Hpd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hpd
Entrez Gene ID	29531
Full Name	4-hydroxyphenylpyruvate dioxygenase
General protein information	Preferred Names 4-hydroxyphenylpyruvate dioxygenase Names 4-hydroxyphenylpyruvate dioxygenase 4-hydroxyphenylpyruvic acid dioxygenase 4-hydroxyphenylpyruvic acid oxidase 4HPPD HPPDase f Alloantigen f protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	catalyzes the conversion of 4-hydroxyphenylpyruvate to homogenistate in tyrosine catabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017233.1	NP_058929.1	4-hydroxyphenylpyruvate dioxygenase

Pathways from BioSystems

Homologies

	HPD	XP_001096175.1
Mus musculus (house mouse)	Hpd	NP_032303.1
Caenorhabditis elegans (roundworm)	hpd-1	NP_499324.1
Xenopus tropicalis (tropical clawed frog)	hpd	NP_001072376.1
Bos taurus (cattle)	HPD	NP_001015611.1
Rattus norvegicus (Norway rat)	Hpd	NP_058929.1
Gallus gallus (chicken)	HPD	NP_001264420.1
Magnaporthe oryzae (rice blast fungus)	MGG_06691	XP_003709399.1
Neurospora crassa	NCU01830	XP_956614.1
Arabidopsis thaliana (thale cress)	PDS1	NP_172144.2
Homo sapiens (human)	HPD	NP_002141.1
Pan troglodytes (chimpanzee)	HPD	XP_528667.2
Drosophila melanogaster (fruit fly)	CG11796	NP_730536.1
Canis lupus familiaris (dog)	HPD	XP_853422.1
Oryza sativa (rice)	Os02g0168100	NP_001046010.1
Danio rerio (zebrafish)	hpdb	NP_001003742.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004802	XP_318007.3

Gene Ontology
Bibliography

Related articles in PubMed

A performance evaluation of three drug-induced liver injury biomarkers in the rat: alpha-glutathione S-transferase, arginase 1, and 4-hydroxyphenyl-pyruvate dioxygenase.
Bailey WJ, Holder D, Patel H, Devlin P, Gonzalez RJ, Hamilton V, Muniappa N, Hamlin DM, Thomas CE, Sistare FD, Glaab WE
Toxicological sciences : an official journal of the Society of Toxicology130(2)229-44(2012 Dec)

Product analysis and inhibition studies of a causative Asn to Ser variant of 4-hydroxyphenylpyruvate dioxygenase suggest a simple route to the treatment of Hawkinsinuria.
Brownlee JM, Heinz B, Bates J, Moran GR
Biochemistry49(33)7218-26(2010 Aug)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Structural basis for herbicidal inhibitor selectivity revealed by comparison of crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases.
Yang C, Pflugrath JW, Camper DL, Foster ML, Pernich DJ, Walsh TA
Biochemistry43(32)10414-23(2004 Aug)

Tissue distribution, intracellular localization and proteolytic processing of rat 4-hydroxyphenylpyruvate dioxygenase.
Neve S, Aarenstrup L, Tornehave D, Rahbek-Nielsen H, Corydon TJ, Roepstorff P, Kristiansen K
Cell biology international27(8)611-24(2003)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report utility of HPD for monitoring drug-induced liver injury in rat model.
Title: A performance evaluation of three drug-induced liver injury biomarkers in the rat: alpha-glutathione S-transferase, arginase 1, and 4-hydroxyphenyl-pyruvate dioxygenase.

Mutation in the rat (N241S) 4-hydroxyphenylpyruvate dioxygenase genes shows that the variant enzyme forms quinolacetic acid in place of the native product 2,5-dihydroxyphenylacetic acid.
Title: Product analysis and inhibition studies of a causative Asn to Ser variant of 4-hydroxyphenylpyruvate dioxygenase suggest a simple route to the treatment of Hawkinsinuria.

The following Hpd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hpd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03331	NM_017233.1 Latest version!	Rattus norvegicus 4-hydroxyphenylpyruvate dioxygenase (Hpd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hpd ( NM_017233.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058929.1 Rattus norvegicus 4-hydroxyphenylpyruvate dioxygenase (Hpd), mRNA.

