Bsn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bsn
Entrez Gene ID	29138
Full Name	bassoon (presynaptic cytomatrix protein)
Synonyms	Znf231
General protein information	Preferred Names bassoon (presynaptic cytomatrix protein) Names protein bassoon neuronal double zinc finger protein zinc finger protein 231
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	component of the presynaptic cytoskeleton and may be be involved in cytomatrix organization at the site of neurotransmitter release [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019146.2	NP_062019.2	protein bassoon

Pathways from BioSystems

Homologies

	BSN	XP_001094100.2
Canis lupus familiaris (dog)	BSN	XP_541885.2
Mus musculus (house mouse)	Bsn	NP_031593.2
Homo sapiens (human)	BSN	NP_003449.2
Rattus norvegicus (Norway rat)	Bsn	NP_062019.2
Gallus gallus (chicken)	BSN	XP_004944501.1
Pan troglodytes (chimpanzee)	BSN	XP_516463.4
Bos taurus (cattle)	BSN	XP_002697122.2
Xenopus tropicalis (tropical clawed frog)	bsn	XP_002936530.2
Danio rerio (zebrafish)	LOC100004063	XP_001920788.3

Gene Ontology
Bibliography

Related articles in PubMed

Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon.
Terry-Lorenzo RT, Torres VI, Wagh D, Galaz J, Swanson SK, Florens L, Washburn MP, Waites CL, Gundelfinger ED, Reimer RJ, Garner CC
PloS one11(12)e0167535(2016)

Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover.
K?rber C, Horstmann H, Venkataramani V, Herrmannsd?rfer F, Kremer T, Kaiser M, Schwenger DB, Ahmed S, Dean C, Dresbach T, Kuner T
Neuron87(3)521-33(2015 Aug)

Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo.
Ivanova D, Dirks A, Montenegro-Venegas C, Sch?ne C, Altrock WD, Marini C, Frischknecht R, Schanze D, Zenker M, Gundelfinger ED, Fejtova A
The EMBO journal34(8)1056-77(2015 Apr)

Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone.
Gundelfinger ED, Reissner C, Garner CC
Frontiers in synaptic neuroscience719(2015)

Localization of the presynaptic cytomatrix protein Piccolo at ribbon and conventional synapses in the rat retina: comparison with Bassoon.
Dick O, Hack I, Altrock WD, Garner CC, Gundelfinger ED, Brandst?tter JH
The Journal of comparative neurology439(2)224-34(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings provide a mechanism for the presynaptic targeting of Trio and support a model in which Piccolo and Bassoon play a role in regulating neurotransmission through interactions with proteins, including Trio, that modulate the dynamic assembly of F-actin during cycles of synaptic vesicle exo- and endocytosis.
Title: Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon.

CtBP1 is targeted and/or anchored to presynapses by direct interaction with the active zone scaffolding proteins Bassoon and piccolo.
Title: Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo.

a novel role for Bassoon and Piccolo as critical regulators of presynaptic ubiquitination and proteostasis.
Title: Bassoon and Piccolo maintain synapse integrity by regulating protein ubiquitination and degradation.

phosphorylation-induced 14-3-3 binding to Bassoon modulates its anchoring to the presynaptic cytomatrix
Title: Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins.

Bassoon co-localizes with presynaptic calcium channel, modifies channel function, and recovers from aging related loss by exercise.
Title: Active zone protein Bassoon co-localizes with presynaptic calcium channel, modifies channel function, and recovers from aging related loss by exercise.

The following Bsn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bsn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12965	NM_019146.2 Latest version!	Rattus norvegicus bassoon (presynaptic cytomatrix protein) (Bsn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bsn ( NM_019146.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062019.2 Rattus norvegicus bassoon (presynaptic cytomatrix protein) (Bsn), mRNA.

