F2rl2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	F2rl2
Entrez Gene ID	29636
Full Name	coagulation factor II (thrombin) receptor-like 2
Synonyms	PAR3
General protein information	Preferred Names coagulation factor II (thrombin) receptor-like 2 Names proteinase-activated receptor 3 PAR-3 coagulation factor II receptor-like 2 protease-activated receptor 3 thrombin receptor-like 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	may play a role in response to injury and ischemia [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053313.1	NP_445765.1	proteinase-activated receptor 3 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	F2RL2	XP_849866.1
Danio rerio (zebrafish)	LOC100333265	XP_002665915.2
	F2RL2	XP_001106069.1
Mus musculus (house mouse)	F2rl2	NP_034300.3
Rattus norvegicus (Norway rat)	F2rl2	NP_445765.1
Homo sapiens (human)	F2RL2	NP_004092.1
Bos taurus (cattle)	F2RL2	NP_001033622.2
Pan troglodytes (chimpanzee)	F2RL2	XP_003310722.1
Gallus gallus (chicken)	F2RL2	XP_001231246.1
Xenopus tropicalis (tropical clawed frog)	f2rl2	NP_001039234.1
Danio rerio (zebrafish)	f2rl2	XP_005165306.1

Gene Ontology
Bibliography

Related articles in PubMed

Cross Talk Pathways Between Coagulation and Inflammation.
Foley JH, Conway EM
Circulation research118(9)1392-408(2016 Apr)

Differential expression, time course and distribution of four PARs in rats with endotoxin-induced acute lung injury.
Jesmin S, Gando S, Zaedi S, Sakuraya F
Inflammation30(1-2)14-27(2007 Apr)

The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface.
Cappello S, Attardo A, Wu X, Iwasato T, Itohara S, Wilsch-Br?uninger M, Eilken HM, Rieger MA, Schroeder TT, Huttner WB, Brakebusch C, G?tz M
Nature neuroscience9(9)1099-107(2006 Sep)

Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3.
Izaki T, Kamakura S, Kohjima M, Sumimoto H
Biochemical and biophysical research communications341(4)1001-6(2006 Mar)

Focal ischemia induces expression of protease-activated receptor1 (PAR1) and PAR3 on microglia and enhances PAR4 labeling in the penumbra.
Henrich-Noack P, Riek-Burchardt M, Baldauf K, Reiser G, Reymann KG
Brain research1070(1)232-41(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PAR 3 is regulated at the transcriptional level in a rat model of lipopolysaccharide induced acute lung injury and probably has a distinct role.
Title: Differential expression, time course and distribution of four PARs in rats with endotoxin-induced acute lung injury.

PAR1 and PAR3 could be involved in thrombin-modulated initiation of post-ischemic inflammation and PAR4 may be associated with neuronal degeneration. (Protease-activated receptor 1, 3, 4)
Title: Focal ischemia induces expression of protease-activated receptor1 (PAR1) and PAR3 on microglia and enhances PAR4 labeling in the penumbra.

Involvement of the thrombin receptors PAR-1, PAR-3, and PAR-4 in the pathophysiology of brain ischemia was suggested by decreased mRNA levels observed for PAR-1, -2, and -3, whereas PAR-4 levels were reduced only after 7 days
Title: Transient focal ischemia in rat brain differentially regulates mRNA expression of protease-activated receptors 1 to 4.

Par-3 mRNA is expressed in the developing rat eye and adult retina but shows a delayed upregulation after optic nerve crush compared to Par-1, Par-2, and Par-4.
Title: Protease-activated receptor subtype expression in developing eye and adult retina of the rat after optic nerve crush.

Expression of protease-activated receptor 3 does not change significantly in the hippocampus following severe ischemic insult.
Title: Increase of prothrombin-mRNA after global cerebral ischemia in rats, with constant expression of protease nexin-1 and protease-activated receptors.

