Pecam1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pecam1
Entrez Gene ID	29583
Full Name	platelet and endothelial cell adhesion molecule 1
Synonyms	CD31,Pecam
General protein information	Preferred Names platelet and endothelial cell adhesion molecule 1 Names platelet endothelial cell adhesion molecule PECAM-1 platelet/endothelial cell adhesion molecule 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	cell adhesion molecule, also intracellular signaling molecule; may mediate leukocyte adhesion and migration, angiogenesis, and thrombosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031591.1	NP_113779.1	platelet endothelial cell adhesion molecule precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PECAM1	XP_004946249.1
	PECAM1	XP_001116542.1
Bos taurus (cattle)	PECAM1	NP_776996.2
Danio rerio (zebrafish)	zmp:0000000686	NP_001107271.1
Pan troglodytes (chimpanzee)	PECAM1	XP_511582.2
Canis lupus familiaris (dog)	PECAM1	XP_853419.2
Mus musculus (house mouse)	Pecam1	NP_032842.2
Rattus norvegicus (Norway rat)	Pecam1	NP_113779.1
Danio rerio (zebrafish)	si:dkey-237i9.8	XP_005166258.1
Xenopus tropicalis (tropical clawed frog)	LOC100493686	XP_002942442.2
Homo sapiens (human)	PECAM1	NP_000433.4

Gene Ontology
Bibliography

Related articles in PubMed

Structural Basis for Human PECAM-1-Mediated Trans-homophilic Cell Adhesion.
Hu M, Zhang H, Liu Q, Hao Q
Scientific reports638655(2016 Dec)

PECAM1 regulates flow-mediated Gab1 tyrosine phosphorylation and signaling.
Xu S, Ha CH, Wang W, Xu X, Yin M, Jin FQ, Mastrangelo M, Koroleva M, Fujiwara K, Jin ZG
Cellular signalling28(3)117-124(2016 Mar)

Structural basis for PECAM-1 homophilic binding.
Paddock C, Zhou D, Lertkiatmongkol P, Newman PJ, Zhu J
Blood127(8)1052-61(2016 Feb)

Platelet endothelial cell adhesion molecule-1 (PECAM1) plays a critical role in the maintenance of human vascular endothelial barrier function.
Ren Q, Ren L, Ren C, Liu X, Dong C, Zhang X
Cell biochemistry and function33(8)560-5(2015 Dec)

Platelet endothelial cell adhesion molecule-1 gene 125C/G polymorphism is associated with deep vein thrombosis.
Li G, Han ZL, Dong HG, Zhang X, Kong XQ, Jin X
Molecular medicine reports12(2)2203-10(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Circulating PECAM-1 is a promising biomarker for the detection and monitoring of ventilator-induced lung injury.
Title: Soluble platelet-endothelial cell adhesion molecule-1, a biomarker of ventilator-induced lung injury.

Shock wave therapy leads to mobilization of endogenous CD31/CD34-positive endothelial cells.
Title: Shock wave treatment induces angiogenesis and mobilizes endogenous CD31/CD34-positive endothelial cells in a hindlimb ischemia model: implications for angiogenesis and vasculogenesis.

connection of NO/IL-10/PECAM-1 during in vivo inflammatory conditions elicited by LPS has been shown
Title: Nitric oxide modulates lipopolysaccharide-induced endothelial platelet endothelial cell adhesion molecule expression via interleukin-10.

Rg1 could increase the expression of HIF1-alpha and CD31 in myocardium and stimulate angiogenesis.
Title: Effect and mechanism of panaxoside Rg1 on neovascularization in myocardial infarction rats.

These results show that NO, produced at physiological levels, controls expression of constitutive adhesion molecules L-selectin and PECAM-1 in cell membranes by different mechanisms of action.
Title: In vivo blockade of Ca(+2)-dependent nitric oxide synthases impairs expressions of L-selectin and PECAM-1.

The following Pecam1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pecam1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08193	NM_031591.1 Latest version!	Rattus norvegicus platelet and endothelial cell adhesion molecule 1 (Pecam1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$398.30 $569.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pecam1 ( NM_031591.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113779.1 Rattus norvegicus platelet and endothelial cell adhesion molecule 1 (Pecam1), mRNA.