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CloneID	ORa12965
Clone ID Related Accession (Same CDS sequence)	NM_019146.2
Accession Version	NM_019146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	protein bassoon
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07073446.1 and AC128059.4. On Oct 27, 2007 this sequence version replaced NM_019146.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: Y16563.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 9679147, 16373352 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACG AGGCCAGCCT GGAGGGCGGC GCGGGCGAAG GGCCGCTGCC GCCGGGAGGC 
TCTGGTCTGG GTCCGGGCCC CGGAGCAGGG AAGCCGCCTT CAGCACTAGC TGGCGGCGGA 
CAGCTCCCCG TGGCTGGTGC TGCGCGAGCG GCGGGACCCC CGACCCCTGG TCTCGGTCTG 
GTCCCCGGCC CTGGTCCCGG CCCCGGCCCG GGCAGTGTTT CCCGGAGACT GGACCCCAAG 
GAGCCCCTGG GCAGCCAGAG AGCAACTTCT CCAACCCCAA AGCAAGCTTC TGCTACTGCT 
CCTGGCCGAG AGAGCCCCCG AGAGACAAGG GCCCAAGGCC TGTCAGGCCA GGAGGCCGAA 
GGTCCCCGCA GGACACTGCA GGTAGACAGC AGGACACAGA GATCAGGACG GTCCCCCTCC 
GTGTCACCAG ACAGAGGCAG CACTCCCACA TCACCCTATT CCGTCCCCCA GATTGCCCCT 
CTTCCCAGCA GCACCCTGTG TCCAATATGC AAGACATCGG ACCTCACATC AACCTCCAGC 
CAGCCAAACT TCAACACCTG CACCCAGTGT CACAACAAGG TCTGCAACCA ATGTGGGTTC 
AACCCCAACC CTCACCTCAC CCAGGTGAAG GAGTGGCTCT GTTTGAACTG TCAGATGCAG 
AGGGCCCTGG GAATGGACAT GACCACTGCA CCTCGGTCCA AGAGCCAGCA GCAGCTACAC 
TCCCCGGCCC TGTCTCCTGC CCACTCCCCA GCCAAACAGC CTCTGGGGAA GCCAGAGCAA 
GAGAGATCTC GGAGCCCAGG GGCCACACAG TCTGGTCCCC GCCAGGCTGA AGCAGCCAGG 
GCCACCTCAG TGCCTGGGCC TACCCAGGCA ACTGCCCCTC CAGAGGTGGG GAGGGTGTCT 
CCTCAGCCCC CTCTCTCTAC CAAGCCTTCC ACAGCTGAGC CCAGGCCACC TGCAGGAGAG 
GCCCAGGGCA AAAGTGCCAC CACAGTGCCC TCCGGGCTTG GTGCTGCTGA ACAGACCCAG 
GAGGGGCTCA CCGGGAAGCT CTTTGGCCTT GGAGCATCAC TGCTGACCCA GGCAAGCACC 
CTTATGTCTG TGCAGCCAGA GGCTGACACC CAGGGCCAGC CTTCCCCCAG CAAGGGGCCA 
CCCAAGATTG TCTTCAGTGA TGCCAGCAAG GAGGCGGGTC CAAGACCCCC AGGCTCAGGG 
CCCGGGCCTG GGCCAACCCC TGGAGCCAAA ACGGAGCCTG GGCCTAGAAC AGGTCCTGGA 
TCAGGGCCTG GGGCCCTGGC AAAAACTGGA GGAACCCCCA GTCCAAAGCA TGGCAGAGCA 
GACCATCAGG CAGCATCCAA AGCTGCTGCC AAGCCAAAGA CCATGCCGAA GGAAAGGGCT 
GCCTGCCCAC TGTGCCAAGC CGAGCTCAAC GTGGGTAGCA GGGGCCCAGC CAACTACAAT 
ACCTGCACTG CCTGCAAGCT CCGGGTGTGC ACCCTGTGTG GCTTTAACCC GACACCACAC 
CTGGTGGAGA AAACAGAGTG GCTCTGTCTG AACTGCCAAA CCAAGAGGCT GCTGGAGGGC 
AGCTTGGGGG AGCCGGCCCC CCTGCCTCTG CCCACCCCAC AGCAGCCTCC AGCAGGGGTC 
CCTCAGCGAG CAGCTGGAGC AAGTCCTCTG AAGCAGAAAG GGCCACAGGG GCCGGGCCAG 
CCCTCAGGCT CCCTGCCTCC CAAGGCCAGT CCTCAAGCCG CCAAGGCCAG CCCTCAGGCC 
GCCAAGGCCA GCCCTCAGGC CAAGCCTCTC AGGGCTTCTG AACCCAGCAA GACCTCTAGC 
AGCGCCCCGG AAAAGAAGAC AGGGATCCCT GTGAAAGCTG AGCCTGTGCC GAAGCCACCT 
CCAGAGACTG CTGTGCCTCC TGGGACTCCT AAAGCAAAAA GCGGGGTGAA GAGGACCGAC 
CCTGCCACCC CAGTCGTCAA GCCTGTTCCA GAAGCTCCCA AGAGCGGGGA GGCTGAGGAA 
CCTGTCCCCA AGCCTTACTC TCAGGATCTG TCTCGAAGCC CACAAAGCCT CAGTGATACA 
GGCTACTCCT CCGATGGCGT CTCCAGCTCC CAGAGTGAGA TCACAGGTGT TGTACAGCAA 
GAAGTGGAGC AACTTGACAG TGCGGGGGTG ACAGGCCCAC GTCCACCCAG CCCCTCAGAG 
CTCCACAAAG TGGGCAGTAG TATGCGCCCT TCCCTGGAGG CCCAGGCTGT GGCTCCCAGT 
GGTGAGTGGA GCAAGCCACC CAGCGGCAGT GCTGTTGAAG ACCAGAAACG GCGTCCCCAC 
TCCCTGTCCA TCATGCCCGA GGCCTTCGAC TCTGACGAGG AGCTGGGGGA TATTCTAGAA 
GAGGATGACT CCTTGGCATG GGGGCGTCAG AGGGAGCAGC AAGACACAGC TGAGTCTTCA 
GATGACTTTG GCAGCCAGCT GAGGCATGAC TACGTGGAAG ACAGCAGTGA AGGCGGCTTG 
TCTCCCCTTC CACCTCAGCC CCCAGCCAGG GCAGATATGA CTGATGAAGA GTTCATGCGG 
CGGCAGATCC TAGAAATGAG CGCCGAGGAA GACAACTTGG AGGAAGACGA CACCGCTGTC 
TCCGGGCGAG GCCTGGCCAA ACACGGTGCC CAGAAGGCAA GTGCCAGACC CAGGCCCGAA 
TCTAGCCAAG AATCAGTAGC ACTGCCCAAG AGGCGCCTGC CCCATAATGC CACCACAGGT 
TACGAGGAAC TTCTGTCTGA GGAAGGCCCA GCAGAGCCCA CTGACGGAGC CCTTCAGGGA 
GGTCTCCGAC GCTTTAAAAC TATTGAGCTC AACAGCACAG GTAGCTATGG TCACGAGCTA 
GACCTGGGCC AAGGCTCTGA TCCCAACCTA GACCGGGAGC CTGAGCTGGA GATGGAGAGC 
CTTACAGGCT CGCCTGAAGA CCGCTCTCGT GGGGAGCACT CCTCTACACT GCCGGCTTCC 
ACGCCTAGCT ATACATCTGG CACCTCACCC ACCTCCCTGT CTTCCCTGGA GGAGGACAGT 
GACAGCAGCC CCAGCCGCAG GCAGCGGCTA GAAGAAGCGA AGCAGCAGCG CAAGGCCCGG 
CACCGGTCCC ACGGGCCCCT GCTGCCCACC ATTGAGGACT CTTCCGAGGA GGAGGAGCTG 
CGGGAAGAAG AGGAGCTGCT GCGTGAGCAA GAGAAGATGC GGGAGGTGGA ACAGCAGCGA 
ATTCGAAGCA CAGCCCGCAA GACCCGGCGT GACAAGGAAG AACTTCGGGC TCAGCGGCGG 
CGTGAGCGCT CCAAGACACC GCCTAGTAAC CTGTCACCCA TCGAAGATGC GTCCCCTACA 
GAGGAGCTGA GGCAGGCAGC GGAGATGGAG GAGCTACACC GCTCTTCCTG CTCTGAGTAC 
TCCCCCTCCC CCTCCCTGGA CTCAGAGGCT GAGACCCTCG ATGGCGGCCC CACTCGGCTC 
TACAAGTCGG GCAGCGAGTA CAACCTGCCT GCCTTTATGT CCCTCTGCTC ACCAACCGAG 
