Wnt8a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wnt8a
Entrez Gene ID	291678
Full Name	Wnt family member 8A
General protein information	Preferred Names Wnt family member 8A Names protein Wnt-8a wingless-related MMTV integration site 8A wingless-type MMTV integration site family, member 8A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106155.1	NP_001099625.1	protein Wnt-8a precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	WNT8A	NP_490645.1
Canis lupus familiaris (dog)	WNT8A	XP_005626641.1
Mus musculus (house mouse)	Wnt8a	NP_033316.1
Rattus norvegicus (Norway rat)	Wnt8a	NP_001099625.1
Danio rerio (zebrafish)	wnt8a	NP_571021.3
	WNT8A	XP_001112480.1
Xenopus tropicalis (tropical clawed frog)	wnt8a	NP_001017208.1
Pan troglodytes (chimpanzee)	WNT8A	XP_527023.3
Bos taurus (cattle)	WNT8A	NP_001179299.1
Gallus gallus (chicken)	WNT8A	NP_990862.1

Gene Ontology
Bibliography

Related articles in PubMed

Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families.
Voloshanenko O, Gmach P, Winter J, Kranz D, Boutros M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(11)4832-4844(2017 Nov)

Endogenous Wnt/beta-catenin signaling is required for cardiac differentiation in human embryonic stem cells.
Paige SL, Osugi T, Afanasiev OK, Pabon L, Reinecke H, Murry CE
PloS one5(6)e11134(2010 Jun)

Alternative wnt signaling is initiated by distinct receptors.
van Amerongen R, Mikels A, Nusse R
Science signaling1(35)re9(2008 Sep)

Variation in WNT genes is associated with non-syndromic cleft lip with or without cleft palate.
Chiquet BT, Blanton SH, Burt A, Ma D, Stal S, Mulliken JB, Hecht JT
Human molecular genetics17(14)2212-8(2008 Jul)

Canonical Wnt signaling and its antagonist regulate anterior-posterior axis polarization by guiding cell migration in mouse visceral endoderm.
Kimura-Yoshida C, Nakano H, Okamura D, Nakao K, Yonemura S, Belo JA, Aizawa S, Matsui Y, Matsuo I
Developmental cell9(5)639-50(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Wnt8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wnt8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa41425	NM_001106155.1 Latest version!	Rattus norvegicus Wnt family member 8A (Wnt8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wnt8a ( NM_001106155.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099625.1 Rattus norvegicus Wnt family member 8A (Wnt8a), mRNA.

Price & Availability↑

CloneID	ORa41425
Clone ID Related Accession (Same CDS sequence)	NM_001106155.1
Accession Version	NM_001106155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	protein Wnt-8a precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473974.1. On or before Oct 4, 2007 this sequence version replaced XM_226051.4, XM_001061171.1.

ATGGTCAGGA CCATGGGACA CTTTTTCATG CTGTGGGTGG CTGCGGGCAT GTGTTATACC 
GCTTTCGGTG CTTCTGCCTG GTCAGTGAAC AATTTCCTGA TAACAGGTCC CAAGGCCTAT 
GTGACCTACA CCGCCAGCGT GGCCTTGGGC GCTCAGATGG GCATGGAAGA GTGTAAGTTC 
CAGTTTGCCT GGGAGCGGTG GAACTGTCCT GAGCACGCTT TCCAGTTTTC AACCCACACC 
AGGCCGAGAG GTGCCACGAG AGAGACATCC TTCATCCATG CCATCCGCTC TGCTGCAGTC 
ATGTACGCTG TCACCAAGAA CTGTAGCATG GGCGACTTGG AAACCTGTGG CTGTGATGAG 
TCAAATAATG GGAAAGCAGG TGGCCATGGC TGGATCTGGG GAGGCTGCAG CGACAATGTG 
GAATTCGGGG AAAAGATCTC CAGACTCTTT GTGGACAGTT TGGAGAAAGG AAAGGATGCC 
AGAGCCCTGA TGAATCTTCA CAACAACAGG GCTGGCAGGC TGGCAGTGAG GGCCTCTATG 
AAAAGAACCT GCAAGTGTCA TGGCATCTCA GGAAGCTGCA GCATCCAGAC GTGTTGGCTG 
CAGCTGGCTG ACTTCCGGCA GATGGGAAAT TACCTAAAGG CCAAGTATGA CCGCGCGCTG 
AAAATTGAGA CGGATAAGCG CCAGCTCAGG GCTGGCAACA GAGCTGAAGG CCGCTGGGCT 
CCCATCGAGG CCTTCCTTCC CAGCGCAGAG GCTGAGCTGA TCTTCTTAGA GGGGTCTCCT 
GACTACTGCA ACCGCAATGC CAGCCTGGGC ATCTACGGCA CAGAGGGGCG GGAGTGCCTG 
CAGAACGCCC GCAGTGCTTC CCGGTGGGAG CAGCGCAGCT GTGGGCGCCT GTGCACCGAG 
TGTGGGCTGC AGGTGGAGGA GAGGCGAACA GAGGCCGTGA GCAGCTGTGA CTGCAACTTT 
CAGTGGTGTT GCACGGTCAA GTGTGGCCAG TGCAGGCGTG TGGTGAACAG GTACTACTGC 
ACACGCCCTG CAGGTAGTGC GGGGCCCCGG GGCACCACCA CAAAGTCACT TGTTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099625.1
CDS	42..1121
Translation	MVRTMGHFFMLWVAAGMCYTAFGASAWSVNNFLITGPKAYVTYTASVALGAQMGMEECKFQFAWERWNCPEHAFQFSTHTRPRGATRETSFIHAIRSAAVMYAVTKNCSMGDLETCGCDESNNGKAGGHGWIWGGCSDNVEFGEKISRLFVDSLEKGKDARALMNLHNNRAGRLAVRASMKRTCKCHGISGSCSIQTCWLQLADFRQMGNYLKAKYDRALKIETDKRQLRAGNRAEGRWAPIEAFLPSAEAELIFLEGSPDYCNRNASLGIYGTEGRECLQNARSASRWEQRSCGRLCTECGLQVEERRTEAVSSCDCNFQWCCTVKCGQCRRVVNRYYCTRPAGSAGPRGTTTKSLVA