The following F2rl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F2rl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09246	NM_053313.1 Latest version!	Rattus norvegicus coagulation factor II (thrombin) receptor-like 2 (F2rl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F2rl2 ( NM_053313.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445765.1 Rattus norvegicus coagulation factor II (thrombin) receptor-like 2 (F2rl2), mRNA.

Price & Availability↑

CloneID	ORa09246
Clone ID Related Accession (Same CDS sequence)	NM_053313.1
Accession Version	NM_053313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	proteinase-activated receptor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF310076.1. On Jun 21, 2005 this sequence version replaced XM_579551.1. ##Evidence-Data-START## Transcript exon combination :: AF310076.1, FN804688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGAGATGA AAGTCCTTAT CTTGGTTGGG GTTAGGCTGC TGTTTCTGCC GACCACTGTT 
TGCCAAAGTG GCATGAAACA TGTTTCAGAC AACTCAGCCT TAACTGCTGA GAGTTTTAAT 
GGCAACGAAC ATTCCTTTGA AGAATTCCCA CTTTCTGACA TAGAGGGCTG GACAGGAGCC 
ACCACAACTA TAAAAGCGAA GTGTCCCGAA GAAAGCATTA CAACTCTCCA TGTGAATAAT 
GCTACCATGG GATACCTGAG AAGTTCCTTA AGTACCAAAG TGATACCTGC CATCTACATC 
CTGGTGTTTG TGATTGGTGT ACCAGCGAAC ATCGTGACCC TGTGGAAACT CTCCTCAAGG 
ACCAAATCCA TCTGTCTGGT CATCTTTCAC ACCAACCTGG CCATCGCGGA TCTCCTTTTC 
TGTGTCACGC TGCCGTTTAA GATCGCCTAC CATCTCAATG GGAACGACTG GGTCTTTGGC 
GAGGTCATGT GCCGGGTCAC CACGGTCGCT TTCTACGGCA ACATGTATTG TGCTATTCTC 
ATCCTCACCT GTATGGGCAT CAACCGCTAC CTGGCCACGG TCCATCCCTT CACATACCGC 
AAGCTGCCCA AACGCAACTT CACTTTGCTC ATGTGTGGCG TGGTGTGGGT CATGGTGGTC 
TTATATATGT TGCCGTTGGC CATCCTCAAG CAGGAGTACC ATCTTGTCCA ACCAGGGATC 
ACCACCTGCC ACGACGTCCA CGACACATGC GAGTCCCCGT TGCCCTTCCA GTTCTACTAC 
TTCGTCTCCT TGGCCTTCTT TGGGTTCCTT ATCCCATTTG TGGTCAGTGT CTTCTGTTAC 
ACGACTCTCA TCCACAAGCT TAACGCGCAG GATCGCAAAT GGCTGAGGTA CATCAAGGCG 
GTTCTCCTCA TCCTTGTGAT TTTCACCATC TGCTTTGCCC CCACCAACAT CATACTCATA 
ATTCACCACG CCAACTACTA CTACAGCAAC ACTGATAGCT TGTACTTTAT GTATCTCATA 
GCTCTCTGCC TGGGCAGCCT GAATAGTTGC CTAGACCCAT TCCTTTATTT TATCATGTCA 
AAAATTGTAG ATCAGCTGAC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445765.1
CDS	63..1169
Translation	MEMKVLILVGVRLLFLPTTVCQSGMKHVSDNSALTAESFNGNEHSFEEFPLSDIEGWTGATTTIKAKCPEESITTLHVNNATMGYLRSSLSTKVIPAIYILVFVIGVPANIVTLWKLSSRTKSICLVIFHTNLAIADLLFCVTLPFKIAYHLNGNDWVFGEVMCRVTTVAFYGNMYCAILILTCMGINRYLATVHPFTYRKLPKRNFTLLMCGVVWVMVVLYMLPLAILKQEYHLVQPGITTCHDVHDTCESPLPFQFYYFVSLAFFGFLIPFVVSVFCYTTLIHKLNAQDRKWLRYIKAVLLILVIFTICFAPTNIILIIHHANYYYSNTDSLYFMYLIALCLGSLNSCLDPFLYFIMSKIVDQLTS