ACCCCTTCAG GCAGTTCCAC CACGCCCAGT TCTGGACGGC CCCTTAAGAG TGCGGAGGAG 
GCCTACGAGG ACATGATGAG AAAAGCTGAG CTGCTCCAGA GACAGCAGGG CCAGGCGGCA 
GGGGCCCGGG GACCCCATGG CGGCCCCTCT CAGCCCACAG GCCCCCGCAG CCAGGGCTCC 
TTTGAATACC AAGACACCCA AGACCATGAC TATGGCGGCA GGGCTTCTCA GCCTGCGGCA 
GATGGCACGC CCGCAGGCCT CGGAGCAACA GTGTATGAGG AGATCCTTCA GACATCCCAG 
AGCATAGCCC GGATGCGGCA GGCCTCCTCG CGGGATCTGG CCTTCACTGA GGACAAAAAG 
AAGGAGAAGC AGTTTCTGAA TGCGGAGAGT GCATACATGG ACCCAATGAA GCAAAATGGT 
GGCCCACTTA CCCCTGGGAC TAGCCCCACC CAACTTGCTG CTCCTGTGTC ATTTCCCACT 
TCCACCTCCT CAGACAGCAG CGGGGGCCGA GTTATTCCCG ATGTCAGAGT CACTCAGCAT 
TTTGCAAAAG AGCCTCAGGA GCCTCTCAAG CTACATAGCT CTCCTGCCTC TCCCAGTCTG 
GCCTCTAAGG AGGTAGGCAT GACCTTCTCC CAGGGTCCTG GAACTCCAGC CACCACAGCC 
ATGGCGCCTT GTCCAGCCAG CCTTCCACGG GGGTATATGA CTCCAGCCGG CCCTGAGCGC 
AGCCCATCAA CATCTTCCAC AATACACAGC TATGGACAGC CCCCAACCAC TGCTAACTAT 
GGGTCTCAGA CGGAGGAGCT GCCTCACGCT CCCAGTGGCC CTGCTGGAAG TGGGCGGGCC 
TCCAGAGAGA AGCCTCTGAG TGGGGGTGAT GGTGAGGTTG GTCCCCCCCA GCCTTCTCGG 
GGATATTCTT ATTTTACGGG CTCTAGCCCA CCTCTCTCTC CATCCACCCC CTCCGAGAGC 
CCCACCTTCT CTCCAAGCAA GCTGGGTCCA AGGGCCACAG CAGAGTTCTC TACACAGACG 
CCAAGCCTAA CGCCTTCCTC TGACATTCCA CGGAGCCCTG GCACCCCTTC CCCCATGGTA 
GCCCAAGGCA CGCAGACGCC ACACCGACCT AGCACTCCTC GCTTGGTGTG GCAGCAGTCT 
TCCCAGGAGG CTCCTGTTAT GGTCATCACG CTAGCTTCAG ATGCTTCTAG CCAGACCCGA 
ATGGTACACG CCAGTGCCTC TACTTCCCCT CTGTGCTCGC CTACGGACTC TCAGCCTGCC 
TCCCATAGCT ACAGCCAGAC CACACCCCCA AGTGCATCTC AAATGCCCTC AGAACCAGCT 
GGGCCACCTG GTTTCCCACG AGCACCCAGT GCTGGTGTAG ACGGGCCATT GGCGCTGTAT 
GGCTGGGGTG CCCTCCCTGC TGAAAACATC TCCTTATGCC GGATCTCCTC TGTCCCTGGA 
ACATCTAGAG TTGAGCCGGG CCCCAGGCCC CCGGGCACCG CAGTGGTCGA CCTTCGCACG 
GCTGTCAAGC CCACACCCAT TATCCTTACC GACCAGGGCA TGGATCTGAC CTCTCTTGCT 
GTGGAAGCAA GGAAGTATGG CCTTGCCCTG GATCCAGTTC CAGGCCGCCA GTCAACCGCC 
GTGCAGCCTC TAGTTATTAA CCTCAATGCC CAAGAGCAGA CCCATACCTT CCTGGCCACT 
GCCACCACAG TGAGCATCAC CATGGCCTCA TCTGTGCTCA TGGCGCAGCA AAAGCAACCA 
GTGGTATATG GAGATCCTTT CCAGAGCCGC CTCGACTTTG GCCAGGGTTC AGGTAGCCCT 
GTGTGCCTGG CCCAGGTCAA GCAAGTAGAG CAGGCTGTCC AGACAGCCCC ATACCGAGGT 
GGGCCCAGGG GAAGACCCAG GGAGGCTAAG TTCGCCAGGT ATAACCTTCC CAACCAGGTA 
ACACCTCTGG CCAGAAGGGA CATTCTGATC ACTCAGATGG GCACTGCCCA GAGTGTTAGC 
CTGAAGCCAG GACCAGTGCC AGAGCCTGGT GCTGAACCCC ACCGGGCTAC TCCTGCAGAG 
CTCCGGGCAC ATGCTCTACC AGGTACCAGG AAGCCACACA CAGTGGTGGT GCAGATGGGA 
GAGGGCGCAG CCGGCACGGT GACTACCCTG CTCCCAGAAG AGCCAGCAGG AGCCCTGGAC 
CTCACTGGGA TGAGGCCCGA GAGCCGACTG GCATGCTGTG ACATGGCCTA TAAGTTTCCC 
TTTGGCAGTA GCTGCACTGG TACCTTCCAT CCTGCCCCTA GTGCACCTGA CAAGAGTGTG 
ACAGATGCTG CCCTGCCTGG CCAAAGCAGT GGCCCCTTCT ACAGTCCCAG AGACCCTGAG 
CCTCCTGAGC CCCTCACCTT CCGGGCACAA GGGGTCGTAG GACCTGGGCC CCATGAAGAA 
CAGAGGCCCT ACCCACAGGG CTTGCCCGGT AGGCTCTACT CCTCCATGTC TGACACCAAT 
TTGGCTGAAG CTGGTCTCAA CTACCACGCC CAGAGGATTG GGCAGCTCTT CCAGGGCCCT 
GGGAGGGACT CAGCTGTGGA TCTCAGCTCG CTGAAGCATT CTTACAGCTT AGGCTTCGCT 
GATGGACGCT ACTTGGGGCA GGGCTTACAG TATGGCTCGT TCACGGACCT GCGCCACCCC 
ACAGACCTTT TGTCTCACCC ACTGCCCATG AGGCGCTACA GCTCTGTGTC AAACATCTAT 
TCGGATCACC GGTACGGCCC ACGGGGAGAT GCCGTCGGCT TTCAGGAGGC CAGCCTGGCC 
CAGTACAGTG CCACCACAGC CCGCGAGATC AGCCGCATGT GTGCCGCCCT CAACTCCATG 
GACCAGTACG GTGGGCGGCA TGGCGGTGGA AGTGGTGGGC CCGACCTTGT GCCGTATCAG 
CCCCAGCACG GGCCTGGACT CAATGCTCCA CAGGGTCTGG CTTCCCTTAG ATCTGGCCTC 
CTCGGCAACC CCACCTACCC AGAAGGGCAA CCAAGTCCTG GGAACCTTGC TCAGTACGGG 
CCTGCAGCAA GCCAAGGAAC AGCAGTCAGA CAGTTGCTCC CATCCACAGC AACAGTGCGT 
GCAGCCGATG GCATGATCTA CTCGACTATC AATACCCCAA TCGCTGCAAC ACTGCCCATC 
ACCACCCAGC CTGCCTCAGT ACTGCGGCCC ATGGTGCGTG GTGGCATGTA CAGGCCTTAC 
GGATCTGGCG GAGTCACAGC TGTGCCCCTT ACCAGCCTGA CCCGGGTGCC CATGATTGCC 
CCTCGGGTAC CTCTTGGACC AGCAGGGTTA TACCGCTATC CTGCACCAAG TCGATTCCCC 
ATCGCTTCCA CCATTCCACC CGCTGAAGGA CCTGTCTATT TGGGAAAGCC AGCAGCCGCC 
AAGGCCTCGG GGGCAGGAGG CCCGCCAAGG CCTGAGCTAC CAGCAGGGGG TGCTCGAGAA 
GAACCTCTCT CCACAACTGC CCCTCCTGCT GTCATCAAGG AAGCCCCGGT GGCCCAGGCC 
CCGGCCCCAC CACCAGGCCA AAAGCCAGCA GGAGATGCTG CTGCTGGGAG TGGCAGTGGA 
GTCCTCGGCC GGCCTGTGAT GGAGAAGGAG GAGGCATCTC AGGAGGACCG GCAGCGAAAG 
CAACAGGAGC AGCTGCTGCA GCTGGAGCGG GAACGGGTAG AGTTGGAAAA GCTCCGGCAG 
TTGCGGCTAC AGGAGGAGCT GGAGCGAGAG AGGGTGGAGC TGCAGAGGCA TCGTGAGGAG 
GAGCAGCTGC TGGTGCAGAG AGAGTTGCAG GAGCTCCAGA CCATCAAGCA CCATGTTCTG 
CAGCAGCAGC AAGAAGAGCG CCAAGCTCAG TTCGCACTGC AGCGCGAGCA GCTGGCACAG 
CAGCGGCTGC AGCTAGAGCA GATCCAGCAA CTGCAGCAGC AGCTGCAGCA GCAGCTAGAG 
GAGCAGAAAC AGAGGCAAAA GGCCCCCTTT CCTGCAACCT GTGAGGCGCC TAGCCGAGGG 
