC CAGGAACCTG GACAACTACC AGACAGCCTT GGTTGATGTG 
AAGAAGCTCA GAAGTAGACC AATCTCTTCT GCACAGCAGC ATGGAGTCAC CAGGAACGGC 
AGGGGTCCCG GGCAGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BK
Hypertension research : official journal of the Japanese Society of Hypertension(2017 Nov)
Zhang Y,Liao J,Zhang L,Li S,Wu Y,Shi L

Membrane depolarization activates BK channels through ROCK-mediated ?1 subunit surface trafficking to limit vasoconstriction.
Science signaling10(478)(2017 May)
Leo MD,Zhai X,Muralidharan P,Kuruvilla KP,Bulley S,Boop FA,Jaggar JH

Regulation of Coronary Arterial Large Conductance Ca2+-Activated K+ Channel Protein Expression and Function by n-3 Polyunsaturated Fatty Acids in Diabetic Rats.
Journal of vascular research54(6)329-343(2017)
Tang X,Qian LL,Wang RX,Yao Y,Dang SP,Wu Y,Wang W,Ji Y,Sun MQ,Xia DY,Liu XY,Zhang DM,Chai Q,Lu T

Activation of calcium- and voltage-gated potassium channels of large conductance by leukotriene B4.
The Journal of biological chemistry289(51)35314-25(2014 Dec)
Bukiya AN,McMillan J,Liu J,Shivakumar B,Parrill AL,Dopico AM

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Kcnmb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

オンラインフォーム