Tmod1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmod1
Entrez Gene ID	25566
Full Name	tropomodulin 1
Synonyms	E-Tmod,Tmod
General protein information	Preferred Names tropomodulin 1 Names tropomodulin-1 E-Tropomodulin erythrocyte tropomodulin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22
Summary	blocks the elongation and depolymerization of the actin filaments at the pointed end [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017593162.1	XP_017448651.1	tropomodulin-1 isoform X1
NM_013044.2	NP_037176.2	tropomodulin-1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tmod1	NP_068683.1
Drosophila melanogaster (fruit fly)	tmod	NP_001247372.1
Caenorhabditis elegans (roundworm)	unc-94	NP_491734.1
Gallus gallus (chicken)	TMOD1	NP_990358.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000969	XP_003437160.1
Pan troglodytes (chimpanzee)	TMOD1	XP_520136.3
	TMOD1	XP_001114190.1
Canis lupus familiaris (dog)	TMOD1	XP_532002.2
Homo sapiens (human)	TMOD1	NP_001159588.1
Bos taurus (cattle)	TMOD1	NP_001073108.1
Danio rerio (zebrafish)	tmod1	XP_001920602.1
Rattus norvegicus (Norway rat)	Tmod1	NP_037176.2
Xenopus tropicalis (tropical clawed frog)	tmod1	XP_004910958.1

Gene Ontology
Bibliography

Related articles in PubMed

Tropomodulin isoforms utilize specific binding functions to modulate dendrite development.
Gray KT, Suchowerska AK, Bland T, Colpan M, Wayman G, Fath T, Kostyukova AS
Cytoskeleton (Hoboken, N.J.)73(6)316-28(2016 Jun)

How Leiomodin and Tropomodulin use a common fold for different actin assembly functions.
Boczkowska M, Rebowski G, Kremneva E, Lappalainen P, Dominguez R
Nature communications68314(2015 Sep)

Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy.
Yuen M, Sandaradura SA, Dowling JJ, Kostyukova AS, Moroz N, Quinlan KG, Lehtokari VL, Ravenscroft G, Todd EJ, Ceyhan-Birsoy O, Gokhin DS, Maluenda J, Lek M, Nolent F, Pappas CT, Novak SM, D'Amico A, Malfatti E, Thomas BP, Gabriel SB, Gupta N, Daly MJ, Ilkovski B, Houweling PJ, Davidson AE, Swanson LC, Brownstein CA, Gupta VA, Medne L, Shannon P, Martin N, Bick DP, Flisberg A, Holmberg E, Van den Bergh P, Lapunzina P, Waddell LB, Sloboda DD, Bertini E, Chitayat D, Telfer WR, Laquerri?re A, Gregorio CC, Ottenheijm CA, B?nnemann CG, Pelin K, Beggs AH, Hayashi YK, Romero NB, Laing NG, Nishino I, Wallgren-Pettersson C, Melki J, Fowler VM, MacArthur DG, North KN, Clarke NF
The Journal of clinical investigation124(11)4693-708(2014 Nov)

A cardiomyocyte-specific Wdr1 knockout demonstrates essential functional roles for actin disassembly during myocardial growth and maintenance in mice.
Yuan B, Wan P, Chu D, Nie J, Cao Y, Luo W, Lu S, Chen J, Yang Z
The American journal of pathology184(7)1967-80(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tmod1 increases dendritic branching distal from the cell body and the number of filopodia/thin spines. Tmod2 increases dendritic branching proximal to the cell body and the number of mature dendritic spines.
Title: Tropomodulin isoforms utilize specific binding functions to modulate dendrite development.

Identification of residues within tropomodulin-1 responsible for its localization at the pointed ends of the actin filaments in cardiac myocytes.
Title: Identification of residues within tropomodulin-1 responsible for its localization at the pointed ends of the actin filaments in cardiac myocytes.