CCTCCCCCCG CTGCTACCGA GCTGGCCCAG AACGGCCAGT ATTGGCCACC ATTGACCCAC 
ACAGCCTTCA TCGCCGTGGC AGGAACCGAA GGGCCTGGGC AAGCTCGTGA GCCCGTGCTG 
CACAGGGGCC TCCCCAGCTC TGCCTCAGAC ATGTCCCTGC AAACTGAGGA GCAGTGGGAG 
GCAGGCCGTA GTGGCATCAA GAAACGTCAC TCTATGCCAC GCCTGAGAGA CGCCTGTGAA 
CCAGAGTCAG GCCCTGATCC CAGCACGGTC AGGAGGATTG CAGACAGCAG TGTTCAGACA 
GATGATGAGG AGGGCGAGGG CCGCTACCTC CTGACCCGTA GGCGCAGGAC ACGGCGCAGT 
GCCGACTGCA GTGTGCAGAC AGATGACGAG GACAATGCCG AATGGGAGCA GCCTGTCCGC 
CGCCGCAGGT CTCGTCTTTC CCGTCACTCA GACTCAGGCT CTGACAGCAA GCACGAGGCC 
TCGGCCTCAT CGTCCGCTGC TGCCGCTGCG GCGAGGGCCA TGAGCAGTGT GGGTATCCAG 
ACCATCAGTG ACTGCTCTGT ACAGACAGAG CCTGAGCAGC TGCCCAGGGT CTCACCAGCC 
ATCCACATCA CAGCCGCCAC TGACCCAAAG GTGGAGATCG TCAGATACAT ATCAGCACCA 
GAAAAGACTG GGCGAGGGGA GAGCCTGGCC TGCCAGACAG AACCCGATGG GCAGGCTCAG 
GGTGTGGCTG GGCCACAGCT CATAGGACCA ACTGCCATCA GCCCCTACCT ACCTGGCATC 
CAGATAGTCA CCCCAGGAGC CCTAGGCAGA TTTGAAAAGA AGAAGCCAGA TCCTCTGGAG 
ATTGGGTACC AGGCCCACCT GCCCCCGGAG TCCCTGTCGC AGCTTGTGAG CCGCCAGCCT 
CCCAAGTCTC CACAAGTGCT CTACTCACCA GTGTCGCCAC TGTCCCCACA CCGGCTCCTG 
GACACCTCGT TTGCTTCCAG TGAGAGGCTG AACAAGGCTC ATGTGAGTCC CCAGAAGCAG 
TTCATAGCTG ACAGCACTCT TCGCCAGCAG ACTCTGCCGC GCCCCATGAA GACCCTGCAG 
CGGTCCCTGT CCGACCCTAA GCCCCTGAGC CCCACCGCCG AGGAGTCTGC CAAAGAGAGA 
TTCTCCCTCT ACCAGCACCA GGGGGGACTA GGTAGCCAGG TGTCGGCGCT GCCACCCAAC 
GGCCTGGTCC GCAAGGTGAA GCGGACACTG CCCAGCCCCC CTCCAGAGGA AGCTCACCTT 
CCCCTGGCTG GCCAGGTGCC CTCACAGCTG TATGCAGCCA GTCTGCTGCA GCGAGGGTTG 
GCGGGGCCCA CCACCGTCCC TGCTACCAAG GCCAGCCTGC TCCGGGAGCT GGACCGGGAC 
CTGCGGCTGG TGGAGCATGA ATCCACCAAG CTGCGCAAGA AGCAGGCGGA GTTGGACGAG 
GAGGAGAAGG AGATTGATGC CAAGCTCAAG TACCTGGAGC TGGGCATCAC CCAGCGCAAA 
GAGTCTTTGG CCAAAGACCG GGTTGGCCGT GACTACCCAC CTTTACGTGG CCTTGGTGAG 
CATCGTGACT ACCTGTCTGA CAGCGAGCTC AACCAGCTGC GGCTCCAGGG TTGTACCACG 
CCCGCTGGCC AGTATGTGGA CTACCCTGCC TCAGCTGCTG TGCCTGCCAC CCCCTCTGGC 
CCCACTGCCT TCCAACAGCC CCGGTTCCCA CCTGCAGCCA CACAGTACAC TGCAGGCAGT 
AGTGGACCAA CTCAGAATGG ATTTCTAGCC CACCAGGCAC CTACCTACAC TGGCCCTAGC 
ACGTACCCAG CACCTACCTA CCCTCCTGGA ACGAGTTATC CAGCTGAGCC TGGCCTGCCA 
AGCCAACCGG CTTTCCACCC CACAGGCCAT TACGCAGCCC CAACGCCCAT GCCAACCACG 
CAGAGCGCCC CTTTTCCAGT CCAAGCTGAC AGTCACGCTG CCCACCAGAA GCCGCGCCAG 
ACCTCACTGG CTGATTTGGA GCAGAAGGTT CCCACCAATT ATGAAGTGAT CAGCAGCCCT 
GCTGTAACCG TGTCCTCAAC CCCCTCTGAG ACCGGCTACA GTGGTCCAGC TGTGAGCAGC 
AGCTACGAGC ACGGTAAAGC CCCTGAGCAT CCCCGGGGTG GTGATCGAAG CAGTGTGAGC 
CAGAGCCCAG CCCCTACATA TCCCTCTGAC TCCCATTACA CCAGTCTGGA GCAGAACGTT 
CCCCGAAACT ATGTGATGAT CGATGACATC AGTGAGCTAA CCAAGGACAG CACCCCCACT 
GCCTCGGATA GCCAACGGCC AGAGCCCTTG GGCCCAGGTG GAGTCAGTGG GCGTCCCGGG 
AAGGATCCCG GAGAACCAGC TGTCCTAGAG GGACCCACTC TGCCCTGCTG CTATGGCAGA 
GGTGAGGAAG AATCTGAGGA GGACTCATAT GACCCCCGTG GCAAATCTGG TCATCACCGG 
AGCATGGAGA GCAACGGCCG ACCAGCCAGC ACCCACTATT ACAGTGACAG TGACTATAGA 
CATGGGGCTC GAGCAGACAA GTATGGCCCA GGACCCATGG GGCCAAAGCA TCCCTCTAAG 
AACCTGGCCC CGGCTGCCAT CTCCTCCAAG CGCAGCAAGC ACCGGAAGCA AGGCATGGAG 
CAAAAGATCT CCAAGTTCTC ACCTATCGAG GAGGCCAAGG ACGTGGAATC AGACCTGGCC 
TCCTATCCCC CACCCACCGT CAGCAGCAGC CTGACCTCTC GGAGCAGAAA GTTCCAAGAC 
GAAATCACCT ATGGGCTCAA GAAGAATGTG TATGAACAGC AGAGGTACTA CGGGGTGTCC 
AGCCGGGACA CAGCCGAGGA GGATGACCGC ATGTATGGTG GTAGCAGCCG GTCCCGAGTG 
GCATCGGCAT ACAGTGGGGA GAAGCTGTCC AGCCATGATT TCAGCAGCCG AAGCAAAGGG 
TATGAACGGG AACGGGAGAC TGCAGAGCGA CTTCAAAAGG CGGGGCCCAA GCCCTCATCC 
CTGAGCATGG CTCATGGACG GGCACGGCCC CCCATGAGGA GCCAGGCCTC TGAAGAGGAG 
AGCCCTGTCA GCCCCTTAGG GCGGCCCCGT CCCGCAGGGG GCGCTCTTCC TCCTGGGGAT 
ACCTGCCCAC AGTTCTGCTC CAGCCACTCC ATGCCGGACG TCCAGGAGCA CGTCAAGGAC 
GGACCGCGCG CCCACGCATA TAAGCGTGAG GAGGGCTACA TCTTGGATGA CTCCCACTGC 
GTGGTTTCGG ACAGTGAAGC GTATCACCTG GGCCAGGAGG AGACAGACTG GTTTGATAAG 
CCCCGAGATG CCCGCTCCGA CCGGTTCAGG CACCATGGGG GGCATACAGT CTCCTCCTCC 
CAGAAGCGAG GCCCTGCCAG GCACAGCTAC CATGACTACG ATGAGCCCCC TGAGGAGGGC 
CTGTGGCCTC ATGATGAGGG TGGTCCAGGC CGGCATACCT CAGCCAAGGA GCACCGGCAC 
CACGGTGACC ACGGGAGGCA CTCAGGCCGC CATGCTGGTG AGGAGCCAGG GCGCCGTGCT 
GCCAGACCAC ATGCTCGGGA CATGGGTCGC CACGAGACCC GGCCTCACCC TCAGGCCAGC 
CCTGCCCCTG CCATGCAAAA GAAGGGTCAG CCTGGGTACC CCAGCTCTGC TGATTACTCA 
CAGCCATCCC GGGCTCCGTC AGCATACCAC CATGCCTCTG ACAGCAAAAA GGGCTCCCGG 
CAGGCCCACT CTGGGCCTAC TGTACTGCAG CCAAAGCCAG AAGCCCAGGC ACAGCCACAG 
ATGCAAGGTC GGCAGGCGGT TCCGGGACCG CAGCAATCAC AGCCACCATC GTCCAGGCAG 
ACGCCCTCAG GCACAGCATC ACGCCAGCCA CAGACACAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA ACAGCAGCAA CAGCAAGGTC TTGGGCAACA AGCTCCCCAG 