Target ORF information:

RefSeq Version	NM_001106155.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Wnt family member 8A (Wnt8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106155.1

ORF Insert Sequence:

ATGGTCAGGA CCATGGGACA CTTTTTCATG CTGTGGGTGG CTGCGGGCAT GTGTTATACC 
GCTTTCGGTG CTTCTGCCTG GTCAGTGAAC AATTTCCTGA TAACAGGTCC CAAGGCCTAT 
GTGACCTACA CCGCCAGCGT GGCCTTGGGC GCTCAGATGG GCATGGAAGA GTGTAAGTTC 
CAGTTTGCCT GGGAGCGGTG GAACTGTCCT GAGCACGCTT TCCAGTTTTC AACCCACACC 
AGGCCGAGAG GTGCCACGAG AGAGACATCC TTCATCCATG CCATCCGCTC TGCTGCAGTC 
ATGTACGCTG TCACCAAGAA CTGTAGCATG GGCGACTTGG AAACCTGTGG CTGTGATGAG 
TCAAATAATG GGAAAGCAGG TGGCCATGGC TGGATCTGGG GAGGCTGCAG CGACAATGTG 
GAATTCGGGG AAAAGATCTC CAGACTCTTT GTGGACAGTT TGGAGAAAGG AAAGGATGCC 
AGAGCCCTGA TGAATCTTCA CAACAACAGG GCTGGCAGGC TGGCAGTGAG GGCCTCTATG 
AAAAGAACCT GCAAGTGTCA TGGCATCTCA GGAAGCTGCA GCATCCAGAC GTGTTGGCTG 
CAGCTGGCTG ACTTCCGGCA GATGGGAAAT TACCTAAAGG CCAAGTATGA CCGCGCGCTG 
AAAATTGAGA CGGATAAGCG CCAGCTCAGG GCTGGCAACA GAGCTGAAGG CCGCTGGGCT 
CCCATCGAGG CCTTCCTTCC CAGCGCAGAG GCTGAGCTGA TCTTCTTAGA GGGGTCTCCT 
GACTACTGCA ACCGCAATGC CAGCCTGGGC ATCTACGGCA CAGAGGGGCG GGAGTGCCTG 
CAGAACGCCC GCAGTGCTTC CCGGTGGGAG CAGCGCAGCT GTGGGCGCCT GTGCACCGAG 
TGTGGGCTGC AGGTGGAGGA GAGGCGAACA GAGGCCGTGA GCAGCTGTGA CTGCAACTTT 
CAGTGGTGTT GCACGGTCAA GTGTGGCCAG TGCAGGCGTG TGGTGAACAG GTACTACTGC 
ACACGCCCTG CAGGTAGTGC GGGGCCCCGG GGCACCACCA CAAAGTCACT TGTTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(11)4832-4844(2017 Nov)
Voloshanenko O,Gmach P,Winter J,Kranz D,Boutros M

Endogenous Wnt/beta-catenin signaling is required for cardiac differentiation in human embryonic stem cells.
PloS one5(6)e11134(2010 Jun)
Paige SL,Osugi T,Afanasiev OK,Pabon L,Reinecke H,Murry CE

Alternative wnt signaling is initiated by distinct receptors.
Science signaling1(35)re9(2008 Sep)
van Amerongen R,Mikels A,Nusse R

Variation in WNT genes is associated with non-syndromic cleft lip with or without cleft palate.
Human molecular genetics17(14)2212-8(2008 Jul)
Chiquet BT,Blanton SH,Burt A,Ma D,Stal S,Mulliken JB,Hecht JT

Canonical Wnt signaling and its antagonist regulate anterior-posterior axis polarization by guiding cell migration in mouse visceral endoderm.
Developmental cell9(5)639-50(2005 Nov)
Kimura-Yoshida C,Nakano H,Okamura D,Nakao K,Yonemura S,Belo JA,Aizawa S,Matsui Y,Matsuo I