Vtn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vtn
Entrez Gene ID	29169
Full Name	vitronectin
Synonyms	Aa1018,Vn
General protein information	Preferred Names vitronectin Names vitronectin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q25
Summary	may act as a cofactor for plasminogen activator inhibitor 1 (PAI-1) inhibition of thrombin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019156.2	NP_062029.2	vitronectin precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	VTN	XP_854040.1
Gallus gallus (chicken)	VTN	NP_990392.1
Danio rerio (zebrafish)	vtna	NP_001018508.1
Xenopus tropicalis (tropical clawed frog)	vtn	NP_001184007.1
Pan troglodytes (chimpanzee)	VTN	XP_001146856.1
	VTN	XP_001106884.1
Mus musculus (house mouse)	Vtn	NP_035837.1
Danio rerio (zebrafish)	vtnb	NP_001132933.1
Homo sapiens (human)	VTN	NP_000629.3
Bos taurus (cattle)	VTN	NP_001030222.1
Rattus norvegicus (Norway rat)	Vtn	NP_062029.2

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Sialylation of vitronectin regulates stress fiber formation and cell spreading of dermal fibroblasts via a heparin-binding site.
Miyamoto Y, Tanabe M, Date K, Sakuda K, Sano K, Ogawa H
Glycoconjugate journal33(2)227-36(2016 Apr)

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Brafman DA, Phung C, Kumar N, Willert K
Cell death and differentiation20(3)369-81(2013 Mar)

Purkinje cell maturation participates in the control of oligodendrocyte differentiation: role of sonic hedgehog and vitronectin.
Bouslama-Oueghlani L, Wehrl? R, Doulazmi M, Chen XR, Jaudon F, Lemaigre-Dubreuil Y, Rivals I, Sotelo C, Dusart I
PloS one7(11)e49015(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that sialylation of VN glycans regulates stress fiber formation and cell spreading of dermal fibroblast cells via a heparin binding site.
Title: Sialylation of vitronectin regulates stress fiber formation and cell spreading of dermal fibroblasts via a heparin-binding site.

vitronectin has a role in survival signals of hepatic stellate cells in liver regeneration, which is regulated by glycosylation and sialylation changes
Title: Survival signals of hepatic stellate cells in liver regeneration are regulated by glycosylation changes in rat vitronectin, especially decreased sialylation.

VN is a key determinant of the antiproliferative effect of PAI-1 overexpression.
Title: Recombinant plasminogen activator inhibitor-1 inhibits intimal hyperplasia.

glycan alterations during tissue remodeling induce increased multimerization state to enhance the biological activity of VN
Title: Changes in glycosylation of vitronectin modulate multimerization and collagen binding during liver regeneration.

Lens cell engagement with vitronectin or fibronectin, but not laminin, substratum has potent epithelial mesenchymal transition promoting effect, and Smad 2/3 signaling is involved.
Title: Vitronectin is present in epithelial cells of the intact lens and promotes epithelial mesenchymal transition in lens epithelial explants.

The following Vtn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vtn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03878	NM_019156.2 Latest version!	Rattus norvegicus vitronectin (Vtn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vtn ( NM_019156.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062029.2 Rattus norvegicus vitronectin (Vtn), mRNA.

Price & Availability↑

CloneID	ORa03878
Clone ID Related Accession (Same CDS sequence)	NM_019156.2
Accession Version	NM_019156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-07
Organism	Rattus norvegicus(Norway rat)
Product	vitronectin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105821.1. On Dec 11, 2007 this sequence version replaced NM_019156.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105821.1, FQ209682.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCTC TGAGGCCCTT TTTCATACTA GCCCTGCTGG CATTGGTTTC TCTGGCTGAC 
CAAGAGTCAT GCAAGGGCCG CTGCACTCAG GGTTTCATGG CCAGCAAGAA GTGTCAATGT 
GATGAGCTTT GCACCTACTA TCAGAGCTGC TGTGTTGACT ACATGGAGCA GTGCAAGCCC 
CAAGTGACCC GGGGGGATGT GTTTACCATG CCAGAAGATG AGTATTGGAG CTATGACTAC 
CCGGAGGAAA CCAAGAACAG TACCAGCACT GGCGTGCAAT CCGAGAACAC CTCTCTACAC 
TTCAACCTAA AGCCTCGAGC CGAAGAGACT ATAAAGCCAA CAACTCCTGA TCCTCAGGAA 
CAGTCAAACA CCCAAGAACC TGAAGTGGGG CAACAGGGAG TGGCCCCAAG GCCTGACACC 
ACTGATGAAG GAACCTCTGA GTTTCCAGAA GAAGAGCTGT GTAGTGGAAA GCCCTTTGAT 
GCCTTCACAG ACCTCAAGAA TGGTTCCCTT TTTGCCTTCC GAGGTGAGTA TTGCTATGAG 
CTAGATGAAA CAGCAGTGAG GCCTGGGTAC CCCAAACTCA TCCAAGATGT CTGGGGCATC 
GAGGGCCCCA TCGACGCTGC CTTCACTCGC ATCAACTGTC AGGGGAAGAC CTACTTGTTC 
AAGGGTAGTC AGTACTGGCG CTTTGAGGAT GGTGTCCTGG ACCCTGATTA TCCCCGAAAC 
ATCTCTGAAG GCTTCAGTGG CATACCAGAC AACGTTGACG CAGCCTTAGC CCTTCCTGCC 
CACAGTTACA GTGGCCGGGA AAGGGTCTAC TTCTTCAAGG GAAAACAGTA CTGGGAGTAT 
GAATTTCAGC AGCAGCCCAG CCAGGAGGAA TGTGAAGGAA GTTCTCTGTC AGCCGTGTTT 
GAGCACTTTG CCTTGCTTCA GCGGGACAGC TGGGAGAACA TTTTTGAACT CCTCTTCTGG 
GGCAGATCCT CTGATGGCGC CAAAGGACCC CAATTCATCA GCAGGGATTG GCATGGTGTA 
CCAGGGAAAG TGGACGCTGC TATGGCCGGC CGCATCTACA TCACTGGCTC CACATTCCGC 
TCTGTCCAGG CCAAAAAACA GAAGTCTGGG CGTCGAAGTC GAAAACGCTA CCGTTCACGC 
CGAGGACGTG GCCATAGCCG CAGCCGCAGT CGCAGCATGA GCTCTCGTCG CCCATCGCGT 
TCAGTCTGGT TCTCTTTGCT CTCCAGCGAA GAGAGTGGGC TAGGAACCTA CAATTACGAT 
TATGATATGA ACTGGCGCAT ACCTGCCACC TGCGAGCCCA TTCAGAGCGT CTATTTCTTC 
TCTGGAGACA AGTACTACCG AGTTAACCTT AGAACACGGC GAGTGGACTC TGTGAATCCT 
CCCTACCCAC GCTCCATTGC TCAGTATTGG CTGGGCTGCC CAACCTCTGA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062029.2
CDS	80..1516
Translation	MASLRPFFILALLALVSLADQESCKGRCTQGFMASKKCQCDELCTYYQSCCVDYMEQCKPQVTRGDVFTMPEDEYWSYDYPEETKNSTSTGVQSENTSLHFNLKPRAEETIKPTTPDPQEQSNTQEPEVGQQGVAPRPDTTDEGTSEFPEEELCSGKPFDAFTDLKNGSLFAFRGEYCYELDETAVRPGYPKLIQDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISEGFSGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYEFQQQPSQEECEGSSLSAVFEHFALLQRDSWENIFELLFWGRSSDGAKGPQFISRDWHGVPGKVDAAMAGRIYITGSTFRSVQAKKQKSGRRSRKRYRSRRGRGHSRSRSRSMSSRRPSRSVWFSLLSSEESGLGTYNYDYDMNWRIPATCEPIQSVYFFSGDKYYRVNLRTRRVDSVNPPYPRSIAQYWLGCPTSEK

Target ORF information:

RefSeq Version	NM_019156.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vitronectin (Vtn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019156.2

ORF Insert Sequence:

ATGGCATCTC TGAGGCCCTT TTTCATACTA GCCCTGCTGG CATTGGTTTC TCTGGCTGAC 
CAAGAGTCAT GCAAGGGCCG CTGCACTCAG GGTTTCATGG CCAGCAAGAA GTGTCAATGT 
GATGAGCTTT GCACCTACTA TCAGAGCTGC TGTGTTGACT ACATGGAGCA GTGCAAGCCC 
CAAGTGACCC GGGGGGATGT GTTTACCATG CCAGAAGATG AGTATTGGAG CTATGACTAC 
CCGGAGGAAA CCAAGAACAG TACCAGCACT GGCGTGCAAT CCGAGAACAC CTCTCTACAC 
TTCAACCTAA AGCCTCGAGC CGAAGAGACT ATAAAGCCAA CAACTCCTGA TCCTCAGGAA 
CAGTCAAACA CCCAAGAACC TGAAGTGGGG CAACAGGGAG TGGCCCCAAG GCCTGACACC 
ACTGATGAAG GAACCTCTGA GTTTCCAGAA GAAGAGCTGT GTAGTGGAAA GCCCTTTGAT 
GCCTTCACAG ACCTCAAGAA TGGTTCCCTT TTTGCCTTCC GAGGTGAGTA TTGCTATGAG 
CTAGATGAAA CAGCAGTGAG GCCTGGGTAC CCCAAACTCA TCCAAGATGT CTGGGGCATC 
GAGGGCCCCA TCGACGCTGC CTTCACTCGC ATCAACTGTC AGGGGAAGAC CTACTTGTTC 
AAGGGTAGTC AGTACTGGCG CTTTGAGGAT GGTGTCCTGG ACCCTGATTA TCCCCGAAAC 
ATCTCTGAAG GCTTCAGTGG CATACCAGAC AACGTTGACG CAGCCTTAGC CCTTCCTGCC 
CACAGTTACA GTGGCCGGGA AAGGGTCTAC TTCTTCAAGG GAAAACAGTA CTGGGAGTAT 
GAATTTCAGC AGCAGCCCAG CCAGGAGGAA TGTGAAGGAA GTTCTCTGTC AGCCGTGTTT 
GAGCACTTTG CCTTGCTTCA GCGGGACAGC TGGGAGAACA TTTTTGAACT CCTCTTCTGG 
GGCAGATCCT CTGATGGCGC CAAAGGACCC CAATTCATCA GCAGGGATTG GCATGGTGTA 
CCAGGGAAAG TGGACGCTGC TATGGCCGGC CGCATCTACA TCACTGGCTC CACATTCCGC 
TCTGTCCAGG CCAAAAAACA GAAGTCTGGG CGTCGAAGTC GAAAACGCTA CCGTTCACGC 
CGAGGACGTG GCCATAGCCG CAGCCGCAGT CGCAGCATGA GCTCTCGTCG CCCATCGCGT 
TCAGTCTGGT TCTCTTTGCT CTCCAGCGAA GAGAGTGGGC TAGGAACCTA CAATTACGAT 
TATGATATGA ACTGGCGCAT ACCTGCCACC TGCGAGCCCA TTCAGAGCGT CTATTTCTTC 
TCTGGAGACA AGTACTACCG AGTTAACCTT AGAACACGGC GAGTGGACTC TGTGAATCCT 
CCCTACCCAC GCTCCATTGC TCAGTATTGG CTGGGCTGCC CAACCTCTGA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Sialylation of vitronectin regulates stress fiber formation and cell spreading of dermal fibroblasts via a heparin-binding site.
Glycoconjugate journal33(2)227-36(2016 Apr)
Miyamoto Y,Tanabe M,Date K,Sakuda K,Sano K,Ogawa H

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Cell death and differentiation20(3)369-81(2013 Mar)
Brafman DA,Phung C,Kumar N,Willert K

Purkinje cell maturation participates in the control of oligodendrocyte differentiation: role of sonic hedgehog and vitronectin.
PloS one7(11)e49015(2012)
Bouslama-Oueghlani L,Wehrl? R,Doulazmi M,Chen XR,Jaudon F,Lemaigre-Dubreuil Y,Rivals I,Sotelo C,Dusart I