CAGGCTCCAT CACAGGCTCG GCTACAGCAA CAGAGCCAGC CAACCACCCG GAGCACAGCC 
CCTGCTGCCA GCCACCCAGC AGGGAAACCT CAACCAGGCC CCACCACAGC CCCAGGGCCC 
CAGCCAGCAG GACTGCCACG TGCAGAGCAG GCAGGCAGCT CCAAACCGGC AGCCAAAGCG 
CCCCAGCAGG GGCGCGCTCC TCAGGCCCAG TCAGCTCCAG GACCTGCAGG GGCGAAGACT 
GGAGCCAGGC CTGGGGGGAC CCCAGGGGCT CCTGCTGGTC AGCCAGCGGC AGAAGGAGAG 
AGTGTATTTT CTAAGATCCT CCCTGGTGGG GCAGCAGAGC AAGCCGGAAA GCTGACAGAA 
GCTGTCTCTG CTTTTGGCAA AAAATTTTCC TCATTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062019.2
CDS	102..11903
Translation	MGNEASLEGGAGEGPLPPGGSGLGPGPGAGKPPSALAGGGQLPVAGAARAAGPPTPGLGLVPGPGPGPGPGSVSRRLDPKEPLGSQRATSPTPKQASATAPGRESPRETRAQGLSGQEAEGPRRTLQVDSRTQRSGRSPSVSPDRGSTPTSPYSVPQIAPLPSSTLCPICKTSDLTSTSSQPNFNTCTQCHNKVCNQCGFNPNPHLTQVKEWLCLNCQMQRALGMDMTTAPRSKSQQQLHSPALSPAHSPAKQPLGKPEQERSRSPGATQSGPRQAEAARATSVPGPTQATAPPEVGRVSPQPPLSTKPSTAEPRPPAGEAQGKSATTVPSGLGAAEQTQEGLTGKLFGLGASLLTQASTLMSVQPEADTQGQPSPSKGPPKIVFSDASKEAGPRPPGSGPGPGPTPGAKTEPGPRTGPGSGPGALAKTGGTPSPKHGRADHQAASKAAAKPKTMPKERAACPLCQAELNVGSRGPANYNTCTACKLRVCTLCGFNPTPHLVEKTEWLCLNCQTKRLLEGSLGEPAPLPLPTPQQPPAGVPQRAAGASPLKQKGPQGPGQPSGSLPPKASPQAAKASPQAAKASPQAKPLRASEPSKTSSSAPEKKTGIPVKAEPVPKPPPETAVPPGTPKAKSGVKRTDPATPVVKPVPEAPKSGEAEEPVPKPYSQDLSRSPQSLSDTGYSSDGVSSSQSEITGVVQQEVEQLDSAGVTGPRPPSPSELHKVGSSMRPSLEAQAVAPSGEWSKPPSGSAVEDQKRRPHSLSIMPEAFDSDEELGDILEEDDSLAWGRQREQQDTAESSDDFGSQLRHDYVEDSSEGGLSPLPPQPPARADMTDEEFMRRQILEMSAEEDNLEEDDTAVSGRGLAKHGAQKASARPRPESSQESVALPKRRLPHNATTGYEELLSEEGPAEPTDGALQGGLRRFKTIELNSTGSYGHELDLGQGSDPNLDREPELEMESLTGSPEDRSRGEHSSTLPASTPSYTSGTSPTSLSSLEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLREQEKMREVEQQRIRSTARKTRRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSPSLDSEAETLDGGPTRLYKSGSEYNLPAFMSLCSPTETPSGSSTTPSSGRPLKSAEEAYEDMMRKAELLQRQQGQAAGARGPHGGPSQPTGPRSQGSFEYQDTQDHDYGGRASQPAADGTPAGLGATVYEEILQTSQSIARMRQASSRDLAFTEDKKKEKQFLNAESAYMDPMKQNGGPLTPGTSPTQLAAPVSFPTSTSSDSSGGRVIPDVRVTQHFAKEPQEPLKLHSSPASPSLASKEVGMTFSQGPGTPATTAMAPCPASLPRGYMTPAGPERSPSTSSTIHSYGQPPTTANYGSQTEELPHAPSGPAGSGRASREKPLSGGDGEVGPPQPSRGYSYFTGSSPPLSPSTPSESPTFSPSKLGPRATAEFSTQTPSLTPSSDIPRSPGTPSPMVAQGTQTPHRPSTPRLVWQQSSQEAPVMVITLASDASSQTRMVHASASTSPLCSPTDSQPASHSYSQTTPPSASQMPSEPAGPPGFPRAPSAGVDGPLALYGWGALPAENISLCRISSVPGTSRVEPGPRPPGTAVVDLRTAVKPTPIILTDQGMDLTSLAVEARKYGLALDPVPGRQSTAVQPLVINLNAQEQTHTFLATATTVSITMASSVLMAQQKQPVVYGDPFQSRLDFGQGSGSPVCLAQVKQVEQAVQTAPYRGGPRGRPREAKFARYNLPNQVTPLARRDILITQMGTAQSVSLKPGPVPEPGAEPHRATPAELRAHALPGTRKPHTVVVQMGEGAAGTVTTLLPEEPAGALDLTGMRPESRLACCDMAYKFPFGSSCTGTFHPAPSAPDKSVTDAALPGQSSGPFYSPRDPEPPEPLTFRAQGVVGPGPHEEQRPYPQGLPGRLYSSMSDTNLAEAGLNYHAQRIGQLFQGPGRDSAVDLSSLKHSYSLGFADGRYLGQGLQYGSFTDLRHPTDLLSHPLPMRRYSSVSNIYSDHRYGPRGDAVGFQEASLAQYSATTAREISRMCAALNSMDQYGGRHGGGSGGPDLVPYQPQHGPGLNAPQGLASLRSGLLGNPTYPEGQPSPGNLAQYGPAASQGTAVRQLLPSTATVRAADGMIYSTINTPIAATLPITTQPASVLRPMVRGGMYRPYGSGGVTAVPLTSLTRVPMIAPRVPLGPAGLYRYPAPSRFPIASTIPPAEGPVYLGKPAAAKASGAGGPPRPELPAGGAREEPLSTTAPPAVIKEAPVAQAPAPPPGQKPAGDAAAGSGSGVLGRPVMEKEEASQEDRQRKQQEQLLQLERERVELEKLRQLRLQEELERERVELQRHREEEQLLVQRELQELQTIKHHVLQQQQEERQAQFALQREQLAQQRLQLEQIQQLQQQLQQQLEEQKQRQKAPFPATCEAPSRGPPPAATELAQNGQYWPPLTHTAFIAVAGTEGPGQAREPVLHRGLPSSASDMSLQTEEQWEAGRSGIKKRHSMPRLRDACEPESGPDPSTVRRIADSSVQTDDEEGEGRYLLTRRRRTRRSADCSVQTDDEDNAEWEQPVRRRRSRLSRHSDSGSDSKHEASASSSAAAAAARAMSSVGIQTISDCSVQTEPEQLPRVSPAIHITAATDPKVEIVRYISAPEKTGRGESLACQTEPDGQAQGVAGPQLIGPTAISPYLPGIQIVTPGALGRFEKKKPDPLEIGYQAHLPPESLSQLVSRQPPKSPQVLYSPVSPLSPHRLLDTSFASSERLNKAHVSPQKQFIADSTLRQQTLPRPMKTLQRSLSDPKPLSPTAEESAKERFSLYQHQGGLGSQVSALPPNGLVRKVKRTLPSPPPEEAHLPLAGQVPSQLYAASLLQRGLAGPTTVPATKASLLRELDRDLRLVEHESTKLRKKQAELDEEEKEIDAKLKYLELGITQRKESLAKDRVGRDYPPLRGLGEHRDYLSDSELNQLRLQGCTTPAGQYVDYPASAAVPATPSGPTAFQQPRFPPAATQYTAGSSGPTQNGFLAHQAPTYTGPSTYPAPTYPPGTSYPAEPGLPSQPAFHPTGHYAAPTPMPTTQSAPFPVQADSHAAHQKPRQTSLADLEQKVPTNYEVISSPAVTVSSTPSETGYSGPAVSSSYEHGKAPEHPRGGDRSSVSQSPAPTYPSDSHYTSLEQNVPRNYVMIDDISELTKDSTPTASDSQRPEPLGPGGVSGRPGKDPGEPAVLEGPTLPCCYGRGEEESEEDSYDPRGKSGHHRSMESNGRPASTHYYSDSDYRHGARADKYGPGPMGPKHPSKNLAPAAISSKRSKHRKQGMEQKISKFSPIEEAKDVESDLASYPPPTVSSSLTSRSRKFQDEITYGLKKNVYEQQRYYGVSSRDTAEEDDRMYGGSSRSRVASAYSGEKLSSHDFSSRSKGYERERETAERLQKAGPKPSSLSMAHGRARPPMRSQASEEESPVSPLGRPRPAGGALPPGDTCPQFCSSHSMPDVQEHVKDGPRAHAYKREEGYILDDSHCVVSDSEAYHLGQEETDWFDKPRDARSDRFRHHGGHTVSSSQKRGPARHSYHDYDEPPEEGLWPHDEGGPGRHTSAKEHRHHGDHGRHSGRHAGEEPGRRAARPHARDMGRHETRPHPQASPAPAMQKKGQPGYPSSADYSQPSRAPSAYHHASDSKKGSRQAHSGPTVLQPKPEAQAQPQMQGRQAVPGPQQSQPPSSRQTPSGTASRQPQTQQQQQQQQQQQQQQQQQQQQGLGQQAPQQAPSQARLQQQSQPTTRSTAPAASHPAGKPQPGPTTAPGPQPAGLPRAEQAGSSKPAAKAPQQGRAPQAQSAPGPAGAKTGARPGGTPGAPAGQPAAEGESVFSKILPGGAAEQAGKLTEAVSAFGKKFSSFW

