Carhsp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Carhsp1
Entrez Gene ID	260416
Full Name	calcium regulated heat stable protein 1
Synonyms	Crhsp-24,Crhsp24
General protein information	Preferred Names calcium regulated heat stable protein 1 Names calcium-regulated heat stable protein 1 calcium-regulated heat-stable protein (24kD) calcium-regulated heat-stable protein of 24 kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	phosphoprotein and calcineurin substrate; involved in calcium-mediated signal transduction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245753.3	XP_006245815.1	calcium-regulated heat stable protein 1 isoform X2
XM_006245750.3	XP_006245812.1	calcium-regulated heat stable protein 1 isoform X1
NM_152790.2	NP_690003.1	calcium-regulated heat stable protein 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Carhsp1	NP_690003.1
Homo sapiens (human)	CARHSP1	NP_001265194.1
	CARHSP1	XP_001102514.1
Canis lupus familiaris (dog)	CARHSP1	XP_005621618.1
Bos taurus (cattle)	CARHSP1	NP_001039416.1
Mus musculus (house mouse)	Carhsp1	NP_080097.1
Danio rerio (zebrafish)	carhsp1	NP_956856.1
Drosophila melanogaster (fruit fly)	CG9705	NP_001261972.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005641	XP_315660.4
Xenopus tropicalis (tropical clawed frog)	carhsp1	XP_002939490.2
Pan troglodytes (chimpanzee)	CARHSP1	XP_003315010.1
Gallus gallus (chicken)	CARHSP1	XP_414938.2

Gene Ontology
Bibliography

Related articles in PubMed

CARHSP1 is required for effective tumor necrosis factor alpha mRNA stabilization and localizes to processing bodies and exosomes.
Pfeiffer JR, McAvoy BL, Fecteau RE, Deleault KM, Brooks SA
Molecular and cellular biology31(2)277-86(2011 Jan)

Identification of calcineurin regulated phosphorylation sites on CRHSP-24.
Lee S, Wishart MJ, Williams JA
Biochemical and biophysical research communications385(3)413-7(2009 Jul)

Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites.
Hoffert JD, Pisitkun T, Wang G, Shen RF, Knepper MA
Proceedings of the National Academy of Sciences of the United States of America103(18)7159-64(2006 May)

Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.
Moser K, White FM
Journal of proteome research5(1)98-104(2006 Jan)

Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL.
Auld GC, Campbell DG, Morrice N, Cohen P
The Biochemical journal389(Pt 3)775-83(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Coordinate phosphorylation of four serines explains the multiple phosphorylated isoforms of CRHSP-24 present in acinar cells.
Title: Identification of calcineurin regulated phosphorylation sites on CRHSP-24.

The purification and identification of CRHSP24 in rat liver extracts, and its role as a substrate for multiple enzymes are reported.
Title: Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL.

data indicate that calcium-regulated heat-stable protein-24 is regulated by diverse and physiologically relevant signaling pathways in pancreatic acinar cells
Title: CRHSP-24 phosphorylation is regulated by multiple signaling pathways in pancreatic acinar cells.

The following Carhsp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Carhsp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02934	NM_152790.2 Latest version!	Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa32719	XM_006245750.3 Latest version!	Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa32722	XM_006245753.3 Latest version!	Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Carhsp1 ( NM_152790.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_690003.1 Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), mRNA.

Price & Availability↑

CloneID	ORa02934
Clone ID Related Accession (Same CDS sequence)	NM_152790.2
Accession Version	NM_152790.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	calcium-regulated heat stable protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071173.1. On Jul 8, 2004 this sequence version replaced NM_152790.1. ##Evidence-Data-START## Transcript exon combination :: BC071173.1, FQ132975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCATCTG AGCCTCCCCC GCCACCGCAG CCTCCCACGC ATCAGACGTC TATAGGGCTA 
TTGGACACCC CTCGTGCCCG GGATCGCTCT CCATCCCCTC TGCGGGGCAA CGTCGTCCCT 
AGCCCTTTGC CTACCCGCCG GACAAGGACC TTTTCAGCGA CGGTGCGGGC TTCACAGGGC 
CCTGTCTACA AAGGAGTCTG TAAATGCTTC TGCCGGTCCA AGGGTCACGG CTTCATCACC 
CCGGCTGATG GTGGGCCTGA CATCTTCCTG CACATCTCTG ATGTGGAAGG GGAGTATGTT 
CCCGTGGAAG GCGACGAGGT CACCTATAAG ATGTGCTCTA TCCCGCCCAA GAATGAGAAA 
CTGCAAGCTG TAGAGGTGGT CATCACCCAC CTGGCACCAG GGACGAAGCA TGAGACCTGG 
TCCGGGCACG TCATCAGCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690003.1
CDS	46..489
Translation	MSSEPPPPPQPPTHQTSIGLLDTPRARDRSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS

Target ORF information:

RefSeq Version	NM_152790.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152790.2

ORF Insert Sequence:

ATGTCATCTG AGCCTCCCCC GCCACCGCAG CCTCCCACGC ATCAGACGTC TATAGGGCTA 
TTGGACACCC CTCGTGCCCG GGATCGCTCT CCATCCCCTC TGCGGGGCAA CGTCGTCCCT 
AGCCCTTTGC CTACCCGCCG GACAAGGACC TTTTCAGCGA CGGTGCGGGC TTCACAGGGC 
CCTGTCTACA AAGGAGTCTG TAAATGCTTC TGCCGGTCCA AGGGTCACGG CTTCATCACC 
CCGGCTGATG GTGGGCCTGA CATCTTCCTG CACATCTCTG ATGTGGAAGG GGAGTATGTT 
CCCGTGGAAG GCGACGAGGT CACCTATAAG ATGTGCTCTA TCCCGCCCAA GAATGAGAAA 
CTGCAAGCTG TAGAGGTGGT CATCACCCAC CTGGCACCAG GGACGAAGCA TGAGACCTGG 
TCCGGGCACG TCATCAGCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Carhsp1 ( XM_006245750.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245812.1 Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32719
Clone ID Related Accession (Same CDS sequence)	XM_006245750.3
Accession Version	XM_006245750.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calcium-regulated heat stable protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245750.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTCAC CGTGCTGTCA TGGGAGGATG GGTGGGGCCC ACTCTCCCTC TCTGGGCCAA 
CCTTCTTACC AGAACTCCTG GCATTGGAAC ATTCTAGGAC TCTATGACCT GTGCTGGAGA 
CCTGACGGCC AGGCCAGCAG CCAGGACCTC TTCAAGGTGG GGGCTTCTCT GAATTTGTGG 
TCCTGCCTGG CTGGGGCCAC AGCCGAGAAG AGGTCAGCCA TGTCATCTGA GCCTCCCCCG 
CCACCGCAGC CTCCCACGCA TCAGACGTCT ATAGGGCTAT TGGACACCCC TCGTGCCCGG 
GATCGCTCTC CATCCCCTCT GCGGGGCAAC GTCGTCCCTA GCCCTTTGCC TACCCGCCGG 
ACAAGGACCT TTTCAGCGAC GGTGCGGGCT TCACAGGGCC CTGTCTACAA AGGAGTCTGT 
AAATGCTTCT GCCGGTCCAA GGGTCACGGC TTCATCACCC CGGCTGATGG TGGGCCTGAC 
ATCTTCCTGC ACATCTCTGA TGTGGAAGGG GAGTATGTTC CCGTGGAAGG CGACGAGGTC 
ACCTATAAGA TGTGCTCTAT CCCGCCCAAG AATGAGAAAC TGCAAGCTGT AGAGGTGGTC 
ATCACCCACC TGGCACCAGG GACGAAGCAT GAGACCTGGT CCGGGCACGT CATCAGCTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245812.1
CDS	603..1265
Translation	MVSPCCHGRMGGAHSPSLGQPSYQNSWHWNILGLYDLCWRPDGQASSQDLFKVGASLNLWSCLAGATAEKRSAMSSEPPPPPQPPTHQTSIGLLDTPRARDRSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS

Target ORF information:

RefSeq Version	XM_006245750.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245750.3

ORF Insert Sequence:

ATGGTCTCAC CGTGCTGTCA TGGGAGGATG GGTGGGGCCC ACTCTCCCTC TCTGGGCCAA 
CCTTCTTACC AGAACTCCTG GCATTGGAAC ATTCTAGGAC TCTATGACCT GTGCTGGAGA 
CCTGACGGCC AGGCCAGCAG CCAGGACCTC TTCAAGGTGG GGGCTTCTCT GAATTTGTGG 
TCCTGCCTGG CTGGGGCCAC AGCCGAGAAG AGGTCAGCCA TGTCATCTGA GCCTCCCCCG 
CCACCGCAGC CTCCCACGCA TCAGACGTCT ATAGGGCTAT TGGACACCCC TCGTGCCCGG 
GATCGCTCTC CATCCCCTCT GCGGGGCAAC GTCGTCCCTA GCCCTTTGCC TACCCGCCGG 
ACAAGGACCT TTTCAGCGAC GGTGCGGGCT TCACAGGGCC CTGTCTACAA AGGAGTCTGT 
AAATGCTTCT GCCGGTCCAA GGGTCACGGC TTCATCACCC CGGCTGATGG TGGGCCTGAC 
ATCTTCCTGC ACATCTCTGA TGTGGAAGGG GAGTATGTTC CCGTGGAAGG CGACGAGGTC 
ACCTATAAGA TGTGCTCTAT CCCGCCCAAG AATGAGAAAC TGCAAGCTGT AGAGGTGGTC 
ATCACCCACC TGGCACCAGG GACGAAGCAT GAGACCTGGT CCGGGCACGT CATCAGCTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Carhsp1 ( XM_006245753.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245815.1 Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa32722
Clone ID Related Accession (Same CDS sequence)	XM_006245753.3
Accession Version	XM_006245753.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calcium-regulated heat stable protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTTG AAATCTCATG GAGCCCTGGC CCAGTGCAGT TTGTATGTGT TGGGTCAGCC 
ATGTCATCTG AGCCTCCCCC GCCACCGCAG CCTCCCACGC ATCAGACGTC TATAGGGCTA 
TTGGACACCC CTCGTGCCCG GGATCGCTCT CCATCCCCTC TGCGGGGCAA CGTCGTCCCT 
AGCCCTTTGC CTACCCGCCG GACAAGGACC TTTTCAGCGA CGGTGCGGGC TTCACAGGGC 
CCTGTCTACA AAGGAGTCTG TAAATGCTTC TGCCGGTCCA AGGGTCACGG CTTCATCACC 
CCGGCTGATG GTGGGCCTGA CATCTTCCTG CACATCTCTG ATGTGGAAGG GGAGTATGTT 
CCCGTGGAAG GCGACGAGGT CACCTATAAG ATGTGCTCTA TCCCGCCCAA GAATGAGAAA 
CTGCAAGCTG TAGAGGTGGT CATCACCCAC CTGGCACCAG GGACGAAGCA TGAGACCTGG 
TCCGGGCACG TCATCAGCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245815.1
CDS	135..638
Translation	MNLEISWSPGPVQFVCVGSAMSSEPPPPPQPPTHQTSIGLLDTPRARDRSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS

Target ORF information:

RefSeq Version	XM_006245753.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium regulated heat stable protein 1 (Carhsp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245753.3

ORF Insert Sequence:

ATGAACCTTG AAATCTCATG GAGCCCTGGC CCAGTGCAGT TTGTATGTGT TGGGTCAGCC 
ATGTCATCTG AGCCTCCCCC GCCACCGCAG CCTCCCACGC ATCAGACGTC TATAGGGCTA 
TTGGACACCC CTCGTGCCCG GGATCGCTCT CCATCCCCTC TGCGGGGCAA CGTCGTCCCT 
AGCCCTTTGC CTACCCGCCG GACAAGGACC TTTTCAGCGA CGGTGCGGGC TTCACAGGGC 
CCTGTCTACA AAGGAGTCTG TAAATGCTTC TGCCGGTCCA AGGGTCACGG CTTCATCACC 
CCGGCTGATG GTGGGCCTGA CATCTTCCTG CACATCTCTG ATGTGGAAGG GGAGTATGTT 
CCCGTGGAAG GCGACGAGGT CACCTATAAG ATGTGCTCTA TCCCGCCCAA GAATGAGAAA 
CTGCAAGCTG TAGAGGTGGT CATCACCCAC CTGGCACCAG GGACGAAGCA TGAGACCTGG 
TCCGGGCACG TCATCAGCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CARHSP1 is required for effective tumor necrosis factor alpha mRNA stabilization and localizes to processing bodies and exosomes.
Molecular and cellular biology31(2)277-86(2011 Jan)
Pfeiffer JR,McAvoy BL,Fecteau RE,Deleault KM,Brooks SA

Identification of calcineurin regulated phosphorylation sites on CRHSP-24.
Biochemical and biophysical research communications385(3)413-7(2009 Jul)
Lee S,Wishart MJ,Williams JA

Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites.
Proceedings of the National Academy of Sciences of the United States of America103(18)7159-64(2006 May)
Hoffert JD,Pisitkun T,Wang G,Shen RF,Knepper MA

Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.
Journal of proteome research5(1)98-104(2006 Jan)
Moser K,White FM

Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL.
The Biochemical journal389(Pt 3)775-83(2005 Aug)
Auld GC,Campbell DG,Morrice N,Cohen P