B3gat3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	B3gat3
Entrez Gene ID	293722
Full Name	beta-1,3-glucuronyltransferase 3
General protein information	Preferred Names beta-1,3-glucuronyltransferase 3 Names galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128184.1	NP_001121656.1	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	B3GAT3	NP_001229555.1
Canis lupus familiaris (dog)	B3GAT3	XP_540906.2
Bos taurus (cattle)	B3GAT3	NP_991374.2
Homo sapiens (human)	B3GAT3	NP_036332.2
Mus musculus (house mouse)	B3gat3	NP_077218.1
Rattus norvegicus (Norway rat)	B3gat3	NP_001121656.1
Danio rerio (zebrafish)	b3gat3	NP_001015059.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002801	XP_312113.5
Drosophila melanogaster (fruit fly)	GlcAT-I	NP_726910.1
Caenorhabditis elegans (roundworm)	sqv-8	NP_496076.1
Xenopus tropicalis (tropical clawed frog)	b3gat3	NP_001025520.1

Gene Ontology
Bibliography

Related articles in PubMed

Skeletal dysplasia in a consanguineous clan from the island of Nias/Indonesia is caused by a novel mutation in B3GAT3.
Budde BS, Mizumoto S, Kogawa R, Becker C, Altm?ller J, Thiele H, R?schendorf F, Toliat MR, Kaleschke G, H?mmerle JM, H?hne W, Sugahara K, N?rnberg P, Kennerknecht I
Human genetics134(7)691-704(2015 Jul)

Identification of phosphatase that dephosphorylates xylose in the glycosaminoglycan-protein linkage region of proteoglycans.
Koike T, Izumikawa T, Sato B, Kitagawa H
The Journal of biological chemistry289(10)6695-708(2014 Mar)

Faulty initiation of proteoglycan synthesis causes cardiac and joint defects.
Baasanjav S, Al-Gazali L, Hashiguchi T, Mizumoto S, Fischer B, Horn D, Seelow D, Ali BR, Aziz SA, Langer R, Saleh AA, Becker C, N?rnberg G, Cantagrel V, Gleeson JG, Gomez D, Michel JB, Stricker S, Lindner TH, N?rnberg P, Sugahara K, Mundlos S, Hoffmann K
American journal of human genetics89(1)15-27(2011 Jul)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GlcAT-I has a role in controlling and reversing articular cartilage defects
Title: Stimulation of proteoglycan synthesis by glucuronosyltransferase-I gene delivery: a strategy to promote cartilage repair.

The following B3gat3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B3gat3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02182	NM_001128184.1 Latest version!	Rattus norvegicus beta-1,3-glucuronyltransferase 3 (B3gat3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B3gat3 ( NM_001128184.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001121656.1 Rattus norvegicus beta-1,3-glucuronyltransferase 3 (B3gat3), mRNA.

Price & Availability↑

CloneID	ORa02182
Clone ID Related Accession (Same CDS sequence)	NM_001128184.1
Accession Version	NM_001128184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473953.1. ##Evidence-Data-START## Transcript exon combination :: BC168710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTGA AGCTGAAAAA CGTGTTTCTT GCCTACTTCC TGGTGTCGAT CGCCGGCCTC 
CTCTACGCTC TGGTGCAGCT CGGCCAACCT TGTGACTGCC TCCCTCCACT TCGAGCTGCA 
GCTGAGCAGC TCCGGCAAAA GGACCTGAGG ATATCCCAGC TGCAAGCCGA CCTCCGTCGC 
CCACCCCCTG CCCCAGCCCA GCCCCCAGAA CCTGAGGCCC TGCCTACTAT CTATGTCATT 
ACCCCCACCT ATGCCAGGCT AGTACAGAAG GCAGAGCTGG TTCGGCTGTC CCAGACCCTG 
AGCTTGGTGC CCCGTCTACA CTGGCTGCTG GTGGAGGATG CTGAGAGCCC TACCCCGCTG 
GTCTCGGGGC TGTTGGCTGC CTCTGGTCTC CTCTTCACAC ACCTGGCTGT CCTTACCCCC 
AAGGCTCAGC GGCTTAGGGA AGGTGAGCCA GGCTGGGTCC GGCCCCGAGG AGTAGAACAG 
CGCAACAAAG CCCTGGACTG GCTCAGAGGA AAAGGGGGTG CTGTAGGGGG AGAGAAGGAC 
TCACCACCAC CAGGCACCCA AGGAGTCGTG TATTTTGCTG ACGATGATAA CACCTACAGC 
CGGGAGCTCT TTAAGGAGAT GCGTTGGACT CGTGGTGTCT CAGTGTGGCC TGTGGGGCTG 
GTGGGCGGCC TGCGATTTGA GGGCCCTCGG GTACAGGATG GCCGTGTTGT GGGTTTCCAC 
ACAGCATGGG AACCCAACAG GCCCTTTCCC CTGGACATGG CAGGATTTGC AGTTTCCCTG 
CCCTTGCTGC TGGCTAAGCC TAATGCCCAG TTTGATGCTA CTGCACCCCG GGGCCACCTG 
GAAAGTAGTC TCCTGAGCCA TCTTGTAGAT CCCAAGGACC TGGAGCCCCG GGCTGCCAAC 
TGTACTCAGG TACTGGTGTG GCATACCCGG ACAGAGAAAC CGAAGATGAA GCAGGAGGAA 
CAGCTACAGC GACAGGGCCA GGGGTCAGAC CCAGCTATTG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121656.1
CDS	179..1186
Translation	MKLKLKNVFLAYFLVSIAGLLYALVQLGQPCDCLPPLRAAAEQLRQKDLRISQLQADLRRPPPAPAQPPEPEALPTIYVITPTYARLVQKAELVRLSQTLSLVPRLHWLLVEDAESPTPLVSGLLAASGLLFTHLAVLTPKAQRLREGEPGWVRPRGVEQRNKALDWLRGKGGAVGGEKDSPPPGTQGVVYFADDDNTYSRELFKEMRWTRGVSVWPVGLVGGLRFEGPRVQDGRVVGFHTAWEPNRPFPLDMAGFAVSLPLLLAKPNAQFDATAPRGHLESSLLSHLVDPKDLEPRAANCTQVLVWHTRTEKPKMKQEEQLQRQGQGSDPAIEV