Target ORF information:

RefSeq Version	NM_019146.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bassoon (presynaptic cytomatrix protein) (Bsn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019146.2

ORF Insert Sequence:

ATGGGCAACG AGGCCAGCCT GGAGGGCGGC GCGGGCGAAG GGCCGCTGCC GCCGGGAGGC 
TCTGGTCTGG GTCCGGGCCC CGGAGCAGGG AAGCCGCCTT CAGCACTAGC TGGCGGCGGA 
CAGCTCCCCG TGGCTGGTGC TGCGCGAGCG GCGGGACCCC CGACCCCTGG TCTCGGTCTG 
GTCCCCGGCC CTGGTCCCGG CCCCGGCCCG GGCAGTGTTT CCCGGAGACT GGACCCCAAG 
GAGCCCCTGG GCAGCCAGAG AGCAACTTCT CCAACCCCAA AGCAAGCTTC TGCTACTGCT 
CCTGGCCGAG AGAGCCCCCG AGAGACAAGG GCCCAAGGCC TGTCAGGCCA GGAGGCCGAA 
GGTCCCCGCA GGACACTGCA GGTAGACAGC AGGACACAGA GATCAGGACG GTCCCCCTCC 
GTGTCACCAG ACAGAGGCAG CACTCCCACA TCACCCTATT CCGTCCCCCA GATTGCCCCT 
CTTCCCAGCA GCACCCTGTG TCCAATATGC AAGACATCGG ACCTCACATC AACCTCCAGC 
CAGCCAAACT TCAACACCTG CACCCAGTGT CACAACAAGG TCTGCAACCA ATGTGGGTTC 
AACCCCAACC CTCACCTCAC CCAGGTGAAG GAGTGGCTCT GTTTGAACTG TCAGATGCAG 
AGGGCCCTGG GAATGGACAT GACCACTGCA CCTCGGTCCA AGAGCCAGCA GCAGCTACAC 
TCCCCGGCCC TGTCTCCTGC CCACTCCCCA GCCAAACAGC CTCTGGGGAA GCCAGAGCAA 
GAGAGATCTC GGAGCCCAGG GGCCACACAG TCTGGTCCCC GCCAGGCTGA AGCAGCCAGG 
GCCACCTCAG TGCCTGGGCC TACCCAGGCA ACTGCCCCTC CAGAGGTGGG GAGGGTGTCT 
CCTCAGCCCC CTCTCTCTAC CAAGCCTTCC ACAGCTGAGC CCAGGCCACC TGCAGGAGAG 
GCCCAGGGCA AAAGTGCCAC CACAGTGCCC TCCGGGCTTG GTGCTGCTGA ACAGACCCAG 
GAGGGGCTCA CCGGGAAGCT CTTTGGCCTT GGAGCATCAC TGCTGACCCA GGCAAGCACC 
CTTATGTCTG TGCAGCCAGA GGCTGACACC CAGGGCCAGC CTTCCCCCAG CAAGGGGCCA 
CCCAAGATTG TCTTCAGTGA TGCCAGCAAG GAGGCGGGTC CAAGACCCCC AGGCTCAGGG 
CCCGGGCCTG GGCCAACCCC TGGAGCCAAA ACGGAGCCTG GGCCTAGAAC AGGTCCTGGA 
TCAGGGCCTG GGGCCCTGGC AAAAACTGGA GGAACCCCCA GTCCAAAGCA TGGCAGAGCA 
GACCATCAGG CAGCATCCAA AGCTGCTGCC AAGCCAAAGA CCATGCCGAA GGAAAGGGCT 
GCCTGCCCAC TGTGCCAAGC CGAGCTCAAC GTGGGTAGCA GGGGCCCAGC CAACTACAAT 
ACCTGCACTG CCTGCAAGCT CCGGGTGTGC ACCCTGTGTG GCTTTAACCC GACACCACAC 
CTGGTGGAGA AAACAGAGTG GCTCTGTCTG AACTGCCAAA CCAAGAGGCT GCTGGAGGGC 
AGCTTGGGGG AGCCGGCCCC CCTGCCTCTG CCCACCCCAC AGCAGCCTCC AGCAGGGGTC 
CCTCAGCGAG CAGCTGGAGC AAGTCCTCTG AAGCAGAAAG GGCCACAGGG GCCGGGCCAG 
CCCTCAGGCT CCCTGCCTCC CAAGGCCAGT CCTCAAGCCG CCAAGGCCAG CCCTCAGGCC 
GCCAAGGCCA GCCCTCAGGC CAAGCCTCTC AGGGCTTCTG AACCCAGCAA GACCTCTAGC 
AGCGCCCCGG AAAAGAAGAC AGGGATCCCT GTGAAAGCTG AGCCTGTGCC GAAGCCACCT 
CCAGAGACTG CTGTGCCTCC TGGGACTCCT AAAGCAAAAA GCGGGGTGAA GAGGACCGAC 
CCTGCCACCC CAGTCGTCAA GCCTGTTCCA GAAGCTCCCA AGAGCGGGGA GGCTGAGGAA 
CCTGTCCCCA AGCCTTACTC TCAGGATCTG TCTCGAAGCC CACAAAGCCT CAGTGATACA 
GGCTACTCCT CCGATGGCGT CTCCAGCTCC CAGAGTGAGA TCACAGGTGT TGTACAGCAA 
GAAGTGGAGC AACTTGACAG TGCGGGGGTG ACAGGCCCAC GTCCACCCAG CCCCTCAGAG 
CTCCACAAAG TGGGCAGTAG TATGCGCCCT TCCCTGGAGG CCCAGGCTGT GGCTCCCAGT 
GGTGAGTGGA GCAAGCCACC CAGCGGCAGT GCTGTTGAAG ACCAGAAACG GCGTCCCCAC 
TCCCTGTCCA TCATGCCCGA GGCCTTCGAC TCTGACGAGG AGCTGGGGGA TATTCTAGAA 
GAGGATGACT CCTTGGCATG GGGGCGTCAG AGGGAGCAGC AAGACACAGC TGAGTCTTCA 
GATGACTTTG GCAGCCAGCT GAGGCATGAC TACGTGGAAG ACAGCAGTGA AGGCGGCTTG 
TCTCCCCTTC CACCTCAGCC CCCAGCCAGG GCAGATATGA CTGATGAAGA GTTCATGCGG 
CGGCAGATCC TAGAAATGAG CGCCGAGGAA GACAACTTGG AGGAAGACGA CACCGCTGTC 
TCCGGGCGAG GCCTGGCCAA ACACGGTGCC CAGAAGGCAA GTGCCAGACC CAGGCCCGAA 
TCTAGCCAAG AATCAGTAGC ACTGCCCAAG AGGCGCCTGC CCCATAATGC CACCACAGGT 
TACGAGGAAC TTCTGTCTGA GGAAGGCCCA GCAGAGCCCA CTGACGGAGC CCTTCAGGGA 
GGTCTCCGAC GCTTTAAAAC TATTGAGCTC AACAGCACAG GTAGCTATGG TCACGAGCTA 
GACCTGGGCC AAGGCTCTGA TCCCAACCTA GACCGGGAGC CTGAGCTGGA GATGGAGAGC 
CTTACAGGCT CGCCTGAAGA CCGCTCTCGT GGGGAGCACT CCTCTACACT GCCGGCTTCC 
ACGCCTAGCT ATACATCTGG CACCTCACCC ACCTCCCTGT CTTCCCTGGA GGAGGACAGT 
GACAGCAGCC CCAGCCGCAG GCAGCGGCTA GAAGAAGCGA AGCAGCAGCG CAAGGCCCGG 
CACCGGTCCC ACGGGCCCCT GCTGCCCACC ATTGAGGACT CTTCCGAGGA GGAGGAGCTG 
CGGGAAGAAG AGGAGCTGCT GCGTGAGCAA GAGAAGATGC GGGAGGTGGA ACAGCAGCGA 
ATTCGAAGCA CAGCCCGCAA GACCCGGCGT GACAAGGAAG AACTTCGGGC TCAGCGGCGG 
CGTGAGCGCT CCAAGACACC GCCTAGTAAC CTGTCACCCA TCGAAGATGC GTCCCCTACA 
GAGGAGCTGA GGCAGGCAGC GGAGATGGAG GAGCTACACC GCTCTTCCTG CTCTGAGTAC 
TCCCCCTCCC CCTCCCTGGA CTCAGAGGCT GAGACCCTCG ATGGCGGCCC CACTCGGCTC 
TACAAGTCGG GCAGCGAGTA CAACCTGCCT GCCTTTATGT CCCTCTGCTC ACCAACCGAG 
ACCCCTTCAG GCAGTTCCAC CACGCCCAGT TCTGGACGGC CCCTTAAGAG TGCGGAGGAG 
GCCTACGAGG ACATGATGAG AAAAGCTGAG CTGCTCCAGA GACAGCAGGG CCAGGCGGCA 
GGGGCCCGGG GACCCCATGG CGGCCCCTCT CAGCCCACAG GCCCCCGCAG CCAGGGCTCC 
TTTGAATACC AAGACACCCA AGACCATGAC TATGGCGGCA GGGCTTCTCA GCCTGCGGCA 
GATGGCACGC CCGCAGGCCT CGGAGCAACA GTGTATGAGG AGATCCTTCA GACATCCCAG 
AGCATAGCCC GGATGCGGCA GGCCTCCTCG CGGGATCTGG CCTTCACTGA GGACAAAAAG 
AAGGAGAAGC AGTTTCTGAA TGCGGAGAGT GCATACATGG ACCCAATGAA GCAAAATGGT 
GGCCCACTTA CCCCTGGGAC TAGCCCCACC CAACTTGCTG CTCCTGTGTC ATTTCCCACT 
TCCACCTCCT CAGACAGCAG CGGGGGCCGA GTTATTCCCG ATGTCAGAGT CACTCAGCAT 
TTTGCAAAAG AGCCTCAGGA GCCTCTCAAG CTACATAGCT CTCCTGCCTC TCCCAGTCTG 
