Sfr1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sfr1
Entrez Gene ID	294030
Full Name	SWI5-dependent homologous recombination repair protein 1
Synonyms	LRRGT00030,Meir5,RGD1305481
General protein information	Preferred Names SWI5-dependent homologous recombination repair protein 1 Names swi5-dependent recombination DNA repair protein 1 homolog MEI5 meiotic recombination protein homolog MEI5 recombination repair protein homolog SWI5-dependent recombination repair 1 liver regeneration-related protein LRRGT00030 meiosis protein 5 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589044.1	XP_017444533.1	swi5-dependent recombination DNA repair protein 1 homolog isoform X1
NM_001047856.2	NP_001041321.2	swi5-dependent recombination DNA repair protein 1 homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	SFR1	NP_001002759.1
Pan troglodytes (chimpanzee)	SFR1	XP_003312845.1
	LOC715145	XP_001114219.1
Xenopus tropicalis (tropical clawed frog)	sfr1	XP_004915900.1
Canis lupus familiaris (dog)	SFR1	XP_005637751.1
Mus musculus (house mouse)	Sfr1	NP_080653.2
Gallus gallus (chicken)	SFR1	XP_001234168.2
Bos taurus (cattle)	SFR1	XP_003587924.1
Rattus norvegicus (Norway rat)	Sfr1	NP_001041321.2
Danio rerio (zebrafish)	sfr1	NP_001076329.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA homologous recombination factor SFR1 physically and functionally interacts with estrogen receptor alpha.
Feng Y, Singleton D, Guo C, Gardner A, Pakala S, Kumar R, Jensen E, Zhang J, Khan S
PloS one8(7)e68075(2013)

The role of the human SWI5-MEI5 complex in homologous recombination repair.
Yuan J, Chen J
The Journal of biological chemistry286(11)9888-93(2011 Mar)

Role for the mammalian Swi5-Sfr1 complex in DNA strand break repair through homologous recombination.
Akamatsu Y, Jasin M
PLoS genetics6(10)e1001160(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sfr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sfr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04196	NM_001047856.2 Latest version!	Rattus norvegicus SWI5-dependent homologous recombination repair protein 1 (Sfr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa56532	XM_017589044.1 Latest version!	Rattus norvegicus SWI5-dependent homologous recombination repair protein 1 (Sfr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sfr1 ( NM_001047856.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001041321.2 Rattus norvegicus SWI5-dependent homologous recombination repair protein 1 (Sfr1), mRNA.

Price & Availability↑

CloneID	ORa04196
Clone ID Related Accession (Same CDS sequence)	NM_001047856.2
Accession Version	NM_001047856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-21
Organism	Rattus norvegicus(Norway rat)
Product	swi5-dependent recombination DNA repair protein 1 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM118556.1 and AC096311.8. On Mar 4, 2011 this sequence version replaced NM_001047856.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FM118556.1, FQ214391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGAGG AAGAAGGAAA TCAGGAGATC ACTTCCAAGA TGGAAAACCC CTCAGATTCA 
GCATCCACTT CACCTGACAT TCCTCAGACC AGTGAGAATC CACCATCACC CCCAACCTCA 
CCTGACAAGC CTCAGACCAG TGAAAATCCA CCATCACTCC ATTCGAATAG TTCAGGAAAA 
CAACCTATGA GTGGGACACT TAAAGAAAGA TTAAAGAAAG CAAGAGCTTC ATCTCAACCT 
TTTTGTAGTG TGGTGAAGCG TATTAAGGTA GAGAATGAAG AAAATGATCA GACCCTTTCA 
GAACCAGGAG AATCTTCAAA AGAAGAAAAC TGTTCAAAAG CCCAAGAAAG CCTGGAAAAC 
AAAGACAATG AACCTGAAAA AGAGAGCTCA GAGGATAAAA ATACGAGTGA ATCTAAGTCA 
CTTGATACTG GGTCATCCAG TGTCCTCCAA AAAGATTCTA CTGAGAAGAC TATAAAGCAA 
ACTTTGAAGG AAGAAAAAGC AAAACTGACT AGGCAGGTTC AAGAGAAGGA AGACCTTCTT 
CGGAGGTTAA AGTTAGTCAA AATGTACAGA ATAAAGAATG ACGTGACTGA GTTAGAGGAT 
TTAATAAAGA AGTGGAGGAG GTGTGGCCAG CGGCTGCTGT GCGAGTTGCA GTCAATCATG 
TCCGAGGATG AGGACGAGAA GCTGACCCTC ACTGAGTTGA TCGACTACTA TGGGATAGAC 
GACAAATTGC TGCACTATAA TAGAACTGAA GAAGAGTTCA CAGGGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041321.2
CDS	65..835
Translation	MAEEEGNQEITSKMENPSDSASTSPDIPQTSENPPSPPTSPDKPQTSENPPSLHSNSSGKQPMSGTLKERLKKARASSQPFCSVVKRIKVENEENDQTLSEPGESSKEENCSKAQESLENKDNEPEKESSEDKNTSESKSLDTGSSSVLQKDSTEKTIKQTLKEEKAKLTRQVQEKEDLLRRLKLVKMYRIKNDVTELEDLIKKWRRCGQRLLCELQSIMSEDEDEKLTLTELIDYYGIDDKLLHYNRTEEEFTGV

