Slc10a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc10a2
Entrez Gene ID	29500
Full Name	solute carrier family 10 member 2
Synonyms	ASBT,IBAT,ISBAT,ISBT
General protein information	Preferred Names solute carrier family 10 member 2 Names ileal sodium/bile acid cotransporter Na(+)-dependent ileal bile acid transporter apical sodium-dependent bile acid transporter ileal Na(+)/bile acid cotransporter ileal sodium-dependent bile acid transporter sodium/taurocholate-cotransporting polypeptide, ileal solute carrier family 10 (sodium/bile acid cotransporter family), member 2 solute carrier family 10 (sodium/bile acid cotransporter), member 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5
Summary	mediates sodium-dependent transport of bile acids; plays a role in bile acid circulation and reabsorption; facilitates cholesterol catabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017600053.1	XP_017455542.1	ileal sodium/bile acid cotransporter isoform X1
NM_017222.2	NP_058918.1	ileal sodium/bile acid cotransporter

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SLC10A2	XP_425589.3
Danio rerio (zebrafish)	slc10a2	NP_956652.1
Drosophila melanogaster (fruit fly)	CG9903	NP_573132.1
Xenopus tropicalis (tropical clawed frog)	LOC100487141	XP_002933069.1
Homo sapiens (human)	SLC10A2	NP_000443.1
Mus musculus (house mouse)	Slc10a2	NP_035518.1
Arabidopsis thaliana (thale cress)	AT2G26900	NP_850089.1
Canis lupus familiaris (dog)	SLC10A2	NP_001002968.1
Rattus norvegicus (Norway rat)	Slc10a2	NP_058918.1
Pan troglodytes (chimpanzee)	SLC10A2	XP_522716.2
	SLC10A2	XP_001095212.1
Bos taurus (cattle)	SLC10A2	XP_002692028.2
Oryza sativa (rice)	Os01g0645200	NP_001043701.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered expression of the Olr59, Ethe1, and Slc10a2 genes in the liver of F344 rats by neonatal thyroid hormone disruption.
Matsubara K, Nakamura N, Sanoh S, Ohta S, Kitamura S, Uramaru N, Miyagawa S, Iguchi T, Fujimoto N
Journal of applied toxicology : JAT37(9)1030-1035(2017 Sep)

Diet1 functions in the FGF15/19 enterohepatic signaling axis to modulate bile acid and lipid levels.
Vergnes L, Lee JM, Chin RG, Auwerx J, Reue K
Cell metabolism17(6)916-28(2013 Jun)

Disruption of Stard10 gene alters the PPAR?-mediated bile acid homeostasis.
Ito M, Yamanashi Y, Toyoda Y, Izumi-Nakaseko H, Oda S, Sugiyama A, Kuroda M, Suzuki H, Takada T, Adachi-Akahane S
Biochimica et biophysica acta1831(2)459-68(2013 Feb)

Impact of lactobacilli on orally acquired listeriosis.
Archambaud C, Nahori MA, Soubigou G, B?cavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)

Cytoprotective properties of rifampicin are related to the regulation of detoxification system and bile acid transporter expression during hepatocellular injury induced by hydrophobic bile acids.
Gonz?lez R, Cruz A, Ferr?n G, L?pez-Cillero P, Brice?o J, G?mez MA, Rufi?n S, Padillo J, De la Mata M, Marin JJ, Muntan? J
Journal of hepato-biliary-pancreatic sciences18(5)740-50(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The beneficial effect of rifampicin in cholestasis is associated with an increase in DME expression involved in toxic, bile acid and cholesterol metabolism, as well as a reduction in the bile acid importing system in hepatocytes.
Title: Cytoprotective properties of rifampicin are related to the regulation of detoxification system and bile acid transporter expression during hepatocellular injury induced by hydrophobic bile acids.

These data confirm that bile acids and upregulation of ASBT play a crucial role in NEC pathogenesis and suggest that inhibition of ASBT could be utilized as a therapeutic modality against this disease.
Title: Apical sodium-dependent bile acid transporter upregulation is associated with necrotizing enterocolitis.

Orthotopic transplantation of epithelial organoids containing ileal stem cells was used to generate a neoileal mucosa that expressed all 4 intestinal lineages along with a zone of active bile acid uptake and IBAT expression in a recipient segment [IBAT].
Title: Orthotopic transplantation of intestinal mucosal organoids in rodents.

ASBT gene expression is activated by 1,25(OH)(2)D(3) by specific binding to the VDRE and that such activation enhances ileal bile acid transport.
Title: Transactivation of rat apical sodium-dependent bile acid transporter and increased bile acid transport by 1alpha,25-dihydroxyvitamin D3 via the vitamin D receptor.

secretin stimulates cholehepatic shunting of conjugated bile acids and is associated with increased cholangiocyte apical membrane ASBT
Title: Secretin activation of the apical Na+-dependent bile acid transporter is associated with cholehepatic shunting in rats.

The following Slc10a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc10a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03288	NM_017222.2 Latest version!	Rattus norvegicus solute carrier family 10 member 2 (Slc10a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa03288	XM_017600053.1 Latest version!	Rattus norvegicus solute carrier family 10 (sodium/bile acid cotransporter), member 2 (Slc10a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc10a2 ( NM_017222.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058918.1 Rattus norvegicus solute carrier family 10 member 2 (Slc10a2), mRNA.

Price & Availability↑

CloneID	ORa03288
Clone ID Related Accession (Same CDS sequence)	XM_017600053.1 , NM_017222.2
Accession Version	NM_017222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	ileal sodium/bile acid cotransporter
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U07183.2. On or before Dec 17, 2003 this sequence version replaced XM_346494.1, NM_017222.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U07183.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689597 [ECO:0000350] ##Evidence-Data-END##

ATGGATAACT CCTCCGTCTG TTCCCCAAAT GCAACTTTCT GCGAAGGTGA TTCCTGCCTA 
GTAACTGAAA GCAACTTCAA TGCCATTCTC AGCACAGTGA TGAGCACCGT GCTCACCATT 
CTTCTAGCCA TGGTGATGTT TTCTATGGGG TGCAATGTGG AAATCAACAA GTTCCTAGGA 
CACATAAAGC GGCCATGGGG CATCTTCGTG GGCTTCCTCT GTCAGTTTGG AATCATGCCT 
CTCACAGGAT TTATCCTGTC TGTGGCCTCT GGCATCCTTC CTGTGCAGGC TGTGGTGGTG 
CTAATTATGG GTTGCTGCCC TGGAGGAACT GGCTCCAATA TCCTGGCCTA TTGGATAGAT 
GGTGACATGG ACCTCAGTGT TAGCATGACC ACTTGCTCCA CACTGCTTGC TCTTGGAATG 
ATGCCCCTTT GCCTCTTCAT CTATACCAAG ATGTGGGTTG ACTCAGGAAC GATTGTGATC 
CCCTACGATA GCATTGGCAT TTCTCTGGTT GCGCTTGTTA TTCCTGTTTC CATTGGAATG 
TTTGTAAATC ACAAATGGCC CCAAAAAGCG AAGATTATAC TTAAAATTGG ATCCATCGCA 
GGTGCAATTC TCATTGTGCT CATAGCTGTG GTTGGAGGAA TACTGTACCA AAGTGCCTGG 
ATCATTGAAC CCAAACTATG GATTATAGGA ACAATATTTC CTATAGCTGG CTACAGCCTT 
GGTTTCTTCC TGGCTAGACT AGCTGGTCAA CCCTGGTACA GGTGCCGAAC AGTTGCCTTG 
GAAACTGGAA TGCAGAACAC TCAACTGTGT TCCACCATTG TACAACTCTC CTTTAGCCCT 
GAGGATCTCA ACCTTGTGTT CACCTTCCCA CTCATCTATA CTGTTTTCCA GCTCGTCTTT 
GCAGCAATAA TATTAGGAAT GTATGTCACA TACAAGAAAT GTCATGGAAA AAATGATGCT 
GAGTTTCTAG AGAAAACAGA CAATGATATG GACCCCATGC CATCATTCCA GGAGACAAAC 
AAGGGATTTC AACCAGATGA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058918.1
CDS	108..1154
Translation	MDNSSVCSPNATFCEGDSCLVTESNFNAILSTVMSTVLTILLAMVMFSMGCNVEINKFLGHIKRPWGIFVGFLCQFGIMPLTGFILSVASGILPVQAVVVLIMGCCPGGTGSNILAYWIDGDMDLSVSMTTCSTLLALGMMPLCLFIYTKMWVDSGTIVIPYDSIGISLVALVIPVSIGMFVNHKWPQKAKIILKIGSIAGAILIVLIAVVGGILYQSAWIIEPKLWIIGTIFPIAGYSLGFFLARLAGQPWYRCRTVALETGMQNTQLCSTIVQLSFSPEDLNLVFTFPLIYTVFQLVFAAIILGMYVTYKKCHGKNDAEFLEKTDNDMDPMPSFQETNKGFQPDEK

