Cd81 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd81
Entrez Gene ID	25621
Full Name	Cd81 molecule
Synonyms	Tapa1
General protein information	Preferred Names Cd81 molecule Names CD81 antigen CD 81 antigen CD81 antigen (target of antiproliferative antibody 1) target of the antiproliferative antibody 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41-q42
Summary	may play a role in glial scar formation in response to astrocyte injury; may be involved in regulation of cell interactions and cell growth [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013087.2	NP_037219.2	CD81 antigen

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CD81	NP_001025510.1
Danio rerio (zebrafish)	zgc:92411	NP_001003735.1
Xenopus tropicalis (tropical clawed frog)	cd81	NP_989271.1
Pan troglodytes (chimpanzee)	CD81	NP_001009023.1
	CD81	XP_001093228.1
Bos taurus (cattle)	CD81	NP_001030271.1
Drosophila melanogaster (fruit fly)	Tsp96F	NP_524500.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000823	XP_316787.2
Homo sapiens (human)	CD81	NP_004347.1
Canis lupus familiaris (dog)	LOC611606	XP_005631797.1
Mus musculus (house mouse)	Cd81	NP_598416.1
Danio rerio (zebrafish)	cd81	NP_571593.2
Rattus norvegicus (Norway rat)	Cd81	NP_037219.1

Gene Ontology
Bibliography

Related articles in PubMed

CD81 association with SAMHD1 enhances HIV-1 reverse transcription by increasing dNTP levels.
Rocha-Perugini V, Su?rez H, ?lvarez S, L?pez-Mart?n S, Lenzi GM, Vences-Catal?n F, Levy S, Kim B, Mu?oz-Fern?ndez MA, S?nchez-Madrid F, Y??ez-M? M
Nature microbiology2(11)1513-1522(2017 Nov)

The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin ?v?3.
Yu J, Lee CY, Changou CA, Cedano-Prieto DM, Takada YK, Takada Y
The Biochemical journal474(4)589-596(2017 02)

Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia.
Shen L, Diao Z, Sun HX, Yan GJ, Wang Z, Li RT, Dai Y, Wang J, Li J, Ding H, Zhao G, Zheng M, Xue P, Liu M, Zhou Y, Hu Y
Proceedings of the National Academy of Sciences of the United States of America114(8)1940-1945(2017 Feb)

Crystal Structure of a Full-Length Human Tetraspanin Reveals a Cholesterol-Binding Pocket.
Zimmerman B, Kelly B, McMillan BJ, Seegar TCM, Dror RO, Kruse AC, Blacklow SC
Cell167(4)1041-1051.e11(2016 11)

CD81 promotes both the degradation of transferrin receptor 2 (TfR2) and the Tfr2-mediated maintenance of hepcidin expression.
Chen J, Enns CA
The Journal of biological chemistry290(12)7841-50(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings strongly suggest that CD63 and CD81 play an important role in Schwann cell spreading along axons but seem to be dispensable for Schwann cell myelination
Title: Tetraspanins are involved in Schwann cell-axon interaction.

CD81 exerts significant inhibitory effect, dose-dependently and time-dependently, on the proliferation of astrocytes
Title: CD81 inhibits the proliferation of astrocytes by inducing G(0)/G (1) arrest in vitro.

Data suggest that CD81 does not function as a binding receptor by itself but promotes photoreceptor outer segment particle binding through functional interaction specifically with alpha v beta5 integrin.
Title: Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.

Induction of CD81 expression through HDAC inhibition is a novel strategy to promote growth arrest in glioma cells.
Title: CD81, a cell cycle regulator, is a novel target for histone deacetylase inhibition in glioma cells.

results support the hypothesis that CD81 is associated with final stages of retinal pigment epithelium cell maturation, establishing key molecular interactions linking cell membrane proteins into macromolecular complexes containing PDZ protein scaffolds
Title: The developmental regulation of CD81 in the rat retina.

The following Cd81 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd81 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa43178	NM_013087.2 Latest version!	Rattus norvegicus Cd81 molecule (Cd81), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd81 ( NM_013087.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037219.2 Rattus norvegicus Cd81 molecule (Cd81), mRNA.