GCCTCTAAGG AGGTAGGCAT GACCTTCTCC CAGGGTCCTG GAACTCCAGC CACCACAGCC 
ATGGCGCCTT GTCCAGCCAG CCTTCCACGG GGGTATATGA CTCCAGCCGG CCCTGAGCGC 
AGCCCATCAA CATCTTCCAC AATACACAGC TATGGACAGC CCCCAACCAC TGCTAACTAT 
GGGTCTCAGA CGGAGGAGCT GCCTCACGCT CCCAGTGGCC CTGCTGGAAG TGGGCGGGCC 
TCCAGAGAGA AGCCTCTGAG TGGGGGTGAT GGTGAGGTTG GTCCCCCCCA GCCTTCTCGG 
GGATATTCTT ATTTTACGGG CTCTAGCCCA CCTCTCTCTC CATCCACCCC CTCCGAGAGC 
CCCACCTTCT CTCCAAGCAA GCTGGGTCCA AGGGCCACAG CAGAGTTCTC TACACAGACG 
CCAAGCCTAA CGCCTTCCTC TGACATTCCA CGGAGCCCTG GCACCCCTTC CCCCATGGTA 
GCCCAAGGCA CGCAGACGCC ACACCGACCT AGCACTCCTC GCTTGGTGTG GCAGCAGTCT 
TCCCAGGAGG CTCCTGTTAT GGTCATCACG CTAGCTTCAG ATGCTTCTAG CCAGACCCGA 
ATGGTACACG CCAGTGCCTC TACTTCCCCT CTGTGCTCGC CTACGGACTC TCAGCCTGCC 
TCCCATAGCT ACAGCCAGAC CACACCCCCA AGTGCATCTC AAATGCCCTC AGAACCAGCT 
GGGCCACCTG GTTTCCCACG AGCACCCAGT GCTGGTGTAG ACGGGCCATT GGCGCTGTAT 
GGCTGGGGTG CCCTCCCTGC TGAAAACATC TCCTTATGCC GGATCTCCTC TGTCCCTGGA 
ACATCTAGAG TTGAGCCGGG CCCCAGGCCC CCGGGCACCG CAGTGGTCGA CCTTCGCACG 
GCTGTCAAGC CCACACCCAT TATCCTTACC GACCAGGGCA TGGATCTGAC CTCTCTTGCT 
GTGGAAGCAA GGAAGTATGG CCTTGCCCTG GATCCAGTTC CAGGCCGCCA GTCAACCGCC 
GTGCAGCCTC TAGTTATTAA CCTCAATGCC CAAGAGCAGA CCCATACCTT CCTGGCCACT 
GCCACCACAG TGAGCATCAC CATGGCCTCA TCTGTGCTCA TGGCGCAGCA AAAGCAACCA 
GTGGTATATG GAGATCCTTT CCAGAGCCGC CTCGACTTTG GCCAGGGTTC AGGTAGCCCT 
GTGTGCCTGG CCCAGGTCAA GCAAGTAGAG CAGGCTGTCC AGACAGCCCC ATACCGAGGT 
GGGCCCAGGG GAAGACCCAG GGAGGCTAAG TTCGCCAGGT ATAACCTTCC CAACCAGGTA 
ACACCTCTGG CCAGAAGGGA CATTCTGATC ACTCAGATGG GCACTGCCCA GAGTGTTAGC 
CTGAAGCCAG GACCAGTGCC AGAGCCTGGT GCTGAACCCC ACCGGGCTAC TCCTGCAGAG 
CTCCGGGCAC ATGCTCTACC AGGTACCAGG AAGCCACACA CAGTGGTGGT GCAGATGGGA 
GAGGGCGCAG CCGGCACGGT GACTACCCTG CTCCCAGAAG AGCCAGCAGG AGCCCTGGAC 
CTCACTGGGA TGAGGCCCGA GAGCCGACTG GCATGCTGTG ACATGGCCTA TAAGTTTCCC 
TTTGGCAGTA GCTGCACTGG TACCTTCCAT CCTGCCCCTA GTGCACCTGA CAAGAGTGTG 
ACAGATGCTG CCCTGCCTGG CCAAAGCAGT GGCCCCTTCT ACAGTCCCAG AGACCCTGAG 
CCTCCTGAGC CCCTCACCTT CCGGGCACAA GGGGTCGTAG GACCTGGGCC CCATGAAGAA 
CAGAGGCCCT ACCCACAGGG CTTGCCCGGT AGGCTCTACT CCTCCATGTC TGACACCAAT 
TTGGCTGAAG CTGGTCTCAA CTACCACGCC CAGAGGATTG GGCAGCTCTT CCAGGGCCCT 
GGGAGGGACT CAGCTGTGGA TCTCAGCTCG CTGAAGCATT CTTACAGCTT AGGCTTCGCT 
GATGGACGCT ACTTGGGGCA GGGCTTACAG TATGGCTCGT TCACGGACCT GCGCCACCCC 
ACAGACCTTT TGTCTCACCC ACTGCCCATG AGGCGCTACA GCTCTGTGTC AAACATCTAT 
TCGGATCACC GGTACGGCCC ACGGGGAGAT GCCGTCGGCT TTCAGGAGGC CAGCCTGGCC 
CAGTACAGTG CCACCACAGC CCGCGAGATC AGCCGCATGT GTGCCGCCCT CAACTCCATG 
GACCAGTACG GTGGGCGGCA TGGCGGTGGA AGTGGTGGGC CCGACCTTGT GCCGTATCAG 
CCCCAGCACG GGCCTGGACT CAATGCTCCA CAGGGTCTGG CTTCCCTTAG ATCTGGCCTC 
CTCGGCAACC CCACCTACCC AGAAGGGCAA CCAAGTCCTG GGAACCTTGC TCAGTACGGG 
CCTGCAGCAA GCCAAGGAAC AGCAGTCAGA CAGTTGCTCC CATCCACAGC AACAGTGCGT 
GCAGCCGATG GCATGATCTA CTCGACTATC AATACCCCAA TCGCTGCAAC ACTGCCCATC 
ACCACCCAGC CTGCCTCAGT ACTGCGGCCC ATGGTGCGTG GTGGCATGTA CAGGCCTTAC 
GGATCTGGCG GAGTCACAGC TGTGCCCCTT ACCAGCCTGA CCCGGGTGCC CATGATTGCC 
CCTCGGGTAC CTCTTGGACC AGCAGGGTTA TACCGCTATC CTGCACCAAG TCGATTCCCC 
ATCGCTTCCA CCATTCCACC CGCTGAAGGA CCTGTCTATT TGGGAAAGCC AGCAGCCGCC 
AAGGCCTCGG GGGCAGGAGG CCCGCCAAGG CCTGAGCTAC CAGCAGGGGG TGCTCGAGAA 
GAACCTCTCT CCACAACTGC CCCTCCTGCT GTCATCAAGG AAGCCCCGGT GGCCCAGGCC 
CCGGCCCCAC CACCAGGCCA AAAGCCAGCA GGAGATGCTG CTGCTGGGAG TGGCAGTGGA 
GTCCTCGGCC GGCCTGTGAT GGAGAAGGAG GAGGCATCTC AGGAGGACCG GCAGCGAAAG 
CAACAGGAGC AGCTGCTGCA GCTGGAGCGG GAACGGGTAG AGTTGGAAAA GCTCCGGCAG 
TTGCGGCTAC AGGAGGAGCT GGAGCGAGAG AGGGTGGAGC TGCAGAGGCA TCGTGAGGAG 
GAGCAGCTGC TGGTGCAGAG AGAGTTGCAG GAGCTCCAGA CCATCAAGCA CCATGTTCTG 
CAGCAGCAGC AAGAAGAGCG CCAAGCTCAG TTCGCACTGC AGCGCGAGCA GCTGGCACAG 
CAGCGGCTGC AGCTAGAGCA GATCCAGCAA CTGCAGCAGC AGCTGCAGCA GCAGCTAGAG 
GAGCAGAAAC AGAGGCAAAA GGCCCCCTTT CCTGCAACCT GTGAGGCGCC TAGCCGAGGG 
CCTCCCCCCG CTGCTACCGA GCTGGCCCAG AACGGCCAGT ATTGGCCACC ATTGACCCAC 
ACAGCCTTCA TCGCCGTGGC AGGAACCGAA GGGCCTGGGC AAGCTCGTGA GCCCGTGCTG 
CACAGGGGCC TCCCCAGCTC TGCCTCAGAC ATGTCCCTGC AAACTGAGGA GCAGTGGGAG 
GCAGGCCGTA GTGGCATCAA GAAACGTCAC TCTATGCCAC GCCTGAGAGA CGCCTGTGAA 
CCAGAGTCAG GCCCTGATCC CAGCACGGTC AGGAGGATTG CAGACAGCAG TGTTCAGACA 
GATGATGAGG AGGGCGAGGG CCGCTACCTC CTGACCCGTA GGCGCAGGAC ACGGCGCAGT 
GCCGACTGCA GTGTGCAGAC AGATGACGAG GACAATGCCG AATGGGAGCA GCCTGTCCGC 
CGCCGCAGGT CTCGTCTTTC CCGTCACTCA GACTCAGGCT CTGACAGCAA GCACGAGGCC 
TCGGCCTCAT CGTCCGCTGC TGCCGCTGCG GCGAGGGCCA TGAGCAGTGT GGGTATCCAG 
ACCATCAGTG ACTGCTCTGT ACAGACAGAG CCTGAGCAGC TGCCCAGGGT CTCACCAGCC 
ATCCACATCA CAGCCGCCAC TGACCCAAAG GTGGAGATCG TCAGATACAT ATCAGCACCA 
GAAAAGACTG GGCGAGGGGA GAGCCTGGCC TGCCAGACAG AACCCGATGG GCAGGCTCAG 
GGTGTGGCTG GGCCACAGCT CATAGGACCA ACTGCCATCA GCCCCTACCT ACCTGGCATC 
CAGATAGTCA CCCCAGGAGC CCTAGGCAGA TTTGAAAAGA AGAAGCCAGA TCCTCTGGAG 
ATTGGGTACC AGGCCCACCT GCCCCCGGAG TCCCTGTCGC AGCTTGTGAG CCGCCAGCCT 
CCCAAGTCTC CACAAGTGCT CTACTCACCA GTGTCGCCAC TGTCCCCACA CCGGCTCCTG 