Price & Availability↑

CloneID	ORa08193
Clone ID Related Accession (Same CDS sequence)	NM_031591.1
Accession Version	NM_031591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	platelet endothelial cell adhesion molecule precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104711.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC104711.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCTCCTGG CTCTGCTGCT CACCATGCTG CTCTATGCAA GCCTCCAGGC TCAGGAAAAC 
TCTTTCACCA TTAACAGCAT CCATATGGAA AGTCGGCCCT CCTGGGAGGT ATCGAATGGG 
CAGAAACTGA CCCTGCAGTG CCTTGTCGAT ATCAGCACCA CCTCGAAATC TAGGCCTCAG 
CACCAGGTGC TATTCTATAA GGACGATGCG CTGGTGTACA ACGTCTCCTC CAGTGAGCAC 
ACAGAGAGCT TCGTCATTCC TCAGTCTCGG GTCTTCCACG CCGGGAAGTA CAAATGCACC 
GTGATACTGA ACAGCAAGGA AAAAACCACA ATTGAGTACC AGCTGACGGT GAACGGTGTA 
CCCATGCCTG AGGTGACCGT GGACAAAAAG GAGGTGACAG AAGGTGGGAT TGTGACAGTC 
AATTGCTCCA TGCAAGAAGA AAAGCCACCA ATATATTTTA AAATTGAAAA AGTGGAACTG 
GGGACAAAGA ACGTCAAGCT AAGTAGAGAG AAGACCTCCA ACATGAACTT TGTGCTCATA 
GAATTCCCCA TCGAGGAGCA AGACCACCTG TTAGTGTTTC GCTGCCAAGC TGGCGTCCTG 
TCCGGAATCA AAATGCAGAC CTCAGAATTC ATCCGGAGTG AATACGTCAC TGTGCAGGAG 
TTCTTCTCTA CACCCAAGTT TCAAATCCAG CCTCCTGAGA TGATCATAGA AGGAAACCAG 
CTGCACATCA AGTGCAGCGT GCAGGTGGCA CACTTGGCCC AGGAATTTCC AGAAATTATA 
ATCCAGAAAG ACAAGGCGAT CGTGGCCACC TCCAAGCAAA GCAAAGAAGC TGTCTACTCA 
GTCATGGCCT TGGTAGAGCA CAGTGGACAC TACACGTGCA AAGTGGAATC GAACCGCATC 
TCCAAGGCCA GTAGCATCCT GGTCAACATA ACAGAACTGT TTCCCAGGCC GAAGCTAGAA 
CTCTCCTCCA GCCGTCTGGA CCAAGGGGAA ATGCTGGACC TGTCCTGCTC TGTCTCGGGT 
GCGCCTGTAG CTAACTTCAC CATCCAGAAG GAAGAGACGG TGTTGTCACA GTATCAGAAT 
TTCAGCAAGA TTGCCGAGGA GAGGGACAGC GGGCTGTACA GCTGTACAGC AGGCATCGGC 
AAAGTGGTCA AGAGAAGCAA TTTGGTACCC GTCCAGGTGT GCGAAATGCT CTCCAAACCC 