Price & Availability↑

CloneID	ORa43178
Clone ID Related Accession (Same CDS sequence)	NM_013087.2
Accession Version	NM_013087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Rattus norvegicus(Norway rat)
Product	CD81 antigen
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM032840.1, FN799324.1, FM090203.1 and CR476007.1. On Apr 28, 2015 this sequence version replaced NM_013087.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ215739.1, FQ214006.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGTGG AAGGCTGCAC CAAATGCATC AAATACCTGC TCTTCGTCTT CAATTTCGTC 
TTCTGGCTGG CTGGAGGTGT GATCCTAGGT GTAGCTCTGT GGTTGCGCCA TGATCCACAA 
ACCACCAGCC TTCTCTACCT GGAACTGGGA GACAAACCAG CACCTAGCAC CTTCTATGTG 
GGCATCTACA TTCTCATTGC TGTGGGAGCT GTGATGATGT TTGTAGGCTT CCTGGGGTGC 
TATGGGGCCA TCCAGGAGTC CCAGTGCCTG CTGGGGACGT TCTTCACTTG CCTTGTGATC 
CTGTTTGCCT GTGAGGTAGC TGCGGGCATC TGGGGCTTTG TAAACAAAGA CCAGATCGCC 
AAGGATGTGA AGCAGTTCTA CGACCAGGCC CTTCAGCAGG CTGTGATGGA TGATGATGCC 
AACAATGCCA AGGCAGTGGT GAAGACCTTC CATGAGACGC TCAACTGTTG TGGCTCCAAT 
ACGCTGACCA CACTGACCAC CACCGTGCTG AGGAACAGCC TGTGTCCCTC AAGCAGCAAC 
TCATTCACTC AGCTTCTGAA GGAAGACTGC CATCAGAAAA TCGATGAGCT CTTTTCTGGG 
AAGCTGTACC TCATTGGAAT TGCAGCCATT GTGGTAGCTG TCATTATGAT CTTCGAGATG 
ATTCTGAGCA TGGTGCTGTG CTGTGGCATC CGGAACAGCT CCGTGTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037219.2
CDS	249..959
Translation	MGVEGCTKCIKYLLFVFNFVFWLAGGVILGVALWLRHDPQTTSLLYLELGDKPAPSTFYVGIYILIAVGAVMMFVGFLGCYGAIQESQCLLGTFFTCLVILFACEVAAGIWGFVNKDQIAKDVKQFYDQALQQAVMDDDANNAKAVVKTFHETLNCCGSNTLTTLTTTVLRNSLCPSSSNSFTQLLKEDCHQKIDELFSGKLYLIGIAAIVVAVIMIFEMILSMVLCCGIRNSSVY

Target ORF information:

RefSeq Version	NM_013087.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cd81 molecule (Cd81), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013087.2

ORF Insert Sequence:

ATGGGGGTGG AAGGCTGCAC CAAATGCATC AAATACCTGC TCTTCGTCTT CAATTTCGTC 
TTCTGGCTGG CTGGAGGTGT GATCCTAGGT GTAGCTCTGT GGTTGCGCCA TGATCCACAA 
ACCACCAGCC TTCTCTACCT GGAACTGGGA GACAAACCAG CACCTAGCAC CTTCTATGTG 
GGCATCTACA TTCTCATTGC TGTGGGAGCT GTGATGATGT TTGTAGGCTT CCTGGGGTGC 
TATGGGGCCA TCCAGGAGTC CCAGTGCCTG CTGGGGACGT TCTTCACTTG CCTTGTGATC 
CTGTTTGCCT GTGAGGTAGC TGCGGGCATC TGGGGCTTTG TAAACAAAGA CCAGATCGCC 
AAGGATGTGA AGCAGTTCTA CGACCAGGCC CTTCAGCAGG CTGTGATGGA TGATGATGCC 
AACAATGCCA AGGCAGTGGT GAAGACCTTC CATGAGACGC TCAACTGTTG TGGCTCCAAT 
ACGCTGACCA CACTGACCAC CACCGTGCTG AGGAACAGCC TGTGTCCCTC AAGCAGCAAC 
TCATTCACTC AGCTTCTGAA GGAAGACTGC CATCAGAAAA TCGATGAGCT CTTTTCTGGG 
AAGCTGTACC TCATTGGAAT TGCAGCCATT GTGGTAGCTG TCATTATGAT CTTCGAGATG 
ATTCTGAGCA TGGTGCTGTG CTGTGGCATC CGGAACAGCT CCGTGTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CD81 association with SAMHD1 enhances HIV-1 reverse transcription by increasing dNTP levels.
Nature microbiology2(11)1513-1522(2017 Nov)
Rocha-Perugini V,Su?rez H,?lvarez S,L?pez-Mart?n S,Lenzi GM,Vences-Catal?n F,Levy S,Kim B,Mu?oz-Fern?ndez MA,S?nchez-Madrid F,Y??ez-M? M

The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin ?v?3.
The Biochemical journal474(4)589-596(2017 02)
Yu J,Lee CY,Changou CA,Cedano-Prieto DM,Takada YK,Takada Y

Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia.
Proceedings of the National Academy of Sciences of the United States of America114(8)1940-1945(2017 Feb)
Shen L,Diao Z,Sun HX,Yan GJ,Wang Z,Li RT,Dai Y,Wang J,Li J,Ding H,Zhao G,Zheng M,Xue P,Liu M,Zhou Y,Hu Y

Crystal Structure of a Full-Length Human Tetraspanin Reveals a Cholesterol-Binding Pocket.
Cell167(4)1041-1051.e11(2016 11)
Zimmerman B,Kelly B,McMillan BJ,Seegar TCM,Dror RO,Kruse AC,Blacklow SC

CD81 promotes both the degradation of transferrin receptor 2 (TfR2) and the Tfr2-mediated maintenance of hepcidin expression.
The Journal of biological chemistry290(12)7841-50(2015 Mar)
Chen J,Enns CA