Price & Availability↑

CloneID	ORa03331
Clone ID Related Accession (Same CDS sequence)	NM_017233.1
Accession Version	NM_017233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	4-hydroxyphenylpyruvate dioxygenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF082834.1. ##Evidence-Data-START## Transcript exon combination :: AF082834.1, FQ210905.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689598 [ECO:0000348] ##Evidence-Data-END##

ATGACAACCT ACAGCAACAA AGGACCAAAG CCTGAGAGAG GCCGGTTCCT CCATTTCCAC 
TCTGTGACCT TCTGGGTTGG CAACGCCAAG CAGGCTGCCT CCTTCTATTG CAACAAGATG 
GGCTTCGAAC CGCTGGCCTA CAAGGGCCTG GAGACGGGCT CCCGGGAGGT GGTCAGTCAT 
GTCATCAAGC AAGGGAAAAT TGTGTTTGTT CTCTGCTCTG CTCTCAATCC CTGGAACAAA 
GAGATGGGTG ACCACCTGGT GAAGCATGGT GATGGCGTAA AGGACATCGC ATTCGAGGTG 
GAAGACTGTG AACACATTGT GCAGAAAGCC CGAGAACGGG GCGCTAAAAT TGTACGGGAG 
CCATGGGTGG AGGAAGACAA ATTCGGGAAG GTGAAGTTCG CTGTGCTGCA GACGTATGGA 
GATACCACGC ACACCCTGGT GGAGAAGATC AACTACACCG GTCGTTTCTT ACCTGGATTC 
GAGGCCCCAA CATACAAGGA CACCCTACTT CCAAAACTAC CCAGCTGTAA CCTGGAGATC 
ATCGACCATA TTGTAGGCAA CCAGCCCGAC CAGGAAATGG AGTCTGCCTC AGAATGGTAC 
CTGAAAAACC TGCAGTTCCA CCGGTTCTGG TCTGTAGACG ACACACAGGT GCACACGGAG 
TATAGCTCTC TGCGCTCCAT CGTGGTGGCC AACTATGAGG AGTCCATCAA AATGCCCATT 
AATGAACCGG CCCCGGGCAG GAAGAAGTCT CAGATCCAGG AATATGTGGA CTATAATGGG 
GGTGCTGGGG TCCAGCACAT CGCTCTCAGG ACCGAAGACA TCATCACAAC GATCCGCCAC 
TTGAGGGAAC GAGGCATGGA GTTCTTGGCT GTCCCGTCTT CTTACTACAG ACTGCTTCGG 
GAGAATCTCA AGACCTCCAA GATCCAAGTG AAGGAGAACA TGGATGTCTT GGAGGAGCTA 
AAAATCCTGG TAGACTACGA TGAGAAAGGC TACCTCCTAC AGATCTTCAC CAAGCCCATG 
CAGGACCGGC CCACGCTCTT CTTGGAAGTC ATCCAACGCC ACAACCACCA GGGCTTTGGA 
GCAGGTAACT TCAACTCTCT GTTCAAAGCT TTCGAAGAGG AGCAAGCCCT ACGGGGTAAC 
CTCACTGACC TGGAGACCAA CGGTGTGAGG TCTGGAATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058929.1
CDS	37..1218
Translation	MTTYSNKGPKPERGRFLHFHSVTFWVGNAKQAASFYCNKMGFEPLAYKGLETGSREVVSHVIKQGKIVFVLCSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCEHIVQKARERGAKIVREPWVEEDKFGKVKFAVLQTYGDTTHTLVEKINYTGRFLPGFEAPTYKDTLLPKLPSCNLEIIDHIVGNQPDQEMESASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGRKKSQIQEYVDYNGGAGVQHIALRTEDIITTIRHLRERGMEFLAVPSSYYRLLRENLKTSKIQVKENMDVLEELKILVDYDEKGYLLQIFTKPMQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQALRGNLTDLETNGVRSGM

Target ORF information:

RefSeq Version	NM_017233.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 4-hydroxyphenylpyruvate dioxygenase (Hpd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017233.1

ORF Insert Sequence:

ATGACAACCT ACAGCAACAA AGGACCAAAG CCTGAGAGAG GCCGGTTCCT CCATTTCCAC 
TCTGTGACCT TCTGGGTTGG CAACGCCAAG CAGGCTGCCT CCTTCTATTG CAACAAGATG 
GGCTTCGAAC CGCTGGCCTA CAAGGGCCTG GAGACGGGCT CCCGGGAGGT GGTCAGTCAT 
GTCATCAAGC AAGGGAAAAT TGTGTTTGTT CTCTGCTCTG CTCTCAATCC CTGGAACAAA 
GAGATGGGTG ACCACCTGGT GAAGCATGGT GATGGCGTAA AGGACATCGC ATTCGAGGTG 
GAAGACTGTG AACACATTGT GCAGAAAGCC CGAGAACGGG GCGCTAAAAT TGTACGGGAG 
CCATGGGTGG AGGAAGACAA ATTCGGGAAG GTGAAGTTCG CTGTGCTGCA GACGTATGGA 
GATACCACGC ACACCCTGGT GGAGAAGATC AACTACACCG GTCGTTTCTT ACCTGGATTC 
GAGGCCCCAA CATACAAGGA CACCCTACTT CCAAAACTAC CCAGCTGTAA CCTGGAGATC 
ATCGACCATA TTGTAGGCAA CCAGCCCGAC CAGGAAATGG AGTCTGCCTC AGAATGGTAC 
CTGAAAAACC TGCAGTTCCA CCGGTTCTGG TCTGTAGACG ACACACAGGT GCACACGGAG 
TATAGCTCTC TGCGCTCCAT CGTGGTGGCC AACTATGAGG AGTCCATCAA AATGCCCATT 
AATGAACCGG CCCCGGGCAG GAAGAAGTCT CAGATCCAGG AATATGTGGA CTATAATGGG 
GGTGCTGGGG TCCAGCACAT CGCTCTCAGG ACCGAAGACA TCATCACAAC GATCCGCCAC 
TTGAGGGAAC GAGGCATGGA GTTCTTGGCT GTCCCGTCTT CTTACTACAG ACTGCTTCGG 
GAGAATCTCA AGACCTCCAA GATCCAAGTG AAGGAGAACA TGGATGTCTT GGAGGAGCTA 
AAAATCCTGG TAGACTACGA TGAGAAAGGC TACCTCCTAC AGATCTTCAC CAAGCCCATG 
CAGGACCGGC CCACGCTCTT CTTGGAAGTC ATCCAACGCC ACAACCACCA GGGCTTTGGA 
GCAGGTAACT TCAACTCTCT GTTCAAAGCT TTCGAAGAGG AGCAAGCCCT ACGGGGTAAC 
CTCACTGACC TGGAGACCAA CGGTGTGAGG TCTGGAATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A performance evaluation of three drug-induced liver injury biomarkers in the rat: alpha-glutathione S-transferase, arginase 1, and 4-hydroxyphenyl-pyruvate dioxygenase.
Toxicological sciences : an official journal of the Society of Toxicology130(2)229-44(2012 Dec)
Bailey WJ,Holder D,Patel H,Devlin P,Gonzalez RJ,Hamilton V,Muniappa N,Hamlin DM,Thomas CE,Sistare FD,Glaab WE

Product analysis and inhibition studies of a causative Asn to Ser variant of 4-hydroxyphenylpyruvate dioxygenase suggest a simple route to the treatment of Hawkinsinuria.
Biochemistry49(33)7218-26(2010 Aug)
Brownlee JM,Heinz B,Bates J,Moran GR

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Structural basis for herbicidal inhibitor selectivity revealed by comparison of crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases.
Biochemistry43(32)10414-23(2004 Aug)
Yang C,Pflugrath JW,Camper DL,Foster ML,Pernich DJ,Walsh TA

Tissue distribution, intracellular localization and proteolytic processing of rat 4-hydroxyphenylpyruvate dioxygenase.
Cell biology international27(8)611-24(2003)
Neve S,Aarenstrup L,Tornehave D,Rahbek-Nielsen H,Corydon TJ,Roepstorff P,Kristiansen K