GACACCTCGT TTGCTTCCAG TGAGAGGCTG AACAAGGCTC ATGTGAGTCC CCAGAAGCAG 
TTCATAGCTG ACAGCACTCT TCGCCAGCAG ACTCTGCCGC GCCCCATGAA GACCCTGCAG 
CGGTCCCTGT CCGACCCTAA GCCCCTGAGC CCCACCGCCG AGGAGTCTGC CAAAGAGAGA 
TTCTCCCTCT ACCAGCACCA GGGGGGACTA GGTAGCCAGG TGTCGGCGCT GCCACCCAAC 
GGCCTGGTCC GCAAGGTGAA GCGGACACTG CCCAGCCCCC CTCCAGAGGA AGCTCACCTT 
CCCCTGGCTG GCCAGGTGCC CTCACAGCTG TATGCAGCCA GTCTGCTGCA GCGAGGGTTG 
GCGGGGCCCA CCACCGTCCC TGCTACCAAG GCCAGCCTGC TCCGGGAGCT GGACCGGGAC 
CTGCGGCTGG TGGAGCATGA ATCCACCAAG CTGCGCAAGA AGCAGGCGGA GTTGGACGAG 
GAGGAGAAGG AGATTGATGC CAAGCTCAAG TACCTGGAGC TGGGCATCAC CCAGCGCAAA 
GAGTCTTTGG CCAAAGACCG GGTTGGCCGT GACTACCCAC CTTTACGTGG CCTTGGTGAG 
CATCGTGACT ACCTGTCTGA CAGCGAGCTC AACCAGCTGC GGCTCCAGGG TTGTACCACG 
CCCGCTGGCC AGTATGTGGA CTACCCTGCC TCAGCTGCTG TGCCTGCCAC CCCCTCTGGC 
CCCACTGCCT TCCAACAGCC CCGGTTCCCA CCTGCAGCCA CACAGTACAC TGCAGGCAGT 
AGTGGACCAA CTCAGAATGG ATTTCTAGCC CACCAGGCAC CTACCTACAC TGGCCCTAGC 
ACGTACCCAG CACCTACCTA CCCTCCTGGA ACGAGTTATC CAGCTGAGCC TGGCCTGCCA 
AGCCAACCGG CTTTCCACCC CACAGGCCAT TACGCAGCCC CAACGCCCAT GCCAACCACG 
CAGAGCGCCC CTTTTCCAGT CCAAGCTGAC AGTCACGCTG CCCACCAGAA GCCGCGCCAG 
ACCTCACTGG CTGATTTGGA GCAGAAGGTT CCCACCAATT ATGAAGTGAT CAGCAGCCCT 
GCTGTAACCG TGTCCTCAAC CCCCTCTGAG ACCGGCTACA GTGGTCCAGC TGTGAGCAGC 
AGCTACGAGC ACGGTAAAGC CCCTGAGCAT CCCCGGGGTG GTGATCGAAG CAGTGTGAGC 
CAGAGCCCAG CCCCTACATA TCCCTCTGAC TCCCATTACA CCAGTCTGGA GCAGAACGTT 
CCCCGAAACT ATGTGATGAT CGATGACATC AGTGAGCTAA CCAAGGACAG CACCCCCACT 
GCCTCGGATA GCCAACGGCC AGAGCCCTTG GGCCCAGGTG GAGTCAGTGG GCGTCCCGGG 
AAGGATCCCG GAGAACCAGC TGTCCTAGAG GGACCCACTC TGCCCTGCTG CTATGGCAGA 
GGTGAGGAAG AATCTGAGGA GGACTCATAT GACCCCCGTG GCAAATCTGG TCATCACCGG 
AGCATGGAGA GCAACGGCCG ACCAGCCAGC ACCCACTATT ACAGTGACAG TGACTATAGA 
CATGGGGCTC GAGCAGACAA GTATGGCCCA GGACCCATGG GGCCAAAGCA TCCCTCTAAG 
AACCTGGCCC CGGCTGCCAT CTCCTCCAAG CGCAGCAAGC ACCGGAAGCA AGGCATGGAG 
CAAAAGATCT CCAAGTTCTC ACCTATCGAG GAGGCCAAGG ACGTGGAATC AGACCTGGCC 
TCCTATCCCC CACCCACCGT CAGCAGCAGC CTGACCTCTC GGAGCAGAAA GTTCCAAGAC 
GAAATCACCT ATGGGCTCAA GAAGAATGTG TATGAACAGC AGAGGTACTA CGGGGTGTCC 
AGCCGGGACA CAGCCGAGGA GGATGACCGC ATGTATGGTG GTAGCAGCCG GTCCCGAGTG 
GCATCGGCAT ACAGTGGGGA GAAGCTGTCC AGCCATGATT TCAGCAGCCG AAGCAAAGGG 
TATGAACGGG AACGGGAGAC TGCAGAGCGA CTTCAAAAGG CGGGGCCCAA GCCCTCATCC 
CTGAGCATGG CTCATGGACG GGCACGGCCC CCCATGAGGA GCCAGGCCTC TGAAGAGGAG 
AGCCCTGTCA GCCCCTTAGG GCGGCCCCGT CCCGCAGGGG GCGCTCTTCC TCCTGGGGAT 
ACCTGCCCAC AGTTCTGCTC CAGCCACTCC ATGCCGGACG TCCAGGAGCA CGTCAAGGAC 
GGACCGCGCG CCCACGCATA TAAGCGTGAG GAGGGCTACA TCTTGGATGA CTCCCACTGC 
GTGGTTTCGG ACAGTGAAGC GTATCACCTG GGCCAGGAGG AGACAGACTG GTTTGATAAG 
CCCCGAGATG CCCGCTCCGA CCGGTTCAGG CACCATGGGG GGCATACAGT CTCCTCCTCC 
CAGAAGCGAG GCCCTGCCAG GCACAGCTAC CATGACTACG ATGAGCCCCC TGAGGAGGGC 
CTGTGGCCTC ATGATGAGGG TGGTCCAGGC CGGCATACCT CAGCCAAGGA GCACCGGCAC 
CACGGTGACC ACGGGAGGCA CTCAGGCCGC CATGCTGGTG AGGAGCCAGG GCGCCGTGCT 
GCCAGACCAC ATGCTCGGGA CATGGGTCGC CACGAGACCC GGCCTCACCC TCAGGCCAGC 
CCTGCCCCTG CCATGCAAAA GAAGGGTCAG CCTGGGTACC CCAGCTCTGC TGATTACTCA 
CAGCCATCCC GGGCTCCGTC AGCATACCAC CATGCCTCTG ACAGCAAAAA GGGCTCCCGG 
CAGGCCCACT CTGGGCCTAC TGTACTGCAG CCAAAGCCAG AAGCCCAGGC ACAGCCACAG 
ATGCAAGGTC GGCAGGCGGT TCCGGGACCG CAGCAATCAC AGCCACCATC GTCCAGGCAG 
ACGCCCTCAG GCACAGCATC ACGCCAGCCA CAGACACAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA ACAGCAGCAA CAGCAAGGTC TTGGGCAACA AGCTCCCCAG 
CAGGCTCCAT CACAGGCTCG GCTACAGCAA CAGAGCCAGC CAACCACCCG GAGCACAGCC 
CCTGCTGCCA GCCACCCAGC AGGGAAACCT CAACCAGGCC CCACCACAGC CCCAGGGCCC 
CAGCCAGCAG GACTGCCACG TGCAGAGCAG GCAGGCAGCT CCAAACCGGC AGCCAAAGCG 
CCCCAGCAGG GGCGCGCTCC TCAGGCCCAG TCAGCTCCAG GACCTGCAGG GGCGAAGACT 
GGAGCCAGGC CTGGGGGGAC CCCAGGGGCT CCTGCTGGTC AGCCAGCGGC AGAAGGAGAG 
AGTGTATTTT CTAAGATCCT CCCTGGTGGG GCAGCAGAGC AAGCCGGAAA GCTGACAGAA 
GCTGTCTCTG CTTTTGGCAA AAAATTTTCC TCATTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon.
PloS one11(12)e0167535(2016)
Terry-Lorenzo RT,Torres VI,Wagh D,Galaz J,Swanson SK,Florens L,Washburn MP,Waites CL,Gundelfinger ED,Reimer RJ,Garner CC

Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover.
Neuron87(3)521-33(2015 Aug)
K?rber C,Horstmann H,Venkataramani V,Herrmannsd?rfer F,Kremer T,Kaiser M,Schwenger DB,Ahmed S,Dean C,Dresbach T,Kuner T

Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo.
The EMBO journal34(8)1056-77(2015 Apr)
Ivanova D,Dirks A,Montenegro-Venegas C,Sch?ne C,Altrock WD,Marini C,Frischknecht R,Schanze D,Zenker M,Gundelfinger ED,Fejtova A

Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone.
Frontiers in synaptic neuroscience719(2015)
Gundelfinger ED,Reissner C,Garner CC

Localization of the presynaptic cytomatrix protein Piccolo at ribbon and conventional synapses in the rat retina: comparison with Bassoon.
The Journal of comparative neurology439(2)224-34(2001 Oct)
Dick O,Hack I,Altrock WD,Garner CC,Gundelfinger ED,Brandst?tter JH