Lmod contributes to the final organization and maintenance of sarcomere architecture by promoting tropomyosin-dependent actin filament nucleation.
Title: Different localizations and cellular behaviors of leiomodin and tropomodulin in mature cardiomyocyte sarcomeres.

Tmod may be involved in the differentiation process in myogenic cells
Title: Cytoplasmic nuclear transfer of the actin-capping protein tropomodulin.

Introduction of E-Tmod cDNA into a tumor cell line reverses TB10 mediated apoptosis and restored actin architectures.
Title: The interaction between E-tropomodulin and thymosin beta-10 rescues tumor cells from thymosin beta-10 mediated apoptosis by restoring actin architecture.

The following Tmod1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmod1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01758	NM_013044.2 Latest version!	Rattus norvegicus tropomodulin 1 (Tmod1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa01758	XM_017593162.1 Latest version!	Rattus norvegicus tropomodulin 1 (Tmod1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmod1 ( NM_013044.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037176.2 Rattus norvegicus tropomodulin 1 (Tmod1), mRNA.

Price & Availability↑

CloneID	ORa01758
Clone ID Related Accession (Same CDS sequence)	XM_017593162.1 , NM_013044.2
Accession Version	NM_013044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Rattus norvegicus(Norway rat)
Product	tropomodulin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC072521.1. On Jun 13, 2004 this sequence version replaced NM_013044.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCTACA GACGAGAGCT GGAGAAATAT CGAGACCTGG ATGAGGATGA AATCCTGGGG 
GCCCTCACAG AGGAGGAGCT CAGGACACTG GAAAACGAGC TGGATGAGCT AGACCCTGAT 
AATGCACTGC TCCCTGCAGG CCTGAGGCAG AAGGATCAGA CCACCAAGGC ACCCACAGGC 
CCCTTTAAAA GAGAGGAGCT TTTGGACCAC TTGGAAAAGC AAGCGAAGGA ATTTAAGGAC 
CGAGAAGACC TGGTTCCCTA CACAGGGGAG AAACGAGGAA AGATCTGGGT TCCCAAGCAG 
AAGCCAATGG ATCCCGTGCT GGAGAGCGTG ACGCTGGAGC CGGAACTGGA GGAGGCCCTA 
GCAAATGCCT CAGATGCCGA ACTATGTGAC ATTGCAGCTA TTCTGGGCAT GCACACGCTC 
ATGAGCAACC AGCAGTACTA CCAAGCCTTG GGCAGCAGCT CCATCGTGAA CAAGGAGGGG 
CTCAACAGTG TGATCAAACC CACACAGTAC AAGCCTGTGC CTGATGAAGA ACCAAATTCA 
ACAGATGTAG AGGAAACGCT GGAGCGAATA AAGAACAATG ACCCAGAACT TGAAGAGGTT 
AATCTTAACA ACATCCGGAA TATTCCCATA CCCACTCTCA AGGCGTACGC GGAAGCCCTG 
AAAGAGAACT CCTACGTGAA GAAGTTCAGC ATCGTGGGGA CCCGCAGCAA TGACCCTGTG 
GCATTTGCTC TTGCTGAAAT GCTGAAGGTG AACAAGGTGT TGAAGACGCT GAATGTCGAG 
TCCAACTTCA TCTCCGGAGC TGGGATCCTG TGCCTGGTGG AAGCTCTCCC CCACAACACT 
TCTCTGGTCG AACTGAAAAT CGACAACCAG AGCCAGCCTT TGGGCAACAA AGTGGAAATG 
GAGATCGTGA ACATGTTGGA GAAAAACACA ACACTCCTCA AATTTGGCTA CCACTTTACC 
CAGCAGGGGC CCCGGCTGCG GGCATCCAAC GCCATGATGA ACAACAACGA CCTTGTGAGG 
AAGAGGAGGC TCGCAGACCT GACGGGGCCT ATTATTCCCA AGTGCCGCAG CGGTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037176.2
CDS	94..1173
Translation	MSYRRELEKYRDLDEDEILGALTEEELRTLENELDELDPDNALLPAGLRQKDQTTKAPTGPFKREELLDHLEKQAKEFKDREDLVPYTGEKRGKIWVPKQKPMDPVLESVTLEPELEEALANASDAELCDIAAILGMHTLMSNQQYYQALGSSSIVNKEGLNSVIKPTQYKPVPDEEPNSTDVEETLERIKNNDPELEEVNLNNIRNIPIPTLKAYAEALKENSYVKKFSIVGTRSNDPVAFALAEMLKVNKVLKTLNVESNFISGAGILCLVEALPHNTSLVELKIDNQSQPLGNKVEMEIVNMLEKNTTLLKFGYHFTQQGPRLRASNAMMNNNDLVRKRRLADLTGPIIPKCRSGV