Target ORF information:

RefSeq Version	NM_053313.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus coagulation factor II (thrombin) receptor-like 2 (F2rl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053313.1

ORF Insert Sequence:

ATGGAGATGA AAGTCCTTAT CTTGGTTGGG GTTAGGCTGC TGTTTCTGCC GACCACTGTT 
TGCCAAAGTG GCATGAAACA TGTTTCAGAC AACTCAGCCT TAACTGCTGA GAGTTTTAAT 
GGCAACGAAC ATTCCTTTGA AGAATTCCCA CTTTCTGACA TAGAGGGCTG GACAGGAGCC 
ACCACAACTA TAAAAGCGAA GTGTCCCGAA GAAAGCATTA CAACTCTCCA TGTGAATAAT 
GCTACCATGG GATACCTGAG AAGTTCCTTA AGTACCAAAG TGATACCTGC CATCTACATC 
CTGGTGTTTG TGATTGGTGT ACCAGCGAAC ATCGTGACCC TGTGGAAACT CTCCTCAAGG 
ACCAAATCCA TCTGTCTGGT CATCTTTCAC ACCAACCTGG CCATCGCGGA TCTCCTTTTC 
TGTGTCACGC TGCCGTTTAA GATCGCCTAC CATCTCAATG GGAACGACTG GGTCTTTGGC 
GAGGTCATGT GCCGGGTCAC CACGGTCGCT TTCTACGGCA ACATGTATTG TGCTATTCTC 
ATCCTCACCT GTATGGGCAT CAACCGCTAC CTGGCCACGG TCCATCCCTT CACATACCGC 
AAGCTGCCCA AACGCAACTT CACTTTGCTC ATGTGTGGCG TGGTGTGGGT CATGGTGGTC 
TTATATATGT TGCCGTTGGC CATCCTCAAG CAGGAGTACC ATCTTGTCCA ACCAGGGATC 
ACCACCTGCC ACGACGTCCA CGACACATGC GAGTCCCCGT TGCCCTTCCA GTTCTACTAC 
TTCGTCTCCT TGGCCTTCTT TGGGTTCCTT ATCCCATTTG TGGTCAGTGT CTTCTGTTAC 
ACGACTCTCA TCCACAAGCT TAACGCGCAG GATCGCAAAT GGCTGAGGTA CATCAAGGCG 
GTTCTCCTCA TCCTTGTGAT TTTCACCATC TGCTTTGCCC CCACCAACAT CATACTCATA 
ATTCACCACG CCAACTACTA CTACAGCAAC ACTGATAGCT TGTACTTTAT GTATCTCATA 
GCTCTCTGCC TGGGCAGCCT GAATAGTTGC CTAGACCCAT TCCTTTATTT TATCATGTCA 
AAAATTGTAG ATCAGCTGAC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cross Talk Pathways Between Coagulation and Inflammation.
Circulation research118(9)1392-408(2016 Apr)
Foley JH,Conway EM

Differential expression, time course and distribution of four PARs in rats with endotoxin-induced acute lung injury.
Inflammation30(1-2)14-27(2007 Apr)
Jesmin S,Gando S,Zaedi S,Sakuraya F

The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface.
Nature neuroscience9(9)1099-107(2006 Sep)
Cappello S,Attardo A,Wu X,Iwasato T,Itohara S,Wilsch-Br?uninger M,Eilken HM,Rieger MA,Schroeder TT,Huttner WB,Brakebusch C,G?tz M

Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3.
Biochemical and biophysical research communications341(4)1001-6(2006 Mar)
Izaki T,Kamakura S,Kohjima M,Sumimoto H

Focal ischemia induces expression of protease-activated receptor1 (PAR1) and PAR3 on microglia and enhances PAR4 labeling in the penumbra.
Brain research1070(1)232-41(2006 Jan)
Henrich-Noack P,Riek-Burchardt M,Baldauf K,Reiser G,Reymann KG