CGGATTTTTC ATGATGCCAA ATTTGAGATC ATAAAAGGAC AAATCATAGG TATCAGCTGC 
CAGTCAGTAA ATGGGACTGC GCCCATCACT TACCGCCTCC TAAGAGCAAA GAGCAACTTC 
CAGACCGTCC AGAAGAACTC CAATGACCCA GTGACATTCA CAGACAAGCC CACCAGAGAC 
ATGGAATACC AGTGTATAGT GGATAATTGC CACTCTCACC CCGAGGTGCG CAGCGAGATC 
CTGAGGGTCA AGGTAATAGC CCCGGTGGAT GAAGTTACGA TCTCCATCCT GTCGGGTAAC 
GATGTACAGT CTGGAGATGA AATGGTGCTT CGGTGCTCTG TGAAAGAGGG GACTGGCCCT 
GTCACGTTTC AGTTTTACAA AGAAAAGGAG GGCAGACCCT TCCACGAAGA AACCGTGAAT 
GACACCCAAG TGTTTTGGCA CCATGAACAA ACTAGCAAGG AGCAGGAAGG CCAGTACTAT 
TGTACAGCGT TCAACAGAGC CAGCATTGTG ACCAGTCTCC GAAGCGGCCC TCTAACAGTC 
AGAGTCTTTC TTGCTCCGTG GAAGAAAGGG CTCATTGCAG TCGTTGTCAT TGGAGTGGTC 
ATTGCCGCCT TGATAGTTGC AGCCAAATAC TACTTCCTGA GGAAAGCCAA GGCCAAGCAG 
AAGCCCGTGG AGATGTCCAG GCCAGCAGTC CCACTTCTGA ACTCCAACAG TGAGAAGGTT 
TCTGAGCCCA GTGTGGAAAC CAACAGCCAT TACGACTCCC AGAACATGGA TGTGGAGTAC 
ACAGAAGTGG AAGTGTCCTC CCTTGAGCCT CACCAAGAGA ACGGAAGGCT CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113779.1
CDS	196..2232
Translation	MLLALLLTMLLYASLQAQENSFTINSIHMESRPSWEVSNGQKLTLQCLVDISTTSKSRPQHQVLFYKDDALVYNVSSSEHTESFVIPQSRVFHAGKYKCTVILNSKEKTTIEYQLTVNGVPMPEVTVDKKEVTEGGIVTVNCSMQEEKPPIYFKIEKVELGTKNVKLSREKTSNMNFVLIEFPIEEQDHLLVFRCQAGVLSGIKMQTSEFIRSEYVTVQEFFSTPKFQIQPPEMIIEGNQLHIKCSVQVAHLAQEFPEIIIQKDKAIVATSKQSKEAVYSVMALVEHSGHYTCKVESNRISKASSILVNITELFPRPKLELSSSRLDQGEMLDLSCSVSGAPVANFTIQKEETVLSQYQNFSKIAEERDSGLYSCTAGIGKVVKRSNLVPVQVCEMLSKPRIFHDAKFEIIKGQIIGISCQSVNGTAPITYRLLRAKSNFQTVQKNSNDPVTFTDKPTRDMEYQCIVDNCHSHPEVRSEILRVKVIAPVDEVTISILSGNDVQSGDEMVLRCSVKEGTGPVTFQFYKEKEGRPFHEETVNDTQVFWHHEQTSKEQEGQYYCTAFNRASIVTSLRSGPLTVRVFLAPWKKGLIAVVVIGVVIAALIVAAKYYFLRKAKAKQKPVEMSRPAVPLLNSNSEKVSEPSVETNSHYDSQNMDVEYTEVEVSSLEPHQENGRLP