Target ORF information:

RefSeq Version	NM_001047856.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SWI5-dependent homologous recombination repair protein 1 (Sfr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047856.2

ORF Insert Sequence:

ATGGCTGAGG AAGAAGGAAA TCAGGAGATC ACTTCCAAGA TGGAAAACCC CTCAGATTCA 
GCATCCACTT CACCTGACAT TCCTCAGACC AGTGAGAATC CACCATCACC CCCAACCTCA 
CCTGACAAGC CTCAGACCAG TGAAAATCCA CCATCACTCC ATTCGAATAG TTCAGGAAAA 
CAACCTATGA GTGGGACACT TAAAGAAAGA TTAAAGAAAG CAAGAGCTTC ATCTCAACCT 
TTTTGTAGTG TGGTGAAGCG TATTAAGGTA GAGAATGAAG AAAATGATCA GACCCTTTCA 
GAACCAGGAG AATCTTCAAA AGAAGAAAAC TGTTCAAAAG CCCAAGAAAG CCTGGAAAAC 
AAAGACAATG AACCTGAAAA AGAGAGCTCA GAGGATAAAA ATACGAGTGA ATCTAAGTCA 
CTTGATACTG GGTCATCCAG TGTCCTCCAA AAAGATTCTA CTGAGAAGAC TATAAAGCAA 
ACTTTGAAGG AAGAAAAAGC AAAACTGACT AGGCAGGTTC AAGAGAAGGA AGACCTTCTT 
CGGAGGTTAA AGTTAGTCAA AATGTACAGA ATAAAGAATG ACGTGACTGA GTTAGAGGAT 
TTAATAAAGA AGTGGAGGAG GTGTGGCCAG CGGCTGCTGT GCGAGTTGCA GTCAATCATG 
TCCGAGGATG AGGACGAGAA GCTGACCCTC ACTGAGTTGA TCGACTACTA TGGGATAGAC 
GACAAATTGC TGCACTATAA TAGAACTGAA GAAGAGTTCA CAGGGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfr1 ( XM_017589044.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444533.1 Rattus norvegicus SWI5-dependent homologous recombination repair protein 1 (Sfr1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56532
Clone ID Related Accession (Same CDS sequence)	XM_017589044.1
Accession Version	XM_017589044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	swi5-dependent recombination DNA repair protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACC CCTCAGATTC AGCATCCACT TCACCTGACA TTCCTCAGAC CAGTGAGAAT 
CCACCATCAC CCCCAACCTC ACCTGACAAG CCTCAGACCA GTGAAAATCC ACCATCACTC 
CATTCGAATA GTTCAGGAAA ACAACCTATG AGTGGGACAC TTAAAGAAAG ATTAAAGAAA 
GCAAGAGCTT CATCTCAACC TTTTTGTAGT GTGGTGAAGC GTATTAAGGT AGAGAATGAA 
GAAAATGATC AGACCCTTTC AGAACCAGGA GAATCTTCAA AAGAAGAAAA CTGTTCAAAA 
GCCCAAGAAA GCCTGGAAAA CAAAGACAAT GAACCTGAAA AAGAGAGCTC AGAGGATAAA 
AATACGAGTG AATCTAAGTC ACTTGATACT GGGTCATCCA GTGTCCTCCA AAAAGATTCT 
ACTGAGAAGA CTATAAAGCA AACTTTGAAG GAAGAAAAAG CAAAACTGAC TAGGCAGGTT 
CAAGAGAAGG AAGACCTTCT TCGGAGGTTA AAGTTAGTCA AAATGTACAG AATAAAGAAT 
GACGTGACTG AGTTAGAGGA TTTAATAAAG AAGTGGAGGA GGTGTGGCCA GCGGCTGCTG 
TGCGAGTTGC AGTCAATCAT GTCCGAGGAT GAGGACGAGA AGCTGACCCT CACTGAGTTG 
ATCGACTACT ATGGGATAGA CGACAAATTG CTGCACTATA ATAGAACTGA AGAAGAGTTC 
ACAGGGGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444533.1
CDS	51..782
Translation	MENPSDSASTSPDIPQTSENPPSPPTSPDKPQTSENPPSLHSNSSGKQPMSGTLKERLKKARASSQPFCSVVKRIKVENEENDQTLSEPGESSKEENCSKAQESLENKDNEPEKESSEDKNTSESKSLDTGSSSVLQKDSTEKTIKQTLKEEKAKLTRQVQEKEDLLRRLKLVKMYRIKNDVTELEDLIKKWRRCGQRLLCELQSIMSEDEDEKLTLTELIDYYGIDDKLLHYNRTEEEFTGV

