Scamp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scamp1
Entrez Gene ID	29521
Full Name	secretory carrier membrane protein 1
General protein information	Preferred Names secretory carrier membrane protein 1 Names secretory carrier-associated membrane protein 1 SCAMP 37
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	plays a role in endocytosis; may recruit clathrin coats to the plasma membrane and to the trans-Golgi network [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100636.1	NP_001094106.1	secretory carrier-associated membrane protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCAMP1	NP_004857.4
Bos taurus (cattle)	SCAMP1	NP_001069522.1
Caenorhabditis elegans (roundworm)	scm-1	NP_490911.1
Rattus norvegicus (Norway rat)	Scamp1	NP_001094106.1
Pan troglodytes (chimpanzee)	SCAMP1	XP_517688.2
Danio rerio (zebrafish)	scamp1	NP_991255.1
Xenopus tropicalis (tropical clawed frog)	scamp1	NP_001017190.1
Canis lupus familiaris (dog)	SCAMP1	XP_859726.2
Mus musculus (house mouse)	Scamp1	NP_083429.1
Gallus gallus (chicken)	SCAMP1	NP_001264922.1
Drosophila melanogaster (fruit fly)	Scamp	NP_001259572.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002922	XP_311983.4

Gene Ontology
Bibliography

Related articles in PubMed

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kr?hnert K, Sch?fer C, Rammner B, Koo SJ, Cla?en GA, Krauss M, Haucke V, Urlaub H, Rizzoli SO
Science (New York, N.Y.)344(6187)1023-8(2014 May)

Regulation of fusion pore closure and compound exocytosis in neuroendocrine PC12 cells by SCAMP1.
Zhang J, Castle D
Traffic (Copenhagen, Denmark)12(5)600-14(2011 May)

Interaction assays in yeast and cultured cells confirm known and identify novel partners of the synaptic vesicle protein synaptophysin.
Felkl M, Leube RE
Neuroscience156(2)344-52(2008 Oct)

Nonredundant function of secretory carrier membrane protein isoforms in dense core vesicle exocytosis.
Liao H, Zhang J, Shestopal S, Szabo G, Castle A, Castle D
American journal of physiology. Cell physiology294(3)C797-809(2008 Mar)

Ubiquitously expressed secretory carrier membrane proteins (SCAMPs) 1-4 mark different pathways and exhibit limited constitutive trafficking to and from the cell surface.
Castle A, Castle D
Journal of cell science118(Pt 16)3769-80(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that SCAMP1's apparent dual role in facilitating dilation and closure of fusion pores implicates its function in exo-endocytic coupling and in the regulation of partial secretion.
Title: Regulation of fusion pore closure and compound exocytosis in neuroendocrine PC12 cells by SCAMP1.

SCAMP isoforms regulate membrane fusion and dense core vesicle exocytosis in neurons.
Title: Nonredundant function of secretory carrier membrane protein isoforms in dense core vesicle exocytosis.

SCAMPs travel between the cell surface and internal recycling systems, and form a mosaic with individual isoforms marking distinct pathways and functioning as trafficking machinery at sites of vesicle formation and fusion.
Title: Ubiquitously expressed secretory carrier membrane proteins (SCAMPs) 1-4 mark different pathways and exhibit limited constitutive trafficking to and from the cell surface.

The following Scamp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scamp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04232	NM_001100636.1 Latest version!	Rattus norvegicus secretory carrier membrane protein 1 (Scamp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scamp1 ( NM_001100636.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094106.1 Rattus norvegicus secretory carrier membrane protein 1 (Scamp1), mRNA.

Price & Availability↑

CloneID	ORa04232
Clone ID Related Accession (Same CDS sequence)	NM_001100636.1
Accession Version	NM_001100636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	secretory carrier-associated membrane protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB740599.1, L22079.1 and BC090322.1. On or before Oct 28, 2007 this sequence version replaced XM_342174.3, XM_001059643.1, XM_001059698.1, XM_001059581.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L22079.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGATT TTGACAGCAA CCCCTTTGCG GATCCGGATC TCAACAACCC GTTCAAGGAC 
CCATCTGTCA CACAGGTGAC AAGAAATGTG CCACCGGGAC TTGATGAATA TAATCCTTTC 
TCTGATTCTA GAACGCCTCC ACCAGGTGGT GTGAAAATGC CTAATGTACC CAACACCCAG 
CCAGCAATAA TGAAGCCGAC CGAGGAGCAT CCGGCTTACA CACAGATCAC AAAGGAGCAT 
GCCTTGGCTC AAGCCGAGCT TCTCAAGCGC CAAGAGGAGC TGGAGAGAAA AGCTGCAGAG 
TTAGATCGTC GAGAGCGAGA GATGCAGAAC CTGAGTCAGC ACGGGAGAAA AAATAATTGG 
CCACCTCTTC CTAGCAATTT CCCTGTGGGG CCTTGTTTCT ATCAAGACTT TTCTGTAGAC 
ATTCCCGTAG AGTTCCAGAA GACAGTGAAG CTTATGTACT ACCTATGGAT GTTCCATGCG 
GTAACCCTGT TCCTGAACAT CTTCGGATGC CTGGCTTGGT TTTGTGTTGA TTCCTCAAGA 
GCAGTTGACT TTGGACTCAG CATCCTGTGG TTCTTGCTCT TTACCCCCTG TTCATTTGTC 
TGTTGGTACA GGCCACTCTA TGGGGCCTTC AGGAGTGACA GTTCCTTCAG GTTCTTTGTG 
TTCTTCTTCG TCTATATTTG TCAGTTTGCT GTACATGTGC TCCAGGCTGC AGGATTTCAT 
AACTGGGGAA ACTGTGGTTG GATCTCATCC CTCACTGGTC TCAACAAGAA CATTCCGGTC 
GGGATCATGA TGATCATCAT CGCAGCGCTC TTTACAGCGT CCGCTGTCAT CTCGCTCGTC 
ATGTTTAAAA AAGTGCATGG ACTGTATCGC ACAACAGGTG CTAGCTTTGA GAAGGCCCAG 
CAAGAGTTTG CAACGGGAGT GATGTCCAAT AAAACTGTTC AGACAGCAGC TGCAAATGCC 
GCGTCCACCG CAGCAACCAG CGCGGCGCAG AATGCCTTCA AGGGTAACCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094106.1
CDS	110..1126
Translation	MSDFDSNPFADPDLNNPFKDPSVTQVTRNVPPGLDEYNPFSDSRTPPPGGVKMPNVPNTQPAIMKPTEEHPAYTQITKEHALAQAELLKRQEELERKAAELDRREREMQNLSQHGRKNNWPPLPSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYLWMFHAVTLFLNIFGCLAWFCVDSSRAVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAVHVLQAAGFHNWGNCGWISSLTGLNKNIPVGIMMIIIAALFTASAVISLVMFKKVHGLYRTTGASFEKAQQEFATGVMSNKTVQTAAANAASTAATSAAQNAFKGNQM