Target ORF information:

RefSeq Version	NM_001128184.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,3-glucuronyltransferase 3 (B3gat3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128184.1

ORF Insert Sequence:

ATGAAGCTGA AGCTGAAAAA CGTGTTTCTT GCCTACTTCC TGGTGTCGAT CGCCGGCCTC 
CTCTACGCTC TGGTGCAGCT CGGCCAACCT TGTGACTGCC TCCCTCCACT TCGAGCTGCA 
GCTGAGCAGC TCCGGCAAAA GGACCTGAGG ATATCCCAGC TGCAAGCCGA CCTCCGTCGC 
CCACCCCCTG CCCCAGCCCA GCCCCCAGAA CCTGAGGCCC TGCCTACTAT CTATGTCATT 
ACCCCCACCT ATGCCAGGCT AGTACAGAAG GCAGAGCTGG TTCGGCTGTC CCAGACCCTG 
AGCTTGGTGC CCCGTCTACA CTGGCTGCTG GTGGAGGATG CTGAGAGCCC TACCCCGCTG 
GTCTCGGGGC TGTTGGCTGC CTCTGGTCTC CTCTTCACAC ACCTGGCTGT CCTTACCCCC 
AAGGCTCAGC GGCTTAGGGA AGGTGAGCCA GGCTGGGTCC GGCCCCGAGG AGTAGAACAG 
CGCAACAAAG CCCTGGACTG GCTCAGAGGA AAAGGGGGTG CTGTAGGGGG AGAGAAGGAC 
TCACCACCAC CAGGCACCCA AGGAGTCGTG TATTTTGCTG ACGATGATAA CACCTACAGC 
CGGGAGCTCT TTAAGGAGAT GCGTTGGACT CGTGGTGTCT CAGTGTGGCC TGTGGGGCTG 
GTGGGCGGCC TGCGATTTGA GGGCCCTCGG GTACAGGATG GCCGTGTTGT GGGTTTCCAC 
ACAGCATGGG AACCCAACAG GCCCTTTCCC CTGGACATGG CAGGATTTGC AGTTTCCCTG 
CCCTTGCTGC TGGCTAAGCC TAATGCCCAG TTTGATGCTA CTGCACCCCG GGGCCACCTG 
GAAAGTAGTC TCCTGAGCCA TCTTGTAGAT CCCAAGGACC TGGAGCCCCG GGCTGCCAAC 
TGTACTCAGG TACTGGTGTG GCATACCCGG ACAGAGAAAC CGAAGATGAA GCAGGAGGAA 
CAGCTACAGC GACAGGGCCA GGGGTCAGAC CCAGCTATTG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Skeletal dysplasia in a consanguineous clan from the island of Nias/Indonesia is caused by a novel mutation in B3GAT3.
Human genetics134(7)691-704(2015 Jul)
Budde BS,Mizumoto S,Kogawa R,Becker C,Altm?ller J,Thiele H,R?schendorf F,Toliat MR,Kaleschke G,H?mmerle JM,H?hne W,Sugahara K,N?rnberg P,Kennerknecht I

Identification of phosphatase that dephosphorylates xylose in the glycosaminoglycan-protein linkage region of proteoglycans.
The Journal of biological chemistry289(10)6695-708(2014 Mar)
Koike T,Izumikawa T,Sato B,Kitagawa H

Faulty initiation of proteoglycan synthesis causes cardiac and joint defects.
American journal of human genetics89(1)15-27(2011 Jul)
Baasanjav S,Al-Gazali L,Hashiguchi T,Mizumoto S,Fischer B,Horn D,Seelow D,Ali BR,Aziz SA,Langer R,Saleh AA,Becker C,N?rnberg G,Cantagrel V,Gleeson JG,Gomez D,Michel JB,Stricker S,Lindner TH,N?rnberg P,Sugahara K,Mundlos S,Hoffmann K

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR