Sptbn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptbn2
Entrez Gene ID	29211
Full Name	spectrin, beta, non-erythrocytic 2
Synonyms	Spnb3
General protein information	Preferred Names spectrin, beta, non-erythrocytic 2 Names spectrin beta chain, non-erythrocytic 2 beta SpIII sigma 1 beta-III spectrin beta-spectrin 3 glutamate transporter EAAT4-associated protein 41 spectrin beta chain, brain 2 spectrin, non-erythroid beta chain 2 spectrin-like protein GTRAP41
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	may modulate the structural integrity of the Golgi apparatus; binds preferentially to Arp1 rather than actin to mediate the direct interaction of dynein and dynactin with vesicles [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019167.2	NP_062040.2	spectrin beta chain, non-erythrocytic 2
XM_008760092.2	XP_008758314.1	spectrin beta chain, non-erythrocytic 2 isoform X1
XM_008760094.2	XP_008758316.1	spectrin beta chain, non-erythrocytic 2 isoform X2
XM_017588856.1	XP_017444345.1	spectrin beta chain, non-erythrocytic 2 isoform X2
XM_008760093.2	XP_008758315.1	spectrin beta chain, non-erythrocytic 2 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPTBN2	NP_008877.1
	SPTBN2	XP_001108262.2
Mus musculus (house mouse)	Sptbn2	NP_067262.1
Xenopus tropicalis (tropical clawed frog)	LOC100486199	XP_004913752.1
Pan troglodytes (chimpanzee)	SPTBN2	XP_001172486.2
Danio rerio (zebrafish)	LOC558044	NP_001093459.1
Rattus norvegicus (Norway rat)	Sptbn2	NP_062040.1
Canis lupus familiaris (dog)	SPTBN2	XP_540827.3

Gene Ontology
Bibliography

Related articles in PubMed

?III Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons.
Efimova N, Korobova F, Stankewich MC, Moberly AH, Stolz DB, Wang J, Kashina A, Ma M, Svitkina T
The Journal of neuroscience : the official journal of the Society for Neuroscience37(27)6442-6459(2017 Jul)

Degradation of ?II-Spectrin Protein by Calpain-2 and Caspase-3 Under Neurotoxic and Traumatic Brain Injury Conditions.
Kobeissy FH, Liu MC, Yang Z, Zhang Z, Zheng W, Glushakova O, Mondello S, Anagli J, Hayes RL, Wang KK
Molecular neurobiology52(1)696-709(2015 Aug)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

?III spectrin regulates the structural integrity and the secretory protein transport of the Golgi complex.
Salcedo-Sicilia L, Granell S, Jovic M, Sicart A, Mato E, Johannes L, Balla T, Egea G
The Journal of biological chemistry288(4)2157-66(2013 Jan)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results revealed that betaII-spectrin was degraded into major fragments of 110/85 kDa by calpain-2 activation and 108/80 kDa by caspase-3 activation.
Title: Degradation of ?II-Spectrin Protein by Calpain-2 and Caspase-3 Under Neurotoxic and Traumatic Brain Injury Conditions.

betaIII spectrin regulates the structural integrity and the secretory protein transport of the Golgi complex
Title: ?III spectrin regulates the structural integrity and the secretory protein transport of the Golgi complex.

the embryonic mammalian heart expresses a significant amount of alpha II-spectrin with a reduced avidity for beta-spectrin and the ability to promote myocyte growth
Title: Characterization and expression of a heart-selective alternatively spliced variant of alpha II-spectrin, cardi+, during development in the rat.

role in fragmentation of the Golgi apparatus
Title: Fragmentation of the Golgi apparatus. A role for beta III spectrin and synthesis of phosphatidylinositol 4,5-bisphosphate.

Arp1 binds directly to the Golgi-associated betaIII spectrin isoform; interaction recruits dynein and dynactin to intracellular membranes and provides a direct link between the microtubule motor complex and its membrane-bounded cargo
Title: beta III spectrin binds to the Arp1 subunit of dynactin.

The following Sptbn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptbn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa23710	NM_019167.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa23710	XM_008760094.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa23710	XM_017588856.1 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa43234	XM_008760092.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa43235	XM_008760093.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptbn2 ( NM_019167.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062040.2 Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), mRNA.

Price & Availability↑

CloneID	ORa23710
Clone ID Related Accession (Same CDS sequence)	NM_019167.2 , XM_008760094.2 , XM_017588856.1
Accession Version	NM_019167.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119332.6. On Sep 2, 2015 this sequence version replaced NM_019167.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB001347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAGCA CCCTGTCACC CACTGACTTC GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCCGAC TCAGATTGGG ACAATGACAG CAGTTCAGCC 
CGCCTCTTTG AGAGGTCCAG AATTAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCCAC CAAGGGCCGG ATGCGGATTC ACTGCCTGGA GAATGTCGAC 
AAAGCACTGC AGTTCCTGAA GGAGCAGAAG GTGCACCTGG AAAACATGGG CTCCCACGAC 
ATTGTGGATG GGAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGGTATC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAACGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAC 
GTAGACCAAC CCGATGAGAA GTCCATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCGAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGGATTG GCAAGGTCCT GGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAT GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATCGAGCAAA CGATTGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGCGTC 
CAGAACCAGC TGCAGTCTTT CAATTCCTAC CGCACGGTGG AGAAGCCACC CAAGTTCACA 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GTAAGCTGCG GGCCAACAAC 
CAGAAAGTCT ACACACCACG CGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTAGAGA AAGCCGAACA TGAGCGAGAG CTGGCCCTGC GCACGGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGCCT CGTCTCCCAG GACAACTTTG GCCTGGAGCT GGCAGCAGTG 
GAGGCAGCAG TGCGGAAGCA TGAAGCCATT GAGACAGACA TTGTGGCCTA CAGCGGCCGG 
GTGCAAGCGG TGGACGCCGT AGCCGCAGAA CTGGCCGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CGCGGCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG AGAGATGGTG 
GCCGCCCGCC GTGAGCGGCT CCTTCTCAAC CTGGAGCTGC AGAAGGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTAGGC 
AAGCATCTGG CTGGAGTGGA AGATCTACTG CAACTACACG AACTGGTGGA GGCGGACATT 
GCAGTTCAGG CTGAGAGGGT GCGAGCGGTC AGCGCCTCTG CCCTGCGCTT CTGCGACCCC 
GGGAAAGAGT ATAGACCATG TGACCCACAG CTAGTGTCAG AGAGGGTAGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG CGAATTGGCA GCAACTCGAA GGGCCCGACT GGAAGAGTCC 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCCG AGGCCTGGGT TCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGAGACAGGC CGGGACCTGA CTGGTGTCCT CCGCCTGCTC 
AATAAGCACA CAGCCCTACG GGGTGAGATG AGTGGCCGCC TGGGGCCCCT GAAGCTCACC 
CTGGAACAAG GTCAGCAGTT AGTTGCCGAG GGCCACCCTG GAGCTAACCA AGCCTCAACC 
CGTGCAGCAG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCGGCTAG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCCAA TGACATGGAG 
GCTTGGTTGG TGGACGCACT ACGCCTGGTA TCTAGCCCTG AGGTAGGGCA CGATGAGTTC 
TCCACGCAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAGG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCTTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCTGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGG 
GCAGGTGAGC GTGCACGAGC CCTGGAAGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCCGGGGCTT GTGGGCTCTG GGTAGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAACAGAGGT TTGAGACCTT AGAGCCCGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAGTGACA TAGCTGAGCA GTTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTCA GGTCCCTGGC AGATGGCAAA AAGGCAGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAAAA GACCAAGGTC 
ATTGAGTCTA CCCAGGACCT AGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
CTGGCAGGCA CTGAGAGAGA TCTGGAAGCC ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CTTTGGCTGC TGGGCACCCA GCCCAAGCCC CTGCCATCAA CACACGGCTT 
GGAGAGGTTC AAACTGGATG GGAGGATCTT CGGGCAACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGACGGCT GCAAGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACTGC TGTAGCCTCT GAGGAAGGAC CAGCCACCCT TCCAGAGGCA 
GAAGCCCTCT TAGCCCAGCA TGCAGCTCTG CGGGGAGAGG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAATGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CTTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTGAGAGAT 
GCTCGCCAGG CTGAGGGAGT TCTCAGCAGC CAGGAATATG TTCTATCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC GGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACC 
ATGGACGCCA ATGGAGAGCG CATCCGTGGA CTCCTGGAGG CTGGCCGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCAG ACTCCATCGA GAAGAGGCAC 
AGAAAGAACC AGGAGGCCGT GCAGCAGCTT CTAGGCCGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AAACTCTGGA TTGACGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA TGAGGCACGC AACCTGCACA CCAAGTGGCA AAAACACCAG 
GCATTCATGG CCGAGCTGGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCCTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACAGGC GCTGGGATGA ACTGGAGACT ACCACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCTAACC GGGCAGAGCT ATTTGCCCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAGG ACCTCACCAG TGTCAACATT 
CTGCTAAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCTATCCAGG CCCAGGCAAA AGCCCTGGCC CAGGAAGACC AAAGTGCAGG AGAGGTGGAA 
AGGACCTCCA GAGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAGCCCAT GAAGGACCGC 
TGCCGGCGCC TGCAAGCCTC CCGAGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATACTGTGGG TGACCGAGCG GCTTCCCATG GCCAGCTCTC TGGAGCATGG CAAGGACTTG 
CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACTCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGA CAGCGCACTC TGGGAACAGC AGCAGCGGGT 
CCAGAGCTGG CTGAGCTCCA GGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGTAAACGAC TGGAGGAGGC CCTTCGAGCC CAGCAATTCT ATCGTGACGC TGCAGAGGCC 
GAGGCTTGGA TGGGGGAGCA GGAGTTACAT ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTACTAG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GGTTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTAAAG 
GAGCTGGCAG GAGAGCGGCG TGAGCGTCTG CAGGAGCACC TCAGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA GCAGTGGATA CAGGAGCGAG AAGTCGTGGC AGCCTCCCAT 
GAACTGGGCC AGGACTATGA GCATGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AGGGACACCA GCACCATTGG CCAAGAGCGT GTAGACAGTG CCAATGCCCT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCT 
TATGAGCTGC AGCGCTTCCT GCATGGGGCC CGCCAAGCCC TGGCACGGGT GCAGCACAAG 
CAGCAGCAGC TTCCAGATGG GACGGGCCGC GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGCG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAGGTTCAG 
GACGATGGCC TCAGGCTACA AAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCGTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGTCGC 
CAGCTGTTAC TGGATACCAC AGACAAATTC CGATTCTTCA AGGCTGTCCG GGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGAGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGCATCAAAG CAGAGATAGA GGCAAGAGCT 
GACAGGTTCT CCGCCTGCAT TGACATGGGG CAAGAGCTGC TGGCCCGGAA CCACTATGCC 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAA 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGCA TGGCAGAGGC CTGGCTATGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGATGA AGTAGAGAGC CTCATCAAGC GGCATGAAGC CTTCCAGAAG 
TCAGCAGTGG CCTGGGAGGA GCGTTTCAGT GCCCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAGAATG AGCAGAAAAG GAAGAGGGAG GAGGAGGAAC GAAGGAAACA GCCCCCTACT 
TCAGAGCCCA TGGCTAGTCA ACCGGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCTTGAC 
ACTGCCTGGG ATGGGACCCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGCATTAAT 
GGGGTCTGCA CGGACACGGA GTCCTCACAG CCCCTGTTGG AACAGCAAAG ACTTGAACAG 
AGCAATGTCC CAGAAGGGCC TGGATCTGGC ACAGGAGACG AGTCCAGCGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCGTCTCCAA TGCCCCAGAG CAGATCGTCT 
GAGTCAGCTC ATGTTGCCAC CCTGCCCGCA CGAGGTGCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA CGCTGTGCCG CAAACAGGAG ATGGAAGCCT TCAATAAGAA AGCTGCCAAC 
AGGTCCTGGC AGAATGTGTA CTGTGTACTT CGGCGTGGAA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCATAC CATGGAGAAG TGCCTGTCAG TCTGGCCAGG 
GCCCAGGGCA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTCTTCAA GCTGGGCTTG 
CAGGATGGGA AAGAGTATCT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCG TCCTCGGCCT CTGGAGAGCC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC AGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGCT CCATCGTGCT TCGCAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062040.2
CDS	391..7557
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLREMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVSDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQDLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVLVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAKALAQEDQSAGEVERTSRAVEEKFRALCQPMKDRCRRLQASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYAGLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGLRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSACIDMGQELLARNHYAAEEISEKLSQLQSRRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENEQKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVLDTAWDGTQSKLPPSTQAPSINGVCTDTESSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSESAHVATLPARGAELSAQEQMEGTLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSASGEPEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	NM_019167.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019167.2

ORF Insert Sequence:

ATGAGCAGCA CCCTGTCACC CACTGACTTC GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCCGAC TCAGATTGGG ACAATGACAG CAGTTCAGCC 
CGCCTCTTTG AGAGGTCCAG AATTAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCCAC CAAGGGCCGG ATGCGGATTC ACTGCCTGGA GAATGTCGAC 
AAAGCACTGC AGTTCCTGAA GGAGCAGAAG GTGCACCTGG AAAACATGGG CTCCCACGAC 
ATTGTGGATG GGAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGGTATC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAACGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAC 
GTAGACCAAC CCGATGAGAA GTCCATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCGAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGGATTG GCAAGGTCCT GGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAT GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATCGAGCAAA CGATTGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGCGTC 
CAGAACCAGC TGCAGTCTTT CAATTCCTAC CGCACGGTGG AGAAGCCACC CAAGTTCACA 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GTAAGCTGCG GGCCAACAAC 
CAGAAAGTCT ACACACCACG CGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTAGAGA AAGCCGAACA TGAGCGAGAG CTGGCCCTGC GCACGGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGCCT CGTCTCCCAG GACAACTTTG GCCTGGAGCT GGCAGCAGTG 
GAGGCAGCAG TGCGGAAGCA TGAAGCCATT GAGACAGACA TTGTGGCCTA CAGCGGCCGG 
GTGCAAGCGG TGGACGCCGT AGCCGCAGAA CTGGCCGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CGCGGCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG AGAGATGGTG 
GCCGCCCGCC GTGAGCGGCT CCTTCTCAAC CTGGAGCTGC AGAAGGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTAGGC 
AAGCATCTGG CTGGAGTGGA AGATCTACTG CAACTACACG AACTGGTGGA GGCGGACATT 
GCAGTTCAGG CTGAGAGGGT GCGAGCGGTC AGCGCCTCTG CCCTGCGCTT CTGCGACCCC 
GGGAAAGAGT ATAGACCATG TGACCCACAG CTAGTGTCAG AGAGGGTAGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG CGAATTGGCA GCAACTCGAA GGGCCCGACT GGAAGAGTCC 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCCG AGGCCTGGGT TCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGAGACAGGC CGGGACCTGA CTGGTGTCCT CCGCCTGCTC 
AATAAGCACA CAGCCCTACG GGGTGAGATG AGTGGCCGCC TGGGGCCCCT GAAGCTCACC 
CTGGAACAAG GTCAGCAGTT AGTTGCCGAG GGCCACCCTG GAGCTAACCA AGCCTCAACC 
CGTGCAGCAG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCGGCTAG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCCAA TGACATGGAG 
GCTTGGTTGG TGGACGCACT ACGCCTGGTA TCTAGCCCTG AGGTAGGGCA CGATGAGTTC 
TCCACGCAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAGG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCTTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCTGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGG 
GCAGGTGAGC GTGCACGAGC CCTGGAAGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCCGGGGCTT GTGGGCTCTG GGTAGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAACAGAGGT TTGAGACCTT AGAGCCCGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAGTGACA TAGCTGAGCA GTTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTCA GGTCCCTGGC AGATGGCAAA AAGGCAGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAAAA GACCAAGGTC 
ATTGAGTCTA CCCAGGACCT AGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
CTGGCAGGCA CTGAGAGAGA TCTGGAAGCC ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CTTTGGCTGC TGGGCACCCA GCCCAAGCCC CTGCCATCAA CACACGGCTT 
GGAGAGGTTC AAACTGGATG GGAGGATCTT CGGGCAACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGACGGCT GCAAGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACTGC TGTAGCCTCT GAGGAAGGAC CAGCCACCCT TCCAGAGGCA 
GAAGCCCTCT TAGCCCAGCA TGCAGCTCTG CGGGGAGAGG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAATGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CTTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTGAGAGAT 
GCTCGCCAGG CTGAGGGAGT TCTCAGCAGC CAGGAATATG TTCTATCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC GGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACC 
ATGGACGCCA ATGGAGAGCG CATCCGTGGA CTCCTGGAGG CTGGCCGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCAG ACTCCATCGA GAAGAGGCAC 
AGAAAGAACC AGGAGGCCGT GCAGCAGCTT CTAGGCCGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AAACTCTGGA TTGACGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA TGAGGCACGC AACCTGCACA CCAAGTGGCA AAAACACCAG 
GCATTCATGG CCGAGCTGGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCCTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACAGGC GCTGGGATGA ACTGGAGACT ACCACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCTAACC GGGCAGAGCT ATTTGCCCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAGG ACCTCACCAG TGTCAACATT 
CTGCTAAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCTATCCAGG CCCAGGCAAA AGCCCTGGCC CAGGAAGACC AAAGTGCAGG AGAGGTGGAA 
AGGACCTCCA GAGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAGCCCAT GAAGGACCGC 
TGCCGGCGCC TGCAAGCCTC CCGAGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATACTGTGGG TGACCGAGCG GCTTCCCATG GCCAGCTCTC TGGAGCATGG CAAGGACTTG 
CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACTCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGA CAGCGCACTC TGGGAACAGC AGCAGCGGGT 
CCAGAGCTGG CTGAGCTCCA GGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGTAAACGAC TGGAGGAGGC CCTTCGAGCC CAGCAATTCT ATCGTGACGC TGCAGAGGCC 
GAGGCTTGGA TGGGGGAGCA GGAGTTACAT ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTACTAG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GGTTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTAAAG 
GAGCTGGCAG GAGAGCGGCG TGAGCGTCTG CAGGAGCACC TCAGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA GCAGTGGATA CAGGAGCGAG AAGTCGTGGC AGCCTCCCAT 
GAACTGGGCC AGGACTATGA GCATGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AGGGACACCA GCACCATTGG CCAAGAGCGT GTAGACAGTG CCAATGCCCT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCT 
TATGAGCTGC AGCGCTTCCT GCATGGGGCC CGCCAAGCCC TGGCACGGGT GCAGCACAAG 
CAGCAGCAGC TTCCAGATGG GACGGGCCGC GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGCG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAGGTTCAG 
GACGATGGCC TCAGGCTACA AAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCGTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGTCGC 
CAGCTGTTAC TGGATACCAC AGACAAATTC CGATTCTTCA AGGCTGTCCG GGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGAGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGCATCAAAG CAGAGATAGA GGCAAGAGCT 
GACAGGTTCT CCGCCTGCAT TGACATGGGG CAAGAGCTGC TGGCCCGGAA CCACTATGCC 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAA 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGCA TGGCAGAGGC CTGGCTATGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGATGA AGTAGAGAGC CTCATCAAGC GGCATGAAGC CTTCCAGAAG 
TCAGCAGTGG CCTGGGAGGA GCGTTTCAGT GCCCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAGAATG AGCAGAAAAG GAAGAGGGAG GAGGAGGAAC GAAGGAAACA GCCCCCTACT 
TCAGAGCCCA TGGCTAGTCA ACCGGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCTTGAC 
ACTGCCTGGG ATGGGACCCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGCATTAAT 
GGGGTCTGCA CGGACACGGA GTCCTCACAG CCCCTGTTGG AACAGCAAAG ACTTGAACAG 
AGCAATGTCC CAGAAGGGCC TGGATCTGGC ACAGGAGACG AGTCCAGCGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCGTCTCCAA TGCCCCAGAG CAGATCGTCT 
GAGTCAGCTC ATGTTGCCAC CCTGCCCGCA CGAGGTGCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA CGCTGTGCCG CAAACAGGAG ATGGAAGCCT TCAATAAGAA AGCTGCCAAC 
AGGTCCTGGC AGAATGTGTA CTGTGTACTT CGGCGTGGAA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCATAC CATGGAGAAG TGCCTGTCAG TCTGGCCAGG 
GCCCAGGGCA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTCTTCAA GCTGGGCTTG 
CAGGATGGGA AAGAGTATCT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCG TCCTCGGCCT CTGGAGAGCC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC AGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGCT CCATCGTGCT TCGCAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_008760094.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758316.1 Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.

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CloneID	ORa23710
Clone ID Related Accession (Same CDS sequence)	NM_019167.2 , XM_008760094.2 , XM_017588856.1
Accession Version	XM_008760094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CCCTGTCACC CACTGACTTC GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCCGAC TCAGATTGGG ACAATGACAG CAGTTCAGCC 
CGCCTCTTTG AGAGGTCCAG AATTAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCCAC CAAGGGCCGG ATGCGGATTC ACTGCCTGGA GAATGTCGAC 
AAAGCACTGC AGTTCCTGAA GGAGCAGAAG GTGCACCTGG AAAACATGGG CTCCCACGAC 
ATTGTGGATG GGAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGGTATC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAACGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAC 
GTAGACCAAC CCGATGAGAA GTCCATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCGAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGGATTG GCAAGGTCCT GGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAT GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATCGAGCAAA CGATTGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGCGTC 
CAGAACCAGC TGCAGTCTTT CAATTCCTAC CGCACGGTGG AGAAGCCACC CAAGTTCACA 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GTAAGCTGCG GGCCAACAAC 
CAGAAAGTCT ACACACCACG CGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTAGAGA AAGCCGAACA TGAGCGAGAG CTGGCCCTGC GCACGGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGCCT CGTCTCCCAG GACAACTTTG GCCTGGAGCT GGCAGCAGTG 
GAGGCAGCAG TGCGGAAGCA TGAAGCCATT GAGACAGACA TTGTGGCCTA CAGCGGCCGG 
GTGCAAGCGG TGGACGCCGT AGCCGCAGAA CTGGCCGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CGCGGCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG AGAGATGGTG 
GCCGCCCGCC GTGAGCGGCT CCTTCTCAAC CTGGAGCTGC AGAAGGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTAGGC 
AAGCATCTGG CTGGAGTGGA AGATCTACTG CAACTACACG AACTGGTGGA GGCGGACATT 
GCAGTTCAGG CTGAGAGGGT GCGAGCGGTC AGCGCCTCTG CCCTGCGCTT CTGCGACCCC 
GGGAAAGAGT ATAGACCATG TGACCCACAG CTAGTGTCAG AGAGGGTAGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG CGAATTGGCA GCAACTCGAA GGGCCCGACT GGAAGAGTCC 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCCG AGGCCTGGGT TCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGAGACAGGC CGGGACCTGA CTGGTGTCCT CCGCCTGCTC 
AATAAGCACA CAGCCCTACG GGGTGAGATG AGTGGCCGCC TGGGGCCCCT GAAGCTCACC 
CTGGAACAAG GTCAGCAGTT AGTTGCCGAG GGCCACCCTG GAGCTAACCA AGCCTCAACC 
CGTGCAGCAG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCGGCTAG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCCAA TGACATGGAG 
GCTTGGTTGG TGGACGCACT ACGCCTGGTA TCTAGCCCTG AGGTAGGGCA CGATGAGTTC 
TCCACGCAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAGG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCTTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCTGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGG 
GCAGGTGAGC GTGCACGAGC CCTGGAAGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCCGGGGCTT GTGGGCTCTG GGTAGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAACAGAGGT TTGAGACCTT AGAGCCCGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAGTGACA TAGCTGAGCA GTTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTCA GGTCCCTGGC AGATGGCAAA AAGGCAGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAAAA GACCAAGGTC 
ATTGAGTCTA CCCAGGACCT AGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
CTGGCAGGCA CTGAGAGAGA TCTGGAAGCC ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CTTTGGCTGC TGGGCACCCA GCCCAAGCCC CTGCCATCAA CACACGGCTT 
GGAGAGGTTC AAACTGGATG GGAGGATCTT CGGGCAACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGACGGCT GCAAGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACTGC TGTAGCCTCT GAGGAAGGAC CAGCCACCCT TCCAGAGGCA 
GAAGCCCTCT TAGCCCAGCA TGCAGCTCTG CGGGGAGAGG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAATGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CTTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTGAGAGAT 
GCTCGCCAGG CTGAGGGAGT TCTCAGCAGC CAGGAATATG TTCTATCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC GGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACC 
ATGGACGCCA ATGGAGAGCG CATCCGTGGA CTCCTGGAGG CTGGCCGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCAG ACTCCATCGA GAAGAGGCAC 
AGAAAGAACC AGGAGGCCGT GCAGCAGCTT CTAGGCCGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AAACTCTGGA TTGACGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA TGAGGCACGC AACCTGCACA CCAAGTGGCA AAAACACCAG 
GCATTCATGG CCGAGCTGGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCCTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACAGGC GCTGGGATGA ACTGGAGACT ACCACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCTAACC GGGCAGAGCT ATTTGCCCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAGG ACCTCACCAG TGTCAACATT 
CTGCTAAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCTATCCAGG CCCAGGCAAA AGCCCTGGCC CAGGAAGACC AAAGTGCAGG AGAGGTGGAA 
AGGACCTCCA GAGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAGCCCAT GAAGGACCGC 
TGCCGGCGCC TGCAAGCCTC CCGAGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATACTGTGGG TGACCGAGCG GCTTCCCATG GCCAGCTCTC TGGAGCATGG CAAGGACTTG 
CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACTCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGA CAGCGCACTC TGGGAACAGC AGCAGCGGGT 
CCAGAGCTGG CTGAGCTCCA GGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGTAAACGAC TGGAGGAGGC CCTTCGAGCC CAGCAATTCT ATCGTGACGC TGCAGAGGCC 
GAGGCTTGGA TGGGGGAGCA GGAGTTACAT ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTACTAG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GGTTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTAAAG 
GAGCTGGCAG GAGAGCGGCG TGAGCGTCTG CAGGAGCACC TCAGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA GCAGTGGATA CAGGAGCGAG AAGTCGTGGC AGCCTCCCAT 
GAACTGGGCC AGGACTATGA GCATGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AGGGACACCA GCACCATTGG CCAAGAGCGT GTAGACAGTG CCAATGCCCT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCT 
TATGAGCTGC AGCGCTTCCT GCATGGGGCC CGCCAAGCCC TGGCACGGGT GCAGCACAAG 
CAGCAGCAGC TTCCAGATGG GACGGGCCGC GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGCG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAGGTTCAG 
GACGATGGCC TCAGGCTACA AAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCGTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGTCGC 
CAGCTGTTAC TGGATACCAC AGACAAATTC CGATTCTTCA AGGCTGTCCG GGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGAGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGCATCAAAG CAGAGATAGA GGCAAGAGCT 
GACAGGTTCT CCGCCTGCAT TGACATGGGG CAAGAGCTGC TGGCCCGGAA CCACTATGCC 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAA 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGCA TGGCAGAGGC CTGGCTATGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGATGA AGTAGAGAGC CTCATCAAGC GGCATGAAGC CTTCCAGAAG 
TCAGCAGTGG CCTGGGAGGA GCGTTTCAGT GCCCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAGAATG AGCAGAAAAG GAAGAGGGAG GAGGAGGAAC GAAGGAAACA GCCCCCTACT 
TCAGAGCCCA TGGCTAGTCA ACCGGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCTTGAC 
ACTGCCTGGG ATGGGACCCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGCATTAAT 
GGGGTCTGCA CGGACACGGA GTCCTCACAG CCCCTGTTGG AACAGCAAAG ACTTGAACAG 
AGCAATGTCC CAGAAGGGCC TGGATCTGGC ACAGGAGACG AGTCCAGCGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCGTCTCCAA TGCCCCAGAG CAGATCGTCT 
GAGTCAGCTC ATGTTGCCAC CCTGCCCGCA CGAGGTGCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA CGCTGTGCCG CAAACAGGAG ATGGAAGCCT TCAATAAGAA AGCTGCCAAC 
AGGTCCTGGC AGAATGTGTA CTGTGTACTT CGGCGTGGAA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCATAC CATGGAGAAG TGCCTGTCAG TCTGGCCAGG 
GCCCAGGGCA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTCTTCAA GCTGGGCTTG 
CAGGATGGGA AAGAGTATCT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCG TCCTCGGCCT CTGGAGAGCC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC AGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGCT CCATCGTGCT TCGCAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758316.1
CDS	195..7361
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLREMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVSDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQDLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVLVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAKALAQEDQSAGEVERTSRAVEEKFRALCQPMKDRCRRLQASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYAGLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGLRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSACIDMGQELLARNHYAAEEISEKLSQLQSRRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENEQKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVLDTAWDGTQSKLPPSTQAPSINGVCTDTESSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSESAHVATLPARGAELSAQEQMEGTLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSASGEPEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_008760094.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760094.2

ORF Insert Sequence:

ATGAGCAGCA CCCTGTCACC CACTGACTTC GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCCGAC TCAGATTGGG ACAATGACAG CAGTTCAGCC 
CGCCTCTTTG AGAGGTCCAG AATTAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCCAC CAAGGGCCGG ATGCGGATTC ACTGCCTGGA GAATGTCGAC 
AAAGCACTGC AGTTCCTGAA GGAGCAGAAG GTGCACCTGG AAAACATGGG CTCCCACGAC 
ATTGTGGATG GGAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGGTATC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAACGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAC 
GTAGACCAAC CCGATGAGAA GTCCATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCGAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGGATTG GCAAGGTCCT GGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAT GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATCGAGCAAA CGATTGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGCGTC 
CAGAACCAGC TGCAGTCTTT CAATTCCTAC CGCACGGTGG AGAAGCCACC CAAGTTCACA 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GTAAGCTGCG GGCCAACAAC 
CAGAAAGTCT ACACACCACG CGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTAGAGA AAGCCGAACA TGAGCGAGAG CTGGCCCTGC GCACGGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGCCT CGTCTCCCAG GACAACTTTG GCCTGGAGCT GGCAGCAGTG 
GAGGCAGCAG TGCGGAAGCA TGAAGCCATT GAGACAGACA TTGTGGCCTA CAGCGGCCGG 
GTGCAAGCGG TGGACGCCGT AGCCGCAGAA CTGGCCGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CGCGGCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG AGAGATGGTG 
GCCGCCCGCC GTGAGCGGCT CCTTCTCAAC CTGGAGCTGC AGAAGGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTAGGC 
AAGCATCTGG CTGGAGTGGA AGATCTACTG CAACTACACG AACTGGTGGA GGCGGACATT 
GCAGTTCAGG CTGAGAGGGT GCGAGCGGTC AGCGCCTCTG CCCTGCGCTT CTGCGACCCC 
GGGAAAGAGT ATAGACCATG TGACCCACAG CTAGTGTCAG AGAGGGTAGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG CGAATTGGCA GCAACTCGAA GGGCCCGACT GGAAGAGTCC 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCCG AGGCCTGGGT TCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGAGACAGGC CGGGACCTGA CTGGTGTCCT CCGCCTGCTC 
AATAAGCACA CAGCCCTACG GGGTGAGATG AGTGGCCGCC TGGGGCCCCT GAAGCTCACC 
CTGGAACAAG GTCAGCAGTT AGTTGCCGAG GGCCACCCTG GAGCTAACCA AGCCTCAACC 
CGTGCAGCAG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCGGCTAG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCCAA TGACATGGAG 
GCTTGGTTGG TGGACGCACT ACGCCTGGTA TCTAGCCCTG AGGTAGGGCA CGATGAGTTC 
TCCACGCAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAGG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCTTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCTGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGG 
GCAGGTGAGC GTGCACGAGC CCTGGAAGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCCGGGGCTT GTGGGCTCTG GGTAGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAACAGAGGT TTGAGACCTT AGAGCCCGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAGTGACA TAGCTGAGCA GTTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTCA GGTCCCTGGC AGATGGCAAA AAGGCAGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAAAA GACCAAGGTC 
ATTGAGTCTA CCCAGGACCT AGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
CTGGCAGGCA CTGAGAGAGA TCTGGAAGCC ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CTTTGGCTGC TGGGCACCCA GCCCAAGCCC CTGCCATCAA CACACGGCTT 
GGAGAGGTTC AAACTGGATG GGAGGATCTT CGGGCAACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGACGGCT GCAAGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACTGC TGTAGCCTCT GAGGAAGGAC CAGCCACCCT TCCAGAGGCA 
GAAGCCCTCT TAGCCCAGCA TGCAGCTCTG CGGGGAGAGG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAATGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CTTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTGAGAGAT 
GCTCGCCAGG CTGAGGGAGT TCTCAGCAGC CAGGAATATG TTCTATCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC GGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACC 
ATGGACGCCA ATGGAGAGCG CATCCGTGGA CTCCTGGAGG CTGGCCGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCAG ACTCCATCGA GAAGAGGCAC 
AGAAAGAACC AGGAGGCCGT GCAGCAGCTT CTAGGCCGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AAACTCTGGA TTGACGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA TGAGGCACGC AACCTGCACA CCAAGTGGCA AAAACACCAG 
GCATTCATGG CCGAGCTGGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCCTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACAGGC GCTGGGATGA ACTGGAGACT ACCACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCTAACC GGGCAGAGCT ATTTGCCCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAGG ACCTCACCAG TGTCAACATT 
CTGCTAAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCTATCCAGG CCCAGGCAAA AGCCCTGGCC CAGGAAGACC AAAGTGCAGG AGAGGTGGAA 
AGGACCTCCA GAGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAGCCCAT GAAGGACCGC 
TGCCGGCGCC TGCAAGCCTC CCGAGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATACTGTGGG TGACCGAGCG GCTTCCCATG GCCAGCTCTC TGGAGCATGG CAAGGACTTG 
CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACTCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGA CAGCGCACTC TGGGAACAGC AGCAGCGGGT 
CCAGAGCTGG CTGAGCTCCA GGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGTAAACGAC TGGAGGAGGC CCTTCGAGCC CAGCAATTCT ATCGTGACGC TGCAGAGGCC 
GAGGCTTGGA TGGGGGAGCA GGAGTTACAT ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTACTAG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GGTTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTAAAG 
GAGCTGGCAG GAGAGCGGCG TGAGCGTCTG CAGGAGCACC TCAGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA GCAGTGGATA CAGGAGCGAG AAGTCGTGGC AGCCTCCCAT 
GAACTGGGCC AGGACTATGA GCATGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AGGGACACCA GCACCATTGG CCAAGAGCGT GTAGACAGTG CCAATGCCCT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCT 
TATGAGCTGC AGCGCTTCCT GCATGGGGCC CGCCAAGCCC TGGCACGGGT GCAGCACAAG 
CAGCAGCAGC TTCCAGATGG GACGGGCCGC GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGCG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAGGTTCAG 
GACGATGGCC TCAGGCTACA AAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCGTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGTCGC 
CAGCTGTTAC TGGATACCAC AGACAAATTC CGATTCTTCA AGGCTGTCCG GGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGAGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGCATCAAAG CAGAGATAGA GGCAAGAGCT 
GACAGGTTCT CCGCCTGCAT TGACATGGGG CAAGAGCTGC TGGCCCGGAA CCACTATGCC 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAA 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGCA TGGCAGAGGC CTGGCTATGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGATGA AGTAGAGAGC CTCATCAAGC GGCATGAAGC CTTCCAGAAG 
TCAGCAGTGG CCTGGGAGGA GCGTTTCAGT GCCCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAGAATG AGCAGAAAAG GAAGAGGGAG GAGGAGGAAC GAAGGAAACA GCCCCCTACT 
TCAGAGCCCA TGGCTAGTCA ACCGGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCTTGAC 
ACTGCCTGGG ATGGGACCCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGCATTAAT 
GGGGTCTGCA CGGACACGGA GTCCTCACAG CCCCTGTTGG AACAGCAAAG ACTTGAACAG 
AGCAATGTCC CAGAAGGGCC TGGATCTGGC ACAGGAGACG AGTCCAGCGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCGTCTCCAA TGCCCCAGAG CAGATCGTCT 
GAGTCAGCTC ATGTTGCCAC CCTGCCCGCA CGAGGTGCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA CGCTGTGCCG CAAACAGGAG ATGGAAGCCT TCAATAAGAA AGCTGCCAAC 
AGGTCCTGGC AGAATGTGTA CTGTGTACTT CGGCGTGGAA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCATAC CATGGAGAAG TGCCTGTCAG TCTGGCCAGG 
GCCCAGGGCA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTCTTCAA GCTGGGCTTG 
CAGGATGGGA AAGAGTATCT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCG TCCTCGGCCT CTGGAGAGCC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC AGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGCT CCATCGTGCT TCGCAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_017588856.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444345.1 Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.

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CloneID	ORa23710
Clone ID Related Accession (Same CDS sequence)	NM_019167.2 , XM_008760094.2 , XM_017588856.1
Accession Version	XM_017588856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CCCTGTCACC CACTGACTTC GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCCGAC TCAGATTGGG ACAATGACAG CAGTTCAGCC 
CGCCTCTTTG AGAGGTCCAG AATTAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCCAC CAAGGGCCGG ATGCGGATTC ACTGCCTGGA GAATGTCGAC 
AAAGCACTGC AGTTCCTGAA GGAGCAGAAG GTGCACCTGG AAAACATGGG CTCCCACGAC 
ATTGTGGATG GGAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGGTATC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAACGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAC 
GTAGACCAAC CCGATGAGAA GTCCATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCGAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGGATTG GCAAGGTCCT GGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAT GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATCGAGCAAA CGATTGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGCGTC 
CAGAACCAGC TGCAGTCTTT CAATTCCTAC CGCACGGTGG AGAAGCCACC CAAGTTCACA 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GTAAGCTGCG GGCCAACAAC 
CAGAAAGTCT ACACACCACG CGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTAGAGA AAGCCGAACA TGAGCGAGAG CTGGCCCTGC GCACGGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGCCT CGTCTCCCAG GACAACTTTG GCCTGGAGCT GGCAGCAGTG 
GAGGCAGCAG TGCGGAAGCA TGAAGCCATT GAGACAGACA TTGTGGCCTA CAGCGGCCGG 
GTGCAAGCGG TGGACGCCGT AGCCGCAGAA CTGGCCGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CGCGGCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG AGAGATGGTG 
GCCGCCCGCC GTGAGCGGCT CCTTCTCAAC CTGGAGCTGC AGAAGGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTAGGC 
AAGCATCTGG CTGGAGTGGA AGATCTACTG CAACTACACG AACTGGTGGA GGCGGACATT 
GCAGTTCAGG CTGAGAGGGT GCGAGCGGTC AGCGCCTCTG CCCTGCGCTT CTGCGACCCC 
GGGAAAGAGT ATAGACCATG TGACCCACAG CTAGTGTCAG AGAGGGTAGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG CGAATTGGCA GCAACTCGAA GGGCCCGACT GGAAGAGTCC 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCCG AGGCCTGGGT TCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGAGACAGGC CGGGACCTGA CTGGTGTCCT CCGCCTGCTC 
AATAAGCACA CAGCCCTACG GGGTGAGATG AGTGGCCGCC TGGGGCCCCT GAAGCTCACC 
CTGGAACAAG GTCAGCAGTT AGTTGCCGAG GGCCACCCTG GAGCTAACCA AGCCTCAACC 
CGTGCAGCAG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCGGCTAG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCCAA TGACATGGAG 
GCTTGGTTGG TGGACGCACT ACGCCTGGTA TCTAGCCCTG AGGTAGGGCA CGATGAGTTC 
TCCACGCAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAGG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCTTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCTGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGG 
GCAGGTGAGC GTGCACGAGC CCTGGAAGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCCGGGGCTT GTGGGCTCTG GGTAGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAACAGAGGT TTGAGACCTT AGAGCCCGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAGTGACA TAGCTGAGCA GTTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTCA GGTCCCTGGC AGATGGCAAA AAGGCAGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAAAA GACCAAGGTC 
ATTGAGTCTA CCCAGGACCT AGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
CTGGCAGGCA CTGAGAGAGA TCTGGAAGCC ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CTTTGGCTGC TGGGCACCCA GCCCAAGCCC CTGCCATCAA CACACGGCTT 
GGAGAGGTTC AAACTGGATG GGAGGATCTT CGGGCAACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGACGGCT GCAAGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACTGC TGTAGCCTCT GAGGAAGGAC CAGCCACCCT TCCAGAGGCA 
GAAGCCCTCT TAGCCCAGCA TGCAGCTCTG CGGGGAGAGG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAATGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CTTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTGAGAGAT 
GCTCGCCAGG CTGAGGGAGT TCTCAGCAGC CAGGAATATG TTCTATCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC GGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACC 
ATGGACGCCA ATGGAGAGCG CATCCGTGGA CTCCTGGAGG CTGGCCGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCAG ACTCCATCGA GAAGAGGCAC 
AGAAAGAACC AGGAGGCCGT GCAGCAGCTT CTAGGCCGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AAACTCTGGA TTGACGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA TGAGGCACGC AACCTGCACA CCAAGTGGCA AAAACACCAG 
GCATTCATGG CCGAGCTGGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCCTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACAGGC GCTGGGATGA ACTGGAGACT ACCACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCTAACC GGGCAGAGCT ATTTGCCCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAGG ACCTCACCAG TGTCAACATT 
CTGCTAAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCTATCCAGG CCCAGGCAAA AGCCCTGGCC CAGGAAGACC AAAGTGCAGG AGAGGTGGAA 
AGGACCTCCA GAGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAGCCCAT GAAGGACCGC 
TGCCGGCGCC TGCAAGCCTC CCGAGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATACTGTGGG TGACCGAGCG GCTTCCCATG GCCAGCTCTC TGGAGCATGG CAAGGACTTG 
CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACTCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGA CAGCGCACTC TGGGAACAGC AGCAGCGGGT 
CCAGAGCTGG CTGAGCTCCA GGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGTAAACGAC TGGAGGAGGC CCTTCGAGCC CAGCAATTCT ATCGTGACGC TGCAGAGGCC 
GAGGCTTGGA TGGGGGAGCA GGAGTTACAT ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTACTAG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GGTTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTAAAG 
GAGCTGGCAG GAGAGCGGCG TGAGCGTCTG CAGGAGCACC TCAGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA GCAGTGGATA CAGGAGCGAG AAGTCGTGGC AGCCTCCCAT 
GAACTGGGCC AGGACTATGA GCATGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AGGGACACCA GCACCATTGG CCAAGAGCGT GTAGACAGTG CCAATGCCCT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCT 
TATGAGCTGC AGCGCTTCCT GCATGGGGCC CGCCAAGCCC TGGCACGGGT GCAGCACAAG 
CAGCAGCAGC TTCCAGATGG GACGGGCCGC GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGCG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAGGTTCAG 
GACGATGGCC TCAGGCTACA AAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCGTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGTCGC 
CAGCTGTTAC TGGATACCAC AGACAAATTC CGATTCTTCA AGGCTGTCCG GGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGAGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGCATCAAAG CAGAGATAGA GGCAAGAGCT 
GACAGGTTCT CCGCCTGCAT TGACATGGGG CAAGAGCTGC TGGCCCGGAA CCACTATGCC 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAA 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGCA TGGCAGAGGC CTGGCTATGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGATGA AGTAGAGAGC CTCATCAAGC GGCATGAAGC CTTCCAGAAG 
TCAGCAGTGG CCTGGGAGGA GCGTTTCAGT GCCCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAGAATG AGCAGAAAAG GAAGAGGGAG GAGGAGGAAC GAAGGAAACA GCCCCCTACT 
TCAGAGCCCA TGGCTAGTCA ACCGGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCTTGAC 
ACTGCCTGGG ATGGGACCCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGCATTAAT 
GGGGTCTGCA CGGACACGGA GTCCTCACAG CCCCTGTTGG AACAGCAAAG ACTTGAACAG 
AGCAATGTCC CAGAAGGGCC TGGATCTGGC ACAGGAGACG AGTCCAGCGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCGTCTCCAA TGCCCCAGAG CAGATCGTCT 
GAGTCAGCTC ATGTTGCCAC CCTGCCCGCA CGAGGTGCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA CGCTGTGCCG CAAACAGGAG ATGGAAGCCT TCAATAAGAA AGCTGCCAAC 
AGGTCCTGGC AGAATGTGTA CTGTGTACTT CGGCGTGGAA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCATAC CATGGAGAAG TGCCTGTCAG TCTGGCCAGG 
GCCCAGGGCA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTCTTCAA GCTGGGCTTG 
CAGGATGGGA AAGAGTATCT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCG TCCTCGGCCT CTGGAGAGCC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC AGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGCT CCATCGTGCT TCGCAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444345.1
CDS	267..7433
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLREMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVSDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQDLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVLVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAKALAQEDQSAGEVERTSRAVEEKFRALCQPMKDRCRRLQASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYAGLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGLRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSACIDMGQELLARNHYAAEEISEKLSQLQSRRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENEQKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVLDTAWDGTQSKLPPSTQAPSINGVCTDTESSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSESAHVATLPARGAELSAQEQMEGTLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSASGEPEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_017588856.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588856.1

ORF Insert Sequence:

ATGAGCAGCA CCCTGTCACC CACTGACTTC GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCCGAC TCAGATTGGG ACAATGACAG CAGTTCAGCC 
CGCCTCTTTG AGAGGTCCAG AATTAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCCAC CAAGGGCCGG ATGCGGATTC ACTGCCTGGA GAATGTCGAC 
AAAGCACTGC AGTTCCTGAA GGAGCAGAAG GTGCACCTGG AAAACATGGG CTCCCACGAC 
ATTGTGGATG GGAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGGTATC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAACGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAC 
GTAGACCAAC CCGATGAGAA GTCCATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCGAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGGATTG GCAAGGTCCT GGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAT GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATCGAGCAAA CGATTGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGCGTC 
CAGAACCAGC TGCAGTCTTT CAATTCCTAC CGCACGGTGG AGAAGCCACC CAAGTTCACA 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GTAAGCTGCG GGCCAACAAC 
CAGAAAGTCT ACACACCACG CGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTAGAGA AAGCCGAACA TGAGCGAGAG CTGGCCCTGC GCACGGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGCCT CGTCTCCCAG GACAACTTTG GCCTGGAGCT GGCAGCAGTG 
GAGGCAGCAG TGCGGAAGCA TGAAGCCATT GAGACAGACA TTGTGGCCTA CAGCGGCCGG 
GTGCAAGCGG TGGACGCCGT AGCCGCAGAA CTGGCCGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CGCGGCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG AGAGATGGTG 
GCCGCCCGCC GTGAGCGGCT CCTTCTCAAC CTGGAGCTGC AGAAGGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTAGGC 
AAGCATCTGG CTGGAGTGGA AGATCTACTG CAACTACACG AACTGGTGGA GGCGGACATT 
GCAGTTCAGG CTGAGAGGGT GCGAGCGGTC AGCGCCTCTG CCCTGCGCTT CTGCGACCCC 
GGGAAAGAGT ATAGACCATG TGACCCACAG CTAGTGTCAG AGAGGGTAGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG CGAATTGGCA GCAACTCGAA GGGCCCGACT GGAAGAGTCC 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCCG AGGCCTGGGT TCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGAGACAGGC CGGGACCTGA CTGGTGTCCT CCGCCTGCTC 
AATAAGCACA CAGCCCTACG GGGTGAGATG AGTGGCCGCC TGGGGCCCCT GAAGCTCACC 
CTGGAACAAG GTCAGCAGTT AGTTGCCGAG GGCCACCCTG GAGCTAACCA AGCCTCAACC 
CGTGCAGCAG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCGGCTAG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCCAA TGACATGGAG 
GCTTGGTTGG TGGACGCACT ACGCCTGGTA TCTAGCCCTG AGGTAGGGCA CGATGAGTTC 
TCCACGCAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAGG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCTTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCTGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGG 
GCAGGTGAGC GTGCACGAGC CCTGGAAGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCCGGGGCTT GTGGGCTCTG GGTAGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAACAGAGGT TTGAGACCTT AGAGCCCGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAGTGACA TAGCTGAGCA GTTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTCA GGTCCCTGGC AGATGGCAAA AAGGCAGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAAAA GACCAAGGTC 
ATTGAGTCTA CCCAGGACCT AGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
CTGGCAGGCA CTGAGAGAGA TCTGGAAGCC ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CTTTGGCTGC TGGGCACCCA GCCCAAGCCC CTGCCATCAA CACACGGCTT 
GGAGAGGTTC AAACTGGATG GGAGGATCTT CGGGCAACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGACGGCT GCAAGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACTGC TGTAGCCTCT GAGGAAGGAC CAGCCACCCT TCCAGAGGCA 
GAAGCCCTCT TAGCCCAGCA TGCAGCTCTG CGGGGAGAGG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAATGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CTTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTGAGAGAT 
GCTCGCCAGG CTGAGGGAGT TCTCAGCAGC CAGGAATATG TTCTATCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC GGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACC 
ATGGACGCCA ATGGAGAGCG CATCCGTGGA CTCCTGGAGG CTGGCCGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCAG ACTCCATCGA GAAGAGGCAC 
AGAAAGAACC AGGAGGCCGT GCAGCAGCTT CTAGGCCGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AAACTCTGGA TTGACGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA TGAGGCACGC AACCTGCACA CCAAGTGGCA AAAACACCAG 
GCATTCATGG CCGAGCTGGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCCTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACAGGC GCTGGGATGA ACTGGAGACT ACCACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCTAACC GGGCAGAGCT ATTTGCCCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAGG ACCTCACCAG TGTCAACATT 
CTGCTAAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCTATCCAGG CCCAGGCAAA AGCCCTGGCC CAGGAAGACC AAAGTGCAGG AGAGGTGGAA 
AGGACCTCCA GAGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAGCCCAT GAAGGACCGC 
TGCCGGCGCC TGCAAGCCTC CCGAGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATACTGTGGG TGACCGAGCG GCTTCCCATG GCCAGCTCTC TGGAGCATGG CAAGGACTTG 
CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACTCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGA CAGCGCACTC TGGGAACAGC AGCAGCGGGT 
CCAGAGCTGG CTGAGCTCCA GGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGTAAACGAC TGGAGGAGGC CCTTCGAGCC CAGCAATTCT ATCGTGACGC TGCAGAGGCC 
GAGGCTTGGA TGGGGGAGCA GGAGTTACAT ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTACTAG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GGTTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTAAAG 
GAGCTGGCAG GAGAGCGGCG TGAGCGTCTG CAGGAGCACC TCAGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA GCAGTGGATA CAGGAGCGAG AAGTCGTGGC AGCCTCCCAT 
GAACTGGGCC AGGACTATGA GCATGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AGGGACACCA GCACCATTGG CCAAGAGCGT GTAGACAGTG CCAATGCCCT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCT 
TATGAGCTGC AGCGCTTCCT GCATGGGGCC CGCCAAGCCC TGGCACGGGT GCAGCACAAG 
CAGCAGCAGC TTCCAGATGG GACGGGCCGC GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGCG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAGGTTCAG 
GACGATGGCC TCAGGCTACA AAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCGTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGTCGC 
CAGCTGTTAC TGGATACCAC AGACAAATTC CGATTCTTCA AGGCTGTCCG GGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGAGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGCATCAAAG CAGAGATAGA GGCAAGAGCT 
GACAGGTTCT CCGCCTGCAT TGACATGGGG CAAGAGCTGC TGGCCCGGAA CCACTATGCC 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAA 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGCA TGGCAGAGGC CTGGCTATGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGATGA AGTAGAGAGC CTCATCAAGC GGCATGAAGC CTTCCAGAAG 
TCAGCAGTGG CCTGGGAGGA GCGTTTCAGT GCCCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAGAATG AGCAGAAAAG GAAGAGGGAG GAGGAGGAAC GAAGGAAACA GCCCCCTACT 
TCAGAGCCCA TGGCTAGTCA ACCGGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCTTGAC 
ACTGCCTGGG ATGGGACCCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGCATTAAT 
GGGGTCTGCA CGGACACGGA GTCCTCACAG CCCCTGTTGG AACAGCAAAG ACTTGAACAG 
AGCAATGTCC CAGAAGGGCC TGGATCTGGC ACAGGAGACG AGTCCAGCGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCGTCTCCAA TGCCCCAGAG CAGATCGTCT 
GAGTCAGCTC ATGTTGCCAC CCTGCCCGCA CGAGGTGCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA CGCTGTGCCG CAAACAGGAG ATGGAAGCCT TCAATAAGAA AGCTGCCAAC 
AGGTCCTGGC AGAATGTGTA CTGTGTACTT CGGCGTGGAA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCATAC CATGGAGAAG TGCCTGTCAG TCTGGCCAGG 
GCCCAGGGCA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTCTTCAA GCTGGGCTTG 
CAGGATGGGA AAGAGTATCT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCG TCCTCGGCCT CTGGAGAGCC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC AGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGCT CCATCGTGCT TCGCAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_008760092.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758314.1 Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.

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CloneID	ORa43234
Clone ID Related Accession (Same CDS sequence)	XM_008760092.2
Accession Version	XM_008760092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACAA ACGGAGCGGG CAGGGTACAT GGAAATGGCC TGAACGGGGC TGGTGAGTTT 
TACTATGAGG CTGTGGAAGG GTCTCAAAAC CCTGGAGGCA TCCTGCTCTC ACCAGCTGCC 
TTCATCAATC CTGCTCAGTA TGCCAGTGTG CTGGAAGGAC GATTCAAACA GCTTCAAGAT 
GAGCGAGAAG CCGTGCAGAA GAAAACCTTC ACCAAGTGGG TGAACTCCCA CCTGGCCCGG 
GTGACATGCC GGGTGGGAGA CCTGTACAGC GACCTGCGGG ACGGGCGCAA CCTCCTGAGG 
CTCCTGGAGG TGCTCTCGGG AGAGACCCTG CCAAAACCCA CCAAGGGCCG GATGCGGATT 
CACTGCCTGG AGAATGTCGA CAAAGCACTG CAGTTCCTGA AGGAGCAGAA GGTGCACCTG 
GAAAACATGG GCTCCCACGA CATTGTGGAT GGGAACCACC GTCTGACCCT TGGGCTAGTG 
TGGACCATCA TCCTCCGATT TCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG 
GAGAAGAAGT CAGCCAAGGA TGCCCTGCTG CTGTGGTGCC AGATGAAGAC TGCAGGGTAT 
CCCAATGTCA ATGTGCACAA CTTTACCACC AGTTGGAGAG ATGGGCTGGC CTTTAATGCC 
ATTGTGCACA AACACCGGCC AGACCTGTTG GATTTTGAGT CCCTGAAGAA GTGTAACGCA 
CACTACAATC TGCAGAATGC TTTCAATCTG GCTGAAAAGG AACTTGGCCT GACGAAGCTC 
CTGGATCCTG AAGATGTGAA CGTAGACCAA CCCGATGAGA AGTCCATCAT CACCTACGTG 
GCCACTTACT ACCACTACTT CTCGAAGATG AAGGCCCTGG CTGTGGAAGG CAAAAGGATT 
GGCAAGGTCC TGGACCATGC CATGGAGGCA GAACACCTGG TAGAGAAATA TGAGTCCCTG 
GCCTCTGAAC TGCTGCAGTG GATCGAGCAA ACGATTGTGA CCCTCAATGA CCGACAGCTG 
GCCAACTCCC TGAGTGGCGT CCAGAACCAG CTGCAGTCTT TCAATTCCTA CCGCACGGTG 
GAGAAGCCAC CCAAGTTCAC AGAGAAAGGG AACTTGGAGG TGTTGCTCTT CACCATCCAG 
AGTAAGCTGC GGGCCAACAA CCAGAAAGTC TACACACCAC GCGAAGGCCG GCTCATCTCG 
GACATCAACA AGGCCTGGGA GCGGCTAGAG AAAGCCGAAC ATGAGCGAGA GCTGGCCCTG 
CGCACGGAGC TGATCCGCCA GGAGAAGCTG GAGCAACTGG CTGCTCGCTT CGACCGCAAG 
GCTGCCATGC GGGAGACCTG GCTCAGTGAG AACCAGCGCC TCGTCTCCCA GGACAACTTT 
GGCCTGGAGC TGGCAGCAGT GGAGGCAGCA GTGCGGAAGC ATGAAGCCAT TGAGACAGAC 
ATTGTGGCCT ACAGCGGCCG GGTGCAAGCG GTGGACGCCG TAGCCGCAGA ACTGGCCGCT 
GAGCACTACC ATGACATTAA GCGCATTGCG GCGCGGCAGA ACAACGTGGC CCGGCTCTGG 
GACTTCTTAC GAGAGATGGT GGCCGCCCGC CGTGAGCGGC TCCTTCTCAA CCTGGAGCTG 
CAGAAGGTGT TTCAGGACCT GCTCTACCTC ATGGACTGGA TGGCAGAGAT GAAGGGCCGG 
CTGCAGTCTC AGGACCTAGG CAAGCATCTG GCTGGAGTGG AAGATCTACT GCAACTACAC 
GAACTGGTGG AGGCGGACAT TGCAGTTCAG GCTGAGAGGG TGCGAGCGGT CAGCGCCTCT 
GCCCTGCGCT TCTGCGACCC CGGGAAAGAG TATAGACCAT GTGACCCACA GCTAGTGTCA 
GAGAGGGTAG CCACTCTGGA GCAGAGCTAT GAGGCCCTGT GCGAATTGGC AGCAACTCGA 
AGGGCCCGAC TGGAAGAGTC CCGTCGTCTC TGGAGGTTCC TCTGGGAAGT GGGTGAGGCC 
GAGGCCTGGG TTCGGGAGCA GCAGCACCTC CTGGCCTCAG CTGAGACAGG CCGGGACCTG 
ACTGGTGTCC TCCGCCTGCT CAATAAGCAC ACAGCCCTAC GGGGTGAGAT GAGTGGCCGC 
CTGGGGCCCC TGAAGCTCAC CCTGGAACAA GGTCAGCAGT TAGTTGCCGA GGGCCACCCT 
GGAGCTAACC AAGCCTCAAC CCGTGCAGCA GAGCTCCAGG CCCAGTGGGA GCGACTAGAA 
GCCCTGGCCG AGGAGCGAGC CCAGCGGCTA GCACAGGCTG CCAGCCTCTA CCAGTTCCAG 
GCAGATGCCA ATGACATGGA GGCTTGGTTG GTGGACGCAC TACGCCTGGT ATCTAGCCCT 
GAGGTAGGGC ACGATGAGTT CTCCACGCAG GCCCTGGCCA GGCAGCACAG GGCCCTTGAG 
GAGGAGATCC GAGCCCACCG GCCTACACTG GATGCCTTGA GGGAGCAGGC TGCAGCCCTG 
CCACCTGCAC TGAGCCACAC ACCTGAGGTA CAGGGCAGGG TGCCCACTCT GGAGCAGCAC 
TATGAGGAGC TGCAGGCCCG GGCAGGTGAG CGTGCACGAG CCCTGGAAGC AGCCCTGGCG 
TTCTATACCA TGCTCAGCGA GGCCGGGGCT TGTGGGCTCT GGGTAGAGGA GAAGGAGCAG 
TGGCTCAACG GGCTGGCCCT ACCTGAGCGC CTGGAGGACC TGGAGGTGGT CCAACAGAGG 
TTTGAGACCT TAGAGCCCGA AATGAACGCC CTGGCTGCAC GGGTTACTGC TGTCAGTGAC 
ATAGCTGAGC AGTTGCTGAA GGCCAGTCCA CCAGGCAAGG ACCGCATCAT TGGCACCCAG 
GAGCAGCTCA ACCAAAGGTG GCAGCAGTTC AGGTCCCTGG CAGATGGCAA AAAGGCAGCT 
CTGACATCAG CCCTGAGCAT CCAGAATTAC CACCTAGAGT GCACAGAGAC CCAGGCCTGG 
ATGAGAGAAA AGACCAAGGT CATTGAGTCT ACCCAGGACC TAGGCAATGA TCTAGCTGGT 
GTGCTGGCCC TGCAGCGGAA GCTGGCAGGC ACTGAGAGAG ATCTGGAAGC CATCTCTGCC 
CGGGTGGGTG AGCTGACCCA AGAGGCAAAT GCTTTGGCTG CTGGGCACCC AGCCCAAGCC 
CCTGCCATCA ACACACGGCT TGGAGAGGTT CAAACTGGAT GGGAGGATCT TCGGGCAACC 
ATGAGGCGGA GAGAAGAGTC CCTGGGTGAG GCTCGACGGC TGCAAGATTT CCTGCGCAGC 
TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACACAGACTG CTGTAGCCTC TGAGGAAGGA 
CCAGCCACCC TTCCAGAGGC AGAAGCCCTC TTAGCCCAGC ATGCAGCTCT GCGGGGAGAG 
GTGGAGAGAG CCCAGAGCGA GTACAGCCGC CTCAGGACCT TGGGCGAGGA GGTGACCAGA 
GACCAGGCTG ATCCCCAATG CCTCTTCCTC AGACAGAGGC TGGAAGCCCT TGGAACCGGC 
TGGGAGGAGC TGGGTCGCAT GTGGGAGAGC CGGCAAGGCC GCTTGGCCCA GGCCCATGGC 
TTCCAGGGGT TTTTGAGAGA TGCTCGCCAG GCTGAGGGAG TTCTCAGCAG CCAGGAATAT 
GTTCTATCTC ACACGGAGAT GCCAGGGACA CTGCAGGCGG CGGATGCAGC CATTAAAAAG 
CTGGAAGACT TCATGAGCAC CATGGACGCC AATGGAGAGC GCATCCGTGG ACTCCTGGAG 
GCTGGCCGTC AGCTGGTGTC CAAGGGCAAT ATCCATGCTG AGAAGATCCA AGAGAAGGCA 
GACTCCATCG AGAAGAGGCA CAGAAAGAAC CAGGAGGCCG TGCAGCAGCT TCTAGGCCGC 
CTTCGGGACA ACCGAGAGCA GCAGCACTTC TTGCAAGACT GTCAGGAGCT GAAACTCTGG 
ATTGACGAGA AGATGCTGAC AGCTCAGGAT GTGTCCTATG ATGAGGCACG CAACCTGCAC 
ACCAAGTGGC AAAAACACCA GGCATTCATG GCCGAGCTGG CAGCCAACAA GGACTGGCTG 
GACAAAGTGG ACAAGGAAGG GCGGGAGCTG ACTCTTGAAA AGCCAGAACT CAAAGTCCTA 
GTGTCAGAGA AGCTGGAGGA CCTGCACAGG CGCTGGGATG AACTGGAGAC TACCACCCAA 
GCCAAGGCCC GCAGTCTTTT TGATGCTAAC CGGGCAGAGC TATTTGCCCA AAGCTGTTCT 
GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCAGATGA CTATGGCAAG 
GACCTCACCA GTGTCAACAT TCTGCTAAAG AAGCAACAGA TGCTGGAACG AGAGATGGCT 
GTGAGAGAGA AGGAGGTAGA GGCTATCCAG GCCCAGGCAA AAGCCCTGGC CCAGGAAGAC 
CAAAGTGCAG GAGAGGTGGA AAGGACCTCC AGAGCTGTGG AGGAGAAGTT CAGGGCCTTG 
TGTCAGCCCA TGAAGGACCG CTGCCGGCGC CTGCAAGCCT CCCGAGAGCA GCACCAGTTC 
CACCGGGATG TGGAGGATGA GATACTGTGG GTGACCGAGC GGCTTCCCAT GGCCAGCTCT 
CTGGAGCATG GCAAGGACTT GCCCAGCGTC CAGCTTCTCA TGAAGAAAAA CCAGACTCTG 
CAGAAGGAGA TCCAGGGCCA TGAGCCCCGG ATTGCAGACC TCAAAGAGAG ACAGCGCACT 
CTGGGAACAG CAGCAGCGGG TCCAGAGCTG GCTGAGCTCC AGGAAATGTG GAAACGCCTG 
AGCCATGAGC TGGAGCTTCG GGGTAAACGA CTGGAGGAGG CCCTTCGAGC CCAGCAATTC 
TATCGTGACG CTGCAGAGGC CGAGGCTTGG ATGGGGGAGC AGGAGTTACA TATGATGGGC 
CAGGAAAAGG CCAAGGATGA GCTGAGCGCC CAGGCAGAAG TGAAGAAGCA TCAGGTACTA 
GAACAAGCCC TTGCTGACTA TGCCCAGACC ATCAAACAAC TAGCAGCCAG CAGTCAAGAT 
ATGATTGACC ATGAACATCC AGAGAGCACA AGGTTAACAA TACGCCAAGC CCAGGTGGAC 
AAGCTGTATG CCGGCCTAAA GGAGCTGGCA GGAGAGCGGC GTGAGCGTCT GCAGGAGCAC 
CTCAGGCTGT GCCAGCTCCG CAGAGAGCTG GATGACCTGG AGCAGTGGAT ACAGGAGCGA 
GAAGTCGTGG CAGCCTCCCA TGAACTGGGC CAGGACTATG AGCATGTGAC TATGCTTCGG 
GACAAATTCC GAGAGTTCTC CAGGGACACC AGCACCATTG GCCAAGAGCG TGTAGACAGT 
GCCAATGCCC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACCGTGGCC 
GAGTGGAAGG ACAGTCTCAA TGAGGCCTGG GCTGACCTGC TGGAGCTGCT GGACACAAGA 
GGTCAGGTGC TGGCTGCTGC TTATGAGCTG CAGCGCTTCC TGCATGGGGC CCGCCAAGCC 
CTGGCACGGG TGCAGCACAA GCAGCAGCAG CTTCCAGATG GGACGGGCCG CGACCTCAAT 
GCTGCTGAGG CCCTGCAGCG CCGGCACTGC GCCTATGAGC ACGACATCCA AGCCCTCAGC 
ACTCAGGTCC AGCAGGTTCA GGACGATGGC CTCAGGCTAC AAAAGGCCTA TGCTGGAGAC 
AAGGCTGAGG AAATTGGCCG TCACATGCAG GCAGTGGCTG AGGCGTGGGC CCAGCTCCAG 
GGAAGTTCTG CTGCCCGTCG CCAGCTGTTA CTGGATACCA CAGACAAATT CCGATTCTTC 
AAGGCTGTCC GGGAGTTGAT GCTGTGGATG GATGGGATTA ACCTGCAGAT GGATGCCCAG 
GAGAGGCCCC GGGATGTGTC CTCTGCAGAT TTAGTCATCA AAAACCAACA AGGCATCAAA 
GCAGAGATAG AGGCAAGAGC TGACAGGTTC TCCGCCTGCA TTGACATGGG GCAAGAGCTG 
CTGGCCCGGA ACCACTATGC CGCTGAGGAG ATCTCAGAGA AGCTGTCTCA GCTACAGTCC 
CGGCGCCAGG AGACAGCTGA AAAGTGGCAG GAGAAGATGG ACTGGCTACA GCTTGTTTTG 
GAGGTGCTTG TGTTTGGGAG AGATGCAGGC ATGGCAGAGG CCTGGCTATG CAGTCAGGAG 
CCATTGGTGC GAAGTGCAGA ACTGGGTTGC ACTGTGGATG AAGTAGAGAG CCTCATCAAG 
CGGCATGAAG CCTTCCAGAA GTCAGCAGTG GCCTGGGAGG AGCGTTTCAG TGCCCTGGAG 
AAGCTCACTG CGCTGGAAGA GCGGGAGAAT GAGCAGAAAA GGAAGAGGGA GGAGGAGGAA 
CGAAGGAAAC AGCCCCCTAC TTCAGAGCCC ATGGCTAGTC AACCGGAAGG GAGTCTGGTA 
GATGGCCAGA GAGTTCTTGA CACTGCCTGG GATGGGACCC AGTCAAAATT GCCACCATCC 
ACACAAGCAC CCAGCATTAA TGGGGTCTGC ACGGACACGG AGTCCTCACA GCCCCTGTTG 
GAACAGCAAA GACTTGAACA GAGCAATGTC CCAGAAGGGC CTGGATCTGG CACAGGAGAC 
GAGTCCAGCG GGCCCCGGGG AGAGAGGCAG ACCCTGCCCC GGGGCCCTGC TCCGTCTCCA 
ATGCCCCAGA GCAGATCGTC TGAGTCAGCT CATGTTGCCA CCCTGCCCGC ACGAGGTGCT 
GAGCTCTCTG CTCAGGAACA GATGGAAGGG ACGCTGTGCC GCAAACAGGA GATGGAAGCC 
TTCAATAAGA AAGCTGCCAA CAGGTCCTGG CAGAATGTGT ACTGTGTACT TCGGCGTGGA 
AGCCTCGGCT TTTACAAGGA TGCCAGGGCA GCTAGTGCAG GAGTGCCATA CCATGGAGAA 
GTGCCTGTCA GTCTGGCCAG GGCCCAGGGC AGTGTGGCCT TTGATTATCG GAAACGCAAA 
CATGTCTTCA AGCTGGGCTT GCAGGATGGG AAAGAGTATC TATTCCAGGC CAAGGATGAG 
GCAGAGATGA GCTCATGGCT GAGAGTGGTG AATGCAGCCA TTGCCACTGC GTCCTCGGCC 
TCTGGAGAGC CAGAAGAGCC AGTGGTGCCC AGTGCCAGCC GGGGTCTGAC CAGGGCCATG 
ACCATGCCCC CAGTGTCACA GCCTGAGGGC TCCATCGTGC TTCGCAGCAA GGATGGCAGA 
GAAAGAGAGC GAGAAAAACG ATTCAGCTTC TTTAAGAAGA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758314.1
CDS	226..7413
Translation	MSTNGAGRVHGNGLNGAGEFYYEAVEGSQNPGGILLSPAAFINPAQYASVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLREMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVSDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQDLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVLVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAKALAQEDQSAGEVERTSRAVEEKFRALCQPMKDRCRRLQASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYAGLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGLRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSACIDMGQELLARNHYAAEEISEKLSQLQSRRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENEQKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVLDTAWDGTQSKLPPSTQAPSINGVCTDTESSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSESAHVATLPARGAELSAQEQMEGTLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSASGEPEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_008760092.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760092.2

ORF Insert Sequence:

ATGAGTACAA ACGGAGCGGG CAGGGTACAT GGAAATGGCC TGAACGGGGC TGGTGAGTTT 
TACTATGAGG CTGTGGAAGG GTCTCAAAAC CCTGGAGGCA TCCTGCTCTC ACCAGCTGCC 
TTCATCAATC CTGCTCAGTA TGCCAGTGTG CTGGAAGGAC GATTCAAACA GCTTCAAGAT 
GAGCGAGAAG CCGTGCAGAA GAAAACCTTC ACCAAGTGGG TGAACTCCCA CCTGGCCCGG 
GTGACATGCC GGGTGGGAGA CCTGTACAGC GACCTGCGGG ACGGGCGCAA CCTCCTGAGG 
CTCCTGGAGG TGCTCTCGGG AGAGACCCTG CCAAAACCCA CCAAGGGCCG GATGCGGATT 
CACTGCCTGG AGAATGTCGA CAAAGCACTG CAGTTCCTGA AGGAGCAGAA GGTGCACCTG 
GAAAACATGG GCTCCCACGA CATTGTGGAT GGGAACCACC GTCTGACCCT TGGGCTAGTG 
TGGACCATCA TCCTCCGATT TCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG 
GAGAAGAAGT CAGCCAAGGA TGCCCTGCTG CTGTGGTGCC AGATGAAGAC TGCAGGGTAT 
CCCAATGTCA ATGTGCACAA CTTTACCACC AGTTGGAGAG ATGGGCTGGC CTTTAATGCC 
ATTGTGCACA AACACCGGCC AGACCTGTTG GATTTTGAGT CCCTGAAGAA GTGTAACGCA 
CACTACAATC TGCAGAATGC TTTCAATCTG GCTGAAAAGG AACTTGGCCT GACGAAGCTC 
CTGGATCCTG AAGATGTGAA CGTAGACCAA CCCGATGAGA AGTCCATCAT CACCTACGTG 
GCCACTTACT ACCACTACTT CTCGAAGATG AAGGCCCTGG CTGTGGAAGG CAAAAGGATT 
GGCAAGGTCC TGGACCATGC CATGGAGGCA GAACACCTGG TAGAGAAATA TGAGTCCCTG 
GCCTCTGAAC TGCTGCAGTG GATCGAGCAA ACGATTGTGA CCCTCAATGA CCGACAGCTG 
GCCAACTCCC TGAGTGGCGT CCAGAACCAG CTGCAGTCTT TCAATTCCTA CCGCACGGTG 
GAGAAGCCAC CCAAGTTCAC AGAGAAAGGG AACTTGGAGG TGTTGCTCTT CACCATCCAG 
AGTAAGCTGC GGGCCAACAA CCAGAAAGTC TACACACCAC GCGAAGGCCG GCTCATCTCG 
GACATCAACA AGGCCTGGGA GCGGCTAGAG AAAGCCGAAC ATGAGCGAGA GCTGGCCCTG 
CGCACGGAGC TGATCCGCCA GGAGAAGCTG GAGCAACTGG CTGCTCGCTT CGACCGCAAG 
GCTGCCATGC GGGAGACCTG GCTCAGTGAG AACCAGCGCC TCGTCTCCCA GGACAACTTT 
GGCCTGGAGC TGGCAGCAGT GGAGGCAGCA GTGCGGAAGC ATGAAGCCAT TGAGACAGAC 
ATTGTGGCCT ACAGCGGCCG GGTGCAAGCG GTGGACGCCG TAGCCGCAGA ACTGGCCGCT 
GAGCACTACC ATGACATTAA GCGCATTGCG GCGCGGCAGA ACAACGTGGC CCGGCTCTGG 
GACTTCTTAC GAGAGATGGT GGCCGCCCGC CGTGAGCGGC TCCTTCTCAA CCTGGAGCTG 
CAGAAGGTGT TTCAGGACCT GCTCTACCTC ATGGACTGGA TGGCAGAGAT GAAGGGCCGG 
CTGCAGTCTC AGGACCTAGG CAAGCATCTG GCTGGAGTGG AAGATCTACT GCAACTACAC 
GAACTGGTGG AGGCGGACAT TGCAGTTCAG GCTGAGAGGG TGCGAGCGGT CAGCGCCTCT 
GCCCTGCGCT TCTGCGACCC CGGGAAAGAG TATAGACCAT GTGACCCACA GCTAGTGTCA 
GAGAGGGTAG CCACTCTGGA GCAGAGCTAT GAGGCCCTGT GCGAATTGGC AGCAACTCGA 
AGGGCCCGAC TGGAAGAGTC CCGTCGTCTC TGGAGGTTCC TCTGGGAAGT GGGTGAGGCC 
GAGGCCTGGG TTCGGGAGCA GCAGCACCTC CTGGCCTCAG CTGAGACAGG CCGGGACCTG 
ACTGGTGTCC TCCGCCTGCT CAATAAGCAC ACAGCCCTAC GGGGTGAGAT GAGTGGCCGC 
CTGGGGCCCC TGAAGCTCAC CCTGGAACAA GGTCAGCAGT TAGTTGCCGA GGGCCACCCT 
GGAGCTAACC AAGCCTCAAC CCGTGCAGCA GAGCTCCAGG CCCAGTGGGA GCGACTAGAA 
GCCCTGGCCG AGGAGCGAGC CCAGCGGCTA GCACAGGCTG CCAGCCTCTA CCAGTTCCAG 
GCAGATGCCA ATGACATGGA GGCTTGGTTG GTGGACGCAC TACGCCTGGT ATCTAGCCCT 
GAGGTAGGGC ACGATGAGTT CTCCACGCAG GCCCTGGCCA GGCAGCACAG GGCCCTTGAG 
GAGGAGATCC GAGCCCACCG GCCTACACTG GATGCCTTGA GGGAGCAGGC TGCAGCCCTG 
CCACCTGCAC TGAGCCACAC ACCTGAGGTA CAGGGCAGGG TGCCCACTCT GGAGCAGCAC 
TATGAGGAGC TGCAGGCCCG GGCAGGTGAG CGTGCACGAG CCCTGGAAGC AGCCCTGGCG 
TTCTATACCA TGCTCAGCGA GGCCGGGGCT TGTGGGCTCT GGGTAGAGGA GAAGGAGCAG 
TGGCTCAACG GGCTGGCCCT ACCTGAGCGC CTGGAGGACC TGGAGGTGGT CCAACAGAGG 
TTTGAGACCT TAGAGCCCGA AATGAACGCC CTGGCTGCAC GGGTTACTGC TGTCAGTGAC 
ATAGCTGAGC AGTTGCTGAA GGCCAGTCCA CCAGGCAAGG ACCGCATCAT TGGCACCCAG 
GAGCAGCTCA ACCAAAGGTG GCAGCAGTTC AGGTCCCTGG CAGATGGCAA AAAGGCAGCT 
CTGACATCAG CCCTGAGCAT CCAGAATTAC CACCTAGAGT GCACAGAGAC CCAGGCCTGG 
ATGAGAGAAA AGACCAAGGT CATTGAGTCT ACCCAGGACC TAGGCAATGA TCTAGCTGGT 
GTGCTGGCCC TGCAGCGGAA GCTGGCAGGC ACTGAGAGAG ATCTGGAAGC CATCTCTGCC 
CGGGTGGGTG AGCTGACCCA AGAGGCAAAT GCTTTGGCTG CTGGGCACCC AGCCCAAGCC 
CCTGCCATCA ACACACGGCT TGGAGAGGTT CAAACTGGAT GGGAGGATCT TCGGGCAACC 
ATGAGGCGGA GAGAAGAGTC CCTGGGTGAG GCTCGACGGC TGCAAGATTT CCTGCGCAGC 
TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACACAGACTG CTGTAGCCTC TGAGGAAGGA 
CCAGCCACCC TTCCAGAGGC AGAAGCCCTC TTAGCCCAGC ATGCAGCTCT GCGGGGAGAG 
GTGGAGAGAG CCCAGAGCGA GTACAGCCGC CTCAGGACCT TGGGCGAGGA GGTGACCAGA 
GACCAGGCTG ATCCCCAATG CCTCTTCCTC AGACAGAGGC TGGAAGCCCT TGGAACCGGC 
TGGGAGGAGC TGGGTCGCAT GTGGGAGAGC CGGCAAGGCC GCTTGGCCCA GGCCCATGGC 
TTCCAGGGGT TTTTGAGAGA TGCTCGCCAG GCTGAGGGAG TTCTCAGCAG CCAGGAATAT 
GTTCTATCTC ACACGGAGAT GCCAGGGACA CTGCAGGCGG CGGATGCAGC CATTAAAAAG 
CTGGAAGACT TCATGAGCAC CATGGACGCC AATGGAGAGC GCATCCGTGG ACTCCTGGAG 
GCTGGCCGTC AGCTGGTGTC CAAGGGCAAT ATCCATGCTG AGAAGATCCA AGAGAAGGCA 
GACTCCATCG AGAAGAGGCA CAGAAAGAAC CAGGAGGCCG TGCAGCAGCT TCTAGGCCGC 
CTTCGGGACA ACCGAGAGCA GCAGCACTTC TTGCAAGACT GTCAGGAGCT GAAACTCTGG 
ATTGACGAGA AGATGCTGAC AGCTCAGGAT GTGTCCTATG ATGAGGCACG CAACCTGCAC 
ACCAAGTGGC AAAAACACCA GGCATTCATG GCCGAGCTGG CAGCCAACAA GGACTGGCTG 
GACAAAGTGG ACAAGGAAGG GCGGGAGCTG ACTCTTGAAA AGCCAGAACT CAAAGTCCTA 
GTGTCAGAGA AGCTGGAGGA CCTGCACAGG CGCTGGGATG AACTGGAGAC TACCACCCAA 
GCCAAGGCCC GCAGTCTTTT TGATGCTAAC CGGGCAGAGC TATTTGCCCA AAGCTGTTCT 
GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCAGATGA CTATGGCAAG 
GACCTCACCA GTGTCAACAT TCTGCTAAAG AAGCAACAGA TGCTGGAACG AGAGATGGCT 
GTGAGAGAGA AGGAGGTAGA GGCTATCCAG GCCCAGGCAA AAGCCCTGGC CCAGGAAGAC 
CAAAGTGCAG GAGAGGTGGA AAGGACCTCC AGAGCTGTGG AGGAGAAGTT CAGGGCCTTG 
TGTCAGCCCA TGAAGGACCG CTGCCGGCGC CTGCAAGCCT CCCGAGAGCA GCACCAGTTC 
CACCGGGATG TGGAGGATGA GATACTGTGG GTGACCGAGC GGCTTCCCAT GGCCAGCTCT 
CTGGAGCATG GCAAGGACTT GCCCAGCGTC CAGCTTCTCA TGAAGAAAAA CCAGACTCTG 
CAGAAGGAGA TCCAGGGCCA TGAGCCCCGG ATTGCAGACC TCAAAGAGAG ACAGCGCACT 
CTGGGAACAG CAGCAGCGGG TCCAGAGCTG GCTGAGCTCC AGGAAATGTG GAAACGCCTG 
AGCCATGAGC TGGAGCTTCG GGGTAAACGA CTGGAGGAGG CCCTTCGAGC CCAGCAATTC 
TATCGTGACG CTGCAGAGGC CGAGGCTTGG ATGGGGGAGC AGGAGTTACA TATGATGGGC 
CAGGAAAAGG CCAAGGATGA GCTGAGCGCC CAGGCAGAAG TGAAGAAGCA TCAGGTACTA 
GAACAAGCCC TTGCTGACTA TGCCCAGACC ATCAAACAAC TAGCAGCCAG CAGTCAAGAT 
ATGATTGACC ATGAACATCC AGAGAGCACA AGGTTAACAA TACGCCAAGC CCAGGTGGAC 
AAGCTGTATG CCGGCCTAAA GGAGCTGGCA GGAGAGCGGC GTGAGCGTCT GCAGGAGCAC 
CTCAGGCTGT GCCAGCTCCG CAGAGAGCTG GATGACCTGG AGCAGTGGAT ACAGGAGCGA 
GAAGTCGTGG CAGCCTCCCA TGAACTGGGC CAGGACTATG AGCATGTGAC TATGCTTCGG 
GACAAATTCC GAGAGTTCTC CAGGGACACC AGCACCATTG GCCAAGAGCG TGTAGACAGT 
GCCAATGCCC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACCGTGGCC 
GAGTGGAAGG ACAGTCTCAA TGAGGCCTGG GCTGACCTGC TGGAGCTGCT GGACACAAGA 
GGTCAGGTGC TGGCTGCTGC TTATGAGCTG CAGCGCTTCC TGCATGGGGC CCGCCAAGCC 
CTGGCACGGG TGCAGCACAA GCAGCAGCAG CTTCCAGATG GGACGGGCCG CGACCTCAAT 
GCTGCTGAGG CCCTGCAGCG CCGGCACTGC GCCTATGAGC ACGACATCCA AGCCCTCAGC 
ACTCAGGTCC AGCAGGTTCA GGACGATGGC CTCAGGCTAC AAAAGGCCTA TGCTGGAGAC 
AAGGCTGAGG AAATTGGCCG TCACATGCAG GCAGTGGCTG AGGCGTGGGC CCAGCTCCAG 
GGAAGTTCTG CTGCCCGTCG CCAGCTGTTA CTGGATACCA CAGACAAATT CCGATTCTTC 
AAGGCTGTCC GGGAGTTGAT GCTGTGGATG GATGGGATTA ACCTGCAGAT GGATGCCCAG 
GAGAGGCCCC GGGATGTGTC CTCTGCAGAT TTAGTCATCA AAAACCAACA AGGCATCAAA 
GCAGAGATAG AGGCAAGAGC TGACAGGTTC TCCGCCTGCA TTGACATGGG GCAAGAGCTG 
CTGGCCCGGA ACCACTATGC CGCTGAGGAG ATCTCAGAGA AGCTGTCTCA GCTACAGTCC 
CGGCGCCAGG AGACAGCTGA AAAGTGGCAG GAGAAGATGG ACTGGCTACA GCTTGTTTTG 
GAGGTGCTTG TGTTTGGGAG AGATGCAGGC ATGGCAGAGG CCTGGCTATG CAGTCAGGAG 
CCATTGGTGC GAAGTGCAGA ACTGGGTTGC ACTGTGGATG AAGTAGAGAG CCTCATCAAG 
CGGCATGAAG CCTTCCAGAA GTCAGCAGTG GCCTGGGAGG AGCGTTTCAG TGCCCTGGAG 
AAGCTCACTG CGCTGGAAGA GCGGGAGAAT GAGCAGAAAA GGAAGAGGGA GGAGGAGGAA 
CGAAGGAAAC AGCCCCCTAC TTCAGAGCCC ATGGCTAGTC AACCGGAAGG GAGTCTGGTA 
GATGGCCAGA GAGTTCTTGA CACTGCCTGG GATGGGACCC AGTCAAAATT GCCACCATCC 
ACACAAGCAC CCAGCATTAA TGGGGTCTGC ACGGACACGG AGTCCTCACA GCCCCTGTTG 
GAACAGCAAA GACTTGAACA GAGCAATGTC CCAGAAGGGC CTGGATCTGG CACAGGAGAC 
GAGTCCAGCG GGCCCCGGGG AGAGAGGCAG ACCCTGCCCC GGGGCCCTGC TCCGTCTCCA 
ATGCCCCAGA GCAGATCGTC TGAGTCAGCT CATGTTGCCA CCCTGCCCGC ACGAGGTGCT 
GAGCTCTCTG CTCAGGAACA GATGGAAGGG ACGCTGTGCC GCAAACAGGA GATGGAAGCC 
TTCAATAAGA AAGCTGCCAA CAGGTCCTGG CAGAATGTGT ACTGTGTACT TCGGCGTGGA 
AGCCTCGGCT TTTACAAGGA TGCCAGGGCA GCTAGTGCAG GAGTGCCATA CCATGGAGAA 
GTGCCTGTCA GTCTGGCCAG GGCCCAGGGC AGTGTGGCCT TTGATTATCG GAAACGCAAA 
CATGTCTTCA AGCTGGGCTT GCAGGATGGG AAAGAGTATC TATTCCAGGC CAAGGATGAG 
GCAGAGATGA GCTCATGGCT GAGAGTGGTG AATGCAGCCA TTGCCACTGC GTCCTCGGCC 
TCTGGAGAGC CAGAAGAGCC AGTGGTGCCC AGTGCCAGCC GGGGTCTGAC CAGGGCCATG 
ACCATGCCCC CAGTGTCACA GCCTGAGGGC TCCATCGTGC TTCGCAGCAA GGATGGCAGA 
GAAAGAGAGC GAGAAAAACG ATTCAGCTTC TTTAAGAAGA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_008760093.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758315.1 Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X4, mRNA.

