Ffar4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ffar4
Entrez Gene ID	294075
Full Name	free fatty acid receptor 4
Synonyms	Gpr120,O3far1
General protein information	Preferred Names free fatty acid receptor 4 Names free fatty acid receptor 4 G-protein coupled receptor 120 omega-3 fatty acid receptor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53

mRNA and Protein(s)

mRNA	Protein	Name
NM_001047088.1	NP_001040553.1	free fatty acid receptor 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	FFAR4	NP_859529.2
Gallus gallus (chicken)	O3FAR1	XP_003641529.2
Xenopus tropicalis (tropical clawed frog)	ffar4	XP_002937240.2
Rattus norvegicus (Norway rat)	Ffar4	NP_001040553.1
Pan troglodytes (chimpanzee)	O3FAR1	XP_001148225.1
Mus musculus (house mouse)	Ffar4	NP_861413.1
	LOC700014	XP_002808552.1
Canis lupus familiaris (dog)	O3FAR1	NP_001245268.1
Bos taurus (cattle)	LOC533266	XP_002698434.1

Gene Ontology
Bibliography

Related articles in PubMed

GPR120 suppresses adipose tissue lipolysis and synergizes with GPR40 in antidiabetic efficacy.
Satapati S, Qian Y, Wu MS, Petrov A, Dai G, Wang SP, Zhu Y, Shen X, Muise ES, Chen Y, Zycband E, Weinglass A, Di Salvo J, Debenham JS, Cox JM, Lan P, Shah V, Previs SF, Erion M, Kelley DE, Wang L, Howard AD, Shang J
Journal of lipid research58(8)1561-1578(2017 Aug)

Insulinotropic effects of GPR120 agonists are altered in obese diabetic and obese non-diabetic states.
Zhang D, So WY, Wang Y, Wu SY, Cheng Q, Leung PS
Clinical science (London, England : 1979)131(3)247-260(2017 Feb)

Arachidonic acid triggers [Ca2+]i increases in rat round spermatids by a likely GPR activation, ERK signalling and ER/acidic compartments Ca2+ release.
Paillamanque J, Sanchez-Tusie A, Carmona EM, Trevi?o CL, Sandoval C, Nualart F, Osses N, Reyes JG
PloS one12(2)e0172128(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GPR120 expression in beta-cells and GPR120-mediated insulinotropic effects are altered in obesity and diabetic states in distinct ways, and these alterations may be mediated by PPARgamma.
Title: Insulinotropic effects of GPR120 agonists are altered in obese diabetic and obese non-diabetic states.

Data show that G protein-coupled receptor 120 protein (GPR120) appears strongly on interstitial cells and also on spermatogenic cells.
Title: Arachidonic acid triggers [Ca2+]i increases in rat round spermatids by a likely GPR activation, ERK signalling and ER/acidic compartments Ca2+ release.

demonstrated a GPR120-mediated novel anti-inflammatory pathway in specific intestinal epithelial cell types that could be of therapeutic relevance to intestinal inflammatory disorders
Title: A novel anti-inflammatory role of GPR120 in intestinal epithelial cells.

results indicated that the beneficial metabolic role of DHA was attributed to its ability to regulate glucose via the GPR120-mediated AMPK pathway in the skeletal muscles
Title: Endogenous Ligand for GPR120, Docosahexaenoic Acid, Exerts Benign Metabolic Effects on the Skeletal Muscles via AMP-activated Protein Kinase Pathway.

opposite effects of GPR120 and GPR40 may be involved in the cell motile activity stimulated by ethionine in WB-F344 cells
Title: Opposite effects of GPR120 and GPR40 on cell motile activity induced by ethionine in liver epithelial cells.

The following Ffar4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ffar4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09811	NM_001047088.1 Latest version!	Rattus norvegicus free fatty acid receptor 4 (Ffar4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ffar4 ( NM_001047088.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001040553.1 Rattus norvegicus free fatty acid receptor 4 (Ffar4), mRNA.

