Mapkapk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mapkapk2
Entrez Gene ID	289014
Full Name	mitogen-activated protein kinase-activated protein kinase 2
General protein information	Preferred Names mitogen-activated protein kinase-activated protein kinase 2 Names MAP kinase-activated protein kinase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q13
Summary	mediates formation of actin stress fibers in septal neuronal cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178102.2	NP_835203.1	MAP kinase-activated protein kinase 2
XM_017598692.1	XP_017454181.1	MAP kinase-activated protein kinase 2 isoform X1

Pathways from BioSystems

Homologies

	MAPKAPK2	XP_001085549.1
Canis lupus familiaris (dog)	MAPKAPK2	XP_005622356.1
Pan troglodytes (chimpanzee)	MAPKAPK2	XP_001164988.1
Rattus norvegicus (Norway rat)	Mapkapk2	NP_835203.1
Xenopus tropicalis (tropical clawed frog)	mapkapk2	NP_001017347.1
Mus musculus (house mouse)	Mapkapk2	NP_032577.1
Homo sapiens (human)	MAPKAPK2	NP_116584.2
Bos taurus (cattle)	MAPKAPK2	XP_002693936.3
Gallus gallus (chicken)	MAPKAPK2	XP_417976.4
Danio rerio (zebrafish)	mapkapk2a	NP_958460.1

Gene Ontology
Bibliography

Related articles in PubMed

Roles of MAPKAPK-2 and HSP27 in the reduction of renal ischemia-reperfusion injury by ischemic postconditioning in rats.
Xia A, Li Y, Li N, Xue Z, Xia J, Wei T, Cao J
International urology and nephrology46(7)1455-64(2014 Jul)

Endoplasmic reticulum-associated ubiquitin-conjugating enzyme Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in MK2-mediated TNF? production.
Menon MB, Tiedje C, Lafera J, Ronkina N, Konen T, Kotlyarov A, Gaestel M
The Biochemical journal456(2)163-72(2013 Dec)

Developmental profiling of spiral ganglion neurons reveals insights into auditory circuit assembly.
Lu CC, Appler JM, Houseman EA, Goodrich LV
The Journal of neuroscience : the official journal of the Society for Neuroscience31(30)10903-18(2011 Jul)

Silencing mitogen-activated protein kinase-activated protein kinase-2 arrests inflammatory bone loss.
Li Q, Yu H, Zinna R, Martin K, Herbert B, Liu A, Rossa C Jr, Kirkwood KL
The Journal of pharmacology and experimental therapeutics336(3)633-42(2011 Mar)

DNA damage activates a spatially distinct late cytoplasmic cell-cycle checkpoint network controlled by MK2-mediated RNA stabilization.
Reinhardt HC, Hasskamp P, Schmedding I, Morandell S, van Vugt MA, Wang X, Linding R, Ong SE, Weaver D, Carr SA, Yaffe MB
Molecular cell40(1)34-49(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

is suggested that renal protective effect may be related to MAPKAPK-2 activation in the reduction of renal ischemia-reperfusion injury by ischemic postconditioning
Title: Roles of MAPKAPK-2 and HSP27 in the reduction of renal ischemia-reperfusion injury by ischemic postconditioning in rats.

MK2 small interfering RNA delivery arrests lipopolysaccharide-induced inflammatory bone loss, decreases inflammatory infiltrate, and decreases osteoclastogenesis.
Title: Silencing mitogen-activated protein kinase-activated protein kinase-2 arrests inflammatory bone loss.

MAPKAPK-2, one of the alpha-BC upstream kinases, and its phosphorylated form were upregulated gradually and peaked 4 days after ischemia/reperfusion injury.
Title: Co-induction of alphaB-crystallin and MAPKAPK-2 in astrocytes in the penumbra after transient focal cerebral ischemia.

p38 MAPK-MAPKAPK-2 pathway mediates Akt activation by angiotensin II in these cells by recruiting active MAPKAPK-2 to a signaling complex that includes both Akt and p38 MAPK.
Title: Role of p38 MAPK and MAPKAPK-2 in angiotensin II-induced Akt activation in vascular smooth muscle cells.