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CloneID	ORa43235
Clone ID Related Accession (Same CDS sequence)	XM_008760093.2
Accession Version	XM_008760093.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACAA ACGGAGCGGG CAGGGTACAT GGAAATGGCC TGAACGGGGC TGGTGAGTTT 
TACTATGAGG CTGTGGAAGG GTCTCAAAAC CCTGGAGGCA TCCTGCTCTC ACCAGCTGCC 
TTCATCAATC CTGCTCAGTA TGCCAGTGTG CTGGAAGGAC GATTCAAACA GCTTCAAGAT 
GAGCGAGAAG CCGTGCAGAA GAAAACCTTC ACCAAGTGGG TGAACTCCCA CCTGGCCCGG 
GTGACATGCC GGGTGGGAGA CCTGTACAGC GACCTGCGGG ACGGGCGCAA CCTCCTGAGG 
CTCCTGGAGG TGCTCTCGGG AGAGACCCTG CCAAAACCCA CCAAGGGCCG GATGCGGATT 
CACTGCCTGG AGAATGTCGA CAAAGCACTG CAGTTCCTGA AGGAGCAGAA GGTGCACCTG 
GAAAACATGG GCTCCCACGA CATTGTGGAT GGGAACCACC GTCTGACCCT TGGGCTAGTG 
TGGACCATCA TCCTCCGATT TCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG 
GAGAAGAAGT CAGCCAAGGA TGCCCTGCTG CTGTGGTGCC AGATGAAGAC TGCAGGGTAT 
CCCAATGTCA ATGTGCACAA CTTTACCACC AGTTGGAGAG ATGGGCTGGC CTTTAATGCC 
ATTGTGCACA AACACCGGCC AGACCTGTTG GATTTTGAGT CCCTGAAGAA GTGTAACGCA 
CACTACAATC TGCAGAATGC TTTCAATCTG GCTGAAAAGG AACTTGGCCT GACGAAGCTC 
CTGGATCCTG AAGATGTGAA CGTAGACCAA CCCGATGAGA AGTCCATCAT CACCTACGTG 
GCCACTTACT ACCACTACTT CTCGAAGATG AAGGCCCTGG CTGTGGAAGG CAAAAGGATT 
GGCAAGGTCC TGGACCATGC CATGGAGGCA GAACACCTGG TAGAGAAATA TGAGTCCCTG 
GCCTCTGAAC TGCTGCAGTG GATCGAGCAA ACGATTGTGA CCCTCAATGA CCGACAGCTG 
GCCAACTCCC TGAGTGGCGT CCAGAACCAG CTGCAGTCTT TCAATTCCTA CCGCACGGTG 
GAGAAGCCAC CCAAGTTCAC AGAGAAAGGG AACTTGGAGG TGTTGCTCTT CACCATCCAG 
AGTAAGCTGC GGGCCAACAA CCAGAAAGTC TACACACCAC GCGAAGGCCG GCTCATCTCG 
GACATCAACA AGGCCTGGGA GCGGCTAGAG AAAGCCGAAC ATGAGCGAGA GCTGGCCCTG 
CGCACGGAGC TGATCCGCCA GGAGAAGCTG GAGCAACTGG CTGCTCGCTT CGACCGCAAG 
GCTGCCATGC GGGAGACCTG GCTCAGTGAG AACCAGCGCC TCGTCTCCCA GGACAACTTT 
GGCCTGGAGC TGGCAGCAGT GGAGGCAGCA GTGCGGAAGC ATGAAGCCAT TGAGACAGAC 
ATTGTGGCCT ACAGCGGCCG GGTGCAAGCG GTGGACGCCG TAGCCGCAGA ACTGGCCGCT 
GAGCACTACC ATGACATTAA GCGCATTGCG GCGCGGCAGA ACAACGTGGC CCGGCTCTGG 
GACTTCTTAC GAGAGATGGT GGCCGCCCGC CGTGAGCGGC TCCTTCTCAA CCTGGAGCTG 
CAGAAGGTGT TTCAGGACCT GCTCTACCTC ATGGACTGGA TGGCAGAGAT GAAGGGCCGG 
CTGCAGTCTC AGGACCTAGG CAAGCATCTG GCTGGAGTGG AAGATCTACT GCAACTACAC 
GAACTGGTGG AGGCGGACAT TGCAGTTCAG GCTGAGAGGG TGCGAGCGGT CAGCGCCTCT 
GCCCTGCGCT TCTGCGACCC CGGGAAAGAG TATAGACCAT GTGACCCACA GCTAGTGTCA 
GAGAGGGTAG CCACTCTGGA GCAGAGCTAT GAGGCCCTGT GCGAATTGGC AGCAACTCGA 
AGGGCCCGAC TGGAAGAGTC CCGTCGTCTC TGGAGGTTCC TCTGGGAAGT GGGTGAGGCC 
GAGGCCTGGG TTCGGGAGCA GCAGCACCTC CTGGCCTCAG CTGAGACAGG CCGGGACCTG 
ACTGGTGTCC TCCGCCTGCT CAATAAGCAC ACAGCCCTAC GGGGTGAGAT GAGTGGCCGC 
CTGGGGCCCC TGAAGCTCAC CCTGGAACAA GGTCAGCAGT TAGTTGCCGA GGGCCACCCT 
GGAGCTAACC AAGCCTCAAC CCGTGCAGCA GAGCTCCAGG CCCAGTGGGA GCGACTAGAA 
GCCCTGGCCG AGGAGCGAGC CCAGCGGCTA GCACAGGCTG CCAGCCTCTA CCAGTTCCAG 
GCAGATGCCA ATGACATGGA GGCTTGGTTG GTGGACGCAC TACGCCTGGT ATCTAGCCCT 
GAGGTAGGGC ACGATGAGTT CTCCACGCAG GCCCTGGCCA GGCAGCACAG GGCCCTTGAG 
GAGGAGATCC GAGCCCACCG GCCTACACTG GATGCCTTGA GGGAGCAGGC TGCAGCCCTG 
CCACCTGCAC TGAGCCACAC ACCTGAGGTA CAGGGCAGGG TGCCCACTCT GGAGCAGCAC 
TATGAGGAGC TGCAGGCCCG GGCAGGTGAG CGTGCACGAG CCCTGGAAGC AGCCCTGGCG 
TTCTATACCA TGCTCAGCGA GGCCGGGGCT TGTGGGCTCT GGGTAGAGGA GAAGGAGCAG 
TGGCTCAACG GGCTGGCCCT ACCTGAGCGC CTGGAGGACC TGGAGGTGGT CCAACAGAGG 
TTTGAGACCT TAGAGCCCGA AATGAACGCC CTGGCTGCAC GGGTTACTGC TGTCAGTGAC 
ATAGCTGAGC AGTTGCTGAA GGCCAGTCCA CCAGGCAAGG ACCGCATCAT TGGCACCCAG 
GAGCAGCTCA ACCAAAGGTG GCAGCAGTTC AGGTCCCTGG CAGATGGCAA AAAGGCAGCT 
CTGACATCAG CCCTGAGCAT CCAGAATTAC CACCTAGAGT GCACAGAGAC CCAGGCCTGG 
ATGAGAGAAA AGACCAAGGT CATTGAGTCT ACCCAGGACC TAGGCAATGA TCTAGCTGGT 
GTGCTGGCCC TGCAGCGGAA GCTGGCAGGC ACTGAGAGAG ATCTGGAAGC CATCTCTGCC 
CGGGTGGGTG AGCTGACCCA AGAGGCAAAT GCTTTGGCTG CTGGGCACCC AGCCCAAGCC 
CCTGCCATCA ACACACGGCT TGGAGAGGTT CAAACTGGAT GGGAGGATCT TCGGGCAACC 
ATGAGGCGGA GAGAAGAGTC CCTGGGTGAG GCTCGACGGC TGCAAGATTT CCTGCGCAGC 
TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACACAGACTG CTGTAGCCTC TGAGGAAGGA 
CCAGCCACCC TTCCAGAGGC AGAAGCCCTC TTAGCCCAGC ATGCAGCTCT GCGGGGAGAG 
GTGGAGAGAG CCCAGAGCGA GTACAGCCGC CTCAGGACCT TGGGCGAGGA GGTGACCAGA 
GACCAGGCTG ATCCCCAATG CCTCTTCCTC AGACAGAGGC TGGAAGCCCT TGGAACCGGC 
TGGGAGGAGC TGGGTCGCAT GTGGGAGAGC CGGCAAGGCC GCTTGGCCCA GGCCCATGGC 
TTCCAGGGGT TTTTGAGAGA TGCTCGCCAG GCTGAGGGAG TTCTCAGCAG CCAGGAATAT 
GTTCTATCTC ACACGGAGAT GCCAGGGACA CTGCAGGCGG CGGATGCAGC CATTAAAAAG 
CTGGAAGACT TCATGAGCAC CATGGACGCC AATGGAGAGC GCATCCGTGG ACTCCTGGAG 
GCTGGCCGTC AGCTGGTGTC CAAGGGCAAT ATCCATGCTG AGAAGATCCA AGAGAAGGCA 
GACTCCATCG AGAAGAGGCA CAGAAAGAAC CAGGAGGCCG TGCAGCAGCT TCTAGGCCGC 
CTTCGGGACA ACCGAGAGCA GCAGCACTTC TTGCAAGACT GTCAGGAGCT GAAACTCTGG 
ATTGACGAGA AGATGCTGAC AGCTCAGGAT GTGTCCTATG ATGAGGCACG CAACCTGCAC 
ACCAAGTGGC AAAAACACCA GGCATTCATG GCCGAGCTGG CAGCCAACAA GGACTGGCTG 
GACAAAGTGG ACAAGGAAGG GCGGGAGCTG ACTCTTGAAA AGCCAGAACT CAAAGTCCTA 
GTGTCAGAGA AGCTGGAGGA CCTGCACAGG CGCTGGGATG AACTGGAGAC TACCACCCAA 
GCCAAGGCCC GCAGTCTTTT TGATGCTAAC CGGGCAGAGC TATTTGCCCA AAGCTGTTCT 
GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCAGATGA CTATGGCAAG 
GACCTCACCA GTGTCAACAT TCTGCTAAAG AAGCAACAGA TGCTGGAACG AGAGATGGCT 
GTGAGAGAGA AGGAGGTAGA GGCTATCCAG GCCCAGGCAA AAGCCCTGGC CCAGGAAGAC 
CAAAGTGCAG GAGAGGTGGA AAGGACCTCC AGAGCTGTGG AGGAGAAGTT CAGGGCCTTG 
TGTCAGCCCA TGAAGGACCG CTGCCGGCGC CTGCAAGCCT CCCGAGAGCA GCACCAGTTC 
CACCGGGATG TGGAGGATGA GATACTGTGG GTGACCGAGC GGCTTCCCAT GGCCAGCTCT 
CTGGAGCATG GCAAGGACTT GCCCAGCGTC CAGCTTCTCA TGAAGAAAAA CCAGACTCTG 
CAGAAGGAGA TCCAGGGCCA TGAGCCCCGG ATTGCAGACC TCAAAGAGAG ACAGCGCACT 
CTGGGAACAG CAGCAGCGGG TCCAGAGCTG GCTGAGCTCC AGGAAATGTG GAAACGCCTG 
AGCCATGAGC TGGAGCTTCG GGGTAAACGA CTGGAGGAGG CCCTTCGAGC CCAGCAATTC 
TATCGTGACG CTGCAGAGGC CGAGGCTTGG ATGGGGGAGC AGGAGTTACA TATGATGGGC 
CAGGAAAAGG CCAAGGATGA GCTGAGCGCC CAGGCAGAAG TGAAGAAGCA TCAGGTACTA 
GAACAAGCCC TTGCTGACTA TGCCCAGACC ATCAAACAAC TAGCAGCCAG CAGTCAAGAT 
ATGATTGACC ATGAACATCC AGAGAGCACA AGGTTAACAA TACGCCAAGC CCAGGTGGAC 
AAGCTGTATG CCGGCCTAAA GGAGCTGGCA GGAGAGCGGC GTGAGCGTCT GCAGGAGCAC 
CTCAGGCTGT GCCAGCTCCG CAGAGAGCTG GATGACCTGG AGCAGTGGAT ACAGGAGCGA 
GAAGTCGTGG CAGCCTCCCA TGAACTGGGC CAGGACTATG AGCATGTGAC TATGCTTCGG 
GACAAATTCC GAGAGTTCTC CAGGGACACC AGCACCATTG GCCAAGAGCG TGTAGACAGT 
GCCAATGCCC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACCGTGGCC 
GAGTGGAAGG ACAGTCTCAA TGAGGCCTGG GCTGACCTGC TGGAGCTGCT GGACACAAGA 
GGTCAGGTGC TGGCTGCTGC TTATGAGCTG CAGCGCTTCC TGCATGGGGC CCGCCAAGCC 
CTGGCACGGG TGCAGCACAA GCAGCAGCAG CTTCCAGATG GGACGGGCCG CGACCTCAAT 
GCTGCTGAGG CCCTGCAGCG CCGGCACTGC GCCTATGAGC ACGACATCCA AGCCCTCAGC 
ACTCAGGTCC AGCAGGTTCA GGACGATGGC CTCAGGCTAC AAAAGGCCTA TGCTGGAGAC 
AAGGCTGAGG AAATTGGCCG TCACATGCAG GCAGTGGCTG AGGCGTGGGC CCAGCTCCAG 
GGAAGTTCTG CTGCCCGTCG CCAGCTGTTA CTGGATACCA CAGACAAATT CCGATTCTTC 
AAGGCTGTCC GGGAGTTGAT GCTGTGGATG GATGGGATTA ACCTGCAGAT GGATGCCCAG 
GAGAGGCCCC GGGATGTGTC CTCTGCAGAT TTAGTCATCA AAAACCAACA AGGCATCAAA 
GCAGAGATAG AGGCAAGAGC TGACAGGTTC TCCGCCTGCA TTGACATGGG GCAAGAGCTG 
CTGGCCCGGA ACCACTATGC CGCTGAGGAG ATCTCAGAGA AGCTGTCTCA GCTACAGTCC 
CGGCGCCAGG AGACAGCTGA AAAGTGGCAG GAGAAGATGG ACTGGCTACA GCTTGTTTTG 
GAGGTGCTTG TGTTTGGGAG AGATGCAGGC ATGGCAGAGG CCTGGCTATG CAGTCAGGAG 
CCATTGGTGC GAAGTGCAGA ACTGGGTTGC ACTGTGGATG AAGTAGAGAG CCTCATCAAG 
CGGCATGAAG CCTTCCAGAA GTCAGCAGTG GCCTGGGAGG AGCGTTTCAG TGCCCTGGAG 
AAGCTCACTG CGCTGGAAGA GCGGGAGAAT GAGCAGAAAA GGAAGAGGGA GGAGGAGGAA 
CGAAGGAAAC AGCCCCCTAC TTCAGAGCCC ATGGCTAGTC AACCGGAAGG GAGTCTGGTA 
GATGGCCAGA GAGTTCTTGA CACTGCCTGG GATGGGACCC AGTCAAAATT GCCACCATCC 
ACACAAGCAC CCAGCATTAA TGGGGTCTGC ACGGACACGG AGTCCTCACA GCCCCTGTTG 
GAACAGCAAA GACTTGAACA GAGCAATGTC CCAGAAGGGC CTGGATCTGG CACAGGAGAC 
GAGTCCAGCG GGCCCCGGGG AGAGAGGCAG ACCCTGCCCC GGGGCCCTGC TCCGTCTCCA 
ATGCCCCAGA GCAGATCGTC TGAGTCAGCT CATGTTGCCA CCCTGCCCGC ACGAGGTGCT 
GAGCTCTCTG CTCAGGAACA GATGGAAGGG ACGCTGTGCC GCAAACAGGA GATGGAAGCC 
TTCAATAAGA AAGCTGCCAA CAGGTCCTGG CAGAATGTGT ACTGTGTACT TCGGCGTGGA 
AGCCTCGGCT TTTACAAGGA TGCCAGGGCA GCTAGTGCAG GAGTGCCATA CCATGGAGAA 
GTGCCTGTCA GTCTGGCCAG GGCCCAGGGC AGTGTGGCCT TTGATTATCG GAAACGCAAA 
CATGTCTTCA AGCTGGGCTT GCAGGATGGG AAAGAGTATC TATTCCAGGC CAAGGATGAG 
AGTGGCTTTA AACTCATACC AATCCTCCTA CCTAAGCCTT CTGAGTCTGG GCTCACAGGT 
ATATGCCACC ATGACAAACT GATGCTCCCC ATCCTAATGG GATCCTTCTC TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758315.1
CDS	226..7302
Translation	MSTNGAGRVHGNGLNGAGEFYYEAVEGSQNPGGILLSPAAFINPAQYASVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLREMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVSDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQDLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVLVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAKALAQEDQSAGEVERTSRAVEEKFRALCQPMKDRCRRLQASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYAGLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGLRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSACIDMGQELLARNHYAAEEISEKLSQLQSRRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENEQKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVLDTAWDGTQSKLPPSTQAPSINGVCTDTESSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSESAHVATLPARGAELSAQEQMEGTLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDESGFKLIPILLPKPSESGLTGICHHDKLMLPILMGSFSF

Target ORF information:

RefSeq Version	XM_008760093.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760093.2

ORF Insert Sequence:

ATGAGTACAA ACGGAGCGGG CAGGGTACAT GGAAATGGCC TGAACGGGGC TGGTGAGTTT 
TACTATGAGG CTGTGGAAGG GTCTCAAAAC CCTGGAGGCA TCCTGCTCTC ACCAGCTGCC 
TTCATCAATC CTGCTCAGTA TGCCAGTGTG CTGGAAGGAC GATTCAAACA GCTTCAAGAT 
GAGCGAGAAG CCGTGCAGAA GAAAACCTTC ACCAAGTGGG TGAACTCCCA CCTGGCCCGG 
GTGACATGCC GGGTGGGAGA CCTGTACAGC GACCTGCGGG ACGGGCGCAA CCTCCTGAGG 
CTCCTGGAGG TGCTCTCGGG AGAGACCCTG CCAAAACCCA CCAAGGGCCG GATGCGGATT 
CACTGCCTGG AGAATGTCGA CAAAGCACTG CAGTTCCTGA AGGAGCAGAA GGTGCACCTG 
GAAAACATGG GCTCCCACGA CATTGTGGAT GGGAACCACC GTCTGACCCT TGGGCTAGTG 
TGGACCATCA TCCTCCGATT TCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG 
GAGAAGAAGT CAGCCAAGGA TGCCCTGCTG CTGTGGTGCC AGATGAAGAC TGCAGGGTAT 
CCCAATGTCA ATGTGCACAA CTTTACCACC AGTTGGAGAG ATGGGCTGGC CTTTAATGCC 
ATTGTGCACA AACACCGGCC AGACCTGTTG GATTTTGAGT CCCTGAAGAA GTGTAACGCA 
CACTACAATC TGCAGAATGC TTTCAATCTG GCTGAAAAGG AACTTGGCCT GACGAAGCTC 
CTGGATCCTG AAGATGTGAA CGTAGACCAA CCCGATGAGA AGTCCATCAT CACCTACGTG 
GCCACTTACT ACCACTACTT CTCGAAGATG AAGGCCCTGG CTGTGGAAGG CAAAAGGATT 
GGCAAGGTCC TGGACCATGC CATGGAGGCA GAACACCTGG TAGAGAAATA TGAGTCCCTG 
GCCTCTGAAC TGCTGCAGTG GATCGAGCAA ACGATTGTGA CCCTCAATGA CCGACAGCTG 
GCCAACTCCC TGAGTGGCGT CCAGAACCAG CTGCAGTCTT TCAATTCCTA CCGCACGGTG 
GAGAAGCCAC CCAAGTTCAC AGAGAAAGGG AACTTGGAGG TGTTGCTCTT CACCATCCAG 
AGTAAGCTGC GGGCCAACAA CCAGAAAGTC TACACACCAC GCGAAGGCCG GCTCATCTCG 
GACATCAACA AGGCCTGGGA GCGGCTAGAG AAAGCCGAAC ATGAGCGAGA GCTGGCCCTG 
CGCACGGAGC TGATCCGCCA GGAGAAGCTG GAGCAACTGG CTGCTCGCTT CGACCGCAAG 
GCTGCCATGC GGGAGACCTG GCTCAGTGAG AACCAGCGCC TCGTCTCCCA GGACAACTTT 
GGCCTGGAGC TGGCAGCAGT GGAGGCAGCA GTGCGGAAGC ATGAAGCCAT TGAGACAGAC 
ATTGTGGCCT ACAGCGGCCG GGTGCAAGCG GTGGACGCCG TAGCCGCAGA ACTGGCCGCT 
GAGCACTACC ATGACATTAA GCGCATTGCG GCGCGGCAGA ACAACGTGGC CCGGCTCTGG 
GACTTCTTAC GAGAGATGGT GGCCGCCCGC CGTGAGCGGC TCCTTCTCAA CCTGGAGCTG 
CAGAAGGTGT TTCAGGACCT GCTCTACCTC ATGGACTGGA TGGCAGAGAT GAAGGGCCGG 
CTGCAGTCTC AGGACCTAGG CAAGCATCTG GCTGGAGTGG AAGATCTACT GCAACTACAC 
GAACTGGTGG AGGCGGACAT TGCAGTTCAG GCTGAGAGGG TGCGAGCGGT CAGCGCCTCT 
GCCCTGCGCT TCTGCGACCC CGGGAAAGAG TATAGACCAT GTGACCCACA GCTAGTGTCA 
GAGAGGGTAG CCACTCTGGA GCAGAGCTAT GAGGCCCTGT GCGAATTGGC AGCAACTCGA 
AGGGCCCGAC TGGAAGAGTC CCGTCGTCTC TGGAGGTTCC TCTGGGAAGT GGGTGAGGCC 
GAGGCCTGGG TTCGGGAGCA GCAGCACCTC CTGGCCTCAG CTGAGACAGG CCGGGACCTG 
ACTGGTGTCC TCCGCCTGCT CAATAAGCAC ACAGCCCTAC GGGGTGAGAT GAGTGGCCGC 
CTGGGGCCCC TGAAGCTCAC CCTGGAACAA GGTCAGCAGT TAGTTGCCGA GGGCCACCCT 
GGAGCTAACC AAGCCTCAAC CCGTGCAGCA GAGCTCCAGG CCCAGTGGGA GCGACTAGAA 
GCCCTGGCCG AGGAGCGAGC CCAGCGGCTA GCACAGGCTG CCAGCCTCTA CCAGTTCCAG 
GCAGATGCCA ATGACATGGA GGCTTGGTTG GTGGACGCAC TACGCCTGGT ATCTAGCCCT 
GAGGTAGGGC ACGATGAGTT CTCCACGCAG GCCCTGGCCA GGCAGCACAG GGCCCTTGAG 
GAGGAGATCC GAGCCCACCG GCCTACACTG GATGCCTTGA GGGAGCAGGC TGCAGCCCTG 
CCACCTGCAC TGAGCCACAC ACCTGAGGTA CAGGGCAGGG TGCCCACTCT GGAGCAGCAC 
TATGAGGAGC TGCAGGCCCG GGCAGGTGAG CGTGCACGAG CCCTGGAAGC AGCCCTGGCG 
TTCTATACCA TGCTCAGCGA GGCCGGGGCT TGTGGGCTCT GGGTAGAGGA GAAGGAGCAG 
TGGCTCAACG GGCTGGCCCT ACCTGAGCGC CTGGAGGACC TGGAGGTGGT CCAACAGAGG 
TTTGAGACCT TAGAGCCCGA AATGAACGCC CTGGCTGCAC GGGTTACTGC TGTCAGTGAC 
ATAGCTGAGC AGTTGCTGAA GGCCAGTCCA CCAGGCAAGG ACCGCATCAT TGGCACCCAG 
GAGCAGCTCA ACCAAAGGTG GCAGCAGTTC AGGTCCCTGG CAGATGGCAA AAAGGCAGCT 
CTGACATCAG CCCTGAGCAT CCAGAATTAC CACCTAGAGT GCACAGAGAC CCAGGCCTGG 
ATGAGAGAAA AGACCAAGGT CATTGAGTCT ACCCAGGACC TAGGCAATGA TCTAGCTGGT 
GTGCTGGCCC TGCAGCGGAA GCTGGCAGGC ACTGAGAGAG ATCTGGAAGC CATCTCTGCC 
CGGGTGGGTG AGCTGACCCA AGAGGCAAAT GCTTTGGCTG CTGGGCACCC AGCCCAAGCC 
CCTGCCATCA ACACACGGCT TGGAGAGGTT CAAACTGGAT GGGAGGATCT TCGGGCAACC 
ATGAGGCGGA GAGAAGAGTC CCTGGGTGAG GCTCGACGGC TGCAAGATTT CCTGCGCAGC 
TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACACAGACTG CTGTAGCCTC TGAGGAAGGA 
CCAGCCACCC TTCCAGAGGC AGAAGCCCTC TTAGCCCAGC ATGCAGCTCT GCGGGGAGAG 
GTGGAGAGAG CCCAGAGCGA GTACAGCCGC CTCAGGACCT TGGGCGAGGA GGTGACCAGA 
GACCAGGCTG ATCCCCAATG CCTCTTCCTC AGACAGAGGC TGGAAGCCCT TGGAACCGGC 
TGGGAGGAGC TGGGTCGCAT GTGGGAGAGC CGGCAAGGCC GCTTGGCCCA GGCCCATGGC 
TTCCAGGGGT TTTTGAGAGA TGCTCGCCAG GCTGAGGGAG TTCTCAGCAG CCAGGAATAT 
GTTCTATCTC ACACGGAGAT GCCAGGGACA CTGCAGGCGG CGGATGCAGC CATTAAAAAG 
CTGGAAGACT TCATGAGCAC CATGGACGCC AATGGAGAGC GCATCCGTGG ACTCCTGGAG 
GCTGGCCGTC AGCTGGTGTC CAAGGGCAAT ATCCATGCTG AGAAGATCCA AGAGAAGGCA 
GACTCCATCG AGAAGAGGCA CAGAAAGAAC CAGGAGGCCG TGCAGCAGCT TCTAGGCCGC 
CTTCGGGACA ACCGAGAGCA GCAGCACTTC TTGCAAGACT GTCAGGAGCT GAAACTCTGG 
ATTGACGAGA AGATGCTGAC AGCTCAGGAT GTGTCCTATG ATGAGGCACG CAACCTGCAC 
ACCAAGTGGC AAAAACACCA GGCATTCATG GCCGAGCTGG CAGCCAACAA GGACTGGCTG 
GACAAAGTGG ACAAGGAAGG GCGGGAGCTG ACTCTTGAAA AGCCAGAACT CAAAGTCCTA 
GTGTCAGAGA AGCTGGAGGA CCTGCACAGG CGCTGGGATG AACTGGAGAC TACCACCCAA 
GCCAAGGCCC GCAGTCTTTT TGATGCTAAC CGGGCAGAGC TATTTGCCCA AAGCTGTTCT 
GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCAGATGA CTATGGCAAG 
GACCTCACCA GTGTCAACAT TCTGCTAAAG AAGCAACAGA TGCTGGAACG AGAGATGGCT 
GTGAGAGAGA AGGAGGTAGA GGCTATCCAG GCCCAGGCAA AAGCCCTGGC CCAGGAAGAC 
CAAAGTGCAG GAGAGGTGGA AAGGACCTCC AGAGCTGTGG AGGAGAAGTT CAGGGCCTTG 
TGTCAGCCCA TGAAGGACCG CTGCCGGCGC CTGCAAGCCT CCCGAGAGCA GCACCAGTTC 
CACCGGGATG TGGAGGATGA GATACTGTGG GTGACCGAGC GGCTTCCCAT GGCCAGCTCT 
CTGGAGCATG GCAAGGACTT GCCCAGCGTC CAGCTTCTCA TGAAGAAAAA CCAGACTCTG 
CAGAAGGAGA TCCAGGGCCA TGAGCCCCGG ATTGCAGACC TCAAAGAGAG ACAGCGCACT 
CTGGGAACAG CAGCAGCGGG TCCAGAGCTG GCTGAGCTCC AGGAAATGTG GAAACGCCTG 
AGCCATGAGC TGGAGCTTCG GGGTAAACGA CTGGAGGAGG CCCTTCGAGC CCAGCAATTC 
TATCGTGACG CTGCAGAGGC CGAGGCTTGG ATGGGGGAGC AGGAGTTACA TATGATGGGC 
CAGGAAAAGG CCAAGGATGA GCTGAGCGCC CAGGCAGAAG TGAAGAAGCA TCAGGTACTA 
GAACAAGCCC TTGCTGACTA TGCCCAGACC ATCAAACAAC TAGCAGCCAG CAGTCAAGAT 
ATGATTGACC ATGAACATCC AGAGAGCACA AGGTTAACAA TACGCCAAGC CCAGGTGGAC 
AAGCTGTATG CCGGCCTAAA GGAGCTGGCA GGAGAGCGGC GTGAGCGTCT GCAGGAGCAC 
CTCAGGCTGT GCCAGCTCCG CAGAGAGCTG GATGACCTGG AGCAGTGGAT ACAGGAGCGA 
GAAGTCGTGG CAGCCTCCCA TGAACTGGGC CAGGACTATG AGCATGTGAC TATGCTTCGG 
GACAAATTCC GAGAGTTCTC CAGGGACACC AGCACCATTG GCCAAGAGCG TGTAGACAGT 
GCCAATGCCC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACCGTGGCC 
GAGTGGAAGG ACAGTCTCAA TGAGGCCTGG GCTGACCTGC TGGAGCTGCT GGACACAAGA 
GGTCAGGTGC TGGCTGCTGC TTATGAGCTG CAGCGCTTCC TGCATGGGGC CCGCCAAGCC 
CTGGCACGGG TGCAGCACAA GCAGCAGCAG CTTCCAGATG GGACGGGCCG CGACCTCAAT 
GCTGCTGAGG CCCTGCAGCG CCGGCACTGC GCCTATGAGC ACGACATCCA AGCCCTCAGC 
ACTCAGGTCC AGCAGGTTCA GGACGATGGC CTCAGGCTAC AAAAGGCCTA TGCTGGAGAC 
AAGGCTGAGG AAATTGGCCG TCACATGCAG GCAGTGGCTG AGGCGTGGGC CCAGCTCCAG 
GGAAGTTCTG CTGCCCGTCG CCAGCTGTTA CTGGATACCA CAGACAAATT CCGATTCTTC 
AAGGCTGTCC GGGAGTTGAT GCTGTGGATG GATGGGATTA ACCTGCAGAT GGATGCCCAG 
GAGAGGCCCC GGGATGTGTC CTCTGCAGAT TTAGTCATCA AAAACCAACA AGGCATCAAA 
GCAGAGATAG AGGCAAGAGC TGACAGGTTC TCCGCCTGCA TTGACATGGG GCAAGAGCTG 
CTGGCCCGGA ACCACTATGC CGCTGAGGAG ATCTCAGAGA AGCTGTCTCA GCTACAGTCC 
CGGCGCCAGG AGACAGCTGA AAAGTGGCAG GAGAAGATGG ACTGGCTACA GCTTGTTTTG 
GAGGTGCTTG TGTTTGGGAG AGATGCAGGC ATGGCAGAGG CCTGGCTATG CAGTCAGGAG 
CCATTGGTGC GAAGTGCAGA ACTGGGTTGC ACTGTGGATG AAGTAGAGAG CCTCATCAAG 
CGGCATGAAG CCTTCCAGAA GTCAGCAGTG GCCTGGGAGG AGCGTTTCAG TGCCCTGGAG 
AAGCTCACTG CGCTGGAAGA GCGGGAGAAT GAGCAGAAAA GGAAGAGGGA GGAGGAGGAA 
CGAAGGAAAC AGCCCCCTAC TTCAGAGCCC ATGGCTAGTC AACCGGAAGG GAGTCTGGTA 
GATGGCCAGA GAGTTCTTGA CACTGCCTGG GATGGGACCC AGTCAAAATT GCCACCATCC 
ACACAAGCAC CCAGCATTAA TGGGGTCTGC ACGGACACGG AGTCCTCACA GCCCCTGTTG 
GAACAGCAAA GACTTGAACA GAGCAATGTC CCAGAAGGGC CTGGATCTGG CACAGGAGAC 
GAGTCCAGCG GGCCCCGGGG AGAGAGGCAG ACCCTGCCCC GGGGCCCTGC TCCGTCTCCA 
ATGCCCCAGA GCAGATCGTC TGAGTCAGCT CATGTTGCCA CCCTGCCCGC ACGAGGTGCT 
GAGCTCTCTG CTCAGGAACA GATGGAAGGG ACGCTGTGCC GCAAACAGGA GATGGAAGCC 
TTCAATAAGA AAGCTGCCAA CAGGTCCTGG CAGAATGTGT ACTGTGTACT TCGGCGTGGA 
AGCCTCGGCT TTTACAAGGA TGCCAGGGCA GCTAGTGCAG GAGTGCCATA CCATGGAGAA 
GTGCCTGTCA GTCTGGCCAG GGCCCAGGGC AGTGTGGCCT TTGATTATCG GAAACGCAAA 
CATGTCTTCA AGCTGGGCTT GCAGGATGGG AAAGAGTATC TATTCCAGGC CAAGGATGAG 
AGTGGCTTTA AACTCATACC AATCCTCCTA CCTAAGCCTT CTGAGTCTGG GCTCACAGGT 
ATATGCCACC ATGACAAACT GATGCTCCCC ATCCTAATGG GATCCTTCTC TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?III Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(27)6442-6459(2017 Jul)
Efimova N,Korobova F,Stankewich MC,Moberly AH,Stolz DB,Wang J,Kashina A,Ma M,Svitkina T

Degradation of ?II-Spectrin Protein by Calpain-2 and Caspase-3 Under Neurotoxic and Traumatic Brain Injury Conditions.
Molecular neurobiology52(1)696-709(2015 Aug)
Kobeissy FH,Liu MC,Yang Z,Zhang Z,Zheng W,Glushakova O,Mondello S,Anagli J,Hayes RL,Wang KK

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

?III spectrin regulates the structural integrity and the secretory protein transport of the Golgi complex.
The Journal of biological chemistry288(4)2157-66(2013 Jan)
Salcedo-Sicilia L,Granell S,Jovic M,Sicart A,Mato E,Johannes L,Balla T,Egea G

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

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Sptbn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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