Price & Availability↑

CloneID	ORa09811
Clone ID Related Accession (Same CDS sequence)	NM_001047088.1
Accession Version	NM_001047088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	free fatty acid receptor 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB207868.1. On or before Sep 7, 2006 this sequence version replaced XM_215281.4, XM_001080642.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB207868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCCTG AGTGTGCGCA GACGACGGGC CCTGGCCCCT CGCGCACCCC GGACCAAGTC 
AATCGCACCC ACTTCCCTTT CTTCTCGGAT GTCAAGGGCG ACCACCGGCT GGTGCTGAGC 
GTCCTGGAGA CCACCGTTCT GGGACTCATC TTTGTGGTCT CACTGCTGGG CAACGTGTGT 
GCCCTGGTGC TGGTGGTGCG CCGTCGGCGC CGTGGGGCGA CAGTCAGCTT GGTGCTCAAC 
CTCTTCTGCG CGGATTTGCT CTTCACCAGC GCCATCCCTC TAGTGCTCGT GGTGCGCTGG 
ACTGAAGCCT GGCTGCTGGG GCCCGTCGTC TGCCACCTGC TCTTCTACGT GATGACCATG 
AGCGGCAGCG TCACGATCCT CACGCTGGCC GCGGTCAGCC TGGAGCGCAT GGTGTGCATC 
GTGCGCCTGC GGCGCGGCTT GAGCGGCCCG GGGCGGCGGA CTCAGGCGGC GCTGCTGGCT 
TTCATATGGG GTTACTCGGC GCTCGCCGCG CTGCCCCTCT GCATCTTGTT CCGCGTGGTC 
CCGCAGCGCC TTCCCGGCGG GGACCAGGAA ATTCCGATCT GCACATTGGA TTGGCCCAAC 
CGCATAGGAG AAATCTCATG GGATGTGTTT TTTGTGACTT TGAACTTCCT GGTACCAGGA 
CTGGTCATTG TGATCAGCTA CTCCAAGATT TTACAGATCA CGAAAGCCTC GCGGAAGAGG 
CTTACGCTGA GCTTGGCATA CTCCGAGAGC CACCAGATCC GAGTGTCCCA GCAGGACTAC 
CGGCTCTTCC GAACGCTCTT CCTGCTCATG GTTTCCTTCT TCATCATGTG GAGTCCCATC 
ATCATCACCA TCCTCCTCAT CTTGATCCAG AACTTCCGGC AGGACCTGGT TATCTGGCCG 
TCCCTTTTCT TCTGGGTGGT GGCCTTCACG TTTGCCAACT CCGCCCTAAA CCCCATTCTG 
TACAACATGT CGCTGTTCAG GAGCGAGTGG AGGAAGATTT TTTGCTGCTT CTTTTTCCCA 
GAGAAGGGAG CCATTTTTAC AGAAACGTCT ATCAGGCGAA ATGACTTGTC TGTTATTTCC 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001040553.1
CDS	46..1131
Translation	MSPECAQTTGPGPSRTPDQVNRTHFPFFSDVKGDHRLVLSVLETTVLGLIFVVSLLGNVCALVLVVRRRRRGATVSLVLNLFCADLLFTSAIPLVLVVRWTEAWLLGPVVCHLLFYVMTMSGSVTILTLAAVSLERMVCIVRLRRGLSGPGRRTQAALLAFIWGYSALAALPLCILFRVVPQRLPGGDQEIPICTLDWPNRIGEISWDVFFVTLNFLVPGLVIVISYSKILQITKASRKRLTLSLAYSESHQIRVSQQDYRLFRTLFLLMVSFFIMWSPIIITILLILIQNFRQDLVIWPSLFFWVVAFTFANSALNPILYNMSLFRSEWRKIFCCFFFPEKGAIFTETSIRRNDLSVIST

Target ORF information:

RefSeq Version	NM_001047088.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus free fatty acid receptor 4 (Ffar4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047088.1

ORF Insert Sequence:

ATGTCCCCTG AGTGTGCGCA GACGACGGGC CCTGGCCCCT CGCGCACCCC GGACCAAGTC 
AATCGCACCC ACTTCCCTTT CTTCTCGGAT GTCAAGGGCG ACCACCGGCT GGTGCTGAGC 
GTCCTGGAGA CCACCGTTCT GGGACTCATC TTTGTGGTCT CACTGCTGGG CAACGTGTGT 
GCCCTGGTGC TGGTGGTGCG CCGTCGGCGC CGTGGGGCGA CAGTCAGCTT GGTGCTCAAC 
CTCTTCTGCG CGGATTTGCT CTTCACCAGC GCCATCCCTC TAGTGCTCGT GGTGCGCTGG 
ACTGAAGCCT GGCTGCTGGG GCCCGTCGTC TGCCACCTGC TCTTCTACGT GATGACCATG 
AGCGGCAGCG TCACGATCCT CACGCTGGCC GCGGTCAGCC TGGAGCGCAT GGTGTGCATC 
GTGCGCCTGC GGCGCGGCTT GAGCGGCCCG GGGCGGCGGA CTCAGGCGGC GCTGCTGGCT 
TTCATATGGG GTTACTCGGC GCTCGCCGCG CTGCCCCTCT GCATCTTGTT CCGCGTGGTC 
CCGCAGCGCC TTCCCGGCGG GGACCAGGAA ATTCCGATCT GCACATTGGA TTGGCCCAAC 
CGCATAGGAG AAATCTCATG GGATGTGTTT TTTGTGACTT TGAACTTCCT GGTACCAGGA 
CTGGTCATTG TGATCAGCTA CTCCAAGATT TTACAGATCA CGAAAGCCTC GCGGAAGAGG 
CTTACGCTGA GCTTGGCATA CTCCGAGAGC CACCAGATCC GAGTGTCCCA GCAGGACTAC 
CGGCTCTTCC GAACGCTCTT CCTGCTCATG GTTTCCTTCT TCATCATGTG GAGTCCCATC 
ATCATCACCA TCCTCCTCAT CTTGATCCAG AACTTCCGGC AGGACCTGGT TATCTGGCCG 
TCCCTTTTCT TCTGGGTGGT GGCCTTCACG TTTGCCAACT CCGCCCTAAA CCCCATTCTG 
TACAACATGT CGCTGTTCAG GAGCGAGTGG AGGAAGATTT TTTGCTGCTT CTTTTTCCCA 
GAGAAGGGAG CCATTTTTAC AGAAACGTCT ATCAGGCGAA ATGACTTGTC TGTTATTTCC 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GPR120 suppresses adipose tissue lipolysis and synergizes with GPR40 in antidiabetic efficacy.
Journal of lipid research58(8)1561-1578(2017 Aug)
Satapati S,Qian Y,Wu MS,Petrov A,Dai G,Wang SP,Zhu Y,Shen X,Muise ES,Chen Y,Zycband E,Weinglass A,Di Salvo J,Debenham JS,Cox JM,Lan P,Shah V,Previs SF,Erion M,Kelley DE,Wang L,Howard AD,Shang J

Insulinotropic effects of GPR120 agonists are altered in obese diabetic and obese non-diabetic states.
Clinical science (London, England : 1979)131(3)247-260(2017 Feb)
Zhang D,So WY,Wang Y,Wu SY,Cheng Q,Leung PS

Arachidonic acid triggers [Ca2+]i increases in rat round spermatids by a likely GPR activation, ERK signalling and ER/acidic compartments Ca2+ release.
PloS one12(2)e0172128(2017)
Paillamanque J,Sanchez-Tusie A,Carmona EM,Trevi?o CL,Sandoval C,Nualart F,Osses N,Reyes JG