Target ORF information:

RefSeq Version	NM_013044.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tropomodulin 1 (Tmod1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013044.2

ORF Insert Sequence:

ATGTCCTACA GACGAGAGCT GGAGAAATAT CGAGACCTGG ATGAGGATGA AATCCTGGGG 
GCCCTCACAG AGGAGGAGCT CAGGACACTG GAAAACGAGC TGGATGAGCT AGACCCTGAT 
AATGCACTGC TCCCTGCAGG CCTGAGGCAG AAGGATCAGA CCACCAAGGC ACCCACAGGC 
CCCTTTAAAA GAGAGGAGCT TTTGGACCAC TTGGAAAAGC AAGCGAAGGA ATTTAAGGAC 
CGAGAAGACC TGGTTCCCTA CACAGGGGAG AAACGAGGAA AGATCTGGGT TCCCAAGCAG 
AAGCCAATGG ATCCCGTGCT GGAGAGCGTG ACGCTGGAGC CGGAACTGGA GGAGGCCCTA 
GCAAATGCCT CAGATGCCGA ACTATGTGAC ATTGCAGCTA TTCTGGGCAT GCACACGCTC 
ATGAGCAACC AGCAGTACTA CCAAGCCTTG GGCAGCAGCT CCATCGTGAA CAAGGAGGGG 
CTCAACAGTG TGATCAAACC CACACAGTAC AAGCCTGTGC CTGATGAAGA ACCAAATTCA 
ACAGATGTAG AGGAAACGCT GGAGCGAATA AAGAACAATG ACCCAGAACT TGAAGAGGTT 
AATCTTAACA ACATCCGGAA TATTCCCATA CCCACTCTCA AGGCGTACGC GGAAGCCCTG 
AAAGAGAACT CCTACGTGAA GAAGTTCAGC ATCGTGGGGA CCCGCAGCAA TGACCCTGTG 
GCATTTGCTC TTGCTGAAAT GCTGAAGGTG AACAAGGTGT TGAAGACGCT GAATGTCGAG 
TCCAACTTCA TCTCCGGAGC TGGGATCCTG TGCCTGGTGG AAGCTCTCCC CCACAACACT 
TCTCTGGTCG AACTGAAAAT CGACAACCAG AGCCAGCCTT TGGGCAACAA AGTGGAAATG 
GAGATCGTGA ACATGTTGGA GAAAAACACA ACACTCCTCA AATTTGGCTA CCACTTTACC 
CAGCAGGGGC CCCGGCTGCG GGCATCCAAC GCCATGATGA ACAACAACGA CCTTGTGAGG 
AAGAGGAGGC TCGCAGACCT GACGGGGCCT ATTATTCCCA AGTGCCGCAG CGGTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmod1 ( XM_017593162.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448651.1 Rattus norvegicus tropomodulin 1 (Tmod1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01758
Clone ID Related Accession (Same CDS sequence)	XM_017593162.1 , NM_013044.2
Accession Version	XM_017593162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tropomodulin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTACA GACGAGAGCT GGAGAAATAT CGAGACCTGG ATGAGGATGA AATCCTGGGG 