The following Mapkapk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mapkapk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa58009	XM_017598692.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase-activated protein kinase 2 (Mapkapk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa04131	NM_178102.2 Latest version!	Rattus norvegicus MAPK activated protein kinase 2 (Mapkapk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mapkapk2 ( XM_017598692.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454181.1 Rattus norvegicus mitogen-activated protein kinase-activated protein kinase 2 (Mapkapk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58009
Clone ID Related Accession (Same CDS sequence)	XM_017598692.1
Accession Version	XM_017598692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	MAP kinase-activated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 16% of CDS bases ##RefSeq-Attributes-END##

ATGGCCTCAT CTTCAGTCCT GTCGCGTCAG CAGCAATACT TGGCCACCGT TCGAGATAGC 
CACGTTGACT TCATTCTAGT CAGCGCCCAG TCTTCACCTT ATCGAAGGCC TGCGTGCCTT 
CCTGCCTCGC GCCAGTCCGT CTGCTCCCTG GCCTGTTTCC CTGCCAGCTC AGACTGCAGA 
CCGCTCAGGG CTGTTGGAGA GTGTGATGTC AGGTCACCAC CCGCCCCGGC CCAGCCGCCG 
CCGCCGTTCC CTCAGTTCCA CGTCAAGTCG GGCCTGCAGA TCCGAAAGAA CGCCATCACC 
GACGACTACA AGGTCACCAG CCAAGTGCTT GGCCTGGGCA TCAACGGGAA GGTGCTGCGG 
ATCTTCGACA AGAGAACCCA GCAAAAATTC GCCCTAAAGA TGCTGCAGGA CTGTCCCAAG 
GCACGCAGAG AGGTGGAGCT GCACTGGAGG GCCTCCCAGT GCCCACACAT CGTGCACATC 
GTGGACGTCT ATGAGAACCT GTATGCCGGG AGGAAGTGCT TGCTGATTGT CATGGAGTGT 
CTCGATGGTG GAGAGCTCTT TAGTCGGATC CAGGACCGAG GAGACCAGGC ATTCACAGAA 
AGAGAGGCAT CAGAGATCAT GAAGAGCATC GGGGAGGCCA TCCAGTACCT GCACTCTATC 
AACATCGCTC ACCGGGACGT CAAGCCCGAG AACCTCTTAT ACACTTCCAA AAGACCCAAT 
GCCATCCTGA AACTCACTGA TTTTGGCTTT GCCAAGGAAA CCACCAGTCA CAACTCTTTG 
ACCACTCCGT GTTATACACC GTACTATGTG GCTCCGGAAG TACTGGGCCC AGAGAAGTAT 
GACAAGTCCT GTGACATGTG GTCCTTGGGT GTCATCATGT ATATTTTGCT GTGTGGGTAT 
CCCCCCTTCT ATTCCAACCA CGGCCTTGCC ATCTCTCCGG GCATGAAGAC TCGCATTCGA 
ATGGGCCAGT ATGAATTTCC TAACCCAGAA TGGTCAGAAG TATCAGAAGA AGTGAAGATG 
CTTATTCGGA ATCTGCTGAA AACAGAGCCC ACCCAGAGAA TGACCATCAC AGAATTCATG 
AACCACCCGT GGATCATGCA ATCTACGAAG GTCCCTCAGA CTCCACTGCA CACCAGCCGC 
GTCCTGAAGG AGGACAAGGA ACGATGGGAG GATGTCAAGG AGGAGATGAC CAGTGCCTTG 
GCCACGATGC GTGTCGACTA TGAGCAGGTC AAGATAAAGA AGATAGAGGA CGCATCCAAC 
CCTCTGCTTC TCAAGAGGAG GAAGAAAGCT CGCGCCGTGG AAGACGCGGC CCTTGCCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454181.1
CDS	1..1323
Translation	MASSSVLSRQQQYLATVRDSHVDFILVSAQSSPYRRPACLPASRQSVCSLACFPASSDCRPLRAVGECDVRSPPAPAQPPPPFPQFHVKSGLQIRKNAITDDYKVTSQVLGLGINGKVLRIFDKRTQQKFALKMLQDCPKARREVELHWRASQCPHIVHIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQVKIKKIEDASNPLLLKRRKKARAVEDAALAH

Target ORF information:

RefSeq Version	XM_017598692.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase-activated protein kinase 2 (Mapkapk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598692.1

ORF Insert Sequence:

