Supt4h1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Supt4h1
Entrez Gene ID	287608
Full Name	SPT4 homolog, DSIF elongation factor subunit
Synonyms	Supt4h2
General protein information	Preferred Names SPT4 homolog, DSIF elongation factor subunit Names transcription elongation factor SPT4 suppressor of Ty 4 homolog 1 suppressor of Ty 4 homolog 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105828.2	NP_001099298.2	transcription elongation factor SPT4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SUPT4H1	XP_523811.2
	SUPT4H1	XP_001106749.1
Drosophila melanogaster (fruit fly)	spt4	NP_610802.1
Caenorhabditis elegans (roundworm)	spt-4	NP_497135.1
Mus musculus (house mouse)	Supt4a	NP_033322.1
Danio rerio (zebrafish)	supt4h1	NP_001002509.1
Bos taurus (cattle)	SUPT4H1	NP_001029964.1
Rattus norvegicus (Norway rat)	Supt4h1	NP_001099298.2
Neurospora crassa	NCU03838	XP_960597.1
Xenopus tropicalis (tropical clawed frog)	supt4h1	NP_001016679.1
Magnaporthe oryzae (rice blast fungus)	MGG_06943	XP_003709683.1
Homo sapiens (human)	SUPT4H1	NP_003159.1
Canis lupus familiaris (dog)	SUPT4H1	XP_853156.2
Gallus gallus (chicken)	SUPT4H1	NP_001244215.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005428	XP_315434.1

Gene Ontology
Bibliography

Related articles in PubMed

Control of transcriptional elongation.
Kwak H, Lis JT
Annual review of genetics47483-508(2013)

RNA polymerase I activity is regulated at multiple steps in the transcription cycle: recent insights into factors that influence transcription elongation.
Schneider DA
Gene493(2)176-84(2012 Feb)

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Flynn RA, Almada AE, Zamudio JR, Sharp PA
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)

Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro.
Wada T, Takagi T, Yamaguchi Y, Watanabe D, Handa H
The EMBO journal17(24)7395-403(1998 Dec)

DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs.
Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H
Genes & development12(3)343-56(1998 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Supt4h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Supt4h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11488	NM_001105828.2 Latest version!	Rattus norvegicus SPT4 homolog, DSIF elongation factor subunit (Supt4h1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Supt4h1 ( NM_001105828.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099298.2 Rattus norvegicus SPT4 homolog, DSIF elongation factor subunit (Supt4h1), mRNA.

Price & Availability↑

CloneID	ORa11488
Clone ID Related Accession (Same CDS sequence)	NM_001105828.2
Accession Version	NM_001105828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	transcription elongation factor SPT4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168730.1. On Sep 21, 2008 this sequence version replaced NM_001105828.1. ##Evidence-Data-START## Transcript exon combination :: BC168730.1, FQ213593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCTGG AGACGGTGCC AAAGGACCTG CGGCATCTGC GAGCTTGTTT GCTGTGCTCG 
TTAGTCAAGA CTATAGACCA GTTTGAATAT GATGGGTGTG ACAATTGCGA TGCATACCTA 
CAAATGAAGG GCAACAGAGA GATGGTGTAC GACTGCACCA GCTCTTCATT TGATGGAATC 
ATCGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGCGT CAGTAACTTT 
AAGCCAGGTG TATATGCTGT GTCAGTCACT GGTCGTCTGC CCCAAGGAAT TGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCG AGAGACACAG CAATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099298.2
CDS	21..374
Translation	MALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Target ORF information:

RefSeq Version	NM_001105828.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPT4 homolog, DSIF elongation factor subunit (Supt4h1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105828.2

ORF Insert Sequence:

ATGGCCCTGG AGACGGTGCC AAAGGACCTG CGGCATCTGC GAGCTTGTTT GCTGTGCTCG 
TTAGTCAAGA CTATAGACCA GTTTGAATAT GATGGGTGTG ACAATTGCGA TGCATACCTA 
CAAATGAAGG GCAACAGAGA GATGGTGTAC GACTGCACCA GCTCTTCATT TGATGGAATC 
ATCGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGCGT CAGTAACTTT 
AAGCCAGGTG TATATGCTGT GTCAGTCACT GGTCGTCTGC CCCAAGGAAT TGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCG AGAGACACAG CAATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Control of transcriptional elongation.
Annual review of genetics47483-508(2013)
Kwak H,Lis JT

RNA polymerase I activity is regulated at multiple steps in the transcription cycle: recent insights into factors that influence transcription elongation.
Gene493(2)176-84(2012 Feb)
Schneider DA

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)
Flynn RA,Almada AE,Zamudio JR,Sharp PA

Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro.
The EMBO journal17(24)7395-403(1998 Dec)
Wada T,Takagi T,Yamaguchi Y,Watanabe D,Handa H

DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs.
Genes & development12(3)343-56(1998 Feb)
Wada T,Takagi T,Yamaguchi Y,Ferdous A,Imai T,Hirose S,Sugimoto S,Yano K,Hartzog GA,Winston F,Buratowski S,Handa H