GCCCTCACAG AGGAGGAGCT CAGGACACTG GAAAACGAGC TGGATGAGCT AGACCCTGAT 
AATGCACTGC TCCCTGCAGG CCTGAGGCAG AAGGATCAGA CCACCAAGGC ACCCACAGGC 
CCCTTTAAAA GAGAGGAGCT TTTGGACCAC TTGGAAAAGC AAGCGAAGGA ATTTAAGGAC 
CGAGAAGACC TGGTTCCCTA CACAGGGGAG AAACGAGGAA AGATCTGGGT TCCCAAGCAG 
AAGCCAATGG ATCCCGTGCT GGAGAGCGTG ACGCTGGAGC CGGAACTGGA GGAGGCCCTA 
GCAAATGCCT CAGATGCCGA ACTATGTGAC ATTGCAGCTA TTCTGGGCAT GCACACGCTC 
ATGAGCAACC AGCAGTACTA CCAAGCCTTG GGCAGCAGCT CCATCGTGAA CAAGGAGGGG 
CTCAACAGTG TGATCAAACC CACACAGTAC AAGCCTGTGC CTGATGAAGA ACCAAATTCA 
ACAGATGTAG AGGAAACGCT GGAGCGAATA AAGAACAATG ACCCAGAACT TGAAGAGGTT 
AATCTTAACA ACATCCGGAA TATTCCCATA CCCACTCTCA AGGCGTACGC GGAAGCCCTG 
AAAGAGAACT CCTACGTGAA GAAGTTCAGC ATCGTGGGGA CCCGCAGCAA TGACCCTGTG 
GCATTTGCTC TTGCTGAAAT GCTGAAGGTG AACAAGGTGT TGAAGACGCT GAATGTCGAG 
TCCAACTTCA TCTCCGGAGC TGGGATCCTG TGCCTGGTGG AAGCTCTCCC CCACAACACT 
TCTCTGGTCG AACTGAAAAT CGACAACCAG AGCCAGCCTT TGGGCAACAA AGTGGAAATG 
GAGATCGTGA ACATGTTGGA GAAAAACACA ACACTCCTCA AATTTGGCTA CCACTTTACC 
CAGCAGGGGC CCCGGCTGCG GGCATCCAAC GCCATGATGA ACAACAACGA CCTTGTGAGG 
AAGAGGAGGC TCGCAGACCT GACGGGGCCT ATTATTCCCA AGTGCCGCAG CGGTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448651.1
CDS	173..1252
Translation	MSYRRELEKYRDLDEDEILGALTEEELRTLENELDELDPDNALLPAGLRQKDQTTKAPTGPFKREELLDHLEKQAKEFKDREDLVPYTGEKRGKIWVPKQKPMDPVLESVTLEPELEEALANASDAELCDIAAILGMHTLMSNQQYYQALGSSSIVNKEGLNSVIKPTQYKPVPDEEPNSTDVEETLERIKNNDPELEEVNLNNIRNIPIPTLKAYAEALKENSYVKKFSIVGTRSNDPVAFALAEMLKVNKVLKTLNVESNFISGAGILCLVEALPHNTSLVELKIDNQSQPLGNKVEMEIVNMLEKNTTLLKFGYHFTQQGPRLRASNAMMNNNDLVRKRRLADLTGPIIPKCRSGV

Target ORF information:

RefSeq Version	XM_017593162.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tropomodulin 1 (Tmod1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593162.1

ORF Insert Sequence:

ATGTCCTACA GACGAGAGCT GGAGAAATAT CGAGACCTGG ATGAGGATGA AATCCTGGGG 
GCCCTCACAG AGGAGGAGCT CAGGACACTG GAAAACGAGC TGGATGAGCT AGACCCTGAT 
AATGCACTGC TCCCTGCAGG CCTGAGGCAG AAGGATCAGA CCACCAAGGC ACCCACAGGC 
CCCTTTAAAA GAGAGGAGCT TTTGGACCAC TTGGAAAAGC AAGCGAAGGA ATTTAAGGAC 
CGAGAAGACC TGGTTCCCTA CACAGGGGAG AAACGAGGAA AGATCTGGGT TCCCAAGCAG 
AAGCCAATGG ATCCCGTGCT GGAGAGCGTG ACGCTGGAGC CGGAACTGGA GGAGGCCCTA 
GCAAATGCCT CAGATGCCGA ACTATGTGAC ATTGCAGCTA TTCTGGGCAT GCACACGCTC 
ATGAGCAACC AGCAGTACTA CCAAGCCTTG GGCAGCAGCT CCATCGTGAA CAAGGAGGGG 
CTCAACAGTG TGATCAAACC CACACAGTAC AAGCCTGTGC CTGATGAAGA ACCAAATTCA 
ACAGATGTAG AGGAAACGCT GGAGCGAATA AAGAACAATG ACCCAGAACT TGAAGAGGTT 
AATCTTAACA ACATCCGGAA TATTCCCATA CCCACTCTCA AGGCGTACGC GGAAGCCCTG 
AAAGAGAACT CCTACGTGAA GAAGTTCAGC ATCGTGGGGA CCCGCAGCAA TGACCCTGTG 
GCATTTGCTC TTGCTGAAAT GCTGAAGGTG AACAAGGTGT TGAAGACGCT GAATGTCGAG 
TCCAACTTCA TCTCCGGAGC TGGGATCCTG TGCCTGGTGG AAGCTCTCCC CCACAACACT 
TCTCTGGTCG AACTGAAAAT CGACAACCAG AGCCAGCCTT TGGGCAACAA AGTGGAAATG 
GAGATCGTGA ACATGTTGGA GAAAAACACA ACACTCCTCA AATTTGGCTA CCACTTTACC 
CAGCAGGGGC CCCGGCTGCG GGCATCCAAC GCCATGATGA ACAACAACGA CCTTGTGAGG 
AAGAGGAGGC TCGCAGACCT GACGGGGCCT ATTATTCCCA AGTGCCGCAG CGGTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tropomodulin isoforms utilize specific binding functions to modulate dendrite development.
Cytoskeleton (Hoboken, N.J.)73(6)316-28(2016 Jun)
Gray KT,Suchowerska AK,Bland T,Colpan M,Wayman G,Fath T,Kostyukova AS

How Leiomodin and Tropomodulin use a common fold for different actin assembly functions.
Nature communications68314(2015 Sep)
Boczkowska M,Rebowski G,Kremneva E,Lappalainen P,Dominguez R

Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy.
The Journal of clinical investigation124(11)4693-708(2014 Nov)
Yuen M,Sandaradura SA,Dowling JJ,Kostyukova AS,Moroz N,Quinlan KG,Lehtokari VL,Ravenscroft G,Todd EJ,Ceyhan-Birsoy O,Gokhin DS,Maluenda J,Lek M,Nolent F,Pappas CT,Novak SM,D'Amico A,Malfatti E,Thomas BP,Gabriel SB,Gupta N,Daly MJ,Ilkovski B,Houweling PJ,Davidson AE,Swanson LC,Brownstein CA,Gupta VA,Medne L,Shannon P,Martin N,Bick DP,Flisberg A,Holmberg E,Van den Bergh P,Lapunzina P,Waddell LB,Sloboda DD,Bertini E,Chitayat D,Telfer WR,Laquerri?re A,Gregorio CC,Ottenheijm CA,B?nnemann CG,Pelin K,Beggs AH,Hayashi YK,Romero NB,Laing NG,Nishino I,Wallgren-Pettersson C,Melki J,Fowler VM,MacArthur DG,North KN,Clarke NF

A cardiomyocyte-specific Wdr1 knockout demonstrates essential functional roles for actin disassembly during myocardial growth and maintenance in mice.
The American journal of pathology184(7)1967-80(2014 Jul)
Yuan B,Wan P,Chu D,Nie J,Cao Y,Luo W,Lu S,Chen J,Yang Z