Target ORF information:

RefSeq Version	NM_031591.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus platelet and endothelial cell adhesion molecule 1 (Pecam1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031591.1

ORF Insert Sequence:

ATGCTCCTGG CTCTGCTGCT CACCATGCTG CTCTATGCAA GCCTCCAGGC TCAGGAAAAC 
TCTTTCACCA TTAACAGCAT CCATATGGAA AGTCGGCCCT CCTGGGAGGT ATCGAATGGG 
CAGAAACTGA CCCTGCAGTG CCTTGTCGAT ATCAGCACCA CCTCGAAATC TAGGCCTCAG 
CACCAGGTGC TATTCTATAA GGACGATGCG CTGGTGTACA ACGTCTCCTC CAGTGAGCAC 
ACAGAGAGCT TCGTCATTCC TCAGTCTCGG GTCTTCCACG CCGGGAAGTA CAAATGCACC 
GTGATACTGA ACAGCAAGGA AAAAACCACA ATTGAGTACC AGCTGACGGT GAACGGTGTA 
CCCATGCCTG AGGTGACCGT GGACAAAAAG GAGGTGACAG AAGGTGGGAT TGTGACAGTC 
AATTGCTCCA TGCAAGAAGA AAAGCCACCA ATATATTTTA AAATTGAAAA AGTGGAACTG 
GGGACAAAGA ACGTCAAGCT AAGTAGAGAG AAGACCTCCA ACATGAACTT TGTGCTCATA 
GAATTCCCCA TCGAGGAGCA AGACCACCTG TTAGTGTTTC GCTGCCAAGC TGGCGTCCTG 
TCCGGAATCA AAATGCAGAC CTCAGAATTC ATCCGGAGTG AATACGTCAC TGTGCAGGAG 
TTCTTCTCTA CACCCAAGTT TCAAATCCAG CCTCCTGAGA TGATCATAGA AGGAAACCAG 
CTGCACATCA AGTGCAGCGT GCAGGTGGCA CACTTGGCCC AGGAATTTCC AGAAATTATA 
ATCCAGAAAG ACAAGGCGAT CGTGGCCACC TCCAAGCAAA GCAAAGAAGC TGTCTACTCA 
GTCATGGCCT TGGTAGAGCA CAGTGGACAC TACACGTGCA AAGTGGAATC GAACCGCATC 
TCCAAGGCCA GTAGCATCCT GGTCAACATA ACAGAACTGT TTCCCAGGCC GAAGCTAGAA 
CTCTCCTCCA GCCGTCTGGA CCAAGGGGAA ATGCTGGACC TGTCCTGCTC TGTCTCGGGT 
GCGCCTGTAG CTAACTTCAC CATCCAGAAG GAAGAGACGG TGTTGTCACA GTATCAGAAT 
TTCAGCAAGA TTGCCGAGGA GAGGGACAGC GGGCTGTACA GCTGTACAGC AGGCATCGGC 
AAAGTGGTCA AGAGAAGCAA TTTGGTACCC GTCCAGGTGT GCGAAATGCT CTCCAAACCC 
CGGATTTTTC ATGATGCCAA ATTTGAGATC ATAAAAGGAC AAATCATAGG TATCAGCTGC 
CAGTCAGTAA ATGGGACTGC GCCCATCACT TACCGCCTCC TAAGAGCAAA GAGCAACTTC 
CAGACCGTCC AGAAGAACTC CAATGACCCA GTGACATTCA CAGACAAGCC CACCAGAGAC 
ATGGAATACC AGTGTATAGT GGATAATTGC CACTCTCACC CCGAGGTGCG CAGCGAGATC 
CTGAGGGTCA AGGTAATAGC CCCGGTGGAT GAAGTTACGA TCTCCATCCT GTCGGGTAAC 
GATGTACAGT CTGGAGATGA AATGGTGCTT CGGTGCTCTG TGAAAGAGGG GACTGGCCCT 
GTCACGTTTC AGTTTTACAA AGAAAAGGAG GGCAGACCCT TCCACGAAGA AACCGTGAAT 
GACACCCAAG TGTTTTGGCA CCATGAACAA ACTAGCAAGG AGCAGGAAGG CCAGTACTAT 
TGTACAGCGT TCAACAGAGC CAGCATTGTG ACCAGTCTCC GAAGCGGCCC TCTAACAGTC 
AGAGTCTTTC TTGCTCCGTG GAAGAAAGGG CTCATTGCAG TCGTTGTCAT TGGAGTGGTC 
ATTGCCGCCT TGATAGTTGC AGCCAAATAC TACTTCCTGA GGAAAGCCAA GGCCAAGCAG 
AAGCCCGTGG AGATGTCCAG GCCAGCAGTC CCACTTCTGA ACTCCAACAG TGAGAAGGTT 
TCTGAGCCCA GTGTGGAAAC CAACAGCCAT TACGACTCCC AGAACATGGA TGTGGAGTAC 
ACAGAAGTGG AAGTGTCCTC CCTTGAGCCT CACCAAGAGA ACGGAAGGCT CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural Basis for Human PECAM-1-Mediated Trans-homophilic Cell Adhesion.
Scientific reports638655(2016 Dec)
Hu M,Zhang H,Liu Q,Hao Q

PECAM1 regulates flow-mediated Gab1 tyrosine phosphorylation and signaling.
Cellular signalling28(3)117-124(2016 Mar)
Xu S,Ha CH,Wang W,Xu X,Yin M,Jin FQ,Mastrangelo M,Koroleva M,Fujiwara K,Jin ZG

Structural basis for PECAM-1 homophilic binding.
Blood127(8)1052-61(2016 Feb)
Paddock C,Zhou D,Lertkiatmongkol P,Newman PJ,Zhu J

Platelet endothelial cell adhesion molecule-1 (PECAM1) plays a critical role in the maintenance of human vascular endothelial barrier function.
Cell biochemistry and function33(8)560-5(2015 Dec)
Ren Q,Ren L,Ren C,Liu X,Dong C,Zhang X

Platelet endothelial cell adhesion molecule-1 gene 125C/G polymorphism is associated with deep vein thrombosis.
Molecular medicine reports12(2)2203-10(2015 Aug)
Li G,Han ZL,Dong HG,Zhang X,Kong XQ,Jin X