ATGGCCTCAT CTTCAGTCCT GTCGCGTCAG CAGCAATACT TGGCCACCGT TCGAGATAGC 
CACGTTGACT TCATTCTAGT CAGCGCCCAG TCTTCACCTT ATCGAAGGCC TGCGTGCCTT 
CCTGCCTCGC GCCAGTCCGT CTGCTCCCTG GCCTGTTTCC CTGCCAGCTC AGACTGCAGA 
CCGCTCAGGG CTGTTGGAGA GTGTGATGTC AGGTCACCAC CCGCCCCGGC CCAGCCGCCG 
CCGCCGTTCC CTCAGTTCCA CGTCAAGTCG GGCCTGCAGA TCCGAAAGAA CGCCATCACC 
GACGACTACA AGGTCACCAG CCAAGTGCTT GGCCTGGGCA TCAACGGGAA GGTGCTGCGG 
ATCTTCGACA AGAGAACCCA GCAAAAATTC GCCCTAAAGA TGCTGCAGGA CTGTCCCAAG 
GCACGCAGAG AGGTGGAGCT GCACTGGAGG GCCTCCCAGT GCCCACACAT CGTGCACATC 
GTGGACGTCT ATGAGAACCT GTATGCCGGG AGGAAGTGCT TGCTGATTGT CATGGAGTGT 
CTCGATGGTG GAGAGCTCTT TAGTCGGATC CAGGACCGAG GAGACCAGGC ATTCACAGAA 
AGAGAGGCAT CAGAGATCAT GAAGAGCATC GGGGAGGCCA TCCAGTACCT GCACTCTATC 
AACATCGCTC ACCGGGACGT CAAGCCCGAG AACCTCTTAT ACACTTCCAA AAGACCCAAT 
GCCATCCTGA AACTCACTGA TTTTGGCTTT GCCAAGGAAA CCACCAGTCA CAACTCTTTG 
ACCACTCCGT GTTATACACC GTACTATGTG GCTCCGGAAG TACTGGGCCC AGAGAAGTAT 
GACAAGTCCT GTGACATGTG GTCCTTGGGT GTCATCATGT ATATTTTGCT GTGTGGGTAT 
CCCCCCTTCT ATTCCAACCA CGGCCTTGCC ATCTCTCCGG GCATGAAGAC TCGCATTCGA 
ATGGGCCAGT ATGAATTTCC TAACCCAGAA TGGTCAGAAG TATCAGAAGA AGTGAAGATG 
CTTATTCGGA ATCTGCTGAA AACAGAGCCC ACCCAGAGAA TGACCATCAC AGAATTCATG 
AACCACCCGT GGATCATGCA ATCTACGAAG GTCCCTCAGA CTCCACTGCA CACCAGCCGC 
GTCCTGAAGG AGGACAAGGA ACGATGGGAG GATGTCAAGG AGGAGATGAC CAGTGCCTTG 
GCCACGATGC GTGTCGACTA TGAGCAGGTC AAGATAAAGA AGATAGAGGA CGCATCCAAC 
CCTCTGCTTC TCAAGAGGAG GAAGAAAGCT CGCGCCGTGG AAGACGCGGC CCTTGCCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mapkapk2 ( NM_178102.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_835203.1 Rattus norvegicus MAPK activated protein kinase 2 (Mapkapk2), mRNA.

Price & Availability↑

CloneID	ORa04131
Clone ID Related Accession (Same CDS sequence)	NM_178102.2
Accession Version	NM_178102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Rattus norvegicus(Norway rat)
Product	MAP kinase-activated protein kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY197741.1. On or before Dec 17, 2003 this sequence version replaced XM_213873.2, NM_178102.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY197741.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCTGTCGG GCTCTCCGGG CCAGACTCCG CCGGCGCCGT TCCCCAGCCC GCCACCACCC 
GCCCCGGCCC AGCCGCCGCC GCCGTTCCCT CAGTTCCACG TCAAGTCGGG CCTGCAGATC 
CGAAAGAACG CCATCACCGA CGACTACAAG GTCACCAGCC AAGTGCTTGG CCTGGGCATC 
AACGGGAAGG TGCTGCGGAT CTTCGACAAG AGAACCCAGC AAAAATTCGC CCTAAAGATG 
CTGCAGGACT GTCCCAAGGC ACGCAGAGAG GTGGAGCTGC ACTGGAGGGC CTCCCAGTGC 
CCACACATCG TGCACATCGT GGACGTCTAT GAGAACCTGT ATGCCGGGAG GAAGTGCTTG 
CTGATTGTCA TGGAGTGTCT CGATGGTGGA GAGCTCTTTA GTCGGATCCA GGACCGAGGA 
GACCAGGCAT TCACAGAAAG AGAGGCATCA GAGATCATGA AGAGCATCGG GGAGGCCATC 
CAGTACCTGC ACTCTATCAA CATCGCTCAC CGGGACGTCA AGCCCGAGAA CCTCTTATAC 
ACTTCCAAAA GACCCAATGC CATCCTGAAA CTCACTGATT TTGGCTTTGC CAAGGAAACC 
ACCAGTCACA ACTCTTTGAC CACTCCGTGT TATACACCGT ACTATGTGGC TCCGGAAGTA 
CTGGGCCCAG AGAAGTATGA CAAGTCCTGT GACATGTGGT CCTTGGGTGT CATCATGTAT 
ATTTTGCTGT GTGGGTATCC CCCCTTCTAT TCCAACCACG GCCTTGCCAT CTCTCCGGGC 
ATGAAGACTC GCATTCGAAT GGGCCAGTAT GAATTTCCCA ACCCAGAATG GTCAGAAGTA 
TCAGAAGAAG TGAAGATGCT TATTCGGAAT CTGCTGAAAA CAGAGCCCAC CCAGAGAATG 
ACCATCACAG AATTCATGAA CCACCCGTGG ATCATGCAAT CTACGAAGGT CCCTCAGACT 
CCACTGCACA CCAGCCGCGT CCTGAAGGAG GACAAGGAAC GATGGGAGGA TGTCAAGGAG 
GAGATGACCA GTGCCTTGGC CACGATGCGT GTCGACTATG AGCAGGTCAA GATAAAGAAG 
ATAGAGGACG CATCCAACCC TCTGCTTCTC AAGAGGAGGA AGAAAGCTCG CGCCGTGGAA 
GACGCGGCCC TTGCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835203.1
CDS	302..1462
Translation	MLSGSPGQTPPAPFPSPPPPAPAQPPPPFPQFHVKSGLQIRKNAITDDYKVTSQVLGLGINGKVLRIFDKRTQQKFALKMLQDCPKARREVELHWRASQCPHIVHIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQVKIKKIEDASNPLLLKRRKKARAVEDAALAH