Target ORF information:

RefSeq Version	NM_017222.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 10 member 2 (Slc10a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017222.2

ORF Insert Sequence:

ATGGATAACT CCTCCGTCTG TTCCCCAAAT GCAACTTTCT GCGAAGGTGA TTCCTGCCTA 
GTAACTGAAA GCAACTTCAA TGCCATTCTC AGCACAGTGA TGAGCACCGT GCTCACCATT 
CTTCTAGCCA TGGTGATGTT TTCTATGGGG TGCAATGTGG AAATCAACAA GTTCCTAGGA 
CACATAAAGC GGCCATGGGG CATCTTCGTG GGCTTCCTCT GTCAGTTTGG AATCATGCCT 
CTCACAGGAT TTATCCTGTC TGTGGCCTCT GGCATCCTTC CTGTGCAGGC TGTGGTGGTG 
CTAATTATGG GTTGCTGCCC TGGAGGAACT GGCTCCAATA TCCTGGCCTA TTGGATAGAT 
GGTGACATGG ACCTCAGTGT TAGCATGACC ACTTGCTCCA CACTGCTTGC TCTTGGAATG 
ATGCCCCTTT GCCTCTTCAT CTATACCAAG ATGTGGGTTG ACTCAGGAAC GATTGTGATC 
CCCTACGATA GCATTGGCAT TTCTCTGGTT GCGCTTGTTA TTCCTGTTTC CATTGGAATG 
TTTGTAAATC ACAAATGGCC CCAAAAAGCG AAGATTATAC TTAAAATTGG ATCCATCGCA 
GGTGCAATTC TCATTGTGCT CATAGCTGTG GTTGGAGGAA TACTGTACCA AAGTGCCTGG 
ATCATTGAAC CCAAACTATG GATTATAGGA ACAATATTTC CTATAGCTGG CTACAGCCTT 
GGTTTCTTCC TGGCTAGACT AGCTGGTCAA CCCTGGTACA GGTGCCGAAC AGTTGCCTTG 
GAAACTGGAA TGCAGAACAC TCAACTGTGT TCCACCATTG TACAACTCTC CTTTAGCCCT 
GAGGATCTCA ACCTTGTGTT CACCTTCCCA CTCATCTATA CTGTTTTCCA GCTCGTCTTT 
GCAGCAATAA TATTAGGAAT GTATGTCACA TACAAGAAAT GTCATGGAAA AAATGATGCT 
GAGTTTCTAG AGAAAACAGA CAATGATATG GACCCCATGC CATCATTCCA GGAGACAAAC 
AAGGGATTTC AACCAGATGA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc10a2 ( XM_017600053.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455542.1 Rattus norvegicus solute carrier family 10 (sodium/bile acid cotransporter), member 2 (Slc10a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa03288
Clone ID Related Accession (Same CDS sequence)	XM_017600053.1 , NM_017222.2
Accession Version	XM_017600053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ileal sodium/bile acid cotransporter isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) and transcript sequence (U07183.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 373 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGATAACT CCTCCGTCTG TTCCCCAAAT GCAACTTTCT GCGAAGGTGA TTCCTGCCTA 