Target ORF information:

RefSeq Version	NM_001100636.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus secretory carrier membrane protein 1 (Scamp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100636.1

ORF Insert Sequence:

ATGTCGGATT TTGACAGCAA CCCCTTTGCG GATCCGGATC TCAACAACCC GTTCAAGGAC 
CCATCTGTCA CACAGGTGAC AAGAAATGTG CCACCGGGAC TTGATGAATA TAATCCTTTC 
TCTGATTCTA GAACGCCTCC ACCAGGTGGT GTGAAAATGC CTAATGTACC CAACACCCAG 
CCAGCAATAA TGAAGCCGAC CGAGGAGCAT CCGGCTTACA CACAGATCAC AAAGGAGCAT 
GCCTTGGCTC AAGCCGAGCT TCTCAAGCGC CAAGAGGAGC TGGAGAGAAA AGCTGCAGAG 
TTAGATCGTC GAGAGCGAGA GATGCAGAAC CTGAGTCAGC ACGGGAGAAA AAATAATTGG 
CCACCTCTTC CTAGCAATTT CCCTGTGGGG CCTTGTTTCT ATCAAGACTT TTCTGTAGAC 
ATTCCCGTAG AGTTCCAGAA GACAGTGAAG CTTATGTACT ACCTATGGAT GTTCCATGCG 
GTAACCCTGT TCCTGAACAT CTTCGGATGC CTGGCTTGGT TTTGTGTTGA TTCCTCAAGA 
GCAGTTGACT TTGGACTCAG CATCCTGTGG TTCTTGCTCT TTACCCCCTG TTCATTTGTC 
TGTTGGTACA GGCCACTCTA TGGGGCCTTC AGGAGTGACA GTTCCTTCAG GTTCTTTGTG 
TTCTTCTTCG TCTATATTTG TCAGTTTGCT GTACATGTGC TCCAGGCTGC AGGATTTCAT 
AACTGGGGAA ACTGTGGTTG GATCTCATCC CTCACTGGTC TCAACAAGAA CATTCCGGTC 
GGGATCATGA TGATCATCAT CGCAGCGCTC TTTACAGCGT CCGCTGTCAT CTCGCTCGTC 
ATGTTTAAAA AAGTGCATGG ACTGTATCGC ACAACAGGTG CTAGCTTTGA GAAGGCCCAG 
CAAGAGTTTG CAACGGGAGT GATGTCCAAT AAAACTGTTC AGACAGCAGC TGCAAATGCC 
GCGTCCACCG CAGCAACCAG CGCGGCGCAG AATGCCTTCA AGGGTAACCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Science (New York, N.Y.)344(6187)1023-8(2014 May)
Wilhelm BG,Mandad S,Truckenbrodt S,Kr?hnert K,Sch?fer C,Rammner B,Koo SJ,Cla?en GA,Krauss M,Haucke V,Urlaub H,Rizzoli SO

Regulation of fusion pore closure and compound exocytosis in neuroendocrine PC12 cells by SCAMP1.
Traffic (Copenhagen, Denmark)12(5)600-14(2011 May)
Zhang J,Castle D

Interaction assays in yeast and cultured cells confirm known and identify novel partners of the synaptic vesicle protein synaptophysin.
Neuroscience156(2)344-52(2008 Oct)
Felkl M,Leube RE

Nonredundant function of secretory carrier membrane protein isoforms in dense core vesicle exocytosis.
American journal of physiology. Cell physiology294(3)C797-809(2008 Mar)
Liao H,Zhang J,Shestopal S,Szabo G,Castle A,Castle D

Ubiquitously expressed secretory carrier membrane proteins (SCAMPs) 1-4 mark different pathways and exhibit limited constitutive trafficking to and from the cell surface.
Journal of cell science118(Pt 16)3769-80(2005 Aug)
Castle A,Castle D