Target ORF information:

RefSeq Version	XM_017589044.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SWI5-dependent homologous recombination repair protein 1 (Sfr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589044.1

ORF Insert Sequence:

ATGGAAAACC CCTCAGATTC AGCATCCACT TCACCTGACA TTCCTCAGAC CAGTGAGAAT 
CCACCATCAC CCCCAACCTC ACCTGACAAG CCTCAGACCA GTGAAAATCC ACCATCACTC 
CATTCGAATA GTTCAGGAAA ACAACCTATG AGTGGGACAC TTAAAGAAAG ATTAAAGAAA 
GCAAGAGCTT CATCTCAACC TTTTTGTAGT GTGGTGAAGC GTATTAAGGT AGAGAATGAA 
GAAAATGATC AGACCCTTTC AGAACCAGGA GAATCTTCAA AAGAAGAAAA CTGTTCAAAA 
GCCCAAGAAA GCCTGGAAAA CAAAGACAAT GAACCTGAAA AAGAGAGCTC AGAGGATAAA 
AATACGAGTG AATCTAAGTC ACTTGATACT GGGTCATCCA GTGTCCTCCA AAAAGATTCT 
ACTGAGAAGA CTATAAAGCA AACTTTGAAG GAAGAAAAAG CAAAACTGAC TAGGCAGGTT 
CAAGAGAAGG AAGACCTTCT TCGGAGGTTA AAGTTAGTCA AAATGTACAG AATAAAGAAT 
GACGTGACTG AGTTAGAGGA TTTAATAAAG AAGTGGAGGA GGTGTGGCCA GCGGCTGCTG 
TGCGAGTTGC AGTCAATCAT GTCCGAGGAT GAGGACGAGA AGCTGACCCT CACTGAGTTG 
ATCGACTACT ATGGGATAGA CGACAAATTG CTGCACTATA ATAGAACTGA AGAAGAGTTC 
ACAGGGGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA homologous recombination factor SFR1 physically and functionally interacts with estrogen receptor alpha.
PloS one8(7)e68075(2013)
Feng Y,Singleton D,Guo C,Gardner A,Pakala S,Kumar R,Jensen E,Zhang J,Khan S

The role of the human SWI5-MEI5 complex in homologous recombination repair.
The Journal of biological chemistry286(11)9888-93(2011 Mar)
Yuan J,Chen J

Role for the mammalian Swi5-Sfr1 complex in DNA strand break repair through homologous recombination.
PLoS genetics6(10)e1001160(2010 Oct)
Akamatsu Y,Jasin M