GTAACTGAAA GCAACTTCAA TGCCATTCTC AGCACAGTGA TGAGCACCGT GCTCACCATT 
CTTCTAGCCA TGGTGATGTT TTCTATGGGG TGCAATGTGG AAATCAACAA GTTCCTAGGA 
CACATAAAGC GGCCATGGGG CATCTTCGTG GGCTTCCTCT GTCAGTTTGG AATCATGCCT 
CTCACAGGAT TTATCCTGTC TGTGGCCTCT GGCATCCTTC CTGTGCAGGC TGTGGTGGTG 
CTAATTATGG GTTGCTGCCC TGGAGGAACT GGCTCCAATA TCCTGGCCTA TTGGATAGAT 
GGTGACATGG ACCTCAGTGT TAGCATGACC ACTTGCTCCA CACTGCTTGC TCTTGGAATG 
ATGCCCCTTT GCCTCTTCAT CTATACCAAG ATGTGGGTTG ACTCAGGAAC GATTGTGATC 
CCCTACGATA GCATTGGCAT TTCTCTGGTT GCGCTTGTTA TTCCTGTTTC CATTGGAATG 
TTTGTAAATC ACAAATGGCC CCAAAAAGCG AAGATTATAC TTAAAATTGG ATCCATCGCA 
GGTGCAATTC TCATTGTGCT CATAGCTGTG GTTGGAGGAA TACTGTACCA AAGTGCCTGG 
ATCATTGAAC CCAAACTATG GATTATAGGA ACAATATTTC CTATAGCTGG CTACAGCCTT 
GGTTTCTTCC TGGCTAGACT AGCTGGTCAA CCCTGGTACA GGTGCCGAAC AGTTGCCTTG 
GAAACTGGAA TGCAGAACAC TCAACTGTGT TCCACCATTG TACAACTCTC CTTTAGCCCT 
GAGGATCTCA ACCTTGTGTT CACCTTCCCA CTCATCTATA CTGTTTTCCA GCTCGTCTTT 
GCAGCAATAA TATTAGGAAT GTATGTCACA TACAAGAAAT GTCATGGAAA AAATGATGCT 
GAGTTTCTAG AGAAAACAGA CAATGATATG GACCCCATGC CATCATTCCA GGAGACAAAC 
AAGGGATTTC AACCAGATGA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455542.1
CDS	885..1931
Translation	MDNSSVCSPNATFCEGDSCLVTESNFNAILSTVMSTVLTILLAMVMFSMGCNVEINKFLGHIKRPWGIFVGFLCQFGIMPLTGFILSVASGILPVQAVVVLIMGCCPGGTGSNILAYWIDGDMDLSVSMTTCSTLLALGMMPLCLFIYTKMWVDSGTIVIPYDSIGISLVALVIPVSIGMFVNHKWPQKAKIILKIGSIAGAILIVLIAVVGGILYQSAWIIEPKLWIIGTIFPIAGYSLGFFLARLAGQPWYRCRTVALETGMQNTQLCSTIVQLSFSPEDLNLVFTFPLIYTVFQLVFAAIILGMYVTYKKCHGKNDAEFLEKTDNDMDPMPSFQETNKGFQPDEK

Target ORF information:

RefSeq Version	XM_017600053.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 10 (sodium/bile acid cotransporter), member 2 (Slc10a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600053.1

ORF Insert Sequence:

ATGGATAACT CCTCCGTCTG TTCCCCAAAT GCAACTTTCT GCGAAGGTGA TTCCTGCCTA 
GTAACTGAAA GCAACTTCAA TGCCATTCTC AGCACAGTGA TGAGCACCGT GCTCACCATT 
CTTCTAGCCA TGGTGATGTT TTCTATGGGG TGCAATGTGG AAATCAACAA GTTCCTAGGA 
CACATAAAGC GGCCATGGGG CATCTTCGTG GGCTTCCTCT GTCAGTTTGG AATCATGCCT 
CTCACAGGAT TTATCCTGTC TGTGGCCTCT GGCATCCTTC CTGTGCAGGC TGTGGTGGTG 
CTAATTATGG GTTGCTGCCC TGGAGGAACT GGCTCCAATA TCCTGGCCTA TTGGATAGAT 
GGTGACATGG ACCTCAGTGT TAGCATGACC ACTTGCTCCA CACTGCTTGC TCTTGGAATG 
ATGCCCCTTT GCCTCTTCAT CTATACCAAG ATGTGGGTTG ACTCAGGAAC GATTGTGATC 
CCCTACGATA GCATTGGCAT TTCTCTGGTT GCGCTTGTTA TTCCTGTTTC CATTGGAATG 
TTTGTAAATC ACAAATGGCC CCAAAAAGCG AAGATTATAC TTAAAATTGG ATCCATCGCA 
GGTGCAATTC TCATTGTGCT CATAGCTGTG GTTGGAGGAA TACTGTACCA AAGTGCCTGG 
ATCATTGAAC CCAAACTATG GATTATAGGA ACAATATTTC CTATAGCTGG CTACAGCCTT 
GGTTTCTTCC TGGCTAGACT AGCTGGTCAA CCCTGGTACA GGTGCCGAAC AGTTGCCTTG 
GAAACTGGAA TGCAGAACAC TCAACTGTGT TCCACCATTG TACAACTCTC CTTTAGCCCT 
GAGGATCTCA ACCTTGTGTT CACCTTCCCA CTCATCTATA CTGTTTTCCA GCTCGTCTTT 
GCAGCAATAA TATTAGGAAT GTATGTCACA TACAAGAAAT GTCATGGAAA AAATGATGCT 
GAGTTTCTAG AGAAAACAGA CAATGATATG GACCCCATGC CATCATTCCA GGAGACAAAC 
AAGGGATTTC AACCAGATGA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered expression of the Olr59, Ethe1, and Slc10a2 genes in the liver of F344 rats by neonatal thyroid hormone disruption.
Journal of applied toxicology : JAT37(9)1030-1035(2017 Sep)
Matsubara K,Nakamura N,Sanoh S,Ohta S,Kitamura S,Uramaru N,Miyagawa S,Iguchi T,Fujimoto N

Diet1 functions in the FGF15/19 enterohepatic signaling axis to modulate bile acid and lipid levels.
Cell metabolism17(6)916-28(2013 Jun)
Vergnes L,Lee JM,Chin RG,Auwerx J,Reue K

Disruption of Stard10 gene alters the PPAR?-mediated bile acid homeostasis.
Biochimica et biophysica acta1831(2)459-68(2013 Feb)
Ito M,Yamanashi Y,Toyoda Y,Izumi-Nakaseko H,Oda S,Sugiyama A,Kuroda M,Suzuki H,Takada T,Adachi-Akahane S

Impact of lactobacilli on orally acquired listeriosis.
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)
Archambaud C,Nahori MA,Soubigou G,B?cavin C,Laval L,Lechat P,Smokvina T,Langella P,Lecuit M,Cossart P

Cytoprotective properties of rifampicin are related to the regulation of detoxification system and bile acid transporter expression during hepatocellular injury induced by hydrophobic bile acids.
Journal of hepato-biliary-pancreatic sciences18(5)740-50(2011 Sep)
Gonz?lez R,Cruz A,Ferr?n G,L?pez-Cillero P,Brice?o J,G?mez MA,Rufi?n S,Padillo J,De la Mata M,Marin JJ,Muntan? J