Ucn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ucn
Entrez Gene ID	29151
Full Name	urocortin
General protein information	Preferred Names urocortin Names urocortin corticotensin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	binds the corticotropin-releasing factor receptor CRF1; may play a role in learning and memory; may act as a stress response neuropeptide [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019150.1	NP_062023.1	urocortin precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UCN	NP_001027472.1
Mus musculus (house mouse)	Ucn	NP_067265.1
Pan troglodytes (chimpanzee)	UCN	XP_001156027.1
Rattus norvegicus (Norway rat)	Ucn	NP_062023.1
Canis lupus familiaris (dog)	UCN	XP_853760.1
Homo sapiens (human)	UCN	NP_003344.1
	UCN	XP_001092536.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of urocortin as an anti-apoptotic protein in experimental ischemia-reperfusion model of the rat testis.
Sumii K, Miyake H, Enatsu N, Chiba K, Fujisawa M
Biochemical and biophysical research communications479(2)387-392(2016 Oct)

MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression.
Aschrafi A, Verheijen JM, Gordebeke PM, Olde Loohuis NF, Menting K, Jager A, Palkovits M, Geenen B, Kos A, Martens GJ, Glennon JC, Kaplan BB, Gaszner B, Kozicz T
Journal of psychiatry & neuroscience : JPN41(5)342-53(2016 Aug)

Sex and estrogen affect the distribution of urocortin-1 immunoreactivity in brainstem autonomic nuclei of the rat.
Ciriello J
Brain research bulletin11681-92(2015 Jul)

Modulation of learning and anxiety by corticotropin-releasing factor (CRF) and stress: differential roles of CRF receptors 1 and 2.
Radulovic J, R?hmann A, Liepold T, Spiess J
The Journal of neuroscience : the official journal of the Society for Neuroscience19(12)5016-25(1999 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Effects of early life stress and 5-HTT genotype at the molecular level could constitute early life stress x 5-HTT genotype interaction modulating the impact of stress on Edinger-Westphal-Ucn1 and dorsal raphe-5-HT neurons.
Title: Serotonin and urocortin 1 in the dorsal raphe and Edinger-Westphal nuclei after early life stress in serotonin transporter knockout rats.

urocortin may play a cytoprotective role in the germ cells in response to ischemia-reperfusion injury through the activation of major anti-apoptotic proteins, as well as by the mitogen-activated protein kinase signaling pathway activation.
Title: Characterization of urocortin as an anti-apoptotic protein in experimental ischemia-reperfusion model of the rat testis.

Study found reduced miR-326 levels concomitant with elevated Ucn1 levels in Edinger-Westphal nucleus (EWcp) of depressed suicide completers and in the EWcp of depressed rats. In fully recovered rats, both serum and EWcp miR-326 levels rebounded to nonstressed levels. While downregulation of miR-326 levels in primary midbrain neurons enhanced Ucn1 expression levels, miR-326 overexpression selectively reduced its levels.
Title: MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression.

Results suggest that sex differences exist in the distribution of UCN-1 within brainstem autonomic areas, and circulating level of estrogen may play an important role with regards to the function of these UCN-1 neurons during stress responses
Title: Sex and estrogen affect the distribution of urocortin-1 immunoreactivity in brainstem autonomic nuclei of the rat.

UCN might contribute to regulate late midbrain dopaminergic neuron differentiation during ventral midbrain development.
Title: Epigenetic regulation contributes to urocortin-enhanced midbrain dopaminergic neuron differentiation.

The following Ucn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ucn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13322	NM_019150.1 Latest version!	Rattus norvegicus urocortin (Ucn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ucn ( NM_019150.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062023.1 Rattus norvegicus urocortin (Ucn), mRNA.

Price & Availability↑

CloneID	ORa13322
Clone ID Related Accession (Same CDS sequence)	NM_019150.1
Accession Version	NM_019150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Rattus norvegicus(Norway rat)
Product	urocortin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U33935.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U33935.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCAGA GGGGACGCGC TACGCTCCTG GTAGCGTTGC TGCTTCTGGT TCAGCTGCGC 
CCGGAGAGCA GCCAGTGGAG CCCAGCGGCT GCGGCGGCGA ATGTGGTCCA GGATCCGAAT 
CTGCGATGGA ACCCCGGAGT GCGGAATCAG GGCGGAGGTG TCCGCGCACT CCTCTTGCTG 
TTAGCGGAGC GCTTCCCGCG CCGCGCGGGA TCTGAGCCTG CAGGCGAGCG GCAACGACGA 
GACGACCCGC CGTTGTCCAT CGACCTCACC TTCCACCTGC TGCGGACCCT GCTAGAGCTA 
GCTCGGACAC AGAGCCAGCG CGAGCGCGCA GAGCAGAACC GCATCATATT CGATTCGGTG 
GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062023.1
CDS	58..426
Translation	MRQRGRATLLVALLLLVQLRPESSQWSPAAAAANVVQDPNLRWNPGVRNQGGGVRALLLLLAERFPRRAGSEPAGERQRRDDPPLSIDLTFHLLRTLLELARTQSQRERAEQNRIIFDSVGK

Target ORF information:

RefSeq Version	NM_019150.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus urocortin (Ucn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019150.1

ORF Insert Sequence:

ATGAGGCAGA GGGGACGCGC TACGCTCCTG GTAGCGTTGC TGCTTCTGGT TCAGCTGCGC 
CCGGAGAGCA GCCAGTGGAG CCCAGCGGCT GCGGCGGCGA ATGTGGTCCA GGATCCGAAT 
CTGCGATGGA ACCCCGGAGT GCGGAATCAG GGCGGAGGTG TCCGCGCACT CCTCTTGCTG 
TTAGCGGAGC GCTTCCCGCG CCGCGCGGGA TCTGAGCCTG CAGGCGAGCG GCAACGACGA 
GACGACCCGC CGTTGTCCAT CGACCTCACC TTCCACCTGC TGCGGACCCT GCTAGAGCTA 
GCTCGGACAC AGAGCCAGCG CGAGCGCGCA GAGCAGAACC GCATCATATT CGATTCGGTG 
GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of urocortin as an anti-apoptotic protein in experimental ischemia-reperfusion model of the rat testis.
Biochemical and biophysical research communications479(2)387-392(2016 Oct)
Sumii K,Miyake H,Enatsu N,Chiba K,Fujisawa M

MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression.
Journal of psychiatry & neuroscience : JPN41(5)342-53(2016 Aug)
Aschrafi A,Verheijen JM,Gordebeke PM,Olde Loohuis NF,Menting K,Jager A,Palkovits M,Geenen B,Kos A,Martens GJ,Glennon JC,Kaplan BB,Gaszner B,Kozicz T

Sex and estrogen affect the distribution of urocortin-1 immunoreactivity in brainstem autonomic nuclei of the rat.
Brain research bulletin11681-92(2015 Jul)
Ciriello J

Modulation of learning and anxiety by corticotropin-releasing factor (CRF) and stress: differential roles of CRF receptors 1 and 2.
The Journal of neuroscience : the official journal of the Society for Neuroscience19(12)5016-25(1999 Jun)
Radulovic J,R?hmann A,Liepold T,Spiess J