Target ORF information:

RefSeq Version	NM_178102.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MAPK activated protein kinase 2 (Mapkapk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178102.2

ORF Insert Sequence:

ATGCTGTCGG GCTCTCCGGG CCAGACTCCG CCGGCGCCGT TCCCCAGCCC GCCACCACCC 
GCCCCGGCCC AGCCGCCGCC GCCGTTCCCT CAGTTCCACG TCAAGTCGGG CCTGCAGATC 
CGAAAGAACG CCATCACCGA CGACTACAAG GTCACCAGCC AAGTGCTTGG CCTGGGCATC 
AACGGGAAGG TGCTGCGGAT CTTCGACAAG AGAACCCAGC AAAAATTCGC CCTAAAGATG 
CTGCAGGACT GTCCCAAGGC ACGCAGAGAG GTGGAGCTGC ACTGGAGGGC CTCCCAGTGC 
CCACACATCG TGCACATCGT GGACGTCTAT GAGAACCTGT ATGCCGGGAG GAAGTGCTTG 
CTGATTGTCA TGGAGTGTCT CGATGGTGGA GAGCTCTTTA GTCGGATCCA GGACCGAGGA 
GACCAGGCAT TCACAGAAAG AGAGGCATCA GAGATCATGA AGAGCATCGG GGAGGCCATC 
CAGTACCTGC ACTCTATCAA CATCGCTCAC CGGGACGTCA AGCCCGAGAA CCTCTTATAC 
ACTTCCAAAA GACCCAATGC CATCCTGAAA CTCACTGATT TTGGCTTTGC CAAGGAAACC 
ACCAGTCACA ACTCTTTGAC CACTCCGTGT TATACACCGT ACTATGTGGC TCCGGAAGTA 
CTGGGCCCAG AGAAGTATGA CAAGTCCTGT GACATGTGGT CCTTGGGTGT CATCATGTAT 
ATTTTGCTGT GTGGGTATCC CCCCTTCTAT TCCAACCACG GCCTTGCCAT CTCTCCGGGC 
ATGAAGACTC GCATTCGAAT GGGCCAGTAT GAATTTCCCA ACCCAGAATG GTCAGAAGTA 
TCAGAAGAAG TGAAGATGCT TATTCGGAAT CTGCTGAAAA CAGAGCCCAC CCAGAGAATG 
ACCATCACAG AATTCATGAA CCACCCGTGG ATCATGCAAT CTACGAAGGT CCCTCAGACT 
CCACTGCACA CCAGCCGCGT CCTGAAGGAG GACAAGGAAC GATGGGAGGA TGTCAAGGAG 
GAGATGACCA GTGCCTTGGC CACGATGCGT GTCGACTATG AGCAGGTCAA GATAAAGAAG 
ATAGAGGACG CATCCAACCC TCTGCTTCTC AAGAGGAGGA AGAAAGCTCG CGCCGTGGAA 
GACGCGGCCC TTGCCCACTG A