G0s2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	G0s2
Entrez Gene ID	289388
Full Name	G0/G1switch 2
General protein information	Preferred Names G0/G1switch 2 Names G0/G1 switch protein 2 G0/G1 switch gene 2 G0S2-like protein putative lymphocyte G0/G1 switch
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q27

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009632.1	NP_001009632.1	G0/G1 switch protein 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	G0S2	NP_056529.1
Rattus norvegicus (Norway rat)	G0s2	NP_001009632.1
Mus musculus (house mouse)	G0s2	NP_032085.1
Bos taurus (cattle)	G0S2	NP_001179076.1
Canis lupus familiaris (dog)	G0S2	XP_005622384.1
Pan troglodytes (chimpanzee)	G0S2	XP_514164.1

Gene Ontology
Bibliography

Related articles in PubMed

Liver-specific G
Sugaya Y, Satoh H
Journal of diabetes9(8)754-763(2017 Aug)

Upregulation of Pnpla2 and Abhd5 and downregulation of G0s2 gene expression in mesenteric white adipose tissue as a potential reason for elevated concentration of circulating NEFA after removal of retroperitoneal, epididymal, and inguinal adipose tissue.
Dettlaff-Pokora A, Sledzinski T, Swierczynski J
Molecular and cellular biochemistry422(1-2)21-29(2016 Nov)

Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles.
Turnbull PC, Longo AB, Ramos SV, Roy BD, Ward WE, Peters SJ
American journal of physiology. Regulatory, integrative and comparative physiology310(2)R125-33(2016 Jan)

Lipolytic inhibitor G0/G1 switch gene 2 inhibits reactive oxygen species production and apoptosis in endothelial cells.
Wang Y, Zhang Y, Zhu Y, Zhang P
American journal of physiology. Cell physiology308(6)C496-504(2015 Mar)

Characterization of lipolytic inhibitor G(0)/G(1) switch gene-2 protein (G0S2) expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase (ATGL) and comparitive gene identification-58 (CGI-58).
Turnbull PC, Ramos SV, MacPherson RE, Roy BD, Peters SJ
PloS one10(3)e0120136(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

upregulation of Pnpla2 expression and activation of ATGL (due to an increase in ABHD5 and decrease in G0S2 levels), as well as a coordinated interplay of these genes with Lipe in mesenteric WAT, contribute, at least in part, to an increase in the concentration of circulating NEFA in rats with reduced fat mass.
Title: Upregulation of Pnpla2 and Abhd5 and downregulation of G0s2 gene expression in mesenteric white adipose tissue as a potential reason for elevated concentration of circulating NEFA after removal of retroperitoneal, epididymal, and inguinal adipose tissue.

Report increased levels of ATGL and its inhibitor G0S2 following endurance training in metabolically different rat skeletal muscles.
Title: Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles.

Results indicate that G0S2 acts as a prosurvival molecule in endothelial cells.
Title: Lipolytic inhibitor G0/G1 switch gene 2 inhibits reactive oxygen species production and apoptosis in endothelial cells.

Administration of progesterone resulted in an increase in the expression of Abhd5 gene (whose product increases ATGL activity) and G0s2 gene (whose product decreases ATGL activity) in the inguinal white adipose tissue of female rats.
Title: Progesterone-induced down-regulation of hormone sensitive lipase (Lipe) and up-regulation of G0/G1 switch 2 (G0s2) genes expression in inguinal adipose tissue of female rats is reflected by diminished rate of lipolysis.

These data suggest that the content of G0S2 relative to the lipase in skeletal muscle would not predict lipolytic potential.
Title: Characterization of lipolytic inhibitor G(0)/G(1) switch gene-2 protein (G0S2) expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase (ATGL) and comparitive gene identification-58 (CGI-58).

The following G0s2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the G0s2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12840	NM_001009632.1 Latest version!	Rattus norvegicus G0/G1switch 2 (G0s2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

G0s2 ( NM_001009632.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009632.1 Rattus norvegicus G0/G1switch 2 (G0s2), mRNA.

Price & Availability↑

CloneID	ORa12840
Clone ID Related Accession (Same CDS sequence)	NM_001009632.1
Accession Version	NM_001009632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-06
Organism	Rattus norvegicus(Norway rat)
Product	G0/G1 switch protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088248.1. On Jan 13, 2005 this sequence version replaced XM_213979.2. ##Evidence-Data-START## Transcript exon combination :: BC088248.1, CO560140.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAGTG TGCAGGAGCT GATCCCTCTG GCTAAGGAGA TGATGACGCA GAAGTCCCGA 
GGGAAGCTAG TGAAGCTGTA CGTGCTGGGC AGTGTGCTAG CGTTCTTCGG TGTGGTGCTC 
GGTCTAGTGG AGACTGTGTG CAGCCCTTTC ACAGCTGCCA GCCGTCTGCG CGACCAGGAG 
GCGGCAGTGG CAGAGCTGCG GGATGCTTGT GAGCAGCAGT CCCTCCACAA GCAGGCCCTG 
ATAGCAGAAG GCAAGACACA GGAGGCGACT TTGTGCAGCC GGGCCCTGTC CCTCCGGCAG 
CATGCCTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009632.1
CDS	97..408
Translation	MESVQELIPLAKEMMTQKSRGKLVKLYVLGSVLAFFGVVLGLVETVCSPFTAASRLRDQEAAVAELRDACEQQSLHKQALIAEGKTQEATLCSRALSLRQHAS

Target ORF information:

RefSeq Version	NM_001009632.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G0/G1switch 2 (G0s2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009632.1

ORF Insert Sequence:

ATGGAAAGTG TGCAGGAGCT GATCCCTCTG GCTAAGGAGA TGATGACGCA GAAGTCCCGA 
GGGAAGCTAG TGAAGCTGTA CGTGCTGGGC AGTGTGCTAG CGTTCTTCGG TGTGGTGCTC 
GGTCTAGTGG AGACTGTGTG CAGCCCTTTC ACAGCTGCCA GCCGTCTGCG CGACCAGGAG 
GCGGCAGTGG CAGAGCTGCG GGATGCTTGT GAGCAGCAGT CCCTCCACAA GCAGGCCCTG 
ATAGCAGAAG GCAAGACACA GGAGGCGACT TTGTGCAGCC GGGCCCTGTC CCTCCGGCAG 
CATGCCTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Liver-specific G
Journal of diabetes9(8)754-763(2017 Aug)
Sugaya Y,Satoh H

Upregulation of Pnpla2 and Abhd5 and downregulation of G0s2 gene expression in mesenteric white adipose tissue as a potential reason for elevated concentration of circulating NEFA after removal of retroperitoneal, epididymal, and inguinal adipose tissue.
Molecular and cellular biochemistry422(1-2)21-29(2016 Nov)
Dettlaff-Pokora A,Sledzinski T,Swierczynski J

Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles.
American journal of physiology. Regulatory, integrative and comparative physiology310(2)R125-33(2016 Jan)
Turnbull PC,Longo AB,Ramos SV,Roy BD,Ward WE,Peters SJ

Lipolytic inhibitor G0/G1 switch gene 2 inhibits reactive oxygen species production and apoptosis in endothelial cells.
American journal of physiology. Cell physiology308(6)C496-504(2015 Mar)
Wang Y,Zhang Y,Zhu Y,Zhang P

Characterization of lipolytic inhibitor G(0)/G(1) switch gene-2 protein (G0S2) expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase (ATGL) and comparitive gene identification-58 (CGI-58).
PloS one10(3)e0120136(2015)
Turnbull PC,Ramos SV,MacPherson RE,Roy BD,Peters SJ